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24 results on '"Mascuch, Samantha J"'

2. Parallel deployment of passive and composite samplers for surveillance and variant profiling of SARS-CoV-2 in sewage

Author

Cha, Gyuhyon, Graham, Katherine E., Zhu, Kevin J., Rao, Gouthami, Lindner, Blake G., Kocaman, Kumru, Woo, Seongwook, D'amico, Isabelle, Bingham, Lilia R., Fischer, Jamie M., Flores, Camryn I., Spencer, John W., Yathiraj, Pranav, Chung, Hayong, Biliya, Shweta, Djeddar, Naima, Burton, Liza J., Mascuch, Samantha J., Brown, Joe, Bryksin, Anton, Pinto, Ameet, Hatt, Janet K., and Konstantinidis, Konstantinos T.

Published
2023
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3. Marine Natural Product Honaucin A Attenuates Inflammation by Activating the Nrf2-ARE Pathway

Author

Mascuch, Samantha J, Boudreau, Paul D, Carland, Tristan M, Pierce, N Tessa, Olson, Joshua, Hensler, Mary E, Choi, Hyukjae, Campanale, Joseph, Hamdoun, Amro, Nizet, Victor, Gerwick, William H, Gaasterland, Teresa, and Gerwick, Lena

Subjects
Health Sciences, Traditional, Complementary and Integrative Medicine, Dietary Supplements, Complementary and Integrative Health, Genetics, Nutrition, 1.1 Normal biological development and functioning, 5.1 Pharmaceuticals, 2.1 Biological and endogenous factors, Alkylation, Animals, Anti-Inflammatory Agents, Antioxidants, Aquatic Organisms, Biological Products, Cell Line, Cell Line, Tumor, Cytoprotection, Female, Humans, Inflammation, Kelch-Like ECH-Associated Protein 1, MCF-7 Cells, Mice, NF-E2-Related Factor 2, RAW 264.7 Cells, Signal Transduction, Chemical Sciences, Biological Sciences, Medical and Health Sciences, Medicinal & Biomolecular Chemistry, Traditional, complementary and integrative medicine
Abstract

The cyanobacterial marine natural product honaucin A inhibits mammalian innate inflammation in vitro and in vivo. To decipher its mechanism of action, RNA sequencing was used to evaluate differences in gene expression of cultured macrophages following honaucin A treatment. This analysis led to the hypothesis that honaucin A exerts its anti-inflammatory activity through activation of the cytoprotective nuclear erythroid 2-related factor 2 (Nrf2)-antioxidant response element/electrophile response element (ARE/EpRE) signaling pathway. Activation of this pathway by honaucin A in cultured human MCF7 cells was confirmed using an Nrf2 luciferase reporter assay. In vitro alkylation experiments with the natural product and N-acetyl-l-cysteine suggest that honaucin A activates this pathway through covalent interaction with the sulfhydryl residues of the cytosolic repressor protein Keap1. Honaucin A presents a potential therapeutic lead for diseases with an inflammatory component modulated by Nrf2-ARE.

Published
2018

4. A blueprint for academic laboratories to produce SARS-CoV-2 quantitative RT-PCR test kits

Author

Mascuch, Samantha J., Fakhretaha-Aval, Sara, Bowman, Jessica C., Ma, Minh Thu H., Thomas, Gwendell, Bommarius, Bettina, Ito, Chieri, Zhao, Liangjun, Newnam, Gary P., Matange, Kavita R., Thapa, Hem R., Barlow, Brett, Donegan, Rebecca K., Nguyen, Nguyet A., Saccuzzo, Emily G., Obianyor, Chiamaka T., Karunakaran, Suneesh C., Pollet, Pamela, Rothschild-Mancinelli, Brooke, Mestre-Fos, Santi, Guth-Metzler, Rebecca, Bryksin, Anton V., Petrov, Anton S., Hazell, Mallory, Ibberson, Carolyn B., Penev, Petar I., Mannino, Robert G., Lam, Wilbur A., Garcia, Andrés J., Kubanek, Julia, Agarwal, Vinayak, Hud, Nicholas V., Glass, Jennifer B., Williams, Loren Dean, and Lieberman, Raquel L.

