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3 results on '"Maternal epigenome"'

1. Maternal blood metal concentrations and whole blood DNA methylation during pregnancy in the Early Autism Risk Longitudinal Investigation (EARLI)

Author

Aung, Max T, Bakulski, Kelly M, Feinberg, Jason I, Dou, John F, Meeker, John D, Mukherjee, Bhramar, Loch-Caruso, Rita, Ladd-Acosta, Christine, Volk, Heather E, Croen, Lisa A, Hertz-Picciotto, Irva, Newschaffer, Craig J, and Fallin, M Daniele

Subjects
Biological Sciences, Genetics, Prevention, Aetiology, 2.2 Factors relating to the physical environment, Reproductive health and childbirth, Autistic Disorder, Basic Helix-Loop-Helix Transcription Factors, DNA Methylation, Epigenome, Female, Genes, Homeobox, Homeodomain Proteins, Humans, Metals, Pregnancy, Maternal epigenome, DNA methylation, trace metals, Biochemistry and Cell Biology, Medical Biochemistry and Metabolomics, Developmental Biology, Biochemistry and cell biology
Abstract

The maternal epigenome may be responsive to prenatal metals exposures. We tested whether metals are associated with concurrent differential maternal whole blood DNA methylation. In the Early Autism Risk Longitudinal Investigation cohort, we measured first or second trimester maternal blood metals concentrations (cadmium, lead, mercury, manganese, and selenium) using inductively coupled plasma mass spectrometry. DNA methylation in maternal whole blood was measured on the Illumina 450 K array. A subset sample of 97 women had both measures available for analysis, all of whom did not report smoking during pregnancy. Linear regression was used to test for site-specific associations between individual metals and DNA methylation, adjusting for cell type composition and confounding variables. Discovery gene ontology analysis was conducted on the top 1,000 sites associated with each metal. We observed hypermethylation at 11 DNA methylation sites associated with lead (FDR False Discovery Rate q-value  0.86). DNA methylation sites associated with lead and manganese may be potential biomarkers of exposure or implicate downstream gene pathways.

Published
2022

2. Maternal blood metal concentrations and whole blood DNA methylation during pregnancy in the Early Autism Risk Longitudinal Investigation (EARLI)

Author

Max T. Aung, Kelly M. Bakulski, Jason I. Feinberg, John F. Dou, John D. Meeker, Bhramar Mukherjee, Rita Loch-Caruso, Christine Ladd-Acosta, Heather E. Volk, Lisa A. Croen, Irva Hertz-Picciotto, Craig J. Newschaffer, and M. Daniele Fallin

Subjects
maternal epigenome, dna methylation, trace metals, Genetics, QH426-470
Abstract

The maternal epigenome may be responsive to prenatal metals exposures. We tested whether metals are associated with concurrent differential maternal whole blood DNA methylation. In the Early Autism Risk Longitudinal Investigation cohort, we measured first or second trimester maternal blood metals concentrations (cadmium, lead, mercury, manganese, and selenium) using inductively coupled plasma mass spectrometry. DNA methylation in maternal whole blood was measured on the Illumina 450 K array. A subset sample of 97 women had both measures available for analysis, all of whom did not report smoking during pregnancy. Linear regression was used to test for site-specific associations between individual metals and DNA methylation, adjusting for cell type composition and confounding variables. Discovery gene ontology analysis was conducted on the top 1,000 sites associated with each metal. We observed hypermethylation at 11 DNA methylation sites associated with lead (FDR False Discovery Rate q-value 0.86). DNA methylation sites associated with lead and manganese may be potential biomarkers of exposure or implicate downstream gene pathways.

Published
2022
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3. Maternal blood metal concentrations and whole blood DNA methylation during pregnancy in the Early Autism Risk Longitudinal Investigation (EARLI)

Author

Jason I. Feinberg, Kelly M. Bakulski, Lisa A. Croen, Rita Loch-Caruso, John F. Dou, Bhramar Mukherjee, Max T. Aung, Heather E. Volk, Christine Ladd-Acosta, Irva Hertz-Picciotto, Craig J. Newschaffer, John D. Meeker, and Daniele Daniele Fallin

Subjects
0301 basic medicine, Cancer Research, trace metals, Physiology, Reproductive health and childbirth, Maternal blood, Biology, Medical Biochemistry and Metabolomics, 03 medical and health sciences, Epigenome, 0302 clinical medicine, Pregnancy, medicine, Basic Helix-Loop-Helix Transcription Factors, Genetics, Humans, Homeobox, 2.2 Factors relating to the physical environment, Autistic Disorder, Aetiology, Molecular Biology, Gene, Whole blood, Homeodomain Proteins, DNA methylation, Prevention, Confounding, Genes, Homeobox, DNA Methylation, medicine.disease, Maternal epigenome, 030104 developmental biology, Genes, Metals, 030220 oncology & carcinogenesis, Cohort, Autism, Female, Biochemistry and Cell Biology, Research Paper, Developmental Biology
Abstract

BackgroundMetals exposures have important health effects in pregnancy. The maternal epigenome may be responsive to these exposures. We tested whether metals are associated with concurrent differential maternal whole blood DNA methylation.MethodsIn the Early Autism Risk Longitudinal Investigation (EARLI) cohort, we measured first or second trimester maternal blood metals concentrations (cadmium, lead, mercury, manganese, and selenium) in 215 participants using inductively coupled plasma mass spectrometry. DNA methylation in maternal whole blood was measured in the same specimens on the Illumina 450K array (201 participants). A subset sample of 97 women had both measures available for analysis, all of whom did not report smoking during pregnancy. Linear regression was used to test for site-specific associations between individual metals and DNA methylation, adjusting for cell type composition and confounding variables. Discovery gene ontology analysis was conducted on the top 1,000 sites associated with each metal to elucidate downstream pathways.ResultsIn multiple linear regression, we observed hypermethylation at 11 DNA methylation sites associated with lead (FDR q-value CYP24A1, ASCL2, FAT1, SNX31, NKX6-2, LRC4C, BMP7, HOXC11, PCDH7, ZSCAN18, and VIPR2. Lead associated sites were enriched (FDR q-value ARID2. Manganese associated sites were enriched for cellular metabolism pathways (FDR q-value0.86).DiscussionSingle DNA methylation sites associated with lead and manganese may be potential biomarkers of exposure or implicate downstream gene pathways. Future studies should replicate our findings to characterize potential toxicological mechanisms of trace metals through the maternal epigenome.

Published
2021
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