21 results on '"Martina De Angelis"'
Search Results
2. Functionalized Magnetic Nanoparticles as Catalysts for Enantioselective Henry Reaction
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Carla Sappino, Ludovica Primitivo, Martina De Angelis, Marzia Oneto Domenici, Andrea Mastrodonato, Ilaria Ben Romdan, Chiara Tatangelo, Lorenza Suber, Luciano Pilloni, Alessandra Ricelli, and Giuliana Righi
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Chemistry ,QD1-999 - Published
- 2019
- Full Text
- View/download PDF
3. Asymmetric Hydroarylation Reactions Catalyzed by Transition Metals: Last 10 Years in a Mini Review
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Martina De Angelis, Antonia Iazzetti, Andrea Serraiocco, and Alessia Ciogli
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asymmetric hydroarylation ,transition metal ,asymmetric catalysis ,Chemical technology ,TP1-1185 ,Chemistry ,QD1-999 - Abstract
Hydroarylation reactions play a pivotal role in organic chemistry due to their versatility and efficiency. In the last 10 years, the scientific production around this reaction has been very high, but in its asymmetric version, the results are less. In this mini review, selected literature examples are considered to draw attention to directions of the asymmetric hydroarylation reaction mediated by transition metal catalysts. The selected works were grouped in two main sections. In the first, we reported examples relating the narrower definition of hydroarylation, namely the metal-catalyzed processes where inactivated aryl moiety undergoes a direct functionalization via insertion of an unsaturated compound. In the second part, hydroarylation reactions take place with the use of pre-activated aryl substrates, usually aryl-iodides or aryl-boronated.
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- 2022
- Full Text
- View/download PDF
4. Role of Some Food-Grade Synthesized Flavonoids on the Control of Ochratoxin A in Aspergillus carbonarius
- Author
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Alessandra Ricelli, Martina De Angelis, Ludovica Primitivo, Giuliana Righi, Carla Sappino, and Roberto Antonioletti
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flavones ,aromatic hydroxylation ,Aspergillus carbonarius ,ochratoxin A ,lipoxygenase ,Organic chemistry ,QD241-441 - Abstract
Ochratoxin A (OTA) is a mycotoxin with a serious impact on human health. In Mediterranean countries, the black Aspergilli group, in particular Aspergillus carbonarius, causes the highest OTA contamination. Here we describe the synthesis of three polyphenolic flavonoids: 5-hydroxy-6,7-dimethoxy-flavone (MOS), 5,6-dihydroxy-7-methoxy-flavone (NEG), and 5,6 dihydroxy-flavone (DHF), as well as their effect on the prevention of OTA biosynthesis and lipoxygenase (LOX) activity in A. carbonarius cultured in a conducive liquid medium. The best control effect on OTA biosynthesis was achieved using NEG and DHF. In fungal cultures treated with these compounds at 5, 25, and 50 μg/mL, OTA biosynthesis significantly decreased throughout the 8-day experiment. NEG and DHF appear to have an inhibiting effect also on the activity of LOX, whereas MOS, which did not significantly inhibit OTA production, had no effect on LOX activity. The presence of free hydroxyls in catecholic position in the molecule appears to be a determining factor for significantly inhibiting OTA biosynthesis. However, the presence of a methoxy group in C-7 in NEG could slightly lower the molecule’s reactivity increasing OTA inhibition by this molecule at 5 μg/mL. Polyphenolic flavonoids present in edible plants may be easily synthesized and used to control OTA biosynthesis.
