Search

All three hitherto published novels by Dana Grigorcea do explicitly refer to Romania. Had her first novel been set in the Danube Delta and her second in Bucharest, so the plot of the recently released novel Die nicht sterben is located in the touristic town B. (= Buşteni) at the foot of the Carpathian Mountains. Based on Bram Stoker’s Dracula as literary pre-text, the plot of Die nicht sterben interweaves elements of Romanian history, Romanian contemporary events as well as elements of the family history of the first-person narrator. The present paper is focused especially on the female narrator’s bodily, erotic and flying fantasies. The social and moral revolt which manifests itself first and foremost in the vampiresses’ urge to impale, subsides in the end in uncritical idyllic and narcissistic self-reflection.

In modified production landscapes, biodiversity faces unprecedented pressures from human actions, resulting in significant species declines of plant and animal taxa, including birds. Understanding the underlying mechanisms responsible for such declines is essential to counteract further loss and support practitioners in conserving biodiversity and associated ecosystem function. In this study, we used standardized bird monitoring data collected over 6 years in managed forest and grassland areas across different regions in Germany, Central Europe. We combined these data with morphometric, ecological, behavioral, and acoustic trait data and detailed information on local land use management practices to understand how management decisions affect species and functional diversity, as well as ecological processes shaping local species composition. Our results reveal that the ecosystem and regional context must be considered to understand how management practices affect bird diversity aspects and composition. In forests, regional management decisions related to tree species and stand age affected bird diversity, as well as community and functional composition, and indicated environmental sorting due to ecological and behavioral requirements, biotic interactions, and morphometric constraints. In grasslands, independent of local management practices, increased intensity of land use resulted in an overall loss in bird species richness and functional diversity. Predominantly, constraints due to ecological or behavioral requirements affected bird species assemblage composition. In addition, our results indicated the importance of woody vegetation near managed grasslands and of considering environmental conditions beyond the local scale to support bird diversity and associated ecosystem functions. Our results highlighted that local management decisions can support bird diversity and maintain ecological function. However, this needs a view beyond the local scale of management units. It also demands a joint effort of biologists and land managers to integrate targeted conservation actions into regional management practices and create a network of habitats within production landscapes to protect nature, guard against biotic and functional homogenization, and prevent further degradation of ecosystems in production landscapes.

Abstract Plant diversity effects on community productivity often increase over time. Whether the strengthening of diversity effects is caused by temporal shifts in species-level overyielding (i.e., higher species-level productivity in diverse communities compared with monocultures) remains unclear. Here, using data from 65 grassland and forest biodiversity experiments, we show that the temporal strength of diversity effects at the community scale is underpinned by temporal changes in the species that yield. These temporal trends of species-level overyielding are shaped by plant ecological strategies, which can be quantitatively delimited by functional traits. In grasslands, the temporal strengthening of biodiversity effects on community productivity was associated with increasing biomass overyielding of resource-conservative species increasing over time, and with overyielding of species characterized by fast resource acquisition either decreasing or increasing. In forests, temporal trends in species overyielding differ when considering above- versus belowground resource acquisition strategies. Overyielding in stem growth decreased for species with high light capture capacity but increased for those with high soil resource acquisition capacity. Our results imply that a diversity of species with different, and potentially complementary, ecological strategies is beneficial for maintaining community productivity over time in both grassland and forest ecosystems.

Abstract Background In this case series, a perioperative anticoagulation protocol for microvascular head and neck surgery in patients with thrombophilia is presented. Microvascular free-flap surgery is a standard procedure in head and neck surgery with high success rates. Nevertheless, flap loss—which is most often caused by thrombosis—can occur and has far-reaching consequences, such as functional impairment, prolonged hospitalization, and increased costs. The risk of flap loss owing to thrombosis is significantly increased in patients with thrombophilia. Therefore, perioperative anticoagulation is mandatory. To date, no perioperative anticoagulation protocol exists for these high-risk patients. Case presentation We present three exemplary male Caucasian patients aged 53–57 years with free flap loss owing to an underlying, hidden thrombophilia. Conclusion We present a modified anticoagulation protocol for microvascular surgery in these high-risk patients, enabling successful microsurgical reconstruction.

Abstract Organismal functional strategies form a continuum from slow- to fast-growing organisms, in response to common drivers such as resource availability and disturbance. However, whether there is synchronisation of these strategies at the entire community level is unclear. Here, we combine trait data for >2800 above- and belowground taxa from 14 trophic guilds spanning a disturbance and resource availability gradient in German grasslands. The results indicate that most guilds consistently respond to these drivers through both direct and trophically mediated effects, resulting in a ‘slow-fast’ axis at the level of the entire community. Using 15 indicators of carbon and nutrient fluxes, biomass production and decomposition, we also show that fast trait communities are associated with faster rates of ecosystem functioning. These findings demonstrate that ‘slow’ and ‘fast’ strategies can be manifested at the level of whole communities, opening new avenues of ecosystem-level functional classification.

Local abundance and regional distribution are two aspects of a species’ rarity. They are suggested to differentially alter genetic processes in plants: Locally rare species are hypothesized to suffer less from inbreeding and outbreeding than locally common species, thanks to genetic purging through long inbreeding histories and weaker local adaptations, respectively. Regionally rare species are hypothesized to be more susceptible to outbreeding, but less to inbreeding, compared to regionally common ones, due to small and declining range size. While this has major implications for plant conservation practices, we lack evidences and general understanding on how breeding effects on a plant’s early life fitness are related to its local and regional rarity. To investigate effects of inbreeding and outbreeding on plants’ early fitness, we performed self-, within- and between-population pollinations in eight pairs of closely related species differing in regional and local rarity. To avoid biases due to context dependency, we took species competitive ability, habitat resource-richness and resource-allocation strategy into account in the analyses. We then tested how inbreeding and outbreeding affected five fruit-, seed- and seedling-related traits. Inbreeding did not generally have more negative effects on early fitness of regionally rare and non-competitive species than on regionally common and competitive ones. Outbreeding was generally beneficial to early fitness of plant species across the gradients of regional rarity, competitive ability and habitat resource-richness. Our results show that outbreeding may be beneficial to the early fitness of plant species, including rare and non-competitive ones and may be considered for conservation strategies.

