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3 results on '"Martha A. Fernández-Galindo"'

1. Elevada frecuencia de metilación del promotor de MLH1 mediada por sexo y edad en tumores colorrectales de pacientes mexicanos

Author

José M. Moreno-Ortiz, Josselyn Jiménez-García, Melva Gutiérrez-Angulo, Ma. de la Luz Ayala-Madrigal, Anahí González-Mercado, Christian O. González-Villaseñor, Beatriz A. Flores-López, Carlos Alvizo-Rodríguez, Jesús A. Hernández-Sandoval, Martha A. Fernández-Galindo, Víctor Maciel-Gutiérrez, Helen Ramírez-Plascencia, and Ruth Ramírez-Ramírez

Subjects
Public aspects of medicine, RA1-1270, Internal medicine, RC31-1245
Published
2021
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2. Molecular Profiling of Tumor Tissue in Mexican Patients with Colorectal Cancer

Author

Beatriz Armida Flores-López, María de la Luz Ayala-Madrigal, José Miguel Moreno-Ortiz, Jorge Peregrina-Sandoval, Miguel Ángel Trujillo-Rojas, José Luis Venegas-Rodríguez, Rosario Hernández-Ramírez, Martha Alejandra Fernández-Galindo, and Melva Gutiérrez-Angulo

Subjects
colorectal cancer, massive parallel sequencing, pathogenic variant, likely pathogenic variant, somatic variants, exome sequencing, Biology (General), QH301-705.5
Abstract

Colorectal cancer is a heterogeneous disease with multiple genomic changes that influence the clinical management of patients; thus, the search for new molecular targets remains necessary. The aim of this study was to identify genetic variants in tumor tissues from Mexican patients with colorectal cancer, using massive parallel sequencing. A total of 4813 genes were analyzed in tumoral DNA from colorectal cancer patients, using the TruSight One Sequencing panel. From these, 192 variants with clinical associations were found distributed in 168 different genes, of which 46 variants had not been previous reported in the literature or databases, although genes harboring those variants had already been described in colorectal cancer. Enrichment analysis of the affected genes was performed using Reactome software; pathway over-representation showed significance for disease, signal transduction, and immune system subsets in all patients, while exclusive subsets such as DNA repair, autophagy, and RNA metabolism were also found. Those characteristics, whether individual or shared, could give tumors specific capabilities for survival, aggressiveness, or response to treatment. Our results can be useful for future investigations targeting specific characteristics of tumors in colorectal cancer patients. The identification of exclusive or common pathways in colorectal cancer patients could be important for better diagnosis and personalized cancer treatment.

Published
2022
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3. High frequency of MLH1 promoter methylation mediated by gender and age in colorectal tumors from Mexican patients

Author

José M. Moreno-Ortiz, Josselyn Jiménez-García, Melva Gutiérrez-Angulo, Ma. de la Luz Ayala-Madrigal, Anahí González-Mercado, Christian O. González-Villaseñor, Beatriz A. Flores-López, Carlos Alvizo-Rodríguez, Jesús A. Hernández-Sandoval, Martha A. Fernández-Galindo, Víctor Maciel-Gutiérrez, Helen Ramírez-Plascencia, and Ruth Ramírez-Ramírez

Subjects
Male, Sex Factors, Age Factors, Humans, CpG Islands, Female, General Medicine, DNA Methylation, Middle Aged, Colorectal Neoplasms, MutL Protein Homolog 1, Promoter Regions, Genetic, Mexico
Abstract

Several genes determine the development of colorectal cancer (CRC), such as MLH1, which encodes a protein that participates in DNA repair. MLH1 hypermethylation has been associated with gene silencing.To analyze the methylation of five regions of MLH1 CpG island in colorectal tumors from Mexican patients.One hundred and one tumor tissue samples were obtained from Mexican patients with CRC who provided informed consent. DNA was subjected to bisulfite conversion. Methylation of all five regions of the CpG island was evaluated using methylation-specific PCR.The frequency of methylation in Mexican patients with CRC was 25%. Regions A and B methylation was the main observed pattern (60%). Female patients showed a higher frequency of methylation (71%; OR 3.085; CI: 1.85-8.03; p = 0.02), and out of total methylated samples, 80% corresponded to individuals older than 45 years (p0.05).We calculated a methylation frequency for the MLH1 gene of 25% in Mexican patients with CRC, with this being the first report for this population. Female patients and patients older than 45 years showed a higher frequency of methylation.Varios genes determinan el desarrollo de cáncer colorrectal (CCR), como MLH1, el cual codifica una proteína que participa en la reparación del ADN. La hipermetilación de MLH1 ha sido asociado con silenciamiento génico.Analizar la metilación de cinco regiones de la isla CpG de MLH1 en tumores colorrectales de pacientes mexicanos.Se obtuvieron 101 muestras de tejido tumoral de pacientes mexicanos con CCR, quienes proporcionaron su consentimiento informado. El ADN fue sometido a conversión por bisulfito. La metilación de las cinco regiones de la isla CpG fue evaluada utilizando PCR específica para metilación.La frecuencia de metilación en pacientes mexicanos con CCR fue del 25%. La metilación de las regiones A y B fue el principal patrón observado (60%). Las pacientes de sexo femenino mostraron una mayor frecuencia de metilación (71%) (odds ratio: 3.085; intervalo de confianza; 1.85-8.03; p = 0.02); y del total de muestras metiladas, el 80% fueron individuos mayores de 45 años (p0.05).Calculamos una frecuencia de metilación para el gen MLH1 del 25% en pacientes mexicanos con CCR, siendo el primer reporte para esta población. Pacientes de sexo femenino y pacientes mayores de 45 años mostraron una mayor frecuencia de metilación.

Published
2022

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