1. Human Chorionic Gonadotropin Treatment of Follicular Cysts in Female Guinea Pigs (Cavia Porcellus)
- Author
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Secher, Jan Bojsen-Møller, Müller, Anna Vilhelmina, Rueløkke, Mette Lybek, Vildershøj, Christina, Secher, Jan Bojsen-Møller, Müller, Anna Vilhelmina, Rueløkke, Mette Lybek, and Vildershøj, Christina
- Abstract
Bilateral non-pruritisk alopeci hos hunmarsvin er ofte associeret med hyperøstrogenisme forårsaget af follikelcyster, og behandling er sædvanligvis ovariehysterektomi (OHE). Medicinsk behandling med hormonet humant choriongonadotropin (hCG) er et alternativ, dog er forskning på området begrænset. Det primære formål med dette studie var at undersøge hvor effektivt og sikkert behandling med hCG er, og sammenligne det med OHE. Dette blev gjort vha. et klinisk forsøg, hvor 7 kæledyrs hunmarsvin blev behandlet med 100 IE/kg hCG subkutant (SC) én gang ugentligt i 3 uger og de kliniske tegn blev herefter monitoreret i 8 uger. En historisk kontrolgruppe bestod af 8 tilfælde af hunmarsvin med bilateral non-pruritisk alopeci, som kirurgisk havde fået fjernet ovarierne ved OHE eller ovariektomi inden dette studie. Alle patienter i begge grupper havde resolution af de kliniske tegn og der var derfor ingen forskel i responset på behandlingen mellem de to grupper. Risikoen ved behandlingen med hCG blev fundet at være relativt lille med kun et enkelt tilfælde af mild reaktion ved injektionsstedet efter tredje injektion. Studiet undersøgte også frekvensen af andre kliniske tegn associeret med follikelcyster og hyper-østrogenisme og hvor hurtigt man kunne se resolution efter behandlingen. Adfærdsændringer (71%) og hyperkeratose om papillae mammae (57%) var de hyppigste tegn, men også vægttab (29%) og fravær af vaginalmembranen (29%) blev observeret. Ny hårvækst kunne ses fra dag 8 og de fleste havde fuld resolution på dag 56. Hyperkeratose om papillae mammae begyndte at forsvinde fra dag 28 for de fleste, og en positiv adfærdsændring såvel som lukning af vaginalåbningen blev set på dag 8-15. Vægten steg støt for 5 ud af 7 patienter, til trods for at kun 2 havde anamnese om vægttab. Derudover blev der foretaget en seriel transabdominal ultralydsundersøgelse af ovarier og cyster for at afgøre, 1) om det var muligt at differentiere follikelcyster fra serøse cyster på en ultraly, Bilateral non-pruritic alopecia in the female guinea pig is often associated with hyperestrogenism caused by follicular cysts and treatment is usually ovariohysterectomy (OHE). Medical treatment with the hormone human chorionic gonadotropin (hCG) is an alternative, though knowledge within this area is lacking. The primary aim of this study was to examine the efficacy and safety of treatment with hCG and compare it to OHE. This was done by a clinical trial treating 7 female pet guinea pigs with 100 IU/kg hCG administered subcutaneously (SC) once a week for 3 weeks and monitoring clinical signs for 8 weeks. A historical control group was made of 8 female guinea pigs with bilateral non-pruritic alopecia having undergone OHE or ovariectomy prior to this study. All patients in both groups had clinical resolution and no difference in response to treatment was found between the groups. The risk of treatment with hCG was found to be relatively small with only one mild case of injection site reaction in relation to the third treatment. The study also examined the prevalence of other clinical signs associated with follicular cysts and hyperestrogenism and how quickly a resolution could be seen after treatment. Behavioral change (71%) and mammary hyperkeratosis (57%) were the most frequent, but weight loss (29%) and absence of the vaginal membrane (29%) were also observed. New hair growth was seen from day 8 and most had full resolution by day 56. Mammary hyperkeratosis started to improve by day 28 in most cases, and a positive behavioral change, as well as the closing of the vaginal opening, was observed on days 8-15. Weight increase was seen steadily for 5 of the 7 patients, even though only 2 had a history of weight loss. Additionally, a study of serial transabdominal ultrasound examination of the ovaries and cysts was performed to determine, 1) if it was possible to differentiate follicular cysts from serous cysts on ultrasound by their size and expected reaction
- Published
- 2024