662 results on '"Masaaki Kobayashi"'
Search Results
2. Endovascular treatment for infectious abdominal aortic aneurysm: A case series
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Makoto Matsuura, Satoshi Kuroyanagi, Onnichi Furuya, Masaaki Kobayashi, Shintarou Okuda, and Masatoshi Komooka
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Aneurysm ,EVAR ,Infected ,Mycotic ,Aorta ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 ,Surgery ,RD1-811 - Abstract
This retrospective study examined 12 patients with infectious abdominal aortic aneurysms who underwent endovascular aneurysm repair (EVAR). The majority were older males with saccular aneurysms. The 30-day and 1-year mortality rates were 33 % and 50 %, respectively. Two patients required reoperation for infection extension. Among the six patients with saccular aneurysms, complete aneurysm shrinkage was observed post-EVAR. The study suggests EVAR may effectively treat certain infectious abdominal aneurysms, particularly saccular types. However, more evidence is needed to determine its suitability. Careful patient selection, considering factors such as aneurysm morphology, is critical as infection control can be insufficient in some cases. Overall, this small case series indicates that EVAR may be a suitable option for select cases of saccular infectious abdominal aortic aneurysms.
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- 2024
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3. Routine hypercapnic challenge after cervical spinal hemisection affects the size of phrenic motoneurons
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Kenta Kawamura, Masaaki Kobayashi, and Kazuhide Tomita
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Medicine ,Science - Abstract
Abstract After an individual experiences a cervical cord injury, the cell body's adaptation to the smaller size of phrenic motoneurons occurs within several weeks. It is not known whether a routine hypercapnic load can alter this adaptation of phrenic motoneurons. We investigated this question by using rats with high cervical cord hemisection. The rats were divided into four groups: control, hypercapnia, sham, and sham hypercapnia. Within 72 h post-hemisection, the hypercapnia groups began a hypercapnic challenge (20 min/day, 4 times/week for 3 weeks) with 7% CO2 under awake conditions. After the 3-week challenge, the phrenic motoneurons in all of the rats were retrogradely labeled with horseradish peroxidase, and the motoneuron sizes in each group were compared. The average diameter, cross-sectional area, and somal surface area of stained phrenic motoneurons as analyzed by software were significantly smaller in only the control group compared to the other groups. The histogram distribution was unimodal, with larger between-group size differences for motoneurons in the horizontal plane than in the transverse plane. Our findings indicate that a routine hypercapnic challenge may increase the input to phrenic motoneurons and alter the propensity for motoneuron adaptations.
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- 2023
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4. Efficacy of ripasudil in reducing intraocular pressure and medication score for ocular hypertension with inflammation and corticosteroid
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Ryoji Yanai, Sho-Hei Uchi, Masaaki Kobayashi, Tomohiko Nagai, Shinichiro Teranishi, Makiko Wakuta, and Kazuhiro Kimura
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intraocular pressure ,medication score ,uveitis ,glaucoma ,rho kinase inhibitor ,Ophthalmology ,RE1-994 - Abstract
AIM: To investigate the efficacy of ripasudil, a Rho kinase inhibitor, in reducing intraocular pressure (IOP) and medication scores of anti-glaucoma drugs in patients with ocular hypertension with inflammation and corticosteroid. METHODS: The study included 11 patients diagnosed with ocular hypertension with inflammation and corticosteroid, all of whom were prescribed ripasudil eye drops and followed up for at least 2y after the initiation of treatment. IOP was measured using a non-contact tonometer before enrollment and at each follow-up visit. The medication score of glaucoma eye drops was calculated for each patient. RESULTS: The mean IOP (26.4±2.9 mm Hg before treatment) significantly decreased after ripasudil therapy (13.7±3.3 mm Hg at 3mo) and remained stable in the low-teens during the 2-year follow-up period (P
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- 2023
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5. Molecular Evolution of GII.P31/GII.4_Sydney_2012 Norovirus over a Decade in a Clinic in Japan
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Hiroshi Ushijima, Sheikh Ariful Hoque, Yuki Akari, Ngan Thi Kim Pham, Tung Phan, Shuichi Nishimura, Masaaki Kobayashi, Kumiko Sugita, Shoko Okitsu, Satoshi Komoto, Aksara Thongprachum, Pattara Khamrin, Niwat Maneekarn, and Satoshi Hayakawa
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norovirus ,GII.P31/GII.4_Sydney_2012 ,epidemics ,ORFs ,molecular evolution ,deduced amino acid sequences ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Norovirus (NoV) genogroup II, polymerase type P31, capsid genotype 4, Sydney_2012 variant (GII.P31/GII.4_Sydney_2012) has been circulating at high levels for over a decade, raising the question of whether this strain is undergoing molecular alterations without demonstrating a substantial phylogenetic difference. Here, we applied next-generation sequencing to learn more about the genetic diversity of 14 GII.P31/GII.4_Sydney_2012 strains that caused epidemics in a specific region of Japan, with 12 from Kyoto and 2 from Shizuoka, between 2012 and 2022, with an emphasis on amino acid (aa) differences in all three ORFs. We found numerous notable aa alterations in antigenic locations in the capsid region (ORF2) as well as in other ORFs. In all three ORFs, earlier strains (2013–2016) remained phylogenetically distinct from later strains (2019–2022). This research is expected to shed light on the evolutionary properties of dominating GII.P31/GII.4_Sydney_2012 strains, which could provide useful information for viral diarrhea prevention and treatment.
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- 2024
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6. Expression and localization of tight junction-related proteins in adult rat pituitary stem/progenitor cell niches
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Saishu YOSHIDA, Hideaki YURINO, Masaaki KOBAYASHI, Naoto NISHIMURA, Kentaro YANO, Ken FUJIWARA, Shin-ichi HASHIMOTO, Takako KATO, and Yukio KATO
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rat pituitary ,stem cell niche ,stem/progenitor cells ,tight junction ,Reproduction ,QH471-489 ,Internal medicine ,RC31-1245 - Abstract
Pituitary endocrine cells are supplied by Sox2-expressing stem/progenitor cells in the anterior lobe of the adult pituitary gland. These SOX2-positive cells are maintained in two types of microenvironments (niches): the marginal cell layer (MCL)-niche and the parenchymal-niche. Recently, we isolated dense SOX2-positive cell clusters from the parenchymal-niche by taking advantage of their resistance to protease treatment as parenchymal stem/progenitor cell (PS)-clusters. In the present study, by analyzing these isolated PS-clusters, we attempted to identify novel structural characteristics of pituitary stem/progenitor cell niches. Quantitative real-time PCR showed that tight junction-related genes were distinctly expressed in the isolated PS-clusters. Immunocytostaining showed that the tight junction molecules, ZO-1 and occludin, were localized in the apical membrane facing the pseudo-follicle-like structure of the isolated PS-clusters regardless of the expression of S100β, which distinguishes the sub-population of SOX2-positive cells. Furthermore, immunohistochemistry of the pituitary glands of adult rats clearly demonstrated that ZO-1 and occludin were densely present in the parenchymal-niche encircling the pseudo-follicle, while they were observed in the apical membrane in the MCL-niche facing the residual lumen. Collectively, these tight junction-related proteins might be involved in the architecture and maintenance of the plasticity of pituitary stem/progenitor cell niches.
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- 2022
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7. Intraoperative rupture of intracerebral aneurysm immediately after meningioma resection: a case report
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Shingo Tanaka, Masaaki Kobayashi, Toshiya Ichinose, Nozomu Oikawa, Masashi Kinoshita, Akifumi Yoshikawa, Naoyuki Uchiyama, and Mitsutoshi Nakada
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meningioma ,Brain tumor co-existing with cerebral aneurysm ,Feeder related aneurysm ,Intraoperative ruptured aneurysm ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Background Meningiomas and unruptured cerebral aneurysms (UCAs) rarely coexist. However, the treatment strategy remains to be fully elucidated. This report is a first report that UCA related to the tumor feeder intraoperatively ruptured when the meningioma was resected. Case presentation Herein, we present a case of meningioma coexisting with contralateral UCA related to a tumor feeder. Immediately after the meningioma was resected, intraoperative acute brain swelling due to rupture of the contralateral aneurysm appeared. The swollen brain protruding into the epidural space was resected, following contralateral ruptured aneurysm was performed by endovascular surgery. Intensive neurological treatment was administered and the patient gradually recovered. Conclusion This report highlights the possibility of intraoperative UCA rupture related to the tumor feeder when the meningioma is resected.
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- 2022
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8. 3D-printed eye model: Simulation of intraocular pressure
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Hidenaga Kobashi and Masaaki Kobayashi
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Medicine ,Science - Abstract
Purpose To develop artificial eye models using 3D printing and to evaluate the correlation between different corneal thicknesses and intraocular pressures (IOPs). Methods We designed 7 artificial eye models using a computer-aided design system and fabricated them using 3D printing. Corneal curvature and axial length were based on the Gullstrand eye model. Hydrogels were injected into the vitreous cavity, and seven different corneal thicknesses (200 to 800 μm) were prepared. In this proposed design, we also produced different corneal stiffnesses. A Tono-Pen AVIA tonometer was used by the same examiner to perform five consecutive IOP measurements in each eye model. Results Different eye models were ideally created using 3D printing. IOP measurements were successfully performed in each eye model. The corneal thickness was significantly correlated with IOP (R2 = 0.927; 𝑃Conclusion The 3D-printed eye model is useful for evaluating IOP measurements. This technique might be a promising alternative to the conventional porcine eye model.
