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9 results on '"Marjorie Bideau"'

1. Sources of variation of DNA methylation in rainbow trout: combined effects of temperature and genetic background

Author

Delphine Lallias, Maria Bernard, Céline Ciobotaru, Nicolas Dechamp, Laurent Labbé, Lionel Goardon, Jean-Michel Le Calvez, Marjorie Bideau, Alexandre Fricot, Audrey Prézelin, Mathieu Charles, Marco Moroldo, Xavier Cousin, Olivier Bouchez, Alain Roulet, Edwige Quillet, and Mathilde Dupont-Nivet

Subjects
temperature, epiradseq, dna methylation, dnmt3, rainbow trout, isogenic lines, Genetics, QH426-470
Abstract

Phenotypic plasticity is a key component of the ability of organisms to respond to changing environmental conditions. In this study, we aimed to study the establishment of DNA methylation marks in response to an environmental stress in rainbow trout and to assess whether these marks depend on the genetic background. The environmental stress chosen here was temperature, a known induction factor of epigenetic marks in fish. To disentangle the role of epigenetic mechanisms such as DNA methylation in generating phenotypic variations, nine rainbow trout isogenic lines with no genetic variability within a line were used. For each line, half of the eggs were incubated at standard temperature (11°C) and the other half at high temperature (16°C), from eyed-stage to hatching. In order to gain a first insight into the establishment of DNA methylation marks in response to an early temperature regime (control 11°C vs. heated 16°C), we have studied the expression of 8 dnmt3 (DNA methyltransferase) genes, potentially involved in de novo methylation, and analysed global DNA methylation in the different rainbow trout isogenic lines using LUMA (LUminometric Methylation Assay). Finally, finer investigation of genome-wide methylation patterns was performed using EpiRADseq, a reduced-representation library approach based on the ddRADseq (Double Digest Restriction Associated DNA) protocol, for six rainbow trout isogenic lines. We have demonstrated that thermal history during embryonic development alters patterns of DNA methylation, but to a greater or lesser extent depending on the genetic background.

Published
2021
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2. Analysis of genomewide patterns of DNA methylation in response to an early temperature stress in rainbow trout

Author

Delphine Lallias, Edwige Quillet, Maria Bernard, Céline Ciobotaru, Nicolas Dechamp, Jean-Michel Le Calvez, Marjorie Bideau, Lionel Goardon, Laurent Labbé, Mathilde Dupont-Nivet, Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Pisciculture Expérimentale INRA des Monts d'Arrée (PEIMA), and Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2019

3. Germ stem cells transplantation in fish: an innovative biotechnology for the faithfull regeneration of cryopreserved genetic ressources collected from selected lines

Author

Jean-Jacques Lareyre, Anne-Sophie Goupil, Ahmed Maouche, Alexandra Depince, Lionel Goardon, Marjorie Bideau, Nicolas Dechamp, Edwige Quillet, Francine Krieg, Florence Le Gac, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Pisciculture Expérimentale INRA des Monts d'Arrée (PEIMA), Institut National de la Recherche Agronomique (INRA), Génétique Animale et Biologie Intégrative (GABI), and Institut National de la Recherche Agronomique (INRA)-AgroParisTech

Subjects
fish, reproduction, poisson, [SDV]Life Sciences [q-bio], cryofixation, technological change, innovation technique, conservation des ressources génétiques, cellule souche germinale, cryoconservation
Abstract

Germ stem cells transplantation in fish: an innovative biotechnology for the faithfull regeneration of cryopreserved genetic ressources collected from selected lines. Aqua 2018

Published
2018

4. Germ stem cells transplantation in fish: an innovative biotechnology for the faithful regeneration of cryopreserved genetic resources collected from selected lines of agronomic interest

Author

Jean-Jacques Lareyre, Anne-Sophie Goupil, Ahmed Maouche, Alexandra Depince, Lionel Goardon, Marjorie Bideau, Nicolas Dechamp, Edwige Quillet, Francine Krieg, Florence Le Gac, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Pisciculture Expérimentale INRA des Monts d'Arrée (PEIMA), Institut National de la Recherche Agronomique (INRA), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, European Aquaculture Society (EAS)., and European Project: 652831,H2020,H2020-INFRAIA-2014-2015,AQUAEXCEL2020(2015)

