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Background and Aims Hybridization is the main driver of plant diversification, and gene flow via hybridization has multifaceted effects on plant evolution. Carex angustisquama is an extremophyte that grows on soils heavily acidified by volcanism. Despite its habitat distinct from that of other species, this species is known to form interspecific hybrids, implying interspecific gene flow. It is crucial to verify the extent and direction of interspecific gene flow between C. angustisquama and closely related species to understand the evolutionary process of an extremophyte in solfatara fields. Methods In this study, expressed sequence tag–simple sequence repeat markers were utilized to infer the extent and direction of interspecific gene flow between C. angustisquama and closely related species. Key Results Bayesian clustering and simulation analyses revealed that all individuals of the three hybrid species were classified into the first hybrid generation or first backcross to C. angustisquama; therefore, current interspecific gene flow is limited. Moreover, in the Bayesian inference of historical gene flow based on multispecies samples, the model that assumed no interspecific gene flow was the most strongly supported across all species pairs, including phylogenetically close but ecologically distinctive species pairs. Conclusions Our results revealed that interspecific gene flow between C. angustisquama and its related species has been limited both currently and historically. Moreover, our results of Bayesian inference of historical gene flow indicated that extrinsic, rather than intrinsic, factors probably act as isolating barriers between Carex species, with hybrid breakdown via microhabitat segregation being the probable potential barrier. Overall, our findings provide insights into the evolutionary process of an extremophyte in solfatara fields and offer an important example of the mechanisms of diversification of the speciose genus Carex.

How genetic diversity is maintained within a range of species is one of the crucial pieces of information in species conservation. Although the classical central-marginal hypothesis assumes genetic paucity in peripheral populations, this pattern is not always the case. The semi-shrub Chimaphila umbellata subsp. umbellate, population of which are located in the southernmost part of the subspecies distribution, is considered to be a threatened species in Japan. Thus, this study aimed to examine which populations should be preferentially conserved and if the central-marginal hypothesis can be applied to this case. Genetic diversity was examined in 15 populations of C. umbellata subsp. umbellata in Japan using 16 nuclear simple sequence repeat markers. Overall, the genetic diversity values within the populations did not correlate with the latitude of the population locality, although those of the southern marginal populations tended to be lower than those of populations in other regions. While the populations are genetically differentiated from each other, recent population size declines were only detected in a few cases. Bayesian inference of population structure revealed three genetically distinct groups. An approximate Bayesian computation revealed that these three genetic groups were derived from the ancient population in a recent times. The contribution of each population to total genetic diversity was estimated by removing a given population and recalculating the total genetic diversity. Only one population contributed to both gene diversity and allelic diversity, while several populations contributed to one or the other. Considering genetic diversity and structure, the conservation priority of Japanese populations of C. umbellata to preserve genetic diversity is discussed.

Symplocarpus koreanus (Araceae; Orontioideae) from Korea is described as new. Symplocarpus koreanus has long been considered to be conspecific with S. renifolius in Japan, but phylogenetic, morphological, and cytological data indicate its taxonomic distinction. Compared to S. renifolius, S. koreanus has a much smaller spathe and more spherical spadix with fewer, smaller flowers. Previous phylogenetic studies also suggested that S. koreanus is more closely related to nonthermogenic S. nipponicus than to S. renifolius in Japan. Like its nonthermogenic sister species, S. nipponicus, in Korea and Japan, S. koreanus is diploid (2n = 2x = 30), while S. renifolius in Japan is tetraploid (2n = 4x = 60). A detailed species description, geographical distribution, major morphological differences between the species and a dichotomous key to the species in eastern Asia are provided.

Understanding adaptation mechanisms is important in evolutionary biology. Parallel adaptation provides good opportunities to investigate adaptive evolution. To confirm parallel adaptation, it is effective to examine whether the phenotypic similarity has one or multiple origins and to use demographic modeling to consider the gene flow between ecotypes. Solidago yokusaiana is a rheophyte endemic to the Japanese Archipelago that diverged from Solidago virgaurea. This study examined the parallel origins of S. yokusaiana by distinguishing between multiple and single origins and subsequent gene flow. The haplotypes of noncoding chloroplast DNA and genotypes at 14 nuclear simple sequence repeat (nSSR) loci and single-nucleotide polymorphisms (SNPs) revealed by double-digest restriction-associated DNA sequencing (ddRADseq) were used for phylogeographic analysis; the SNPs were also used to model population demographics. Some chloroplast haplotypes were common to S. yokusaiana and its ancestor S. virgaurea. Also, the population genetic structures revealed by nSSR and SNPs did not correspond to the taxonomic species. The demographic modeling supported the multiple origins of S. yokusaiana in at least four districts and rejected a single origin with ongoing gene flow between the two species, implying that S. yokusaiana independently and repeatedly adapted to frequently flooding riversides.

