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121 results on '"Masamune, Yukito"'

5. A protein kinase similar to MAP kinase activator acts downstream of the Raf kinase in Drosophila

Author

Tsuda, Leo, Inoue, Yoshihiro H., Yoo, Mi-Ae, Mizuno, Masami, Hata, Mami, Lim, Young-Mi, Adachi-Yamada, Takashi, Ryo, Haruko, Masamune, Yukito, and Nishida, Yasuyoshi

Subjects
Drosophila -- Physiological aspects, Protein kinases -- Genetic aspects, Genetic regulation -- Research, Cellular signal transduction -- Research, Biological sciences
Abstract

The Drosophila D-raf gene is involved in multiple signal transduction pathways during cell proliferation, differentiation and development. A new gene downstream of D-raf was identified from screens for D-raf suppressors. Sequence analysis showed that the gene, designated Dsor1, codes for a protein kinase similar to the microtubule-associated protein kinase activator. These results indicate that the signal transduction events mediated by D-raf involve a cascade of phosphorylation reactions.

Published
1993

12. The silencer of mouse Pgk-2 consists of two discrete DNA elements that individually have no effect

Author

Nakanishi Yoshinobu, Masamune Yukito, and Nishiyama Satoshi

Subjects
Male, Phosphoglycerate kinase, Base Sequence, Molecular Sequence Data, Promoter, General Medicine, Biology, Regulatory Sequences, Nucleic Acid, Silencer, Molecular biology, Rats, chemistry.chemical_compound, Mice, Phosphoglycerate Kinase, chemistry, Regulatory sequence, Transcription (biology), Sequence Homology, Nucleic Acid, Gene expression, Testis, Genetics, Animals, Gene, DNA, Cells, Cultured
Abstract

Pgk-2 encodes a testis-specific phosphoglycerate kinase isozyme (PGK), and the expression of mouse Pgk-2 is activated during the spermatogenic pathway at the pachytene spermatocyte stage. We previously reported the identification of a silencer-like cis-acting element in a region between nucleotides (nt) −1404 and −685 of mouse Pgk-2, which could be responsible for the repression of Pgk-2 expression in somatic tissues and pre-meiotic testicular germ cells. In the present study, the silencer was precisely located within an 87-bp region between nt −882 and −796. This region contained two distinct sequences that individually bound factors present in nuclear extracts of mouse cultured cells and rat tissues. The two sequences, when aligned in tandem upstream from the Pgk-2 promoter, inhibited cat expression driven by the promoter in mouse erythroleukemia cells, whereas either sequence alone did not show any effect. The results indicate that the Pgk-2 silencer consists of two distinct DNA elements which do not individually influence promoter activity. Binding of distinct nuclear factors to each DNA element appeared to be required for the silencer action.

Published
1994

21. Novel Drug Delivery System to Bone Using Acidic Oligopeptide: Pharmacokinetic Characteristics and Pharmacological Potential

Author

Sekido, Tohru, primary, Sakura, Naoki, additional, Higashi, Yasuhiko, additional, Miya, Kazuhiro, additional, Nitta, Youko, additional, Nomura, Masaaki, additional, Sawanishi, Hiroyuki, additional, Morito, Keiko, additional, Masamune, Yukito, additional, Kasugai, Shohei, additional, Yokogawa, Koichi, additional, and Miyamoto, Ken-Ich, additional

Published
2001
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42. Multiple Cis-acting DNA elements that regulate transcription of the adenovirus 12 E1A gene.

Author

Yamazaki, Yoshihisa, Shimada, Yuko, Shibata-Sakurai, Hitomi, Masamune, Yukito, and Nakanishi, Yoshinobu

Abstract

To delineate cis-acting elements for adenovirus (Ad) 12 E1A gene transcription, we transfected HeLa and NIH3T3 cells with DNAs having various deletions in the 5′-upstream region linked to the chloramphenicol acetyltransferase gene. Deletions in the regions between nucleotide (nt) positions 54 and 166, and 167 and 200, with respect to the left end of the viral genome at nt position 1, caused a two- to three-fold reduction in transcription. Transcription decreased to an almost undetectable level with loss of the region between nt positions 201 and 282. The effect of these mutations was almost consistent between both cell lines. The region between nt positions 77 and 94 stimulated transcription when situated upstream of the simian virus 40 early promoter in either orientation. Transcription was stimulated about ninefold in the presence of the DNA that encodes the product of the 13S, but not the 12S mRNA of the Ad12 E1A gene. These results indicate that transcription of the Ad12 E1A gene is regulated by multiple cis-acting elements and is stimulated by its own gene product. [ABSTRACT FROM AUTHOR]

Published
1992
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49. Identification of the Region Required for Monomerization of the Rolling Circle Plasmid pKYM.

Author

Yasukawa, Hiroo, Viller, Eric, and Masamune, Yukito

Abstract

Plasmid pKYM, isolated from the Gram-negative bacterium Shigella sonnei, is a small multicopy plasmid which replicates by a rolling circle mechanism. The formation of multimers has been observed in a derivative of pKYM which lost a part of the origin region, and the loss of the monomerization mechanism would have led to these multimers. By analyzing the constructs of several mutants, we discovered that a DNA region required for monomerization was present upstream of the RepK binding site in the replication origin. As either of the T-rich sequence or the inverted repeat sequences which were seen in that region have been lost in the multimer-forming plasmids, these sequences may be necessary for monomerization. [ABSTRACT FROM PUBLISHER]

Published
1998
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50. Rolling-Circle Plasmid pKYM Re-initiates DNA Replication.

Author

Yasukawa, Hiroo and Masamune, Yukito

Abstract

It is believed that rolling-circle plasmids are incapable of re-initiation since they have to maintain their copy number and this is one of the difierences between plasmids and phages as phi-x174. To examine whether a rolling-circle plasmid pKYM is incapable of re-initiating DNA replication, we constructed a plasmid that carries both the pKYM origin (fragment 13, 173 bp) and its truncated origin (fragment 32, 56 bp) in the same orientation. This plasmid yielded two smaller plasmids in the presence of RepK, an initiator protein. We showed that RepK can bind to the fragment 13 but not to fragment 32 which lacks the 3′-moiety of fragment 13. These results imply that RepK initiates DNA replication from fragment 13 and terminates at fragment 32, then the same RepK is used for re-initiation of replication from the fragment 32 region. pKYM is likely to be a unique plasmid that re-initiates DNA replication like a phage phi-x174. [ABSTRACT FROM PUBLISHER]

Published
1997
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