Published
2020
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5. Sharing and community curation of mass spectrometry data with Global Natural Products Social Molecular Networking

Author

Wang, Mingxun, Carver, Jeremy J, Phelan, Vanessa V, Sanchez, Laura M, Garg, Neha, Peng, Yao, Nguyen, Don Duy, Watrous, Jeramie, Kapono, Clifford A, Luzzatto-Knaan, Tal, Porto, Carla, Bouslimani, Amina, Melnik, Alexey V, Meehan, Michael J, Liu, Wei-Ting, Crüsemann, Max, Boudreau, Paul D, Esquenazi, Eduardo, Sandoval-Calderón, Mario, Kersten, Roland D, Pace, Laura A, Quinn, Robert A, Duncan, Katherine R, Hsu, Cheng-Chih, Floros, Dimitrios J, Gavilan, Ronnie G, Kleigrewe, Karin, Northen, Trent, Dutton, Rachel J, Parrot, Delphine, Carlson, Erin E, Aigle, Bertrand, Michelsen, Charlotte F, Jelsbak, Lars, Sohlenkamp, Christian, Pevzner, Pavel, Edlund, Anna, McLean, Jeffrey, Piel, Jörn, Murphy, Brian T, Gerwick, Lena, Liaw, Chih-Chuang, Yang, Yu-Liang, Humpf, Hans-Ulrich, Maansson, Maria, Keyzers, Robert A, Sims, Amy C, Johnson, Andrew R, Sidebottom, Ashley M, Sedio, Brian E, Klitgaard, Andreas, Larson, Charles B, Boya P, Cristopher A, Torres-Mendoza, Daniel, Gonzalez, David J, Silva, Denise B, Marques, Lucas M, Demarque, Daniel P, Pociute, Egle, O'Neill, Ellis C, Briand, Enora, Helfrich, Eric JN, Granatosky, Eve A, Glukhov, Evgenia, Ryffel, Florian, Houson, Hailey, Mohimani, Hosein, Kharbush, Jenan J, Zeng, Yi, Vorholt, Julia A, Kurita, Kenji L, Charusanti, Pep, McPhail, Kerry L, Nielsen, Kristian Fog, Vuong, Lisa, Elfeki, Maryam, Traxler, Matthew F, Engene, Niclas, Koyama, Nobuhiro, Vining, Oliver B, Baric, Ralph, Silva, Ricardo R, Mascuch, Samantha J, Tomasi, Sophie, Jenkins, Stefan, Macherla, Venkat, Hoffman, Thomas, Agarwal, Vinayak, Williams, Philip G, Dai, Jingqui, Neupane, Ram, Gurr, Joshua, Rodríguez, Andrés MC, Lamsa, Anne, Zhang, Chen, Dorrestein, Kathleen, Duggan, Brendan M, Almaliti, Jehad, Allard, Pierre-Marie, and Phapale, Prasad

Subjects
Analytical Chemistry, Chemical Sciences, Generic health relevance, Biological Products, Data Curation, Database Management Systems, Databases, Chemical, Information Dissemination, Information Storage and Retrieval, Internationality, Mass Spectrometry
Abstract

The potential of the diverse chemistries present in natural products (NP) for biotechnology and medicine remains untapped because NP databases are not searchable with raw data and the NP community has no way to share data other than in published papers. Although mass spectrometry (MS) techniques are well-suited to high-throughput characterization of NP, there is a pressing need for an infrastructure to enable sharing and curation of data. We present Global Natural Products Social Molecular Networking (GNPS; http://gnps.ucsd.edu), an open-access knowledge base for community-wide organization and sharing of raw, processed or identified tandem mass (MS/MS) spectrometry data. In GNPS, crowdsourced curation of freely available community-wide reference MS libraries will underpin improved annotations. Data-driven social-networking should facilitate identification of spectra and foster collaborations. We also introduce the concept of 'living data' through continuous reanalysis of deposited data.

