32 results on '"Masami Ijiri"'
Search Results
2. A distinct subpopulation of leukemia initiating cells in acute precursor B lymphoblastic leukemia: quiescent phenotype and unique transcriptomic profile
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Alex Q. Lee, Hiroaki Konishi, Connie Duong, Sakiko Yoshida, Ryan R. Davis, Jonathan E. Van Dyke, Masami Ijiri, Bridget McLaughlin, Kyoungmi Kim, Yueju Li, Laurel Beckett, Nitin Nitin, John D. McPherson, Clifford G. Tepper, and Noriko Satake
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B-ALL ,glucose ,metabolic activity ,leukemia initiating capacity ,leukemia initiating cells ,transcriptome profiling ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
In leukemia, a distinct subpopulation of cancer-initiating cells called leukemia stem cells (LSCs) is believed to drive population expansion and tumor growth. Failing to eliminate LSCs may result in disease relapse regardless of the amount of non-LSCs destroyed. The first step in targeting and eliminating LSCs is to identify and characterize them. Acute precursor B lymphoblastic leukemia (B-ALL) cells derived from patients were incubated with fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1, 3-diazol-4-yl) Amino)-2-Deoxyglucose (NBDG) and sorted based on NBDG uptake. Cell subpopulations defined by glucose uptake were then serially transplanted into mice and evaluated for leukemia initiating capacity. Gene expression profiles of these cells were characterized using RNA-Sequencing (RNA-Seq). A distinct population of NBDG-low cells was identified in patient B-ALL samples. These cells are a small population (1.92% of the entire leukemia population), have lower HLA expression, and are smaller in size (4.0 to 7.0 μm) than the rest of the leukemia population. All mice transplanted with NBDG-low cells developed leukemia between 5 and 14 weeks, while those transplanted with NBDG-high cells did not develop leukemia (p ≤ 0.0001-0.002). Serial transplantation of the NBDG-low mouse model resulted in successful leukemia development. NBDG-medium (NBDG-med) populations also developed leukemia. Interestingly, comprehensive molecular characterization of NBDG-low and NBDG-med cells from patient-derived xenograft (PDX) models using RNA-Seq revealed a distinct profile of 2,162 differentially-expressed transcripts (DETs) (p
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- 2022
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3. Novel Patient Metastatic Pleural Effusion-Derived Xenograft Model of Renal Medullary Carcinoma Demonstrates Therapeutic Efficacy of Sunitinib
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Alex Q. Lee, Masami Ijiri, Ryan Rodriguez, Regina Gandour-Edwards, Joyce Lee, Clifford G. Tepper, Yueju Li, Laurel Beckett, Kit Lam, Neal Goodwin, and Noriko Satake
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renal medullary carcinoma ,sunitinib ,multikinase inhibitor ,patient-derived xenograft model ,metastatic pleural effusion ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
BackgroundRenal medullary carcinoma (RMC) is a rare but aggressive tumor often complicated by early lung metastasis with few treatment options and very poor outcomes. There are currently no verified RMC patient-derived xenograft (PDX) mouse models established from metastatic pleural effusion (PE) available to study RMC and evaluate new therapeutic options.MethodsRenal tumor tissue and malignant PE cells from an RMC patient were successfully engrafted into 20 NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice. We evaluated the histopathological similarity of the renal tumor and PE PDXs with the original patient renal tumor and PE, respectively. We then evaluated the molecular integrity of the renal tumor PDXs between passages, as well as the PE PDX compared to two generations of renal tumor PDXs, by microarray analysis. The therapeutic efficacy of sunitinib and temsirolimus was tested in a serially-transplanted generation of 27 PE PDX mice.ResultsThe pathologic characteristics of the patient renal tumor and patient PE were retained in the PDXs. Gene expression profiling revealed high concordance between the two generations of renal tumor PDXs (RMC-P0 vs. RMC-P1, r=0.865), as well as between the first generation PE PDX and each generation of the renal tumor PDX (PE-P0 vs. RMC-P0, r=0.919 and PE-P0 vs. RMC-P1, r=0.843). A low number (626) of differentially-expressed genes (DEGs) was seen between the first generation PE PDX and the first generation renal tumor PDX. In the PE-P1 xenograft, sunitinib significantly reduced tumor growth (p
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- 2021
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4. The incidence and risk factors of venous thromboembolism in Japanese inpatients with inflammatory bowel disease: a retrospective cohort study
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Katsuyoshi Ando, Mikihiro Fujiya, Yoshiki Nomura, Yuhei Inaba, Yuuya Sugiyama, Takuya Iwama, Masami Ijiri, Keitaro Takahashi, Kazuyuki Tanaka, Aki Sakatani, Nobuhiro Ueno, Shin Kashima, Kentaro Moriichi, Yusuke Mizukami, and Toshikatsu Okumura
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Venous thromboembolism ,Inflammatory bowel disease ,Risk factors ,Medicine ,Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Background/AimsVenous thromboembolism (VTE) is a major extraintestinal manifestation in inflammatory bowel disease (IBD), regarded as an independent risk factor for VTE according to reports from Western countries. However, the incidence and risk factors of VTE in Asian IBD patients are not fully understood. We aimed to reveal the incidence and risk factors of VTE in Japanese IBD inpatients.MethodsThe incidence of VTE in inpatients with IBD (n=340), gastrointestinal cancers (n=557), and other gastrointestinal diseases (n=569) treated at our hospital from 2009 to 2013 was retrospectively investigated. The characteristics and laboratory data of IBD inpatients with and without VTE were compared in univariate and multivariate analyses. Clinical courses of VTE in IBD were surveyed.ResultsVTE was detected in 7.1% of IBD inpatients, significantly higher than in gastrointestinal cancer inpatients (2.5%) and inpatients with other gastrointestinal diseases (0.88%). The incidence of VTE in ulcerative colitis (UC) patients (16.7%) was much higher than that in those with Crohn's disease (3.6%). In the univariate analysis, the risk factors were an older age, central venous catheter, prednisolone, surgery, low serum albumin, high serum C-reactive protein and D-dimer. According to a multivariate analysis, >50 years of age and surgery were the only risk factors. The in-hospital mortality rate of IBD inpatients with VTE was 4.2%.ConclusionsThe incidence of VTE with IBD, especially UC, was found to be high compared with other digestive disease, which was almost equivalent to that of Western countries. The efficacy of prophylaxis needs to be investigated in Asian IBD patients.
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- 2018
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5. Takayasu’s arteritis associated with eosinophilic gastroenteritis, possibly via the overactivation of Th17
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Mikihiro Fujiya, Shin Kashima, Yuya Sugiyama, Takuya Iwama, Masami Ijiri, Kazuyuki Tanaka, Keitaro Takahashi, Katuyoshi Ando, Yoshiki Nomura, Nobuhiro Ueno, Takuma Goto, Kentaro Moriichi, Yusuke Mizukami, Toshikatsu Okumura, Junpei Sasajima, Daisuke Fujishiro, Kensaku Okamoto, and Yuichi Makino
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Takayasu’s arteritis ,Eosinophilic gastroenteritis ,The overactivation of TH17 ,Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Abstract Background Takayasu’s arteritis (TA) is a large-vessel vasculitis pathologically characterized by granulomatous necrotizing vasculitis with giant cells. Although the cause of TA is still unclear, genetic factors as well as immunological abnormalities, particularly the overactivation of Th1 and Th-17, are considered to play important roles in the pathogenesis of this disease. Eosinophilic gastroenteritis (EGE) is a type of refractory inflammation in which numerous eosinophils infiltrate the inflammatory area. It is known that the overactivation of Th2 is associated with the pathogenesis of EGE, although the cause of EGE is still unclear. The immunological abnormalities in TA are therefore thought to be different from those in EGE. To date, no cases of complication of TA and EGE have been reported. Case presentations An 18 year-old female was diagnosed with EGE and treated with prednisolone. At 6 months after completion of the treatment, the patient experienced chest pain, and was diagnosed with TA. TH1 and TH17 immunity are thought to be involved with TA, while TH2 are considered to be involved with EGE. In this case, the expression of IL-17 mRNA in the colon mucosa greatly decreased after prednisolone treatment for EGE. Conclusions This is the first report of TA complicated with EGE, and the overactivation of TH17 is considered to be associated with the pathogenesis of these two diseases.