Subjects
[SDV.GEN]Life Sciences [q-bio]/Genetics, sélection génétique, [SDV]Life Sciences [q-bio], régénération cellulaire, ovocyte, cellule souche germinale, cryoconservation, reproduction, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, poisson, aquaculture, embryon, transplantation cellulaire, [INFO]Computer Science [cs], conservation des ressources génétiques, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2018

5. Analysis of genetic variability of DNA methylation in response to an early temperature stress in rainbow trout

Author

Delphine Lallias, Edwige Quillet, Maria Bernard, Nicolas Dechamp, Jean-Michel Le Calvez, Marjorie Bideau, Lionel Goardon, Laurent Labbé, Mathilde Dupont-Nivet, Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris Saclay (COmUE), Institut National de la Recherche Agronomique (INRA), Pisciculture Expérimentale INRA des Monts d'Arrée (PEIMA), and European Project: 652831,H2020,H2020-INFRAIA-2014-2015,AQUAEXCEL2020(2015)

Subjects
DNA methylation, epigenetics, early stress, [SDV.BA]Life Sciences [q-bio]/Animal biology, temperature, rainbow trout, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

6. Etude de la variabilité génétique de la méthylation de l'ADN en réponse à un stress de température précoce chez la truite arc-en-ciel

Author

Delphine Lallias, Edwige Quillet, Nicolas Dechamp, Maria Bernard, Jean-Michel Le Calvez, Marjorie Bideau, Isabelle Leguen, Patrick Prunet, Lionel Goardon, Laurent Labbé, Mathilde Dupont-Nivet, Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Pisciculture Expérimentale INRA des Monts d'Arrée (PEIMA), Institut National de la Recherche Agronomique (INRA), Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Crédit incitatif Département GA, and European Project: 652831,H2020,H2020-INFRAIA-2014-2015,AQUAEXCEL2020(2015)

Subjects
Animal biology, [SDV.BA]Life Sciences [q-bio]/Animal biology, Biologie animale, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2018

7. Temporal changes in the growth of two Mediterranean cold-water coral species, in situ and in aquaria

Author

Nadine Le Bris, Simon Pareige, Pascal Romans, Elwyn de la Vega, Franck Lartaud, Marc de Rafélis, Erwan Peru, Lionel Feuillassier, Marjorie Bideau, Karine Nedoncelle, Laboratoire d'Ecogéochimie des environnements benthiques (LECOB), Observatoire océanologique de Banyuls (OOB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Biominéralisations et environnements sédimentaires (BES), Institut des Sciences de la Terre de Paris (iSTeP), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), and Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Canyon, Mediterranean climate, geography, geography.geographical_feature_category, biology, Coral, media_common.quotation_subject, [SDU.STU]Sciences of the Universe [physics]/Earth Sciences, Oceanography, biology.organism_classification, Lophelia, Mediterranean sea, Ecosystem, 14. Life underwater, Reproduction, ComputingMilieux_MISCELLANEOUS, media_common, Madrepora oculata
Abstract

In situ growth patterns of two-species of reef-building cold-water corals were investigated for the first time at different temporal scales, based on the redeployment of coral nubbins in their natural environment. Lophelia pertusa and Madrepora oculata were collected in November 2010 and May 2011 from the Lacaze-Duthiers canyon in the northwestern Mediterranean Sea (520 m depth). Three in situ growth experiments were performed from November 2010 to May 2011, May to September 2011 and November 2010 to September 2011. For comparison, aquaria experiments over comparable lengths of time were conducted with coral colonies collected in November 2010. In the canyon, new polyps from M. oculata and L. pertusa displayed similar growth rates in summer (5.8±3.8 and 7.3±1.7 mm yr−1 respectively), but M. oculata growed significantly slower during winter/spring than L. pertusa (4.1±1.8 and 8.4±2.7 mm yr−1 respectively). Budding rates however (the rate of new polyp addition per mother polyp per year) were similar between both species in winter/spring (45±40% for M. oculata and 48±72% for L. pertusa), but were significantly lower in summer for M. oculata (14±19%) compared to L. pertusa (58±94%). This seasonal difference in the growth between L. pertusa and M. oculata might reflect differences in species-specific physiology (such as reproduction) or feeding strategy, or a higher sensitivity of M. oculata to the variability of food supply in the Lacaze-Duthiers canyon resulting from periodic cascading events. The comparison of in situ and aquaria growth experiments showed no significant differences for budding and new polyp growth rates, which supports the validity of aquaria experiments for these kinds of investigations. However the budding rates observed were consistently lower in coral maintained in aquaria than those in in situ conditions, a finding which is to be considered when extrapolating laboratory based investigation results to the naturally occurring coral ecosystem.