Published
2016

6. Direct detection of fungal siderophores on bats with white-nose syndrome via fluorescence microscopy-guided ambient ionization mass spectrometry.

Author

Mascuch, Samantha J, Moree, Wilna J, Hsu, Cheng-Chih, Turner, Gregory G, Cheng, Tina L, Blehert, David S, Kilpatrick, A Marm, Frick, Winifred F, Meehan, Michael J, Dorrestein, Pieter C, and Gerwick, Lena

Subjects
Animals, Chiroptera, Ascomycota, Siderophores, Microscopy, Fluorescence, Spectrometry, Mass, Secondary Ion, Microscopy, Fluorescence, Spectrometry, Mass, Secondary Ion, General Science & Technology
Abstract

White-nose syndrome (WNS) caused by the pathogenic fungus Pseudogymnoascus destructans is decimating the populations of several hibernating North American bat species. Little is known about the molecular interplay between pathogen and host in this disease. Fluorescence microscopy ambient ionization mass spectrometry was used to generate metabolic profiles from the wings of both healthy and diseased bats of the genus Myotis. Fungal siderophores, molecules that scavenge iron from the environment, were detected on the wings of bats with WNS, but not on healthy bats. This work is among the first examples in which microbial molecules are directly detected from an infected host and highlights the ability of atmospheric ionization methodologies to provide direct molecular insight into infection.

Published
2015

10. Parallel Deployment of Passive and Automated Samplers in a University Campus for Surveillance and Variant Profiling of SARS-CoV-2 in Sewage Reveals the Usefulness of the Former

Author

Cha, Gyuhyon, primary, Graham, Katherine E., additional, Zhu, Kevin J., additional, Rao, Gouthami, additional, Lindner, Blake, additional, Kocaman, Kumru, additional, Woo, Seongwook, additional, D'amico, Isabelle, additional, Bingham, Lilia R., additional, Fischer, Jamie M., additional, Flores, Camryn I., additional, Spencer, John W., additional, Yathiraj, Pranav, additional, Chung, Hayong, additional, Biliya, Shweta, additional, Djeddar, Naima, additional, Burton, Liza J., additional, Mascuch, Samantha J., additional, Brown, Joe, additional, Bryksin, Anton, additional, Pinto, Ameet, additional, Hatt, Janet K., additional, and Konstantinidis, Konstantinos T., additional

Published
2022
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13. Mechanism of Action of the Cyanobacterial Marine Natural Product Honaucin A

Author

Mascuch, Samantha J.

Subjects
Academic Dissertations, UCSD Dissertations, Academic Marine biology (Discipline)
Abstract

The bioactive natural products of cyanobacteria show great therapeutic promise in the treatment of a number of ailments. Advancement of these compounds into clinical settings is greatly facilitated by knowledge of the molecules' cellular target and mechanism of action. This thesis presents the multidisciplinary examination of the bioactivity and mechanism of action of the cyanobacterial marine natural product honaucin A. Preliminary evidence for the anti-inflammatory activity of honaucin A was observed in in vitro assays. Evaluation of analogues of honaucin A revealed that certain structural features were necessary for this bioactivity. The marine natural product was subsequently shown to exhibit in vivo activity in the Mouse Ear Edema Model. RNA sequencing of was utilized as a method of understanding differences in gene expression of cultured macrophages that result from honaucin A treatment. This analysis led us to hypothesize that honaucin A exerts its bioactivity through activation of the cytoprotective Nrf2 (nuclear erythroid 2-related factor 2)-ARE/EpRE (antioxidant response element/ electrophile response element) signaling pathway. Activation of this pathway by honaucin A in cultured human cells was confirmed using an Nrf2 luciferase reporter assay. In vitro alkylation experiments with the natural product and N-acetyl-L-cysteine suggested that honaucin A may have the ability to activate this pathway through covalent interaction with the sulfhydryl residues of the repressor protein Keap1. Screening of a library of marine natural products uncovered eight compounds in addition to honaucin A that activate Nrf2-ARE/EpRE. These molecules, isolated from cyanobacteria and an alga, bear structural similarities to honaucin A. This panel of Nrf2-ARE activators may have applications in the treatment of disorders with inflammatory components including neurodegeneration, cadiovascular diseases, cancer, and obesity