Heterogeneity in forests might promote biodiversity not only through an increase in niche volume but also through other processes, such as an increase in resources and their spatial distribution. However, negative relationships between heterogeneity and biodiversity have also been observed, which may indicate that heterogeneity acts as a filter for some species. This study examined the effects of different facets of heterogeneity in forest stands, i.e. deadwood, plant diversity, forest stand structure, and micro-scale topography, on the ecological (functional-phylogenetic) diversity and redundancy of nine animal groups: moths, true bugs, different functional groups of beetles, spiders, birds, and bats. Overall, we found positive effects of heterogeneity on ecological diversity and redundancy. Although the effect of heterogeneity at the local scale was moderate compared with the general effect of region, greater heterogeneity could be beneficial to some species groups and lead to more resilient species communities.

Abstract Despite evidence from grasslands experiments suggesting that plant species loss reduces biomass production, the strength of biodiversity-ecosystem functioning relationships in managed grasslands is still debated. High land-use intensity and reduced species pools are often suggested to make relationships between biodiversity and productivity less positive or even negative, but concrete evidence is still scarce. We investigated biodiversity-productivity relationships over two years in 150 managed grasslands in Germany. Specifically, we distinguished between relationships of biodiversity and biomass production in managed grasslands (1) varying in land-use intensity (e.g. of mowing, grazing and/or fertilization), (2) where land-use intensity is experimentally reduced, and (3) where additionally to land-use reductions, species pools are enlarged by seed addition. Among grasslands varying in land-use intensity, we found negative biodiversity-productivity relationships. Land-use reduction weakened these relationships, towards neutral, and sometimes, even positive relationships. Seed addition reduced species pool limitations, but this did not strengthen biodiversity-productivity relationships. Our findings indicate that land-use intensity is an important factor explaining the predominantly negative biodiversity-productivity relationships in managed grasslands. While we did not find that species pool limitations weakened biodiversity-productivity relationships, our results are based on a two-year-old experiment, possibly such effects are only visible in the long-term. Ultimately, advancing insights on biodiversity-ecosystem functioning relationships helps us to understand under which conditions agricultural production may benefit from promoting biodiversity.

Abstract Prediction of chemical shift in NMR using machine learning methods is typically done with the maximum amount of data available to achieve the best results. In some cases, such large amounts of data are not available, e.g. for heteronuclei. We demonstrate a novel machine learning model that is able to achieve better results than other models for relevant datasets with comparatively low amounts of data. We show this by predicting $$^{19}F$$ 19 F and $$^{13}C$$ 13 C NMR chemical shifts of small molecules in specific solvents. Graphical Abstract

Element profiling is a powerful tool for detecting fraud related to claims of geographical origin. However, these methods must be continuously developed, as mixtures of different origins in particular offer great potential for adulteration. This study is a proof of principle to determine whether elemental profiling is suitable for detecting mixtures of the same food but from different origins and whether calculated data from walnut mixtures could help to reduce the measurement burden. The calculated data used in this study were generated based on measurements of authentic, unadulterated samples. Five different classification models and three regression models were applied in five different evaluation approaches to detect adulteration or even distinguish between adulteration levels (10% to 90%). To validate the method, 270 mixtures of walnuts from different origins were analyzed using inductively coupled plasma mass spectrometry (ICP-MS). Depending on the evaluation approach, different characteristics were observed in mixtures when comparing the calculated and measured data. Based on the measured data, it was possible to detect admixtures with an accuracy of 100%, even at low levels of adulteration (20%), depending on the country. However, calculated data can only contribute to the detection of adulterated walnut samples in exceptional cases.

This study investigates for the first time how public charging infrastructure usage differs under the presence of diverse pricing models. About 3 million charging events from different European countries were classified according to five different pricing models (cost-free, flat-rate, time-based, energy-based, and mixed) and evaluated using various performance indicators such as connection duration; transferred energy volumes; average power; achievable revenue; and the share of charging and idle time for AC, DC, and HPC charging infrastructure. The study results show that the performance indicators differed for the classified pricing models. In addition to the quantitative comparison of the performance indicators, a Kruskal–Wallis one-way analysis of variance and a pairwise comparison using the Mann–Whitney-U test were used to show that the data distributions of the defined pricing models were statistically significantly different. The results are discussed from various perspectives on the efficient design of public charging infrastructure. The results show that time-based pricing models can improve the availability of public charging infrastructure, as the connection duration per charging event can be roughly halved compared to other pricing models. Flat-rate pricing models and AC charging infrastructure can support the temporal shift of charging events, such as shifting demand peaks, as charging events usually have several hours of idle time per charging process. By quantifying various performance indicators for different charging technologies and pricing models, the study is relevant for stakeholders involved in the development and operation of public charging infrastructure.

The Mediterranean herb oregano is one of the most frequently adulterated foods. Often morphologically similar leaf material is used as a filler, which can generally be detected using DNA-based methods. Loop-mediated isothermal amplification (LAMP) has high potential for point-of-care testing as it requires only a simple device for sample incubation and is less sensitive to inhibition by co-isolated metabolites compared to conventional PCRs (polymerase chain reactions). In this work, we have developed two LAMP assays for the specific detection of the adulterants olive (Olea europaea) and strawberry tree (Arbutus unedo). The combination with a rapid isolation protocol and LFAs (Lateral-flow assays) as a visualization technique provides a reliable indication of possible adulteration. It has also been shown that it is possible to estimate the level of contamination and to perform the LAMP/LFA assay with DNA isolation in less than 30 min. As a further option, a duplex LAMP/LFA assay was developed that allows both contaminants to be detected in parallel, making the rapid test system even more cost-effective and user-friendly.

The diagnosis of celiac disease (CD) is complex and requires a multi-step procedure (symptoms, serology, duodenal biopsy, effect of a gluten-free diet, and optional genetic). The aim of the study was to contribute to the improvement of CD diagnosis by preparing a water-soluble gluten peptide fraction (called Solgluten) and by selecting gluten-specific enzyme-linked immunosorbent assays (ELISA) for the detection of gluten immunogenic gluten peptides (GIPs) in urine and blood serum spiked with Solgluten. Food-grade Solgluten was prepared by the extraction of a peptic digest of vital gluten with water, centrifugation, and freeze-drying. The process was relatively easy, repeatable, and cheap. The content of gliadin-derived GIPs was 491 mg/g. Solgluten was used as antigenic material to compare two competitive ELISA kits (R7021 and K3012) and two sandwich ELISA kits (M2114 and R7041) in their quality regarding the quantitation of GIPs in urine and blood serum. The quality parameters were the reactivity, sensitivity, coefficients of variation and determination, and curve shape. The evaluation of the kits showed a number of discrepancies in individual quality parameters measured in urine and serum. Due to the lowest limit of quantitation and the highest coefficient of determination, M2114 may be the first choice, while R7021 appeared to be less suitable because of the high coefficients of variation and unfavorable curve progression. The results set the stage for improving CD diagnosis by supplementing conventional blood tests with oral provocation with Solgluten and subsequent ELISA measurement of GIPs that could support the no-biopsy approach and by better assessing the effect of a gluten-free diet by monitoring adherence to the diet by measuring GIPs in urine and blood.

