162 results on '"Martínez-Alonso, M."'
Search Results
2. Epidemiology of Psoriasis. A Population-Based Study
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Fernández-Armenteros, J.M., Gómez-Arbonés, X., Buti-Solé, M., Betriu-Bars, A., Sanmartin-Novell, V., Ortega-Bravo, M., Martínez-Alonso, M., and Casanova-Seuma, J.M.
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- 2019
- Full Text
- View/download PDF
3. Características epidemiológicas de la psoriasis. Un estudio poblacional
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Fernández-Armenteros, J.M., Gómez-Arbonés, X., Buti-Solé, M., Betriu-Bars, A., Sanmartin-Novell, V., Ortega-Bravo, M., Martínez-Alonso, M., and Casanova-Seuma, J.M.
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- 2019
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4. Molecular basis of dental sensitivity: The odontoblasts are multisensory cells and express multifunctional ion channels
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Solé-Magdalena, A., Martínez-Alonso, M., Coronado, C.A., Junquera, L.M., Cobo, J., and Vega, J.A.
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- 2018
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5. Pharmaceuticals removal and microbial community assessment in a continuous fungal treatment of non-sterile real hospital wastewater after a coagulation-flocculation pretreatment
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Mir-Tutusaus, J.A., Parladé, E., Llorca, M., Villagrasa, M., Barceló, D., Rodriguez-Mozaz, S., Martinez-Alonso, M., Gaju, N., Caminal, G., and Sarrà, M.
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- 2017
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6. Health-Related Quality of Life in Young Cocaine Users and Associated Factors
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ITINERE Investigators, Lozano, O. M., Domingo-Salvany, A., Martinez-Alonso, M., Brugal, M. T., Alonso, J., and de la Fuente, L.
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- 2008
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7. Informed choice in breast cancer screening: the role of education
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Universitat Rovira i Virgili, Pons-Rodríguez A., Martínez-Alonso M., Rué M., Perestelo-Pérez L., Sala M., Garcia M., Cardona À., ÀreaQ , Toledo A., Feijoo-Cid M., García M., Vidal C., Buil S., Viñals C., Viñals L., Ortega M., Pla S., Soler J., Carles-Lavila M., Pérez-Lacasta M.J., Pla R., Burón A., Castells X., Romero A., Codern N., Universitat Rovira i Virgili, and Pons-Rodríguez A., Martínez-Alonso M., Rué M., Perestelo-Pérez L., Sala M., Garcia M., Cardona À., ÀreaQ , Toledo A., Feijoo-Cid M., García M., Vidal C., Buil S., Viñals C., Viñals L., Ortega M., Pla S., Soler J., Carles-Lavila M., Pérez-Lacasta M.J., Pla R., Burón A., Castells X., Romero A., Codern N.
- Abstract
© 2020 SESPAS Objective: To evaluate the effect of receiving information about the benefits and harms of breast cancer screening in informed choice, according to educational level. Method: Secondary analysis of a randomized, controlled study, in four screening programs, in Catalonia and the Canary Islands (Spain). We analyzed 400 women who were going to be invited to participate for the first time. The intervention group received a decision aid that showed the benefits and harms of screening. The control group received a standard brochure that recommended participating in the screening program. Educational level was grouped into two categories, low and high. The primary outcome was informed choice defined as adequate knowledge and consistency between attitudes and intentions. Results: The intervention produced a greater increase in knowledge in women with a high educational level compared to those with a lower educational level. Among women who received the intervention, informed choice was almost three times higher in those with a high educational level (27% versus 11%). No differences were observed between educational levels in decisional conflict, confidence in the decision, anxiety and worry about breast cancer, in the intervention and control groups. Conclusions: A decision aid for breast cancer screening had much more impact on informed choice among women with a high educational level. In women with low educational level, the attitude towards screening improved and there was an increase in the intention to be screened.
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- 2021
8. Subcutaneous advanced glycation end-products and lung function according to glucose abnormalities: The ILERVAS Project
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Sánchez, E., Lecube, A., Betriu, À., Hernández, C., López-Cano, C., Gutiérrez-Carrasquilla, L., Kerkeni, M., Yeramian, A., Purroy, F., Pamplona, R., Farràs, C., Fernández, E., Barbé, F., Simó, R., Hernández, M., Rius, F., Polanco, D., de la Torre, M.S., Torres, G., Godoy, P., Portero-Otin, M., Jové, M., Colàs-Compàs, L., Benabdelhak, I., Miquel, E., Ortega, M., Valdivielso, J.M., Bermúdez, M., and Martínez-Alonso, M.
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- 2019
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9. Changes of resistance rates in Pseudomonas aeruginosa strains are unrelated to antimicrobial consumption in ICU populations with invasive device-related infection
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Álvarez-Lerma F, Olaechea-Astigarraga P, Gimeno R, Catalan M, Nuvials X, Gracia-Arnilla MP, Palomar-Martínez M, Seijas-Betolaza I, Martínez-Alonso M, and ENVIN-HELICS Study Group
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Antibiotic resistance, Antibióticos antipseudomonales, Antipseudomonal antimicrobials, Critically ill, ICU, Infecciones relacionadas con dispositivos invasivos, Invasive device-related infections (IDRI), Paciente crítico, Pseudomonas aeruginosa, Resistencia a antibióticos, Unidad de Cuidados Intensivos - Abstract
To evaluate the relationship between antipseudomonal antibiotic consumption and each individual drug resistance rate in Pseudomonas aeruginosa strains causing ICU acquired invasive device-related infections (IDRI).
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- 2019
10. La reputación digital como estrategia transformadora en las organizaciones militares
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Martínez Alonso, M. A. and Martínez Alonso, M. A.
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The key objective in the public relations strategy of any military organization is the generation of trust among its publics. For this, the attention and continuous improvement of its reputation is essential. The digital medium, in which organizations are subject to continuous scrutiny and which demands immediacy, success and coherence, has a great influence, also, on the offline world. Therefore, digital reputation becomes a strategic issue in relations with all its publics. In the Reputation Age, the use of digital reputation as a public relations strategy in military organizations is a necessary and essential process in the current environment that has long-term effects and that must be planned. It has transformative effects in the talent of the organization that imply desired changes in the corporate culture and, consequently, adapt the organization to the demands of today’s digital society., El objetivo clave en la estrategia de relaciones públicas de cualquier organización militar es la generación de confianza entre sus públicos. Para ello, la atención y la mejora continua de su reputación es indispensable. El medio digital, en el que las organizaciones se encuentran sometidas a un continuo escrutinio y que exige inmediatez, acierto y coherencia, tiene una gran influencia, también, en el mundo off line. Por ello, la reputación digital se convierte en una cuestión estratégica en las relaciones con todos sus públicos. En plena «era de la reputación», la utilización de la reputación digital como estrategia de relaciones públicas en las organizaciones militares es un proceso necesario e imprescindible en el entorno actual que tiene efectos a largo plazo y que debe ser planificado y programado. En el talento de la propia organización, tiene unos efectos transformadores que implican cambios deseados en la cultura corporativa y, en consecuencia, adapta a la organización a las exigencias de la sociedad digital actual.
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- 2019
11. Electroconvulsive therapy clinical database: Influence of age and gender on the electrical charge
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Salvador Sánchez J., David M.D., Torrent Setó A., Martínez Alonso M., Portella Moll M.J., Pifarré Paredero J., Vieta Pascual E., and Mur Laín M.
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Adult ,Male ,Adolescent ,Databases, Factual ,very elderly ,electroconvulsive therapy ,Young Adult ,Sex Factors ,data base ,middle aged ,gender ,Humans ,electricity ,human ,procedures ,Longitudinal Studies ,Prospective Studies ,hospital ,Aged ,Aged, 80 and over ,model ,statistical model ,longitudinal study ,Age Factors ,major clinical study ,female ,age ,sex factor ,factual database ,linear regression analysis ,Linear Models ,devices ,prospective study - Abstract
Introduction The influence of age and gender in the electrical charge delivered in a given population was analysed using an electroconvulsive therapy (ECT) clinical database. Material and method An observational, prospective, longitudinal study with descriptive analysis was performed using data from a database that included total bilateral frontotemporal ECT carried out with a Mecta spECTrum 5000Q® in our hospital over 6 years. From 2006 to 2012, a total of 4,337 ECT were performed on 187 patients. Linear regression using mixed effects analysis was weighted by the inverse of the number of ECT performed on each patient per year of treatment. Results The results indicate that age is related with changes in the required charge (P = .031), as such that the older the age a higher charge is needed. Gender is also associated with changes in charge (P = .014), with women requiring less charge than men, a mean of 87.3 mC less. When the effects of age and gender are included in the same model, both are significant (P = .0080 and P = .0041). Thus, for the same age, women require 99.0 mC less charge than men, and in both genders the charge increases by 2.3 mC per year. Conclusions From our study, it is concluded that the effect of age on the dosage of the electrical charge is even more significant when related to gender. It would be of interest to promote the systematic collection of data for a better understanding and application of the technique. © 2015 SEP y SEPB
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- 2017
12. The effect of information about the benefits and harms of mammography on women's decision-making: study protocol for a randomized controlled trial.
