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1. DNA-based molecular identification of Urnula mediterranea (Ascomycota, Pezizales) collected in Central Serbia

Author

Arsenijević Dejan D., Blagojević Stefan Z., Planojević Nevena S., Nikezić Aleksandra G., Vidanović Dejan S., Milosavljević Nenad, and Marković Snežana D.

Subjects
molecular systematics, dna markers, serbia, urnula mediterranea, Science
Abstract

Molecular systematics is a branch of systematics that analyzes genetic markers for the classification of organisms. Genetic markers include target sequences of molecules DNA, RNA, and amino acids. The most often used genetic markers are DNA markers, localized on DNA molecules. The study aim was DNA-based molecular identification of species Urnula mediterranea (M. Carbone, Agnello & Baglivo) M. Carbone, Agnello & P. Alvarado (2013), by using molecular systematics methods. This species was recently discovered for the first time in Central Serbia and determined only based on morphological characteristics. For the first time, U. mediterranea sampled in Serbia, was identified with DNA molecular markers. Extraction of DNA molecules was performed from the fruiting body of U. mediterranea. ITS region and the part of the gene for 28S rRNA were amplified and sequenced by the Sanger method. Comparing the analyzed sequences with the sequences from the database, a match of 99.72% was found for the ITS region and 100% for the part of the gene for 28S rRNA. By molecular identification, it has been established that the sample belongs to the species U. mediterranea. Both analyzed DNA sequences of U. mediterranea were deposited into the NCBI database.

Published
2021
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2. Comparative analysis of human DNA extraction methods and mitochondrial DNA HV1 and HV2 haplogroup determination

Author

Nikezić Aleksandra G., Blagojević Stefan Z., Ćupurdija Maja Đ., Planojević Nevena S., Jovankić Jovana V., Rakobradović Jelena D., Vidanović Dejan S., Arsenijević Dejan D., and Marković Snežana D.

Subjects
dna ancestry, dna isolation, hv1, hv2, mtdna haplogroups, Science
Abstract

The high integrity of extracted DNA is necessary for the determination of human haplogroups, based on the mitochondrial DNA (mtDNA) marker sequences. The study aims were to isolate the total DNA from selected human samples and establish a protocol that gives the best yield and proper purity of DNA material for further analysis. Furthermore, human hypervariable regions - HV1 and HV2, located on mtDNA were sequenced to define human haplogroups. For extraction, samples of buccal mucosa from ten human volunteers were taken and three different protocols were used (a method with ammonium acetate, a salting-out method and GeneJET Genomic DNA Purification Kit (Thermo Fisher Scientific)). Concentration and purity of DNA were measured on BioPhotometer. Sequencing was performed by Sanger method on 3130 Genetic Analyzer (Applied Biosystems) with commercial BigDye™ Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems). Sequences were processed using Chromas and MEGA software and compared with sequence databases with BLAST tool on NCBI. Haplogroups and mutations were determined in the MITOMAP online database. Results of extraction indicated that the Purification Kit gives the highest concentration of DNA (11.86±0.63) and low purity (1.17±0.02), while a method with ammonium acetate gave the best purity (1.67±0.17). Sequencing obtained on DNA extracted with a commercial kit, gave 5 different human haplogroups U2e, T1a, J1c, H2a, K. In conclusion, it is recommended to use the Purification Kit for obtaining high integrity DNA that can be used for effective sequencing. Determination of haplogroups and haplotypes can reveal our ancestry and have important implications in molecular systematics, phylogenetics and phylogeography.

Published
2020

3. Bioinformatics online support for bioactive substances cytotoxicity testing and their statistical analysis

Author

Blagojević Stefan Z., Cvjetković Vladimir M., Nikezić Aleksandra G., Milutinović Milena G., and Marković Snežana D.

Subjects
anova, cancer cell lines, lcmb ic50 database, remote access, Science
Abstract

Preclinical in vitro/in vivo testing is the first step in discovery of anticancer medicines, among others evaluation of cytotoxic activity of bioactive substances (BAS) on various human normal and cancer cell lines. Cytotoxicity expressed as IC50 value (a dose that inhibits 50% of cell growth) is one of the most commonly used parameter for comparable analysis of activity between different substances. This study includes examination of number of BAS and their results for cytotoxic activity obtained in the Laboratory for cell and molecular biology (LCMB) that require various statistical and computational techniques for proper effective analysis. In order to improve experimental data analysis, make it faster, more effective, error proof with secure online data repository, a Web application with LCMB IC50 database was developed as a useful research tool acting as a leverage for scientific data processing requirements. Analysis includes cytotoxic effects of chemical and natural BAS (IC50 values) on HCT-116, SW-480, MDA-MB-231, and MRC-5 cell lines. Generally, it can be concluded that BAS of different origin, chemical or natural, have various cytotoxic effects and cause different cell line sensitivity, which is presented and discussed. This paper presents developed SQL database-centric Web application with remote user-friendly data management for a biology researcher user type profile. Data processing in this article can be useful for a further overlook and testing of cytotoxic substances.

Published
2020

4. Altered apoptosis and biotransformation signaling in HCT-116 colorectal carcinoma cells induced by Teucrium chamaedrys L. extract

Author

Milutinović Milena G., Nikodijević Danijela D., Stanković Milan S., Cvetković Danijela M., and Marković Snežana D.

Subjects
anticancer properties, apoptosis, biotransformation, food supplement, plant extract, Teucrium, Science
Abstract

The aim of this study was an investigation of pro-apoptotic activity of methanol extract from T. chamaedrys, a more detailed determination of the signal molecules activated in the process of apoptosis, and effects on mRNA expression of enzymes involved in biotransformation (CYP1A1 and GSTP1) and membrane transporter, MRP-2 in HCT-116 colorectal carcinoma cells. The results show pronounced proapoptotic activity of T. chamaedrys extract, due to activation of both extrinsic and intrinsic pathways. The death receptor associated signaling pathway was activated in HCT-116 following treatment by T. chamaedrys, via increased Fas receptor expression and activity of caspase 8. Activation of caspase 9 suggests that mitochondrial signalling also has an impact. The extract reduced mRNA expression of GSTP1 and MRP-2 genes, as one of the causes of multi drug resistance in cancer cells. Observed results offer the possibility for the use of T. chamaedrys extract in the context of cancer prevention and therapy.

Published
2019

5. The concentration of matrix metalloproteinase 9 in the tumor and peritumoral tissue as prognostic marker in breast cancer patients

Author

Cvetković Danijela M., Milošević Bojan Z., Cvetković Aleksandar M., Ninković Srđan M., Jovankić Jovana V., Jovanović Dalibor V., and Marković Snežana D.