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- 2019
- Full Text
- View/download PDF
5. Antigravitropic PIN polarization maintains non-vertical growth in lateral roots
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Suruchi Roychoudhry, Katelyn Sageman-Furnas, Chris Wolverton, Peter Grones, Shutang Tan, Gergely Molnár, Martina De Angelis, Heather L. Goodman, Nicola Capstaff, James P. B. Lloyd, Jack Mullen, Roger Hangarter, Jiří Friml, and Stefan Kepinski
- Published
- 2023
- Full Text
- View/download PDF
6. Synthesis of 3-substituted 2,3-dihydropyrazino[1,2-a]indol-4(1H)-ones by sequential reactions of 2-indolylmethyl acetates with α-amino acids
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Antonella Goggiamani, Antonio Arcadi, Alessia Ciogli, Martina De Angelis, Stefano Dessalvi, Giancarlo Fabrizi, Federica Iavarone, Antonia Iazzetti, Alessio Sferrazza, and Roberta Zoppoli
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Sequential Reactions. Annulation Reactions. Indole-2-ylmethylacetates. 2 ,Sequential Reactions. Annulation Reactions. Indole-2-ylmethylacetates. 2,3-Dihydropyrazino[1,2-a]indol-4(1H)-ones ,General Chemical Engineering ,General Chemistry ,2-a]indol-4(1H)-ones ,3-Dihydropyrazino[1 - Abstract
The synthesis of 2,3-dihydropyrazino[1,2-a]indol-4(1H)-ones through the in situ generation of 2-methide-2H-indole intermediate I starting from 2-indolylmethyl acetates under basic conditions/nucleophilic Michael addition/cyclization cascade reaction.
- Published
- 2023
7. Silver thiolate nanoclusters as support for chiral ligands: application in heterogeneous phase asymmetric catalysis
- Author
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Ludovica Primitivo, Martina De Angelis, Andrea Necci, Federica Di Pietro, Alessandra Ricelli, Daniela Caschera, Luciano Pilloni, Lorenza Suber, and Giuliana Righi
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General Engineering ,General Materials Science ,Bioengineering ,General Chemistry ,Atomic and Molecular Physics, and Optics - Abstract
A nanocatalyst with chiral amino alcohol catalytic function was efficiently employed in the Henry reaction, easily recovered by centrifugation and reused without loss of activity.
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- 2023
8. Arabidopsis lateral shoots display two distinct phases of growth angle control
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Martina De Angelis and Stefan Kepinski
- Abstract
Shoot growth angle is a fundamental determinant of plant form. In their later development, lateral branches maintain gravitropic setpoint angles (GSAs) in which growth is set and maintained relative to gravity. The typically non-vertical GSAs are the product of an auxin-dependent antigravitropic offset that counteracts underlying gravitropic response in the branch (Roychoudhryet al., 2013). Here we describe an earlier phase of branch development in which the young lateral shoot grows rootward, independently of gravity, promoting a spreading growth habit. In normal development, this phase of growth is terminated with the onset of the GSA programme, with branches then growing upwards to assume their mature form. The biophysical basis of the early rootward phase of branch growth can be traced back to greater cell proliferation on the upper, adaxial side that upon expansion, drives asymmetric growth. Our data indicate that cytokinin is involved in this process and that the transcription factor TCP1 is an important regulator of lateral shoot adaxial identity and differential ad-abaxial cell proliferation.
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- 2023
9. Design of a fluorescent and clickable Ag38(SRN3)24 nanocluster platform: synthesis, modeling and self-assembling
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Martina De Angelis, Gaetano Campi, Luciano Pilloni, Mauro Satta, Lorenza Suber, Alessandro Fortunelli, Amedeo Palma, Luca Sementa, Giuliana Righi, Ludovica Primitivo, Alessandra Del Giudice, and Daniela Caschera
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Materials science ,XRD ,nanoclusters ,UV-vis ,Bioengineering ,Nanotechnology ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Catalysis ,Nanoclusters ,silver ,General Materials Science ,Clickable ,Small-angle X-ray scattering ,theoretical modeling ,General Engineering ,SAXS ,General Chemistry ,fluorescence spectroscopy ,021001 nanoscience & nanotechnology ,Fluorescence ,Atomic and Molecular Physics, and Optics ,0104 chemical sciences ,Surface modification ,0210 nano-technology ,Biosensor ,Stoichiometry ,ATR-FTIR - Abstract