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- 2023
9. Interferon signaling and hypercytokinemia-related gene expression in the blood of antidepressant non-responders
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Hirotaka Yamagata, Ryouichi Tsunedomi, Toshiharu Kamishikiryo, Ayumi Kobayashi, Tomoe Seki, Masaaki Kobayashi, Kosuke Hagiwara, Norihiro Yamada, Chong Chen, Shusaku Uchida, Hiroyuki Ogihara, Yoshihiko Hamamoto, Go Okada, Manabu Fuchikami, Jun-ichi Iga, Shusuke Numata, Makoto Kinoshita, Takahiro A. Kato, Ryota Hashimoto, Hiroaki Nagano, Shuichi Ueno, Yasumasa Okamoto, Tetsuro Ohmori, and Shin Nakagawa
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Antidepressant ,Biomarkers ,Gene expression ,Hypercytokinemia ,Interferon ,Peripheral blood ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Only 50% of patients with depression respond to the first antidepressant drug administered. Thus, biomarkers for prediction of antidepressant responses are needed, as predicting which patients will not respond to antidepressants can optimize selection of alternative therapies. We aimed to identify biomarkers that could predict antidepressant responsiveness using a novel data-driven approach based on statistical pattern recognition. We retrospectively divided patients with major depressive disorder into antidepressant responder and non-responder groups. Comprehensive gene expression analysis was performed using peripheral blood without narrowing the genes. We designed a classifier according to our own discrete Bayes decision rule that can handle categorical data. Nineteen genes showed differential expression in the antidepressant non-responder group (n = 15) compared to the antidepressant responder group (n = 15). In the training sample of 30 individuals, eight candidate genes had significantly altered expression according to quantitative real-time polymerase chain reaction. The expression of these genes was examined in an independent test sample of antidepressant responders (n = 22) and non-responders (n = 12). Using the discrete Bayes classifier with the HERC5, IFI6, and IFI44 genes identified in the training set yielded 85% discrimination accuracy for antidepressant responsiveness in the 34 test samples. Pathway analysis of the RNA sequencing data for antidepressant responsiveness identified that hypercytokinemia- and interferon-related genes were increased in non-responders. Disease and biofunction analysis identified changes in genes related to inflammatory and infectious diseases, including coronavirus disease. These results strongly suggest an association between antidepressant responsiveness and inflammation, which may be useful for future treatment strategies for depression.
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- 2023
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10. Benzalkonium chloride-induced myofibroblastic transdifferentiation of Tenon’s capsule fibroblasts is inhibited by coculture with corneal epithelial cells or by interleukin-10
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Chiemi Yamashiro, Kazuhiro Tokuda, Yuka Kobayashi, Fumiaki Higashijima, Takuya Yoshimoto, Manami Ota, Tadahiko Ogata, Atsushige Ashimori, Masaaki Kobayashi, Makoto Hatano, Sho-Hei Uchi, Makiko Wakuta, Shinichiro Teranishi, and Kazuhiro Kimura
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Medicine ,Science - Abstract
Abstract Benzalkonium chloride (BAC) is used as a preservative in eyedrops but induces subconjunctival fibrosis that can result in failure of glaucoma surgery. Tenon’s capsule fibroblasts in subconjunctival tissue interact with the corneal epithelium through tear fluid. With the use of a coculture system, we have now investigated the effect of human corneal epithelial (HCE) cells on myofibroblastic transdifferentiation of human Tenon fibroblasts (HTFs) induced by BAC (5 × 10−6%). Immunofluorescence and immunoblot analyses revealed that the BAC-induced expression of α smooth muscle actin (αSMA) in HTFs was suppressed by coculture of these cells with HCE cells (p
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- 2021
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11. Optimized protocol for the extraction of RNA and DNA from frozen whole blood sample stored in a single EDTA tube
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Hirotaka Yamagata, Ayumi Kobayashi, Ryouichi Tsunedomi, Tomoe Seki, Masaaki Kobayashi, Kosuke Hagiwara, Chong Chen, Shusaku Uchida, Go Okada, Manabu Fuchikami, Toshiharu Kamishikiryo, Jun-ichi Iga, Shusuke Numata, Makoto Kinoshita, Takahiro A. Kato, Ryota Hashimoto, Hiroaki Nagano, Yasumasa Okamoto, Shuichi Ueno, Tetsuro Ohmori, and Shin Nakagawa
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Medicine ,Science - Abstract
Abstract Cryopreservation of whole blood is useful for DNA collection, and clinical and basic research. Blood samples in ethylenediaminetetraacetic acid disodium salt (EDTA) tubes stored at − 80 °C are suitable for DNA extraction, but not for high-quality RNA extraction. Herein, a new methodology for high-quality RNA extraction from human blood samples is described. Quickly thawing frozen whole blood on aluminum blocks at room temperature could minimize RNA degradation, and improve RNA yield and quality compared with thawing the samples in a 37 °C water bath. Furthermore, the use of the NucleoSpin RNA kit increased RNA yield by fivefold compared with the PAXgene Blood RNA Kit. Thawing blood samples on aluminum blocks significantly increased the DNA yield by ~ 20% compared with thawing in a 37 °C water bath or on ice. Moreover, by thawing on aluminum blocks and using the NucleoSpin RNA and QIAamp DNA Blood kits, the extraction of RNA and DNA of sufficient quality and quantity was achieved from frozen EDTA whole blood samples that were stored for up to 8.5 years. Thus, extracting RNA from frozen whole blood in EDTA tubes after long-term storage is feasible. These findings may help advance gene expression analysis, as well as biomarker research for various diseases.
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- 2021
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12. Inhibition of epithelial–mesenchymal transition in retinal pigment epithelial cells by a retinoic acid receptor-α agonist
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Yuka Kobayashi, Kazuhiro Tokuda, Chiemi Yamashiro, Fumiaki Higashijima, Takuya Yoshimoto, Manami Ota, Tadahiko Ogata, Atsushige Ashimori, Makoto Hatano, Masaaki Kobayashi, Sho-Hei Uchi, Makiko Wakuta, and Kazuhiro Kimura
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Medicine ,Science - Abstract
Abstract Epithelial–mesenchymal transition (EMT) in retinal pigment epithelial (RPE) cells plays a key role in proliferative retinal diseases such as age-related macular degeneration by contributing to subretinal fibrosis. To investigate the potential role of retinoic acid receptor-α (RAR-α) signaling in this process, we have now examined the effects of the RAR-α agonist Am580 on EMT induced by transforming growth factor-β2 (TGF-β2) in primary mouse RPE cells cultured in a three-dimensional type I collagen gel as well as on subretinal fibrosis in a mouse model. We found that Am580 inhibited TGF-β2-induced collagen gel contraction mediated by RPE cells. It also attenuated the TGF-β2-induced expression of the mesenchymal markers α-smooth muscle actin, fibronectin, and collagen type I; production of pro-matrix metalloproteinase 2 and interleukin-6; expression of the focal adhesion protein paxillin; and phosphorylation of SMAD2 in the cultured RPE cells. Finally, immunofluorescence analysis showed that Am580 suppressed both the TGF-β2-induced translocation of myocardin-related transcription factor-A (MRTF-A) from the cytoplasm to the nucleus of cultured RPE cells as well as subretinal fibrosis triggered by laser-induced photocoagulation in a mouse model. Our observations thus suggest that RAR-α signaling inhibits EMT in RPE cells and might attenuate the development of fibrosis associated with proliferative retinal diseases.
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- 2021
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13. Distinct epigenetic signatures between adult-onset and late-onset depression
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Hirotaka Yamagata, Hiroyuki Ogihara, Koji Matsuo, Shusaku Uchida, Ayumi Kobayashi, Tomoe Seki, Masaaki Kobayashi, Kenichiro Harada, Chong Chen, Shigeo Miyata, Masato Fukuda, Masahiko Mikuni, Yoshihiko Hamamoto, Yoshifumi Watanabe, and Shin Nakagawa
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Medicine ,Science - Abstract
Abstract The heterogeneity of major depressive disorder (MDD) is attributed to the fact that diagnostic criteria (e.g., DSM-5) are only based on clinical symptoms. The discovery of blood biomarkers has the potential to change the diagnosis of MDD. The purpose of this study was to identify blood biomarkers of DNA methylation by strategically subtyping patients with MDD by onset age. We analyzed genome-wide DNA methylation of patients with adult-onset depression (AOD; age ≥ 50 years, age at depression onset
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- 2021
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14. Endoscopic Submucosal Dissection for Gastric Tube Carcinoma after Esophagectomy Contributes to Long-Term Outcomes
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Satoru Hashimoto, Hiroki Sato, Ken-ichi Mizuno, Kazuya Takahashi, Masafumi Takatsuna, Junji Yokoyama, Hiroshi Ichikawa, Manabu Takeuchi, Masaaki Kobayashi, and Shuji Terai
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Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
The incidence of gastric tube carcinoma (GTC) after esophagectomy for esophageal carcinoma has increased in recent years. Surgical removal of the reconstructed gastric tube is associated with high mortality, and endoscopic submucosal dissection (ESD) is a promising alternative. There are limited reports of ESD for GTC. This study investigated the efficacy and safety of ESD in GTC. This single-center retrospective study examined patients who underwent ESD for GTC after esophagectomy at our institution between 2003 and 2018. The curability of GTC with ESD was evaluated histologically according to the Japanese Gastric Cancer Treatment Guidelines. Patient characteristics and procedural and long-term outcomes were analyzed. Overall, 31 patients (29 men and 2 women; median age, 73 years) with 45 GTC lesions underwent ESD. The mean period between primary esophagectomy and the diagnosis of GTC was 10.6 years. Bleeding during ESD was noted in two patients (6.5%). No other adverse or fatal events such as perforation were noted. Complete resection and curative resection were documented in 80.6% and 48.4% of cases, respectively. The 3-year and 5-year overall survival rates were 67.6% and 47.7%, respectively. The 3-year and 5-year disease-specific survival rates were 100% and 92.9%, respectively. One patient died of GTC, and fourteen patients died of other diseases, including primary carcinoma in five cases. ESD was safe and provided good long-term outcomes in patients with GTC. Regular long-term gastroscopy is required for the early detection of GTC. Patients with GTC after esophagectomy for esophageal carcinoma have a high risk of other primary carcinomas or comorbidities after ESD.
