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Objective: To characterize the immune cells present in different forms of feline anterior uveitis. Samples: Eyes were obtained from 49 cats diagnosed with chronic idiopathic lymphoplasmacytic anterior uveitis, 7 cats with feline infectious peritonitis (FIP), and 9 cats euthanized for nonocular disease. Methods: H&E sections were scored on the level of infiltrate in the anterior uvea. Immunohistochemistry was performed for FoxP3, CD3, and IL‐17A, and positive cells were quantified in multiple images of each sample. A generalized estimating equation tested for an association between the level of inflammation and the prevalence of these cell types. Results: Cells stained positive for IL‐17A in idiopathic uveitis but not in FIP samples. We found significantly fewer FoxP3+ and CD3+ cells in low‐grade compared with high‐grade inflammation in idiopathic uveitis or FIP samples (P values all <.005), but no difference between FIP and high‐grade samples. Conclusions: Idiopathic, but not FIP‐associated, uveitis appears to have Th17 cell involvement. The numbers of FoxP3+ and CD3+ T‐cells present appear directly correlated; thus, the severity of disease does not appear directly determined by the numbers of regulatory cells. [ABSTRACT FROM AUTHOR]

Purpose To evaluate immediate effects of diamond burr debridement (DBD) on the cornea of canine patients diagnosed with spontaneous chronic corneal epithelial defects (SCCEDs). Animals studied Eight client owned dogs with SCCEDs. Methods Nine eyes from eight dogs with SCCEDs underwent superficial keratectomy (SK). The ulcerated area was divided into quadrants with a 300-micron restricted depth knife. Two of four quadrants underwent DBD for 40-60 s. A SK followed immediately. One burred section and one nonburred section were fixed with formaldehyde 10% and underwent light microscopy (LM). The remaining quadrants from five eyes were fixed with glutaraldehyde 2.5% and underwent transmission electron microscopy (TEM). Masked pathologists evaluated the samples. A student's paired t-test was used to analyze the data. Results With LM all nonburred samples had a superficial stromal hyaline acellular zone (HAZ), seven of the burred samples had an intermittent HAZ and in two burred samples this zone was absent. The HAZ thickness of burred samples (1.062 ± 0.664 μm) was significantly thinner than that of the nonburred samples (4.309 ± 1.348 μm) ( P < 0.0001). Transmission electron microscopy showed an absence of basement membrane and the presence of an amorphous, fine fibrillar material in the superficial stroma in nonburred samples. This material was intermittent or absent in burred samples. Conclusion DBD significantly reduces the superficial stromal HAZ in SCCEDs. A reduction of its thickness may be responsible for the healing rates reported with DBD. [ABSTRACT FROM AUTHOR]

Objectives To review clinical data on dogs that suffered a corneal and anterior segment foreign body ( CASFB) trauma and to determine the risk factors for foreign body ( FB) trauma and subsequent enucleation. Animals studied Dogs with CASFB presented to the Animal Health Trust ( AHT) from January 2000 to December 2012. Procedures Clinical data for CASFB cases were compared to those available for the remaining AHT ophthalmic population over the same period. The depth of the FB trauma was divided into five categories. The type of FB and method of removal were described for each category. The degree of secondary uveitis and lens involvement was graded and correlated with subsequent enucleation. Results The mean age (standard deviation) of 218 identified CASFB cases was 3.96 (2.95) years. Risk factors for CASFB trauma were dogs younger than 5 years, English Springer Spaniels, Labrador Retrievers, and working dogs. Most dogs required general anesthesia for FB removal, and hypodermic needles were the most commonly used instrument. The lens was involved in some cases with a full-thickness CASFB trauma ( n = 49, 45%), but most suffered a minor lens trauma ( n = 37, 76%). The lens trauma and phacoclastic uveitis were managed medically in most dogs ( n = 37, 76%), and phacoemulsification was only elected as initial treatment in some dogs ( n = 10, 20%). Enucleation was required overall in 6% of dogs. Statistically significant associations were found between enucleation and depth of FB trauma, degree of uveitis, and severity of lens trauma ( P < 0.001). Conclusions Young dogs, English Springer Spaniels, Labrador Retrievers, and working dogs had an increased risk of CASFB trauma. Risk factors for enucleation were full-thickness FB penetration, severe lens trauma, and severe uveitis. [ABSTRACT FROM AUTHOR]