Published
2015

14. A blueprint for academic labs to produce SARS-CoV-2 RT-qPCR test kits

Author

Mascuch, Samantha J., primary, Fakhretaha-Aval, Sara, additional, Bowman, Jessica C., additional, Ma, Minh Thu H., additional, Thomas, Gwendell, additional, Bommarius, Bettina, additional, Ito, Chieri, additional, Zhao, Liangjun, additional, Newnam, Gary P., additional, Matange, Kavita R., additional, Thapa, Hem R., additional, Barlow, Brett, additional, Donegan, Rebecca K., additional, Nguyen, Nguyet A., additional, Saccuzzo, Emily G., additional, Obianyor, Chiamaka T., additional, Karunakaran, Suneesh C., additional, Pollet, Pamela, additional, Rothschild-Mancinelli, Brooke, additional, Mestre-Fos, Santi, additional, Guth-Metzler, Rebecca, additional, Bryksin, Anton V., additional, Petrov, Anton S., additional, Hazell, Mallory, additional, Ibberson, Carolyn B., additional, Penev, Petar I., additional, Mannino, Robert G., additional, Lam, Wilbur A., additional, Garcia, Andrés J., additional, Kubanek, Julia M., additional, Agarwal, Vinayak, additional, Hud, Nicholas V., additional, Glass, Jennifer B., additional, Williams, Loren Dean, additional, and Lieberman, Raquel L., additional

Published
2020
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18. Marine Natural Product Honaucin A Attenuates Inflammation by Activating the Nrf2-ARE Pathway

Author

Mascuch, Samantha J., primary, Boudreau, Paul D., additional, Carland, Tristan M., additional, Pierce, N. Tessa, additional, Olson, Joshua, additional, Hensler, Mary E., additional, Choi, Hyukjae, additional, Campanale, Joseph, additional, Hamdoun, Amro, additional, Nizet, Victor, additional, Gerwick, William H., additional, Gaasterland, Teresa, additional, and Gerwick, Lena, additional

Published
2017
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19. Real-Time Metabolomics on Living Microorganisms Using Ambient Electrospray Ionization Flow-Probe

Author

Hsu, Cheng-Chih, primary, ElNaggar, Mariam S., additional, Peng, Yao, additional, Fang, Jinshu, additional, Sanchez, Laura M., additional, Mascuch, Samantha J., additional, Møller, Kirsten A., additional, Alazzeh, Emad K., additional, Pikula, Jiri, additional, Quinn, Robert A., additional, Zeng, Yi, additional, Wolfe, Benjamin E., additional, Dutton, Rachel J., additional, Gerwick, Lena, additional, Zhang, Lixin, additional, Liu, Xueting, additional, Månsson, Maria, additional, and Dorrestein, Pieter C., additional

Published
2013
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22. Recombinant Group B StreptococcusAlpha-Like Protein 3 Is an Effective Immunogen and Carrier Protein

Author

Yang, Hsiao-Hui, Mascuch, Samantha J., Madoff, Lawrence C., and Paoletti, Lawrence C.