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- 2022
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15. Complete chloroplast genome sequence and phylogenetic analysis of wasabi (Eutrema japonicum) and its relatives
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Natsuko Haga, Masaaki Kobayashi, Nana Michiki, Tomoyuki Takano, Fujio Baba, Keiko Kobayashi, Hajime Ohyanagi, Jun Ohgane, Kentaro Yano, and Kyoko Yamane
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Medicine ,Science - Abstract
Abstract In Japan, two Eutrema species, wasabi (Eutrema japonicum, the important traditional Japanese condiment) and yuriwasabi (E. tenue), have been recognized as endemic species. We sequenced complete chloroplast (cp) genomes of seven wasabi and yuriwasabi accessions from Japan to study their phylogeny and evolution, using molecular dating of species divergence. Phylogenetic analyses of the complete cp DNA of these two Japanese species and five other Eurasian Eutrema species revealed that wasabi and yuriwasabi did not form a monophyletic group. One yuriwasabi accession (Gifu) formed a clade with E. yunnanense from China, indicating that this accession should be considered as a different species from the other yuriwasabi accessions. We reveal that Japanese Eutrema species diverged from the ‘E. yunnanense–yuriwasabi (Gifu)’ clade approximately 1.3 million years ago (Mya), suggesting that the connection between Japan and the Eurasian continent has existed more recently than the Quaternary period. The abundance of cp sequence data in this study also allowed the detection of genetic differentiation among wasabi cultivars. The two polymorphic sites detected between ‘Fujidaruma’ and ‘Shimane No.3’ were used to develop genotyping markers. The cp genome information provided here will thus inform the evolutionary histories of Japanese Eutrema species and help in genotyping wasabi cultivars.
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- 2019
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16. GPR34 in spinal microglia exacerbates neuropathic pain in mice
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Akira Sayo, Hiroyuki Konishi, Masaaki Kobayashi, Kuniyuki Kano, Hiroki Kobayashi, Hideharu Hibi, Junken Aoki, and Hiroshi Kiyama
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Allodynia ,GPCR ,GPR34 ,LysoPS ,Microglia ,Neuroinflammation ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Background Neuropathic pain is caused by sensory nerve injury, but effective treatments are currently lacking. Microglia are activated in the spinal dorsal horn after sensory nerve injury and contribute to neuropathic pain. Accordingly, molecules expressed by these cells are considered potential targets for therapeutic strategies. Our previous gene screening study using a mouse model of motor nerve injury showed that the G-protein-coupled receptor 34 gene (GPR34) is induced by nerve injury. Because GPR34 is now considered a microglia-enriched gene, we explored the possibility that it might be involved in microglial activation in the dorsal horn in a mouse model of neuropathic pain. Methods mRNA expression of GPR34 and pro-inflammatory molecules was determined by quantitative real-time PCR in wild-type and GPR34-deficient mice with L4 spinal nerve injury. In situ hybridization was used to identify GPR34 expression in microglia, and immunohistochemistry with the microglial marker Iba1 was performed to examine microglial numbers and morphology. Mechanical sensitivity was evaluated by the von Frey hair test. Liquid chromatography–tandem mass spectrometry quantified expression of the ligand for GPR34, lysophosphatidylserine (LysoPS), in the dorsal horn, and a GPR34 antagonist was intrathecally administrated to examine the effect of inhibiting LysoPS-GPR34 signaling on mechanical sensitivity. Results GPR34 was predominantly expressed by microglia in the dorsal horn after L4 nerve injury. There were no histological differences in microglial numbers or morphology between WT and GPR34-deficient mice. However, nerve injury-induced pro-inflammatory cytokine expression levels in microglia and pain behaviors were significantly attenuated in GPR34-deficient mice. Furthermore, the intrathecal administration of the GPR34 antagonist reduced neuropathic pain. Conclusions Inhibition of GPR34-mediated signal by GPR34 gene deletion reduced nerve injury-induced neuropathic pain by suppressing pro-inflammatory responses of microglia without affecting their morphology. Therefore, the suppression of GPR34 activity may have therapeutic potential for alleviating neuropathic pain.
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- 2019
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17. Malignant lymphoma mimics miliary tuberculosis by diffuse micronodular radiographic findings
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Kyoko Yagyu, Masaaki Kobayashi, Takahiro Ueda, Riki Uenishi, Yuko Nakatsuji, and Haruhiko Matsushita
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Diagnostic imaging ,Follicular lymphoma ,Pulmonary secondary lymphoma ,Miliary opacity ,Diseases of the respiratory system ,RC705-779 - Abstract
This case reports rare findings on computed tomography of a manifestation of malignant lymphoma, in which diffuse lung shadows appeared as miliary nodules distributed throughout the lungs bilaterally. The patient had a history of surgical treatment of rectal cancer and had received chemotherapy for suspicious liver metastasis. At her current presentation for evaluation suspected miliary tuberculosis on chest radiography, subsequent liver biopsy revealed a mass infiltration of atypical lymphocytes, which was diagnosed as follicular lymphoma. The miliary tuberculosis was suspected more than neoplastic lesions, such as metastatic rectal cancer or malignant lymphoma. Despite repeated bacteriologic tests of various samples, including sputum, urine, bronchial secretion, peripheral blood, bone marrow aspiration, and gastric lavage, all results were negative for mycobacterium tuberculosis. Finally, multiple, small, mass lesions of lymphocytes were demonstrated in the lung obtained from video-assisted thoracic surgery, and a diagnosis of follicular lymphoma was given. The final interpretations of liver mass and military lung lesions were tumor involvement by the follicular lymphoma. This radiologic findings of multiple miliary opacities throughout the whole lungs confused definite diagnosis because these images were remarkably similar with miliary tuberculous. This case reminds us to consider a wide variety of differential diagnoses even we assumed to be familiar with radiographic imaging at first glance.
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- 2020
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18. Correction: Dendritic cells mediate the anti-inflammatory action of omega-3 long-chain polyunsaturated fatty acids in experimental autoimmune uveitis.
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Sho-Hei Uchi, Ryoji Yanai, Masaaki Kobayashi, Makoto Hatano, Yuka Kobayashi, Chiemi Yamashiro, Tomohiko Nagai, Kazuhiro Tokuda, Kip M Connor, Koh-Hei Sonoda, and Kazuhiro Kimura
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Medicine ,Science - Abstract
[This corrects the article DOI: 10.1371/journal.pone.0219405.].
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- 2020
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19. Influence of extended depth of focus intraocular lenses on visual field sensitivity.
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Makiko Takahashi, Chiemi Yamashiro, Takuya Yoshimoto, Yuka Kobayashi, Fumiaki Higashijima, Masaaki Kobayashi, Makoto Hatano, Manami Ohta, Tomohiko Nagai, Shinichiro Teranishi, Katsuyoshi Suzuki, Ryu Takabatake, and Kazuhiro Kimura
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Medicine ,Science - Abstract
PurposeTo investigate the influence of EDOF IOLs, TECNIS Symfony® (Johnson & Johnson Surgical Vision, Inc.), on visual field sensitivity and to compare the IOLs with other kinds of IOLs.MethodsThe subjects included the normal fellow eyes of patients who underwent the Humphrey Field Analyzer (HFA) 30-2 with Swedish Interactive Threshold Algorithm Fast within 6 months after cataract due to glaucoma or suspected glaucoma. Each parameter of HFA was compared among eyes implanted with TENIS Symfony® (EDOF group), diffractive bifocal IOLs (bifocal group), and monofocal IOLs (monofocal group).ResultsThe total of 76 eyes, including 24 eyes in the EDOF group, 26 eyes in the bifocal group, and 26 eyes in the monofocal group, were included in this study. Mean deviation (MD) of HFA was -0.24±0.58 dB in the EDOF group, -1.38±0.58 dB in the bifocal group, and 0.02±0.44 dB in the monofocal group. Foveal threshold (FT) of HFA was 35.8±1.6 dB in the EDOF group, 33.6±1.7 dB in the bifocal group, and 36.6±1.4 dB in the monofocal group. In both MD and FT, there was significant difference between the bifocal group and the others (pConclusionTECNIS Symfony® may have little influence on visual field sensitivity, whereas diffractive bifocal IOLs decrease visual field sensitivity.
