8 results on '"Masato Hatta"'
Search Results
2. Characterization of a Feline Influenza A(H7N2) Virus.
- Author
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Masato Hatta, Gongxun Zhong, Yuwei Gao, Noriko Nakajima, Shufang Fan, Shiho Chiba, Deering, Kathleen M., Mutsumi Ito, Masaki Imai, Maki Kiso, Sumiho Nakatsu, Lopes, Tiago J., Thompson, Andrew J., McBride, Ryan, Suarez, David L., Macken, Catherine A., Shigeo Sugita, Gabriele Neumann, Hideki Hasegawa, and Paulson, James C.
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INFLUENZA A virus , *ANIMAL shelters , *INFLUENZA transmission , *CAT diseases , *EPIDEMICS - Abstract
During December 2016-February 2017, influenza A viruses of the H7N2 subtype infected ≈500 cats in animal shelters in New York, NY, USA, indicating virus transmission among cats. A veterinarian who treated the animals also became infected with feline influenza A(H7N2) virus and experienced respiratory symptoms. To understand the pathogenicity and transmissibility of these feline H7N2 viruses in mammals, we characterized them in vitro and in vivo. Feline H7N2 subtype viruses replicated in the respiratory organs of mice, ferrets, and cats without causing severe lesions. Direct contact transmission of feline H7N2 subtype viruses was detected in ferrets and cats; in cats, exposed animals were also infected via respiratory droplet transmission. These results suggest that the feline H7N2 subtype viruses could spread among cats and also infect humans. Outbreaks of the feline H7N2 viruses could, therefore, pose a risk to public health. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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3. Amino Acid Changes in the Influenza A Virus PA Protein That Attenuate Avian H5N1 Viruses in Mammals.
- Author
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Shufang Fan, Masato Hatta, Jin Hyun Kim, Mai Quynh Le, Neumann, Gabriele, and Yoshihiro Kawaoka
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AMINO acids , *INFLUENZA A virus , *MAMMAL diseases , *VIRAL tropism , *POLYMERASES , *MICROBIAL virulence , *LABORATORY mice - Abstract
The influenza viral polymerase complex affects host tropism and pathogenicity. In particular, several amino acids in the PB2 polymerase subunit are essential for the efficient replication of avian influenza viruses in mammals. The PA polymerase subunit also contributes to host range and pathogenicity. Here, we report that the PA proteins of several highly pathogenic avian H5N1 viruses have attenuating properties in mammalian cells and that the attenuating phenotype is conferred by strain-specific amino acid changes. Specifically, lysine at position 185 of A/duck/Vietnam/TY165/2010 (TY165; H5N1) PA induced strongly attenuating effects in vitro and in vivo. More importantly, the introduction of the arginine residue commonly found at this position in PA significantly increased the viral polymerase activity of TY165 in mammalian cells and its virulence and pathogenicity in mice. These findings demonstrate that the PA protein plays an important role in influenza virulence and pathogenicity. [ABSTRACT FROM AUTHOR]
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- 2014
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4. Protective Immunity and Persistent Lung Sequelae in Domestic Cats after SARS-CoV-2 Infection.
- Author
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Shiho Chiba, Halfmann, Peter J., Masato Hatta, Tadashi Maemura, Shufang Fan, Armbrust, Tammy, Swartley, Olivia M., Crawford, LaTasha K., and Yoshihiro Kawaoka
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CATS , *SARS-CoV-2 , *DISEASE complications , *COVID-19 , *INFECTION - Abstract
Severe acute respiratory syndrome coronavirus 2 readily transmits between domestic cats. We found that domestic cats that recover from an initial infection might be protected from reinfection. However, we found long-term persistence of inflammation and other lung lesions after infection, despite a lack of clinical symptoms and limited viral replication in the lungs. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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5. Mutations in the NA-like protein of bat influenza H18N11 virus enhance virus replication in mammalian cells, mice, and ferrets.
- Author
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Gongxun Zhong, Shufang Fan, Masato Hatta, Sumiho Nakatsu, Walters, Kevin B., Lopes, Tiago J. S., Wang, Jessica I-Hsuan, Makoto Ozawa, Karasin, Alexander, Yan Li, Suxiang Tong, Donis, Ruben O., Neumann, Gabriele, and Yoshihiro Kawaoka
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INFLUENZA viruses , *FERRET , *VIRAL replication , *BATS , *REVERSE genetics , *TURBINATE bones , *PROXIMAL kidney tubules - Abstract
To characterize bat influenza H18N11 virus, we propagated a reverse genetics generated H18N11 virus in MDCK II cells and detected two adapting mutations in the neuraminidase (NA)-like protein (NA-F144C and NA-T342A, N2 numbering) that increased virus titers in three mammalian cell lines (i.e., Madin-Darby canine kidney, Madin-Darby canine kidney II, and human lung adenocarcinoma Calu-3 cells). In mice, wild-type H18N11 virus replicated only in the lungs of the infected animals, whereas the NA-T342A and NA F144C/T342A mutant viruses were detected in the nasal turbinates in addition to the lungs. Bat influenza viruses have not been tested for their virulence and organ tropism in ferrets. We detected wild-type and single mutant viruses each possessing NA-F144C or NA-T342A in the nasal turbinates of one or several infected ferret(s), respectively. A mutant virus possessing both NA-F144C and T342A was isolated from both the lung and trachea, suggesting broader organ tropism compared with wild-type virus. However, none of the H18N11 viruses caused symptoms in mice or ferrets. The NA-F144C/T342A double mutation did not substantially affect virion morphology or the release of virions from cells. Collectively, our data demonstrate that propagation of bat influenza H18N11 virus in mammalian cells can result in mammalian adapting mutations that could increase virus replicative ability and/or organ tropism; overall, however, these viruses did not replicate to high titers throughout the respiratory tract of mice and ferrets. [ABSTRACT FROM AUTHOR]
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- 2020
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6. Characterization of a new SARS-CoV-2 variant that emerged in Brazil.
