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Salmonella Enteritidis is the main serotype responsible for human salmonellosis in the European Union. One of the main sources of Salmonella spp. in the food chain are poultry products, such as eggs or chicken meat. In recent years, molecular methods have become an alternative to culture dependent methods for the rapid screening of Salmonella spp. In this work, the strain S. Enteritidis S1400, and previously isolated and characterized bacteriophage PVP-SE2, were used to develop and evaluate a same-day detection method combining Phage Amplification and Loop-mediated isothermal amplification (PA-LAMP) to specifically detect viable S. Enteritidis in chicken breast. This method is based on the detection of the phage DNA rather than bacterial DNA. The virus is added to the sample during pre-enrichment in buffered peptone water, where it replicates in the presence of viable S. Enteritidis. The detection of phage DNA allows, on the one hand to detect viable bacteria, since viruses only replicate in them, and on the other hand to increase the sensitivity of the method since for each infected S. Enteritidis cell, hundreds of new viruses are produced. Two different PA-LAMP detection strategies were evaluated, a real time fluorescence and a naked-eye detection. The present method could down to 0.2 fg/L of pure phage DNA and a concentration of viral particles of 2.2 log PFU/mL. After a short Salmonella recovery step of 3 h and a co-culture of 4 h of the samples with phage particles, both real-time fluorescence and naked-eye method showed a LoD95 of 6.6 CFU/25 g and a LoD50 of 1.5/25 g in spiked chicken breast samples. The entire detection process, including DNA extraction and LAMP analysis, can be completed in around 8 h. In the current proof-of-concept, the novel PA-LAMP obtained comparable results to those of the reference method ISO 6579, to detect Salmonella Enteritidis in poultry meat., Dr. Alexandre Lamas was funded by a postdoctoral fellowship from Xunta de Galicia (Axudas de apoio a étapa de formación posdoutoral IN606B (Modalidade A)). Dr. Alejandro Garrido-Maestu acknowledges funding from the Fundação para a Ciência e Tecnologia through the Scientific Employment Stimulus Program (2021.02810.CEECIND)., info:eu-repo/semantics/publishedVersion

Background: Ready-to-eat products, such as leafy greens, must be carefully controlled as they are directly consumed without any treatment to reduce the presence of potential pathogens. Food industries, especially those that process products with short shelf-life, demand rapid detection of foodborne pathogens such as Shiga Toxinproducing Escherichia coli (STEC). In this sense, molecular methods can fulfill both requirements of turnaround time and consumer safety. The most popular rapid methods are those based on real-time PCR (qPCR) however, vegetables contain inhibitory compounds that may inhibit the amplification reaction thus, there is a need for novel sample preparation protocols. Results: In the current study, a low-cost sample treatment based on sequential filtration steps was developed. This protocol was combined with covalent organic frameworks (COFs), and compared against a chelating resin, to evaluate their performance by multiplex qPCR targeting the major virulence genes of STEC, namely stx1, stx2, and eae, along with the rfbE for the specific identification of serogroup O157 due to its particularly high incidence, and an Internal Amplification Control to assess reaction inhibition. The optimized sample treatment effectively removed vegetable qPCR inhibitory compounds, and it was possible to detect STEC in spiked ready-toeat salad samples in one working day, roughly 5 h, with an LOD50 of 8.7 CFU/25 g with high diagnostic sensitivity and specificity. The method was also assessed in samples with cold-stressed bacteria with good results, further demonstrating its applicability. Significance: It was demonstrated for the first time that COFs are suitable for DNA extraction and purification. In addition to this, due to the tunable nature of these materials, it is envisioned that future modifications in terms of pore size or combination with magnetic materials, will allow to further improve their performance. In addition to this, the rapid and low-cost sample treatment protocol developed demonstrated suitable for the rapid screening of STEC vegetable samples. published

Nucleic acid amplification-based techniques have gained acceptance by the scientific, and general, community as reference methodologies for many different applications. Since the development of the gold standard of these techniques, polymerase chain reaction (PCR), back in the 1980s many improvements have been made, and alternative techniques emerged reporting improvements over PCR. Among these, isothermal amplification approaches resulted of particular interest as could overcome the need of specialized equipment to accurately control temperature changes, but it was after year 2000 that these techniques have flourished in a huge number of novel alternatives with many different degrees of complexities and requirements. An added value is their possibility to be combined with many different naked-eye detection strategies, simplifying the resources needed, allowing to reduce cost, and serving as the basis for novel developments of lab-on-chip systems, and miniaturized devices, for point-of-care testing. In this review, we will go over different types of naked-eye detection strategies, combined with isothermal amplification. This will provide the readers up-to-date information for them to select the most appropriate strategies depending on the particular needs and resources for their experimental setup.