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- 2018
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6. An elevated expression of serum exosomal microRNA-191, − 21, −451a of pancreatic neoplasm is considered to be efficient diagnostic marker
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Takuma Goto, Mikihiro Fujiya, Hiroaki Konishi, Junpei Sasajima, Shugo Fujibayashi, Akihiro Hayashi, Tatsuya Utsumi, Hiroki Sato, Takuya Iwama, Masami Ijiri, Aki Sakatani, Kazuyuki Tanaka, Yoshiki Nomura, Nobuhiro Ueno, Shin Kashima, Kentaro Moriichi, Yusuke Mizukami, Yutaka Kohgo, and Toshikatsu Okumura
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Pancreatic cancer ,Exosome ,microRNA-21 ,microRNA-451a ,Intraductal papillary mucinous neoplasm ,Tumor marker ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Pancreatic cancer is associated with an extremely poor prognosis, so new biomarkers that can detect the initial stages are urgently needed. The significance of serum microRNA (miR) levels in pancreatic neoplasm such as pancreatic cancer and intraductal papillary mucinous neoplasm (IPMN) diagnosis remains unclear. We herein evaluated the usefulness of miRs enclosed in serum exosomes (ExmiRs) as diagnostic markers. Methods The ExmiRs from patients with pancreatic cancer (n = 32) or IPMN (n = 29), and patients without neoplasms (controls; n = 22) were enriched using ExoQuick-TC™. The expression of ExmiRs was evaluated using a next-generation sequencing analysis, and the selected three miRs through this analysis were confirmed by a quantitative real-time polymerase chain reaction. Results The expression of ExmiR-191, ExmiR-21 and ExmiR-451a was significantly up-regulated in patients with pancreatic cancer and IPMN compared to the controls (p
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- 2018
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7. Ferrichrome identified from induces apoptosis through its iron-binding site in gastric cancer cells
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Masami Ijiri, Mikihiro Fujiya, Hiroaki Konishi, Hiroki Tanaka, Nobuhiro Ueno, Shin Kashima, Kentaro Moriichi, Junpei Sasajima, Katsuya Ikuta, and Toshikatsu Okumura
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Ferrichrome is known to be a siderophore, but it was recently identified as a tumor-suppressive molecule derived from Lactobacillus casei ATCC334 ( L. casei ). In the present study, we investigated the effects of ferrichrome in gastric cancer cells. Cell lines and xenograft models treated with ferrichrome demonstrated growth suppression. The expression levels of cleaved poly (adenosine diphosphate-ribose) polymerase, and cleaved caspase-9 were increased by ferrichrome treatment. Although the tumor-suppressive effects of ferrichrome were almost completely diminished by the iron chelation, the reduction in the intracellular iron by ferrichrome did not correlate with its tumor-suppressive effects. An exhaustive docking simulation indicated that iron-free ferrichrome can make stable conformations with various mammalian molecules, including transporters and receptors. In conclusion, probiotic-derived ferrichrome induced apoptosis in gastric cancer cells. The iron binding site of ferrichrome is the structure responsible for its tumor suppressive function.
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- 2017
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8. Autofluorescence Imaging Reflects the Nuclear Enlargement of Tumor Cells as well as the Cell Proliferation Ability and Aberrant Status of the p53, Ki-67, and p16 Genes in Colon Neoplasms
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Kentaro Moriichi, Mikihiro Fujiya, Yu Kobayashi, Yuki Murakami, Takuya Iwama, Takehito Kunogi, Takahiro Sasaki, Masami Ijiri, Keitaro Takahashi, Kazuyuki Tanaka, Aki Sakatani, Katsuyoshi Ando, Yoshiki Nomura, Nobuhiro Ueno, Shin Kashima, Katsuya Ikuta, Hiroki Tanabe, Yusuke Mizukami, Yusuke Saitoh, and Toshikatsu Okumura
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autofluorescence imaging ,colon neoplasm ,N/C ratio ,methylation ,tumor cell proliferation ,Organic chemistry ,QD241-441 - Abstract
Background: Autofluorescence imaging (AFI) is useful for diagnosing colon neoplasms, but what affects the AFI intensity remains unclear. This study investigated the association between AFI and the histological characteristics, aberrant methylation status, and aberrant expression in colon neoplasms. Methods: Fifty-three patients with colorectal neoplasms who underwent AFI were enrolled. The AFI intensity (F index) was compared with the pathological findings and gene alterations. The F index was calculated using an image analysis software program. The pathological findings were assessed by the tumor crypt density, cell densities, and N/C ratio. The aberrant methylation of p16, E-cadherin, Apc, Runx3, and hMLH1 genes was determined by a methylation-specific polymerase chain reaction. The aberrant expression of p53 and Ki-67 was evaluated by immunohistochemical staining. Results: An increased N/C ratio, the aberrant expression of p53, Ki-67, and the altered methylation of p16 went together with a lower F index. The other pathological findings and the methylation status showed no association with the F index. Conclusions: AFI reflects the nuclear enlargement of tumor cells, the cell proliferation ability, and the altered status of cell proliferation-related genes, indicating that AFI is a useful and practical method for predicting the dysplastic grade of tumor cells and cell proliferation.
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- 2019
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9. Cmpd10357 to treat B-cell acute lymphoblastic leukemia
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Alex Q. Lee, Hiroaki Konishi, Elizabeth Helmke, Masami Ijiri, Jan Michael A. Lerot, Emma Hicks, Jeremy R. Chien, Fredric A. Gorin, and Noriko Satake
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Pediatric ,Pediatric Research Initiative ,Cancer Research ,Tumor ,Childhood Leukemia ,Pediatric Cancer ,Immunology ,Antineoplastic Agents ,Apoptosis ,Cell Biology ,Hematology ,Cardiorespiratory Medicine and Haematology ,Burkitt Lymphoma ,Cell Line ,Mice ,Rare Diseases ,Orphan Drug ,Caspases ,Precursor B-Cell Lymphoblastic Leukemia-Lymphoma ,Genetics ,Animals ,Humans ,Child ,Molecular Biology ,Cancer - Abstract
B-cell acute lymphoblastic leukemia (B-ALL) is the most common type of cancer found in children. Although the overall survival rates are now >80%, 15%-20% of pediatric patients relapse, with survival rates subsequently dropping to 5%-10%. Cmpd10357, 3-amino-5-arylamino-6-chloro-N- (diaminomethylene) pyrazine-2-carboximide, is a highly potent, cell-permeant compound recently shown to have cytotoxic effects on solid tumors, including human breast cancer and high-grade gliomas, independent of their proliferative status. Cmpd10357 demonstrated concentration-dependent cytotoxicity in two human B-ALL cell lines, JM1 and Reh, at half-maximal inhibitory concentrations (IC50) of 3.2 and 3.3 μM, respectively. Cmpd10357, at a dose of 5 mg/kg, significantly prolonged survival in our B-ALL xenograft mouse model, with a median survival time of 49.0 days compared with 45.5 days in the control group (p < 0.05). The cytotoxicity of Cmpd10357 demonstrated caspase-independent, nonapoptotic cancer cell demise associated with the nuclear translocation of apoptosis-inducing factor (AIF). The cytotoxicity of Cmpd10357 in B-ALL cells was inhibited by Necrostatin-1 but not by Necrosulfonamide. These studies suggest that an AIF-mediated, caspase-independent necrosis mechanism of Cmpd10357 in B-ALL could be used in combination with traditional apoptotic chemotherapeutic agents.