5-Deazaflavins are analogs of naturally occurring flavin cofactors. They serve as substitutes for natural flavin cofactors to investigate and modify the reaction pathways of flavoproteins. Demethylated 5-deazaflavins are potential candidates for artificial cofactors, allowing us to fine-tune the reaction kinetics and absorption characteristics of flavoproteins. In this contribution, demethylated 5-deazariboflavin radicals are investigated (1) to assess the influence of the methyl groups on the electronic structure of the 5-deazaflavin radical and (2) to explore their photophysical properties with regard to their potential as artificial cofactors. We determined the proton hyperfine structure of demethylated 5-deazariboflavins using photochemically induced dynamic nuclear polarization (photo-CIDNP) spectroscopy, as well as density functional theory (DFT). To provide context, we compare our findings to a study of flavin mononucleotide (FMN) derivatives. We found a significant influence of the methylation pattern on the absorption properties, as well as on the proton hyperfine coupling ratios of the xylene moiety, which appears to be solvent-dependent. This effect is enhanced by the replacement of N5 by C5-H in 5-deazaflavin derivatives compared to their respective flavin counterparts.

Abstract Rare plant species are suggested to be less resistant to herbivores than common species. Their lower apparency and the fact that they often live in isolated populations, resulting in fewer herbivore encounters, might have led to the evolution of reduced defences. Moreover, their frequent lower levels of genetic diversity compared with common species could negatively affect their resistance against enemies. However, the hypothesis that plant resistance depends on plant regional and local rarity, independently of habitat and competitive and growth strategy, lacks evidence. To test this hypothesis, we assessed the performance and preference of one belowground and three aboveground generalist invertebrate herbivores from different taxonomic groups as indicators of plant resistance. Herbivores were fed a total of 62 regionally and locally rare and common plant species from Switzerland. We accounted for differences in a plant's growth and competitive strategy and habitat resource availability. We found that regionally and locally rare and common plant species did not generally differ in their resistance to most generalist herbivores. However, one herbivore species even performed better and preferred locally and regionally common plant species over rarer ones, indicating that common species are not more resistant, but tend to be less resistant. We also found that all herbivore species consistently performed better on competitive and large plant species, although different herbivore species generally preferred and performed better on different plant species. The latter indicates that the use of generalist herbivores as indicators of plant‐resistance levels can be misleading. Synthesis: Our results show that rare plant species are not inherently less resistant than common ones to herbivores. Instead, our results suggest that the ability of plants to allocate resources away from defence towards enhancing their competitive ability might have allowed plants to tolerate herbivory, and to become locally and regionally common.

IntroductionFontan-palliated patients are at risk for the development of Fontan-associated liver disease (FALD). Currently, there is no consensus on how to stage FALD. Transient elastography (TE) is a rapid, non-invasive method to assess FALD and liver fibrosis.MethodTo assess the availability and conditions of using TE to monitor liver disease in Fontan patients in german centers for pediatric cardiology and to propose the introduction of a standardized national protocol for the monitoring of liver disease, we developed a questionnaire.ResultsIn total, 95 valid questionnaires were collected. Only 20% of the centers offer the TE investigation directly. Most of the centers transfer the patients to another department or center (40%) or didńt offer TE (40%). In only 2.6% of the centers TE is performed directly by the cardiologist. Most of the centers transfer the patients to a other department. In 29.2% TE is performed only at a certain age of the patients and in 27.7% it is performed if the patients present symptoms of failing Fontan. In only 13.9% of the centers TE is proposed in all the Fontan patients on a routine basis. Most often TE is performed only from the beginning of the adolescence. In the majority of answers it was not known if the patients are fasting for the examination (68%) or not and if the TE examination had to be performed in a specific breathing phase during TE (Inspiration/Expiration, 90%). In the majority, TE is not offered routinely (46.9%).DiscussionTo date in Germany, TE is only used in a few numbers of centers specialized in Fontan follow-up. A standardized protocol to use TE is currently not existing. With regard to the feasibility of the examination, it is evident that TE is a quick, cheap and easy method to distinguish between cases with and without progressive FALD. This makes TE a useful and prognostic tool for screening of liver disease and to failing Fontan circulation.ConclusionWe propose a systematic TE evaluation of possible liver congestion and fibrosis, as a part of the routine follow-up of Fontan patients.

Biodiversity-ecosystem functioning relationships may change over time. Here, Wagg et al. show that richness-productivity and richness stability relationships grow stronger over time in an experimental grassland community, and shed light on the ecological mechanisms.

The untargeted metabolomics analysis of biological samples with nuclear magnetic resonance (NMR) provides highly complex data containing various signals from different molecules. To use these data for classification, e.g., in the context of food authentication, machine learning methods are used. These methods are usually applied as a black box, which means that no information about the complex relationships between the variables and the outcome is obtained. In this study, we show that the random forest-based approach surrogate minimal depth (SMD) can be applied for a comprehensive analysis of class-specific differences by selecting relevant variables and analyzing their mutual impact on the classification model of different truffle species. SMD allows the assignment of variables from the same metabolites as well as the detection of interactions between different metabolites that can be attributed to known biological relationships.

Abstract Effective approaches towards sustainability need to be informed by a diverse array of stakeholder perspectives. However, capturing these perspectives in a way that can be integrated with other forms of knowledge can represent a challenge. Here we present the first application of the conceptual framework of the Intergovernmental Science‐Policy Platform on Biodiversity and Ecosystem Services (IPBES) to a participatory assessment of local perspectives on nature, people and sustainability on Mount Kilimanjaro, Tanzania. This assessment was organized in the form of a participatory workshop with five different groups of stakeholders. Following this framework, we assembled information on the state of and trends in species diversity, Nature's Contributions to People (NCP), and on the main drivers of changes in species and habitats. Additionally, we gathered perspectives on the needs and opportunities for the sustainable management and conservation of natural resources from the individual to the international level. The various stakeholders agreed that both the condition and extent of the various habitats and NCP are declining. In line with available knowledge, the key direct drivers of change mentioned by the workshop participants were land use and climate change, whereas human population growth was singled out as the most important indirect driver. The most frequently suggested measures to address the observed decline in species diversity and its drivers were related to land and water management and to education and awareness raising. Yet, the stakeholder groups differed in the measures they suggested. The willingness of a diversity of knowledge holders to systematically engage in a structured discussion around all the elements of the IPBES framework provides support for its applicability in participatory workshops aimed at capturing nuanced and context‐based perspectives on social–ecological systems from informed stakeholders. The application of the IPBES framework enabled the comparability needed for developing narratives of stakeholder visions that can help identify new pathways towards sustainability and guide planning while retaining the context‐based nuances that remain unresolved with non‐participatory methods. Read the free Plain Language Summary for this article on the Journal blog.