Introdução: Anatomia é a ciência que estuda a estrutura do corpo humano e a integração dos sistemas orgânicos, sendo então necessário um conhecimento anatômico detalhado para atuar de forma clínica e cirúrgica. Entretanto, na medicina atual, há uma redução da carga horária dedicada à anatomia, bem como a mudança de metodologia de ensino, o que ocasionou em um déficit no ensino e aprendizado da anatomia nas instituições de ensino. Objetivo: Avaliar o ensino e aprendizagem da anatomia no curso de medicina. Metodologia: Revisão bibliográfica descritiva em caráter exploratório de abordagem qualitativa, a partir de publicações científicas encontradas nas bases de dados da PUBMED, SCIELO e Biblioteca Virtual em Saúde. Resultados: O estudo contou com a amostra de 8 artigos, dos quais a necessidade de estudo a partir de cadáver foi citada em todos os artigos, a capacitação de profissionais foi explanada em 5 trabalhos, sobre metodologias de estudos nas instituições superiores foram citados em 5 revisões científicas. Discussão: É observado que a anatomia nas instituições de medicina tem tido mudanças ao longo do tempo, com a diminuição da carga horária, informatização do conteúdo e introdução tecnológica. Porém, é evidente que para estudar e compreender outras disciplinas do currículo acadêmico, é necessário um profundo conhecimento anatômico. Conclusão: É necessário que haja integração das matérias para que tenha um melhor raciocínio clínico, o que não é uma realidade nas maiorias das faculdades, uma vez que são abordadas separadamente entre os semestres. Além da falta de cadáver e profissionais capacitados em algumas instituições. Introduction: Anatomy is the science that studies the structure of the human body and the integration of organic systems, requiring detailed anatomical knowledge to act in a clinical and surgical manner. However, in current medicine, there is a reduction in the workload dedicated to anatomy, as well as a change in teaching methodology, which caused a deficit in the teaching and learning of anatomy in educational institutions. Objective: To evaluate the teaching and learning of anatomy in the medical course. Methodology: Descriptive bibliographic review in an exploratory qualitative approach, based on scientific publications found in the PUBMED, SCIELO and Virtual Health Library databases. Results: The study had a sample of 8 articles, of which the need for study from cadavers was mentioned in all articles, the training of professionals was explained in 5 works, on methodologies of studies in higher institutions were mentioned in 5 scientific reviews. Discussion: It is observed that the anatomy in medical institutions has changed over time, with the reduction of workload, computerization of content and technological introduction. However, it is evident that in order to study and understand other disciplines in the academic curriculum, a deep anatomical knowledge is necessary. Conclusion: It is necessary to integrate the subjects so that there is a better clinical reasoning, which is not a reality in most faculties, since they are addressed separately between the semesters. In addition to the lack of corpses and trained professionals in some institutions. Introducción: La anatomía es la ciencia que estudia la estructura del cuerpo humano y la integración de los sistemas orgánicos, requiriendo conocimientos anatómicos detallados para actuar de manera clínica y quirúrgica. Sin embargo, en la medicina actual existe una reducción de la carga horaria dedicada a la anatomía, así como un cambio en la metodología de enseñanza, lo que provocó un déficit en la enseñanza y aprendizaje de la anatomía en las instituciones educativas. Objetivo: Evaluar la enseñanza y el aprendizaje de la anatomía en la carrera de medicina. Metodología: Revisión bibliográfica descriptiva con enfoque cualitativo exploratorio, a partir de publicaciones científicas encontradas en las bases de datos PUBMED, SCIELO y Biblioteca Virtual en Salud. Resultados: El estudio tuvo una muestra de 8 artículos, de los cuales se mencionó en todos los artículos la necesidad de estudio a partir de cadáveres, en 5 trabajos se explicó la formación de profesionales, en 5 revisiones científicas se mencionó sobre metodologías de estudios en instituciones superiores. Discusión: Se observa que la anatomía en las instituciones médicas ha cambiado con el tiempo, con la reducción de la carga de trabajo, informatización de contenidos e introducción tecnológica. Sin embargo, es evidente que para estudiar y comprender otras disciplinas del currículo académico es necesario un profundo conocimiento anatómico. Conclusión: Es necesario integrar las asignaturas para que exista un mejor razonamiento clínico, lo cual no es una realidad en la mayoría de las facultades, ya que se abordan por separado entre los semestres. Además de la falta de cadáveres y profesionales capacitados en algunas instituciones.