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- 2023
10. A distinct subpopulation of leukemia initiating cells in acute precursor B lymphoblastic leukemia: quiescent phenotype and unique transcriptomic profile
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Alex Q, Lee, Hiroaki, Konishi, Connie, Duong, Sakiko, Yoshida, Ryan R, Davis, Jonathan E, Van Dyke, Masami, Ijiri, Bridget, McLaughlin, Kyoungmi, Kim, Yueju, Li, Laurel, Beckett, Nitin, Nitin, John D, McPherson, Clifford G, Tepper, and Noriko, Satake
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Pediatric ,Cancer Research ,Transplantation ,Pediatric Cancer ,Childhood Leukemia ,Human Genome ,Oncology and Carcinogenesis ,B-ALL ,leukemia initiating cells ,transcriptome profiling ,Hematology ,metabolic activity ,Stem Cell Research ,Rare Diseases ,Oncology ,leukemia initiating capacity ,Stem Cell Research - Nonembryonic - Human ,Genetics ,2.1 Biological and endogenous factors ,glucose ,Aetiology ,Cancer ,Biotechnology - Abstract
In leukemia, a distinct subpopulation of cancer-initiating cells called leukemia stem cells (LSCs) is believed to drive population expansion and tumor growth. Failing to eliminate LSCs may result in disease relapse regardless of the amount of non-LSCs destroyed. The first step in targeting and eliminating LSCs is to identify and characterize them. Acute precursor B lymphoblastic leukemia (B-ALL) cells derived from patients were incubated with fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1, 3-diazol-4-yl) Amino)-2-Deoxyglucose (NBDG) and sorted based on NBDG uptake. Cell subpopulations defined by glucose uptake were then serially transplanted into mice and evaluated for leukemia initiating capacity. Gene expression profiles of these cells were characterized using RNA-Sequencing (RNA-Seq). A distinct population of NBDG-low cells was identified in patient B-ALL samples. These cells are a small population (1.92% of the entire leukemia population), have lower HLA expression, and are smaller in size (4.0 to 7.0 μm) than the rest of the leukemia population. All mice transplanted with NBDG-low cells developed leukemia between 5 and 14 weeks, while those transplanted with NBDG-high cells did not develop leukemia (p ≤ 0.0001-0.002). Serial transplantation of the NBDG-low mouse model resulted in successful leukemia development. NBDG-medium (NBDG-med) populations also developed leukemia. Interestingly, comprehensive molecular characterization of NBDG-low and NBDG-med cells from patient-derived xenograft (PDX) models using RNA-Seq revealed a distinct profile of 2,162 differentially-expressed transcripts (DETs) (pin vivo, showing leukemia initiating capacity. Our study provides insight into the biologic mechanisms of B-ALL initiation and survival.
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- 2022
11. The efficacy of the submucosal injection of lidocaine during endoscopic submucosal dissection for colorectal neoplasms: a multicenter randomized controlled study
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Shin Kashima, Yuki Murakami, Keitaro Takahashi, Hiroki Tanabe, Toshikatsu Okumura, Katsuyoshi Ando, Takahiro Sasaki, Yusuke Saito, Takuya Iwama, Nobuhiro Ueno, Masami Ijiri, Mikihiro Fujiya, Kazuyuki Tanaka, and Kentaro Moriichi
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medicine.medical_specialty ,Endoscopic Mucosal Resection ,Lidocaine ,Article ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Randomized controlled trial ,law ,Internal medicine ,Clinical endpoint ,Humans ,Medicine ,Prospective Studies ,Adverse effect ,Prospective cohort study ,Peristalsis ,business.industry ,Dissection ,Hepatology ,Treatment Outcome ,030220 oncology & carcinogenesis ,Anesthesia ,030211 gastroenterology & hepatology ,Surgery ,Colorectal Neoplasms ,business ,Abdominal surgery ,medicine.drug - Abstract
Background Endoscopic submucosal dissection (ESD) is currently a common procedure although it requires a long procedural time. We conducted a prospective study to determine the efficacy and safety of lidocaine injection for shortening the procedural time and relieving bowel peristalsis during ESD. Methods A multicenter randomized controlled study was conducted in three hospitals. Ninety-one patients who underwent colorectal ESD were enrolled. Patients were randomly divided into two groups using the envelope method: the lidocaine group and saline group. The primary endpoint was the procedural time, and the secondary endpoints were the procedural time in each part of the colon and the grade of bowel peristalsis and the incidence and amounts of antispasmodic drugs use and adverse events. Results The patients’ demographics were not markedly different between the two groups. The mean procedural time in the lidocaine group was not markedly different from that in the saline group. In contrast, at the proximal site, the procedural time in the lidocaine group (57 min) was significantly shorter in the saline group (80 min). The grade of bowel peristalsis in the lidocaine group (0.67) was significantly lower than in the saline group (1.17). Antispasmodic drug use was significantly rarer in the lidocaine group than in the saline group. The incidence of adverse events was not markedly different between the two groups. Conclusions Local lidocaine injection is a feasible option for preventing bowel peristalsis, particularly in the proximal colon, leading to a reduced procedural time for ESD and decreased antispasmodic drug use. University Hospital Medical Information Network Center (UMIN number: 000022843).