Scrophularia takesimensis is a critically endangered endemic species of Ulleung Island, Korea. A previous molecular phylogenetic study based on nuclear ribosomal DNA (nrDNA) internal transcribed spacer (ITS) sequences with very limited sampling suggested that it is most closely related to the clade comprising S. alata and S. grayanoides. To determine the origin of S. takesimensis, we sampled a total of 171 accessions including S. takesimensis (9 populations and 63 individuals) and two closely related species, S. alata (11 populations and 68 individuals) and S. grayanoides (5 populations and 40 individuals) from eastern Asia and sequenced ITS and two chloroplast DNA (cpDNA) non-coding regions. Previously sequenced representative species of Scrophularia (109 taxa for ITS and 80 taxa for cpDNA) were combined with our data set and analyzed. While the global scale ITS phylogenetic tree suggests monophyly for each of the three eastern Asian species, S. takesimensis appears to be more closely related (albeit weakly) to a clade containing eastern North American/Caribbean species than to either S. alata or S. grayanoides. By contrast, the global scale cpDNA phylogenetic tree demonstrates that the eastern North America/Caribbean clade is sister to a clade comprising the three eastern Asian species. In addition, the monophyletic S. takesimensis is deeply embedded within paraphyletic S. alata, sharing its most recent common ancestor with populations from Japan/Sakhalin. Two divergent, geographically structured cp haplotype groups within S. takesimensis suggest at least two independent introductions from different source areas. A new and accurate chromosome number of S. takesimensis (2n = 94) is reported and some conservation strategies are discussed.

Understanding the causes of the Sino‐Japanese disjunctions in plant taxa has been a central question in eastern Asian biogeography, with vicariance or long‐distance dispersal often invoked to explain such patterns. Diabelia Landrein (Caprifoliaceae; Linnaeoideae) comprises four shrubby species with a Sino‐Japanese disjunct distribution. The species diversification time within Diabelia, covering a long geological history of the formation process of the Sino‐Japanese flora, dated back to the middle Oligocene, therefore, Diabelia would be an ideal model to elucidate the biogeographic patterns of Sino‐Japanese disjunctions with climate fluctuation. In this study, we analyzed complete plastome sequence data for 28 individuals representing all four species of Diabelia. These 28 plastomes were found to be highly similar in overall size (156 243–157 578 bp), structure, gene order, and content. Our phylogenomic analysis of the plastomes supported a close relationship between Diabelia ionostachya (Nakai) Landrein & R.L. Barrett var. wenzhouensis (S.L. Zhou ex Landrein) Landrein from eastern China and Diabelia spathulata (Siebold & Zucc.) Landrein var. spathulata from Japan. Diabelia serrata (Siebold & Zucc.) Landrein was identified as sister to a population of Diabelia sanguinea (Makino) Landrein from Tochigi in central Japan and D. spathulata Landrein, from Toyama, central Japan. Most Diabelia lineages were estimated to have differentiated 8–28 Mya. Our results indicate that two independent vicariance events could explain the disjunction between Japan and Korea in the mid to late Miocene, and between Zhejiang and Japan in the early Miocene.

