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Recent global trade disruptions, due to blockage of the Suez Canal and cascading effects of COVID-19, have altered the movement patterns of commercial ships and may increase worldwide invasions of marine non-indigenous species. Organisms settle on the hulls and underwater surfaces of vessels and can accumulate rapidly, especially when vessels remain stationary during lay-ups and delays. Once present, organisms can persist on vessels for long-periods (months to years), with the potential to release propagules and seed invasions as ships visit ports across the global transportation network. Shipborne propagules also may be released in increasing numbers during extended vessel residence times at port or anchor. Thus, the large scale of shipping disruptions, impacting thousands of vessels and geographic locations and still on-going for over two years, may elevate invasion rates in coastal ecosystems in the absence of policy and management efforts to prevent this outcome. Concerted international and national biosecurity actions, mobilizing existing frameworks and tools with due diligence, are urgently needed to address a critical gap and abate the associated invasion risks.

Spread of nonindigenous organisms by shipping is one of the largest threats to coastal ecosystems. Limited monitoring and understanding of this phenomenon currently hinder development of effective prevention policies. Surveying ports in North America, South America, Europe, Southeast Asia, and Australia we explored environmental DNA community profiles evident of ship-born species spread. We found that community similarities between ports increased with the number of ship voyages, particularly if the ports had similar environments, and when indirect stepping-stone connections were considered. We also found 57 known non-indigenous taxa, some in hitherto unreported locations. We demonstrate the usefulness of eDNA-based tools for global biodiversity surveys, and highlight that shipping homogenizes biodiversity in predictable that could inform policy and management.

Biofouling is a long-standing challenge for ships because it can interfere with operations and increases vessel drag, fuel consumption and exhaust emissions. More recently, ship biofouling has also been recognized as a leading vector for global transfers and introductions of marine non-indigenous species. Ship in-water cleaning and capture (IWCC) systems, to remove and collect macrofouling organisms and associated antifouling coating compounds, are now becoming available as a possible solution to both problems. However, independent and rigorous evaluations of IWCC efficacy and environmental safety are needed to facilitate technology maturation, support vessel operator biofouling management decisions, aid IWCC approvals and permitting, and inform future biosecurity regulations. We developed a formal protocol for evaluating an IWCC system, on two ships with varying biofouling levels and under different environmental conditions, to quantify biofouling removal and capture efficacy as well as impacts on water quality. The IWCC system reduced hull biofouling by 82–94%. Concentrations of dissolved and particulate Cu and Zn in effluent from the IWCC onshore processing varied by orders of magnitude between trials, in one case greatly exceeding water quality standards. Our results demonstrate that rigorous, quantitative assessments of IWCC system performance are possible, even under challenging conditions. This initial evaluation also identifies the major factors that impact performance of in-water cleaning, and key needs for future research to consider in advancing standardized testing and independent evaluations needed for all in-water cleaning systems.

National and international regulations require that ships' ballast water is treated to minimize the risk of introducing potentially invasive species. A common approach employed by commercial ballast water management systems is chlorination. This study presents the algal toxicity findings for three chlorination-based BWMS and their implications to environmental safety of port waters receiving treated ballast water from ships. Discharged treated ballast water from all three BWMS was toxic to algae with IC25s (25% growth inhibition) ranging from 9.9% to 17.9%, despite having total residual oxidant concentrations below 0.02 mg/l, based on Whole Effluent Toxicity assays. When held at 4 °C, some of the ballast water samples continued to exhibit toxic effects with no observed effect concentrations as low as 18% after a 134 day holding time. Thirteen individual disinfection by-products were measured above the detected limit at the time of discharge. No correlation between DBPs and algal toxicity was observed.

International and national guidelines and regulations to limit the inadvertent transfer of non-native species on the submerged surfaces of vessels and mobile infrastructure are progressing. However, methods to assess compliance must be developed to assist both regulators and industry. While there is a history of biofouling inspections in maritime industries, including commercial shipping and infrastructure, such surveys are tailored for vessel safety and performance rather than being driven by biosecurity purposes. Thus, these inspections are likely inadequate for confirming compliance with biosecurity regulations. To determine regulatory compliance, agencies will likely rely on a combination of risk profiling, assessment of documentation of biofouling management, archival data and images, and real-time in-water surveys made by divers or remotely operated vehicles (ROVs) specific to biosecurity regulations. Divers may exceed ROVs at finding organisms in recesses and other topographically complex areas, and when regulations require confirmation of species identity or viability. In contrast, ROVs may be well suited for regulations that establish upper thresholds on biofouling levels with little concern for organism identity or condition. Several factors will inform how a survey is conducted, including cost, the type of data required by regulations, environmental conditions, safety, and logistics. Survey designs and requirements should be transparent to manage industry’s expectations of border procedures, to increase the efficiency with which industry and agencies manage biofouling and potentially align the evaluation of best practices in hull and niche area maintenance across jurisdictions.