Measurement(s) mountain range Technology Type(s) Geographic Information System Factor Type(s) ruggedness Sample Characteristic - Environment mountain Sample Characteristic - Location global

Accelerated storage is routinely used with pharmaceuticals to predict stability and degradation patterns over time. The aim of this is to assess the shelf life and quality under harsher conditions, providing crucial insights into their long-term stability and potential storage issues. This study explores the potential of transferring this approach to food matrices for shelf-life estimation. Therefore, hazelnuts were stored under accelerated short-term and realistic long-term conditions. Subsequently, they were analyzed with high resolution mass spectrometry, focusing on the lipid profile. LC-MS analysis has shown that many unique processes take place under accelerated conditions that do not occur or occur much more slowly under realistic conditions. This mainly involved the degradation of membrane lipids such as phospholipids, ceramides, and digalactosyldiacylglycerides, while oxidation processes occurred at different rates in both conditions. It can be concluded that a food matrix is far too complex and heterogeneous compared to pharmaceuticals, so that many more processes take place during accelerated storage, which is why the results cannot be used to predict molecular changes in hazelnuts stored under realistic conditions.

Nutmeg is a popular spice often used in ground form, which makes it highly susceptible to food fraud. Therefore, the aim of the present study was to detect adulteration of ground nutmeg with nutmeg shell via Fourier transform near-infrared (FT-NIR) spectroscopy. For this purpose, 36 authentic nutmeg samples and 10 nutmeg shell samples were analyzed pure and in mixtures with up to 50% shell content. The spectra plot as well as a principal component analysis showed a clear separation trend as a function of shell content. A support vector machine regression used for shell content prediction achieved an R2 of 0.944 in the range of 0–10%. The limit of detection of the prediction model was estimated to be 1.5% nutmeg shell. Based on random sub-sampling, the likelihood was found to be 2% that a pure nutmeg sample is predicted with a nutmeg shell content of >1%. The results confirm the suitability of FT-NIR spectroscopy for rapid detection and quantitation of the shell content in ground nutmeg.

The importance of animal welfare and the organic production of chicken eggs has increased in the European Union in recent years. Legal regulation for organic husbandry makes the production of organic chicken eggs more expensive compared to conventional husbandry and thus increases the risk of food fraud. Therefore, the aim of this study was to develop a non-targeted lipidomic LC-ESI-IM-qToF-MS method based on 270 egg samples, which achieved a classification accuracy of 96.3%. Subsequently, surrogate minimal depth (SMD) was applied to select important variables identified as carotenoids and lipids based on their MS/MS spectra. The LC-MS results were compared with FT-NIR spectroscopy analysis as a low-resolution screening method and achieved 80.0% accuracy. Here, SMD selected parts of the spectrum which are associated with lipids and proteins. Furthermore, we used SMD for low-level data fusion to analyze relations between the variables of the LC-MS and the FT-NIR spectroscopy datasets. Thereby, lipid-associated bands of the FT-NIR spectrum were related to the identified lipids from the LC-MS analysis, demonstrating that FT-NIR spectroscopy partially provides similar information about the lipidome. In future applications, eggs can therefore be analyzed with FT-NIR spectroscopy to identify conspicuous samples that can subsequently be counter-tested by mass spectrometry.

Abstract Flavocoenzymes are nearly ubiquitous cofactors that are involved in the catalysis and regulation of a wide range of biological processes including some light-induced ones, such as the photolyase-mediated DNA repair, magnetoreception of migratory birds, and the blue-light driven phototropism in plants. One of the factors that enable versatile flavin-coenzyme biochemistry and biophysics is the fine-tuning of the cofactor’s frontier orbital by interactions with the protein environment. Probing the singly-occupied molecular orbital (SOMO) of the intermediate radical state of flavins is therefore a prerequisite for a thorough understanding of the diverse functions of the flavoprotein family. This may be ultimately achieved by unravelling the hyperfine structure of a flavin by electron paramagnetic resonance. In this contribution we present a rigorous approach to obtaining a hyperfine map of the flavin’s chromophoric 7,8-dimethyl isoalloxazine unit at an as yet unprecedented level of resolution and accuracy. We combine powerful high-microwave-frequency/high-magnetic-field electron–nuclear double resonance (ENDOR) with 13C isotopologue editing as well as spectral simulations and density functional theory calculations to measure and analyse 13C hyperfine couplings of the flavin cofactor in DNA photolyase. Our data will provide the basis for electronic structure considerations for a number of flavin radical intermediates occurring in blue-light photoreceptor proteins.

This review explains the strategies behind genomics, proteomics, metabolomics, metallomics and isotopolomics approaches and their applicability to written artefacts. The respective sub-chapters give an insight into the analytical procedure and the conclusions drawn from such analyses. A distinction is made between information that can be obtained from the materials used in the respective manuscript and meta-information that cannot be obtained from the manuscript itself, but from residues of organisms such as bacteria or the authors and readers. In addition, various sampling techniques are discussed in particular, which pose a special challenge in manuscripts. The focus is on high-resolution, non-targeted strategies that can be used to extract the maximum amount of information about ancient objects. The combination of the various omics disciplines (panomics) especially offers potential added value in terms of the best possible interpretations of the data received. The information obtained can be used to understand the production of ancient artefacts, to gain impressions of former living conditions, to prove their authenticity, to assess whether there is a toxic hazard in handling the manuscripts, and to be able to determine appropriate measures for their conservation and restoration.