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- 2020
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20. Repeated inversions within a pannier intron drive diversification of intraspecific colour patterns of ladybird beetles
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Toshiya Ando, Takeshi Matsuda, Kumiko Goto, Kimiko Hara, Akinori Ito, Junya Hirata, Joichiro Yatomi, Rei Kajitani, Miki Okuno, Katsushi Yamaguchi, Masaaki Kobayashi, Tomoyuki Takano, Yohei Minakuchi, Masahide Seki, Yutaka Suzuki, Kentaro Yano, Takehiko Itoh, Shuji Shigenobu, Atsushi Toyoda, and Teruyuki Niimi
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Science - Abstract
The harlequin ladybird beetle, Harmonia axyridis, has remarkable phenotypic diversity, with over 200 colour patterns. Here, Ando et al. show that this patterning is regulated by the transcription factor gene pannier and has diversified by repeated inversions and cis-regulatory modifications of pannier.
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- 2018
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21. Treatment of aneurysmal bone cysts using endoscopic curettage
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Hisaki Aiba, Masaaki Kobayashi, Yuko Waguri-Nagaya, Hideyuki Goto, Jun Mizutani, Satoshi Yamada, Hideki Okamoto, Masahiro Nozaki, Hiroto Mitsui, Shinji Miwa, Makoto Kobayashi, Kojiro Endo, Shiro Saito, Taeko Goto, and Takanobu Otsuka
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Endoscopy ,Endoscopic curettage ,Bone tumour ,Aneurysmal bone cyst ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Abstract Background Although aneurysmal bone cysts (ABCs) are benign tumours, they have the potential to be locally aggressive. Various treatment approaches, such as en bloc resection, open curettage, radiotherapy, sclerotherapy, and embolization have been proposed, but the most appropriate treatment should be selected after considering the risk of tumour recurrence and treatment complications. Endoscopic curettage (ESC) may be a less invasive alternative to open curettage for ABC treatment. We aimed to describe the use of ESC for the treatment of ABCs and to report our clinical outcomes, including the incidence rate of recurrence, radiological appearance at final follow-up, time to solid union, complications, and postoperative function. Methods Between 1998 and 2015, 30 patients (18 men and 12 women; mean age, 17.4 years) underwent ESC for the treatment of primary ABCs at our hospital (mean postoperative follow-up, 55 months). ESC was performed under arthroscopic guidance for direct visualization, and curettage extended until normal bone was observed in the medullary cavity. To investigate bone healing after ESC, we evaluated the consolidation of cysts at the final evaluation (based on the modified Neer classification) and time to solid union after surgery, which was defined as sufficient cortical bone thickness to prevent fracture and allow physical activities. Results Recurrence was identified in 3 cases (10%). Curative outcomes were obtained after repeated ESC or open curettage. A log-rank analysis indicated that age
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- 2018
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22. Treatment of simple bone cysts using endoscopic curettage: a case series analysis
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Hisaki Aiba, Masaaki Kobayashi, Yuko Waguri-Nagaya, Hideyuki Goto, Jun Mizutani, Satoshi Yamada, Hideki Okamoto, Masahiro Nozaki, Hiroto Mitsui, Shinji Miwa, Makoto Kobayashi, Kojiro Endo, Shiro Saito, Taeko Goto, and Takanobu Otsuka
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Endoscopy ,Endoscopic curettage ,Simple bone cysts ,Recurrence ,Minimally invasive procedure ,Orthopedic surgery ,RD701-811 ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Abstract Background Endoscopic curettage is considered applicable for the treatment of simple bone cysts with the expectation that it might be less invasive than open curettage. In this study, we investigated the efficacy of endoscopic curettage for the treatment of simple bone cysts. The goal was to investigate the incidence of cyst recurrence and bone healing after endoscopic curettage. Moreover, complications and functionality at the final follow-up were evaluated. Methods From 2003 to 2014, 37 patients with simple bone cysts underwent endoscopic curettage. Twenty-four were male and 13 were female, with a mean age of 14.7 years. Endoscopic curettage was performed with the support of an arthroscope via 7–8 mm holes penetrated by cannulated drills with a small incision. The cysts underwent curettage using angled curettes, rongeurs, and an electrical shaver until the normal bone was observed in the medullary cavity. To investigate the bone healing after endoscopic curettage, we evaluated the consolidation of the cyst at the final evaluation (Modified Neer Classification) and the time to solid union after operation, which was defined as the sufficient thickness of the cortical bone to prevent fracture and allow physical activities. Results Recurrence occurred in seven patients (18.9%). A log-rank analysis revealed that contact with the physis was associated with recurrence (p = 0.006). Among 31 patients (83.7%), the consolidation of cyst was considered healed at the final X-ray follow-up period, and in these patients, the mean time taken for solid union of cortical bone thinning was 4.0 months (standard deviation, 2.4). With regard to major complications of endoscopic curettage, a transient radial nerve palsy and two postoperative fractures occurred. The former problem was managed conservatively and the latter problems by transient internal fixation; these problems were managed without any further complications. All patients had a good postoperative function. Conclusions Endoscopic curettage might be a useful alternative as it is a minimally invasive procedure for the treatment of simple bone cysts. Considering the relatively smaller size of this study, further investigation should be necessary for deducing the reliable conclusion.
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- 2018
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23. Evaluating the effect of injecting triamcinolone acetonide in two sessions for preventing esophageal stricture after endoscopic submucosal dissection
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Satoru Hashimoto, Ken-ichi Mizuno, Kazuya Takahashi, Hiroki Sato, Junji Yokoyama, Manabu Takeuchi, Yuichi Sato, Masaaki Kobayashi, and Shuji Terai
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Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Background and study aims Several previous reports indicate that endoscopic injection of triamcinolone acetonide (TA) after widespread endoscopic submucosal dissection (ESD) is effective for preventing esophageal stricture. We investigated the efficacy of injecting TA in two sessions for preventing stricture formation post-ESD. Patients and methods Sixty-six consecutive patients with widespread mucosal defects that affected more than three-fourths of the circumference of the esophagus were included. The study group (n = 40) received TA injections over two sessions: immediately after and 14 days after ESD. The control group (n = 26) did not receive a TA injection. This study was performed retrospectively against historical controls. The primary endpoint of this study was frequency of stricture after TA injection. The secondary endpoint was number of required endoscopic balloon dilations (EBDs) after TA injection. Results The post-ESD stricture rate among patients who had subcircumferential mucosal defects was 45.7 % in the study group (16/35 patients), which was significantly lower than the rate of 73.9 % in the control group (17/23 patients; P = 0.031). The number of EBD procedures required was significantly lower in the study group (median 0, range 0 – 7) than in the control group (median 4, range 0 – 20; P
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- 2019
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24. Inhibitory effect of nintedanib on VEGF secretion in retinal pigment epithelial cells induced by exposure to a necrotic cell lysate.
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Makoto Hatano, Kazuhiro Tokuda, Yuka Kobayashi, Chiemi Yamashiro, Sho-Hei Uchi, Masaaki Kobayashi, and Kazuhiro Kimura
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Medicine ,Science - Abstract
Necrosis is a form of cell death that results in rupture of the plasma membrane and the release of cellular contents, and it can give rise to sterile inflammation in the retina and other tissues. The secretion of vascular endothelial growth factor (VEGF) by retinal pigment epithelial (RPE) cells contributes to retinal homeostasis as well as to pathological angiogenesis. We have now examined the effect of a necrotic cell lysate prepared from human RPE cells (NLR) on the release of VEGF by healthy RPE cells. We found that NLR markedly increased the release of VEGF from RPE cells and that this effect was attenuated by nintedanib, a multiple receptor tyrosine kinase inhibitor, whereas it was unaffected by inhibitors of NF-κB signaling or of caspase-1. NLR also induced the phosphorylation of extracellular signal-regulated kinase (Erk) and signal transducer and activator of transcription 3 (Stat3) in a manner sensitive to inhibition by nintedanib, although inhibitors of Erk and Stat3 signaling pathways did not affect NLR-induced VEGF secretion. In addition, nintedanib attenuated the development of choroidal neovascularization in mice. Our results have thus shown that a necrotic lysate of RPE cells induced VEGF secretion from healthy RPE cells and that this effect was mediated by receptor tyrosine kinase signaling. They therefore suggest that VEGF secretion by healthy RPE cells is a potential therapeutic target for retinal diseases associated with sterile inflammation and pathological angiogenesis.
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- 2019
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25. Dendritic cells mediate the anti-inflammatory action of omega-3 long-chain polyunsaturated fatty acids in experimental autoimmune uveitis.
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Sho-Hei Uchi, Ryoji Yanai, Masaaki Kobayashi, Makoto Hatano, Yuka Kobayashi, Chiemi Yamashiro, Tomohiko Nagai, Kazuo Tokuda, Kip M Connor, Koh-Hei Sonoda, and Kazuhiro Kimura
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Medicine ,Science - Abstract
We previously showed that dietary omega (ω)-3 long-chain polyunsaturated fatty acids (LCPUFAs) suppress inflammation in mice with experimental autoimmune uveitis (EAU). We have now investigated the role of antigen presenting cells (APCs) in this action of ω-3 LCPUFAs. C57BL/6 mice were fed a diet supplemented with ω-3 or ω-6 LCPUFAs for 2 weeks, after which splenocytes were isolated from the mice and cocultured with CD4+ T cells isolated from mice with EAU induced by injection of a human interphotoreceptor retinoid-binding protein peptide together with complete Freund's adjuvant. The proliferation of and production of interferon-γ and interleukin-17 by T cells from EAU mice in vitro were attenuated in the presence of splenocytes from ω-3 LCPUFA-fed mice as compared with those from mice fed ω-6 LCPUFAs. Splenocyte fractionation by magnetic-activated cell sorting revealed that, among APCs, dendritic cells (DCs) were the target of ω-3 LCPUFAs. Adoptive transfer of DCs from mice fed ω-3 LCPUFAs attenuated disease progression in EAU mice as well as the production of pro-inflammatory cytokines by T cells isolated from these latter animals. The proliferation of T cells from control Balb/c mice was also attenuated in the presence of DCs from ω-3 LCPUFA-fed mice as compared with those from ω-6 LCPUFA-fed mice. Furthermore, T cell proliferation in such a mixed lymphocyte reaction was inhibited by prior exposure of DCs from mice fed an ω-6 LCPUFA diet to ω-3 LCPUFAs in vitro. Our results thus suggest that DCs mediate the anti-inflammatory action of dietary ω-3 LCPUFAs in EAU.