Subjects
breast neoplasms, disease progression, matrix metalloproteinase 9, risk assessment, tissues, Medicine (General), R5-920
Abstract

Background/Aim. Breast cancer is one of the most common malignancies among women all over the world. Tumor microenvironment represents one of the main regulators of tumorigenesis. We investigated the role of matrix metalloproteinases 9 (MMP-9) concentration in peritumoral tissue as a prognostic marker in the breast cancer patients. Methods. The ELISA test was used to determine a total MMP-9 concentration in carcinoma and peritumoral tissue sample in the patients with breast cancer. Comparison of MMP-9 protein expression with the clinicopathological parameters was evaluated. Results. Peritumoral tissue at 3 cm distance from the tumor produces more MMP-9 than the tumor itself. The ratio of concentrations of MMP-9 in the tumor and peritumoral tissue considerably changes in favor of peritumoral tissue with the increase of tumor size and the involvement of axillary lymph nodes. In N0 stage, the concentration ratio of MMP-9 in the tumor and peritumoral tissues was 1 : 1.44, but in the N2 stage, the ratio was 1 : 26.5. Conclusion. In patients with breast cancer even in an early stadium there is a change in MMP-9 concentration in peritumoral tissue. We can extract the group of patients at increased risk for the development of lymph node metastasis. A statistically significant difference between the concentrations of MMP-9 in the peritumoral tissue and cancer tissue exists only in case of metastatic disease not in MO stadium implying need for early detection of still unknown metastases in such patients. [Projects of the Serbian Ministry of Education, Science and Technological Development, Grant no. III41010 and Grant no. III41007]

Published
2019
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6. Effects of royal jelly on energy status and expression of apoptosis and biotransformation genes in normal fibroblast and colon cancer cells

Author

Jovanović Milena M., Ćupurdija Maja Đ., Nikodijević Danijela D., Milutinović Milena G., Cvetković Danijela M., Rakobradović Jelena D., and Marković Snežana D.

Subjects
apoptosis, LDH, royal jelly, superoxide anion radical, ATP, colorectal cancer, Science
Abstract

Royal jelly is natural bee product, traditionally used in medicine for antitumor, anti-inflammatory, antioxidant, antibiotic and many other beneficial properties. The aim of this study was to determine biological effects of royal jelly samples originating from Serbia on normal human fibroblast (MRC-5) and colorectal cancer (HCT-116 and SW-480) cells. MTT cell viability assay was used to determine cytotoxic activity, and NBT test was used for determination of superoxide anion radical concentration. Parameters of cell energy status were determined using LDH and ATP colorimetric methods. Relative expression of mRNA of apoptosis and biotransformation genes was monitored by qPCR method. Royal jelly affected cell viability, caused oxidative stress appearance and elevated parameters of energy status in cancer cell lines. The relative expression of genes whose proteins are included in biotransformation of xenobiotics were changed with notable suppression of CYP1A1, while increased expression of apoptosis genes was noted in tested cell lines. Royal jelly demonstrated cell selective effect and could be prospective in anticancer therapy.

Published
2018

7. Cytotoxic, antimigratory, pro-and antioxidative activities of extracts from medicinal mushrooms on colon cancer cell lines

Author

Šeklić Dragana S., Stanković Milan S., Milutinović Milena G., Topuzović Marina D., Štajn Andraš Š., and Marković Snežana D.

Subjects
colon cancer, cytotoxicity, migration, mushrooms extracts, oxidative stress, Biology (General), QH301-705.5
Abstract

Methanol extracts of five commercially available mushroom species (Phellinus linteus (Berk. et Curt) Teng, Cordyceps sinensis (Berk.) Sacc., Lentinus edodes (Berk.) Pegler, Coprinus comatus (O. F. Müll.) Pers. and Ganoderma lucidum (Curtis) P. Karst), traditionally used as anticancer agents, were evaluated in vitro for their total phenol and flavonoid contents, cytotoxic and antimigratory activities and antioxidant/prooxidant effects on colon cancer cell lines (HCT-116 and SW-480). Spectrophotometric methods were used for the determination of total phenol content, flavonoid concentrations and DPPH activity of the extracts. Cytotoxic activity was measured by the MTT assay. The antimigratory activity of extracts was determined using the Transwell assay and immunofluorescence staining of β-catenin. The prooxidant/antioxidant status was followed by measuring the superoxide anion radical (O2•-), nitrite and reduced glutathione (GSH) concentrations. Our results show that the highest phenolic and flavonoid content was found in P. linteus, and its DPPH-scavenging capacity was significantly higher than in other samples. The P. linteus extract significantly decreased cell viability of both tested cancer cell lines. All other extracts selectively inhibited SW-480 cell viability, but did not show significant cytotoxic activity. The mushroom extracts caused changes in the prooxidant/antioxidant status of cells, inducing oxidative stress. All extracts tested on HCT-116 cells demonstrated significant antimigratory effects, which correlated with increased production of O2•- and a reduced level of β-catenin protein expression, while only P. linteus showed the same effect on SW-480 cells. The results of the present research indicate that the mushroom extracts causes oxidative stress which has a pronounced impact on the migratory status of colon cancer cell lines. [Projekat Ministarstva nauke Republike Srbije, br. III41010]

Published
2016
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8. Effects of Teucrium spp.: Extracts on migratory potential and redox status of human colon SW-480 and breast MDA-MB-231 cancer cells

Author

Živanović Marko N., Stojanović Aleksandra Z., Cvetković Danijela M., Milutinović Milena G., Stanković Milan S., and Marković Snežana D.

Subjects
breast cancer, colon cancer, migration, redox status, Teucrium spp., Science
Abstract

Plant extracts are proved to possess many positive effects in cancer treatment. While many of commonly used therapeutics rely on cytotoxic methodologies, much less are based on positive influence on migrating potential of cancer cells, i.e. on antimetastatic grounds. Here, according to our knowledge, for the first time was investigated influence of Teucrium montanum L. and T. polium L. methanolic extracts on migratory potential of colorectal cancer cell line SW-480 and human breast carcinoma MDA-MB-231 cell line. The redox status was evaluated on the basis of concentration of superoxide anion radical (O2 .-), nitrites and reduced glutathione (GSH), determined spectrophotometrically, while migratory potential of cancer cells was followed by real time cell analysis. Our results showed acute increasing of superoxide anion radical, decreasing of nitrites and decreasing of GSH in SW-480 cells. In MDA-MB-231 cells investigated extracts showed no change in GSH level. Also, methanol extract of T. montanum in low concentrations decreased cell migration, while T. polium showed antimigratory potential in higher concentrations and in treatment periods longer than 12 hours. Investigated Teucrium spp. extracts showed changes in redox status leading to enhancement of oxidative stress. Also, results indicated moderate reducing of migratory potential of tested cancer cells. However, correlation between redox status and antimigratory effects were not observed. According to these findings, the additional investigation of the exact mechanism of antimigratory effect should be performed.