Abstract

ABSTRACTConjugate vaccines against pathogens of multiple serotypes are optimized when all components induce functional antibody, resulting in broadened coverage. While most clinical studies of vaccines against group B Streptococcus(GBS) have evaluated conjugates composed of capsular polysaccharide (CPS) coupled to tetanus toxoid, conjugates prepared with GBS proteins as carriers have also been efficacious in animals. Here, we report that recombinant GBS alpha-like protein 3 (rAlp3) is both a strong immunogen and a viable carrier protein for type III CPS. The type III CPS-specific immunoglobulin G (IgG) titer rose from <100 to 64,000 among mice that received type III CPS coupled to rAlp3 (III-rAlp3) compared with an absence of a specific response among mice that received an uncoupled mixture. Most (94%) newborn pups born to III-rAlp-vaccinated dams survived challenge with viable type III GBS, compared with 43% survival among those born to dams that received the uncoupled mixture (P< 0.0001). A tricomponent conjugate of type III CPS, rAlp3, and a GBS recombinant beta C protein lacking its IgA binding site (III-rAlp3-rBCP?IgA) provided protection against a serotype III strain and a serotype Ia strain bearing beta C protein. High-titered anti-rAlp3 rabbit serum opsonized Alp3-containing strains of two GBS serotypes (types V and VIII) and invasive type III strains bearing the cross-reactive Rib protein for in vitro killing by human peripheral blood leukocytes. Thus, the potential exists for the inclusion of rAlp3 in a GBS vaccine formulated to provide multiserotype coverage.

Published
2008
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23. Sharing and community curation of mass spectrometry data with Global Natural Products Social Molecular Networking

Author

Waters, Katrina M., Peng, Yao, McPhail, Kerry L., Zhang, Lixin, Dorrestein, Kathleen, Shi, Wenyuan, Allard, Pierre-Marie, Gerwick, Lena, Torres-Mendoza, Daniel, Pogliano, Kit, Moore, Bradley S., Houson, Hailey, Kleigrewe, Karin, Kyle, Jennifer E., Hsu, Cheng-Chih, Silva, Denise B., Boya, Cristopher A.P., Litaudon, Marc, Charusanti, Pep, Pace, Laura A., Sedio, Brian E., Shinn, Paul, Liu, Xueting, Sims, Amy C., Pevzner, Pavel, Gurr, Joshua, Aigle, Bertrand, Pociute, Egle, Rodríguez, Andrés M. C., Gavilan, Ronnie G., Metz, Thomas O., Maansson, Maria, Quinn, Robert A., Palsson, Bernhard Ø, Dai, Jingqui, Carlson, Erin E., Crüsemann, Max, Ryffel, Florian, Vuong, Lisa, Nothias, Louis-Felix, Linington, Roger G., Alexandrov, Theodore, Phelan, Vanessa V., Kharbush, Jenan J., Zhang, Chen, Müller, Rolf, Lopes, Norberto P., Phapale, Prasad, Bouslimani, Amina, Dorrestein, Pieter C., Yang, Yu-Liang, Engene, Niclas, Elfeki, Maryam, McLean, Jeffrey, Neupane, Ram, O'Neill, Ellis C., Murphy, Brian T., Garg, Neha, Almaliti, Jehad, Helfrich, Eric J. N., Dutton, Rachel J., Wolfender, Jean-Luc, Melnik, Alexey V., Marques, Lucas M., Jelsbak, Lars, Baric, Ralph, Koyama, Nobuhiro, Nielsen, Kristian Fog, Johnson, Andrew R., Klitgaard, Andreas, Esquenazi, Eduardo, Duggan, Brendan M., Northen, Trent, Wang, Mingxun, Boudreau, Paul D., Gutiérrez, Marcelino, Williams, Philip G., Edlund, Anna, Michelsen, Charlotte F., Demarque, Daniel P., Mascuch, Samantha J., Mohimani, Hosein, Lamsa, Anne, Tomasi, Sophie, Glukhov, Evgenia, Larson, Charles B., Macherla, Venkat, Sandoval-Calderón, Mario, Jensen, Paul R., Gerwick, William H., Nguyen, Dac-Trung, Jadhav, Ajit, Meehan, Michael J., Porto, Carla, Parrot, Delphine, Sidebottom, Ashley M., Peryea, Tyler, Floros, Dimitrios J., Vining, Oliver B., Hoffman, Thomas, Sanchez, Laura M., Gonzalez, David J., VanLeer, Danielle, Vorholt, Julia A., Briand, Enora, Sohlenkamp, Christian, Watrous, Jeramie, Kurita, Kenji L., Kersten, Roland D., Knight, Rob, Granatosky, Eve A., Luzzatto-Knaan, Tal, Keyzers, Robert A., Jenkins, Stefan, Liu, Wei-Ting, Traxler, Matthew F., Silva, Ricardo R., Piel, Jörn, Duncan, Katherine R., Kapono, Clifford A., Humpf, Hans-Ulrich, Nguyen, Don Duy, Bandeira, Nuno, Carver, Jeremy J., Liaw, Chih-Chuang, Agarwal, Vinayak, and Zeng, Yi