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- 2019
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26. Comparison of shape quantification methods for genomic prediction, and genome-wide association study of sorghum seed morphology.
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Lisa Sakamoto, Hiromi Kajiya-Kanegae, Koji Noshita, Hideki Takanashi, Masaaki Kobayashi, Toru Kudo, Kentaro Yano, Tsuyoshi Tokunaga, Nobuhiro Tsutsumi, and Hiroyoshi Iwata
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Medicine ,Science - Abstract
Seed shape is an important agronomic trait with continuous variation among genotypes. Therefore, the quantitative evaluation of this variation is highly important. Among geometric morphometrics methods, elliptic Fourier analysis and semi-landmark analysis are often used for the quantification of biological shape variations. Elliptic Fourier analysis is an approximation method to treat contours as a waveform. Semi-landmark analysis is a method of superimposed points in which the differences of multiple contour positions are minimized. However, no detailed comparison of these methods has been undertaken. Moreover, these shape descriptors vary when the scale and direction of the contour and the starting point of the contour trace change. Thus, these methods should be compared with respect to the standardization of the scale and direction of the contour and the starting point of the contour trace. In the present study, we evaluated seed shape variations in a sorghum (Sorghum bicolor Moench) germplasm collection to analyze the association between shape variations and genome-wide single-nucleotide polymorphisms by genomic prediction (GP) and genome-wide association studies (GWAS). In our analysis, we used all possible combinations of three shape description methods and eight standardization procedures for the scale and direction of the contour as well as the starting point of the contour trace; these combinations were compared in terms of GP accuracy and the GWAS results. We compared the shape description methods (elliptic Fourier descriptors and the coordinates of superposed pseudo-landmark points) and found that principal component analysis of their quantitative descriptors yielded similar results. Different scaling and direction standardization procedures caused differences in the principal component scores, average shape, and the results of GP and GWAS.
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- 2019
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27. Enterovirus-Associated Hand-Foot and Mouth Disease and Neurological Complications in Japan and the Rest of the World
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Gabriel Gonzalez, Michael J. Carr, Masaaki Kobayashi, Nozomu Hanaoka, and Tsuguto Fujimoto
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hand-foot and mouth disease ,central nervous system complications ,encephalitis ,molecular characterization ,enterovirus a71 ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Enteroviruses (EVs) are responsible for extremely large-scale, periodic epidemics in pediatric cohorts, particularly in East and Southeast Asia. Clinical presentation includes a diverse disease spectrum, including hand-foot and mouth disease (HFMD), aseptic meningitis, encephalitis, acute flaccid paralysis, and acute flaccid myelitis. HFMD is predominantly attributable to EV-A types, including the major pathogen EV-A71, and coxsackieviruses, particularly CV-A6, CV-A16, and CV-A10. There have been multiple EV-A71 outbreaks associated with a profound burden of neurological disease and fatal outcomes in Asia since the early 1980s. Efficacious vaccines against EV-A71 have been developed in China but widespread pediatric vaccination programs have not been introduced in other countries. Encephalitis, as a consequence of complications arising from HFMD infection, leads to damage to the thalamus and medulla oblongata. Studies in Vietnam suggest that myoclonus is a significant indicator of central nervous system (CNS) complications in EV-A71-associated HFMD cases. Rapid response in HFMD cases in children is imperative to prevent the progression to a CNS infection; however, prophylactic and therapeutic agents have not been well established internationally, therefore surveillance and functional studies including development of antivirals and multivalent vaccines is critically important to reduce disease burden in pediatric populations.
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- 2019
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28. Differentiation capacities of PS-clusters, adult pituitary stem/progenitor cell clusters located in the parenchymal-niche, of the rat anterior lobe.
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Saishu Yoshida, Naoto Nishimura, Hideaki Yurino, Masaaki Kobayashi, Kotaro Horiguchi, Kentaro Yano, Shin-Ichi Hashimoto, Takako Kato, and Yukio Kato
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Medicine ,Science - Abstract
Pituitary endocrine cells are supplied by Sox2-expressing stem/progenitor cells in the anterior lobe of the adult pituitary. In relation to their microenvironment ("niche"), SOX2-positive cells exist in two types of niches; the marginal cell layer-niche and the parenchymal-niche. Recently, we isolated dense stem/progenitor cell clusters from the parenchymal-niche as parenchymal stem/progenitor cell (PS)-clusters. We classified these PS-clusters into three subtypes based on differences in S100β-expression (S100β-positive, -negative, and -mixed type), and reported that S100β-positive PS-clusters exhibited the capacity for differentiation into endocrine cells under 3-dimensional cultivation system. In the present study, we further characterized S100β-positive PS-clusters using an in vitro 2-dimensional cultivation system. The results demonstrated that S100β-positive PS-clusters in the 2-dimensional cultivation system proliferated more actively than S100β-negative clusters. Moreover, in 2-dimensional cultivation conditions, S100β-positive PS-clusters showed differentiation capacity into non-endocrine cells (Myogenin-, αSMA-, NG2-, or SOX17-positive cells) but not into endocrine cells, whereas S100β-negative PS-clusters did not. Collectively, PS-clusters were heterogeneous, exhibiting different proliferation and differentiation properties based on the difference in S100β-expression. Specifically, a part of SOX2-positive cells in the parenchymal-niche had capacities for differentiation into non-endocrine cells, and S100β-positive PS-clusters may be in more progressive stages toward differentiation than S100β-negative clusters.
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- 2018
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29. Magnifying image-enhanced endoscopy for collagenous colitis
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Masaaki Kobayashi, Takahiro Hoshi, Shin-ich Morita, Tsutomu Kanefuji, Takeshi Suda, Go Hasegawa, and Shuji Terai
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Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Background and study aims We report a case series of 5 patients with collagenous colitis (CC) presenting with chronic watery diarrhea, who were subjected to magnifying, chromoendoscopy and narrow band imaging (NBI). Magnifying chromoendoscopy revealed whitish-clouded, honeycomb-like appearance at orifices of the crypts. NBI with high magnification revealed irregular caliber variation of the subepithelial capillary network. Presence of these features corresponded with histological characteristics of CC indicated by thickening of the subepithelial collagen layer. We concluded that magnifying image-enhanced endoscopy is a reliable tool to diagnose CC.
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- 2017
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30. Utility of Over-the-Scope Clipping for Closure of a Persistent Post-Percutaneous Endoscopic Gastrostomy Fistula under Long-Term Steroid Therapy
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Yoshihisa Arao, Yuichi Sato, Satoru Hashimoto, Hiroki Honda, Kazumi Yoko, Masaaki Takamura, Ken-ichi Mizuno, and Masaaki Kobayashi
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Over-the-scope clipping ,Fistula ,Percutaneous endoscopic gastrostomy tube ,Fibrosis ,Steroids ,Internal medicine ,RC31-1245 ,Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
A 50-year-old woman had a percutaneous endoscopic gastrostomy (PEG) tube placed after surgery for pharyngeal cancer. After 21 months, the PEG tube was removed due to improvement of per-oral ingestion. She had taken prednisolone for 31 years for systemic lupus erythematosus. The post-PEG fistula did not close spontaneously. The cause of the fistula was slow wound healing and gastrostomy site inflammation due to long-term steroid therapy. We were able to close the fistula with an over-the-scope clipping (OTSC) system. This case suggests that OTSC is useful for closing persistent post-PEG fistulas in patients receiving long-term prednisolone therapy.