Fluorescent atomically precise Ag38(11-azido-2-ol-undecane-thiolate)24 nanoclusters are easily prepared using sodium ascorbate as a "green"reducer and are extensively characterized by way of elemental analyses, ATR-FTIR, XRD, SAXS, UV-vis, fluorescence spectroscopies, and theoretical modeling. The fluorescence and the atomically determined stoichiometry and structure, the facile and environmentally green synthesis, together with the novel presence of terminal azido groups in the ligands which opens the way to "click"-binding a wide set of molecular species, make Ag38(11-azido-2-ol-undecane-thiolate)24 nanoclusters uniquely appealing systems for biosensing, recognition and functionalization in biomedicine applications and in catalysis. This journal is
- Published
- 2021
10. Preparation of a high-density vinyl silica gel to anchor cysteine via photo-click reaction and its applications in hydrophilic interaction chromatography
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Alessia Ciogli, Fabio Buonsenso, Noemi Proietti, Giulia Mazzoccanti, Simone Manetto, Andrea Calcaterra, Martina De Angelis, and Francesco Gasparrini
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Organic Chemistry ,Horizontal polymerization ,Photo-click reaction ,Silica characterization ,Solid-state NMR ,Vinyl silica gel ,Silica Gel ,General Medicine ,Silanes ,Silicon Dioxide ,Biochemistry ,Analytical Chemistry ,Vinyl silica gel Horizontal polymerization Silica characterization Solid-state NMR Photo-click reaction ,Click Chemistry ,Cysteine ,Hydrophobic and Hydrophilic Interactions ,Chromatography, Liquid - Abstract
Modification of surface silanols is a topic of interest in the preparation of organo-functionalized silica particles. Herein, two novel contributions, mainly focused on separation science, were presented: i) the horizontal polymerization on silica surface by using the vinyl-triacetoxy silane and ii) a preparation of cysteine-based stationary phase via photo-click thiol-ene coupling. In the first derivatization step, the vinyl-triacetoxy silane was employed instead of conventional trichloro vinyl one. The one-step synthetic procedure needed imidazole as an activating agent in addition to the silica hydration. Modified silica particles offer a high loading of vinyl fragments and an extensive passivation of silanols such as to not require a subsequent end-capping procedure. The structural morphology of media was deeper characterized by combining infrared spectroscopy, solid-state nuclear magnetic resonance, and elemental analysis. A first application, the photo-click cysteine-based material was prepared by photo-click reaction and the stationary phase was employed in the separation of some conventional targets by hydrophilic interaction chromatography.
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- 2022
11. Reactivity of Indolylmethylacetates with N, O, and S Soft Nucleophiles: Evidence of 2-Alkylideneindolenines and 3-Alkylideneindoleninium Generation by ESI-MS and IRMPD Spectroscopy
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Antonio Arcadi, Giel Berden, Alessia Ciogli, Davide Corinti, Maria Elisa Crestoni, Martina De Angelis, Giancarlo Fabrizi, Antonella Goggiamani, Antonia Iazzetti, Federico Marrone, Vincenzo Marsicano, Jos Oomens, and Andrea Serraiocco
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FELIX Molecular Structure and Dynamics ,Carbinols ,Synthetic methods ,Indole-2-methides ,Settore CHIM/06 - CHIMICA ORGANICA ,Nitrogen heterocycles ,Organic Chemistry ,IRMPD ,Physical and Theoretical Chemistry - Abstract
Contains fulltext : 284404.pdf (Publisher’s version ) (Closed access)
- Published
- 2022
12. Functionalized Magnetic Nanoparticles as Catalysts for Enantioselective Henry Reaction
- Author
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Ludovica Primitivo, Marzia Oneto Domenici, Lorenza Suber, Ilaria Ben Romdan, Martina De Angelis, Alessandra Ricelli, Giuliana Righi, Andrea Mastrodonato, Carla Sappino, Luciano Pilloni, Chiara Tatangelo, Sappino, C., Primitivo, L., De Angelis, M., Domenici, M. O., Mastrodonato, A., Romdan, I. B., Tatangelo, C., Suber, L., Pilloni, L., Ricelli, A., and Righi, G.
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Nitroaldol reaction ,General Chemical Engineering ,Enantioselective synthesis ,Nanoparticle ,Alcohol ,General Chemistry ,Diethylzinc ,Combinatorial chemistry ,Article ,Catalysis ,Chemistry ,chemistry.chemical_compound ,chemistry ,Magnetic nanoparticles ,asymmetric catalysis ,magnetic nanoparticles ,Henry reaction ,QD1-999 ,Superparamagnetism - Abstract
With the aim to easily recover and reuse the catalyst, an efficient amino alcohol catalyst previously tested in the asymmetric addition of diethylzinc to several aromatic aldehydes has been immobilized on proper functionalized superparamagnetic core-shell magnetite-silica nanoparticles and employed in the Henry reaction in the semi-homogeneous phase. The nanocatalyst exhibits a promising catalytic activity that remains unchanged in the three catalytic cycles performed. The results prove that highly efficient catalysts, by being immobilized on suitable magnetic nanosupports, can be easily recovered and reused, maintaining their catalytic behavior.