Edaphic specialization is one of the main drivers of plant diversification and has multifaceted effects on population dynamics. Carex angustisquama is a sedge plant growing only on heavily acidified soil in solfatara fields, where only extremophytes can survive. Because of the lack of closely related species in similar habitats and its disjunct distribution, the species offers ideal settings to investigate the effects of adaptation to solfatara fields and of historical biogeography on the genetic consequences of plant edaphic specialization to solfatara fields. Here, genome-wide single nucleotide polymorphisms were used to reveal the phylogenetic origin of C. angustisquama, and 16 expressed sequence tag-simple sequence repeat markers were employed to infer population demography of C angustisquama. Molecular phylogenetic analysis strongly indicated that C. angustisquama formed a monophyletic clade with Carex doenitzii, a species growing on nonacidified soil in the sympatric subalpine zone. The result of population genetic analysis showed that C. angustisquama has much lower genetic diversity than the sister species, and notably, all 16 loci were completely homozygous in most individuals of C. angustisquama. Approximate Bayesian computation analysis supported the model that assumed hierarchical declines of population size through its evolutionary sequence. We propose that the edaphic specialist in solfatara fields has newly attained the adaptation to solfatara fields in the process of speciation. Furthermore, we found evidence of a drastic reduction in genetic diversity in C. angustisquama, suggesting that the repeated founder effects associated with edaphic specialization and subsequent population demography lead to the loss of genetic diversity of this extremophyte in solfatara fields.

Of the two major speciation modes of endemic plants on oceanic islands, cladogenesis and anagenesis, the latter has been recently emphasized as an effective mechanism for increasing plant diversity in isolated, ecologically homogeneous insular settings. As the only flowering cherry occurring on Ulleung Island in the East Sea (concurrently known as Sea of Japan), Prunus takesimensis Nakai has been presumed to be derived through anagenetic speciation on the island. Based on morphological similarities, P. sargentii Rehder distributed in adjacent continental areas and islands has been suggested as a purported continental progenitor. However, the overall genetic complexity and resultant non-monophyly of closely related flowering cherries have hindered the determination of their phylogenetic relationships as well as the establishment of concrete continental progenitors and insular derivative relationships. Based on extensive sampling of wild flowering cherries, including P. takesimensis and P. sargentii from Ulleung Island and its adjacent areas, the current study revealed the origin and evolution of P. takesimensis using multiple molecular markers. The results of phylogenetic reconstruction and population genetic structure analyses based on single nucleotide polymorphisms detected by multiplexed inter-simple sequence repeat genotyping by sequencing (MIG-seq) and complementary cpDNA haplotypes provided evidence for (1) the monophyly of P. takesimensis; (2) clear genetic differentiation between P. takesimensis (insular derivative) and P. sargentii (continental progenitor); (3) uncertain geographic origin of P. takesimensis, but highly likely via single colonization from the source population of P. sargentii in the Korean Peninsula; (4) no significant reduction in genetic diversity in anagenetically derived insular species, i.e., P. takesimensis, compared to its continental progenitor P. sargentii; (5) no strong population genetic structuring or geographical patterns in the insular derivative species; and (6) MIG-seq method as an effective tool to elucidate the complex evolutionary history of plant groups.

The Tokyo Metropolitan Area Convection Study for Extreme Weather Resilient Cities (TOMACS) began as a Japanese domestic research project in 2010 and aimed to elucidate the mechanisms behind local high-impact weather (LHIW) in urban areas, to improve forecasting techniques for LHIW, and to provide high-resolution weather information to end-users (local governments, private companies, and the general public) through social experiments. Since 2013, the project has been expanded as an international Research and Development Project (RDP) of the World Weather Research Programme (WWRP) of the World Meteorological Organization (WMO). Through this project, the following results were obtained: 1) observation data for LHIW around Tokyo were recorded using a dense network of X-band radars, a C-band polarimetric radar, a Ku-band fast-scanning radar, coherent Doppler lidars, and the Global Navigation Satellite System; 2) quantitative precipitation estimation algorithms for X-band polarimetric radars have been developed as part of an international collaboration; 3) convection initiation by the interaction of sea breezes and urban impacts on the occurrence of heavy precipitation around Tokyo were elucidated by a dense observation network, high-resolution numerical simulations, and different urban surface models; 4) an “imminent” nowcast system based on the vertically integrated liquid water derived from the X-band polarimetric radar network has been developed; 5) assimilation methods for data from advanced observation instruments such as coherent Doppler lidars and polarimetric radars were developed; and 6) public use of high-resolution radar data were promoted through the social experiments.