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- 2020
12. Probiotic-derived ferrichrome inhibits the growth of refractory pancreatic cancer cells
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Takuya Iwama, Mikihiro Fujiya, Takuma Goto, Akemi Kita, Naoki Ogawa, Shuhei Takauji, Masami Ijiri, Toshikatsu Okumura, Katsuyoshi Ando, Hiroki Tanaka, Aki Sakatani, Shin Kashima, Yuki Murakami, Hiroaki Konishi, and Nobuhiro Ueno
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0301 basic medicine ,ferrichrome ,Male ,Cancer Research ,Biology ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Pancreatic cancer ,Cell Line, Tumor ,medicine ,cancer ,Animals ,Humans ,5-FU ,Ferrichrome ,Cell Proliferation ,Cisplatin ,Oncogene ,Probiotics ,Cancer ,Articles ,Cell cycle ,medicine.disease ,Xenograft Model Antitumor Assays ,Pancreatic Neoplasms ,Lacticaseibacillus casei ,030104 developmental biology ,Oncology ,chemistry ,Apoptosis ,Drug Resistance, Neoplasm ,030220 oncology & carcinogenesis ,pancreatic ,Cancer cell ,Injections, Intravenous ,Cancer research ,Fluorouracil ,medicine.drug - Abstract
Pancreatic cancer is associated with a poor prognosis due to challenges in early detection, severe progression of the primary tumor, metastatic lesions, and resistance to antitumor agents. However, previous studies have indicated a relationship between the microbiome and pancreatic cancer outcomes. Our previous study demonstrated that ferrichrome derived from Lactobacillus casei, a probiotic bacteria, exhibited tumor‑suppressive effects in colorectal and gastric cancer, and that the suppressive effects were stronger than conventional antitumor agents, such as 5‑fluorouracil (5‑FU) and cisplatin, suggesting that certain probiotics exert antitumorigenic effects. However, whether or not probiotic‑derived molecules, including ferrichrome, exert a tumor‑suppressive effect in other gastrointestinal tumors, such as pancreatic cancer, remains unclear. In the present study, it was demonstrated that probiotic‑derived ferrichrome inhibited the growth of pancreatic cancer cells, and its tumor‑suppressive effects were further revealed in 5‑FU‑resistant pancreatic cancer cells in vitro and in vivo in a mouse xenograft model. Ferrichrome inhibited the progression of cancer cells via dysregulation of the cell cycle by activating p53. DNA fragmentation and cleavage of poly (ADP‑ribose) polymerase were induced by ferrichrome treatment, suggesting that ferrichrome induced apoptosis in pancreatic cancer cells. A transcriptome analysis revealed that the expression p53‑associated mRNAs was significantly altered by ferrichrome treatment. Thus, the tumor‑suppressive effects of probiotics may mediated by probiotic‑derived molecules, such as ferrichrome, which may have applications as an antitumor drug, even in refractory and 5‑FU‑resistant pancreatic cancer.
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- 2020
13. White coat status is a predictive marker for post-esophageal endoscopic submucosal dissection stricture: a retrospective study
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Takeshi Saito, Katsuyoshi Ando, Masami Ijiri, Toshikatsu Okumura, Mitsuru Goto, Keitaro Takahashi, Yuya Sugiyama, Kazuyuki Tanaka, Takuya Iwama, Takahiro Sasaki, Mikihiro Fujiya, Yoshiki Nomura, Aki Sakatani, Shin Kashima, Yuki Murakami, Nobuhiro Ueno, and Kentaro Moriichi
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Male ,medicine.medical_specialty ,Esophageal Mucosa ,Endoscopic Mucosal Resection ,Constriction, Pathologic ,Gastroenterology ,03 medical and health sciences ,Esophagus ,0302 clinical medicine ,Risk Factors ,Clinical Decision Rules ,Internal medicine ,medicine ,Humans ,In patient ,Endoscopy, Digestive System ,Postoperative Period ,Ulcer ,Esophageal stricture ,Aged ,Retrospective Studies ,Aged, 80 and over ,Predictive marker ,business.industry ,White coat ,Prevention ,Retrospective cohort study ,Endoscopic submucosal dissection ,Odds ratio ,Middle Aged ,medicine.disease ,Cardiothoracic surgery ,Case-Control Studies ,030220 oncology & carcinogenesis ,Esophageal Stenosis ,Original Article ,Female ,Steroids ,030211 gastroenterology & hepatology ,Prediction ,business - Abstract
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creat iveco mmons .org/licen ses/by/4.0/), BACKGROUND: Steroid therapy is primarily used to prevent esophageal stricture after endoscopic submucosal dissection (ESD). However, esophageal stricture can still occur after preventive therapy, and the effect of preventive steroid therapy cannot be predicted before stricture formation. This study aimed to clarify the risk factors for esophageal stricture after preventive steroid therapy. METHODS: This was a retrospective study conducted at three institutions. From January 2011 to February 2018, 28 large-sized SENs in 26 patients who had a mucosal defect that involved more than three-quarters of the esophageal circumference were enrolled. We classified white coats on artificial ulcers after esophageal ESD into three groups (thin, moderately thick, thick) based on endoscopic images obtained on postoperative day 7. RESULTS: The white coat status on the artificial ulcer after ESD was a significant risk factor for post-ESD stricture (p
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- 2019
14. The Incidence and Risk Factors of Venous Thromboembolism in Patients with Inflammatory Bowel Disease: A Prospective Multicenter Cohort Study
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Hiroki Tanabe, Satoshi Yamada, Hiroshi Nakase, Shin Kashima, Katsuyoshi Ando, Kazumi Akasaka, Yu Kobayashi, Nobuhiro Ueno, Toshikatsu Okumura, Yusuke Mizukami, Takuya Iwama, Yoshiki Nomura, Keitaro Takahashi, Kentaro Moriichi, Masami Ijiri, Yuya Sugiyama, Yuhei Inaba, Mikihiro Fujiya, and Satoshi Fujii
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Adult ,Male ,medicine.medical_specialty ,Computed Tomography Angiography ,medicine.medical_treatment ,Population ,Contrast Media ,digestive system ,Inflammatory bowel disease ,Crohn Disease ,Japan ,Risk Factors ,Internal medicine ,medicine ,Humans ,Prospective Studies ,cardiovascular diseases ,Risk factor ,education ,Aged ,Gastrointestinal Neoplasms ,Ultrasonography ,education.field_of_study ,medicine.diagnostic_test ,business.industry ,Incidence ,Incidence (epidemiology) ,Gastroenterology ,Venous Thromboembolism ,Middle Aged ,equipment and supplies ,medicine.disease ,Ulcerative colitis ,digestive system diseases ,Hospitalization ,Colitis, Ulcerative ,Female ,business ,Central venous catheter ,Cohort study ,Partial thromboplastin time - Abstract
Background: Venous thromboembolism (VTE) has been shown to be more frequent in inflammatory bowel disease (IBD) than in the general population in Western studies. However, the actual state of VTE in Asian IBD remains poorly understood. Aims: To reveal the incidence of VTE in IBD patients in Japan. Methods: Eighty-five patients admitted to 3 gastroenterology centers were registered from 2013 to 2018. The incidence of VTE in patients with IBD (n = 42) was prospectively compared to that among patients with other digestive diseases (n = 43). The presence of VTE was surveyed using contrast-enhanced computed tomography and/or ultrasonography at admission and at 1–2 weeks after admission. The patient characteristics and laboratory data of IBD patients with or without VTE were compared to determine the risk factors for VTE. Results: The incidence of VTE with IBD was 16.7%, which was significantly more frequent than with other digestive diseases (2.3%; p = 0.0296). In IBD patients, VTE was detected in 6 of 22 patients with ulcerative colitis (27.2%) but in only 1 of 20 patients with Crohn’s disease (5.0%). VTE was diagnosed at admission in 4 IBD patients and 2 weeks after admission in 3 IBD patients. The risk factors of VTE in IBD were the presence of an indwelling central venous catheter, a low level of total protein, a low activated partial thromboplastin time, and a high level of fibrinogen degradation products. Conclusion: VTE was frequently detected in Japanese IBD patients both at and after admission. Adequate screening and prophylaxis for VTE is deemed necessary in IBD.
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- 2018
15. A distinct subpopulation of leukemia initiating cells in acute precursor B lymphoblastic leukemia: quiescent phenotype and unique transcriptomic profile.