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Universitat Rovira i Virgili, Carles M, Martínez-Alonso M, Pons A, Pérez-Lacasta MJ, Perestelo-Pérez L, Sala M, Vidal C, Garcia M, Toledo-Chávarri A, Codern N, Feijoo-Cid M, Romero A, Pla R, Soler-González J, Castells X, Rué M, InforMa Group, Universitat Rovira i Virgili, and Carles M, Martínez-Alonso M, Pons A, Pérez-Lacasta MJ, Perestelo-Pérez L, Sala M, Vidal C, Garcia M, Toledo-Chávarri A, Codern N, Feijoo-Cid M, Romero A, Pla R, Soler-González J, Castells X, Rué M, InforMa Group
- Abstract
The decision to participate or not in breast cancer screening is complex due to the trade-off between the expected benefit of breast cancer mortality reduction and the major harm of overdiagnosis. It seems ethically necessary to inform women so that they can actively participate in decision-making and make an informed choice based on their values and preferences. The objective of this study is to assess the effects of receiving information about the benefits and harms of screening on decision-making, in women approaching the age of invitation to mammography screening.A two-stage, randomized controlled trial (RCT). In the first stage, 40 Basic Health Areas (BHAs) will be selected and randomized to intervention or control. In the second stage, women within each BHA will be randomly selected (n?=?400). Four breast cancer screening programs (BCSPs) of the Spanish public health system, three in Catalonia and one in the Canary Islands will participate in the study. Women in the intervention arm will receive a leaflet with detailed information on the benefits and harms of screening using mammography. Women in the control arm will receive a standard leaflet that does not mention harms and recommends accepting the invitation to participate in the biennial examinations of the BCSP. The primary outcome is informed choice, a dichotomous variable that combines knowledge, attitudes, and intentions. Secondary outcomes include decisional conflict; confidence in the decision made; anxiety about screening participation; worry about breast cancer; anticipated regret; time perspective; perceived importance of benefits/harms of screening; perceived risk of breast cancer; and leaflet acceptability. Primary and secondary outcomes are assessed 2-3 weeks after the intervention.This is the first
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- 2017
13. Changes of resistance rates in Pseudomonas aeruginosastrains are unrelated to antimicrobial consumption in ICU populations with invasive device-related infection
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Álvarez-Lerma, F., Olaechea-Astigarraga, P., Gimeno, R., Catalan, M., Nuvials, X., Gracia-Arnilla, M.P., Palomar-Martínez, M., Seijas-Betolaza, I., and Martínez-Alonso, M.
- Abstract
To evaluate the relationship between antipseudomonal antibiotic consumption and each individual drug resistance rate in Pseudomonas aeruginosastrains causing ICU acquired invasive device-related infections (IDRI).
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- 2020
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14. Psoriasis, metabolic syndrome and cardiovascular risk factors. A population‐based study.
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Martínez‐Alonso, M., Garí, E., Portero‐Otín, M., Fernández‐Armenteros, J.M., Casanova‐Seuma, J.M., Gómez‐Arbonés, X., Betriu‐Bars, A., Sanmartin‐Novell, V., Buti‐Soler, M., Ortega‐Bravo, M., and Santamaria‐Babi, L.
- Abstract
Background: Psoriasis is a very prevalent systemic chronic inflammatory disease. Major cardiovascular events are the main cause of mortality in these patients which suggests an association between psoriasis and traditional cardiovascular risk factors. Objective: To identify classic cardiovascular risk factors and metabolic syndrome (MS) in patients with psoriasis, their possible association with its severity and compare it with the non‐psoriatic population. Methods: This is an observational and cross‐sectional population study in Lleida (Spain) from a joint hospital/primary care database. Results: The database comprised 398 701 individuals. There were 6868 cases registered as psoriasis (1.7%), and 499 of them (7.3%) were classified as moderate–severe psoriasis. Patients with psoriasis had a higher prevalence of traditional cardiovascular risk factors than non‐psoriatic population: diabetes mellitus 2 (13.9% vs 7.4%, OR 2.01), dyslipidaemia (28.8% vs 17.4%, OR 1.92), arterial hypertension (31.2% vs 19.0%, OR 1.93), obesity (33.7% vs 28.1%, OR 1.30), altered fasting basal glycaemia (21.4% vs 15.1%, OR 1.54), low cholesterol HDL (38.1% vs 32.3%, OR 1.29), hypertriglyceridaemia (45.7% vs 35.2%, OR 1.55) and high waist circumference (75.7% vs 72.3%, OR 1.19). MS was more prevalent in psoriatic patients (28.3% vs 15.1%, OR 2.21), and cardiovascular risk factors were similar between psoriasis severity groups. Psoriatic patients had a higher prevalence of ischaemic heart disease (3.3% vs 1.8%, OR 1.87) and vascular cerebral accidents (1.8% vs 1.2%, OR 1.55). A model for MS showed a significant nonlinear relationship with age and sex and significant differences between patients with and without psoriasis. Conclusion: We found statistically significant differences in relation to the prevalence of cardiovascular risk factors, MS and major cardiovascular events in psoriatic patients. However, differences were not seen between psoriasis severity groups. Our work reinforces the need for a multidisciplinary approach and close monitoring of cardiovascular risk factors in these patients to prevent a cardiovascular event. [ABSTRACT FROM AUTHOR]
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- 2019
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15. Impact of risk factors on different interval cancer subtypes in a population-based breast cancer screening programme
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Pla, R., Pérez, M.J., Gregori, A., Carles, M., Vilaprinyó, E., Roso, A., Martínez-Alonso, M., Forné, C., Puig-Vives, M., Marcos-Gragera, R., Renart, G., Castanyer, F., Torà, N., Baré, M., Sarriugarte, G., Reyes, J.M., De La Lastra, I.D., Vázquez, M., Sarandeses, A., Mosquera, J., Fernández, A.B., Corujo, M., Conde, M., Almazán, R., Torrella, A., Morales, J., Miranda, J., Martínez, I., Lluch, A., Casals, M., Alberich, C., Romero, A., Román, M., Rodríguez-Arana, A., Martínez, J., Macià, F., Comas, M., Salas, D., Rué, M., Castells, X., Ferrer, J., Zubizarreta, R., Barata, T., Otegi, A., Fernandez, B., Domingo, L., Ibáñez, J., Sala, M., Blanch, J., Psicologia, and Universitat Rovira i Virgili.
- Abstract
10.1371/journal.pone.0110207 Background Interval cancers are primary breast cancers diagnosed in women after a negative screening test and before the next screening invitation. Our aim was to evaluate risk factors for interval cancer and their subtypes and to compare the risk factors identified with those associated with incident screen-detected cancers. Methods We analyzed data from 645,764 women participating in the Spanish breast cancer screening program from 2000¿2006 and followed-up until 2009. A total of 5,309 screen-detected and 1,653 interval cancers were diagnosed. Among the latter, 1,012 could be classified on the basis of findings in screening and diagnostic mammograms, consisting of 489 true interval cancers (48.2%), 235 false-negatives (23.2%), 172 minimal-signs (17.2%) and 114 occult tumors (11.3%). Information on the screening protocol and women's characteristics were obtained from the screening program registry. Cause-specific Cox regression models were used to estimate the hazard ratios (HR) of risks factors for interval cancer and incident screen-detected cancer. A multinomial regression model, using screen-detected tumors as a reference group, was used to assess the effect of breast density and other factors on the occurrence of interval cancer subtypes. Results A previous false-positive was the main risk factor for interval cancer (HR = 2.71, 95%CI: 2.28¿3.23); this risk was higher for false-negatives (HR = 8.79, 95%CI: 6.24¿12.40) than for true interval cancer (HR = 2.26, 95%CI: 1.59¿3.21). A family history of breast cancer was associated with true intervals (HR = 2.11, 95%CI: 1.60¿2.78), previous benign biopsy with a false-negatives (HR = 1.83, 95%CI: 1.23¿2.71). High breast density was mainly associated with occult tumors (RRR = 4.92, 95%CI: 2.58¿
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- 2014
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16. Identificación de suelos supresivos a Meloidogyne spp. en parcelas de producción comercial de hortalizas bajo plástico
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Giné Blasco, Ariadna, Martínez Alonso, M., Gaju Ricart, Nuria, Ornat Longarón, Cèsar, Sorribas Royo, Francisco Javier, Universitat Politècnica de Catalunya. Departament d'Enginyeria Agroalimentària i Biotecnologia, and Universitat Politècnica de Catalunya. PV - Patologia Vegetal
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Enginyeria agroalimentària::Agricultura::Fitopatologia [Àrees temàtiques de la UPC] ,Meloidogyne ,Fitopatologia - Abstract
Dos invernaderos de producción ecológica localizados en Tarragona (A) y Amposta (B) fueron monitorizados desde 10/2010 hasta 7/2013 para determinar la fluctuación de la población de Meloidogyne spp., el porcentaje de huevos parasitados por hongos y las especies fúngicas implicadas. Al inicio del estudio el porcentaje de parasitismo era del 14 y 60% en A y B, respectivamente; y las raíces mostraban niveles bajos de agallamiento para el tipo de cultivo y densidad de población en pretrasplante, sugiriendo que podrían ser suelos supresivos a la enfermedad.
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- 2014
17. Identificación de suelos supresivos a Meloidogyne spp. en parcelas de producción comercial de hortalizas bajo plástico
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Universitat Politècnica de Catalunya. Departament d'Enginyeria Agroalimentària i Biotecnologia, Universitat Politècnica de Catalunya. PV - Patologia Vegetal, Giné Blasco, Ariadna, Martínez Alonso, M., Gaju Ricart, Nuria, Ornat Longarón, Cèsar, Sorribas Royo, Francisco Javier, Universitat Politècnica de Catalunya. Departament d'Enginyeria Agroalimentària i Biotecnologia, Universitat Politècnica de Catalunya. PV - Patologia Vegetal, Giné Blasco, Ariadna, Martínez Alonso, M., Gaju Ricart, Nuria, Ornat Longarón, Cèsar, and Sorribas Royo, Francisco Javier
- Abstract
Dos invernaderos de producción ecológica localizados en Tarragona (A) y Amposta (B) fueron monitorizados desde 10/2010 hasta 7/2013 para determinar la fluctuación de la población de Meloidogyne spp., el porcentaje de huevos parasitados por hongos y las especies fúngicas implicadas. Al inicio del estudio el porcentaje de parasitismo era del 14 y 60% en A y B, respectivamente; y las raíces mostraban niveles bajos de agallamiento para el tipo de cultivo y densidad de población en pretrasplante, sugiriendo que podrían ser suelos supresivos a la enfermedad., Postprint (published version)
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- 2014
18. Impact of risk factors on different interval cancer subtypes in a population-based breast cancer screening programme
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Psicologia, Universitat Rovira i Virgili., Pla, R., Pérez, M.J., Gregori, A., Carles, M., Vilaprinyó, E., Roso, A., Martínez-Alonso, M., Forné, C., Puig-Vives, M., Marcos-Gragera, R., Renart, G., Castanyer, F., Torà, N., Baré, M., Sarriugarte, G., Reyes, J.M., De La Lastra, I.D., Vázquez, M., Sarandeses, A., Mosquera, J., Fernández, A.B., Corujo, M., Conde, M., Almazán, R., Torrella, A., Morales, J., Miranda, J., Martínez, I., Lluch, A., Casals, M., Alberich, C., Romero, A., Román, M., Rodríguez-Arana, A., Martínez, J., Macià, F., Comas, M., Salas, D., Rué, M., Castells, X., Ferrer, J., Zubizarreta, R., Barata, T., Otegi, A., Fernandez, B., Domingo, L., Ibáñez, J., Sala, M., Blanch, J., Psicologia, Universitat Rovira i Virgili., Pla, R., Pérez, M.J., Gregori, A., Carles, M., Vilaprinyó, E., Roso, A., Martínez-Alonso, M., Forné, C., Puig-Vives, M., Marcos-Gragera, R., Renart, G., Castanyer, F., Torà, N., Baré, M., Sarriugarte, G., Reyes, J.M., De La Lastra, I.D., Vázquez, M., Sarandeses, A., Mosquera, J., Fernández, A.B., Corujo, M., Conde, M., Almazán, R., Torrella, A., Morales, J., Miranda, J., Martínez, I., Lluch, A., Casals, M., Alberich, C., Romero, A., Román, M., Rodríguez-Arana, A., Martínez, J., Macià, F., Comas, M., Salas, D., Rué, M., Castells, X., Ferrer, J., Zubizarreta, R., Barata, T., Otegi, A., Fernandez, B., Domingo, L., Ibáñez, J., Sala, M., and Blanch, J.