Oregano (Origanum vulgare and O. onites) is one of the most frequently counterfeited herbs in the world and is diluted with the leaves of a wide variety of plants. In addition to olive leaves, marjoram (O. majorana) is often used for this purpose in order to achieve a higher profit. However, apart from arbutin, no marker metabolites are known to reliably detect marjoram admixtures in oregano batches at low concentrations. In addition, arbutin is relatively widespread in the plant kingdom, which is why it is of great relevance to look for further marker metabolites in order to secure the analysis accordingly. Therefore, the aim of the present study was to use a metabolomics-based approach to identify additional marker metabolites with the aid of an ion mobility mass spectrometry instrument. The focus of the analysis was on the detection of non-polar metabolites, as this study was preceded by nuclear magnetic resonance spectroscopic investigations of the same samples based mainly on the detection of polar analytes. Using the MS-based approach, numerous marjoram specific features could be detected in admixtures of marjoram >10% in oregano. However, only one feature was detectable in admixtures of >5% marjoram. This feature was identified as blumeatin, which belongs to the class of flavonoid compounds. Initially, blumeatin was identified based on MS/MS spectra and collision cross section values using a database search. In addition, the identification of blumeatin was confirmed by a reference standard. Moreover, dried leaves of olive, myrtle, thyme, sage and peppermint, which are also known to be used to adulterate oregano, were measured. Blumeatin could not be detected in these plants, so this substance can be considered as an excellent marker compound for the detection of marjoram admixtures.

In this proof-of-principle study, we systematically studied the potential of Raman spectroscopy for detecting pre-analytical delays in blood serum samples. Spectra from 330 samples from a liver cirrhosis cohort were acquired over the course of eight days, stored one day at room temperature, and stored subsequently at 4 °C. The spectra were then used to train Convolutional Neural Networks (CNN) to predict the delay to sample examination. We achieved 90% accuracy for binary classification of the serum samples in the groups “without delay” versus “delayed”. Spectra recorded on the first day could be distinguished clearly from all subsequent measurements. Distinguishing between spectra taken in the range from the second to the last day seems to be possible as well, but currently, with an accuracy of approximately 70% only. Importantly, filtering out the fluorescent background significantly reduces the precision of detection.

Land use intensification is a major driver of biodiversity change. Here the authors measure diversity across multiple trophic levels in agricultural grassland landscapes of varying management, finding decoupled responses of above- and belowground taxa to local factors and a strong impact of landscape-level land use.

Interactions of ionic liquids (ILs) with phospholipid membranes play a crucial role in their biological activity be it their cytotoxicity or their application as drug delivery agents. To develop the design principles for task-specific applications of ionic liquids, structural details of this interaction with atomic resolution must be obtained. In this context, 31P and 2H-based solid-state NMR spectroscopy of phospholipid membranes is a very useful technique. Here, we have used it to study the impact of benzimidazolium (BNZ) and morpholinium (MPH) cation-based ionic liquids on the head-group orientation and acyl chain disorder of negatively charged POPG membranes. Owing to the large-size and aromatic nature of its head group, membrane partitioning of BNZ cations increases the disorder of the entire POPG acyl chain. Comparisons are drawn with the behavior of widely studied imidazolium (IMI) cation which, like MPH, has a contrasting impact in the upper and lower halves of POPG chains. The observations are used to rationalize the higher potential of BNZ to permeabilize and fuse POPG vesicles, which was determined using fluorescence-based dye leakage and lipid mixing assays.

Abstract Plant‐soil feedbacks (PSFs) have been shown to strongly affect plant performance under controlled conditions, and PSFs are thought to have far reaching consequences for plant population dynamics and the structuring of plant communities. However, thus far the relationship between PSF and plant species abundance in the field is not consistent. Here, we synthesize PSF experiments from tropical forests to semiarid grasslands, and test for a positive relationship between plant abundance in the field and PSFs estimated from controlled bioassays. We meta‐analyzed results from 22 PSF experiments and found an overall positive correlation (0.12 ≤ r¯ ≤ 0.32) between plant abundance in the field and PSFs across plant functional types (herbaceous and woody plants) but also variation by plant functional type. Thus, our analysis provides quantitative support that plant abundance has a general albeit weak positive relationship with PSFs across ecosystems. Overall, our results suggest that harmful soil biota tend to accumulate around and disproportionately impact species that are rare. However, data for the herbaceous species, which are most common in the literature, had no significant abundance‐PSFs relationship. Therefore, we conclude that further work is needed within and across biomes, succession stages and plant types, both under controlled and field conditions, while separating PSF effects from other drivers (e.g., herbivory, competition, disturbance) of plant abundance to tease apart the role of soil biota in causing patterns of plant rarity versus commonness.

Abstract Background Forests perform various important ecosystem functions that contribute to ecosystem services. In many parts of the world, forest management has shifted from a focus on timber production to multi-purpose forestry, combining timber production with the supply of other forest ecosystem services. However, it is unclear which forest types provide which ecosystem services and to what extent forests primarily managed for timber already supply multiple ecosystem services. Based on a comprehensive dataset collected across 150 forest plots in three regions differing in management intensity and species composition, we develop models to predict the potential supply of 13 ecosystem services. We use those models to assess the level of multifunctionality of managed forests at the national level using national forest inventory data. Results Looking at the potential supply of ecosystem services, we found trade-offs (e.g. between both bark beetle control or dung decomposition and both productivity or soil carbon stocks) as well as synergies (e.g. for temperature regulation, carbon storage and culturally interesting plants) across the 53 most dominant forest types in Germany. No single forest type provided all ecosystem services equally. Some ecosystem services showed comparable levels across forest types (e.g. decomposition or richness of saprotrophs), while others varied strongly, depending on forest structural attributes (e.g. phosphorous availability or cover of edible plants) or tree species composition (e.g. potential nitrification activity). Variability in potential supply of ecosystem services was only to a lesser extent driven by environmental conditions. However, the geographic variation in ecosystem function supply across Germany was closely linked with the distribution of main tree species. Conclusions Our results show that forest multifunctionality is limited to subsets of ecosystem services. The importance of tree species composition highlights that a lack of multifunctionality at the stand level can be compensated by managing forests at the landscape level, when stands of complementary forest types are combined. These results imply that multi-purpose forestry should be based on a variety of forest types requiring coordinated planning across larger spatial scales.