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- 2015
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31. Therapeutic or spontaneous Helicobacter pylori eradication can obscure magnifying narrow-band imaging of gastric tumors
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Masaaki Kobayashi, Satoru Hashimoto, Ken-ichi Mizuno, Manabu Takeuchi, Yuichi Sato, Gen Watanabe, Yoichi Ajioka, Motoi Azumi, Kouhei Akazawa, and Shuji Terai
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Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Background and study aims: We previously reported that narrow-band imaging with magnifying endoscopy (NBI-ME) revealed a unique “gastritis-like” appearance in approximately 40 % of early gastric cancers after Helicobacter pylori eradication. Because rates of gastric cancer are increasing in patients with non-persistent infection of H. pylori, we aimed to clarify contribution factors to obscure tumors after therapeutic or spontaneous eradication. Patients and methods: NBI-ME findings were examined retrospectively in 194 differentiated-type adenocarcinomas from H. pylori-negative patients with prior eradication therapy (83 patients) or without prior eradication therapy (72 patients). A gastritis-like appearance under NBI-ME was defined as an orderly microsurface structure and/or loss of clear demarcation with resemblance to the adjacent, non-cancerous mucosa. The correlation of this phenomenon with the degree of atrophic gastritis, determined both histologically in the adjacent mucosa and endoscopically, was evaluated. Results: The tumor-obscuring gastritis-like appearance was observed in 42 % and 23 % of the patients in the H. pylori eradication and non-eradication groups, respectively. The development of this appearance was affected by the histological grade of atrophy (P = 0.003) and intestinal metaplasia (P
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- 2016
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32. Endoscopic Removal of Ingested Dentures and Dental Instruments: A Retrospective Analysis
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Ken-ichi Mizuno, Kazuya Takahashi, Kentaro Tominaga, Yuki Nishigaki, Hiroki Sato, Satoshi Ikarashi, Kazunao Hayashi, Takashi Yamamoto, Yutaka Honda, Satoru Hashimoto, Kenya Kamimura, Manabu Takeuchi, Junji Yokoyama, Yuichi Sato, Masaaki Kobayashi, and Shuji Terai
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Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Background. Dentures and dental instruments are frequently encountered ingested foreign bodies. The aim of the present study was to assess the safety and efficacy of endoscopically removing ingested dental objects. Methods. Twenty-nine consecutive patients with 29 dental objects who were treated at the Niigata University Medical and Dental Hospital from August 2009 to December 2015 were retrospectively reviewed. Characteristics of the patients and the ingested dental objects, the clinical features and findings of radiological imaging tests, and outcomes of endoscopic removal were analyzed. Results. Patients’ mean age was 62.9±21.0 years. The ingested dental objects included 23 dentures (13 crowns, 4 bridges, 4 partial dentures, and 2 other dentures) and 6 dental instruments. Twenty-seven upper gastrointestinal endoscopies and 2 colonoscopies were performed, and their success rates were 92.6% and 100%, respectively. There were 2 cases of removal failure; one case involved an impacted partial denture in the cervical esophagus, and this case required surgical removal. Conclusions. Endoscopic removal of ingested dentures and dental instruments is associated with a favorable success rate and acceptable complications. The immediate intervention and appropriate selection of devices are essential for managing ingested dental objects.
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- 2016
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33. The Combination Therapy of Dissolution Using Carbonated Liquid and Endoscopic Procedure for Bezoars: Pragmatical and Clinical Review
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Kohei Ogawa, Kenya Kamimura, Ken-ichi Mizuno, Yoko Shinagawa, Yuji Kobayashi, Hiroyuki Abe, Yukari Watanabe, Shunsaku Takahashi, Kazunao Hayashi, Junji Yokoyama, Manabu Takeuchi, Masaaki Kobayashi, Satoshi Yamagiwa, Yuichi Sato, and Shuji Terai
- Subjects
Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Bezoars are relatively rare foreign bodies of gastrointestinal tract and often cause ileus and ulcerative lesions in the stomach and subsequent bleeding and perforation due to their size and stiffness. Therefore, the removal of bezoars is essential and recent development of devices, the endoscopic removal procedure, is often applied. However, due to their stiffness, simple endoscopic removal failed in not a few cases, and surgical removal has also been used. Recently, the efficacy of a combination therapy of endoscopic procedure and dissolution using carbonated liquid has been reported. To develop the safe and effective removal procedure, we carefully reviewed a total of 55 reported cases in this study including our 3 additional cases, successfully treated with dissolution with endoscopic fragmentation. In summary, the data showed the efficiency in the combination therapy, treating the larger size of bezoar and reducing the length of hospital stay. To the best of our knowledge, this is the largest pragmatical and clinical review for the combination therapy of dissolution and endoscopic treatment for bezoars. This review should help physicians to manage bezoars more efficiently.
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- 2016
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34. Hand, Foot, and Mouth Disease Caused by Coxsackievirus A6, Japan, 2011
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Tsuguto Fujimoto, Setsuko Iizuka, Miki Enomoto, Katsuhiko Abe, Kazuyo Yamashita, Nozomu Hanaoka, Nobuhiko Okabe, Hiromu Yoshida, Yoshinori Yasui, Masaaki Kobayashi, Yoshiki Fujii, Hiroko Tanaka, Miwako Yamamoto, and Hiroyuki Shimizu
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enterovirus ,hand ,foot ,and mouth disease ,herpangina ,coxsackievirus ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Published
- 2012
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35. Assessment of Gastric Phenotypes Using Magnifying Narrow-Band Imaging for Differentiation of Gastric Carcinomas from Adenomas
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Masaaki Kobayashi, Satoru Hashimoto, Ken Nishikura, Ken-Ichi Mizuno, Manabu Takeuchi, and Yoichi Ajioka
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Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Background. Conventional white-light endoscopy and forceps biopsy are insufficient for definitive diagnosis of gastric adenoma. Immunohistochemical studies have reported an obvious phenotypic difference between adenomas and carcinomas. We investigated the utility of narrow-band imaging with magnifying endoscopy (NBI-ME) for mucin phenotypic assessment to differentiate carcinomas from adenomas. Methods. NBI-ME findings were classified into A, B, and AB types, which revealed papillary, tubular pits and groove microstructures, respectively. To investigate A-B classifications retrospectively, 137 patients (155 lesions) that were diagnosed pretherapeutically with adenoma or borderline lesions by biopsy were enrolled. The mucin phenotype was analyzed immunohistochemically in the first 60 lesions. Results. After endoscopic submucosal dissection, A type and AB type lesions were determined histologically as carcinoma (81/82, 99%). B type lesions were adenoma (29/73, 40%) and carcinoma (44/73, 60%). A or AB type correlated to histological carcinomas (sensitivity 65%, specificity 97%, and accuracy 71%). Mucin phenotypes were gastric or gastrointestinal in A type and AB type carcinomas (31/37, 84%) and intestinal in B type adenomas and carcinomas (21/23, 91%). Conclusions. NBI-ME has the advantage of the assessment of mucin phenotypes in gastric carcinomas and adenomas. The proposed A-B classification is useful, especially for differentiation of gastric or gastrointestinal carcinomas from adenomas.
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- 2014
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36. A Study on Specific Emitter Identification Using Modified Allan Variance and Correlation Coefficient.
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Yan Zhu, Sei Arima, Ryo Hirayama, Masaaki Kobayashi, and Takeshi Kumaki
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- 2021
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37. Tips and Tricks to Improve CNN-based Chest X-ray Diagnosis: A Survey.
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Changhee Han, Takayuki Okamoto, Koichi Takeuchi, Dimitris Katsios, Andrey Grushnikov, Masaaki Kobayashi, Antoine Choppin, Yutaka Kurashina, and Yuki Shimahara
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- 2021
38. Whole genome sequencing and evolutionary analysis of G8P [8] rotaviruses emerging in Japan
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Tung Phan, Masaaki Kobayashi, Koo Nagasawa, Riona Hatazawa, Ngan Thi Kim Pham, Hideaki Miyashita, Satoshi Komoto, Takeshi Tajima, Tuneyoshi Baba, Shoko Okitsu, Pattara Khamrin, Niwat Maneekarn, Hirokazu Kimura, Takeshi Kobayashi, Satoshi Hayakawa, and Hiroshi Ushijima
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Infectious Diseases ,Short Communication ,Virology - Abstract
Unusual DS-1-like intergenogroup reassortant rotaviruses with a bovine-like G8 genotype (DS-1-like G8P [8] rotaviruses) have emerged and rapidly spread in several countries. In this study, the nucleotide sequences of seven human rotavirus G8P [8] strains in 2017 and 2019 in Japan were determined using viral metagenomics. Its genomic constellation (VP7-VP4-VP6-VP1-VP2-VP3-NSP1-NSP2-NSP3-NSP4-NSP5 genes) was defined as G8-P [8]-I2-R2-C2-M2-A2-N2-T2-E2-H2. Our genetic analysis revealed that the Japanese G8P [8] rotavirus strains in 2017 and 2019 were classified into the same lineages G8-5 and P [8]-3, but they were phylogenetically located on separate branches and belonged to distinct clusters. Our study is the first attempt to investigate the evolution of emerging rotavirus G8P [8] in Japan.