Subjects
3. Good health
Abstract

The potential of the diverse chemistries present in natural products (NP) for biotechnology and medicine remains untapped because NP databases are not searchable with raw data and the NP community has no way to share data other than in published papers. Although mass spectrometry techniques are well-suited to high-throughput characterization of natural products, there is a pressing need for an infrastructure to enable sharing and curation of data. We present Global Natural Products Social molecular networking (GNPS, http://gnps.ucsd.edu), an open-access knowledge base for community wide organization and sharing of raw, processed or identified tandem mass (MS/MS) spectrometry data. In GNPS crowdsourced curation of freely available community-wide reference MS libraries will underpin improved annotations. Data-driven social-networking should facilitate identification of spectra and foster collaborations. We also introduce the concept of ‘living data’ through continuous reanalysis of deposited data.

24. A blueprint for academic labs to produce SARS-CoV-2 RT-qPCR test kits.

Author

Mascuch SJ, Fakhretaha-Aval S, Bowman JC, Ma MTH, Thomas G, Bommarius B, Ito C, Zhao L, Newnam GP, Matange KR, Thapa HR, Barlow B, Donegan RK, Nguyen NA, Saccuzzo EG, Obianyor CT, Karunakaran SC, Pollet P, Rothschild-Mancinelli B, Mestre-Fos S, Guth-Metzler R, Bryksin AV, Petrov AS, Hazell M, Ibberson CB, Penev PI, Mannino RG, Lam WA, Garcia AJ, Kubanek JM, Agarwal V, Hud NV, Glass JB, Williams LD, and Lieberman RL

Abstract

Widespread testing for the presence of the novel coronavirus SARS-CoV-2 in individuals remains vital for controlling the COVID-19 pandemic prior to the advent of an effective treatment. Challenges in testing can be traced to an initial shortage of supplies, expertise and/or instrumentation necessary to detect the virus by quantitative reverse transcription polymerase chain reaction (RT-qPCR), the most robust, sensitive, and specific assay currently available. Here we show that academic biochemistry and molecular biology laboratories equipped with appropriate expertise and infrastructure can replicate commercially available SARS-CoV-2 RT-qPCR test kits and backfill pipeline shortages. The Georgia Tech COVID-19 Test Kit Support Group, composed of faculty, staff, and trainees across the biotechnology quad at Georgia Institute of Technology, synthesized multiplexed primers and probes and formulated a master mix composed of enzymes and proteins produced in-house. Our in-house kit compares favorably to a commercial product used for diagnostic testing. We also developed an environmental testing protocol to readily monitor surfaces across various campus laboratories for the presence of SARS-CoV-2. Our blueprint should be readily reproducible by research teams at other institutions, and our protocols may be modified and adapted to enable SARS-CoV-2 detection in more resource-limited settings., Competing Interests: Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article.

Published
2020
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