1. Correlation of intratumoral and peritumoral expression of endoglin (CD105) and clinicopathological factors in patients undergoing surgery for rectal carcinoma
- Author
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Martinović, Željko, Kovač, Dražen, Tomić, Snježana, Družijanić, Nikica, and Jonjić, Nives
- Subjects
Pathologic Neovascularization ,Rectal Neoplasms ,Carcinogenesis ,Transformirajući faktor rasta beta ,BIOMEDICINE AND HEALTHCARE. Clinical Medical Sciences. Pathology ,Smad proteini ,Smad Proteins ,Karcinogeneza ,BIOMEDICINA I ZDRAVSTVO. Kliničke medicinske znanosti. Patologija ,udc:616(043.3) ,Transforming Growth Factor beta ,Rektalne novotvorine ,Pathology. Clinical medicine ,CD antigeni ,Patologija. Klinička medicina ,Patološka neovaskularizacija ,CD Antigens - Abstract
Uvod: Uloga endoglina i prognostičko značenje angiogeneze u Dukes B karcinomu rektuma još uvijek su nedovoljno neistraženi. Hipoteza i ciljevi: Ekspresija endoglina i mikrovaskularna gustoća su prognostički čimbenici rizika kod bolesnika s Dukes B karcinomom rektuma. Cilj ovog istraživanja je ispitati ekspresiju endoglina (CD105) u reseciranim uzorcima karcinoma rektuma i odnos između mikrovaskularne gustoće (MVD), kliničkopatoloških čimbenika, preživljenja bolesnika i recidiva tumora. Materijali i metode: U istraživanje je uključeno 95 uzoraka primarnog adenokarcinoma rektuma, 67 uzoraka korespondentne susjedne normalne sluznice i 73 uzorka udaljene normalne sluznice uzetih od kirurškog preparata reseciranog tumora rektuma. Uzorci su uklopljeni u parafin i imunohistokemijski obojeni na CD105 endotelni antigen te je provedeno brojanje malih krvnih žila. Za točno mjerenje CD105-MVD korišten je računalno objedinjeni sustav Alphelys Spot Browser 2. Rezultati: CD105-MVD u tumoru bila je značajno veća u usporedbi s korespondentnom susjednom sluznicom (P, Background: The role endoglin in the Dukes B rectal cancer still unexplored. The aim of this study was to examine the expression of endoglin (CD105) in resected rectal cancer and evaluate the relationship between microvessels density (MVD), clinicopathological factors and survival. Hypothesis and Objectives: Endoglin expression and microvessel density are prognostic risk factors in patients with Dukes B rectal cancer. The aim of this study was to determine the endoglin expression in resected specimens of rectal cancer and the relationship between microvessel density (MVD), clinical and pathological factors, patients survival and tumor recurrence. Materials and Method: The study included 95 primary rectal adenocarcinomas, corresponding 67 adjacent and 73 distant normal mucosa specimens from surgical resection samples. Tumor specimens were paraffin-embedded and immunohistochemical staining for the CD105 endothelial antigen was performed to count CD105-MVD. For exact measurement of the CD105-MVD used a computer-integrated system Alphelys Spot Browser 2. Results: The intratumoral CD105-MVD was significantly higher compared with corresponding adjacent mucosa (P
- Published
- 2023