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The existing data for wells in the Weyburn-Midale Field were used in conjunction with publicly available data to create an updated well integrity risk assessment as an aid in establishing CO 2 containment risk and developing a guide to selection of monitoring technologies for risk management. The assessment built upon an existing assessment to rank 1424 wells on 13 categories used as a proxy for likelihood of CO 2 migration and four categories used as a proxy for severity of impact of migration. The results of the assessment were used to show that both the risk posed by individual wells and risk posed by categories may be significant with respect to CO 2 migration. The location of the top of cement, quality of the casing, and type of well categories were ranked high throughout the distribution of wells with respect to likelihood of migration. The assessment of casing integrity and setting of casing through the Midale Evaporite was a differentiating factor between higher-likelihood and lower-likelihood well scores. The aquifer protection category used in assessing the severity of impact ranked high across the distribution of wells. The proximity to a water source category was a differentiating factor between higher-severity and lower-severity rankings. The wells scores and ranking categories were used to assess the value of specific monitoring tools for managing containment risks. The outcome of the study showed that both high risk wells and high-risk categories can be identified and that a monitoring program should be adopted to address both through point and area monitoring.

We have constructed a physical map of the human genome by using a panel of 83 whole genome radiation hybrids (the Stanford G3 panel) in conjunction with 10,478 sequence-tagged sites (STSs) derived from random genomic DNA sequences, previously mapped genetic markers, and expressed sequences. Of these STSs, 5049 are framework markers that fall into 1766 high-confidence bins. An additional 945 STSs are indistinguishable in their map location from one or more of the framework markers. These 5994 mapped STSs have an average spacing of 500 kb. An additional 4484 STSs are positioned with respect to the framework markers. Comparison of the orders of markers on this map with orders derived from independent meiotic and YAC STS-content maps indicates that the error rate in defining high-confidence bins is

The human genome is thought to harbor 50,000 to 100,000 genes, of which about half have been sampled to date in the form of expressed sequence tags. An international consortium was organized to develop and map gene-based sequence tagged site markers on a set of two radiation hybrid panels and a yeast artificial chromosome library. More than 16,000 human genes have been mapped relative to a framework map that contains about 1000 polymorphic genetic markers. The gene map unifies the existing genetic and physical maps with the nucleotide and protein sequence databases in a fashion that should speed the discovery of genes underlying inherited human disease. The integrated resource is available through a site on the World Wide Web at http://www.ncbi.nlm.nih.gov/SCIENCE96/.

We have constructed a physical map of the human genome by using a panel of 90 whole-genome radiation hybrids (the TNG panel) in conjunction with 40,322 sequence-tagged sites (STSs) derived from random genomic sequences as well as expressed sequences. Of 36,678 STSs on the TNG radiation hybrid map, only 3604 (9.8%) were absent from the unassembled draft sequence of the human genome. Of 20,030 STSs ordered on the TNG map as well as the assembled human genome draft sequence and the Celera assembled human genome sequence, 36% of the STSs had a discrepant order between the working draft sequence and the Celera sequence. The TNG map order was identical to one of the two sequence orders in 60% of these discrepant cases.

This study examined the effects of partial sleep deprivation on submaximal and maximal weight-lifting tasks and on subjective states pre- and post-activity. Eight male subjects (aged 18-24 years) were restricted to a nightly ration of 3 h sleep for 3 successive nights after baseline measures on the first day. A 4 day period where normal sleep was permitted fulfilled a control condition, the normal and sleep-deprived conditions being counterbalanced and separated by 10 days. The weight-lifting tasks consisted of biceps curl, bench press, leg press, and dead lift. For each exercise a submaximal load, corresponding to a fixed value on a category ratio scale of exertion, was determined for 20 repetitions; the maximal lift for that exercise was then obtained. A profile of mood states and subjective sleepiness were determined at each test occasion, tests being conducted in the evening of each day. There was no significant effect of sleep loss on performance of maximal biceps curl (p0.05) but a significant effect was noted on maximal bench press, leg press, and dead lift (p0.001). Trend analysis indicated decreased performance in submaximal lifts for all the 4 tasks: the deterioration was significant after the second night of sleep loss (p0.01). Performing the lifts had little influence on sleepiness ratings which increased linearly with successive days of sleep loss. Mood states of confusion, vigour, and fatigue were affected significantly by the sleep deprivation regimen (p0.001), but there was no significant effect of sleep loss or anger, tension, and depression (p0.05).(ABSTRACT TRUNCATED AT 250 WORDS)