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- 2019
13. Design of a fluorescent and clickable Ag
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Gaetano, Campi, Lorenza, Suber, Giuliana, Righi, Ludovica, Primitivo, Martina, De Angelis, Daniela, Caschera, Luciano, Pilloni, Alessandra, Del Giudice, Amedeo, Palma, Mauro, Satta, Alessandro, Fortunelli, and Luca, Sementa
- Abstract
Fluorescent atomically precise Ag
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- 2021
14. Stereodivergent synthesis of piperidine iminosugars 1-deoxy-D-nojirimycin and 1-deoxy-D-altronojirimycin
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Alessandra Ricelli, Martina De Angelis, Maria Grazia De Dominicis, Marianna D’Alessio, Paolo Mencarelli, Carla Sappino, Giuliana Righi, Sara Sannino, Emanuela Mandic, and Ludovica Primitivo
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010405 organic chemistry ,Chemistry ,Stereochemistry ,Organic Chemistry ,Epoxide ,Stereodivergent synthesis ,Optically active ,010402 general chemistry ,Ring (chemistry) ,01 natural sciences ,Biochemistry ,Iminosugars ,0104 chemical sciences ,1-deoxy-D-nojirimycin ,Nojirimycin ,chemistry.chemical_compound ,Stereospecificity ,Dihydroxylation ,1-deoxy-D-altronojirimycin ,Asymmetric dihydroxylation ,Drug Discovery ,Piperidine ,Azide - Abstract
A stereodivergent approach for producing piperidine iminosugars has been developed employing a common optically active precursor. The key steps of the synthetic pathway are the double diastereoselection in the asymmetric dihydroxylation of the suitable chiral vinyl epoxy ester and the regio- and stereospecific opening of the epoxide ring with azide. The synthesis of two piperidine iminosugars, 1-deoxy-D-altronojirimycin and 1-deoxy-D-nojirimycin, was achieved by two related routes.
- Published
- 2021
15. Fine-tuning synthesis of fluorescent silver thiolate nanoclusters
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Kshitij Khanna, Luciano Pilloni, Martina De Angelis, Ludovica Primitivo, Daniela Caschera, Lorenza Suber, and Giuliana Righi
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Thermogravimetric analysis ,Materials science ,Infrared ,Biomedical Engineering ,atomic precise silver nanoparticle ,Bioengineering ,02 engineering and technology ,engineering.material ,010402 general chemistry ,Photochemistry ,01 natural sciences ,Fluorescence spectroscopy ,Nanoclusters ,Nanomaterials ,Scanning transmission electron microscopy ,General Materials Science ,synthesis silver thiolate nanocluster ,fluorescent silver thiolate nanocluster ,monolayer protected silver nanoparticle ,General Chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Fluorescence ,0104 chemical sciences ,engineering ,Noble metal ,0210 nano-technology - Abstract
Noble metal thiolate nanoclusters are a new class of nanomaterials with molecular-like properties such as high dispersibility and fluorescence in the visible and infrared spectral region, properties highly requested in biomedicine for imaging, sensing and drug delivery applications. We report on three new silver phenylethane thiolate nanoclusters, differing for slight modifications of the preparation, i.e., the reaction solvent and the thiolate quantity, producing changes in the nanocluster composition as well as in the fluorescence behavior. All samples, excited in the range 250–500 nm, emit around 400 and 700 nm differing in the emission maxima and behavior. The silver thiolate nanoclusters have been characterized by way of C, H, S elemental analyses and Thermal Gravimetric Analysis (TGA) to determine the nanocluster composition, Scanning Transmission Electron Microscopy (STEM) to investigate the nanocluster morphology and UV-Vis and fluorescence spectroscopy to study their optical properties.