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Lee, Alex Q., Hiroaki Konishi, Duong, Connie, Sakiko Yoshida, Davis, Ryan R., Van Dyke, Jonathan E., Masami Ijiri, McLaughlin, Bridget, Kyoungmi Kim, Yueju Li, Beckett, Laurel, Nitin, Nitin, McPherson, John D., Tepper, Clifford G., and Noriko Satake
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LYMPHOBLASTIC leukemia ,LEUKEMIA ,GENE expression profiling ,TRANSCRIPTOMES ,HIERARCHICAL clustering (Cluster analysis) - Abstract
In leukemia, a distinct subpopulation of cancer-initiating cells called leukemia stem cells (LSCs) is believed to drive population expansion and tumor growth. Failing to eliminate LSCs may result in disease relapse regardless of the amount of non-LSCs destroyed. The first step in targeting and eliminating LSCs is to identify and characterize them. Acute precursor B lymphoblastic leukemia (B-ALL) cells derived from patients were incubated with fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1, 3-diazol-4-yl) Amino)-2-Deoxyglucose (NBDG) and sorted based on NBDG uptake. Cell subpopulations defined by glucose uptake were then serially transplanted into mice and evaluated for leukemia initiating capacity. Gene expression profiles of these cells were characterized using RNA-Sequencing (RNA-Seq). A distinct population of NBDG-low cells was identified in patient B-ALL samples. These cells are a small population (1.92% of the entire leukemia population), have lower HLA expression, and are smaller in size (4.0 to 7.0 mm) than the rest of the leukemia population. All mice transplanted with NBDG-low cells developed leukemia between 5 and 14 weeks, while those transplanted with NBDG-high cells did not develop leukemia (p ≤ 0.0001-0.002). Serial transplantation of the NBDG-low mouse model resulted in successful leukemia development. NBD-Gmedium (NBDG-med) populations also developed leukemia. Interestingly, comprehensive molecular characterization of NBDG-low and NBDG-med cells from patient-derived xenograft (PDX) models using RNA-Seq revealed a distinct profile of 2,162 differentially-expressed transcripts (DETs) (p<0.05) with 70.6% down-regulated in NBDG-low cells. Hierarchical clustering of DETs showed distinct segregation of NBDG-low from NBDG-med and NBDG-high groups with marked transcription expression alterations in the NBDG-low group consistent with cancer survival. In conclusion, A unique subpopulation of cells with low glucose uptake (NBDG-low) in B-ALL was discovered. These cells, despite their quiescence characteristics, once transplanted in mice, showed potent leukemia initiating capacity. Although NBDG-med cells also initiated leukemia, gene expression profiling revealed a distinct signature that clearly distinguishes NBDG-low cells from NBDG-med and the rest of the leukemia populations. These results suggest that NBDG-low cells may represent quiescent LSCs. These cells can be activated in the appropriate environment in vivo, showing leukemia initiating capacity. Our study provides insight into the biologic mechanisms of B-ALL initiation and survival. [ABSTRACT FROM AUTHOR]
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- 2022
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16. A Case of Alpha-Fetoprotein-Producing Adenocarcinoma of the Esophagogastric Junction in which Long-Term Survival Was Achieved by Means of Individualized Multidisciplinary Therapy
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Kazuyuki Tanaka, Kentaro Moriichi, Masami Ijiri, Junpei Sasajima, Yusuke Mizukami, Yoshiki Nomura, Takahiro Ito, Mikihiro Fujiya, Nobuhiro Ueno, Shin Kashima, Toshikatsu Okumura, Keitaro Takahashi, Takuma Goto, Hiroki Tanabe, and Katsuyoshi Ando
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Ablation Techniques ,Male ,medicine.medical_specialty ,Time Factors ,Esophageal Neoplasms ,medicine.medical_treatment ,education ,Case Report ,Adenocarcinoma ,Gastroenterology ,Metastasis ,03 medical and health sciences ,0302 clinical medicine ,Gastrectomy ,Stomach Neoplasms ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Carcinoma ,Humans ,natural sciences ,Lymph node ,Tumor marker ,Aged ,Ethanol ,business.industry ,Liver Neoplasms ,Cancer ,medicine.disease ,Radiation therapy ,Esophagectomy ,medicine.anatomical_structure ,Treatment Outcome ,Oncology ,Chemotherapy, Adjuvant ,030220 oncology & carcinogenesis ,Hepatocellular carcinoma ,Lymph Node Excision ,030211 gastroenterology & hepatology ,Esophagogastric Junction ,alpha-Fetoproteins ,business - Abstract
Carcinoma of the esophagogastric junction (EGJ) is a type of upper digestive cancer that occurs within 2 cm above or below the EGJ regardless of the histological type. Most cases of carcinoma of the EGJ are detected at an advanced stage and thus show a poor prognosis [1, 2]. The most frequent sites of lymph node metastasis in carcinoma of the EGJ differ from carcinomas in other portions of the gastric tract and esophageal cancers, and a thoracic approach is sometimes taken during surgery. For this reason, unusual procedures (including lymph node dissection) and reconstruction methods are frequently required [3–6]. To date, the therapeutic strategy has not been fully established for carcinoma of the EGJ. Alpha-fetoprotein (AFP) is a useful tumor marker for primary hepatocellular carcinoma and yolk sac tumor [7]. Although AFP-producing cancer develops in the stomach, such tumors rarely developed at the EGJ. AFP-producing carcinoma is thought to have a high potential for metastasis, particularly liver metastasis [8–11] and thus show a poor prognosis [10, 12]. We herein report a case of AFP-producing carcinoma of the EGJ in which long-term survival was achieved through multidisciplinary therapy.