- Abstract
10.1371/journal.pone.0110207, Background Interval cancers are primary breast cancers diagnosed in women after a negative screening test and before the next screening invitation. Our aim was to evaluate risk factors for interval cancer and their subtypes and to compare the risk factors identified with those associated with incident screen-detected cancers. Methods We analyzed data from 645,764 women participating in the Spanish breast cancer screening program from 2000¿2006 and followed-up until 2009. A total of 5,309 screen-detected and 1,653 interval cancers were diagnosed. Among the latter, 1,012 could be classified on the basis of findings in screening and diagnostic mammograms, consisting of 489 true interval cancers (48.2%), 235 false-negatives (23.2%), 172 minimal-signs (17.2%) and 114 occult tumors (11.3%). Information on the screening protocol and women's characteristics were obtained from the screening program registry. Cause-specific Cox regression models were used to estimate the hazard ratios (HR) of risks factors for interval cancer and incident screen-detected cancer. A multinomial regression model, using screen-detected tumors as a reference group, was used to assess the effect of breast density and other factors on the occurrence of interval cancer subtypes. Results A previous false-positive was the main risk factor for interval cancer (HR = 2.71, 95%CI: 2.28¿3.23); this risk was higher for false-negatives (HR = 8.79, 95%CI: 6.24¿12.40) than for true interval cancer (HR = 2.26, 95%CI: 1.59¿3.21). A family history of breast cancer was associated with true intervals (HR = 2.11, 95%CI: 1.60¿2.78), previous benign biopsy with a false-negatives (HR = 1.83, 95%CI: 1.23¿2.71). High breast density was mainly associated with occult tumors (RRR = 4.92, 95%CI: 2.58¿
- Published
- 2014
19. Estudio de la población nacida pequeña para la edad gestacional en tratamiento con hormona del crecimiento.
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Muñoz, J. Bosch, Godoy, L. Marfil, Martínez-Alonso, M., Cabello, J. J. Trujillano, and Mir, E. Solé
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- 2016
20. Stable Carbon Isotope Fractionation During 1,2-Dichloropropane-to-Propene Transformation by an Enrichment Culture Containing Dehalogenimonas Strains and a dcpA Gene.
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Martín-González, L., Hatijah Mortan, S., Rosell, M., Parladé, E., Martínez-Alonso, M., Gaju, N., Caminal, G., Adrian, L., and Marco-Urrea, E.
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- 2015
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21. Trastorno de la comunicación social (pragmático), nueva categoría diagnóstica DSM-5, consideraciones clínicas y diagnóstico diferencial a propósito de un caso.
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Martínez Alonso, M. B., Fernández Rodríguez, M. M., and Pérez Moleiro, L.
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Definition: social (pragmatic) communication disorder is a new diagnostic entity included in the DSM-5. It is characterized by persistent difficulties in the social use of verbal and nonverbal communication, differing from autism spectrum disorder by the absence of restricted interests and/or stereotyped activities1. Case report: we report the case of a 14-year-old girl, with a family history of autism spectrum disorder (ASD). She was referred to the child and adolescent psychiatry clinic because of reactive anxiety secondary to bulling at school. On examination, her main problem was centered on communication and social relationships. She meets diagnostic criteria for social communication disorder. Conclusion: adolescence can be considered a critical period for patients with social communication disorder. Early diagnosis is essential, and the mainstay of treatment is a program of social skills training. [ABSTRACT FROM AUTHOR]
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- 2015
22. Effect of continuous positive airway pressure on the incidence of hypertension and cardiovascular events in nonsleepy patients with obstructive sleep apnea: a randomized controlled trial.
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Barbé F, Durán-Cantolla J, Sánchez-de-la-Torre M, Martínez-Alonso M, Carmona C, Barceló A, Chiner E, Masa JF, Gonzalez M, Marín JM, Garcia-Rio F, Diaz de Atauri J, Terán J, Mayos M, de la Peña M, Monasterio C, del Campo F, Montserrat JM, Spanish Sleep And Breathing Network, and Barbé, Ferran
- Abstract
Context: Continuous positive airway pressure (CPAP) is the first-line treatment for patients with symptomatic obstructive sleep apnea (OSA). However, its indication for all patients with sleep-disordered breathing, regardless of daytime symptoms, is unclear.Objective: To evaluate the effect of CPAP treatment on the incidence of hypertension or cardiovascular events in a cohort of nonsleepy patients with OSA.Design, Setting, and Patients: Multicenter, parallel-group, randomized controlled trial in 14 teaching hospitals in Spain. Between May 2004 and May 2006, 725 consecutive patients were enrolled who had an apnea-hypopnea index of 20 h(-1) or greater and an Epworth Sleepiness Scale score of 10 or less (scores range from 0-24, with values <10 suggesting no daytime sleepiness). Exclusion criteria were previous cardiovascular event, physical or psychological incapacity, chronic disease, or drug or alcohol addiction. Follow-up ended in May 2009.Intervention: Patients were allocated to receive CPAP treatment or no active intervention. All participants received dietary counseling and sleep hygiene advice.Main Outcome Measures: Incidence of either systemic hypertension (taking antihypertensive medication or blood pressure greater than 140/90 mm Hg) or cardiovascular event (nonfatal myocardial infarction, nonfatal stroke, transient ischemic attack, hospitalization for unstable angina or arrhythmia, heart failure, or cardiovascular death).Results: Seven hundred twenty-three patients underwent follow-up for a median of 4 (interquartile range, 2.7-4.4) years (1 patient from each group did not receive allocated treatment); 357 in the CPAP group and 366 in the control group were included in the analysis. In the CPAP group there were 68 patients with new hypertension and 28 cardiovascular events (17 unstable angina or arrhythmia, 3 nonfatal stroke, 3 heart failure, 2 nonfatal myocardial infarction, 2 transient ischemic attack, 1 cardiovascular death). In the control group there were 79 patients with new hypertension and 31 cardiovascular events (11 unstable angina or arrhythmia, 8 nonfatal myocardial infarction, 5 transient ischemic attack, 5 heart failure, 2 nonfatal stroke). The hypertension or cardiovascular event incidence density rate was 9.20 per 100 person-years (95% CI, 7.36-11.04) in the CPAP group and 11.02 per 100 person-years (95% CI, 8.96-13.08) in the control group. The incidence density ratio was 0.83 (95% CI, 0.63-1.1; P = .20).Conclusions: In patients with OSA without daytime sleepiness, the prescription of CPAP compared with usual care did not result in a statistically significant reduction in the incidence of hypertension or cardiovascular events. However, the study may have had limited power to detect a significant difference.Trial Registration: clinicaltrials.gov Identifier: NCT00127348. [ABSTRACT FROM AUTHOR]- Published
- 2012
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23. Morphological and ultrastructural characterization of an unusual purple sulfur bacterium from a marine microbial-mat community
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Martínez-Alonso, M., Mir, J., Gaju, N., and Esteve, I.
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- *
MICROBIAL mats , *SULFUR bacteria , *CHROMATIACEAE , *RHODOSPIRILLALES - Abstract
Abstract: An unusual purple sulfur bacterium present in the pink layer of the Ebro Delta microbial mats has been identified through the study of its ultrastructural features. As pure cultures of this bacterium have not been obtained, due to its inability to grow in axenic conditions, only enrichment cultures where it reached at least 90% of total biomass have been considered. In enrichment cultures, the cells are rods that are 5.4±0.6μm wide and 11.0±2.1μm long. The color of the cell suspensions is pink to pinkish-red. Cells are motile by means of a polar tuft of flagella and multiply by binary fission. This bacterium possesses an extensive internal photosynthetic membrane system consisting of stacks of lamellae, contains bacteriochlorophyll a and carotenoids of normal spirilloxanthin series and deposits sulfur intracellularly. In natural samples, the most abundant phototrophic purple sulfur bacterium developing in this ecosystem has the same kind of intracytoplasmic membrane system, but the cells differ slightly in size and arrangement, in that they are ovoid, 4.2±0.5μm wide and 6.0±1.4μm long, and can be seen forming irregular non-motile clumps which are embedded in slime. Differences observed between field samples and enrichment cultures suggest that environmental conditions may bring about changes in the phenotypic characteristics of the cells. The morphological characteristics of the described bacterium resemble those of large Chromatia. However, this bacterium differs from known species in this group, particularly in terms of its photosynthetic membrane system and in its light absorption properties. [Copyright &y& Elsevier]
- Published
- 2006
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24. High quality recombinant protein production in insect cell expression system by bacterial chaperones
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Miralles, N. Ferrer, Martínez-Alonso, M., Noad, R., Roy, P., Villaverde, A., and Ferrer-Miralles, N.
- Published
- 2009
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25. Distribution of phototrophic populations and primary production in a microbial mat from the Ebro Delta, Spain
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Martínez-Alonso, M., Mir, J., Caumette, P., Nuria Gaju, Guerrero, R., and Esteve, I.
