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Your search keyword '"Marquardt, Yvonne"' showing total 174 results
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174 results on '"Marquardt, Yvonne"'

7. Understanding the impact of risankizumab on keratinocyte-derived IL-23A in a novel organotypic 3D skin model containing IL-23A responsive and IL-17A producing γδ-T-cells.

Author

Huth, Laura, Amann, Philipp M., Marquardt, Yvonne, Jansen, Manuela, Baron, Jens Malte, and Huth, Sebastian

Subjects
KERATINOCYTE differentiation, GENE expression, FIBROBLASTS, T cells, KERATINOCYTES, MOLECULAR pathology, IN vitro studies
Abstract

To study the effects of the anti-IL-23A antibody risankizumab on the IL-36γ/IL-23A/IL-17A signalling cascade we used a newly developed 3D skin model consisting of primary human keratinocytes, fibroblasts and γδ-T-cells. In this in vitro study we developed new full-thickness 3D skin models containing normal human epidermal keratinocytes (NHEK), normal human dermal fibroblasts (NHDF) and IL-23A responsive and IL-17A producing γδ-T-cells. The effects of IL-36γ stimulation with and without risankizumab treatment on IL-23A and IL-17A expression were examined at the RNA and protein levels. In preliminary monolayer experiments stimulation of γδ-T-cells with IL-23A promoted the IL-17A expression that was inhibited after risankizumab treatment. Using 3D skin models containing γδ-T-cells, we found that stimulation with IL-36γ significantly increased not only IL-23A but also IL-17A expression. These effects were inhibited by concomitant treatment with risankizumab. Our results showed that blockade of IL-23A has inhibitory effects on the IL-36γ/IL-23A feedforward loop. Our newly developed 3D skin model containing IL-23A responsive and IL-17A producing γδ-T-cells enables molecular analysis of targeted therapies aimed at the IL-36γ/IL-23A/IL-17A signalling cascade in psoriasis. [ABSTRACT FROM AUTHOR]

Published
2024
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9. Macrophage‐like rapid uptake and toxicity of tattoo ink in human monocytes.

Author

Lin, Cheng, Marquardt, Yvonne, Rütten, Stefan, Liao, Liangliang, Rahimi, Khosrow, Haraszti, Tamas, Baron, Jens Malte, and Bartneck, Matthias

Subjects
*MONOCYTES, *TATTOOING, *CELL analysis, *INK, *SCANNING electron microscopy
Abstract

Macrophages play a critical role for the persistence of tattoo ink in human skin. However, a comparison to other skin‐resident and blood circulating immune cells and a profound analysis of REACH‐compliant tattoo ink are unmet medical needs. We hence characterized the size distribution of ink particles using physicochemical methods. We studied the uptake of tattoo ink by key human skin cells and blood‐derived immune cells using optical and electron microscopy as well as flow cytometry. Scanning electron microscopy of ink revealed its crystalline structure, and a tendency towards aggregations was indicated by size changes upon diluting it. Flow cytometric analyses of skin and immune cells after incubation with tattoo ink demonstrated an increase in cellular granularity upon uptake and red ink additionally evoked fluorescent signals. Human macrophages were most potent in internalizing ink in full thickness 3D skin models. Macrophage cultures demonstrated that the ink did not lead to elevated inflammatory mediators, and showed no indications for toxicity, even after nice days. Strikingly, monocytes were most efficient in ink uptake, but displayed reduced viability, whereas granulocytes and lymphocytes showed only temporary ink uptake with flow cytometric signals declining after 1 day. Mechanistic studies on ink retention by corticosteroids or dexpanthenol in macrophage cultures demonstrated that these compounds do not lead to ink excretion, but even slightly increase the ink load in macrophages. The highly motile monocytes, precursors of macrophages, may play an underrated role for tattoo ink translocation from dermal blood vessels into internal organs. [ABSTRACT FROM AUTHOR]

Published
2024
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11. Post-Treatment of Micro-Needling with a Dexpanthenol-Containing Ointment Accelerates Epidermal Wound Healing in Human 3D Skin Models

Author

Weßollek,Katharina, Marquardt,Yvonne, Wagner-Schiffler,Sylvia, Baron,Jens Malte, Huth,Sebastian, Weßollek,Katharina, Marquardt,Yvonne, Wagner-Schiffler,Sylvia, Baron,Jens Malte, and Huth,Sebastian