- Published
- 2018
17. An elevated expression of serum exosomal microRNA-191, − 21, −451a of pancreatic neoplasm is considered to be efficient diagnostic marker
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Shin Kashima, Hiroaki Konishi, Akihiro Hayashi, Masami Ijiri, Toshikatsu Okumura, Kazuyuki Tanaka, Mikihiro Fujiya, Hiroki Sato, Tatsuya Utsumi, Junpei Sasajima, Yoshiki Nomura, Yusuke Mizukami, Aki Sakatani, Takuma Goto, Takuya Iwama, Shugo Fujibayashi, Yutaka Kohgo, Kentaro Moriichi, and Nobuhiro Ueno
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Male ,0301 basic medicine ,Cancer Research ,medicine.medical_specialty ,学位授与年月日:平成30年6月29日 ,Exosomes ,Gastroenterology ,lcsh:RC254-282 ,Disease-Free Survival ,microRNA-451a ,03 medical and health sciences ,0302 clinical medicine ,Surgical oncology ,Internal medicine ,Pancreatic cancer ,microRNA ,Biomarkers, Tumor ,Genetics ,medicine ,Humans ,Neoplasm ,Tumor marker ,Aged ,Receiver operating characteristic ,Intraductal papillary mucinous neoplasm ,business.industry ,Area under the curve ,Middle Aged ,Prognosis ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Adenocarcinoma, Mucinous ,Gene Expression Regulation, Neoplastic ,Pancreatic Neoplasms ,Exosome ,MicroRNAs ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,旭川医科大学:博士(医学)(乙第473号) ,Female ,business ,Research Article ,Carcinoma, Pancreatic Ductal ,microRNA-21 - Abstract
Background Pancreatic cancer is associated with an extremely poor prognosis, so new biomarkers that can detect the initial stages are urgently needed. The significance of serum microRNA (miR) levels in pancreatic neoplasm such as pancreatic cancer and intraductal papillary mucinous neoplasm (IPMN) diagnosis remains unclear. We herein evaluated the usefulness of miRs enclosed in serum exosomes (ExmiRs) as diagnostic markers. Methods The ExmiRs from patients with pancreatic cancer (n = 32) or IPMN (n = 29), and patients without neoplasms (controls; n = 22) were enriched using ExoQuick-TC™. The expression of ExmiRs was evaluated using a next-generation sequencing analysis, and the selected three miRs through this analysis were confirmed by a quantitative real-time polymerase chain reaction. Results The expression of ExmiR-191, ExmiR-21 and ExmiR-451a was significantly up-regulated in patients with pancreatic cancer and IPMN compared to the controls (p
- Published
- 2018
18. Autofluorescence Imaging Reflects the Nuclear Enlargement of Tumor Cells as well as the Cell Proliferation Ability and Aberrant Status of the p53, Ki-67, and p16 Genes in Colon Neoplasms
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Yusuke Mizukami, Kazuyuki Tanaka, Katsuyoshi Ando, Mikihiro Fujiya, Yoshiki Nomura, Katsuya Ikuta, Hiroki Tanabe, Aki Sakatani, Masami Ijiri, Nobuhiro Ueno, Yusuke Saitoh, Takuya Iwama, Takahiro Sasaki, Kentaro Moriichi, Shin Kashima, Takehito Kunogi, Yuki Murakami, Yu Kobayashi, Toshikatsu Okumura, and Keitaro Takahashi
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Pathology ,medicine.medical_specialty ,Cell ,tumor cell proliferation ,Pharmaceutical Science ,Article ,Analytical Chemistry ,autofluorescence imaging ,lcsh:QD241-441 ,03 medical and health sciences ,0302 clinical medicine ,lcsh:Organic chemistry ,Drug Discovery ,medicine ,Humans ,Physical and Theoretical Chemistry ,Pathological ,Cyclin-Dependent Kinase Inhibitor p16 ,Colonoscopes ,biology ,Cell growth ,Optical Imaging ,Organic Chemistry ,Methylation ,Cadherins ,Immunohistochemistry ,colon neoplasm ,Autofluorescence ,N/C ratio ,Core Binding Factor Alpha 3 Subunit ,Ki-67 Antigen ,medicine.anatomical_structure ,Chemistry (miscellaneous) ,030220 oncology & carcinogenesis ,Ki-67 ,Colonic Neoplasms ,biology.protein ,Colon neoplasm ,Molecular Medicine ,030211 gastroenterology & hepatology ,methylation ,Tumor Suppressor Protein p53 ,MutL Protein Homolog 1 ,Software - Abstract
Background: Autofluorescence imaging (AFI) is useful for diagnosing colon neoplasms, but what affects the AFI intensity remains unclear. This study investigated the association between AFI and the histological characteristics, aberrant methylation status, and aberrant expression in colon neoplasms. Methods: Fifty-three patients with colorectal neoplasms who underwent AFI were enrolled. The AFI intensity (F index) was compared with the pathological findings and gene alterations. The F index was calculated using an image analysis software program. The pathological findings were assessed by the tumor crypt density, cell densities, and N/C ratio. The aberrant methylation of p16, E-cadherin, Apc, Runx3, and hMLH1 genes was determined by a methylation-specific polymerase chain reaction. The aberrant expression of p53 and Ki-67 was evaluated by immunohistochemical staining. Results: An increased N/C ratio, the aberrant expression of p53, Ki-67, and the altered methylation of p16 went together with a lower F index. The other pathological findings and the methylation status showed no association with the F index. Conclusions: AFI reflects the nuclear enlargement of tumor cells, the cell proliferation ability, and the altered status of cell proliferation-related genes, indicating that AFI is a useful and practical method for predicting the dysplastic grade of tumor cells and cell proliferation.
- Published
- 2019
19. Mo1041 PROBIOTIC-DERIVED FERRICHROME INHIBITS THE GROWTH OF REFRACTORY PANCREATIC CANCER CELLS
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Yusuke Mizukami, Katsuyoshi Ando, Hiroki Tanabe, Shuhei Takauji, Shin Kashima, Toshikatsu Okumura, Takuya Iwama, Nobuhiro Ueno, Yuuki Murakami, Hiroaki Konishi, Yuya Suglyama, Hiroki Tanaka, Kentaro Moriichi, Mikihiro Fujiya, Takehito Kunogi, Masami Ijiri, Shotaro Isozaki, Akemi Kita, Keitaro Takahashi, Aki Sakatani, and Takuma Goto
- Subjects
Probiotic ,chemistry.chemical_compound ,Hepatology ,chemistry ,Refractory ,law ,Pancreatic cancer ,Gastroenterology ,medicine ,Cancer research ,medicine.disease ,law.invention ,Ferrichrome - Published
- 2020
20. Takayasu’s arteritis associated with eosinophilic gastroenteritis, possibly via the overactivation of Th17
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Shin Kashima, Kazuyuki Tanaka, Nobuhiro Ueno, Daisuke Fujishiro, Toshikatsu Okumura, Kensaku Okamoto, Yusuke Mizukami, Yuichi Makino, Katuyoshi Ando, Yoshiki Nomura, Takuya Iwama, Kentaro Moriichi, Junpei Sasajima, Mikihiro Fujiya, Masami Ijiri, Yuya Sugiyama, Keitaro Takahashi, and Takuma Goto
- Subjects
Pathology ,medicine.medical_specialty ,020209 energy ,Takayasu's arteritis ,Eosinophilic gastroenteritis ,Case Report ,02 engineering and technology ,Microbiology ,Pathogenesis ,03 medical and health sciences ,0302 clinical medicine ,Virology ,Necrotizing Vasculitis ,0202 electrical engineering, electronic engineering, information engineering ,Medicine ,Arteritis ,lcsh:RC799-869 ,The overactivation of TH17 ,030203 arthritis & rheumatology ,business.industry ,Gastroenterology ,Takayasu’s arteritis ,medicine.disease ,Infectious Diseases ,Giant cell ,Prednisolone ,lcsh:Diseases of the digestive system. Gastroenterology ,Parasitology ,business ,Vasculitis ,medicine.drug - Abstract
Background Takayasu’s arteritis (TA) is a large-vessel vasculitis pathologically characterized by granulomatous necrotizing vasculitis with giant cells. Although the cause of TA is still unclear, genetic factors as well as immunological abnormalities, particularly the overactivation of Th1 and Th-17, are considered to play important roles in the pathogenesis of this disease. Eosinophilic gastroenteritis (EGE) is a type of refractory inflammation in which numerous eosinophils infiltrate the inflammatory area. It is known that the overactivation of Th2 is associated with the pathogenesis of EGE, although the cause of EGE is still unclear. The immunological abnormalities in TA are therefore thought to be different from those in EGE. To date, no cases of complication of TA and EGE have been reported. Case presentations An 18 year-old female was diagnosed with EGE and treated with prednisolone. At 6 months after completion of the treatment, the patient experienced chest pain, and was diagnosed with TA. TH1 and TH17 immunity are thought to be involved with TA, while TH2 are considered to be involved with EGE. In this case, the expression of IL-17 mRNA in the colon mucosa greatly decreased after prednisolone treatment for EGE. Conclusions This is the first report of TA complicated with EGE, and the overactivation of TH17 is considered to be associated with the pathogenesis of these two diseases.