Published
2016
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9. Antioxidant and anticancer properties of leaves and seed cones from European yew (Taxus baccata L.)

Author

Milutinović Milena G., Stanković Milan S., Cvetković Danijela M., Topuzović Marina D., Mihailović Vladimir B., and Marković Snežana D.

Subjects
antioxidant activity, apoptosis, cytotoxity, Taxus baccata L., Biology (General), QH301-705.5
Abstract

Plant extracts of the leaves and seed cones of European yew Taxus baccata L. (Taxaceae) were analyzed for total phenolic content, flavonoid concentrations, antioxidant and anticancer properties (cytotoxic and proapoptotic activity). The total phenolic content ranged between 8.23 and 210.01 mg Ga/g, with the IC50 values for antioxidant activity between 25.24 and 533.66 μg/ml. The MTT test showed that the methanolic extract of leaves had better activity on HCT-116 cells than the extract of seed cones, with IC50 values of 14.3 for 24 h and 4.59 for 72 h. The MDA-MB-231 cell line displayed significantly lower sensitivity to both extracts as compared to the HCT-116 cell line. Microscopic examination indicated that the extracts induced apoptosis in both cell lines. These results suggest that T. baccata leaves and seed cones are a potential source of phenolic compounds, especially flavonoids, as natural antioxidant, cytotoxic and strong proapoptotic substances of high value.

Published
2015
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10. Alteration of oxidative stress parameters in red blood cells of rats after chronic in vivo treatment with cisplatin and selenium

Author

Marković Snežana D., Žižić Jovana B., Đačić Dragana S., Obradović Ana D., Ćurčić Milena G., Cvetković Danijela M., Đorđević Nataša Z., Ognjanović Branka I., and Štajn A.Š.

Subjects
Antioxidative defense system, cisplatin, hematology, oxidative stress, selenium, Biology (General), QH301-705.5
Abstract

In this study we evaluated the possible protective effects of selenium (Se) on hematological and oxidative stress parameters in rats chronically treated with cisplatin (cisPt). Four groups of Wistar albino rats were examined: a control, untreated rats (I), rats treated with Se (II), rats treated with cisPt (III), and rats treated with Se and cisPt (IV). All animals were treated for 5 days successively and killed 24 h after the last treatment. Hematological and oxidative stress parameters were followed in whole blood and red blood cells (RBC). Results showed that the chronic application of Se was followed by a higher number of reticulocytes and platelets, increased lipid peroxidation and GSH content in the RBC. Cisplatin treatment induced depletion of RBC and platelet numbers and an elevation of the superoxide anion, nitrites and glutathione levels. Se and cisPt co-treatment was followed by an elevation of the hematological parameters and the recovery of the glutathione status when compared to the control and cisPt-treated rats.

Published
2011
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11. Glutathione status in the blood of rats after reticulocytosis induced by phenylhydrazine and bleeding

Author

Marković Snežana D., Obradović Ana D., Žižić Jovana B., Ognjanović Branka I., Štajn A.Š., Saičić Zorica S., and Spasić M.B.

Subjects
bleeding, blood, glutathione, phenylhydrazine, rats, Biology (General), QH301-705.5
Abstract

In this experiment, we compared the in vivo effects of phenylhydrazine (PHZ) and bleeding treatment on the redox status and glutathione antioxidative mechanism parameters in the plasma and red blood cells (RBC) of rats. Results showed a lower level of reactive oxygen species (ROS), a higher level of lipid peroxidation and the effective antioxidative role of the glutathione system in the blood of bleeding rats. PHZ-treatment induced higher concentrations of ROS and an accumulation of oxidized glutathione in the plasma, while the glutathione system showed a satisfactory antioxidative capacity in the RBC of rats. When comparing the two anemic groups, the PHZ-treated rats showed marked oxidative stress in the plasma. .

Published
2010
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12. Effects of cisplatin on lipid peroxidation and the glutathione redox status in the liver of male rats: The protective role of selenium

Author

Trbojević Ivana S., Ognjanović Branka I., Đorđević Nataša Z., Marković Snežana D., Štajn A.Š., Gavrić Jelena P., and Saičić Zorica S.

Subjects
Cisplatin, selenium, lipid peroxidation, glutathione redox status, liver, rat, Biology (General), QH301-705.5
Abstract

The role of oxidative stress in cisplatin (CP) toxicity and its prevention by pretreatment with selenium (Se) was investigated. Male Wistar albino rats were injected with a single dose of cisplatin (7.5 mg CP/kg b.m., i.p.) and selenium (6 mg Se/kg b.m, as Na2SeO3, i.p.) alone or in combination. The results suggest that CP intoxication induces oxidative stress and alters the glutathione redox status: reduced glutathione (GSH), oxidized glutathione (GSSG) and the GSH/GSSG ratio (GSH RI), resulting in increased lipid peroxidation (LPO) in rat liver. The pretreatment with selenium prior to CP treatment showed a protective effect against the toxic influence of CP on peroxidation of the membrane lipids and an altering of the glutathione redox status in the liver of rats. From our results we conclude that selenium functions as a potent antioxidant and suggest that it can control CP-induced hepatotoxicity in rats.

Published
2010
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13. Time course of hematological parameters in bleeding-induced anemia

Author

Marković Snežana D., Milošević Marijana P., Đorđević Nataša Z., Ognjanović Branka I., Štajn A.Š., Saičić Zorica S., and Spasić M.B.

Subjects
anemia, bleeding, hematological parameters, rats, Biology (General), QH301-705.5
Abstract

In order to investigate daily changes of hematological parameters in bleeding-induced anemia, we treated Wistar albino male rats by daily bleeding (1.5-2 mL of blood from the tail vein for eight days). Blood samples were taken before (on day zero) and on the first to eighth days of bleeding. The values of hematocrit, hemoglobin, and erythrocyte count decreased significantly after the second, sixth, and second days of bleeding, respectively. The number of leuko­cytes and platelets, as well as Heinz body levels, increased significantly after the third and second days of treatment. The percentage of reticulocytes increased significantly from the second day and attained the maximum level (32.55 ± 0.96%) on the eighth day.