- Subjects
anoxygenic photosynthesis ,phototrophic bacteria ,microbial mats ,oxygenic photosynthesis - Abstract
Microbial mats arising in the sand flats of the Ebro Delta (Tarragona, Spain) were investigated during the summer season, when the community was highly developed. These mats are composed of three pigmented layers of phototrophic organisms, an upper brown layer mainly composed of Lyngbya aestuarii and diatoms, an intermediate green layer of the cyanobacterium Microcoleus chthonoplastes, and an underlying pink layer of a so-far unidentified purple sulfur bacterium. In the photic zone, oxygenic phototrophs constitute about 58% of total photosynthetic biomass, measured as biovolume, and anoxygenic phototrophs represent 42%. Diatoms constitute 11.8% of the oxygenic biomass, M. chthonoplastes 61.2%, and L. aestuarii and coccoid cyanobacteria 20.6 and 6.4%, respectively. In this laminated community, organic matter has an autochthonous origin, and photosynthesis is the most important source of organic carbon. Oxygen production reaches up to 27.2 mmol O2 m-2 h-1, measured at 1000 µE m-2 s-1 light intensity, whereas oxidation of sulfide in the light has been calculated to be 18.6 mmol S m-2 h-1. This amount represents 26% of the total photosynthetic production in terms of photoassimilated carbon, demonstrating the important role of anoxygenic phototrophs as primary producers in the pink layer of Ebro Delta microbial mats.
26. Seasonal microbial ribotype shifts in the sulfurous karstic lakes Cisó and Vilar, in northeastern Spain
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Ramírez-Moreno, S., Martínez-Alonso, M., Méndez-Álvarez, S., and Nuria Gaju
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16S rRNA genes ,Domain Bacteria ,sulfurous lakes ,multidimensional scaling (MDS) ,seasonal shift ,restriction fragment length polymorphism (RFLP) - Abstract
Spatio-temporal changes in two sulfurous lakes from the karstic area of Banyoles (Girona, Spain), holomictic lake Cisó and meromictic lake Vilar, were studied over one year. Samples were collected at different depths from the two lakes on the same days, during each of the four seasons, and several physico-chemical variables (temperature, light, pH, conductivity, sulfide, oxygen concentration, pigment concentrations, etc.) were measured. To fingerprint bacterial populations from each sample, DNA was extracted, bacterial 16S rRNA genes were amplified by PCR, and restriction fragment length polymorphism (RFLP) analyses of the total bacterial 16S rDNAs were performed. Each 16S rDNA pool was independently digested with three restriction endonucleases (AluI, HinfI, and RsaI) and separated electrophoretically. More restriction fragments were obtained from the Lake Vilar samples than from the Lake Cisó samples. Moreover, intrasample and intersample differences were observed in each lake. RFLP patterns were compared by scoring similarities using the Jaccard coefficient and then building a multidimensional scaling (MDS) map from the resulting similarities matrix. In both lakes, results indicated that seasonality was mostly responsible for the observed fluctuations in the RFLP patterns, while the effect of stratification was less pronounced.
27. Competing risks to breast cancer mortality in Catalonia.
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Vilaprinyo E, Gispert R, Martínez-Alonso M, Carles M, Pla R, Espinàs JA, Rué M, Vilaprinyo, Ester, Gispert, Rosa, Martínez-Alonso, Montserrat, Carles, Misericòrdia, Pla, Roger, Espinàs, Josep-Alfons, and Rué, Montserrat
- Abstract
Background: Breast cancer mortality has experienced important changes over the last century. Breast cancer occurs in the presence of other competing risks which can influence breast cancer incidence and mortality trends. The aim of the present work is: 1) to assess the impact of breast cancer deaths among mortality from all causes in Catalonia (Spain), by age and birth cohort and 2) to estimate the risk of death from other causes than breast cancer, one of the inputs needed to model breast cancer mortality reduction due to screening or therapeutic interventions.Methods: The multi-decrement life table methodology was used. First, all-cause mortality probabilities were obtained by age and cohort. Then mortality probability for breast cancer was subtracted from the all-cause mortality probabilities to obtain cohort life tables for causes other than breast cancer. These life tables, on one hand, provide an estimate of the risk of dying from competing risks, and on the other hand, permit to assess the impact of breast cancer deaths on all-cause mortality using the ratio of the probability of death for causes other than breast cancer by the all-cause probability of death.Results: There was an increasing impact of breast cancer on mortality in the first part of the 20th century, with a peak for cohorts born in 1945-54 in the 40-49 age groups (for which approximately 24% of mortality was due to breast cancer). Even though for cohorts born after 1955 there was only information for women under 50, it is also important to note that the impact of breast cancer on all-cause mortality decreased for those cohorts.Conclusion: We have quantified the effect of removing breast cancer mortality in different age groups and birth cohorts. Our results are consistent with US findings. We also have obtained an estimate of the risk of dying from competing-causes mortality, which will be used in the assessment of the effect of mammography screening on breast cancer mortality in Catalonia. [ABSTRACT FROM AUTHOR]- Published
- 2008
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28. Impacto de los trastornos del ánimo, de ansiedad y de las enfermedades físicas crónicas en la calidad de vida de la población general de España. Resultados del estudio ESEMeD-España.
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Pinto-Meza, A., Haro, J. M., Palacín, C., Torres, J. V., Ochoa, S., Vilagut, G., Martínez-Alonso, M., Codony, M., and Alonso, J.
- Subjects
- *
AFFECTIVE disorders , *ANXIETY disorders , *QUALITY of life , *SPANIARDS , *EPIDEMIOLOGY , *MENTAL illness , *HEALTH - Abstract
Introduction. Mental disorders and chronic physical conditions significantly impair health related quality of life (HRQOL). To date, there are no studies in the general population of Spain about their impact. The aim of the present study is to evaluate the impact of mood and anxiety disorders and chronic physical conditions in HRQOL and functional disability (estimated considering work loss days). Methods. The ESEMeD-Spain is an epidemiological study carried out in the general population of Spain aged 18 years or older. Mental disorders were assessed with the Composite International Diagnostic Interview (CIDI 3.0); the HRQOL with the SF-12; and functional disability with the WHO Disability Assessment Schedule. Additionally, chronic physical conditions were assessed. Results. A total of 5,473 individuals were assessed. Response rate was 78.6 %. Mental disorders, specially mood disorders, showed the highest impairment in HRQOL and functional disability (more work loss days). This impairment was even higher than the impairment associated to chronic physical conditions. Comorbidity between mood and anxiety disorders was associated to the worst HRQOL. In general, mental HRQOL was more impaired than physical HRQOL. Conclusions. Mood disorders substantially impair HRQOL and augment functional disability in Spain. Their comorbidity with anxiety disorders in especially impairing. [ABSTRACT FROM AUTHOR]
- Published
- 2007
29. Bombesin-Targeted Delivery of β-Carboline-Based Ir(III) and Ru(II) Photosensitizers for a Selective Photodynamic Therapy of Prostate Cancer.
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Sanz-Villafruela J, Bermejo-Casadesús C, Riesco-Llach G, Iglesias M, Martínez-Alonso M, Planas M, Feliu L, Espino G, and Massaguer A
- Subjects
- Humans, Male, Coordination Complexes chemistry, Coordination Complexes pharmacology, Coordination Complexes chemical synthesis, Drug Screening Assays, Antitumor, Molecular Structure, Cell Survival drug effects, Apoptosis drug effects, Cell Line, Tumor, Cell Proliferation drug effects, Photosensitizing Agents chemistry, Photosensitizing Agents pharmacology, Photosensitizing Agents chemical synthesis, Photochemotherapy, Iridium chemistry, Iridium pharmacology, Prostatic Neoplasms drug therapy, Prostatic Neoplasms pathology, Ruthenium chemistry, Ruthenium pharmacology, Carbolines chemistry, Carbolines pharmacology, Carbolines chemical synthesis, Antineoplastic Agents chemistry, Antineoplastic Agents pharmacology, Antineoplastic Agents chemical synthesis, Bombesin chemistry, Bombesin pharmacology
- Abstract
Despite advances in Ir(III) and Ru(II) photosensitizers (PSs), their lack of selectivity for cancer cells has hindered their use in photodynamic therapy (PDT). We disclose the synthesis and characterization of two pairs of Ir(III) and Ru(II) polypyridyl complexes bearing two β-carboline ligands (N^N') functionalized with -COOMe ( L1 ) or -COOH ( L2 ), resulting in PSs of formulas [Ir(C^N)
2 (N^N')]Cl ( Ir-Me: C^N = ppy, N^N' = L1 ; Ir-H: C^N = ppy, N^N' = L2 ) and [Ru(N^N)2 (N^N')](Cl)2 ( Ru-Me: N^N = bpy, N^N' = L1; Ru-H: N^N = bpy, N^N' = L2 ). To enhance their selectivity toward cancer cells, Ir-H and Ru-H were coupled to a bombesin derivative ( BN3 ), resulting in the metallopeptides Ir-BN and Ru-BN . Ir(III) complexes showed higher anticancer activity than their Ru(II) counterparts, particularly upon blue light irradiation, but lacked cancer cell selectivity. In contrast, Ir-BN and Ru-BN exhibited selective photocytoxicity against prostate cancer cells, with a lower effect against nonmalignant fibroblasts. All compounds generated ROS and induced severe mitochondrial toxicity upon photoactivation, leading to apoptosis. Additionally, the ability of Ir-Me to oxidize NADH was demonstrated, suggesting a mechanism for mitochondrial damage. Our findings indicated that the conjugation of metal PSs with BN3 creates efficient PDT agents, achieving selectivity through targeting bombesin receptors and local photoactivation.- Published
- 2024
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30. Insights into the anticancer photodynamic activity of Ir(III) and Ru(II) polypyridyl complexes bearing β-carboline ligands.