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- 2022
39. Endoscopic Curettage of Bone Cysts of the Proximal Humerus
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Masaaki Kobayashi, Isato Sekiya, and Hisaki Aiba
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- 2023
40. Benzalkonium chloride-induced myofibroblastic transdifferentiation of Tenon’s capsule fibroblasts is inhibited by coculture with corneal epithelial cells or by interleukin-10
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Tadahiko Ogata, Manami Ota, Kazuhiro Tokuda, Shinichiro Teranishi, Sho-Hei Uchi, Takuya Yoshimoto, Masaaki Kobayashi, Chiemi Yamashiro, Makoto Hatano, Makiko Wakuta, Atsushige Ashimori, Fumiaki Higashijima, Kazuhiro Kimura, and Yuka Kobayashi
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Tenon Capsule ,Science ,Immunofluorescence ,Article ,Cornea ,Benzalkonium chloride ,Tenon's capsule ,Fibrosis ,medicine ,Humans ,Myofibroblasts ,Eye diseases ,Cells, Cultured ,Corneal epithelium ,Cell Proliferation ,Multidisciplinary ,Molecular medicine ,medicine.diagnostic_test ,Chemistry ,Transdifferentiation ,Epithelial Cells ,Fibroblasts ,medicine.disease ,Molecular biology ,Actins ,Coculture Techniques ,Interleukin-10 ,Interleukin 10 ,medicine.anatomical_structure ,Cell Transdifferentiation ,Trans-Activators ,Medicine ,sense organs ,Benzalkonium Compounds ,Myofibroblast ,medicine.drug ,Signal Transduction - Abstract
Benzalkonium chloride (BAC) is used as a preservative in eyedrops but induces subconjunctival fibrosis that can result in failure of glaucoma surgery. Tenon’s capsule fibroblasts in subconjunctival tissue interact with the corneal epithelium through tear fluid. With the use of a coculture system, we have now investigated the effect of human corneal epithelial (HCE) cells on myofibroblastic transdifferentiation of human Tenon fibroblasts (HTFs) induced by BAC (5 × 10−6%). Immunofluorescence and immunoblot analyses revealed that the BAC-induced expression of α smooth muscle actin (αSMA) in HTFs was suppressed by coculture of these cells with HCE cells (p p p p
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- 2021
41. Inhibition of epithelial–mesenchymal transition in retinal pigment epithelial cells by a retinoic acid receptor-α agonist
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Takuya Yoshimoto, Tadahiko Ogata, Atsushige Ashimori, Masaaki Kobayashi, Kazuhiro Kimura, Sho-Hei Uchi, Makoto Hatano, Chiemi Yamashiro, Makiko Wakuta, Kazuhiro Tokuda, Yuka Kobayashi, Fumiaki Higashijima, and Manami Ota
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0301 basic medicine ,Epithelial-Mesenchymal Transition ,Tetrahydronaphthalenes ,Science ,Retinal Pigment Epithelium ,Smad2 Protein ,Benzoates ,Article ,Focal adhesion ,Mice ,Transforming Growth Factor beta2 ,03 medical and health sciences ,chemistry.chemical_compound ,Medical research ,0302 clinical medicine ,Fibrosis ,medicine ,Animals ,Epithelial–mesenchymal transition ,Phosphorylation ,Eye diseases ,Paxillin ,Cell Proliferation ,Multidisciplinary ,biology ,Interleukin-6 ,Retinoic Acid Receptor alpha ,Muscle, Smooth ,Retinal ,medicine.disease ,Actins ,Cell biology ,Mice, Inbred C57BL ,Fibronectin ,Retinoic acid receptor ,030104 developmental biology ,chemistry ,Trans-Activators ,030221 ophthalmology & optometry ,biology.protein ,Medicine ,Female ,Collagen ,Type I collagen ,Signal Transduction - Abstract
Epithelial–mesenchymal transition (EMT) in retinal pigment epithelial (RPE) cells plays a key role in proliferative retinal diseases such as age-related macular degeneration by contributing to subretinal fibrosis. To investigate the potential role of retinoic acid receptor-α (RAR-α) signaling in this process, we have now examined the effects of the RAR-α agonist Am580 on EMT induced by transforming growth factor-β2 (TGF-β2) in primary mouse RPE cells cultured in a three-dimensional type I collagen gel as well as on subretinal fibrosis in a mouse model. We found that Am580 inhibited TGF-β2-induced collagen gel contraction mediated by RPE cells. It also attenuated the TGF-β2-induced expression of the mesenchymal markers α-smooth muscle actin, fibronectin, and collagen type I; production of pro-matrix metalloproteinase 2 and interleukin-6; expression of the focal adhesion protein paxillin; and phosphorylation of SMAD2 in the cultured RPE cells. Finally, immunofluorescence analysis showed that Am580 suppressed both the TGF-β2-induced translocation of myocardin-related transcription factor-A (MRTF-A) from the cytoplasm to the nucleus of cultured RPE cells as well as subretinal fibrosis triggered by laser-induced photocoagulation in a mouse model. Our observations thus suggest that RAR-α signaling inhibits EMT in RPE cells and might attenuate the development of fibrosis associated with proliferative retinal diseases.
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- 2021
42. Midterm results after seamless patch mitral reconstruction
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Soh Hosoba, Toshiaki Ito, Makoto Mori, Riku Kato, Masaaki Kobayashi, Yuji Nakai, and Yoshihiro Morishita
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Pulmonary and Respiratory Medicine ,Surgery - Abstract
Some pathologies, including infective endocarditis or sclerotic changes of the mitral leaflet, make the conventional mitral valve repair challenging. Our previously described technique for reconstruction with a seamless pericardial patch makes the repair feasible in some of such difficult pathologies. However, the extent of mitral leaflet segments that could be safely repaired using this technique remains unknown. We investigated the association between the midterm outcome and the extent of mitral leaflet segments replaced by a pericardial patch.From January 2009 to January 2022, patients who underwent mitral valve repair with the seamless 1-patch reconstruction technique were included. The glutaraldehyde-treated pericardium was trimmed and anchored at the papillary muscle. The edge was sewn to the leaflet and the annulus.A total of 49 patients (aged 60 ± 15 years) underwent mitral valve repair with this technique. The totally endoscopic approach was used in 27 patients (55%). No patient's repair was converted to valve replacement. No operative mortality or disabling stroke was observed during the early postoperative period. In the midterm follow-up, redo surgery was required in 9 patients (18%). Freedom from mitral valve reintervention rates at 1, 5, and 10 years were 84%, 82%, and 82% for all patients, respectively. Freedom from reoperation at 5 years was 100%, 92%, and 46% for commissural lesion, 1- to 2-segment involvement, and 3-segment involvement, respectively. There was a significant difference among the 3 groups with regard to mitral valve reoperation rate (Mitral valve seamless patch reconstruction provides excellent midterm results if applied to commissural lesions or lesions involving up to 2 segments.
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- 2022
43. Distinct epigenetic signatures between adult-onset and late-onset depression
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Shigeo Miyata, Ayumi Kobayashi, Yoshihiko Hamamoto, Hiroyuki Ogihara, Shusaku Uchida, Masaaki Kobayashi, Tomoe Seki, Yoshifumi Watanabe, Shin Nakagawa, Masato Fukuda, Masahiko Mikuni, Kenichiro Harada, Chong Chen, Hirotaka Yamagata, and Koji Matsuo
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0301 basic medicine ,Oncology ,Epigenomics ,Genetic Markers ,Male ,medicine.medical_specialty ,Science ,Bisulfite sequencing ,Late onset ,Article ,Epigenesis, Genetic ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Medicine ,Humans ,Epigenetics ,Depression (differential diagnoses) ,Aged ,Depressive Disorder, Major ,Multidisciplinary ,DNA methylation ,business.industry ,Depression ,Diagnostic markers ,Methylation ,Middle Aged ,medicine.disease ,030104 developmental biology ,CpG site ,Major depressive disorder ,Female ,business ,030217 neurology & neurosurgery - Abstract
The heterogeneity of major depressive disorder (MDD) is attributed to the fact that diagnostic criteria (e.g., DSM-5) are only based on clinical symptoms. The discovery of blood biomarkers has the potential to change the diagnosis of MDD. The purpose of this study was to identify blood biomarkers of DNA methylation by strategically subtyping patients with MDD by onset age. We analyzed genome-wide DNA methylation of patients with adult-onset depression (AOD; age ≥ 50 years, age at depression onset
- Published
- 2021
44. Endodermal Cyst with a Non-enhancing Nodule in the Quadrigeminal Cistern Developed in an Octogenarian
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Yasuhiro Aida, Katsuyoshi Miyashita, Yu Shimizu, Masaaki Kobayashi, Yasuo Tohma, Tomohiro Kitabayashi, and Nozomu Oikawa
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medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Adhesion (medicine) ,Magnetic resonance imaging ,Nodule (medicine) ,medicine.disease ,Cerebellopontine angle ,Hydrocephalus ,Lesion ,03 medical and health sciences ,0302 clinical medicine ,030220 oncology & carcinogenesis ,embryonic structures ,parasitic diseases ,medicine ,Surgery ,Cyst ,Neurology (clinical) ,Radiology ,Differential diagnosis ,medicine.symptom ,business ,030217 neurology & neurosurgery - Abstract
Background Intracranial endodermal cysts are congenital lesions that generally develop in the cerebellopontine angle and ventral brainstem of the posterior fossa, whereas endodermal cysts in the quadrigeminal cistern are very rare. We report a rare case of an endodermal cyst in the quadrigeminal cistern with a non-enhancing nodule that developed in patient over 80 years of age. Case Description An 85-year-old man presented to our hospital with progressing gait disturbance and urinary incontinence. Preoperative images showed a cystic mass lesion with a nodule in the quadrigeminal cistern and hydrocephalus. There was no enhanced portion in the lesion, and the intensity of the cyst on magnetic resonance imaging revealed a high protein concentration. Subtotal resection was performed due to the adhesion of the cyst to the brainstem. It was diagnosed as an endodermal cyst. The postoperative course was uneventful, and hydrocephalus improved. Conclusions This is a rare case of an intracranial endodermal cyst in terms of location and age of onset compared with previous reports. This case demonstrates that endodermal cysts should be considered as a differential diagnosis for lesions in the quadrigeminal cistern with high protein concentration in the cyst and nodule representing chronic inflammation, regardless of enhancing effects.