Published
2009
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18. Stereospecific ligands and their complexes. Part XII. Synthesis, characterization and in vitro antiproliferative activity of platinum(IV) complexes with some O,O′-dialkyl esters of (S,S)-ethylenediamine-N,N′-di-2-propanoic acid against colon cancer (HCT-116) and breast cancer (MDA-MB-231) cell lines

Author

Stojković, Danijela Lj., Jevtić, Verica V., Radić, Gordana P., Đačić, Dragana S., Ćurčić, Milena G., Marković, Snežana D., Ðinović, Vesna M., Petrović, Vladimir P., and Trifunović, Srećko R.

Published
2014
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20. Hygrophorus eburneus, edible mushroom, a promising natural bioactive agent

Author

Kosanić, Marijana M., Šeklić, Dragana S., Jovanović, Milena M., Petrović, Nevena N., and Marković, Snežana D.

Subjects
antioxidant, edible mushroom, antimicrobial, Original Article, neuroprotective, anticancer
Abstract

It is known that many edible mushrooms have important medicinal properties, including effects on different types of cancers. This is the first report regarding the neuroprotective, antimicrobial, antioxidative and anticancer activities of the acetone extract of edible mushroom Hygrophorus eburneus. Neuroprotective potential was evaluated by measuring the capacity of the extract to inhibit acetylcholinesterase. In this assay, the tested extract showed activity against acetylcholinesterase in a dose-dependent manner where the percentage of inhibition ranged from 13.19 to 46.44 %. The antimicrobial potential was determined by the microdilution method against five species of bacteria and eight species of fungi and the results of this method exhibited moderate antimicrobial activity of H. eburneus with MIC values ranging from 6.25 to 25 mg/mL. Antioxidant activity was evaluated by measuring the scavenging capacity of the tested sample on DPPH and superoxide anion radicals, by the reducing power assay and by measuring the amounts of total phenolics in extract. As a result of the study, H. eburneus extract showed a potent antioxidant activity (IC50 were 102.93 μg/mL for DPPH radical scavenging activity and 123.27 μg/mL for superoxide anion radicals scavenging) while absorbances for reducing power assay were from 0.0235 to 0.1161. The total phenolic content in the extract was 9.27 µg PE/mg. Finally, anticancer effects were evaluated by MTT test for cytotoxicity, acridine orange/ethidium bromide staining for detection of the type of cell death and wound healing assay for antimigratory effects on human colorectal cancer cell line (HCT-116) and human breast cancer cell line (MDA-MB-231). The results for cytotoxicity and apoptosis were measured after 24 and 72 h and for anti-migratory effect after 12 and 24 h. The tested H. eburneus mushroom extract expressed cell selectivity, with notable cytotoxic effects observed on HCT-116 cells, with a strong proapoptotic potential. The migration of HCT-116 cells was significantly inhibited, while MDA-MB-231 cells were less sensitive to the treatment. The results of this study revealed that the tested extract had relatively strong neuroprotective, antimicrobial, antioxidant, and anticancer effects. It suggests that this mushroom can be proposed as a novel source of nutraceuticals and pharmaceuticals., EXCLI Journal;Vol. 19 2020

Published
2020

29. Impact of bee venom and melittin on apoptosis and biotransformation in colorectal carcinoma cell lines

Author

Nikodijević, Danijela D., Milutinović, Milena G., Cvetković, Danijela M., Ćupurdija, Maja Đ., Jovanović, Milena M., Mrkić, Ivan V., Gavrović-Jankulović, Marija, Marković, Snežana D., Nikodijević, Danijela D., Milutinović, Milena G., Cvetković, Danijela M., Ćupurdija, Maja Đ., Jovanović, Milena M., Mrkić, Ivan V., Gavrović-Jankulović, Marija, and Marković, Snežana D.

Abstract

This study provides data about anticancer properties of bee venom and its dominant compound, melittin on colorectal carcinoma cells (HCT-116 and SW-480) in regard to their proapoptotic activity and expression of genes involved in biotransformation process. Based on results, they are strong cytotoxins, where the melittin showed also selectivity against cancer cells compared to normal, HaCat. They induce proapoptotic activity by affecting apoptosis signaling molecules (Fas receptors, caspase 9, and members of Bcl-2 family of proteins) and mainly suppress expression of genes involved in their biotransformation, suggesting their ability to develop the resistance of colorectal cancer cells.

Published
2021

30. Supplementary data for the article: Nikodijević, D. D.; Milutinović, M. G.; Cvetković, D. M.; Ćupurdija, M. Đ.; Jovanović, M. M.; Mrkić, I. V.; Jankulović-Gavrović, M. Đ.; Marković, S. D. Impact of Bee Venom and Melittin on Apoptosis and Biotransformation in Colorectal Carcinoma Cell Lines. Toxin Reviews 2021, 40 (4), 1272–1279. https://doi.org/10.1080/15569543.2019.1680564.

Author

Nikodijević, Danijela D., Milutinović, Milena G., Cvetković, Danijela M., Ćupurdija, Maja Đ., Jovanović, Milena M., Mrkić, Ivan V., Gavrović-Jankulović, Marija, Marković, Snežana D., Nikodijević, Danijela D., Milutinović, Milena G., Cvetković, Danijela M., Ćupurdija, Maja Đ., Jovanović, Milena M., Mrkić, Ivan V., Gavrović-Jankulović, Marija, and Marković, Snežana D.

Published
2021

32. Proapoptotic and Antimigratory Effects of Pseudevernia furfuracea and Platismatia glauca on Colon Cancer Cell Lines

Author

Šeklić, Dragana S., Obradović, Ana D., Stanković, Milan S., Živanović, Marko N., Mitrović, Tatjana Lj., Stamenković, Slaviša M., and Marković, Snežana D.

