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This article belongs to the Topic Applied Sciences in Functional Foods., Iberian ham is one of the most representative Spanish products and presents an excellent nutritional and sensory quality. Iberian ham trimming fat is considered a by-product and to give a new use to this remaining part could represent a healthy and innovative option for obtaining sustainable foods. The purpose of this work was to obtain a new bioactive ingredient from Iberian ham trimming fat with the highest amount of antioxidants and monounsaturated fatty acids (MUFA), using a new non-invasive solvent-free method. To obtain the essence, two different extraction procedures were carried out. After fatty acid characterization, degree of acidity, peroxide index and a basic sensory analysis were performed. Antioxidant in vitro activity and total phenolic compounds (TPC) were also determined. This new ingredient showed a better sensory profile than raw ham fat, a lower degree of acidity, a higher content of MUFAs, and also showed a higher antioxidant capacity and an increase in phenolic compounds compared to the raw material. This bioactive essence could be used as a food, a cosmetic or a nutraceutical ingredient to prevent certain diseases related to oxidative stress and could also contribute to the maintenance of the circular economy., This research and the APC was funded by a grant from Cárnicas Joselito S.A, Guijuelo, Spain.

This research aimed to investigate the biological properties of different hydrolysates derived from industrial and laboratory defatted rice bran proteins. Industrial and laboratory defatted rice bran protein concentrates were hydrolyzed with alcalase or flavorzyme. The degree of hydrolysis (DH), oxygen radical absorbance capacity (ORAC), reducing power, total phenolic compounds (TPC), and angiotensin-converting enzyme (ACE) inhibitory activity, were determined in the hydrolysates and the molecular fractions lower than 3 kDa. Systolic blood pressure (SBP) was measured using the tail-cuff method before and after oral administration of 80 mg/kg of different rice bran protein hydrolysate (RBPH) fractions lower than 3 kDa in male spontaneously hypertensive rats (SHR) and normotensive Wistar&ndash, Kyoto (WKY) rats. The highest values of in vitro antioxidant activity and TPC were observed in RBPH with alcalase defatted by industry (RBPH2A), and, in all cases, these bioactivities were higher in the molecular fractions lower than 3 kDa. Once again, fractions lower than 3 kDa obtained with alcalase showed a potent ACE inhibitory activity (RBPH1A&lt, 3 and RBPH2A&lt, 3). The administration of RBPH1A&lt, 3 caused a significant decrease in the SBP in SHR, where the maximum decrease was reached at 8 h after administration. SBP in WKY rats was not modified after the administration of RBPH1A&lt, 3. These results suggest that the rice bran protein hydrolysates obtained from industry after treatment with alcalase could be an interesting source of bioactive peptides, with potential action on hypertension and other related pathologies.

Oxidative stress in known to contribute to the male reproductive dysfunction induced by mercury (Hg). Our study tested the hypothesis that the egg white hydrolysate (EWH), a potent antioxidant in vitro, is able to prevent the effects of prolonged Hg exposure on male reproductive system in rats. For this, rats were treated for 60 days with: a) Untreated - saline solution (i.m.); b) Hydrolysate - EWH (1 g/kg/day, gavage); c) Mercury - HgCl (1st dose 4.6 μg/kg, subsequent doses 0.07 μg/kg/day, i.m.); d) Hydrolysate-Mercury. At the end of the treatment, sperm motility, count and morphological studies were performed; Reactive Oxygen Species (ROS) levels, lipid peroxidation, antioxidant capacity, histological and immunohistochemical assays on testis and epididymis were also carried out. As results, HgCl-treatment decreased sperm number, increased sperm transit time in epididymis and impaired sperm morphology. However, these harmful effects were prevented by EWH. HgCl-treatment also increased ROS levels, lipid peroxidation and antioxidant capacity in testis and epididymis as well as promoted testicular inflammation and histological changes in epididymis. EWH improved histological and immunohistochemical alterations, probably due to its antioxidant property. In conclusion, the EWH could represent a powerful natural alternative to protect the male reproductive system against Hg-induced sperm toxicity., This research was supported by a Brazilian Government (Conselho Nacional de Desenvolvimento Científico e Tecnológico – CNPq – 203440/2014-5) and a Spanish Goverment (MINECO - AGL2012-32387; CSIC – Intramural 201570I028).