Aptamers offer several advantages over antibodies. However, to ensure high affinity and specificity, a better understanding of the interactions between the nucleic-acid-based aptamers and their targets is mandatory. Therefore, we investigated the influence of two physical properties of proteins—molecular mass and charge—on the affinity of nucleic-acid-based aptamers. For this purpose, first, the affinity of two random oligonucleotides towards twelve proteins was determined. No binding was observed for proteins with a negative net charge towards the two oligonucleotides, while up to nanomolar affinity was determined for positively charged proteins with a high pI value. Second, a literature analysis comprising 369 aptamer–peptide/protein pairs was performed. The dataset included 296 different target peptides and proteins and is thus currently one of the largest databases for aptamers for proteins and peptides. The targets considered covered isoelectric points of 4.1–11.8 and a molecular weight range of 0.7–330 kDa, while the dissociation constants ranged from 50 fM to 29.5 µM. This also revealed a significant inverse correlation between the protein’s isoelectric point and the affinity of aptamers. In contrast, no trend was observed between the affinity and the molecular weight of the target protein with either approach.

Acidobacteria occur in a large variety of ecosystems worldwide and are particularly abundant and highly diverse in soils. In spite of their diversity, only few species have been characterized to date which makes Acidobacteria one of the most poorly understood phyla among the domain Bacteria. We used a culture-independent niche modeling approach to elucidate ecological adaptations and their evolution for 4,154 operational taxonomic units (OTUs) of Acidobacteria across 150 different, comprehensively characterized grassland soils in Germany. Using the relative abundances of their 16S rRNA gene transcripts, the responses of active OTUs along gradients of 41 environmental variables were modeled using hierarchical logistic regression (HOF), which allowed to determine values for optimum activity for each variable (niche optima). By linking 16S rRNA transcripts to the phylogeny of full 16S rRNA gene sequences, we could trace the evolution of the different ecological adaptations during the diversification of Acidobacteria. This approach revealed a pronounced ecological diversification even among acidobacterial sister clades. Although the evolution of habitat adaptation was mainly cladogenic, it was disrupted by recurrent events of convergent evolution that resulted in frequent habitat switching within individual clades. Our findings indicate that the high diversity of soil acidobacterial communities is largely sustained by differential habitat adaptation even at the level of closely related species. A comparison of niche optima of individual OTUs with the phenotypic properties of their cultivated representatives showed that our niche modeling approach (1) correctly predicts those physiological properties that have been determined for cultivated species of Acidobacteria but (2) also provides ample information on ecological adaptations that cannot be inferred from standard taxonomic descriptions of bacterial isolates. These novel information on specific adaptations of not-yet-cultivated Acidobacteria can therefore guide future cultivation trials and likely will increase their cultivation success.

Deadwood is an important structural and functional component of forest ecosystems and biodiversity. As deadwood can make up large portions of the total aboveground biomass, it plays an important role in the terrestrial carbon (C) cycle. Nevertheless, in tropical ecosystems and especially in Africa, quantitative studies on this topic remain scarce. We conducted an aboveground deadwood inventory along two environmental gradients—elevation and land use— at Mt. Kilimanjaro, Tanzania. We used a huge elevation gradient (3690 m) along the southern slope of the mountain to investigate how deadwood is accumulated across different climate and vegetation zones. We also compared habitats that differed from natural forsts in land-use intensity and disturbance history to assess anthropogenic influence on deadwood accumulation. In our inventory we distinguished coarse woody debris (CWD) from fine woody debris (FWD). Furthermore, we calculated the C and nitrogen (N) content of deadwood and how the C/N ratio varied with decomposition stages and elevation. Total amounts of aboveground deadwood ranged from 0.07 ± 0.04 to 73.78 ± 36.26 Mg ha–1 (Mean ± 1 SE). Across the elevation gradient, total deadwood accumulation was highest at mid-elevations and reached a near-zero minimum at very low and very high altitudes. This unimodal pattern was mainly driven by the corresponding amount of live aboveground biomass and the combined effects of decomposer communities and climate. Land-use conversion from natural forests into traditional homegardens and commercial plantations, in addition to frequent burning, significantly reduced deadwood biomass, but not past selective logging after 30 years of recovery time. Furthermore, we found that deadwood C content increased with altitude. Our study shows that environmental gradients, especially temperature and precipitation, as well as different anthropogenic disturbances can have considerable effects on both the quantity and composition of deadwood in tropical forests.

Satellite-borne radar systems are promising tools to obtain spatial habitat data with complete geographic coverage. Here the authors show that freely available Sentinel-1 radar data perform as well as standard airborne laser scanning data for mapping biodiversity of 12 taxa across temperate forests in Germany.

The functioning and service provisioning of ecosystems in the face of anthropogenic environmental and biodiversity change is a cornerstone of ecological research. The last three decades of biodiversity–ecosystem functioning (BEF) research have provided compelling evidence for the significant positive role of biodiversity in the functioning of many ecosystems. Despite broad consensus of this relationship, the underlying ecological and evolutionary mechanisms have not been well understood. This complicates the transition from a description of patterns to a predictive science. The proposed Research Unit aims at filling this gap of knowledge by applying novel experimental and analytical approaches in one of the longest-running biodiversity experiments in the world: the Jena Experiment. The central aim of the Research Unit is to uncover the mechanisms that determine BEF relationships in the short- and in the long-term. Increasing BEF relationships with time in long-term experiments do not only call for a paradigm shift in the appreciation of the relevance of biodiversity change, they likely are key to understanding the mechanisms of BEF relationships in general. The subprojects of the proposed Research Unit fall into two tightly linked main categories with two research areas each that aim at exploring variation in community assembly processes and resulting differences in biotic interactions as determinants of the long-term BEF relationship. Subprojects under “Microbial community assembly” and “Assembly and functions of animal communities” mostly focus on plant diversity effects on the assembly of communities and their feedback effects on biotic interactions and ecosystem functions. Subprojects under “Mediators of plant-biotic interactions” and “Intraspecific diversity and micro-evolutionary changes” mostly focus on plant diversity effects on plant trait expression and micro-evolutionary adaptation, and subsequent feedback effects on biotic interactions and ecosystem functions. This unification of evolutionary and ecosystem processes requires collaboration across the proposed subprojects in targeted plant and soil history experiments using cutting-edge technology and will produce significant synergies and novel mechanistic insights into BEF relationships. The Research Unit of the Jena Experiment is uniquely positioned in this context by taking an interdisciplinary and integrative approach to capture whole-ecosystem responses to changes in biodiversity and to advance a vibrant research field.

Pitavastatin is a statin drug that, by competitively inhibiting 3-hydroxy-3-methylglutaryl-coenzyme A reductase, can lower serum cholesterol levels of low-density lipoprotein (LDL) accompanied by side effects due to pleiotropic effects leading to statin intolerance. These effects can be explained by the lipophilicity of statins, which creates membrane affinity and causes statin localization in cellular membranes. In the current report, the interaction of pitavastatin with POPC model membranes and its influence on the membrane structure were investigated using 1H, 2H and 31P solid-state NMR spectroscopy. Our experiments show the average localization of pitavastatin at the lipid/water interface of the membrane, which is biased towards the hydrocarbon core in comparison to other statin molecules. The membrane binding of pitavastatin also introduced an isotropic component into the 31P NMR powder spectra, suggesting that some of the lamellar POPC molecules are converted into highly curved structures.