- Published
- 2021
16. Stereocontrolled total synthesis of iminosugar 1,4-dideoxy-1,4-imino-D-iditol
- Author
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Ludovica Primitivo, Martina De Angelis, Giuliana Righi, Sonia Agostinelli, Claudia Lucarini, Alessandra Ricelli, and Carla Sappino
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Stereochemistry ,4-imino-D-galactitolAsymmetric dihydroxylation ,Iminosugars1 ,Iminosugar ,Cinchona Alkaloids ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,chemistry.chemical_compound ,4-Dideoxy-1 ,Iditol ,iminosugars ,1,4-Dideoxy-1,4-imino-D-galactitol ,1,4-Dideoxy-1,4-imino-D-iditol ,Asymmetric dihydroxylation ,Iminosugars ,Olefin fiber ,Molecular Structure ,010405 organic chemistry ,Chemistry ,Ligand ,Organic Chemistry ,4-imino-D-iditol1 ,Total synthesis ,Stereoisomerism ,General Medicine ,Optically active ,0104 chemical sciences ,Imino Sugars ,Dihydroxylation - Abstract
The first stereocontrolled total synthesis of iminosugar 1,4-dideoxy-1,4-imino-D-iditol is described. The key step in our approach was the double diastereoselection in the asymmetric dihydroxylation (AD) of suitable optically active olefin, the chiral vinyl azido alcohol 9. Performing the AD using the most common Cinchona alkaloids as ligands enabled us to identify the ligand of choice for the stereodivergent synthesis of 1,4-dideoxy-1,4-imino-D-iditol and 1,4-dideoxy-1,4-imino-D-galactitol. These type of iminosugars, both natural and unnatural, are intensively studied for their promising chemotherapeutic properties against viral infections, diabetes, cancer, and tuberculosis.
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- 2020
17. Antioxidant and biological activities of hydroxytyrosol and homovanillic alcohol obtained from olive mill wastewaters of extra-virgin olive oil production
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Luciano Saso, Fabio Gionfra, Ludovica Primitivo, Martina De Angelis, Veronica Bonfantini, Sandra Incerpi, Zulema A. Percario, Jens Z. Pedersen, Simonetta Maria Bullitta, Roberto Antonioletti, Alessandra Ricelli, Ricelli, A., Gionfra, F., Percario, Z., De Angelis, M., Primitivo, L., Bonfantini, V., Antonioletti, R., Bullitta, S. M., Saso, L., Incerpi, S., and Pedersen, J. Z.
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Antioxidant ,antioxidant ,medicine.medical_treatment ,Metabolite ,Wastewater ,Chemical synthesis ,Scavenger ,Antioxidants ,Cell Line ,olive mill wastewaters ,chemistry.chemical_compound ,Olea ,medicine ,antioxidant, homovanillic alcohol, hydroxytyrosol, olive mill wastewaters, sustainable, tyrosol ,Animals ,Humans ,Food science ,Cytotoxicity ,Olive Oil ,Cell Proliferation ,Waste Products ,homovanillic alcohol ,hydroxytyrosol ,sustainable ,tyrosol ,Chemistry ,Plant Extracts ,Homovanillic Acid ,General Chemistry ,Phenylethyl Alcohol ,Rats ,Tyrosol ,Cell culture ,Hydroxytyrosol ,General Agricultural and Biological Sciences ,Reactive Oxygen Species - Abstract
Some constituents of the Mediterranean diet, such as extra-virgin olive oil (EVOO) contain substances such as hydroxytyrosol (HT) and its metabolite homovanillic alcohol (HA). HT has aroused much interest due to its antioxidant activity as a radical scavenger, whereas only a few studies have been made on the HA molecule. Both chemical synthesis and extraction techniques have been developed to obtain these molecules, with each method having its advantages and drawbacks. In this study, we report the use of tyrosol from olive mill wastewaters as a starting molecule to synthesize HT and HA, using a sustainable procedure characterized by high efficiency and low cost. The effects of HT and HA were evaluated on two cell lines, THP-1 human leukemic monocytes and L-6 myoblasts from rat skeletal muscle, after treating the cells with a radical generator. Both HT and HA efficiently inhibited ROS production. In particular, HT inhibited the proliferation of the THP-1 leukemic monocytes, while HA protected L-6 myoblasts from cytotoxicity.