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Sanz-Villafruela J, Bermejo-Casadesus C, Zafon E, Martínez-Alonso M, Durá G, Heras A, Soriano-Díaz I, Giussani A, Ortí E, Tebar F, Espino G, and Massaguer A
- Subjects
- Humans, Ligands, Molecular Structure, Structure-Activity Relationship, Cell Proliferation drug effects, Pyridines chemistry, Pyridines pharmacology, Pyridines chemical synthesis, Reactive Oxygen Species metabolism, Dose-Response Relationship, Drug, Cell Line, Tumor, Cell Survival drug effects, Apoptosis drug effects, Iridium chemistry, Iridium pharmacology, Ruthenium chemistry, Ruthenium pharmacology, Antineoplastic Agents pharmacology, Antineoplastic Agents chemistry, Antineoplastic Agents chemical synthesis, Photosensitizing Agents pharmacology, Photosensitizing Agents chemistry, Photosensitizing Agents chemical synthesis, Coordination Complexes pharmacology, Coordination Complexes chemistry, Coordination Complexes chemical synthesis, Photochemotherapy, Carbolines chemistry, Carbolines pharmacology, Carbolines chemical synthesis, Drug Screening Assays, Antitumor
- Abstract
Ir(III) and Ru(II) polypyridyl complexes are promising photosensitizers (PSs) for photodynamic therapy (PDT) due to their outstanding photophysical properties. Herein, one series of cyclometallated Ir(III) complexes and two series of Ru(II) polypyridyl derivatives bearing three different thiazolyl-β-carboline N^N' ligands have been synthesized, aiming to evaluate the impact of the different metal fragments ([Ir(C^N)
2 ]+ or [Ru(N^N)2 ]2+ ) and N^N' ligands on the photophysical and biological properties. All the compounds exhibit remarkable photostability under blue-light irradiation and are emissive (605 < λem < 720 nm), with the Ru(II) derivatives displaying higher photoluminescence quantum yields and longer excited state lifetimes. The Ir PSs display pKa values between 5.9 and 7.9, whereas their Ru counterparts are less acidic (pKa > 9.3). The presence of the deprotonated form in the Ir-PSs favours the generation of reactive oxygen species (ROS) since, according to theoretical calculations, it features a low-lying ligand-centered triplet excited state (T1 =3 LC) with a long lifetime. All compounds have demonstrated anticancer activity. Ir(III) complexes 1-3 exhibit the highest cytotoxicity in dark conditions, comparable to cisplatin. Their activity is notably enhanced by blue-light irradiation, resulting in nanomolar IC50 values and phototoxicity indexes (PIs) between 70 and 201 in different cancer cell lines. The Ir(III) PSs are also activated by green (with PI between 16 and 19.2) and red light in the case of complex 3 (PI = 8.5). Their antitumor efficacy is confirmed by clonogenic assays and using spheroid models. The Ir(III) complexes rapidly enter cells, accumulating in mitochondria and lysosomes. Upon photoactivation, they generate ROS, leading to mitochondrial dysfunction and lysosomal damage and ultimately cell apoptosis. Additionally, they inhibit cancer cell migration, a crucial step in metastasis. In contrast, Ru(II) complex 6 exhibits moderate mitochondrial activity. Overall, Ir(III) complexes 1-3 show potential for selective light-controlled cancer treatment, providing an alternative mechanism to chemotherapy and the ability to inhibit lethal cancer cell dissemination., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)- Published
- 2024
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31. Hybrid Hydroxyapatite-Metal Complex Materials Derived from Amino Acids and Nucleobases.
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Jiménez-Pérez A, Martínez-Alonso M, and García-Tojal J
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- Coordination Complexes chemistry, Biocompatible Materials chemistry, Metals chemistry, Humans, Nucleic Acids chemistry, Durapatite chemistry, Amino Acids chemistry
- Abstract
Calcium phosphates (CaPs) and their substituted derivatives encompass a large number of compounds with a vast presence in nature that have aroused a great interest for decades. In particular, hydroxyapatite (HAp, Ca
10 (OH)2 (PO4 )6 ) is the most abundant CaP mineral and is significant in the biological world, at least in part due to being a major compound in bones and teeth. HAp exhibits excellent properties, such as safety, stability, hardness, biocompatibility, and osteoconductivity, among others. Even some of its drawbacks, such as its fragility, can be redirected thanks to another essential feature: its great versatility. This is based on the compound's tendency to undergo substitutions of its constituent ions and to incorporate or anchor new molecules on its surface and pores. Thus, its affinity for biomolecules makes it an optimal compound for multiple applications, mainly, but not only, in biological and biomedical fields. The present review provides a chemical and structural context to explain the affinity of HAp for biomolecules such as proteins and nucleic acids to generate hybrid materials. A size-dependent criterium of increasing complexity is applied, ranging from amino acids/nucleobases to the corresponding macromolecules. The incorporation of metal ions or metal complexes into these functionalized compounds is also discussed.- Published
- 2024
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32. Towards efficient Ir(III) anticancer photodynamic therapy agents by extending π-conjugation on N^N ligands.
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Sanz-Villafruela J, Bermejo-Casadesús C, Martínez-Alonso M, Moro A, Lima JC, Massaguer A, and Espino G
- Subjects
- Humans, Ligands, Drug Screening Assays, Antitumor, Cell Proliferation drug effects, Molecular Structure, Cell Survival drug effects, Cell Line, Tumor, Light, Density Functional Theory, Photochemotherapy, Iridium chemistry, Iridium pharmacology, Antineoplastic Agents chemistry, Antineoplastic Agents pharmacology, Antineoplastic Agents chemical synthesis, Photosensitizing Agents chemistry, Photosensitizing Agents pharmacology, Photosensitizing Agents chemical synthesis, Coordination Complexes chemistry, Coordination Complexes pharmacology, Coordination Complexes chemical synthesis
- Abstract
In this work we disclose a new family of biscyclometallated Ir(III) complexes of the general formula [Ir(C^N)
2 (N^N)]Cl (IrL1-IrL5), where HC^N is 1-phenyl-β-carboline and N^N ligands (L1-L5) are different diimine ligands that differ from each other in the number of aromatic rings fused to the bipyridine scaffold. The photophysical properties of IrL1-IrL5 were thoroughly studied, and theoretical calculations were performed for a deeper comprehension of the respective variations along the series. All complexes exhibited high photostability under blue light irradiation. An increase in the number of aromatic rings led to a reduction in the HOMO-LUMO band gap causing a red-shift in the absorbance bands. Although all the complexes generated singlet oxygen (1 O2 ) in aerated aqueous solutions through a photocatalytic process, IrL5 was by far the most efficient photosensitizer. Consequently, IrL5 was highly active in the photocatalytic oxidation of NADH. The formation of aggregates in DMSO at a high concentration (25 mM) was confirmed using different techniques, but was proved to be negligible in the concentration range of biological experiments. Moreover, ICP-MS studies proved that the cellular uptake of IrL2 and IrL3 is much better relative to that of IrL1, IrL4 and IrL5. The antiproliferative activity of IrL1-IrL5 was investigated in the dark and under blue light irradiation against different cancer cell lines. Complexes IrL1-IrL4 were found to be cytotoxic under dark conditions, while IrL5 turned out to be weakly cytotoxic. Despite the low cellular uptake of IrL5, this derivative exhibited a high increase of cytotoxicity upon blue light irradiation resulting in photocytotoxicity indexes (PI) up to 38. IrL1-IrL4 showed lower photocytotoxicity indexes ranging from 1.3 to 17.0. Haemolytic experiments corroborated the compatibility of our complexes with red blood cells. Confocal microscopy studies proved their accumulation in mitochondria, leading to mitochondrial membrane depolarization, and ruled out their localization in lysosomes. Overall, the mitochondria-targeted activity of IrL5, which inhibits considerably the viability of cancer cells upon blue light irradiation, allows us to outline this PS as a new alternative to traditional chemotherapeutic agents.- Published
- 2024
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33. Phototoxicity of cyclometallated Ir(III) complexes bearing a thio-bis-benzimidazole ligand, and its monodentate analogue, as potential PDT photosensitisers in cancer cell killing.
- Author
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Martínez-Alonso M, Jones CG, Shipp JD, Chekulaev D, Bryant HE, and Weinstein JA
- Subjects
- Ligands, Cell Line, Tumor, Photosensitizing Agents chemistry, Cell Death, Iridium pharmacology, Iridium chemistry, Photochemotherapy, Neoplasms, Benzimidazoles
- Abstract
Two novel cyclometallated iridium(III) complexes have been prepared with one bidentate or two monodentate imidazole-based ligands, 1 and 2, respectively. The complexes showed intense emission with long lifetimes of the excited state. Femtosecond transient absorption experiments established the nature of the lowest excited state as
3 IL state. Singlet oxygen generation with good yields (40% for 1 and 82% for 2) was established by detecting1 O2 directly, through its emission at 1270 nm. Photostability studies were also performed to assess the viability of the complexes as photosensitizers (PS) for photodynamic therapy (PDT). Complex 1 was selected as a good candidate to investigate light-activated killing of cells, whilst complex 2 was found to be toxic in the dark and unstable under light. Complex 1 demonstrated high phototoxicity indexes (PI) in the visible region, PI > 250 after irradiation at 405 nm and PI > 150 at 455 nm, in EJ bladder cancer cells., (© 2024. The Author(s).)- Published
- 2024
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34. A Novel Near-IR Absorbing Ruthenium(II) Complex as Photosensitizer for Photodynamic Therapy and its Cetuximab Bioconjugates.
- Author
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Martínez-Alonso M, Gandioso A, Thibaudeau C, Qin X, Arnoux P, Demeubayeva N, Guérineau V, Frochot C, Jung AC, Gaiddon C, and Gasser G
- Subjects
- Photosensitizing Agents pharmacology, Photosensitizing Agents chemistry, Cetuximab pharmacology, Ruthenium chemistry, Coordination Complexes chemistry, Photochemotherapy
- Abstract
A novel Ru(II) cyclometalated photosensitizer (PS), Ru-NH
2 , for photodynamic therapy (PDT) of formula [Ru(appy)(bphen)2 ]PF6 (where appy=4-amino-2-phenylpyridine and bphen=bathophenanthroline) and its cetuximab (CTX) bioconjugates, Ru-Mal-CTX and Ru-BAA-CTX (where Mal=maleimide and BAA=benzoylacrylic acid) were synthesised and characterised. The photophysical properties of Ru-NH2 revealed absorption maxima around 580 nm with an absorption up to 725 nm. The generation of singlet oxygen (1 O2 ) upon light irradiation was confirmed with a1 O2 quantum yield of 0.19 in acetonitrile. Preliminary in vitro experiments revealed the Ru-NH2 was nontoxic in the dark in CT-26 and SQ20B cell lines but showed outstanding phototoxicity when irradiated, reaching interesting phototoxicity indexes (PI) >370 at 670 nm, and >150 at 740 nm for CT-26 cells and >50 with NIR light in SQ20B cells. The antibody CTX was successfully attached to the complexes in view of the selective delivery of the PS to cancer cells. Up to four ruthenium fragments were anchored to the antibody (Ab), as confirmed by MALDI-TOF mass spectrometry. Nonetheless, the bioconjugates were not as photoactive as the Ru-NH2 complex., (© 2023 The Authors. ChemBioChem published by Wiley-VCH GmbH.)- Published
- 2023
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35. Impact of the "Zero Resistance" program on acquisition of multidrug-resistant bacteria in patients admitted to Intensive Care Units in Spain. A prospective, intervention, multimodal, multicenter study.
