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This paper presents comparative research on marine shell from four 19th century historical archaeological sites in Melbourne. The shell derives predominantly from Mud Oyster (Ostrea angasi) and Sydney Rock Oyster (Saccostrea glomerata) commercially harvested from natural reefs along the south‐east Australian coastline. The research collects quantitative data that informs on the 19th century oyster industry and investigates inter‐site shell variability and its implications for processing, consumption and discard. Dredging of subtidal reefs provides an explanation for the numerical dominance of oyster, the presence of subfossil cultch (Anadara) and the wide range of minor shellfish. Mud oyster and Sydney rock oyster comparisons in valve size, sided counts and preservation record significant differences within and between sites due to the origins, depositional conditions and the processing of the shell. These data form the basis of two models. The first predicts the archaeological representation of reef dredging and ranks shellfish according to categories, from live oysters to dead shell sampled from the reef substrate. Based on oyster shell anatomy and the separate uses of the right (lid) and left (dish) valves, the second model considers how oyster processing and consumption is characterised archaeologically in differential valve counts and pairing rates. [ABSTRACT FROM AUTHOR]

Chronic bone degenerative diseases represent a major threat to the health and well-being of the population, particularly those with advanced age. This study isolated exosomes (EXO), natural nano-particles, from dendritic cells, the “directors” of the immune response, to examine the immunobiology of DC EXO in mice, and their ability to reprogram immune cells responsible for experimental alveolar bone loss in vivo. Distinct DC EXO subtypes including immune-regulatory (regDC EXO), loaded with TGFB1 and IL10 after purification, along with immune stimulatory (stimDC EXO) and immune “null” immature (iDCs EXO) unmodified after purification, were delivered via I.V. route or locally into the soft tissues overlying the alveolar bone. Locally administrated regDC EXO showed high affinity for inflamed sites, and were taken up by both DCs and T cells in situ. RegDC EXO-encapsulated immunoregulatory cargo (TGFB1 and IL10) was protected from proteolytic degradation. Moreover, maturation of recipient DCs and induction of Th17 effectors was suppressed by regDC EXO, while T-regulatory cell recruitment was promoted, resulting in inhibition of bone resorptive cytokines and reduction in osteoclastic bone loss. This work is the first demonstration of DC exosome-based therapy for a degenerative alveolar bone disease and provides the basis for a novel treatment strategy.

Our previous studies have shown that systemic neonatal murine cytomegalovirus (MCMV) infection of BALB/c mice spread to the eye with subsequent establishment of latency in choroid/RPE. In this study, RNA sequencing (RNA-Seq) analysis was used to determine the molecular genetic changes and pathways affected by ocular MCMV latency. MCMV (50 pfu per mouse) or medium as control were injected intra-peritoneally (i.p.) into BALB/c mice at <3 days after birth. At 18 months post injection, the mice were euthanized, and the eyes were collected and prepared for RNA-Seq. Compared to three uninfected control eyes, we identified 321 differentially expressed genes (DEGs) in six infected eyes. Using the QIAGEN Ingenuity Pathway Analysis (QIAGEN IPA), we identified 17 affected canonical pathways, 10 of which function in neuroretinal signaling, with the majority of DEGs being downregulated, while 7 pathways function in upregulated immune/inflammatory responses. Retinal and epithelial cell death pathways involving both apoptosis and necroptosis were also activated. MCMV ocular latency is associated with upregulation of immune and inflammatory responses and downregulation of multiple neuroretinal signaling pathways. Cell death signaling pathways are also activated and contribute to the degeneration of photoreceptors, RPE, and choroidal capillaries. [ABSTRACT FROM AUTHOR]

Although from an athletic performance perspective it may be beneficial to undertake drop jump training when fatigued (principle of 'specificity' of training), such endurance fatigue may expose the body to a greater risk of injury if it causes an increase in peak impact accelerations. This study aimed to determine if endurance fatigue resulted in an increase in tibial peak impact acceleration and an associated change in knee kinematics when completing plyometric drop jumps. Fifteen females performed drop jumps from 3 heights (15, 30, and 45 cm) when fatigued and nonfatigued. Treadmill running was used to induce endurance fatigue. The following variables were assessed: tibial peak impact acceleration, knee angle at initial ground contact, maximum angle of flexion, range of flexion, and peak knee angular velocity. Fatigue resulted in significantly greater (p < 0.05) tibial peak impact acceleration and knee flexion peak angular velocity in drop jumps from 15 and 30 cm, but not from 45 cm. Fatigue had no effect on any of the knee angles assessed. The neuromuscular system was affected negatively by endurance fatigue at 15 and 30 cm, indicating that coaches should be aware of a potential increased risk of injury in performing drop jumps when fatigued. Because from the greater drop height of 45 cm the neuromuscular system had a reduced capacity to attenuate the impact accelerations per se, whether nonfatigued or fatigued, this would suggest that this height may have been too great for the athletes examined. KEY WORDS injury, plyometric, impact, landing

