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2 results on '"Marycin"'

1. Alterations of RNA Metabolism by Proteomic Analysis of Breast Cancer Cells Exposed to Marycin: A New Optically Active Porphyrin

Author

Arnaldo Pinelli, Paola Perego, Maida De Bortoli, Gabriella Tedeschi, Cristina Corno, Elena Taverna, Elisa Maffioli, Emilio Ciusani, Silvio Trivulzio, and Italia Bongarzone

Subjects
Proteomics, Porphyrins, RNA Splicing, Apoptosis, Breast Neoplasms, Mitochondrion, Tandem mass spectrometry, Flow cytometry, Marycin, Tandem Mass Spectrometry, anti-proliferative effect, Settore BIO/10 - Biochimica, Cell Line, Tumor, Organelle, Fluorescence microscope, medicine, Humans, LC-MS/MS, Chromatography, High Pressure Liquid, RNA metabolism, medicine.diagnostic_test, Chemistry, peroxisomes, Cell Cycle Checkpoints, General Medicine, Peroxisome, Cell biology, Gene Expression Regulation, Neoplastic, Hematoporphyrins, RNA splicing, Proteome, RNA, MDA-MB-231 breast cancer cells, Female, Reactive Oxygen Species, porphyrin
Abstract

Objective: Marycin is a porphyrin-type compound synthetically modified to spontaneously release fluorescence. This study is aimed at understanding possible mechanisms that could account for the antiproliferative effects observed in marycin. A proteomic approach was used to identify molecular effects. The proteome of proliferating MDA-MB-231 breast cancer cells was compared with that of marycin-treated cells. Methods: Label-free proteomic analysis by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was used to reveal changes in protein expression and fluorescence microscopy and flow cytometry were used to detect subcellular organelle dysfunctions. Results: The bioinformatic analysis indicated an enhancement of the expression of proteins remodeling RNA splicing and more in general, of RNA metabolism. Marycin did not localize into the mitochondria and did not produce a dramatic increase of ROS levels in MDA-MB-231 cells. Marycin stained organelles probably peroxisomes. Conclusions: The results could support the possibility that the peroxisomes are involved in cell response to marycin.

Published
2019

2. Alterations of RNA Metabolism by Proteomic Analysis of Breast Cancer Cells Exposed to Marycin: A New Optically Active Porphyrin.

Author

Taverna E, De Bortoli M, Maffioli E, Corno C, Ciusani E, Trivulzio S, Pinelli A, Tedeschi G, Perego P, and Bongarzone I

Subjects
Apoptosis drug effects, Breast Neoplasms metabolism, Breast Neoplasms pathology, Cell Cycle Checkpoints drug effects, Cell Line, Tumor, Chromatography, High Pressure Liquid, Female, Hematoporphyrins chemistry, Humans, Porphyrins chemistry, RNA Splicing drug effects, Reactive Oxygen Species metabolism, Tandem Mass Spectrometry, Gene Expression Regulation, Neoplastic drug effects, Hematoporphyrins pharmacology, Porphyrins pharmacology, Proteomics methods, RNA metabolism
Abstract

Objective: Marycin is a porphyrin-type compound synthetically modified to spontaneously release fluorescence. This study is aimed at understanding possible mechanisms that could account for the antiproliferative effects observed in marycin. A proteomic approach was used to identify molecular effects. The proteome of proliferating MDA-MB-231 breast cancer cells was compared with that of marycin-treated cells., Methods: Label-free proteomic analysis by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was used to reveal changes in protein expression and fluorescence microscopy and flow cytometry were used to detect subcellular organelle dysfunctions., Results: The bioinformatic analysis indicated an enhancement of the expression of proteins remodeling RNA splicing and more in general, of RNA metabolism. Marycin did not localize into the mitochondria and did not produce a dramatic increase of ROS levels in MDA-MB-231 cells. Marycin stained organelles probably peroxisomes., Conclusions: The results could support the possibility that the peroxisomes are involved in cell response to marycin., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)

Published
2019
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