This study presents the development of a lab-on-a-chip (LoC) by integrating a graphene field-effect transistor (FET) chip with a programmable microfluidic device for DNA detection. The real-time biochemical events on the graphene FET chip were monitored through Dirac voltage shift data from the portable graphene curve reader with changes dependent on the fluidic flow into the sensing interface by a fully automated programmable microfluidic system. High sensitivity with high reliability can be obtained with a nine-graphene sensor layout on a single chip. The portable graphene curve reader also provides a tunable electrical parameter setup and straightforward data acquisition. Fluidic control was performed through a multi-position valve, allowing sequential commands for liquid injection into the polydimethylsiloxane (PDMS) flow cell mounted on the sensing chip. The flow cell design with impinging jet geometry and the microfluidic system packaging offer high precision and portability as a less laborious and low-cost sensing setup. The merged system allows for various functionalities, including probe DNA (pDNA) immobilization, a blocking step, and DNA hybridization with stable signal output autonomously, even in a long-run experimental setup. As a DNA sensor, the proposed prototype has demonstrated a high sensitivity of ~44 mV/decade of target DNA concentration, with an outstanding limit of detection (LoD) of ~0.642 aM, making it one of the most sensitive sensors reported up to date. The programmable device has demonstrated essential versatilities for biomolecular detection in a fully portable and automated platform., This research is supported by PORTGRAPHE-Control of Port and Douro Wines authenticity using graphene DNA sensors project co-funded by Fundação para a Ciência e a Tecnologia (FCT) Portugal (PTDC/BIA-MOL/31069/2017) and the ERDF through COMPETE2020 (POCI-01–0145-FEDER-031069). One of the authors (Telma Domingues) acknowledges a Ph.D. grant from Fundação para a Ciência e a Tecnologia (FCT) Portugal (SFRH/BD/08181/2020). FCT partially supported University of Minho´s research in the Strategic Funding UIDB/04650/2020.

6 pages, 4 tables, 1 figure.-- Under a Creative Commons license, Listeria monocytogenes is a major foodborne pathogen which mainly infects susceptible individuals through the consumption of contaminated foods. To this end, ready-to-eat (RTE) food products are of particular concern as this microorganism is widely distributed, can survive, and even grow, under adverse conditions, and thus must be carefully controlled. In the present study, an interlaboratory ring trial was organized to evaluate an open formula qPCR-based method for the detection of L. monocytogenes. The molecular method was evaluated on a novel RTE seafood product, developed in the framework of a European project, the SEAFOODAGE (EAPA_758/2018). Six laboratories located in Spain and Portugal participated in the study, and the results obtained indicated that this new method presented high diagnostic sensitivity (100%) reaching a low limit of detection (, This work was financially supported by the Seafood Age project, which was co-financed by the Interreg Atlantic Area Program (EAPA_758/2018) though the European Development Fund (ERDF). Mrs. Sarah Azinheiro was financed by a Ph.D. grant from the Fundação para a Ciência e a Tecnologia (SFRH/BD/140396/2018). Dr. Alejandro Garrido-Maestu and Luís D. R. Melo acknowledge funding from the Fundação para a Ciência e Tecnologia through the Scientific Employment Stimulus Program (2021.02810. CEECIND and 2021.00221. CEECIND, respectively). This study was supported by the Fundação para a Ciência e a Tecnologia (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit

Plasmonic nanosensors for label-free detection of DNA require excellent sensing resolution, which is crucial when monitoring short DNA sequences, as these induce tiny peak shifts, compared to large biomolecules. We report a versatile and simple strategy for plasmonic sensor signal enhancement by assembling multiple (four) plasmonic sensors in series. This approach provided a fourfold signal enhancement, increased signal-to-noise ratio, and improved sensitivity for DNA detection. The response of multiple sensors based on AuNSpheres was also compared with AuNRods, the latter showing better sensing resolution. The amplification system based on AuNR was integrated into a microfluidic sequential injection platform and applied to the monitoring of DNA, specifically from environmental invasive species—zebra mussels. DNA from zebra mussels was log concentration-dependent from 1 to 1 × 106 pM, reaching a detection limit of 2.0 pM. In situ tests were also successfully applied to real samples, within less than 45 min, using DNA extracted from zebra mussel meat. The plasmonic nanosensors’ signal can be used as a binary output (yes/no) to assess the presence of those invasive species. Even though these genosensors were applied to the monitoring of DNA in environmental samples, they potentially offer advantage in a wide range of fields, such as disease diagnostics. info:eu-repo/semantics/acceptedVersion

Foodborne pathogens are still a significant source of morbidity and mortality worldwide. In addition to this the current methodologies to track these microorganisms cannot cope with the current intensive production systems, thus novel methods are of outmost importance. DNA-based methods have already demonstrated suitable to address this issue, but most of them are targeted methods such as real-time PCR (qPCR), meaning that one will only find what is looking for, thus taking the risk of missing relevant pathogens in a given sample. To overcome this limitation we have developed an easy-to-implement methodology which enables the detection of several pathogens simultaneously by using long-read Next Generation Sequencing (NGS) with MinION. The method was named “semi-targeted” due to the combination of a non-targeted detection method, NGS, with the usage of selective media in order to partially eliminate non-pathogenic interfering bacteria. To this end, we included an enrichment step for the recovery of different pathogens, namely Salmonella Enteritidis and Typhimurium, Listeria monocytogenes and Escherichia coli O157:H7, after DNA extraction and library preparation, the samples were analyzed with MinION implementing the low-cost Flongle Flow Cells. The methodology was successfully evaluated in spiked milk samples with an excellent agreement with the results obtained by qPCR and culture-based methods. The method can provide accurate results after only 2 h of sequencing. Sample multiplexing, along with the lower cost of the Flongle Flow Cells and the reduced price of the MinION platform, make the assay cost-effective that is of importance for the food industry. Starting the method with a classical microbiological approach, the enrichment, the method is easy to implement in testing laboratories, it provides flexibility in terms of potential pathogens to be detected, and the positive results can be easily confirmed following culture-based, or other type, of confirmation procedures.