Subjects
0301 basic medicine, Pseudevernia furfuracea, General Chemical Engineering, lcsh:Biotechnology, redox status, colorectal cancer, cell totility, cell motility, Industrial and Manufacturing Engineering, 03 medical and health sciences, chemistry.chemical_compound, lichen extracts, 0302 clinical medicine, lcsh:TP248.13-248.65, Cytotoxic T cell, Viability assay, Original Scientific Papers, Cytotoxicity, biology, lcsh:TP368-456, Chemistry, Acridine orange, apoptosis, biology.organism_classification, Molecular biology, 3. Good health, cytotoxic effects, lcsh:Food processing and manufacture, 030104 developmental biology, Apoptosis, Cell culture, 030220 oncology & carcinogenesis, Cancer cell, Food Science, Biotechnology
Abstract

SUMMARY The aim of this study is to investigate cytotoxic, proapoptotic, antimigratory and pro-antioxidant effects of methanol, acetone and ethyl acetate extracts of lichens Pseudevernia furfuracea and Platismatia glauca on colorectal cancer (HCT-116 and SW-480) cell lines. We compared the cytotoxic effects on colorectal cancer cells with the effects obtained from normal human fibroblast (MRC-5) cell line. Tetrazolium (MTT) test evaluated the cytotoxic effects, Transwell assay evaluated cell migration, acridine orange/ethidium bromide (AO/EB) fluorescent method followed the apoptosis, while prooxidant/antioxidant effects were determined spectrophotometrically through concentration of redox parameters. The tested extracts showed considerable cytotoxic effect on cancer cells with no observable cytotoxic effect on normal cells. Ethyl acetate and acetone extract of P. furfuracea induced the highest cytotoxicity (IC50=(21.2±1.3) µg/mL on HCT-116, and IC50=(51.3±0.8) µg/mL on SW-480 cells, respectively, after 72 h), with noteworthy apoptotic and prooxidant effects, and antimigratory potential of methanol extract. P. glauca extracts induced cytotoxic effects on HCT-116 cells after 72 h (IC50

Published
2018

36. Synthesis, characterization, DFT study, DNA/BSA-binding affinity, and cytotoxicity of some dinuclear and trinuclear gold(III) complexes

Author

Radisavljević, Snežana, Ćoćić, Dušan, Jovanović, Snežana, Šmit, Biljana, Petković, Marijana, Milivojević, Nevena, Planojević, Nevena, Marković, Snežana D., Petrović, Biljana V., Radisavljević, Snežana, Ćoćić, Dušan, Jovanović, Snežana, Šmit, Biljana, Petković, Marijana, Milivojević, Nevena, Planojević, Nevena, Marković, Snežana D., and Petrović, Biljana V.

Abstract

In this study, we have synthesized a series of dinuclear and trinuclear gold(III) complexes of the general formula [Au2(N–N)Cl6] (1–3) for dinuclear and [Au3(N–N)2Cl8]+ (4–6) for trinuclear compounds, respectively, in which N–N is a bidentate ligand (1,4-diaminobutane; 1,6-diaminohexane or 1,8-diaminooctane). These complexes were characterized by elemental analysis, molar conductivity, and spectroscopic techniques (IR, UV–Vis, 1H NMR, ESI–MS). We performed DFT calculations to get insight into the geometry of the studies complexes. DNA-binding studies were performed by UV–Vis spectrophotometry and fluorescence spectroscopy. The results of competitive reactions between gold(III) complexes and ethidium bromide (EB) towards DNA have shown that selected complexes can displace EB from DNA-EB adduct. In addition, these experiments confirm that polynuclear gold(III) complexes interact with DNA covalently or via intercalation. Furthermore, high values of binding constants of gold(III) complexes towards bovine serum albumin (BSA) protein indicate good binding affinity. In addition, redox stability of complexes in the presence of DNA/BSA was confirmed by cyclic voltammetry. Results of the interactions between gold(III) complexes with DNA/BSA were discussed in reference to molecular docking data obtain by Molegro virtual docker. The cytotoxic activity of synthesized gold(III) complexes was evaluated on human breast cancer cell line (MDA-MB-231), human colorectal cancer cell line (HCT-116), and normal human lung fibroblast cell line (MRC-5). All complexes dose-dependently reduced cancer and normal cells viabilities, with significant cytotoxic effects (IC50 < 25 μM) for trinuclear gold(III) complexes (4, 5) on HCT-116 cells. Graphic abstract: [Figure not available: see fulltext.].

Published
2019

37. Potential of Teucrium chamaedrys L. to modulate apoptosis and biotransformation in colorectal carcinoma cells

Author

Milutinović, Milena, Maksimović, Vuk, Cvetković, Danijela M., Nikodijević, Danijela D., Stanković, Milan, Pesic, Milica, Marković, Snežana D., Milutinović, Milena, Maksimović, Vuk, Cvetković, Danijela M., Nikodijević, Danijela D., Stanković, Milan, Pesic, Milica, and Marković, Snežana D.

Abstract

Ethnopharmacological relevance: Teucrum chamaedrys L. is one of the known medicinal plants, useful for treatment of various health problems, especially digestive. In this study, we investigated methanol, ethyl-acetate and acetone extracts of T. chamaedrys in respect to their anticancer properties in SW480 colorectal cancer cells. Materials and methods: Cytotoxicity and proapoptotic potential were assessed by MTT cell viability assay and AO/EB double staining. Molecular mechanisms of induced apoptosis were determined by monitoring Fas receptor protein expression through immunofluorescence, Caspase 8 and 9 activity, as well as concentrations of O-2(center dot-) spectrophotometrically. Additionally, mRNA expression of biotransformation enzymes (CYP1A1, CYP1B1, GSTP1) and membrane transporters (MRP1 and MRP2) involved in drug resistance were investigated by qPCR method. Qualitative analysis of individual phenolic compounds was performed by reversed phase HPLC-MS analysis. Results: Methanol extract shows the best cytotoxicity and selectivity compared to ethyl-acetate and acetone extracts, mainly causing apoptosis of SW480 cells, without affecting normal HaCaT keratinocytes. The increased expression of Fas receptor protein and caspase 8 activity indicate that the death receptor-mediated pathway plays a crucial role in the observed apoptosis. The increased caspase 9 activity and O-2(center dot-) concentration suggest that mitochondria are also involved in the apoptosis. T. chamaedrys methanol extract inhibits mRNA expression of CYP1A1, CYPIBI, GSTP1, MRP1 and MRP2 in SW480 cells. Conclusions: Induction of apoptosis and inhibition of CYP1A1, CYP1B1, GSTP1, MRP1 and MRP2 mRNA expression implies that T. chamaedrys can serve as a valuable source of bioactive compounds as dietary supplements or selective anticancer agents, with the ability to induce apoptosis and modulate drug resistance in colorectal cancer cells.

Published
2019

41. Chemical composition, cytotoxic and antioxidative activities of ethanolic extracts of propolis on HCT-116 cell line

Author

Žižić, Jovana B., Vuković, Nenad L., Jadranin, Milka, Anđelković, Boban D., Tešević, Vele, Kacaniova, Miroslava M., Sukdolak, Slobodan B., and Marković, Snežana D.