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- 2020
45. Upper-Limb Power-Assist Control for Agriculture Load Lifting.
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Eiichi Yagi, Daisuke Harada, and Masaaki Kobayashi
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- 2009
- Full Text
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46. Five Cases of Epidemic Keratoconjunctivitis Due to Human Adenovirus Type 85 in Fukushima, Japan
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Hisatoshi Kaneko, Hiroshi Hatano, Masami Konagaya, Nozomu Hanaoka, Tsuguto Fujimoto, Masaaki Kobayashi, Tetsuju Sekiryu, Hisashi Nakagawa, and Kazufumi Ikuta
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Adult ,Male ,Microbiology (medical) ,business.industry ,Adenoviruses, Human ,Keratoconjunctivitis ,General Medicine ,Middle Aged ,Polymerase Chain Reaction ,Virology ,Disease Outbreaks ,Epidemic Keratoconjunctivitis ,Community-Acquired Infections ,Infectious Diseases ,Japan ,Humans ,Medicine ,Female ,Databases, Nucleic Acid ,business - Published
- 2020
47. Distribution of rotavirus genotypes in Japan from 2015 to 2018: Diversity in genotypes before and after introduction of rotavirus vaccines
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Satoshi Hayakawa, Masashi Mizuguchi, Tsuneyoshi Baba, Niwat Maneekarn, Sayaka Takanashi, Masaaki Kobayashi, Nusrat Khandoker, Kumiko Sugita, Hiroshi Ushijima, Sheikh Ariful Hoque, Hideaki Kikuta, Shoko Okitsu, Pattara Khamrin, Atsuko Yamamoto, Aksara Thongprachum, and Shuichi Nishimura
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Rotavirus ,Veterinary medicine ,Genotype ,Biology ,medicine.disease_cause ,Rotavirus Infections ,Feces ,03 medical and health sciences ,0302 clinical medicine ,Japan ,030225 pediatrics ,medicine ,Humans ,Outpatient clinic ,030212 general & internal medicine ,Child ,Genotyping ,Phylogeny ,General Veterinary ,General Immunology and Microbiology ,Rotavirus Vaccines ,Public Health, Environmental and Occupational Health ,Infant ,Acute gastroenteritis ,Infectious Diseases ,Molecular Medicine ,Detection rate - Abstract
Background Diversity in group A rotavirus (RVA) strains after introduction of RV-vaccines remains an emerging concern worldwide. In this study, we investigated the prevalence and distribution of RVA genotypes in Japanese children with acute gastroenteritis (AGE) from 2015 to 2018. In addition, a comparison of the genotypes in pre-vaccination (2006–2012) and post-vaccination (2012–2018) periods was conducted to understand the impact of these vaccines on genotype distribution. Methods Fecal samples were collected regularly from outpatient clinics in six localities: Hokkaido, Tokyo, Shizuoka, Osaka, Kyoto, and Saga. RVA were screened and genotyped by RT-PCR and sequence-based genotyping. Results During the period 2015–2018, RVA was detected in 307 (19.7%) samples out of 1557 specimens: 29.9% (95% CI: 25.8% to 34.3%), 17.9% (95% CI: 14.7% to 21.5%), and 13% (95% CI: 10.3% to 16.0%) were detected RVA-positive in 2015–2016, 2016–2017 and 2017–2018, respectively. The average detection of RVA in pre-vaccination (2006–2012) and post-vaccination (2012–2018) era remained almost similar (18%-20%). The G2P[4]I2 (52.1%, 95% CI: 43.5%-60.6%) remained the most common genotype in 2015–2016, whereas G8P[8]I2 (55.9%, 95% CI: 45.2%-66.2%) dominated in 2016–2017. In 2017–2018, G9P[8]I2 (42.0%, 95% CI: 30.5%-53.9%) prevailed, followed by G9P[8]I1 (23.0%, 95% CI: 14.0%-34.2%). The detection rate of some common genotypes of pre-vaccination era like G1P[8] and G3P[8] has been reduced after introduction of RV-vaccine, whereas genotypes that were sporadic before the introduction of vaccines like G2P[4], G2P[8], G9P[8] and G8P[8] were emerged/reemerged in post-vaccination period. Conclusions Our study presented the diversity in circulating RVA genotypes in Japan before and after introduction of RV-vaccines. Sudden emergence of DS-1-like (I2) unusual strains in post-vaccination era remains alarming. Continuous monitoring of RVA genotypes is therefore indispensable to refine future vaccine strategy.
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- 2020
48. RAD-seq-Based High-Density Linkage Map Construction and QTL Mapping of Biomass-Related Traits in Sorghum using the Japanese Landrace Takakibi NOG
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Everlyne A Omollo, Masaaki Kobayashi, Wataru Sakamoto, Norikazu Ohnishi, Masaru Fujimoto, Hideki Takanashi, Hiromi Kajiya-Kanegae, Hiroyoshi Iwata, Nobuhiro Tsutsumi, Toshiaki Kozuka, Motoyuki Ishimori, Makoto Kusaba, Fiona Wacera W, Michiharu Nakano, and Kentaro Yano
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0106 biological sciences ,0301 basic medicine ,Physiology ,Quantitative Trait Loci ,Population ,Plant Science ,Quantitative trait locus ,Polymorphism, Single Nucleotide ,01 natural sciences ,Genome ,DNA sequencing ,03 medical and health sciences ,Quantitative Trait, Heritable ,Genetic linkage ,Biomass ,education ,Sorghum ,Genetics ,Brix ,Genetic diversity ,education.field_of_study ,biology ,Chromosome Mapping ,Genetic Variation ,food and beverages ,Sequence Analysis, DNA ,Cell Biology ,General Medicine ,biology.organism_classification ,030104 developmental biology ,Genome, Plant ,010606 plant biology & botany - Abstract
Sorghum [Sorghum bicolor (L.) Moench] grown locally by Japanese farmers is generically termed Takakibi, although its genetic diversity compared with geographically distant varieties or even within Takakibi lines remains unclear. To explore the genomic diversity and genetic traits controlling biomass and other physiological traits in Takakibi, we focused on a landrace, NOG, in this study. Admixture analysis of 460 sorghum accessions revealed that NOG belonged to the subgroup that represented Asian sorghums, and it was only distantly related to American/African accessions including BTx623. In an attempt to dissect major traits related to biomass, we generated a recombinant inbred line (RIL) from a cross between BTx623 and NOG, and we constructed a high-density linkage map based on 3,710 single-nucleotide polymorphisms obtained by restriction-site-associated DNA sequencing of 213 RIL individuals. Consequently, 13 fine quantitative trait loci (QTLs) were detected on chromosomes 2, 3, 6, 7, 8 and 9, which included five QTLs for days to heading, three for plant height (PH) and total shoot fresh weight and two for Brix. Furthermore, we identified two dominant loci for PH as being identical to the previously reported dw1 and dw3. Together, these results corroborate the diversified genome of Japanese Takakibi, while the RIL population and high-density linkage map generated in this study will be useful for dissecting other important traits in sorghum.
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- 2020
49. Progressively Enlarged Convexity Arachnoid Cysts in Elderly Patients: A Report of 2 Cases
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Masahiro Oishi, Yasuhiko Hayashi, Ryouken Kimura, Masashi Kinoshita, Masaaki Kobayashi, Mitsutoshi Nakada, and Yasuo Sasagawa
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medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Magnetic resonance imaging ,medicine.disease ,Shunt (medical) ,03 medical and health sciences ,0302 clinical medicine ,medicine.anatomical_structure ,Cerebrospinal fluid ,Ventricle ,030220 oncology & carcinogenesis ,Memory disturbance ,medicine ,Surgery ,Cyst ,Arachnoid Membrane ,Neurology (clinical) ,Radiology ,Subdural space ,business ,030217 neurology & neurosurgery - Abstract
Background Generally, enlargement of arachnoid cysts (ACs) has been found mostly in cases occurring during early childhood. Therefore, progressively enlarged ACs found to be symptomatic in elderly patients are extremely rare, and the mechanisms have remained unexplored. Case Description Our first patient was a 72-year-old woman with memory disturbance, who had presented with a large cyst beneath the right temporal convexity 9 years previously. The annual follow-up magnetic resonance imaging (MRI) studies had revealed that the cyst had progressively enlarged. In addition, her memory disturbance had become advanced. Endoscopic cyst fenestration was performed between the cyst and lateral ventricle, resulting in a reduction of her symptoms. Our second patient was a 79-year-old woman with unsteadiness, who had presented with a large cyst under the right parietal convexity 6 years previously. The annual follow-up MRI studies had shown that the cyst had gradually enlarged. She subsequently developed left hemiparesis. Because the pyramidal tract was located between the cyst and ventricle, a cyst–ventricle shunt was placed to allow the cystic fluid into the lateral ventricle, with complete resolution of her symptoms. In both cases, MRI showed obliteration of the subdural spaces around the cysts. Endoscopic observations revealed that the arachnoid membrane was lined under the surrounding brain, leading to the diagnosis of an AC. Conclusion The establishment of stable communication between a cyst and the normal cerebrospinal fluid space is important to treat symptomatic ACs characterized by progressive enlargement, even in elderly patients. The 1-way entry of the cerebrospinal fluid into the cyst and the closure of the surrounding subdural space might result in AC enlargement internally.
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- 2020
50. <scp>Plant Omics Data Center</scp> and <scp>CATchUP</scp> : web databases for effective gene mining utilizing public <scp>RNA</scp> ‐ <scp>S</scp> eq‐based transcriptome data
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Toru Kudo, Matt Shenton, Yukino Nakamura, Hajime Ohyanagi, Kentaro Yano, and Masaaki Kobayashi
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Omics data ,Transcriptome ,Center (algebra and category theory) ,RNA-Seq ,Computational biology ,Biology ,Gene mining - Published
- 2019
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