To address the continued rise of multi-drug-resistant microorganisms, the development of novel drugs with new modes of action is urgently required. While humans biosynthesize the essential isoprenoid precursors isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) via the established mevalonate pathway, pathogenic protozoa and certain pathogenic eubacteria use the less well-known methylerythritol phosphate pathway for this purpose. Important pathogens using the MEP pathway are, for example, Plasmodium falciparum, Mycobacterium tuberculosis, Pseudomonas aeruginosa and Escherichia coli. The enzymes of that pathway are targets for antiinfective drugs that are exempt from target-related toxicity. 2C-Methyl-D-erythritol 4-phosphate (MEP), the second enzyme of the non-mevalonate pathway, has been established as the molecular target of fosmidomycin, an antibiotic that has so far failed to be approved as an anti-infective drug. This review describes the development and anti-infective properties of a wide range of fosmidomycin derivatives synthesized over the last four decades. Here we discuss the DXR inhibitor pharmacophore, which comprises a metal-binding group, a phosphate or phosphonate moiety and a connecting linker. Furthermore, non-fosmidomycin-based DXRi, bisubstrate inhibitors and several prodrug concepts are described. A comprehensive structure–activity relationship (SAR) of nearly all inhibitor types is presented and some novel opportunities for further drug development of DXR inhibitors are discussed.

In this study, a framework regarding park and ride facilities is presented and demonstrated to evaluate different approaches of charging concepts. The innovation in this study is that the framework can be used to evaluate arbitrary conductive charging concepts on a detailed level and on the basis of real usage data. Thus, the results can be broken down to the level of individual charging events and charging points. Among other factors, the study considers the expected growth in electric vehicles, the construction and operating costs for the investigated charging infrastructure, and the impact of heterogeneous electric vehicle fleets with different vehicle-specific charging powers. Since both technological and economic perspectives are considered in the framework, the study is relevant for all decision makers involved in the development and operation of charging infrastructure. The results in the investigated case of park and ride facilities show a high potential for cost-efficient low-power charging concepts. Thus, significantly higher energy volumes could be transmitted and better economic results could be achieved by the investigated low-power approaches. Especially for heterogeneous electric vehicle fleets, the number of available charging points appears to be more important than the charging power of the individual charging points in this case.

Managing forests for the supply of multiple ecosystem services (ES) is key given potential trade-offs among services. Here, the authors analyse how forest stand attributes generate trade-offs among ES and the relative contribution of forest attributes and environmental factors to predict services.

Ruxolitinib is a small-molecule protein kinase inhibitor, which is used as a therapeutic agent against several diseases. Due to its anti-inflammatory impact, ruxolitinib has also been considered recently for usage in the treatment of Covid-19. While the specific effects of ruxolitinib on Janus kinases (JAK) is comparatively well investigated, its (unspecific) impact on membranes has not been studied in detail so far. Therefore, we characterized the interaction of this drug with lipid membranes employing different biophysical approaches. Ruxolitinib incorporates into the glycerol region of lipid membranes causing an increase in disorder of the lipid chains. This binding, however, has only marginal influence on the structure and integrity of membranes as found by leakage and permeation assays.

Considering ongoing biodiversity losses, translocations of threatened plant species into the wild has become a common conservation technique although they are considered laborious, expensive and rarely successful. It is broadly accepted that the choice of suitable translocation sites and the translocation of many individuals increase their success. Moreover, high genetic diversity among introduced plants is suggested to be important but has been rarely assessed for populations of threatened species. Here, we tested whether higher genetic diversity generally increases early establishment of threatened plant species after a translocation. We translocated plantlets of eight rare and threatened species of Switzerland at one apparently suitable site per species. We planted 40 to 312 plants per site at two levels of genetic diversity, monoculture plots with offspring of single seed families per species, and mixture plots with offspring of several seed families per species. In the early translocation stages, plots with individuals of several seed families had a higher survival than plots with individuals of only one seed family, however, the positive effect of diversity disappeared with time. Our study suggests that a high genetic diversity is important at least for the short-term survival of translocated populations of threatened plant species. Translocations should therefore always aim to maximise genetic diversity in founder populations. Experimental approaches as the one we present here are important tools to realise long-term translocation experiments that are needed to enhance our understanding of the underlying factors responsible for success or failure of translocations of threatened plant species. We therefore recommend close collaborations between experimental plant ecologists, field botanists and conservation practitioners in future translocations of threatened species.

The monitoring of species and functional diversity is of increasing relevance for the development of strategies for the conservation and management of biodiversity. Therefore, reliable estimates of the performance of monitoring techniques across taxa become important. Using a unique dataset, this study investigates the potential of airborne LiDAR-derived variables characterizing vegetation structure as predictors for animal species richness at the southern slopes of Mount Kilimanjaro. To disentangle the structural LiDAR information from co-factors related to elevational vegetation zones, LiDAR-based models were compared to the predictive power of elevation models. 17 taxa and 4 feeding guilds were modeled and the standardized study design allowed for a comparison across the assemblages. Results show that most taxa (14) and feeding guilds (3) can be predicted best by elevation with normalized RMSE values but only for three of those taxa and two of those feeding guilds the difference to other models is significant. Generally, modeling performances between different models vary only slightly for each assemblage. For the remaining, structural information at most showed little additional contribution to the performance. In summary, LiDAR observations can be used for animal species prediction. However, the effort and cost of aerial surveys are not always in proportion with the prediction quality, especially when the species distribution follows zonal patterns, and elevation information yields similar results.