Fibro-adipogenic progenitor cells (FAPs) are a population of stem cells in skeletal muscle that play multiple roles in muscle repair and regeneration through their complex secretome; however, it is not well understood how the FAP secretome is altered with muscle disuse atrophy. Previous work suggests that the inflammatory cytokine IL-1β is increased in FAPs with disuse and denervation. Inflammasome activation and IL-1β secretion are also known to stimulate the release of extracellular vesicles (EVs). Here, we examined the microRNA (miRNA) cargo of FAP-derived, platelet-derived growth factor receptor A (PDGFRaþ) EVs from hindlimb muscles of wild-type and IL-1β KO mice after 14 days of single-hindlimb immobilization. Hindlimb muscles were isolated from mice following the immobilization period, and PDGFRaþ extracellular vesicles were isolated using size-exclusion chromatography and immunoprecipitation. Microarrays were performed to detect changes in miRNAs with unloading and IL-1β deficiency. Results indicate that the PDGFRaþ, FAP-derived EVs show a significant increase in miRNAs, such as miR-let-7c, miR-let-7b, miR-181a, and miR-124. These miRNAs have previously been demonstrated to play important roles in cellular senescence and muscle atrophy. Furthermore, the expression of these same miRNAs was not significantly altered in FAP-derived EVs isolated from the immobilized IL-1β KO. These data suggest that disuse-related activation of IL-1β can mediate the miRNA cargo of FAP-derived EVs, contributing directly to the release of senescence- and atrophyrelated miRNAs. Therapies targeting FAPs in settings associated with muscle disuse atrophy may therefore have the potential to preserve muscle function and enhance muscle recovery. [ABSTRACT FROM AUTHOR]

When reporting a subject's mean movement pattern, it is important to ensure that reported values are representative of the subject's typical movement. While previous studies have used the mean of 3 trials, scientific justification of this number is lacking. One approach is to determine statistically how many trials are required to achieve a representative mean. This study compared 4 methods of calculating the number of trials required in a hopping movement to achieve a representative mean. Fifteen males completed 15 trials of a lateral hurdle hop. Range of motion at the trunk, pelvis, hip, knee, and ankle, in addition to peak moments for the latter 3 joints were examined. The number of trials required was computed using a peak intraclass correlation coefficient method, sequential analysis with a bandwidth of acceptable variance in the mean, and a novel method based on the standard error of measurement (SEMind). The number of trials required across all variables ranged from 2 to 12 depending on method, joint, and anatomical plane. The authors advocate the SEMind method as it demonstrated fewer limitations than the other methods. Using the SEMind, the required number of trials for a representative mean during the lateral hurdle hop is 6. [ABSTRACT FROM AUTHOR]

Context: Chronic athletic groin pain (AGP) is common in field sports and has been associated with abnormal movement control and loading of the hip and pelvis during play. A single-leg squat (SLS) is commonly used by clinicians to assess movement control, but whether it can provide insight into control during more dynamic sporting movements in AGP patients is unclear. Objective: To determine the relationships between biomechanical measures in an SLS and the same measures in a single-leg drop landing, single-leg hurdle hop, and a cutting maneuver in AGP patients. Design: Cross-sectional study. Setting: Biomechanics laboratory. Patients: 40 recreational field-sports players diagnosed with AGP. Intervention: A biomechanical analysis of each individual's SLS, drop landing, hurdle hop, and cut was undertaken. Main Outcome Measures: Hip, knee, and pelvis angular displacement and hip and knee peak moments. Pearson product-moment correlations were used to examine relationships between SLS measures and equivalent measures in the other movements. Results: There were no significant correlations between any hip or pelvis measure in the SLS with the same measures in the drop landing, hurdle hop, or cut (r = .03-.43, P > .05). Knee frontal- and transverse-plane angular displacement were related in the SLS and drop landing only, while knee moments were related in the SLS, drop-landing, and hurdle hop (r = .50-.67, P < .05). Conclusion: For AGP patients, an SLS did not provide meaningful insight into hip and pelvis control or loading during sporting movements that are associated with injury development. The usefulness of an SLS test in the assessment of movement control and loading in AGP patients is thus limited. The SLS provided moderate insight into knee control while landing and therefore may be of use in the examination of knee-injury risk. [ABSTRACT FROM AUTHOR]

The aim of this study is to propose a novel data analysis approach, an analysis of characterizing phases (ACP), that detects and examines phases of variance within a sample of curves utilizing the time, magnitude, and magnitude-time domains; and to compare the findings of ACP to discrete point analysis in identifying performance-related factors in vertical jumps. Twenty-five vertical jumps were analyzed. Discrete point analysis identified the initial-to-maximum rate of force development (P = .006) and the time from initial-to-maximum force (P = .047) as performance-related factors. However, due to intersubject variability in the shape of the force curves (ie, non-, uni- and bimodal nature), these variables were judged to be functionally erroneous. In contrast, ACP identified the ability to apply forces for longer (P < .038), generate higher forces (P < .027), and produce a greater rate of force development (P < .003) as performance-related factors. Analysis of characterizing phases showed advantages over discrete point analysis in identifying performance-related factors because it (i) analyses only related phases, (ii) analyses the whole data set, (iii) can identify performance-related factors that occur solely as a phase, (iv) identifies the specific phase over which differences occur, and (v) analyses the time, magnitude and combined magnitude-time domains. [ABSTRACT FROM AUTHOR]