In this study, we propose a new methodology for analysis of the initial stage of localized convective precipitation. The developed algorithm was used to create three-dimensional constant-altitude plan-position-indicator (3D CAPPI) data, which are high-spatiotemporal-resolution volumetric data, using two X-band polarimetric radars that are located in the Kanto region of Japan. Advection vectors are estimated by applying the normalized cross-correlation method to observed successive precipitation echoes on the polar coordinate system at each antenna tilt angle. The estimated advection vectors are expressed by a linear regression model that depends on time and tilt angle. In addition, 3D CAPPI data utilize the mosaic method to obtain further precipitation information from radar observations. The algorithm produces the detailed 3D structure of rapidly developing convective cells and provides quantitative information on convective precipitation, such as the echo top height, maximum reflectivity, and appearance time and height of each cell. The 3D CAPPI mosaic also clearly shows the back-building process at the initial stage of convective precipitation.

Proto‐Araceae, the earliest diverged lineage within the family Araceae, includes two subfamilies, Gymnostachydoideae (one species) and Orontioideae (eight species). Based on an extensive sampling (a total of 198 accessions) of six chloroplast non‐coding regions (5799 aligned sites), we assessed phylogenetic relationships among the genera and species within subfamily Orontioideae and estimated the timing of intercontinental disjunct events in the Northern Hemisphere. Overall phylogenetic relationships among the genera were consistent with results from previous studies, but several new important findings were discovered, primarily within Symplocarpus Salisb. ex W. P. C. Barton. First, two major lineages within Symplocarpus were identified: one lineage included S. foetidus (L.) Salisb. ex W. Barton, S. nabekuraensis Otsuka & K. Inoue, and S. renifolius Schott ex Tzvelev (Japan), whereas the other included S. nipponicus Makino, S. egorovii N. S. Pavlova & V. A. Nechaev, and S. renifolius (Korea). Symplocarpus renifolius in Japan was tetraploid and closely related to the tetraploid S. foetidus in eastern North America. Populations of S. renifolius in Korea were confirmed to be diploid (2n = 30) and shared the most recent common ancestor with the other diploid species, S. nipponicus. Second, two recently described species, S. nabekuraensis and S. egorovii, were deeply embedded within S. renifolius in Japan and Korea, respectively, and their distinct taxonomic status requires further assessment. Finally, two intercontinental disjunction events in the subfamily, one in Lysichiton Schott between eastern Asia and western North America and the other in Symplocarpus between eastern Asia and eastern North America, were estimated to be between 4.5 and 1.4 million years ago (Pliocene and Pleistocene) and between 1.9 and 0.5 million years ago (Pleistocene), respectively.

This paper presents the major specifications and characteristics of the Ku-band high-speed scanning Doppler radar for volcano observation (KuRAD) introduced to Kagoshima University in March 2017 as well as the results of a test observation at Sakurajima. KuRAD is a Doppler radar for research with a wavelength of approximately 2 cm and uses a 45 cm diameter Luneberg lens antenna as a transmitting and receiving antenna to observe the development of a volcanic eruption column immediately following eruption at a maximum rotation speed of 40 rpm. The maximum transmitter power is 9.6 W and the maximum observational range is 20 km. Observed data includes radar reflectivity factor, Doppler velocity, and Doppler spectrum width. Another feature of KuRAD is an obtained radio station license for observation of a total of seven active volcanos in Kyushu. To assess the basic performance of KuRAD, we carried out test observations of volcanic eruptions at Sakurajima, Kagoshima Prefecture, Japan and collected a total of 87 eruptions (20 of which are explosive eruptions and 7 of which had 3,000 m or higher eruptive smoke from vents). From the eruption data of Showa vent on May 2, 2017, it was confirmed that KuRAD could monitor the three-dimensional internal structure of a volcanic eruption column immediately following eruption. Eruption data from Minamidake of Sakurajima on March 5, 2018, showed that KuRAD successfully observed the eruptive smoke reaching a height of 4,000 m, although the eruptive smoke was covered with clouds and could not be detected by optical instruments of the Japan Meteorological Agency.