Subjects
propolis, antioxidants, HCT-116 cell line, cytotoxicity, polyphenols
Abstract

BACKGROUND Propolis is a complex resinous sticky substance that honeybees collect from buds and exudates of various plants. Owing to its versatile biological and pharmacological activities, propolis is widely used in medicines, cosmetics and foods. The aim of this study was to evaluate the cytotoxic and antioxidative effects of various ethanolic extracts of propolis (EEPs) on human colon cancer cell line HCT-116 and compare them with their composition determined by HPLC-DAD. RESULTS The most abundant flavonoids in all samples were chrysin, pinocembrin and galangin (12.697-40.811 mu gmg(-1)), while the main phenolic acids were caffeic acid, ferulic acid and isoferulic acid. Dose- and time-dependent inhibition of growth of HCT-116 cells was observed for all propolis samples, with IC50 values ranging from 26.33 to 143.09 mu gmL(-1). Differences in cytotoxic activity of propolis samples were associated with differences in their composition. All EEP samples reduced both superoxide anion radical and nitrite levels and also had strong DPPH-scavenging activity. CONCLUSION All tested propolis samples had pronounced cytotoxic and antioxidative activities. This is the peer-reviewed version of the following article: Žižić, J. B.; Vuković, N. L.; Jadranin, M.; Anđelković, B. D.; Tešević, V.; Kacaniova, M. M.; Sukdolak, S. B.; Markovic, S. D. Chemical Composition, Cytotoxic and Antioxidative Activities of Ethanolic Extracts of Propolis on HCT-116 Cell Line. Journal of the Science of Food and Agriculture 2013, 93 (12), 3001–3009. [https://doi.org/10.1002/jsfa.6132] [http://cer.ihtm.bg.ac.rs/handle/123456789/1213]

Published
2013

42. Effects of acute treatment of vitamin C on redox and antioxidative metabolism in plasma and red blood cells of rats

Author

Marković, Snežana D., Đačić, Dragana S., Cvetković, Danijela M., Obradović, Ana D., Žižić, Jovana B., Ognjanović, Branka I., Štajn, Andraš Š., Saičić, Zorica, and Spasić, Mihajlo

Subjects
rats, vitamin C, oxidative stress, red blood cells
Abstract

Ascorbic acid (Vit C) is a reducing agent that efficiently quenches potentially damaging free radicals. The aim of this study was to investigate effects of high acute dose of Vit C on redox and antioxidative status in blood of rats. Our study included two groups of Wistar albino rats: I - untreated group (control); II - group treated with Vit C (500 mg/kg bw, i.p.). In all cases animals were sacrificed after three days of treatment and blood samples were taken at the day of sacrifice. Concentration of Heinz bodies (HB) was determined in whole blood, while the concentration of reactive oxygen and nitrogen species (ROS and RNS), as well as, parameters of antioxidative defense system were followed in plasma and red blood cells (RBC). Our results showed that Vit C treatment induced higher levels of peroxynitrites and lipid peroxidation in plasma. In RBC, Vit C induced higher level of HB, GSSG/2 GSH ratio and glutathione peroxidase activity, while decreased glutathione reductase activity. In conclusion, acute high doses of Vit C act as prooxidant and induced oxidative stress that resulted in lipid peroxidation in plasma and hemoglobin oxidation in RBC. . Projekat ministarstva br. 143035B

Published
2010

43. Efekti cisplatina na lipidnu peroksidaciju i glutation redoks status u jetri pacova - zaštitna uloga selena

Author

Trbojević, Ivana S., Ognjanović, Branka I., Đorđević, Nataša Z., Marković, Snežana D., Štajn, A.Š., Gavrić, Jelena, and Saičić, Zorica

Subjects
glutathione redox status, lipid peroxidation, rat, Cisplatin, selenium, liver
Abstract

The role of oxidative stress in cisplatin (CP) toxicity and its prevention by pretreatment with selenium (Se) was investigated. Male Wistar albino rats were injected with a single dose of cisplatin (7.5 mg CP/kg b.m., i.p.) and selenium (6 mg Se/kg b.m, as Na2SeO3, i.p.) alone or in combination. The results suggest that CP intoxication induces oxidative stress and alters the glutathione redox status: reduced glutathione (GSH), oxidized glutathione (GSSG) and the GSH/GSSG ratio (GSH RI), resulting in increased lipid peroxidation (LPO) in rat liver. The pretreatment with selenium prior to CP treatment showed a protective effect against the toxic influence of CP on peroxidation of the membrane lipids and an altering of the glutathione redox status in the liver of rats. From our results we conclude that selenium functions as a potent antioxidant and suggest that it can control CP-induced hepatotoxicity in rats. U našoj studiji ispitivana je uloga oksidacionog stresa u toksičnosti cisplatina (SR) i njegova prevencija pretretmanom selenom (Se). Mužjaci Wistar albino pacova su inicirani jednom dozom cisplatina (7.5 mg CP/kg t.m., i.p.) i selena (6 mg Se/kg t.m, kao Na2SeO3, i.p.) pojedinačno ili u kombinaciji. Rezultati pokazuju da intoksikacija SR uzrokuje oksidacioni stres i promenu glutation redoks statusa: redukovanog (GSH), oksidovanog (GSSG) i GSH/GSSG indeksa (GSH RI), kao i povećanje lipidne peroksidacije (LPO) u jetri pacova. Tretman Se koji je prethodio tretmanu SR pokazao je zaštitne efekte protiv toksičnog delovanja SR na peroksidaciju lipida membrane i promenu glutation redoks statusa u jetri pacova. Na osnovu naših rezultata zaključujemo da Se, deluje kao snažan antioksidans i da može imati ulogu u kontroli SR indukovane hepatotoksičnosti kod pacova. Projekat ministarstva br. 143035B

Published
2010

44. Glutationski status u krvi pacova nakon retikulocitoze izazvane fenilhidrazinom i krvarenjem

Author

Marković, Snežana D., Obradović, Ana D., Žižić, Jovana B., Ognjanović, Branka I., Štajn, A.Š., Saičić, Zorica, and Spasić, Mihajlo

Subjects
rats, blood, glutathione, bleeding, phenylhydrazine
Abstract

In this experiment, we compared the in vivo effects of phenylhydrazine (PHZ) and bleeding treatment on the redox status and glutathione antioxidative mechanism parameters in the plasma and red blood cells (RBC) of rats. Results showed a lower level of reactive oxygen species (ROS), a higher level of lipid peroxidation and the effective antioxidative role of the glutathione system in the blood of bleeding rats. PHZ-treatment induced higher concentrations of ROS and an accumulation of oxidized glutathione in the plasma, while the glutathione system showed a satisfactory antioxidative capacity in the RBC of rats. When comparing the two anemic groups, the PHZ-treated rats showed marked oxidative stress in the plasma. Cilj ovog rada je bio da se isprate in vivo efekti fenilhidrazinom (PHZ) i krvarenjem indukovane retikulocitoze na parametre redoks i glutationskog antioksidativnog statusa u plazmi i crvenim krvnim ćelijama (RBC) pacova. Rezultati pokazuju niži nivo reaktivnih vrsta kiseonika (ROS), viši nivo lipidne peroksidacije i efikasnu antioksidacionu ulogu glutationskog sistema u krvi iskvavljenih pacova. Tretman PHZom prouzrokovao je više koncentracije ROSa i akumulaciju oksidovanog glutationa u plazmi, dok je glutationski sistem pokazao efikasan antioksidativni kapacitet u RBCu pacova. Kada se uporede dve anemične grupe, izraženiji oksidacioni stres se javlja u plazmi pacova tretiranih PHZom. Projekat ministarstva br. 143035B