Introduction: Abdominal trauma has high morbidity and mortality, and it is essential for its diagnosis would be made faster to minimize injuries. In order to achieve the quality of support protocols for the traumatized victim, programs are used, using the severity indices. Objective: To assess the profile of victims of abdominal trauma, with or whithout politrauma, using the severity indices TRISS and TRISS-like, attended in a critical axis of an urgency and emergency hospital in the state of Sergipe. Methods: Cross-sectional, prospective observational study, with a quantitative approach to victims of abdominal trauma. It was used an instrument of its own elaboration, having as a base or instrument of Domingues (2013), that provides objective questions with physiological and sociodemographic data on the topography and clinical information of the study. Results: Sample of 42 patients, obtained a mean of 33.1 years, with 81% being male and 19% female, whereas 61.9% were affected by transport with a difference between mechanisms. blunt 66.7% and penetrating mechanism 33.3%. All the accidents 100% received pre-hospital care, being that 28.6% died and 50% required ICU care. The TRISS-like group has a lower probability of survival than the TRISS (7.1% vs 97.2%). Conclusion: Due to the predominant cause of abdominal trauma, there was an accident of transport as a contusive mechanism prevailing, mostly homens, adults, with an average age of 33.1 years. All the victims receive pre-hospital support and a considerable death tax. Introducción: El traumatismo abdominal tiene una alta morbimortalidad, por lo que es fundamental que su diagnóstico se realice lo antes posible para minimizar las lesiones. Para mejorar la calidad de los protocolos de apoyo a la víctima traumatizada, se utilizan programas, utilizando los índices de gravedad. Objetivo: Evaluar el perfil de víctimas de traumatismo abdominal, con o sin politraumatismo, utilizando los índices de gravedad TRISS y TRISS-like, atendidos en el eje crítico de un hospital de referencia en urgencia y emergencia en el estado de Sergipe. Métodos: Estudio transversal, observacional, prospectivo, con abordaje cuantitativo a víctimas de traumatismo abdominal. Se utilizó un instrumento de elaboración propia, basado en el instrumento Domingues (2013), el cual tiene preguntas objetivas con datos fisiológicos y sociodemográficos sobre la topografía e información clínica del estudio. Resultados: De la muestra de 42 pacientes se obtuvo una edad promedio de 33,1 años, siendo 81% varones y 19% mujeres, de los cuales 61,9% sufrió un accidente de transporte con diferencia entre mecanismo romo 66,7% y mecanismo penetrante 33,3%. Todos los accidentes tuvieron un 100% de atención prehospitalaria, un 28,6% murieron y un 50% necesitaron atención en UCI. El grupo similar a TRISS tuvo una menor probabilidad de supervivencia que el grupo TRISS (7,1% frente a 97,2%). Conclusión: La causa predominante de traumatismo abdominal fue un accidente de transporte con mecanismo contundente, mayoritariamente hombres, adultos, con una edad media de 33,1 años. Todas las víctimas tenían apoyo prehospitalario y una tasa de mortalidad considerable. Introdução: O trauma abdominal tem uma alta morbidade e mortalidade, e é fundamental que o seu diagnóstico seja feito o mais rápido possível para minimizar as lesões. Com o objetivo de melhorar a qualidade dos protocolos de suporte à vítima traumatizada, são utilizados programas, usando os índices de gravidade. Objetivo: Avaliar o perfil das vítimas de trauma abdominal, com ou sem politrauma, utilizando os índices de gravidade TRISS e TRISS-like, atendidas no eixo crítico de um hospital referência em urgência e emergência do estado de Sergipe. Métodos: Estudo transversal, observacional do tipo prospectivo, com abordagem quantitativa das vítimas de trauma abdominal. Utilizou-se um instrumento de elaboração própria, tendo como base o instrumento de Domingues (2013), que possui questões objetivas com dados fisiológicos e sociodemográficos sobre a topografia e informações clínicas do estudo. Resultados: Da amostra de 42 pacientes, obteve-se uma média de idade de 33,1 anos, com 81% sendo do sexo masculino e 19% sendo do sexo feminino, em que 61,9% sofreu acidente de transporte com diferença entre mecanismo contuso 66,7% e mecanismo penetrante 33,3%. Todos os acidentes 100% tiveram atendimento pré-hospitalar, sendo que 28,6% foram a óbito e 50% precisaram de cuidados em UTI. O grupo TRISS-like teve menor probabilidade de sobrevida que o TRISS (7,1% vs 97,2%). Conclusão: A causa predominante de trauma abdominal foi acidente de transporte com o mecanismo contuso prevalecendo, maior parte homens, adultos, idade média de 33,1 anos. Todas as vítimas tiveram suporte pré-hospitalar e a taxa de óbito considerável.

The continuous spread of invasive alien species, as zebra mussel (Dreissena polymorpha), is a major global concern and it is urgent to stop it. Early stages of an invasion are crucial and challenging; however, detection tools based on environmental DNA analysis are promising alternatives. We present an alternative DNA target amplification strategy for signal enhancement followed by dual-mode colorimetric naked eye and optical smartphone analysis for the early detection of zebra mussel environmental DNA. Target amplification was designed based on the nicking endonuclease probe cleavage upon probe and complementary target hybridization. The cleaved/intact probe interacts with DNA-modified nanoparticles for colorimetric detection. We have demonstrated that enzyme amplification strategy enhanced 12-fold the sensitivity by naked eye detection, achieving a detection limit of ~8 nM (4.48×1010 copies) in controlled conditions, whereas target in complex environmental samples allowed the detection of 22.5 nM (1.26×1011 copies). Competitive assays also showed that the system can discriminate specific zebra mussel DNA sequences from other DNA sequences. Additionally, smartphone analysis for DNA quantification further improved the sensitivity of its detection by 130-fold, more than 2 orders of magnitude, when applied to environmental samples. The limit of detection to 0.17 nM (9.52×108 copies) is based on RGB coordinates, which is especially relevant to monitor early aggregation stages, being more accurate and reducing naked eye detection subjectivity. DNA extracted from zebra mussel meat, zebra mussel contaminated river water, and non-contaminated river water samples were successfully tested. Dual-mode colorimetric detection is useful in field analysis without the need for expensive laboratory equipment.