- Published
- 2020
18. Linear β-amino alcohol catalyst anchored on functionalized magnetite nanoparticles for enantioselective addition of dialkylzinc to aromatic aldehydes
- Author
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Ludovica Primitivo, Alessandra Ricelli, Federica Di Pietro, Stefano Vecchio Ciprioti, Luciano Pilloni, Martina De Angelis, Lorenza Suber, Carla Sappino, Marika Iannoni, Francesco Righi, Giuliana Righi, Sappino, C., Primitivo, L., De Angelis, M., Righi, F., Di Pietro, F., Iannoni, M., Pilloni, L., Ciprioti, S. V., Suber, L., Ricelli, A., and Righi, G.
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asymmetric catalysis ,?-amino alcohols ,nucleophilic addition ,magnetite nanoparticles ,core-shell nanoparticles ,Ligand ,General Chemical Engineering ,Enantioselective synthesis ,Nanoparticle ,Alcohol ,General Chemistry ,linear b-amino alcohol catalyst ,addition of dialkylzinc to aldehydes ,Combinatorial chemistry ,Catalysis ,Magnetite Nanoparticles ,chemistry.chemical_compound ,chemistry ,Efficient catalyst ,Superparamagnetism - Abstract
A linear β-amino alcohol ligand, previously found to be a very efficient catalyst for enantioselective addition of dialkylzinc to aromatic aldehydes, has been anchored on differently functionalized superparamagnetic core-shell magnetite-silica nanoparticles (1a and 1b). Its catalytic activity in the addition of dialkylzinc to aldehydes has been evaluated, leading to promising results, especially in the case of 1b for which the recovery by simple magnetic decantation and reuse was successfully verified. This journal is
- Published
- 2020
19. Total stereocontrolled synthesis of a novel pyrrolizidine iminosugar
- Author
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Martina De Angelis, Federica Lizzio, Andrea D'Annibale, Sonia Agostinelli, Alessandra Ricelli, Roberto Antonioletti, Giuliana Righi, Ilaria Ben Rondam, Ludovica Primitivo, Luciano Bonanni, and Carla Sappino
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Asymmetric dihydroxylation ,Azidolysis ,Iminosugars ,Pyrrolizidine iminosugars ,Stereocontrolled synthesis ,Organic Chemistry ,Iminosugar ,Epoxide ,Stereoisomerism ,General Medicine ,Biochemistry ,Combinatorial chemistry ,Pyrrolidine ,Analytical Chemistry ,chemistry.chemical_compound ,chemistry ,Dihydroxylation ,Biological property ,Pyrrolizidine ,Epoxy Compounds ,Stereoselectivity ,Pyrrolizidine Alkaloids - Abstract
Herein we describe a versatile approach to the pyrrolizidine alkaloids skeleton by tailoring our original strategy already used for the pyrrolidine iminosugars synthesis. The key steps are the regio- and stereoselective azidolysis of the suitable chiral vinyl epoxide and then asymmetric dihydroxylation of the corresponding azido alcohol by using (DHQ)2AQN as the ligand. Further optimized elaborations addressed to the closure of the two rings allowed us to achieve the target iminosugar with complete stereocontrol. The wide range of pyrrolizidine iminosugars’ biological properties make them a key focus of new drug research and therefore the development of synthetic strategies for obtaining them is of decisive importance.