Published
2010

45. Vremenski zavisan tok eksperimentalne anemije u pacova izazvane dnevnim krvarenjem

Author

Marković, Snežana D., Milošević, Marijana P., Đorđević, Nataša Z., Ognjanović, Branka I., Štajn, A.Š., Saičić, Zorica, and Spasić, Mihajlo

Subjects
rats, hematological parameters, bleeding, anemia
Abstract

In order to investigate daily changes of hematological parameters in bleeding-induced anemia, we treated Wistar albino male rats by daily bleeding (1.5-2 mL of blood from the tail vein for eight days). Blood samples were taken before (on day zero) and on the first to eighth days of bleeding. The values of hematocrit, hemoglobin, and erythrocyte count decreased significantly after the second, sixth, and second days of bleeding, respectively. The number of leuko­cytes and platelets, as well as Heinz body levels, increased significantly after the third and second days of treatment. The percentage of reticulocytes increased significantly from the second day and attained the maximum level (32.55 ± 0.96%) on the eighth day. U cilju ispitivanja dnevnih promena hematoloških parametara u toku anemije indukovane dnevnim krvarenjem, tretirali smo Wistar albino pacove dnevnim krvarenjem (1.5-2 ml krvi iz repne vene u toku 8 dana). Uzorci krvi uzimani su pre (0 dan) i 1-8. dana krvarenja. Vrednosti za hematokrit, hemoglobin i broj eritrocita značajno opadaju nakon drugog, šestog i drugog dana krvarenja. Broj leukocita i trombocita, kao i nivo formiranja Heinz-ovih telašaca raste značajno nakon trećeg i drugog dana tretmana. Procenat retikulocita značajno raste od drugog dana i dostiže maksimalni nivo (32.55 ± 0.96 %) osmog dana tremana. Projekat ministarstva br. 143035B

Published
2009

46. Aktivnost enzima antioksidacione zaštite, koncentracija glutationa i lipidnih peroksida u jetri dve vrste riba iz Jadranskog mora

Author

Filipović, Jasmina Ž., Nikolić, Dubravka M., Ognjanović, Branka I., Marković, Snežana D., Žikić, Radoslav V., Štajn, Andraš Š., Petrović, Vojislav M., and Saičić, Zorica

Subjects
antioxidant enzymes, lipid peroxidation, glutathione, hake, red mullet
Abstract

Fish are the most important organisms used in biomonitoring of aquatic ecosystems. Parameters of oxidative stress in fish tissues represent significant biomarkers in the assessment of the status of environment. Specimens of hake (Merluccius merluccius L) and red mullet (Mullus barbatus L) were collected from the locality in front of sea-port Bar (South Adriatic Sea) at the end of May 2005. Activity of the enzymes of the antioxidative system (superoxide dismutase - SOD and catalase - CAT) and parameters of oxidative stress (lipid peroxidation - LP and reduced glutathione - GSH content) were determined in the liver of hake and red mullet. Obtained results showed that the activities of SOD and CAT were significantly lower in the liver of hake than in red mullet. Higher concentrations of LP and GSH in liver of hake were recorded, due to the changes of physical-chemical parameters of environment. The intensity of oxidative stress in liver of hake was higher in comparison to red mullet. Ribe predstavljaju najznačajnije organizme koji se koriste u biomonitoringu vodenih ekosistema. Parametri oksidacionog stresa u tkivima riba predstavljaju značajne biomarkere u proceni stanja životne sredine. Krajem maja 2005. godine uzorkovane su jedinke oslića (Merluccius merluccius L) i trlje (Mullus barbatus L) sa lokaliteta ispred luke Bar (Južni Jadran). Aktivnost enzima antioksidacionog sistema (superoksid dismutaze - SOD i katalaze - CAT) i parametri oksidacionog stresa (koncentracija lipidnih peroksida - LP i redukovanog glutationa - GSH) su određivani u jetri oslića i trlje. Dobijeni rezultati su pokazali da su aktivnosti SOD i CAT značajno manje u jetri oslića nego kod trlje. Zabeležena je veća koncentracija LP i GSH u jetri oslića, kao posledica promena fizičko-hemijskih parametara sredine i prisustva polutanata. Intenzitet oksidacionog stresa u jetri oslića je bio viši u odnosu na trlju. Projekat ministarstva br. 143035B

Published
2006

47. Energetski status u krvi i eritrocitima trudnica sa preeklampsijom

Author

Marković, Snežana D., Dragićević-Đoković, Ljiljana M., Đorđević, Nataša Z., Ognjanović, Branka I., Štajn, Andraš Š., Žikić, Radoslav V., Petrović, Vojislav M., Babić, Goran M., and Saičić, Zorica

Subjects
preeclampsia, energy status, whole blood, erythrocytes, glycolysis
Abstract

Preeclampsia represents a pregnancy specific syndrome characterized by elevated arterial blood pressure and proteinuria. The aim of this study was to evaluate whole blood and erythrocytes energy status in healthy and preeclamptic women. Our study involved 18 healthy (control) and 19 preeclamptic women. Diagnosis of preeclampsia was done according to the recommendation of International Society for the Study of Hypertension in Pregnancy. After blood collection and erythrocytes extraction, the next parameters were followed: concentrations of lactate and adenine nucleotides (ATP, ADP and AMP). Results showed increased concentrations of lactate and ATP in whole blood of preeclamptic, vs. control pregnant women. All parameters of energy status were also elevated in erythrocites of preeclamptic women (elevation of ADP level was significant). This study suggests that preeclamsia was followed by an elevation of all parameters of energy status in whole blood and in erythrocytes, as a result of increased rate of glycolysis. . Preeklampsija predstavlja za trudnoću specifičan sindrom koji se karakteriše povišenim krvnim pritiskom i proteinurijom. Cilj istraživanja je određivanje energetskog statusa pune krvi i eritrocita zdravih i trudnica sa preeklampsijom. Ispitivanjem je bilo obuhvaćeno 18 zdravih i 19 trudnica sa preeklampsijom. Dijagnoza preeklampsije je postavljena prema preporukama International Society for the Study of Hypertension in Pregnancy. Nakon uzimanja krvi i ekstrakcije eritrocita praćeni su sledeći parametri: koncentracije laktata i adenin nukleotida (ATP, ADP i AMP). Rezultati su pokazali povećanje koncentracija laktata i ATP u punoj krvi trudnica sa preeklampsijom u odnosu na kontrolne trudnice. U eritrocitima, svi parametri energetskog statusa su bili povećani u preeklampsiji (povećanje ADP nivoa je bilo značajno). Ova studija pokazuje da je preeklampsija praćena povećanjem svih parametara energetskog statusa u punoj krvi i eritrocitima, kao posledica stimulisanog intenziteta procesa glikolize. . null