Being the metabolic syndrome a multifactorial condition, it is difficult to find adequate experimental models to study this pathology. The obese Zucker rats, which are homozygous for thefaallele, present abnormalities similar to those seen in human metabolic syndrome and are a widely extended model of insulin resistance. The usefulness of these rats as a model of non-insulin-dependent diabetes mellitus is nevertheless questionable, and they neither can be considered a clear experimental model of hypertension. Some experimental models different from the obese Zucker rats have also been used to study the metabolic syndrome. Some derive from the spontaneously hypertensive rats (SHR). In this context, the most important are the obese SHR, usually named Koletsky rats. Hyperinsulinism, associated with either normal or slightly elevated levels of blood glucose, is present in these animals, but SHR/N-corpulent rats are a more appropriated model of non-insulin-dependent diabetes mellitus. The SHR/NDmc corpulent rats, a subline of SHR/N-corpulent rats, also exhibit metabolic and histopathologic characteristics associated with human metabolic disorders. A new animal model of the metabolic syndrome, stroke-prone–SHR (SHRSP) fatty rats, was obtained by introducing a segment of the mutant leptin receptor gene from the Zucker line heterozygous for thefagene mutation into the genetic background of the SHRSP. Very recently, it has been developed as a non-obese rat model with hypertension, fatty liver and characteristics of the metabolic syndrome by transgenic overexpression of a sterol-regulatory element-binding protein in the SHR rats. The Wistar Ottawa Karlsburg W rats are also a new strain that develops a nearly complete metabolic syndrome. Moreover, a new experimental model of low-capacity runner rats has also been developed with elevated blood pressure levels together with the other hallmarks of the metabolic syndrome.

This study aims to investigate whether the egg white hydrolysate (EWH) acts on the neuropathic disorders associated with long-term Mercury (Hg) exposure in rats. 8- week-old male Wistar rats were treated for 60 days with: a) Control - saline solution (i.m.); b) Mercury - HgCl (1st dose 4.6 μg/kg, subsequent doses 0.07 μg/kg/day, i.m.); c) Hydrolysate - EWH (1 g/kg/day, gavage); d) Mercury and Hydrolysate. Mechanical allodynia was assessed using Von Frey Hairs test; heat hyperalgesia by the plantar test; catalepsy by a modification of the “ring test” and spontaneous locomotor activity by a photocell activity chambers. Analyses were performed at 0, 30 and 60 days of treatment. Brain and plasma MDA, plasma NPSH and TNF-α determination and skin immunohistochemistry were performed at 60 days. Hg induced a reduction in mechanical sensitivity threshold at 30 and 60 days and in thermal sensitivity threshold at 60 days. At the end of treatment catalepsy was developed, but there was not significant alteration in spontaneous locomotor activity. Hg also increased brain and plasma MDA, plasma NPSH and TNF-α levels and the number of Merkel cell–neurite complex in the skin. EWH prevented the development of mechanical allodynia, thermal hyperalgesia and catalepsy induced by Hg and the increase in MDA concentration in brain and plasma and in the number of Merkel cell–neurite complex in the skin. In conclusion, EWH promotes neuroprotection against the toxic effects caused by Hg, demonstrating a beneficial therapeutic potential., This research was supported by a Brazilian Government (Conselho Nacional de Desenvolvimento Científico e Tecnológico – CNPq – 203440/2014-5) and a Spanish Government (MINECO - AGL2012-32387; CSIC – Intramural201570I028).