- Author
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Álvarez-Lerma F, Catalán-González M, Álvarez J, Sánchez-García M, Palomar-Martínez M, Fernández-Moreno I, Garnacho-Montero J, Barcenilla-Gaite F, García R, Aranaz-Andrés J, Lozano-García FJ, Ramírez-Galleymore P, and Martínez-Alonso M
- Subjects
- Humans, Spain epidemiology, Prospective Studies, Bacteria, Intensive Care Units, Hospitalization
- Abstract
Objective: To assess the impact of a multimodal interventional project ("Zero Resistance") on the acquisition of multidrug-resistant bacteria (MDR-B) during the patient's ICU stay., Design: Prospective, open-label, interventional, multicenter study., Setting: 103 ICUs., Patients: Critically ill patients admitted to the ICUs over a 27-month period., Interventions: Implementation of a bundle of 10 recommendations to prevent emergence and spread of MDR-B in the ICU., Main Variable of Interest: Rate of patients acquiring MDR-B during their ICU stay, with differentiation between colonization and infection., Results: A total of 139,505 patients were included. In 5409 (3.9%) patients, 6020 MDR-B on ICU admission were identified, and in 3648 (2.6%) patients, 4269 new MDR-B during ICU stay were isolated. The rate of patients with MDR-B detected on admission increased significantly (IRR 1.43, 95% CI 1.31-1.56) (p<0.001) during the study period, with an increase of 32.2% between the initial and final monthly rates. On the contrary, the rate of patients with MDR-B during ICU stay decreased non-significantly (IRR 0.93, 95% CI 0.83-1.03) (p=0.174), with a 24.9% decrease between initial and final monthly rates. According to the classification into colonization or infection, there was a highly significant increase of MDR-B colonizations detected on admission (IRR 1.69, 95% CI 1.52-1.83; p<0.0001) and a very significant decrease of MDR-B-infections during ICU stay (IRR 0.67, 95% CI 0.57-0.80, p<0.0001)., Conclusions: The implementation of ZR project-recommendations was associated with a significantly reduction an infection produced by MDR-B acquired during the patient's ICU stay., (Copyright © 2022. Published by Elsevier España, S.L.U.)
- Published
- 2023
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36. Platelet levels and age are determinants of survival after mild-moderate TBI: A prospective study in Spain.
- Author
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Yuguero O, Vena A, Bernal M, Martínez-Alonso M, Farre J, and Purroy F
- Subjects
- Aged, 80 and over, Female, Humans, Male, Prospective Studies, Spain epidemiology, Platelet Count, Age Factors, Survival Analysis, Injury Severity Score, Aged, Brain Injuries, Traumatic epidemiology, Brain Injuries, Traumatic therapy
- Abstract
Introduction: Traumatic brain injury (TBI) is a very important reason for consultation in emergency departments., Methods: A hospital cohort study with patients who attended a hospital emergency department between June 1, 2018 and December 31, 2020 due to TBI was studied. Clinical and sociodemographic variables were recorded. The levels of biomarkers and management variables were used. Qualitative variables were analyzed using Pearson's chi-square test, and quantitative variables using the Mann-Whitney U -test. Survival analyses were performed by fitting a multivariable Cox regression model for patient survival during the follow-up of the study in relation to the patient's characteristics upon admission to the emergency department., Results: A total of 540 patients were included. The mean age was 83 years, and 53.9% of the patients were men. Overall, 112 patients (20.7%) died during the study follow-up. The mortality rate per 100 person-years was 14.33 (11.8-17.24), the most frequent mechanism being falls in the home, with none caused on public roads. The multivariable Cox proportional hazards model showed that survival after TBI was significantly associated with age, S100 levels, Charlson index, patient's institutionalized status, the place where the TBI occurred, and hemoglobin and platelet levels., Discussion: The most common profile for a patient with a TBI was male and aged between 80 and 90 years. The combination of the variables age, Charlson index, place of TBI occurrence, and hemoglobin and platelet levels could offer early prediction of survival in our population independently of TBI severity. With the data obtained, a therapeutic algorithm could be established for patients suffering from mild TBI, allowing the patient to be supervised at home, avoiding futile referrals to emergency services., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Yuguero, Vena, Bernal, Martínez-Alonso, Farre and Purroy.)
- Published
- 2023
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37. Fungal treatment of agricultural washing wastewater: Comparison between two operational strategies.
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Beltrán-Flores E, Pla-Ferriol M, Martínez-Alonso M, Gaju N, Sarrà M, and Blánquez P
- Subjects
- Agriculture, Bioreactors, Biodegradation, Environmental, Wastewater, Pesticides
- Abstract
Agricultural washing wastewater (AWW) is an important source of pesticides that, given its intrinsic characteristics, has a high potential to be treated by fungal bioremediation using white rot fungi. In the present study, two AWW treatment strategies were compared: a fluidized-bed reactor (FBR) with T. versicolor pellets and a rotating drum bioreactor (RDB) with T. versicolor immobilized on wood. The RDB effluent showed better results in all studied parameters compared to those of the FBR, including pesticide removal (87%), toxicity, laccase activity, COD, absorbance and microbial communities. Additionally, the fungal assemblage showed that T. versicolor was successfully immobilized in the RDB, which triggered a major shift in the initial community. Afterwards, solid by-products were treated in a fungal biopile-like system reaching high biodegradation rates. Therefore, this study validates the fungal RDB as a viable alternative for AWW treatment, opening up the possibility of a further in-situ and full-scale application., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2023
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38. Targets, Mechanisms and Cytotoxicity of Half-Sandwich Ir(III) Complexes Are Modulated by Structural Modifications on the Benzazole Ancillary Ligand.
- Author
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Acuña MI, Rubio AR, Martínez-Alonso M, Busto N, Rodríguez AM, Davila-Ferreira N, Smythe C, Espino G, García B, and Domínguez F
- Abstract
Cancers are driven by multiple genetic mutations but evolve to evade treatments targeting specific mutations. Nonetheless, cancers cannot evade a treatment that targets mitochondria, which are essential for tumor progression. Iridium complexes have shown anticancer properties, but they lack specificity for their intracellular targets, leading to undesirable side effects. Herein we present a systematic study on structure-activity relationships of eight arylbenzazole-based Iridium(III) complexes of type [IrCl(Cp*)], that have revealed the role of each atom of the ancillary ligand in the physical chemistry properties, cytotoxicity and mechanism of biological action. Neutral complexes, especially those bearing phenylbenzimidazole (HL1 and HL2), restrict the binding to DNA and albumin. One of them, complex 1[C,NH-Cl] , is the most selective one, does not bind DNA, targets exclusively the mitochondria, disturbs the mitochondria membrane permeability inducing proton leak and increases ROS levels, triggering the molecular machinery of regulated cell death. In mice with orthotopic lung tumors, the administration of complex 1[C,NH-Cl] reduced the tumor burden. Cancers are more vulnerable than normal tissues to a treatment that harnesses mitochondrial dysfunction. Thus, complex 1[C,NH-Cl] characterization opens the way to the development of new compounds to exploit this vulnerability.
- Published
- 2022
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39. Correction: Martinez-Gonzalez et al. Adaptation and Validation of the Diabetic Foot Ulcer Scale-Short Form in Spanish Subjects. J. Clin. Med. 2020, 9 , 2497.
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Martinez-Gonzalez D, Dòria M, Martínez-Alonso M, Alcubierre N, Valls J, Verdú-Soriano J, Granado-Casas M, and Mauricio D
- Abstract
In the original publication [...].
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- 2022
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40. Fungal bioremediation of agricultural wastewater in a long-term treatment: biomass stabilization by immobilization strategy.
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Beltrán-Flores E, Pla-Ferriol M, Martínez-Alonso M, Gaju N, Blánquez P, and Sarrà M
- Subjects
- Biodegradation, Environmental, Biomass, Trametes metabolism, Pesticides metabolism, Wastewater microbiology
- Abstract
Fungal bioremediation emerges as an effective technology for pesticide treatment, but its successful implementation depends on overcoming the problem of microbial contamination. In this regard, fungal immobilization on wood seems to be a promising strategy, but there are two main drawbacks: the predominant removal of pesticides by sorption and fungal detachment. In this study, agricultural wastewater with pesticides was treated by Trametes versicolor immobilized on wood chips in a rotary drum bioreactor (RDB) for 225 days, achieving fungal consolidation and high pesticide biodegradation through two main improvements: the use of a more favorable substrate and the modification of operating conditions. Fungal community dynamic was assessed by denaturing gradient gel electrophoresis (DGGE) analysis and subsequent prominent band sequencing, showing a quite stable community in the RDB, mainly attributed to the presence of T. versicolor. Pesticide removals were up to 54 % diuron and 48 % bentazon throughout the treatment. Afterwards, pesticide-contaminated wood chips were treated by T. versicolor in a solid biopile-like system. Hence, these results demonstrate that the microbial contamination constraint has definitely been overcome, and fungal bioremediation technology is ready to be implemented on a larger scale., Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Eduardo Beltran Flores reports financial support was provided by Spanish Ministry of Economy and Competitiveness State Research Agency., (Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2022
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41. Feasibility and Acceptability of Personalized Breast Cancer Screening (DECIDO Study): A Single-Arm Proof-of-Concept Trial.
