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165 results on '"Masuda, Yoshimitsu"'

12. Genetic Characterization, Antibiotic Resistance, and Virulence Genes Profiling of Bacillus cereus Strains from Various Foods in Japan.

Author

Abdelaziz, Marwa Nabil Sayed, Zayda, Mahmoud Gamaleldin, Maung, Aye Thida, El-Telbany, Mohamed, Mohammadi, Tahir Noor, Lwin, Su Zar Chi, Linn, Khin Zar, Wang, Chen, Yuan, Lu, Masuda, Yoshimitsu, Honjoh, Ken-ichi, and Miyamoto, Takahisa

Subjects
TIME-of-flight mass spectrometry, CHICKEN as food, FOOD poisoning, BACILLUS cereus, DRUG resistance in bacteria
Abstract

Bacillus cereus sensu stricto is a foodborne pathogen that causes food poisoning. Their spore and biofilm-forming abilities persist in various environments and foods. This study investigated the prevalence, virulence, antibiotic resistance, and genetic diversity of B. cereus s. s. strains isolated from various food samples. Of 179 samples, 22.34% were positive for B. cereus s. s., with significantly high detection rates in milk products and raw chicken meat. Forty strains were isolated from positive samples. Matrix-assisted laser desorption ionization/time of flight mass spectrometry analysis revealed nine distinct clusters and multi-locus sequence typing revealed 34 sequence types including 23 novel sequences, demonstrating high genetic diversity among the isolates. PCR analysis revealed that all the strains contained at least one toxin gene, but none contained the cytK gene. Antibiotic resistance tests revealed that all isolates were classified as multidrug-resistant, with high resistance levels, particularly to β-lactam antibiotics and vancomycin, but were susceptible to gentamicin. All isolates showed variations in biofilm formation. This study highlights the significant public health risk due to B. cereus s. s. and underscores the need for stringent monitoring and control measures in food production to manage antimicrobial resistance and ensure food safety. [ABSTRACT FROM AUTHOR]

Published
2024
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19. Characterization and antibacterial activity of highly thermo- and pH-stable endolysin LysCPQ7 and its application as a biocontrol agent against Clostridium perfringens in milk and cheese

Author

Noor Mohammadi, Tahir, primary, Lin, Yunzhi, additional, Maung, Aye Thida, additional, Shen, Cunkuan, additional, Zhao, Junxin, additional, El-Telbany, Mohamed, additional, Zayda, Mahmoud, additional, Masuda, Yoshimitsu, additional, Honjoh, Ken-ichi, additional, and Miyamoto, Takahisa, additional

Published
2023
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23. Screening of genes involved in phage-resistance of Escherichia coli and effects of substances interacting with primosomal protein A on the resistant bacteria.

Author

Lin, Chen-Yu, Murayama, Tomoka, Futada, Koshiro, Tanaka, Shota, Masuda, Yoshimitsu, Honjoh, Ken-ichi, and Miyamoto, Takahisa

Abstract

Aims The study was to identify the genes involved in phage resistance and to develop an effective biocontrol method to improve the lytic activity of phages against foodborne pathogens. Methods and results A total of 3,909 single gene-deletion mutants of Escherichia coli BW25113 from the Keio collection were individually screened for genes involved in phage resistance. Phage S127BCL3 isolated from chicken liver, infecting both E. coli BW25113 and O157: H7, was characterized and used for screening. The 10 gene-deletion mutants showed increased susceptibility to phage S127BCL3. Among them, priA gene-deletion mutant strain showed significant susceptibility to the phages S127BCL3 and T7. Furthermore, we investigated the substances that have been reported to inhibit the function of primosomal protein A (PriA) and were used to confirm increased phage susceptibility in E. coli BW25113 (Parent strain) and O157: H7. Conclusion PriA inhibitors at a low concentration showed combined effects with phage against E. coli O157: H7 and delayed the regrowth rate of phage-resistant cells. [ABSTRACT FROM AUTHOR]

Published
2024
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27. Isolation, characterization of Enterococcus phages and their application in control of E. faecalis in milk.

Author

Chen Wang, Junxin Zhao, Yunzhi Lin, Lu Yuan, Mohamed El-Telbany, Aye Thida Maung, Sayed Abdelaziz, Marwa Nabil, Masuda, Yoshimitsu, Ken-ichi Honjoh, and Takahisa Miyamoto

Abstract

Aims: Isolation and characterization of Enterococcus phages and application of phage cocktail to control E. faecalis in milk. Methods and Results: For phage isolations, double layer agar method was used. Host range of the phages were determined by the spot test. Twelve phages with varying host ranges were isolated. Phages PEF1, PEF7b, and PEF9 with different host ranges and lytic activities were selected for phage cocktails. Compared to two-phages cocktails tested, the cocktail containing all the three phages displayed stronger antibacterial and biofilm removal activities. The cocktail treatment reduced viable E. faecalis in biofilm by 6 log within 6 h at both 30◦C and 4◦C. In milk, the cocktail gradually reduced the viable count of E. faecalis and the count reached below the lower limit of detection at 48 h at 4◦C. Conclusion: The strong bactericidal and biofilm removal activities of the phage cocktail suggest the potential of this cocktail as a natural biocontrol agent for combating E. faecalis in milk. [ABSTRACT FROM AUTHOR]

Published
2023
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