- Published
- 2018
21. Syphilis infection throughout the whole gastrointestinal tract
- Author
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Shin Kashima, Mikihiro Fujiya, Satoshi Fujii, Masami Ijiri, Nobuhiro Ueno, Toshikatu Okumura, and Tomohisa Watari
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Adult ,Pathology ,medicine.medical_specialty ,MEDLINE ,Stomach Diseases ,Syphilis infection ,Penicillins ,Endoscopy, Gastrointestinal ,03 medical and health sciences ,Colonic Diseases ,0302 clinical medicine ,Medicine ,Humans ,030212 general & internal medicine ,Syphilis ,Gastrointestinal tract ,medicine.diagnostic_test ,business.industry ,Ileal Diseases ,Gastroenterology ,Dermatology ,Endoscopy ,Anti-Bacterial Agents ,030211 gastroenterology & hepatology ,Female ,business - Published
- 2016
22. Tu1966 PREDICTION OF RELAPSE IN PATIENTS WITH ULCERATIVE COLITIS USING CONVENTIONAL ENDOSCOPY AND AUTOFLUORESCENCE IMAGING
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Masami Ijiri, Keitaro Takahashi, Takuya Iwama, Katsuyoshi Ando, Mikihiro Fujiya, Shin Kashima, Yu Kobayashi, Hiroki Tanabe, Talahiro Sasaki, Kentaro Moriichi, Nobuhiro Ueno, Toshikatsu Okumura, Takehito Kunogi, and Yuuki Murakami
- Subjects
medicine.medical_specialty ,Autofluorescence ,medicine.diagnostic_test ,business.industry ,Gastroenterology ,medicine ,Radiology, Nuclear Medicine and imaging ,In patient ,Radiology ,business ,medicine.disease ,Ulcerative colitis ,Endoscopy - Published
- 2019
23. The Incidence and Risk Factors of Venous Thromboembolism in Japanese Inpatients With Inflammatory Bowel Disease: A Retrospective and Prospective Study
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Katsuyoshi, Ando, primary, Mikihiro, Fujiya, additional, Yoshiki, Nomura, additional, Nobuhiro, Ueno, additional, Yuhei, Inaba, additional, Yuya, Sugiyama, additional, Takuya, Iwama, additional, Masami, Ijiri, additional, Keitaro, Takahashi, additional, Kazuyuki, Tanaka, additional, Takuma, Goto, additional, Shin, Kashima, additional, Junpei, Sasajima, additional, Kentaro, Moriichi, additional, Yusuke, Mizukami, additional, Satoshi, Yamada, additional, Hiroshi, Nakase, additional, and Toshikatsu, Okumura, additional
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- 2018
- Full Text
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24. Efficacy of Quantitated Autofluorescence Imaging Endoscopy in Patients With Ulcerative Colitis: A Multicenter Study
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Kentaro, Moriichi, primary, Mikihiro, Fujiya, additional, Yuya, Sugiyama, additional, Takuya, Iwama, additional, Masami, Ijiri, additional, Kazuyuki, Tanaka, additional, Keitaro, Takahashi, additional, Aki, Sakatani, additional, Katsuyoshi, Ando, additional, Yoshiki, Nomura, additional, Nobuhiro, Ueno, additional, Shin, Kashima, additional, Yuhei, Inaba, additional, Takahiro, Ito, additional, and Toshikatsu, Okumura, additional
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- 2018
- Full Text
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25. Tu1532 - Neuroendocrine Carcinoma of the Gallbladder: Prognostic Factors and Optimal Treatment Options
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Takuya Iwama, Keitaro Takahashi, Katsuyoshi Ando, Takuma Goto, Masami Ijiri, Yoshiki Nomura, Shugo Fujibayashi, Hiroki Sato, Akihiro Hayashi, Yusuke Mizukami, Kentaro Moriichi, Shuhei Takauji, Shin Kashima, Hidemasa Kawabata, Junpei Sasajima, Mikihiro Fujiya, Tetsuhiro Okada, Toshikatsu Okumura, and Nobuhiro Ueno
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medicine.medical_specialty ,medicine.anatomical_structure ,Hepatology ,business.industry ,Optimal treatment ,Internal medicine ,Gallbladder ,Gastroenterology ,Medicine ,Neuroendocrine carcinoma ,business - Published
- 2018
26. The incidence and risk factors of venous thromboembolism in Japanese inpatients with inflammatory bowel disease: a retrospective cohort study
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Yuuya Sugiyama, Takuya Iwama, Kentaro Moriichi, Nobuhiro Ueno, Aki Sakatani, Katsuyoshi Ando, Yoshiki Nomura, Keitaro Takahashi, Kazuyuki Tanaka, Yuhei Inaba, Masami Ijiri, Mikihiro Fujiya, Toshikatsu Okumura, Shin Kashima, and Yusuke Mizukami
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medicine.medical_specialty ,lcsh:Medicine ,Inflammatory bowel disease ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,cardiovascular diseases ,Gastrointestinal cancer ,lcsh:RC799-869 ,Risk factor ,Univariate analysis ,business.industry ,Mortality rate ,Incidence (epidemiology) ,lcsh:R ,Gastroenterology ,Retrospective cohort study ,equipment and supplies ,medicine.disease ,Ulcerative colitis ,digestive system diseases ,Risk factors ,030220 oncology & carcinogenesis ,lcsh:Diseases of the digestive system. Gastroenterology ,Original Article ,030211 gastroenterology & hepatology ,business ,Venous thromboembolism - Abstract
Background/aims Venous thromboembolism (VTE) is a major extraintestinal manifestation in inflammatory bowel disease (IBD), regarded as an independent risk factor for VTE according to reports from Western countries. However, the incidence and risk factors of VTE in Asian IBD patients are not fully understood. We aimed to reveal the incidence and risk factors of VTE in Japanese IBD inpatients. Methods The incidence of VTE in inpatients with IBD (n=340), gastrointestinal cancers (n=557), and other gastrointestinal diseases (n=569) treated at our hospital from 2009 to 2013 was retrospectively investigated. The characteristics and laboratory data of IBD inpatients with and without VTE were compared in univariate and multivariate analyses. Clinical courses of VTE in IBD were surveyed. Results VTE was detected in 7.1% of IBD inpatients, significantly higher than in gastrointestinal cancer inpatients (2.5%) and inpatients with other gastrointestinal diseases (0.88%). The incidence of VTE in ulcerative colitis (UC) patients (16.7%) was much higher than that in those with Crohn's disease (3.6%). In the univariate analysis, the risk factors were an older age, central venous catheter, prednisolone, surgery, low serum albumin, high serum C-reactive protein and D-dimer. According to a multivariate analysis, >50 years of age and surgery were the only risk factors. The in-hospital mortality rate of IBD inpatients with VTE was 4.2%. Conclusions The incidence of VTE with IBD, especially UC, was found to be high compared with other digestive disease, which was almost equivalent to that of Western countries. The efficacy of prophylaxis needs to be investigated in Asian IBD patients.