A recent phylogenetic study based only on chloroplast DNA (cpDNA) variation revealed that populations of an Isodon species are frequently embedded paraphyletically among other Isodon species. This phylogenetic discrepancy between species taxonomy and molecular phylogeny was considered to have resulted from chloroplast DNA captures and/or incomplete lineage sorting. To elucidate which of these factors was mainly responsible for the observed phylogenetic pattern, we performed phylogenetic analyses of multiple populations of Isodon species in Japan using cpDNA variation, three single‐copy nuclear genes, and double‐digest restriction‐site‐associated DNA sequencing (ddRAD‐seq). Although a species often shared chlorotypes with other species, our phylogenetical analyses based on variation in the three single‐copy nuclear genes and the ddRAD‐seq data showed that most populations belonging to the same species were monophyletic at the species level, suggesting that chloroplast capture may have frequently occurred between Isodon species. Some populations of an intraspecific taxon were embedded paraphyletically within the species, regardless of the large amount of phylogenetic information in nuclear DNA; this incongruity may have resulted from incomplete lineage sorting.

This paper is submitted to accompany the article “Analyses of three-dimensional weather radar data from volcanic eruption clouds” [1] ; it describes three-dimensional (3D) visualizations of the Sakurajima volcanic eruption clouds and the weather radar data used for analyses, as well as their availability and downloading procedures. The radar data were acquired by an operational X-band weather radar located approximately 11 km south of the Showa vent of Sakurajima in Kagoshima, Japan. The original raw radar data are available from the “XRAIN Precipitation Original Data search and Download System”, which is hosted on the website “Data Integration and Analysis System (DIAS)”. Animated images of the radar data shown here, which provide a visual explanation of the temporal evolution and the inner structure of volcanic eruption clouds, were created using the program “Analysis Tools of Three-dimensional Weather Radar Data (AN3D)”. The detailed methods of ANT3D are provided in the co-submitted article “Construction of three-dimensional weather radar data from volcanic eruption clouds” [2] .

[EN] Reviews that summarize the genetic diversity of plant species in relation to their life history and ecological traits show that forest trees have more genetic diversity at population and species levels than annuals or herbaceous perennials. In addition, among-population genetic differentiation is significantly lower in trees than in most herbaceous perennials and annuals. Possible reasons for these differences between trees and herbaceous perennials and annuals have not been discussed critically. Several traits, such as high rates of outcrossing, long-distance pollen and seed dispersal, large effective population sizes (N), arborescent stature, low population density, longevity, overlapping generations, and occurrence in late successional communities, may make trees less sensitive to genetic bottlenecks and more resistant to habitat fragmentation or climate change. We recommend that guidelines for genetic conservation strategies be designed differently for tree species versus other types of plant species. Because most tree species fit an LH scenario (low [L] genetic differentiation and high [H] genetic diversity), tree seeds could be sourced from a few populations distributed across the species’ range. For the in situ conservation of trees, translocation is a viable option to increase N. In contrast, rare herbaceous understory species are frequently HL (high differentiation and low diversity) species. Under the HL scenario, seeds should be taken from many populations with high genetic diversity. In situ conservation efforts for herbaceous plants should focus on protecting habitats because the typically small populations of these species are vulnerable to the loss of genetic diversity. The robust allozyme genetic diversity databases could be used to develop conservation strategies for species lacking genetic information. As a case study of reforestation with several tree species in denuded areas on the Korean Peninsula, we recommend the selection of local genotypes as suitable sources to prevent adverse effects and to insure the successful restoration in the long term., [ES] Las revisiones que resumen la diversidad genética de las plantas en relación con sus características ecológicas y biológicas muestran que los árboles forestales tienen más diversidad genética a nivel de población y de especie que las plantas anuales o las perennes herbáceas. Sumado a esto, la diferenciación genética entre poblaciones es significativamente más baja en los árboles que en la mayoría de las perennes herbáceas y las anuales. Hasta la fecha no se han discutido críticamente las posibles explicaciones de estas diferencias entre los árboles y las perennes herbáceas y las plantas anuales. Varias características, como las tasas altas de alogamia, la dispersión a gran distancia de polen y semillas, el gran tamaño de la población efectiva (Ne), la estatura arbórea, la baja densidad poblacional, la longevidad, el solapamiento de generaciones y la presencia dentro de comunidades sucesionales tardías, pueden generar en los árboles una menor sensibilidad a los cuellos de botella genéticos y una mayor resistencia a la fragmentación del hábitat o al cambio climático. Recomendamos que las directrices para las estrategias de conservación genética estén diseñadas de manera diferente para las especies arbóreas que para otro tipo de plantas. Ya que la mayoría de las especies arbóreas encajan dentro de un escenario LH (baja [L] diferenciación genética y alta [H] diversidad genética), las semillas de los árboles podrían tomarse de unas cuantas poblaciones dispersas a lo largo del área de distribución de la especie. Por lo anterior, para la conservación in situ de los árboles, la translocación es una opción viable para incrementar la Ne. Al contrario de esta situación, las especies herbáceas raras del sotobosque con frecuencia son especies HL (alta diferenciación y baja diversidad). En el escenario HL, las semillas deberían ser recolectadas de muchas poblaciones con diversidad genética alta y los esfuerzos de conservación in situ para las plantas herbáceas deberían enfocarse en la protección del hábitat ya que las poblaciones típicamente pequeñas de estas especies son vulnerables a la pérdida de la diversidad genética. Las robustas bases de datos de diversidad genética aloenzimática podrían usarse para desarrollar estrategias de conservación para las especies que carecen de información genética. Como caso de estudio de reforestación con varias especies arbóreas en áreas deforestadas de la Península de Corea, recomendamos la selección de genotipos locales como fuente adecuada para prevenir los efectos adversos y para asegurar la restauración exitosa a largo plazo., This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT of the Republic of Korea (NRF-2013R1A1A2063524 to M.Y.C. and NRF-2017R1A2B4012215 to M.G.C.), Introduction Higher Genetic Diversity of Trees than Herbaceous Perennials or Annuals Strategies for Conserving Genetic Diversity in Trees and Herbaceous Species Case Study of Restoration of a Denuded Area Conclusions Acknowledgments