Published
2014
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8. A new approach for assessing cold-water coral growth in situ using fluorescent calcein staining

Author

Nadine Le Bris, Pascal Romans, Frédéric Alcala, Marc de Rafélis, Franck Lartaud, Erwan Peru, Marjorie Bideau, Lionel Feuillassier, Simon Pareige, Laboratoire d'Ecogéochimie des environnements benthiques (LECOB), Observatoire océanologique de Banyuls (OOB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Biominéralisations et environnements sédimentaires (BES), Institut des Sciences de la Terre de Paris (iSTeP), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), and Compagnie Maritime d'Expertise (COMEX)

Subjects
0106 biological sciences, In situ, geography, geography.geographical_feature_category, 010504 meteorology & atmospheric sciences, biology, Ecology, 010604 marine biology & hydrobiology, Scleractinia, [SDU.STU]Sciences of the Universe [physics]/Earth Sciences, Coral reef, Aquatic Science, biology.organism_classification, 01 natural sciences, Calcein, chemistry.chemical_compound, Animal science, Mediterranean sea, Lophelia, chemistry, 14. Life underwater, Reef, ComputingMilieux_MISCELLANEOUS, 0105 earth and related environmental sciences, Madrepora oculata
Abstract

Research on the biology and ecology of cold-water corals (CWCs) is still in its infancy. The growth patterns of CWCs in their natural environments are poorly known. Growth rate investigations on these deep-sea reef builder species are needed to predict recovery times following damage to their ecosystems. This study investigates a new approach for analysing CWC growth rate, suitable for in situ application. Lophelia pertusa and Madrepora oculata (Scleractinian) were collected from the Lacaze-Duthiers canyon in the northwestern Mediterranean Sea (520 m depth), marked and then either redeployed in situ for 6 months, or maintained in aquaria for growth rate comparison at a constant temperature of 13 °C, corresponding to their habitat conditions. Two different types of staining (calcein and manganese) and three different exposure times (30, 60 and 240 min) were tested. The results show that calcein offers rapid incorporation and easy detection, making it particularly suitable for skeletal growth rate investigations compared with other chemical staining. In situ linear polyp growth rates of 7.5 ± 1.2 mm y-1 and 3.5 ± 2.1 mm y-1 were measured in new polyps of L. pertusa and M. oculata , respectively. Those values were significantly higher in young polyps than in older ones, where they decreased to 1.3 ± 1.5 mm y-1 and 1.2 ± 1.2 mm y-1 . Beyond the study of coral reef growth processes, this approach offers a methodological basis for habitat quality assessment which could be used in the management of deep-sea marine protected areas (MPA).

Published
2013
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9. Implementation of a germ stem cell transplantation procedure for the regeneration of isogenic trout lines

Author

Anne-Sophie Goupil, Ahmed Maouche, Alexandra Depince, Lionel Goardon, Marjorie Bideau, Nicolas Dechamp, Edwige Quillet, Francine Krieg, Jean-Jacques Lareyre, Florence Le Gac, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Pisciculture Expérimentale INRA des Monts d'Arrée (PEIMA), Institut National de la Recherche Agronomique (INRA), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, This study was supported by the UE AquaExcel 2020 project., and University of South Bohemia in České Budějovice. CZE.

Subjects
fish, spermatogonial stem cells, endocrine system, trout, health care facilities, manpower, and services, [SDV]Life Sciences [q-bio], education, triploid recipient, truite, cellule souche germinale, spermatogonia, triploïde, reproduction, isogenic line, poisson, spermatogonie, aquaculture, transplantation cellulaire, conservation des ressources génétiques, health care economics and organizations, transplantation, lignée isogénique
Abstract

International audience; Implementation of a germ stem cell transplantation procedure for the regeneration of isogenic trout lines. 6. International Workshop on the Biology of Fish Gametes

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