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Masaki Imai, Halfmann, Peter J., Seiya Yamayoshi, Kiyoko Iwatsuki-Horimoto, Shiho Chiba, Tokiko Watanabe, Noriko Nakajima, Mutsumi Ito, Makoto Kuroda, Maki Kiso, Tadashi Maemura, Kenta Takahashi, Loeber, Samantha, Masato Hatta, Michiko Koga, Hiroyuki Nagai, Shinya Yamamoto, Makoto Saito, Eisuke Adachi, and Osamu Akasaka
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SARS-CoV-2 , *CONVALESCENT plasma , *GOLDEN hamster , *COVID-19 , *COMMERCIAL products , *MESSENGER RNA - Abstract
The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) plays a key role in viral infectivity. It is also the major antigen stimulating the host's protective immune response, specifically, the production of neutralizing antibodies. Recently, a new variant of SARS-CoV-2 possessing multiple mutations in the S protein, designated P.1, emerged in Brazil. Here, we characterized a P.1 variant isolated in Japan by using Syrian hamsters, a well-established small animal model for the study of SARS-CoV-2 disease (COVID-19). In hamsters, the variant showed replicative abilities and pathogenicity similar to those of early and contemporary strains (i.e., SARS-CoV-2 bearing aspartic acid [D] or glycine [G] at position 614 of the S protein). Sera and/or plasma from convalescent patients and BNT162b2 messenger RNA vaccinees showed comparable neutralization titers across the P.1 variant, S-614D, and S-614G strains. In contrast, the S-614D and S-614G strains were less well recognized than the P.1 variant by serum from a P.1-infected patient. Prior infection with S-614D or S-614G strains efficiently prevented the replication of the P.1 variant in the lower respiratory tract of hamsters upon reinfection. In addition, passive transfer of neutralizing antibodies to hamsters infected with the P.1 variant or the S-614G strain led to reduced virus replication in the lower respiratory tract. However, the effect was less pronounced against the P.1 variant than the S-614G strain. These findings suggest that the P.1 variant may be somewhat antigenically different from the early and contemporary strains of SARS-CoV-2. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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7. Diversity of Influenza A(H5N1) Viruses in Infected Humans, Northern Vietnam, 2004-2010.
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Hirotaka Imai, Dinis, Jorge M., Gongxun Zhong, Moncla, Louise H., Lopes, Tiago J. S., McBride, Ryan, Thompson, Andrew J., Wenjie Peng, Mai thi Q. Le, Hanson, Anthony, Lauck, Michael, Yuko Sakai-Tagawa, Shinya Yamada, Eggenberger, Julie, O'Connor, David H., Suzuki, Yasuo, Masato Hatta, Paulson, James C., Neumann, Gabriele, and Friedrich, Thomas C.
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INFLUENZA A virus, H5N1 subtype , *PATHOGENIC bacteria , *INFECTIOUS disease transmission , *VIRUS diversity , *IMMUNE response , *VIRAL genetics , *BINDING site assay , *POLYMERASE genetics - Abstract
Influenza viruses exist in each host as a collection of genetically diverse variants, which might enhance their adaptive potential. To assess the genetic and functional diversity of highly pathogenic avian influenza A(H5N1) viruses within infected humans, we used deep-sequencing methods to characterize samples obtained from infected patients in northern Vietnam during 2004-2010 on different days after infection, from different anatomic sites, or both. We detected changes in virus genes that affected receptor binding, polymerase activity, or interferon antagonism, suggesting that these factors could play roles in influenza virus adaptation to humans. However, the frequency of most of these mutations remained low in the samples tested, implying that they were not efficiently selected within these hosts. Our data suggest that adaptation of influenza A(H5N1) viruses is probably stepwise and depends on accumulating combinations of mutations that alter function while maintaining fitness. [ABSTRACT FROM AUTHOR]
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- 2018
- Full Text
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8. Mutations in the PA Protein of Avian H5N1 Influenza Viruses Affect Polymerase Activity and Mouse Virulence.
- Author
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Gongxun Zhong, Mai Quynh Le, Lopes, Tiago J. S., Halfmann, Peter, Masato Hatta, Shufang Fan, Neumann, Gabriele, and Yoshihiro Kawaoka
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H5N1 Influenza , *POLYMERASES , *MICROBIAL virulence , *GENETIC mutation , *VIRUS diseases - Abstract
To study the influenza virus determinants of pathogenicity, we characterized two highly pathogenic avian H5N1 influenza viruses isolated in Vietnam in 2012 (A/duck/Vietnam/QT1480/2012 [QT1480]) and 2013 (A/duck/Vietnam/QT1728/2013 [QT1728]) and found that the activity of their polymerase complexes differed significantly, even though both viruses were highly pathogenic in mice. Further studies revealed that the PA-S343A/E347D (PA with the S-to-A change at position 343 and the E-to-D change at position 347) mutations reduced viral polymerase activity and mouse virulence when tested in the genetic background of QT1728 virus. In contrast, the PA-343S/347E mutations increased the polymerase activity of QT1480 and the virulence of a low-pathogenic H5N1 influenza virus. The PA-343S residue (which alone increased viral polymerase activity and mouse virulence significantly relative to viral replication complexes encoding PA-343A) is frequently found in H5N1 influenza viruses of several subclades; infection with a virus possessing this amino acid may pose an increased risk to humans. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
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