Real-time monitoring of volcanic tephra fallout rate is an important factor to predict ash plume dispersion and to mitigate risk to air traffic. Ground-based weather radar has been one of the fundamental instruments to detect the plume and derive eruptive source parameters, such as the tephra fallout rate. The current work presents the use of two small and compact X-band Multi-Parameter (X-MP) radars for a new tephra fallout rate model development and the technical aspects of the system in Sinabung and Merapi Volcanoes. The new model estimates the tephra fallout rate using two radar parameters: the specific differential phase shift parameter and the reflectivity intensity factor. Total cumulated mass estimated from the radar-based tephra fallout rate model from the radar is compared with the plume height model and an empirical radar-based model. A volcanic eruptive index (VEI)-2 of Sinabung generated a plume exceeding 15 km, resulting in a maximum tephra fallout rate of 0.58 kg m−2 h−1 and a total tephra mass of 51 × 106 kg. The VEI 1 of Sinabung caused a plume height of 2.5 km, resulting in a maximum tephra fallout rate of 0.3 kg m−2 h−1 and a total cumulated tephra of 9 × 106 kg. In the last case, a VEI 1 eruption of Mt. Merapi produces a 6 km plume, resulting in a maximum tephra fallout rate of 0.28 kg m−2 h−1 and a total cumulated tephra of 35 × 106 kg. The sector range height indicator scan-mode strategy in the VEI 2 eruption of Mt. Sinabung ran at six degrees azimuth angles capturing only a partial volume of the plume. Thus, the total mass was only 22% of the result from the empirical plume height model, even though the plume height was assumed to be equally the same with the maximum height scanned of radar at 7 km. In contrast, the volumetric scan by a plan position indicator strategy gave a total cumulated tephra mass, that matches better to the result of the empirical plume height model at 65–92%. Based on these results and the ability of the X-MP radar to capture the volcanic plume at the same reported onset time, we can confirm the importance of an X-MP radar for real-time tephra fallout monitoring during an eruption.