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- 2018
27. Quantification of autofluorescence imaging can accurately and objectively assess the severity of ulcerative colitis
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Shin Kashima, Kentaro Moriichi, Junpei Sasajima, Mikihiro Fujiya, Masami Ijiri, Kazuyuki Tanaka, Shugo Fujibayashi, Takahiro Ito, Yutaka Kohgo, Yusuke Saitoh, Aki Sakatani, Yoshiki Nomura, Yuhei Inaba, Nobuhiro Ueno, Tatsuya Dokoshi, Hiroki Tanabe, Takuma Gotoh, and Katsuyoshi Ando
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Male ,medicine.medical_specialty ,Gastroenterology ,Severity of Illness Index ,Endoscopy, Gastrointestinal ,Cohen's kappa ,Internal medicine ,Severity of illness ,medicine ,Humans ,Prospective Studies ,Prospective cohort study ,Observer Variation ,Receiver operating characteristic ,business.industry ,Optical Imaging ,Middle Aged ,medicine.disease ,Ulcerative colitis ,Autofluorescence ,Colitis, Ulcerative ,Female ,Active inflammation ,Nuclear medicine ,business ,Kappa - Abstract
No endoscopic examination has been able to evaluate severity of ulcerative colitis (UC) by quantification. This prospective study investigated the efficacy of quantifying autofluorescence imaging (AFI) to assess the severity of UC, which captures the fluorescence emitted from intestinal tissue and then quantifies the intensity using an image-analytical software program. Eleven endoscopists separately evaluated 135 images of conventional endoscopy (CE) and AFI from a same lesion. A CE image corresponding to Mayo endoscopic subscore 0 or 1 was defined as being inactive. The fluorescence intensities of AFI were quantified using an image-analytical software program (F index; FI). Active inflammation was defined when Matts’ histological grade was 2 or more. A cut-off value of the FI for active inflammation was determined using a receiver operating characteristic (ROC) analysis. The inter-observer consistency was calculated by unweighted kappa statistics. The correlation coefficient for the FI was inversely related to the histological severity (r = −0.558, p
- Published
- 2015
28. Ferrichrome identified from Lactobacillus casei ATCC334 induces apoptosis through its iron-binding site in gastric cancer cells
- Author
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Mikihiro Fujiya, Hiroaki Konishi, Hiroki Tanaka, Katsuya Ikuta, Junpei Sasajima, Masami Ijiri, Shin Kashima, Nobuhiro Ueno, Kentaro Moriichi, and Toshikatsu Okumura
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0301 basic medicine ,ferrichrome ,Lactobacillus casei ,Siderophore ,Iron ,Apoptosis ,anticancer ,iron binding ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,Stomach Neoplasms ,Cell Line, Tumor ,Animals ,Humans ,Binding site ,Receptor ,anti-tumor ,c-Jun N-terminal kinase ,Ferrichrome ,DNA damage-inducible transcript 3 ,iron chelation ,Binding Sites ,biology ,siderophores ,General Medicine ,biology.organism_classification ,Xenograft Model Antitumor Assays ,Caspase 9 ,Molecular Docking Simulation ,Lacticaseibacillus casei ,030104 developmental biology ,Biochemistry ,chemistry ,probiotics ,Cell culture ,Cancer cell ,Gastric cancer ,Intracellular - Abstract
This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/), Ferrichrome is known to be a siderophore, but it was recently identified as a tumor-suppressive molecule derived from Lactobacillus casei ATCC334 ( L. casei). In the present study, we investigated the effects of ferrichrome in gastric cancer cells. Cell lines and xenograft models treated with ferrichrome demonstrated growth suppression. The expression levels of cleaved poly (adenosine diphosphate-ribose) polymerase, and cleaved caspase-9 were increased by ferrichrome treatment. Although the tumor-suppressive effects of ferrichrome were almost completely diminished by the iron chelation, the reduction in the intracellular iron by ferrichrome did not correlate with its tumor-suppressive effects. An exhaustive docking simulation indicated that iron-free ferrichrome can make stable conformations with various mammalian molecules, including transporters and receptors. In conclusion, probiotic-derived ferrichrome induced apoptosis in gastric cancer cells. The iron binding site of ferrichrome is the structure responsible for its tumor suppressive function.
- Published
- 2017
29. Ferrichrome, A Tumor Suppressive Molecule Derived from Lactobacillus Casei, Inhibits the Progression of Colorectal Cancer via the Endoplasmic Reticulum Stress Pathway
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Masami Ijiri, Hiroki Tanaka, Katsuya Ikuta, Shin Kashima, Keitaro Takahashi, Junpei Sasajima, Takuma Goto, Katsuyoshi Ando, Hiroaki Konishi, Toshikatsu Okumura, Kentaro Moriichi, Mikihiro Fujiya, Kazuyuki Tanaka, Shugo Fujibayashi, and Nobuhiro Ueno
- Subjects
chemistry.chemical_compound ,Lactobacillus casei ,Hepatology ,Biochemistry ,chemistry ,biology ,Colorectal cancer ,Endoplasmic reticulum ,Gastroenterology ,medicine ,biology.organism_classification ,medicine.disease ,Ferrichrome - Published
- 2017
30. Sa1816 Evaluation of Disease Activity in Patients With UC By White Light, Magnifying and Autofluorescence Imaging Endoscopy
- Author
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Junpei Sasajima, Yoshiki Nomura, Shin Kashima, Masami Ijiri, Mikihiro Fujiya, Takuya Iwama, Nobuhiro Ueno, Kazuyuki Tanaka, Kentaro Moriichi, Hiroki Sato, Tatsuya Utsumi, Shugo Fujibayashi, Aki Sakatani, and Takuma Goto
- Subjects
Disease activity ,medicine.medical_specialty ,Autofluorescence ,medicine.diagnostic_test ,business.industry ,Gastroenterology ,White light ,medicine ,Radiology, Nuclear Medicine and imaging ,In patient ,Radiology ,business ,Endoscopy - Published
- 2016
31. Ferrichrome identified from Lactobacillus casei ATCC334 induces apoptosis through its iron-binding site in gastric cancer cells.
- Author
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Masami Ijiri, Mikihiro Fujiya, Hiroaki Konishi, Hiroki Tanaka, Nobuhiro Ueno, Shin Kashima, Kentaro Moriichi, Junpei Sasajima, Katsuya Ikuta, and Toshikatsu Okumura
- Subjects
FERRICHROMES ,CYCLIC peptides ,SIDEROPHORES ,MICROBIAL metabolites ,LACTOBACILLUS casei - Abstract
Ferrichrome is known to be a siderophore, but it was recently identified as a tumor-suppressive molecule derived from Lactobacillus casei ATCC334 (L. casei). In the present study, we investigated the effects of ferrichrome in gastric cancer cells. Cell lines and xenograft models treated with ferrichrome demonstrated growth suppression. The expression levels of cleaved poly (adenosine diphosphate-ribose) polymerase, and cleaved caspase-9 were increased by ferrichrome treatment. Although the tumor-suppressive effects of ferrichrome were almost completely diminished by the iron chelation, the reduction in the intracellular iron by ferrichrome did not correlate with its tumor-suppressive effects. An exhaustive docking simulation indicated that iron-free ferrichrome can make stable conformations with various mammalian molecules, including transporters and receptors. In conclusion, probiotic-derived ferrichrome induced apoptosis in gastric cancer cells. The iron binding site of ferrichrome is the structure responsible for its tumor suppressive function. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
32. P-153 Autofluorescence Imaging Is Useful to Assess the Severity of Ulcerative Colitis
- Author
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Kei Sakatani, Mikihiro Fujiya, Tatsuya Dokoshi, Kentaro Moriichi, Shin Kashima, Takahiro Ito, Yutaka Kohgo, Katsuyoshi Ando, Nobuhiro Ueno, Kazuyuki Tanaka, Yuhei Inaba, and Masami Ijiri
- Subjects
Autofluorescence ,Pathology ,medicine.medical_specialty ,business.industry ,Gastroenterology ,Immunology and Allergy ,Medicine ,business ,medicine.disease ,Ulcerative colitis - Published
- 2013
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