Published
2006

48. Koncentracije lipidnih peroksida i glutationa u jetri i belom mišiću oslića (Merluccius merluccius L.) i arbuna (Pagellus erythrinus L.) iz Jadranskog mora

Author

Ognjanović, Branka I., Filipović, Jasmina Ž., Marković, Snežana D., Žikić, Radoslav V., Štajn, Andraš Š., Petrović, Vojislav M., and Saičić, Zorica

Subjects
Adriatic Sea, lipid peroxidation, glutathione, hake, sea bream
Abstract

Fish are the most important organisms used in biomonitoring of aquatic ecosystems. Parameters of oxidative stress in fish tissues represent significant biomarkers in the assessment of the status of environment. Specimens of hake (Merluccius merluccius L.) and sea bream (Pagellus erythrinus L.) were collected at the end of May 2005 from the locality in front of sea-port Bar (South Adriatic Sea). Parameters of oxidative stress (concentration of lipid peroxidase - LP and reduced glutathione - GSH) were determined in the liver and white muscle of hake and sea bream. Physical-chemical parameters (salinity, concentration of dissolved oxygen and temperature) were determined in water of the investigated locality. Obtained results showed that the concentration of LP was higher in liver of hake and sea bream in comparison to white muscle. However, significantly higher concentration of GSH was found in white muscle of hake and sea bream in comparison to liver. These changes of oxidative stress parameters point to tissue specificity, which is the consequence of different metabolic and antioxidative activity. Inter-specific differences were also established. Higher concentrations of LP and GSH in liver of hake and in white muscle of sea bream were recorded, due to the changes of physical-chemical parameters of environment. The intensity of oxidative stress in liver of hake and sea bream was higher in comparison to muscle. Ribe predstavljaju najznačajnije organizme koji se koriste u biomonitoringu vodenih ekosistema. Parametri oksidacionog stresa u tkivima riba predstavljaju značajne biomarkere u proceni stanja životne sredine. Krajem maja 2005. godine uzorkovane su jedinke oslića (Merluccius merluccius L.) i arbuna (Pagellus erythrinus L.) sa lokaliteta ispred luke Bar (Južni Jadran). Parametri oksidacionog stresa (koncentracija lipidnih peroksida - LP i redukovani glutationa - GSH) su određivani u tkivima jetre i belog mišića oslića i arbuna. U vodi ispitivanog lokaliteta određivani su i fizičko-hemijski parametri (salinitet, koncentracija rastvorenog kiseonika i temperatura). Dobijeni rezultati su pokazali da je koncentracija LP veća u jetri oslića i arbuna u odnosu na belo mišićno tkivo. Međutim, zabeležena je znatno veća koncentracija GSH u belom mišiću i oslića i arbuna u odnosu na jetru. Ovakve promene parametara oksidacionog stresa ukazuju na tkivnu specifičnost, kao posledica različite metaboličke i antioksidacione aktivnosti. Ustanovljene su i interspecijske razlike, zabeležena je veća koncentracija LP i GSH u jetri oslića, kao i u belom mišiću arbuna, kao posledica promena fizičko-hemijskih parametara sredine i prisustva polutanata. Intenzitet oksidacionog stresa u jetri oslića i arbuna je bio viši u odnosu na mišiće. null

Published
2006

49. Kadmijumom indukovane promene u hemato-biohemijskim parametrima, lipidnoj peroksidaciji i sadržaju glutationa u krvi pacova

Author

Ognjanović, Branka I., Marković, Snežana D., Pavlović, Slađan, Žikić, Radoslav V., Štajn, Andraš Š., and Saičić, Zorica

Subjects
cadmium, blood, hemato-biochemical parameters, lipid peroxidation, rat, glutathione
Abstract

Cadmium (Cd+2) is an ubiquitous toxic metal that may induce oxidative damage by disturbing the prooxidant-antioxidant balance in the blood. Wistar albino rats (3 months old), were injected with a single dose of CdCh (0.4 mg Cd/kg i.p. and sacrificed after 24h). The hematological parameters: red blood cells count (RBCs), hematocrite (Ht) value and hemoglobin (Hb) concentration were significantly decreased in the blood of Cd treated rats. The activities of enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in plasma, as well as concentration of blood glucose were significantly increased in animals treated with cadmium in comparison to control values. The treatment with Cd increased lipid peroxidation (LP) and reduced glutathione (GSH) contents in the blood, suggesting that the Cd induced oxidative stress. These results of our study suggested that Cd induced alterations in hemato-biochemical parameters and LP and GSH content. Kadmijum (Cd+2) je široko rasprostranjen toksičan metal koji može izazvati oksidaciona oštećenja u krvi remeteći prooksidaciono-antioksidacionu ravnotežu. Wistar albino pacovi (stari 3 meseca), tretirani su pojedinačnim dozama CdCl; (0.4 mg Cd/kg i.p) i žrtvovani posle 24 časa. Hematološki parametri, i to: broj eritrocita (RBCs), hematokritska vredno.st (Ht) i koncentracija hemoglobina (Hb) značajno su smanjeni u krvi tretiranih pacova. Aktivnost enzima alanin aminotransaminaze (ALT) i aspartat aminotransaminaze (AST) u plazmi, kao i koncentracija glukoze u krvi značajno su povećani kod životinja tretiranih kadmijumom u poređenju sa kontrolnim vrednostima. Tretman sa kadmijumom, takođe je doveo do povećanja lipidne peroksidacije (LP) i redukovanog glutationa (GSH). Sve to ukazuje da je kadmijum izazvao oksidacioni stres u krvi pacova. Dobijeni rezultati ukazuju da je Cd indukovao promené u hemato-biohemijskim parametrima, kao i u sadržaju LP i GSH u krvi tretiranih pacova. null

Published
2005

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