Abstract

Katharina Weßollek,1 Yvonne Marquardt,1 Sylvia Wagner-Schiffler,2 Jens Malte Baron,1,* Sebastian Huth1,* 1Department of Dermatology and Allergology, University Hospital RWTH Aachen, Aachen, Germany; 2Praxis für Dermatologie, Aachen, Germany*These authors contributed equally to this workCorrespondence: Katharina Weßollek, Department of Dermatology and Allergology, University Hospital RWTH Aachen, Aachen, Germany, Tel +49 241 7501 8240, Fax +49 241 7501 8290, Email kwessollek@ukaachen.dePurpose: In vitro study on the molecular effects of post-treatment after micro-needling applications with a dexpanthenol-containing ointment (DCO) using 3D skin models.Patients and Methods: In this in vitro study, full-thickness human 3D skin models were treated with a micro-needling device according to its clinical application. For post-treatment, some of the models were additionally treated with a dexpanthenol-containing ointment (DCO). Histological samples were taken at 0, 24 and 48 hours. Gene expression analysis was performed after 24 hours.Results: Histological examination showed that DCO post-treated 3D skin models revealed a completed wound closure 24 hours after the micro-needling procedure. In contrast, DCO-untreated models still clearly exhibited the micro-needling lesions after the same period of time. After 48 hours, all models revealed a completed wound healing. In skin models that received micro-needling but no post-treatment with DCO, microarray analysis identified an upregulation of proinflammatory cytokines and chemokines and a downregulation of skin barrier and differentiation markers. In contrast, post-treatment with DCO leads to accelerated wound healing without affecting the initial inflammatory response caused by micro-needling, which leads to the subsequent collagen expression. This data was supported by qRT-PCR analyses.Conclusion: Post-treatment with DCO accelerates epidermal wound healing after micro-needling of 3D skin models witho

Published
2023

13. Post-Treatment of Micro-Needling with a Dexpanthenol-Containing Ointment Accelerates Epidermal Wound Healing in Human 3D Skin Models.

Author

Weßollek, Katharina, Marquardt, Yvonne, Wagner-Schiffler, Sylvia, Baron, Jens Malte, and Huth, Sebastian

Subjects
WOUND healing, SKIN tests, GENE expression, PATIENT aftercare, CLINICAL medicine, INFLAMMATION
Abstract

Purpose: In vitro study on the molecular effects of post-treatment after micro-needling applications with a dexpanthenol-containing ointment (DCO) using 3D skin models. Patients and Methods: In this in vitro study, full-thickness human 3D skin models were treated with a micro-needling device according to its clinical application. For post-treatment, some of the models were additionally treated with a dexpanthenol-containing ointment (DCO). Histological samples were taken at 0, 24 and 48 hours. Gene expression analysis was performed after 24 hours. Results: Histological examination showed that DCO post-treated 3D skin models revealed a completed wound closure 24 hours after the micro-needling procedure. In contrast, DCO-untreated models still clearly exhibited the micro-needling lesions after the same period of time. After 48 hours, all models revealed a completed wound healing. In skin models that received micro-needling but no post-treatment with DCO, microarray analysis identified an upregulation of proinflammatory cytokines and chemokines and a downregulation of skin barrier and differentiation markers. In contrast, post-treatment with DCO leads to accelerated wound healing without affecting the initial inflammatory response caused by micro-needling, which leads to the subsequent collagen expression. This data was supported by qRT-PCR analyses. Conclusion: Post-treatment with DCO accelerates epidermal wound healing after micro-needling of 3D skin models without impairing the immunostimulatory properties of micro-needling. These findings can help to optimise the aftercare routine after micro-needling procedures and to shorten the downtime for the patient after treatment. [ABSTRACT FROM AUTHOR]

Published
2023
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21. Molecular effects of photon irradiation and subsequent aftercare treatment with dexpanthenol‐containing ointment or liquid in 3D models of human skin and non‐keratinized oral mucosa

Author

Huth, Sebastian, primary, Marquardt, Yvonne, additional, Huth, Laura, additional, Schmitt, Laurenz, additional, Prescher, Kirsten, additional, Winterhalder, Philipp, additional, Steiner, Timm, additional, Hölzle, Frank, additional, Eble, Michael, additional, and Malte Baron, Jens, additional

Published
2021
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41. Macrophage migration inhibitory factor protects from nonmelanoma epidermal tumors by regulating the number of antigen-presenting cells in skin

Author

Brocks, Tania, Fedorchenko, Oleg, Schliermann, Nicola, Stein, Astrid, Moll, Ute M., Seegobin, Seth, Dewor, Manfred, Hallek, Michael, Marquardt, Yvonne, Fietkau, Katharina, Heise, Ruth, Huth, Sebastian, Pfister, Herbert, Bernhagen, Juergen, Bucala, Richard, Baron, Jens M., Fingerle-Rowson, Guenter, Brocks, Tania, Fedorchenko, Oleg, Schliermann, Nicola, Stein, Astrid, Moll, Ute M., Seegobin, Seth, Dewor, Manfred, Hallek, Michael, Marquardt, Yvonne, Fietkau, Katharina, Heise, Ruth, Huth, Sebastian, Pfister, Herbert, Bernhagen, Juergen, Bucala, Richard, Baron, Jens M., and Fingerle-Rowson, Guenter

