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An abstract of the medical research "THERAPEUTIC DRUG MONITORING (TDM) OF GENTAMICIN IN CRITICALLY ILL NEONATES," by Iva Selke-Krulichova, Jirina Martinkova, Pavla Pokorna and Jiri Zahora is presented.

An abstract of the medical research "ARGININE METABOLISM IN A RAT MODEL OF BRONCHIAL ASTHMA," by Zuzana Havlinova, Jaroslav Chladek, Stanislav Micuda, Jana Prokopova and Jirina Martinkova is presented.

An abstract of the medical research "INFLUENCE OF AZITHROMYCIN ON HEPATOBILIARY AND RENAL EXCRETION OF METHOTREXATE IN RATS ," by Jolana Cermanova, Stanislav Micuda, Eva Brcakova, Leos Fuksa, Milos Hroch, Jaroslav Chladek, Jitka Hajkova, Halka Lotkova, Jirina Martinkova, Zuzana Cervinkova and Frantisek Staud is presented.

An abstract of the medical research "CHANGES IN CARDIAC TROPONIN T DURING ACUTE AND CHRONIC CHOLESTASIS IN RATS," by Stanislav Micuda, Michaela Adamcova, Eva Brcakova, Leos Fuksa, Jolana Cermanova, Jitka Hajkova, Gabriela Kolouchova, Jirina Martinkova and Frantisek Staud is presented.

An abstract of the medical research "A RANDOMIZED CROSS-OVER STUDY OF FOLIC ACID SUPPLEMENTATION DURING THE INITIAL PHASE OF PSORIASIS TREATMENT WITH METHOTREXATE," by Jaroslav Chladek, Jaroslava Vaneckova, Marie Simkova, Milos Hroch, Jirina Martinkova and Jiri Grim is presented.

Discusses the use of human hepatocytes cultured in vitro in the study of hepatocytic properties. Comparison of the hepatocytes in situ and in vitro; Analysis of the expression levels and distribution patterns of MRP2 and MDR1; Difference in the expression of hepatocytes.

Resistance to anti-cancer drugs is a well recognized problem and very often it is responsible for failure of the cancer treatment. In this study, the proteome alterations associated with the development of acquired resistance to cyclin-depedent kinases inhibitor bohemine, a promising anti-cancer drug, were analyzed with the primary aim of identifying potential targets of resistance within the cell that could pave a way to selective elimination of specific resistant cell types. A model of parental susceptible CEM T-lymphoblastic leukemia cells and its resistant counterpart CEM-BOH was used and advanced 2-D liquid chromatography was applied to fractionate cellular proteins. Differentially expressed identified proteins were further verified using immunoblotting and immunohistochemistry. Our study has revealed that Rho GDP-dissociation inhibitor 2, Y-box binding protein 1, and the HSP70/90 organizing protein have a critical role to play in resistance to cyclin-depedent kinases inhibitor. The results indicated not only that quantitative protein changes play an important role in drug-resistance, but also that there are various other parameters such as truncation, post-translational modification(s), and subcellular localization of selected proteins. Furthermore, these proteins were validated for their roles in drug resistance using different cell lines resistant to diverse representatives of anti-cancer drugs such as vincristine and daunorubicin.