- Published
- 2022
20. Role of Some Food-Grade Synthesized Flavonoids on the Control of Ochratoxin A in Aspergillus carbonarius
- Author
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Martina De Angelis, Roberto Antonioletti, Alessandra Ricelli, Ludovica Primitivo, Carla Sappino, and Giuliana Righi
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Ochratoxin A ,Pharmaceutical Science ,01 natural sciences ,Flavones ,Aspergillus carbonarius ,Article ,Analytical Chemistry ,lcsh:QD241-441 ,03 medical and health sciences ,Lipoxygenase ,chemistry.chemical_compound ,flavones ,Biosynthesis ,lcsh:Organic chemistry ,mycotoxins ,Drug Discovery ,ochratoxins ,Food science ,Physical and Theoretical Chemistry ,Mycotoxin ,aspergillus carbonarius ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,biology ,aromatic hydroxylation ,010405 organic chemistry ,aspergillus ,Organic Chemistry ,Food grade ,food and beverages ,enzyme activation ,lipoxygenase ,0104 chemical sciences ,ochratoxin A ,biosynthetic pathways ,chemistry ,Chemistry (miscellaneous) ,Polyphenol ,flavonoids ,biology.protein ,Molecular Medicine - Abstract
Ochratoxin A (OTA) is a mycotoxin with a serious impact on human health. In Mediterranean countries, the black Aspergilli group, in particular Aspergillus carbonarius, causes the highest OTA contamination. Here we describe the synthesis of three polyphenolic flavonoids: 5-hydroxy-6,7-dimethoxy-flavone (MOS), 5,6-dihydroxy-7-methoxy-flavone (NEG), and 5,6 dihydroxy-flavone (DHF), as well as their effect on the prevention of OTA biosynthesis and lipoxygenase (LOX) activity in A. carbonarius cultured in a conducive liquid medium. The best control effect on OTA biosynthesis was achieved using NEG and DHF. In fungal cultures treated with these compounds at 5, 25, and 50 &mu, g/mL, OTA biosynthesis significantly decreased throughout the 8-day experiment. NEG and DHF appear to have an inhibiting effect also on the activity of LOX, whereas MOS, which did not significantly inhibit OTA production, had no effect on LOX activity. The presence of free hydroxyls in catecholic position in the molecule appears to be a determining factor for significantly inhibiting OTA biosynthesis. However, the presence of a methoxy group in C-7 in NEG could slightly lower the molecule&rsquo, s reactivity increasing OTA inhibition by this molecule at 5 &mu, g/mL. Polyphenolic flavonoids present in edible plants may be easily synthesized and used to control OTA biosynthesis.
- Published
- 2019
21. Validity and reliability of Italian version of the Non-Communicating Children's Pain Checklist: revised version
- Author
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Massimiliano Murgia, Martina De Angelis, Andrea Torquati, Valter Santilli, Alessandra Bettinelli, Chiara Di Maggio, Vincenzo Leuzzi, Rosanna Izzo, Massimiliano Mangone, Marco Paoloni, and Andrea Bernetti
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Male ,030506 rehabilitation ,medicine.medical_specialty ,validity ,Adolescent ,Population ,Validity ,Pain ,Physical Therapy, Sports Therapy and Rehabilitation ,Sensitivity and Specificity ,rehabilitation ,03 medical and health sciences ,0302 clinical medicine ,Cronbach's alpha ,Pain assessment ,Rating scale ,Intellectual disability ,medicine ,Humans ,Cognitive Dysfunction ,Internal validity ,Prospective Studies ,education ,Child ,Language ,Pain Measurement ,education.field_of_study ,reliability ,Receiver operating characteristic ,business.industry ,Reproducibility of Results ,pain ,medicine.disease ,Checklist ,Italy ,Child, Preschool ,Physical therapy ,Female ,0305 other medical science ,business ,030217 neurology & neurosurgery - Abstract
BACKGROUND The Non-Communicating Children Pain Check List-Revised (NCCPC-R) is a clinical assessment tool used to assess and measure pain in children aged 3 to 18 years, with mental and intellectual disabilities, incapable to speak. AIM Aim of our study was to test the validity and reliability of the Italian version of the NCCPC-R in children with cognitive impairment, in order to obtain a valid tool for pain assessment in these children. DESIGN Prospective observational study. SETTING Pediatric Outpatient of Physical and Rehabilitative Medicine Department, and Pediatrics, Child Neurology and Psychiatry Department, Sapienza University, Rome. POPULATION 55 non-communicating children, with severe intellectual disability, aged 3-18 years. METHODS The guidelines for "translation, adaptation, and validation of instruments or scales for cross-cultural healthcare research" were used to translate the scale, which was administered by the parents/caregivers twice for 2 consecutive days, in association with NRS (Numerical Rating Scale). The reliability of the scale was evaluated using the intra and inter-class correlation coefficient (ICCs); Cronbach alpha coefficient was used to test the internal validity of the scale; "Receiver Operating Characteristic" (ROC) curves were used to compare pain-free scores with pain scores, determining threshold scores; Pearson correlation between NCCPC-R and NRS values was measured. RESULTS The InterCC between the first and the second interviewer at T0 was 0,97, the IntraCC of the first interviewer at T0-T1 was 0,89, showing a high correlation; the Cronbach alpha coefficient at T0 was 0,97, showing a high scale's validity. Pearson correlation between NRS and NCCPC-R values at T0 was 0,54 showing a medium level of agreement (P
- Published
- 2018
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