Nature’s contributions to people (NCPs) underpin the attainment of the Sustainable Development Goals (SDGs) but are declining globally. It is therefore critical to identify the drivers of changes in NCPs, and to understand how and where NCPs can contribute towards the achievement of the SDGs. By integrating the conceptual framework of the Intergovernmental Science-Policy Platform on Biodiversity and Ecosystem Services (IPBESs) and the SDGs, we can obtain a better understanding of how changes in the state of NCPs support or hinder attainment of the SDGs, and how changes in NCPs are driven by development interventions. We conducted a systematic synthesis of the literature to determine the state of research on NCPs, trends in NCPs and their drivers, and the contribution of NCPs towards achieving the SDGs in Nepal, a low-income and highly biodiverse country. We found that NCPs contributed positively towards the achievement of 12 SDGs. However, NCPs were reported to be declining across Nepal, ultimately undermining Nepal’s ability to achieve SDG targets. The major direct drivers of decline were land-use change, over-exploitation, and climate change. These direct drivers were linked to conventional development interventions, including agricultural expansion and the construction of road and energy infrastructure. However, some interventions, such as community forestry and protected areas, increased the supply of NCPs. Better integration of Indigenous knowledge and local practices was also reported to be effective in improving the provision of NCPs and contributing to improving livelihoods at local scales. We identified opportunities for further research in NCPs, particularly in increasing geographical representativeness and improving our understanding of non-material NCPs. Our approach of combining the IPBES conceptual framework and the SDGs enabled us to more comprehensively identify how progress towards the SDGs are mediated by NCPs and provides actionable guidelines for how to take more integrative measures to achieve the SDGs in Nepal and countries facing similar development challenges.

Mountains are facing growing environmental, social, and economic challenges. Accordingly, effective policies and management approaches are needed to safeguard their inhabitants, their ecosystems, their biodiversity, and the livelihoods they support. The formulation and implementation of such policies and approaches requires a thorough understanding of, and extensive knowledge about, the interactions between nature and people particular to mountain social–ecological systems. Here, we applied the conceptual framework of the Intergovernmental Science-Policy Platform on Biodiversity and Ecosystem Services to assess and compare the contents of 631 abstracts on the interactions among biodiversity, ecosystem services, human wellbeing, and drivers of change, and formulate a set of research recommendations. Our comparative assessment of literature pertained to the Andes, the East African mountains, the European Alps, and the Hindu Kush Himalaya. It revealed interesting differences between mountain systems, in particular in the relative importance given in the literature to individual drivers of change and to the ecosystem services delivered along elevational gradients. Based on our analysis and with reference to alternative conceptual frameworks of mountain social–ecological systems, we propose future research directions and options. In particular, we recommend improving biodiversity information, generating spatially explicit knowledge on ecosystem services, integrating knowledge and action along elevational gradients, generating knowledge on interacting effects of global change drivers, delivering knowledge that is relevant for transformative action toward sustainable mountain development, and using comprehensive concepts and codesigned approaches to effectively address knowledge gaps.

Abstract Increasing CO2 concentration ([CO2]) is likely to affect future species distributions, in interaction with other climate change drivers. However, current modeling approaches still seldom consider interactions between climatic factors and the importance of these interactions therefore remains mostly unexplored. Here, we combined dendrochronological and modeling approaches to study the interactive effects of increasing [CO2] and temperature on the distribution of one of the main European liana species, Hedera helix. We combined a classical continent‐wide species distribution modeling approach with a case study using H. helix and Quercus cerris tree rings, where we explored the long‐term influence of a variety of climate drivers, including increasing [CO2], and their interactions, on secondary growth. Finally, we explored how our findings could influence the model predictions. Climate‐only model predictions showed a small decrease in habitat suitability for H. helix in Europe; however, this was accompanied by a strong shift in the distribution toward the north and east. Our growth ring data suggested that H. helix can benefit from high [CO2] under warm conditions, more than its tree hosts, which showed a weaker response to [CO2] coupled with higher cavitation risk under high temperature. Increasing [CO2] might therefore offset the negative effects of high temperatures on H. helix, and we illustrate how this might translate into maintenance of H. helix in warmer areas. Our results highlight the need to consider carbon fertilization and interactions between climate variables in ecological modeling. Combining dendrochronological analyses with spatial distribution modeling may provide opportunities to refine predictions of how climate change will affect species distributions.

Differential responses of plant and animal functional diversity to climatic variation could affect trait matching in mutualistic interactions. Here, Albrecht et al. show that network structure varies across an elevational gradient owing to bottom-up and top-down effects of functional diversity.

Abstract Plant functional traits reflect individual and community ecological strategies. They allow the detection of directional changes in community dynamics and ecosystemic processes, being an additional tool to assess biodiversity than species richness. Analysis of functional patterns in plant communities provides mechanistic insight into biodiversity alterations due to anthropogenic activity. Although studies have consi‐dered of either anthropogenic management or nutrient availability on functional traits in temperate grasslands, studies combining effects of both drivers are scarce. Here, we assessed the impacts of management intensity (fertilization, mowing, grazing), nutrient stoichiometry (C, N, P, K), and vegetation composition on community‐weighted means (CWMs) and functional diversity (Rao's Q) from seven plant traits in 150 grasslands in three regions in Germany, using data of 6 years. Land use and nutrient stoichiometry accounted for larger proportions of model variance of CWM and Rao's Q than species richness and productivity. Grazing affected all analyzed trait groups; fertilization and mowing only impacted generative traits. Grazing was clearly associated with nutrient retention strategies, that is, investing in durable structures and production of fewer, less variable seed. Phenological variability was increased. Fertilization and mowing decreased seed number/mass variability, indicating competition‐related effects. Impacts of nutrient stoichiometry on trait syndromes varied. Nutrient limitation (large N:P, C:N ratios) promoted species with conservative strategies, that is, investment in durable plant structures rather than fast growth, fewer seed, and delayed flowering onset. In contrast to seed mass, leaf‐economics variability was reduced under P shortage. Species diversity was positively associated with the variability of generative traits. Synthesis. Here, land use, nutrient availability, species richness, and plant functional strategies have been shown to interact complexly, driving community composition, and vegetation responses to management intensity. We suggest that deeper understanding of underlying mechanisms shaping community assembly and biodiversity will require analyzing all these parameters.

For the untargeted analysis of the metabolome of biological samples with liquid chromatography–mass spectrometry (LC-MS), high-dimensional data sets containing many different metabolites are obtained. Since the utilization of these complex data is challenging, different machine learning approaches have been developed. Those methods are usually applied as black box classification tools, and detailed information about class differences that result from the complex interplay of the metabolites are not obtained. Here, we demonstrate that this information is accessible by the application of random forest (RF) approaches and especially by surrogate minimal depth (SMD) that is applied to metabolomics data for the first time. We show this by the selection of important features and the evaluation of their mutual impact on the multi-level classification of white asparagus regarding provenance and biological identity. SMD enables the identification of multiple features from the same metabolites and reveals meaningful biological relations, proving its high potential for the comprehensive utilization of high-dimensional metabolomics data.