Abstract

The response of the skin to harmful environmental agents is shaped decisively by the status of the immune system. Keratinocytes constitutively express and secrete the chemokine-like mediator, macrophage migration inhibitory factor (MIF), more strongly than dermal fibroblasts, thereby creating a MIF gradient in skin. By using global and epidermis-restricted Mif-knockout (Mif(-1-) and K14-Cre(+ltg) ;Mif(fllft)) mice, we found that MIF both recruits and maintains antigen-presenting cells in the dermis/epidermis. The reduced presence of antigen-presenting cells in the absence of MIF was associated with accelerated and increased formation of nonmelanoma skin tumors during chemical carcinogenesis. Our results demonstrate that MIF is essential for maintaining innate immunity in skin. Loss of keratinocyte-derived MIF leads to a loss of control of epithelial skin tumor formation in chemical skin carcinogenesis, which highlights an unexpected tumor-suppressive activity of MIF in murine skin.- Brocks, T., Fedorchenko, O., Schliermann, N., Stein, A., Moll, U. M., Seegobin, S., Dewor, M., Hallek, M., Marquardt, Y., Fietkau, K., Heise, R., Huth, S., Pfister, H., Bernhagen, J., Bucala, R., Baron, J. M., Fingerle-Rowson, G. Macrophage migration inhibitory factor protects from nonmelanoma epidermal tumors by regulating the number of antigen-presenting cells in skin.

Published
2017

43. Macrophage migration inhibitory factor protects from nonmelanoma epidermal tumors by regulating the number of antigen‐presenting cells in skin

Author

Brocks, Tania, primary, Fedorchenko, Oleg, additional, Schliermann, Nicola, additional, Stein, Astrid, additional, Moll, Ute M., additional, Seegobin, Seth, additional, Dewor, Manfred, additional, Hallek, Michael, additional, Marquardt, Yvonne, additional, Fietkau, Katharina, additional, Heise, Ruth, additional, Huth, Sebastian, additional, Pfister, Herbert, additional, Bernhagen, Juergen, additional, Bucala, Richard, additional, Baron,, Jens M., additional, and Fingerle‐Rowson, Guenter, additional

Published
2016
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46. Interferon Alpha Signalling and Its Relevance for the Upregulatory Effect of Transporter Proteins Associated with Antigen Processing (TAP) in Patients with Malignant Melanoma

Author

Heise, Ruth, primary, Amann, Philipp M., additional, Ensslen, Silke, additional, Marquardt, Yvonne, additional, Czaja, Katharina, additional, Joussen, Sylvia, additional, Beer, Daniel, additional, Abele, Rupert, additional, Plewnia, Gabriele, additional, Tampé, Robert, additional, Merk, Hans F., additional, Hermanns, Heike M., additional, and Baron, Jens M., additional

Published
2016
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49. Downregulation of STRA6 Expression in Epidermal Keratinocytes Leads to Hyperproliferation-Associated Differentiation in Both In Vitro and In Vivo Skin Models

Author

Skazik, Claudia, Amann, Philipp M., Heise, Ruth, Marquardt, Yvonne, Czaja, Katharina, Kim, Arianna, Ruehl, Ralph, Kurschat, Peter, Merk, Hans F., Bickers, David R., Baron, Jens M., Skazik, Claudia, Amann, Philipp M., Heise, Ruth, Marquardt, Yvonne, Czaja, Katharina, Kim, Arianna, Ruehl, Ralph, Kurschat, Peter, Merk, Hans F., Bickers, David R., and Baron, Jens M.

Abstract

Retinoids are known to affect skin cell proliferation and differentiation and are key molecules that target retinoid and retinoic acid receptors (RXRs and RARs), leading to physiological and pharmacologic effects. Our aim was to elucidate the role of the retinol-binding protein receptor STRA6, mediating cellular uptake of retinol, on skin structure and function. Our results indicate that STRA6 is constitutively expressed in human epidermal keratinocytes and dermal fibroblasts and is regulated via RAR/RXR-mediated pathways. HaCaT (Human adult low Calcium high Temperature) cells with stable STRA6 knockdown (STRA6KD) showed increased proliferation. Consistently, human organotypic 3D skin models using stable STRA6KD HaCaT cells showed a significantly thicker epidermis and enhanced expression of activation, differentiation, and proliferation markers. The effects were reversible after treatment with free retinol. Human skin reconstitution employing STRA6KD HaCaT cells leads to massive epithelial thickening under in vivo conditions in SCID mice. We propose that STRA6KD could lead to cellular vitamin A deficiency in keratinocytes. Consequently, STRA6 has a role for regulating retinoid homeostasis and in helping to program signaling that drives proliferation and differentiation of human skin cells. By its influence on hyperproliferation-associated differentiation, STRA6 could also have a role in skin regeneration and could be a target for pharmacological approaches to improve wound healing.

Published
2014

50. Downregulation of STRA6 Expression in Epidermal Keratinocytes Leads to Hyperproliferation-Associated Differentiation in Both In Vitro and In Vivo Skin Models

Author

Skazik, Claudia, primary, Amann, Philipp M., additional, Heise, Ruth, additional, Marquardt, Yvonne, additional, Czaja, Katharina, additional, Kim, Arianna, additional, Rühl, Ralph, additional, Kurschat, Peter, additional, Merk, Hans F., additional, Bickers, David R., additional, and Baron, Jens M., additional

Published
2014
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