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Laza-Vásquez C, Martínez-Alonso M, Forné-Izquierdo C, Vilaplana-Mayoral J, Cruz-Esteve I, Sánchez-López I, Reñé-Reñé M, Cazorla-Sánchez C, Hernández-Andreu M, Galindo-Ortego G, Llorens-Gabandé M, Pons-Rodríguez A, and Rué M
- Subjects
- Feasibility Studies, Female, Humans, Mammography, Mass Screening, Proof of Concept Study, Breast Neoplasms diagnosis, Breast Neoplasms genetics, Early Detection of Cancer
- Abstract
The aim of this study was to assess the acceptability and feasibility of offering risk-based breast cancer screening and its integration into regular clinical practice. A single-arm proof-of-concept trial was conducted with a sample of 387 women aged 40-50 years residing in the city of Lleida (Spain). The study intervention consisted of breast cancer risk estimation, risk communication and screening recommendations, and a follow-up. A polygenic risk score with 83 single nucleotide polymorphisms was used to update the Breast Cancer Surveillance Consortium risk model and estimate the 5-year absolute risk of breast cancer. The women expressed a positive attitude towards varying the frequency of breast screening according to individual risk and, especially, more frequently inviting women at higher-than-average risk. A lower intensity screening for women at lower risk was not as welcome, although half of the participants would accept it. Knowledge of the benefits and harms of breast screening was low, especially with regard to false positives and overdiagnosis. The women expressed a high understanding of individual risk and screening recommendations. The participants' intention to participate in risk-based screening and satisfaction at 1-year were very high.
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- 2022
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42. A Simple Scoring System to Differentiate Bacterial from Viral Infections in Acute Exacerbations of COPD Requiring Hospitalization.
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Ruiz-González A, Sáez-Huerta E, Martínez-Alonso M, Bernet-Sánchez A, and Porcel JM
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- Anti-Bacterial Agents therapeutic use, Bacteria, Hospitalization, Humans, Bacterial Infections diagnosis, Bacterial Infections drug therapy, Pulmonary Disease, Chronic Obstructive drug therapy, Respiratory Tract Infections microbiology, Virus Diseases diagnosis
- Abstract
Objective: Both bacteria and viruses may cause acute exacerbations of chronic obstructive pulmonary disease (AECOPD). The objective of this study was to identify readily available clinical parameters to discriminate between them., Methods: During a winter period all consecutive patients with an AECOP who were hospitalized in a non-ICU general ward were prospectively enrolled. In addition to blood tests, cultures of spontaneous or induced sputum samples, and genome detection of respiratory viruses in nasopharyngeal swab samples using multiplex RT-PCR assays were obtained. Only patients with positive microbiological results (bacteria, virus, or both) were eventually included. Mixed infections (bacteria plus viruses) were categorized into the bacterial group due to therapeutic implications (ie, need for antibiotics). Demographic and routine clinical and analytical information was collected., Results: A total of 127 AECOPD patients out of 213 initially evaluated met inclusion criteria and were classified as having bacterial (70, 55.1%) or viral (57, 44.9%) infection. Although no single variable was useful to identify bacteria, the combination of serum C-reactive protein >70 mg/L (2 points), >1 day of symptoms (1.5 points), and a blood neutrophil count >9,500 x10
9 /L (1 point) into a scoring system reached an AUC of 0.80 (95% CI=0.73-0.88) for bacterial etiologies. With this model, scoring 0 or 1 point significantly reduced the probability of a bacterial infection (likelihood ratio negative of 0.2), whereas summing up 2.5 points or more increased it sufficiently to be clinically meaningful (likelihood ratio positive >3.7). Viral infections resulted in fewer hospitalization days (78.9% of patients spent ≥3 days in hospital vs 95.7% of those with bacterial infections; P =0.008)., Conclusion: A simple and easy to obtain score system can help clinicians in the decision of prescribing antibiotics in AECOPD patients., Competing Interests: The authors report no conflicts of interest in this work., (© 2022 Ruiz-González et al.)- Published
- 2022
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43. Subclinical atheromatosis localization and burden in a low-to-moderate cardiovascular risk population: the ILERVAS study.
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Bermúdez-López M, Martínez-Alonso M, Castro-Boqué E, Betriu À, Cambray S, Farràs C, Barbé F, Pamplona R, Lecube A, Mauricio D, Purroy F, Valdivielso JM, and Fernández E
- Subjects
- Aged, Female, Heart Disease Risk Factors, Humans, Male, Middle Aged, Risk Factors, Atherosclerosis diagnosis, Atherosclerosis epidemiology, Cardiovascular Diseases epidemiology, Plaque, Atherosclerotic diagnostic imaging, Plaque, Atherosclerotic epidemiology
- Abstract
Introduction and Objectives: There is a discrepancy between risk assessment based on cardiovascular risk factors (CVRF) and atheromatosis burden. The objective was to identify the prevalence of subclinical diseases with common risk factors, such as atheromatosis, occult kidney disease, prediabetes, and diabetes in a middle-aged population with low-to-moderate cardiovascular risk; to assess the vascular distribution, and severity of subclinical atheromatosis., Methods: Randomized, interventional, longitudinal clinical trial. The intervention consisted of vascular ultrasound examination in the carotid and femoral arteries assessing 12 territories, combined with clinical, anthropometric, lifestyle, and biochemical parameters. Inclusion criteria consisted of women (aged 50-70 years) and men (aged 45-65 years) with at least 1 CVRF. Exclusion criteria consisted of a clinical history of diabetes, chronic kidney disease, or a prior CV event. Here, baseline characteristics of the ILERVAS cohort are shown., Results: A total of 8330 middle-aged asymptomatic participants, 50.7% women, were enrolled. The presence of 1-2 CVRF was found in 74.8% and adherence to the Mediterranean diet was low in 52.8%. Several previously unknown chronic diseases were diagnosed, such as dyslipidemia (21.1%), hypertension (15.3%), kidney disease (15.4%), obesity (10.6%), and diabetes (2.3%). Subclinical atheromatosis was found in 71.4% of participants, localized in common femoral (54.5%), carotid bifurcation (41.1%) and internal carotid (22%). Intermediate atheromatosis (2-3 territories with atheroma plaque) was found in 32.6%, and generalized atheromatosis (>3 territories) in 19.7. Total plaque area was higher in men (0.97 cm
2 vs 0.58 cm2 , P<.001). Total plaque area was also higher in the femoral artery, and increased with the number of CVRF., Conclusions: Subclinical atheromatosis was highly prevalent in a middle-aged population with low-to moderate cardiovascular risk, with 1 in 5 participants having generalized atheromatosis. ClinicalTrials.gov Identifier: NCT03228459., (Copyright © 2020 Sociedad Española de Cardiología. Published by Elsevier España, S.L.U. All rights reserved.)- Published
- 2021
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44. Psychometric Validation of the Cardiff Wound Impact Schedule Questionnaire in a Spanish Population with Diabetic Foot Ulcer.
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Granado-Casas M, Martinez-Gonzalez D, Martínez-Alonso M, Dòria M, Alcubierre N, Valls J, Julve J, Verdú-Soriano J, and Mauricio D
- Abstract
Diabetic foot ulcers (DFU) negatively affect the quality of life (QoL) of people with diabetes. The Cardiff Wound Impact Schedule (CWIS) questionnaire has been designed to measure the QoL of people with chronic foot wounds. However, no studies have been specifically designed to validate this instrument in a Spanish population. In this prospective study, a total of 141 subjects with DFU were recruited. DFU was determined by performing physical examinations. Medical records were exhaustively reviewed to collect clinical variables. The CWIS was transculturally adapted by a group of experts and a group of patients with DFU. The SF-36 and EQ-5D generic instruments were used as reference tools. The questionnaires were administered at 7 days and 4, 12, and 26 weeks after the baseline assessment by personal interview with each of the study subjects. The psychometric properties of the instrument were assessed using statistical methods. The content validity had an average of 3.63 (90.7% of the maximum score of 4). The internal consistency of the CWIS subscales had a standardized Cronbach's alpha range from 0.715 to 0.797. The reproducibility was moderate with an intraclass correlation coefficient (ICC) range from 0.606 to 0.868. Significant correlations between CWIS domains and SF-36 and EQ-5D subscales were observed, demonstrating a good criterion validity of the CWIS questionnaire ( p < 0.001). However, the construct validity of the CWIS was not validated with a comparative fit index (CFI) of 0.69, a root mean square error of approximation (RMSEA) of 0.09, and a standardized root mean square residual (SRMR) of 0.10. The sensitivity to changes over time was optimal in the three domains (i.e., social life, well-being, and physical symptoms) ( p < 0.001). In conclusion, the Spanish version of the CWIS shows acceptable psychometric properties to assess the QoL of subjects with DFU, except for its construct validity.
- Published
- 2021
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45. [Informed choice in breast cancer screening: the role of education].
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Pons-Rodríguez A, Martínez-Alonso M, Perestelo-Pérez L, Garcia M, Sala M, and Rué M
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- Choice Behavior, Decision Making, Decision Support Techniques, Early Detection of Cancer, Female, Health Knowledge, Attitudes, Practice, Humans, Mammography, Mass Screening, Breast Neoplasms diagnosis
- Abstract
Objective: To evaluate the effect of receiving information about the benefits and harms of breast cancer screening in informed choice, according to educational level., Method: Secondary analysis of a randomized, controlled study, in four screening programs, in Catalonia and the Canary Islands (Spain). We analyzed 400 women who were going to be invited to participate for the first time. The intervention group received a decision aid that showed the benefits and harms of screening. The control group received a standard brochure that recommended participating in the screening program. Educational level was grouped into two categories, low and high. The primary outcome was informed choice defined as adequate knowledge and consistency between attitudes and intentions., Results: The intervention produced a greater increase in knowledge in women with a high educational level compared to those with a lower educational level. Among women who received the intervention, informed choice was almost three times higher in those with a high educational level (27% versus 11%). No differences were observed between educational levels in decisional conflict, confidence in the decision, anxiety and worry about breast cancer, in the intervention and control groups., Conclusions: A decision aid for breast cancer screening had much more impact on informed choice among women with a high educational level. In women with low educational level, the attitude towards screening improved and there was an increase in the intention to be screened., (Copyright © 2020 SESPAS. Publicado por Elsevier España, S.L.U. All rights reserved.)
- Published
- 2021
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46. Clinical Usefulness of Anthropometric Indices to Predict the Presence of Prediabetes. Data from the ILERVAS Cohort.