Liquid-gated Graphene Field-Effect Transistors (GFET) are ultrasensitive bio-detection platforms carrying out the graphene&rsquo, s exceptional intrinsic functionalities. Buffer and dilution factor are prevalent strategies towards the optimum performance of the GFETs. However, beyond the Debye length (&lambda, D), the role of the graphene-electrolytes&rsquo, ionic species interactions on the DNA behavior at the nanoscale interface is complicated. We studied the characteristics of the GFETs under different ionic strength, pH, and electrolyte type, e.g., phosphate buffer (PB), and phosphate buffer saline (PBS), in an automatic portable built-in system. The electrostatic gating and charge transfer phenomena were inferred from the field-effect measurements of the Dirac point position in single-layer graphene (SLG) transistors transfer curves. Results denote that &lambda, D is not the main factor governing the effective nanoscale screening environment. We observed that the longer &lambda, D was not the determining characteristic for sensitivity increment and limit of detection (LoD) as demonstrated by different types and ionic strengths of measuring buffers. In the DNA hybridization study, our findings show the role of the additional salts present in PBS, as compared to PB, in increasing graphene electron mobility, electrostatic shielding, intermolecular forces and DNA adsorption kinetics leading to an improved sensitivity.

Lactoferrin is a multifunctional protein of the transferrin family and is known as a biomarker for various clinical diseases including urinary tract infection (UTI). However, wide concentration range of lactoferrin in urine samples due to the high interpatient variations, requires a more practical biosensor. In this article, we used a novel multivalent aptamer immobilized on the surface of screen-printed gold electrode (aptamer/SPGE) to develop the first electrochemical aptasensor for label-free detection of lactoferrin with wide dynamic detection range and high sensitivity. The performance of the fabricated biosensor was tested using electrochemical impedance spectroscopy and differential pulse voltammetry. The multivalent aptamer as the bioreceptor with high affinity and good specificity against human lactoferrin, acts to enhance the electrochemical signals and widen the working window. The aptamer/SPGE demonstrates superior sensing performances for lactoferrin in buffer solution, with a wide linear range of 10 to 1300 ng/mL with LOD of 0.9 ng/mL, as well as high selectivity, and excellent reproducibility. Besides, the constructed aptasensor was successfully applied to quantify lactoferrin concentrations in spiked urine solutions. Owing to excellent sensitivity, ease of miniaturization, simple sensing procedure, low-cost, and fast response, the proposed electrochemical aptasensor indicates a great potential towards the development of lactoferrin analysis systems, which would be helpful in the early diagnosis of UTI.

Miniaturization of DNA-based techniques can bring interesting advantages for food analysis, such as portability of complex analytical procedures. In the olive oil industry, miniaturization can be particularly interesting for authenticity and traceability applications, through in situ control of raw materials before production and/or the final products. However, variety identification is challenging, and implementation on miniaturized settings must be carefully evaluated, starting from the selected analytical approach. In this work, SSR- and SNP-based genotyping strategies were investigated for the identification and differentiation of two olive varieties from the Northwest of Spain. For the selected SNPs two genotyping methods were tested: real-time allele-specific PCR and high resolution melting analysis. These methods were compared and evaluated regarding their potential for integration in a microfluidic device. Both SNP-based methods proved to be successful for identification of the selected varieties, however real-time allele-specific PCR was the one that achieved the best results when analyzing mixtures, allowing the identification of both monovarietal samples and mixtures of the varieties tested with up to 25%.

Purification and concentration of DNA is a critical step on DNA-based analysis, which should ensure efficient DNA isolation and effective removal of contaminants that may interfere with downstream DNA amplification. Complexity of samples, minute content of target analyte, or high DNA fragmentation greatly entangles the success of this step. To overcome this issue, we designed and fabricated a novel miniaturized disposable device for a highly efficient DNA purification. The microfluidic device showed binding efficiency and elution yield of 90.1% and 86.7%, respectively. Moreover, the effect of DNA fragmentation, a parameter that has not been previously addressed, showed a great impact in the recovery step. The microfluidic system integrated micropillars with chitosan being used as the solid-phase for a pH-dependent DNA capture and release. We have showed the potential of the device in the successful purification of environmental DNA (eDNA) from river water samples contaminated with Dreissena polymorpha, an invasive alien species responsible for unquestionable economic and environmental consequences in river water basins. Additionally, the device was also able to concentrate the DNA extract from highly diluted samples, showing promising results for the early detection of such invasive species, which may allow prompt measures for a more efficient control in affected areas. Suitability for integration with downstream DNA analysis was also demonstrated through qPCR analysis of the samples purified with the microfluidic device, allowing detection of the target species even if highly diluted.