Hybridization with alien species can affect the reproduction and persistence of native species. Native Oxalis corniculata and alien O. dillenii are distributed in human-made habitats in Japan, and they coexist with their putative hybrid. We examined the origin of the putative hybrid and the possibility of introgression and reproductive interference between these species by analyzing their morphological, cytological, genetic, and reproductive characteristics. The flower morphology of the putative hybrid was similar to the alien species. The chromosome complement and the amount of nuclear DNA of the putative hybrid were intermediate between the two species. Genotyping of the nuclear ITS region showed the putative hybrid was invariably heterozygous for native and alien alleles. Haplotyping of the chloroplast trnS–trnG region showed that 22 and four hybrids had their maternal origin in the alien and native species, respectively. The ovules of the putative hybrid were sterile. When pollen of the putative hybrid was applied to flowers of the parental species, the fruit set was very low, suggesting that their pollen is only marginally viable. Based on these results, we conclude that the putative hybrid is an F1 hybrid between native and alien Oxalis species. The hybrid is virtually sterile, indicating that hybridization led to a waste of gametes and may result in reproductive interference between the two parental species. The capacity for selfing and/or asexual reproduction may weaken reproductive interference between the species; alternatively, their apparent coexistence may be maintained by dispersal from different parental habitats.

Duchesnea indica (Andrews) Th.Wolf is a species of genus Duchesnea which is nested in Potentilla clade. Its fruit is developed enough to be compared with strawberry, which is a significant key to b...

BACKGROUND AND AIMS: The processes and mechanisms underlying lineage diversification are major topics in evolutionary biology. Eurasian goldenrod species of the Solidago virgaurea complex show remarkable morphological and ecological diversity in the Japanese Archipelago, with ecotypic taxa well adapted to specific environments (climate, edaphic conditions and disturbance regimes). The species complex is a suitable model to investigate the evolutionary processes of actively speciating plant groups, due to its ability to evolve in relation to environmental adaptation and its historical population dynamics. METHODS: Two chloroplast markers, 18 nuclear microsatellite markers and ddRAD-sequencing were used to infer population genetic demography of S. virgaurea complex with its related species/genera. KEY RESULTS: Our analysis showed that populations in Japan form an evolutionary unit, which was genetically diverged from adjacent continental populations. The phylogenetic structure within the archipelago strongly corresponds to the geography, but interestingly there is no concordance between genetic structure and ecotypic boundaries; neighbouring populations of distinct ecotypes share a genetic background. CONCLUSIONS: We propose that the traits specific to the ecotypic entities are maintained by natural selection or are very recently generated and have little effect on the genomes, making genome-wide genetic markers unsuitable for detecting ecotypic differentiation. Furthermore, some sporadically distributed taxa (found as rheophytes and alpine plants) were repeatedly generated from a more widespread taxon in geographically distant areas by means of selection. Overall, this study showed that the goldenrod complex has a high ability to evolve, enabling rapid ecological diversification over a recent timeframe.

South Asian country Nepal characterizes a complex mountain range in this world. The country’s population density is increasing along with rapid growth of population especially over mountainous cities, southern hills and the Terai. On the other hand, a number of fatal natural calamities, such as flash flooding and landslides raised by clutter intensive rainfall, have been increasing since the last decade. To deal with such water hydro meteorological disasters, accurate information on spatial and temporal variation of rainfall distribution is very important. In Nepal, the amount of rainfall has been obtained from limited rain gauge networks, which may leads to many errors in making a Quantitative Precipitation Estimation (QPE). Weather radar observations have recently been highlighted as an alternative option for estimating the spatial and temporal distribution of precipitation across specified time intervals. However, estimating rainfall from radar observation has its own challenges, especially over a mountainous country like Nepal. Another mountainous country Japan is well known for using weather radar observation to make QPE product. Different types of weather radar have been used to record, monitor and forecast precipitation in Japan for both operational and research purposes. A high level research work has also been done on this field. The high spatial and temporal (250-m and 1-min) QPE product obtained from the radar observation is available for the public. It shows good harmony with ground data in the flat and mountain areas of Japan. Though Nepal and Japan are located in different regions, both countries represent complex mountain regions and have been facing natural disaster caused by extreme rainfall. In Nepal, weather radar observation for estimating precipitation amounts has not started on an operational basis till date. Hence, sharing knowledge and skills from Japan’s research on weather radar observation would play a key role to achieve the radar based QPE product in Nepal. Therefore, we discuss about the challenge in obtaining QPE product, considering an example of the progress of weather radar system in Japan. It is believed that any discussion on it will be a reference for weather radar deployment and its QPE product in Nepal in coming days. HYDRO Nepal Journal Journal of Water Energy and Environment Issue: 21, July, 2017 Page: 50-59 Upload Date: July 18, 2017