Amino Acids, Peptides, and Proteins Essential Amino Acids M. C. Aristoy and F. Toldra Glutamine and Taurine C. G. Prosser and S. M. Rutherfurd Bioactive Peptides M. Miguel, B. Hernandez-Ledesma, R. Lopez-Fandino, and I. Recio Glutathione O. Demirkol and N. Ercal l-Carnitine J. Demarquoy, C. Rigault, and F. Le Borgne Creatine L. Mora and F. Toldra Analysis of Bioactive Peptides and Proteins P. Rupa and Y. Mine Vitamins Vitamin K X. Fu and S. L. Booth Methods for the Simultaneous Quantitative Analysis of Water-Soluble Vitamins in Food Products O. Heudi Tocopherols, Tocotrienols, and Their Bioactive Analogs S. Pacifico, M. Scognamiglio, B. D'Abrosca, P. Monaco, and A. Fiorentino Vitamin C O. Martin-Belloso, I. Odriozola-Serrano, and R. Soliva-Fortuny Vitamin D J. Jakobsen and R. B. Japelt Terpenes Hydrocarbon Carotenoids M. da Graca Dias Oxycarotenoids (Xanthophylls) D. Giuffrida, P. Dugo, P. Donato, G. Dugo, and L. Mondello Phenolic Compounds Flavonoids: Flavonols, Flavones, and Flavanones F. Ferreres and F. A. Tomas-Barberan Flavan--Ols and Proanthocyanidins S. Guyot Stilbenes and Resveratrol M. do Rosario Bronze, C. M. M. Duarte, and A. Matias Flavonoids: Anthocyanins V. Cheynier, C. Gomez, and A. Ageorges Isoflavones G. G. C. Kuhnle Hydrolyzable Tannins: Gallotannins Ellagitannins, and Ellagic Acid M. Jourdes, L. Pouysegu, S. Quideau, F. Mattivi, P. Truchado, and F. A. Tomas-Barberan Analysis of Chlorogenic Acids and Other Hydroxycinnamates in Food, Plants, and Pharmacokinetic Studies N. Kuhnert, H. Karakose, and R. Jaiswal Fibers and Polysaccharides Analytical Methodologies of Chitosan in Functional Foods Y. Liu, Y. Bai, and H. Liu Insoluble Dietary Fiber L. P. da Silva and M. Walter Fructans Including Inulin M. M. Jacob and S. G. Prapulla Pectin Extraction, Gelation, and Sources A. Rascon-Chu, V. U. Orona, A. Sanchez, and E. Carvajal-Millan Cyclodextrins K. Martina and G. Cravotto Probiotics The Selection and Evaluation of Probiotics G. Perez Martinez, C. Bauerl, and M. C. Collado Amores Phytoestrogens and Hormones Anise Oil L. M. L. Nollet Occurrence and Analysis of Melatonin in Food Plants M. Iriti, S. Vitalini, M. Rossoni, and F. Faoro Tetrapyrroles and Alkaloids Chlorophylls, Chlorophyll-Related Molecules, and Open-Chain Tetrapyrroles B. Schoefs Minerals and Trace Elements Minerals G. Garcia-Llatas, A. Alegria, R. Barbera, M. J. Lagarda, and R. Farre Lipid Compounds Omega and Omega Fatty Acids K. D. Stark Conjugated Linoleic Acid M. A. de la Fuente and M. Juarez Lecithin M. Leon-Camacho and M. Narvaez-Rivas Sterols H Saoussem Stanols H. Saoussem Analytical Methods for the Determination of alpha-Lipoic Acid, Dihydrolipoic Acid, and Lipoyllysine in Dietary Supplements and Foodstuffs G. Sontag and H. Schwartz Alliin and Allicin L. M. L. Nollet Sweeteners Methods of Analysis of Acesulfame-K and Aspartame G.-P. Nikoleli, A. G. Asimakopoulos, and D. P. Nikolelis Methods of Analysis of Saccharin A. G. Asimakopoulos, G.-P. Nikoleli, N. S. Thomaidis, and D. P. Nikolelis Salt Replacers and Taste Modifying Compounds Sodium Replacers M. Reig, M. Armenteros, M-C. Aristoy, and F. Toldra Triterpene Glycosides M. Ganzera Index