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Sánchez M, Sánchez E, Bermúdez-López M, Torres G, Farràs-Sallés C, Pamplona R, Castro-Boqué E, Valdivielso JM, Purroy F, Martínez-Alonso M, Godoy P, Mauricio D, Fernández E, Hernández M, Rius F, Lecube A, and On Behalf Of The Ilervas Project Collaborators
- Subjects
- Adiposity, Biomarkers, Body Weight, Cohort Studies, Cross-Sectional Studies, Diabetes Mellitus, Type 2, Female, Glycated Hemoglobin, Humans, Male, Obesity, Obesity, Abdominal, Overweight, ROC Curve, Anthropometry methods, Prediabetic State diagnosis
- Abstract
Prediabetes is closely related to excess body weight and adipose distribution. For this reason, we aimed to assess and compare the diagnostic usefulness of ten anthropometric adiposity indices to predict prediabetes. Cross-sectional study with 8188 overweight subjects free of type 2 diabetes from the ILERVAS project (NCT03228459). Prediabetes was diagnosed by levels of glycated hemoglobin (HbA1c). Total body adiposity indices [BMI, Clínica Universidad de Navarra-Body Adiposity Estimator (CUN-BAE) and Deurenberg's formula] and abdominal adiposity (waist and neck circumferences, conicity index, waist to height ratio, Bonora's equation, A body shape index, and body roundness index) were calculated. The area under the receiver-operating characteristic (ROC) curve, the best cutoff and the prevalence of prediabetes around this value were calculated for every anthropometric index. All anthropometric indices other than the A body adiposity were higher in men and women with prediabetes compared with controls ( p < 0.001 for all). In addition, a slightly positive correlation was found between indices and HbA1c in both sexes (r ≤ 0.182 and p ≤ 0.026 for all). None of the measures achieved acceptable levels of discrimination in ROC analysis (area under the ROC ≤ 0.63 for all). Assessing BMI, the prevalence of prediabetes among men increased from 20.4% to 36.2% around the cutoff of 28.2 kg/m
2 , with similar data among women (from 29.3 to 44.8% with a cutoff of 28.6 kg/m2 ). No lonely obesity index appears to be the perfect biomarker to use in clinical practice to detect individuals with prediabetes.- Published
- 2021
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47. Feasibility and acceptability of personalised breast cancer screening (DECIDO study): protocol of a single-arm proof-of-concept trial.
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Pons-Rodriguez A, Forné Izquierdo C, Vilaplana-Mayoral J, Cruz-Esteve I, Sánchez-López I, Reñé-Reñé M, Cazorla C, Hernández-Andreu M, Galindo-Ortego G, Llorens Gabandé M, Laza-Vásquez C, Balaguer-Llaquet P, Martínez-Alonso M, and Rué M
- Subjects
- Adult, Bayes Theorem, Feasibility Studies, Female, Humans, Middle Aged, Spain, Breast Neoplasms diagnosis, Breast Neoplasms genetics, Early Detection of Cancer
- Abstract
Introduction: Personalised cancer screening aims to improve benefits, reduce harms and being more cost-effective than age-based screening. The objective of the DECIDO study is to assess the acceptability and feasibility of offering risk-based personalised breast cancer screening and its integration in regular clinical practice in a National Health System setting., Methods and Analysis: The study is designed as a single-arm proof-of-concept trial. The study sample will include 385 women aged 40-50 years resident in a primary care health area in Spain. The study intervention consists of (1) a baseline visit; (2) breast cancer risk estimation; (3) a second visit for risk communication and screening recommendations based on breast cancer risk and (4) a follow-up to obtain the study outcomes.A polygenic risk score (PRS) will be constructed as a composite likelihood ratio of 83 single nucleotide polymorphisms. The Breast Cancer Surveillance Consortium risk model, including age, race/ethnicity, family history of breast cancer, benign breast disease and breast density will be used to estimate a preliminary 5-year absolute risk of breast cancer. A Bayesian approach will be used to update this risk with the PRS value.The primary outcome measures will be attitude towards, intention to participate in and satisfaction with personalised breast cancer screening. Secondary outcomes will include the proportions of women who accept to participate and who complete the different phases of the study. The exact binomial and the Student's t-test will be used to obtain 95% CIs., Ethics and Dissemination: The study protocol was approved by the Drug Research Ethics Committee of the University Hospital Arnau de Vilanova. The trial will be conducted in compliance with this study protocol, the Declaration of Helsinki and Good Clinical Practice.The results will be published in peer-reviewed scientific journals and disseminated in scientific conferences and media., Trial Registration Number: NCT03791008., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)
- Published
- 2020
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48. Experimental and theoretical characterization of the strong effects on DNA stability caused by half-sandwich Ru(II) and Ir(III) bearing thiabendazole complexes.
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Santolaya J, Busto N, Martínez-Alonso M, Espino G, Grunenberg J, Barone G, and García B
- Subjects
- Coordination Complexes metabolism, DNA metabolism, Nucleic Acid Conformation, Coordination Complexes chemistry, Coordination Complexes pharmacology, DNA chemistry, Iridium chemistry, Molecular Dynamics Simulation, Ruthenium chemistry, Thiabendazole chemistry
- Abstract
The synthesis and characterization of two half-sandwich complexes of Ru(II) and Ir(III) with thiabendazole as ancillary ligand and their DNA binding ability were investigated using experimental and computational methods.
1 H NMR and acid-base studies have shown that aquo-complexes are the reactive species. Kinetic studies show that both complexes bind covalently to DNA through the metal site and non covalently through the ancillary ligand. Thermal stability studies, viscosity, circular dichroism measurements and quantum chemical calculations have shown that the covalent binding causes breaking of the H-bonding between base pairs, bringing about DNA denaturation and compaction. Additionally, molecular dynamics (MD) simulations and quantum mechanics/molecular mechanics (QM/MM) calculations shed light into the binding features of the Ru(II) and Ir(III) complexes and their respective enantiomers toward double-helical DNA, highlighting the important role played by the NˆN ancillary ligand once the complexes are covalently linked to DNA. Moreover, metal quantification in the nucleus of SW480 colon adenocarcinoma cells were carried out by inductively coupled plasma-mass spectrometry (ICP-MS), both complexes are more internalized than cisplatin after 4 h of exposition. However, in spite of the dramatic changes in the helicity of the DNA secondary structure induced by these complexes and their nuclear localization, antiproliferative studies have revealed that both, Ru(II) and Ir(III) complexes, cannot be considered cytotoxic. This unexpected behavior can be justified by the fast formation of aquo-complexes, which may react with components of the cell culture medium or the cytoplasm compartment in such a way that they may become deactivated before reaching DNA.- Published
- 2020
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49. Adaptation and Validation of the Diabetic Foot Ulcer Scale-Short Form in Spanish Subjects.
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Martinez-Gonzalez D, Dòria M, Martínez-Alonso M, Alcubierre N, Valls J, Verdú-Soriano J, Granado-Casas M, and Mauricio D
- Abstract
Diabetic foot ulcer (DFU) is a chronic complication that negatively affects the quality of life (QoL) of diabetic patients. In Spain, there is no specifically designed and validated instrument to assess the QoL of patients with DFU. Our aim was to adapt the Diabetic Foot Ulcer Scale-Short Form (DFS-SF) questionnaire to a Spanish population and validate it. A prospective, observational design was used. The DFS-SF was administered by personal interview. The validated SF-36 and EQ-5D generic instruments were used as reference tools. The reliability, validity, and sensitivity to changes were assessed using standard statistical methods. A sample of 141 patients with DFU was recruited. The content validity was 3.46 on average (maximum score of 4). The internal consistency of the DFS-SF subscales showed a standardized Cronbach's α range between 0.720 and 0.948. The DFS-SF domains showed excellent reproducibility measures (intraclass correlation coefficient from 0.77-0.92). The criterion validity was good with significant correlations between each DFS-SF subscale and its corresponding SF-36 and EQ-5D subscales ( p < 0.001). However, the questionnaire structure was not validated (comparative fit index = 0.844, root mean square error of approximation = 0.095, and standardized root mean square residual = 0.093). The instrument showed high sensitivity to ulcer changes over time ( p < 0.001). The adapted and validated Spanish version of the DFS-SF questionnaire has good psychometric properties and shows good sensitivity to ulcer changes, although the construct validity was not optimal. The adapted questionnaire will be a useful tool specifically to assess the QoL in subjects with diabetic foot ulcers in the clinical and research settings in Spain.
- Published
- 2020
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50. The Mediterranean Diet is Associated with an Improved Quality of Life in Adults with Type 1 Diabetes.
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Granado-Casas M, Martin M, Martínez-Alonso M, Alcubierre N, Hernández M, Alonso N, Castelblanco E, and Mauricio D
- Subjects
- Adult, Cross-Sectional Studies, Diet, Healthy, Female, Humans, Male, Middle Aged, Surveys and Questionnaires, Diabetes Mellitus, Type 1 diet therapy, Diet, Mediterranean, Quality of Life
- Abstract
This study aimed to assess the potential association between dietary patterns (i.e., the Mediterranean Diet (MedDiet) and healthy eating) and patient-reported quality of life (QoL) and treatment satisfaction (TS) in adults with type 1 diabetes (T1D). A food frequency questionnaire, the Audit of Diabetes-Dependent Quality of Life (ADDQoL-19), and the Diabetes Treatment Satisfaction Questionnaire-status version (DTSQ-s) were administered via personal interviews to 258 participants with T1D. Multivariable analysis showed that a moderate or high adherence to the MedDiet was associated with greater diabetes-specific QoL (β = 0.32, 95% CI = 0.03; 0.61; p = 0.029). None of the dietary quality indexes (i.e., the alternate Mediterranean Diet Score (aMED) and the alternate Healthy Eating Index (aHEI)) were associated with the overall TS. However, the aHEI was positively associated with the specific items of TS "convenience" and "flexibility" (β = 0.03, 95% CI = 0.00; 0.06; p = 0.042 and β = 0.04; 95% CI = 0.01; 0.06; p = 0.011, respectively). On the other hand, the aHEI was negatively associated with the dimension "recommend to others" (β = -0.5, 95% CI = -0.99; -0.02; p = 0.042). In conclusion, a moderate and high adherence to the MedDiet was associated with greater QoL. Although neither aMED nor aHEI were associated with the overall TS, some specific items were positively (i.e., "convenience", "flexibility") or negatively ("recommend to others") related to the aHEI. Further research is needed to assess how to improve medical nutrition therapy and its impact on patient-reported outcomes in people with T1D.
- Published
- 2020
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