Niche areas of ships, such as lateral thruster tunnels, sea chests, and propellers, are often hot spots for the accumulation of biofouling organisms, a potential source of aquatic invasive species. Yet, the relative importance of different niche areas is poorly resolved, in terms of both total surface area and the associated biota (i.e., the species of organisms and their abundances). To address this information gap, a method was developed to estimate the extent of various niche areas in the global fleet of 120,252 commercial ships active between 1999 and 2013. The total niche area for these vessels was estimated to be 32,996 × 103 m2, representing approximately 10% of the total wetted surface area (WSA) available for colonization by biota. Considering the portion of niche areas relative to the total WSA, it was highest for passenger vessels (27%), followed by tugs (25%), and fishing vessels (21%), with niche areas representing a small portion of the WSA for bulk carriers and tankers (7–8%). Examining the different types of niche areas, thruster tunnels had the greatest total extent (10,189 × 103 m2), representing a disproportionately large contribution (>50%) of the total niche area for passenger vessels and tugs compared to other vessel types. This result, combined with the use and cleaning of thrusters, may render them “super-hot spots” of biofouling. The uneven distribution and extent of niche areas across vessels has implications for transfers of organisms and management strategies to reduce invasions associated with the surfaces of ships.

Scientists who observe and distribute oceanographic data require a process to ensure high-quality data. This process includes quality assurance, quality control, quality assessment, standards, and best practices. In this paper, quality assurance is widely regarded as actions taken prior to instrument deployment to improve the probability of generating good data, while quality control is the effort made to examine the resultant data. Herein we focus on quality assurance and strive to guide the oceanographic community by identifying existing quality assurance best practices preferred by the five entities represented by the authors – specifically, the Alliance for Coastal Technology, the AtlantOS project, the Integrated Marine Observing System, the Joint Technical Commission for Oceanographic and Marine Meteorology, and the U.S. IOOS Quality Assurance/Quality Control of Real-Time Oceanographic Data project. The focus has been placed on QA in response to suggestions from the AtlantOS and QARTOD communities. We define the challenges associated with quality assurance, which include a clear understanding of various terms, the overlap in meaning of those terms, establishment of standards, and varying program requirements. Brief, “real-world” case-studies are presented to demonstrate the challenges. Following this is a description of best practices gathered by the authors from hundreds of scientists over the many years or decades the aforementioned entities have been in place. These practices address instrument selection, preparation, deployment, maintenance, and data acquisition. Varying resources and capabilities are considered, and corresponding levels of quality assurance efforts are discussed. We include a comprehensive description of measurement uncertainty with a detailed example of such a calculation. Rigorous estimates of measurement uncertainty are surprisingly complex, necessarily specific, and not provided as often as needed. But they are critical to data users who may have applications not envisioned by the data provider, to ensure appropriate use of the data. The guidance is necessarily generic because of the broad expanse of oceanographic observations. Further, it is platform-agnostic and applies to most deployment scenarios. We identify the recently created Ocean Best Practice System as one means of developing, sharing, documenting, and curating more specific QA processes. Ultimately, our goal here is to foster their development and harmonization.

Recurrent blooms of harmful algae and cyanobacteria (HABs) plague many coastal and inland waters throughout the United States and have significant socioeconomic impacts to the adjacent communities. Notable HAB events in recent years continue to underscore the many remaining gaps in knowledge and increased needs for technological advances leading to early detection. This review summarizes the main research and management priorities that can be addressed through ocean observation-based approaches and technological solutions for harmful algal blooms, provides an update to the state of the technology to detect HAB events based on recent activities of the Alliance for Coastal Technologies (ACT), offers considerations for ensuring data quality, and highlights both ongoing challenges and opportunities for solutions in integrating HAB-focused technologies in research and management. Specifically, technological advances are discussed for remote sensing (both multispectral satellite and hyperspectral); deployable in situ detection of HAB species on fixed or mobile platforms (based on bulk or taxa-specific biomass, images, or molecular approaches); and field-based and/or rapid quantitative detection of HAB toxins (via molecular and analytical chemistry methods). Suggestions for addressing challenges to continued development and adoption of new technologies are summarized, based on a consensus-building workshop hosted by ACT, including dealing with the uncertainties in investment for HAB research, monitoring, and management. Challenges associated with choosing appropriate technologies for a given ecosystem and/or management concern are also addressed, and examples of programs that are leveraging and combining complementary approaches are highlighted.

The oceans play a key role in global issues such as climate change, food security, and human health. Given their vast dimensions and internal complexity, efficient monitoring and predicting of the planet’s ocean must be a collaborative effort of both regional and global scale. A first and foremost requirement for such collaborative ocean observing is the need to follow well-defined and reproducible methods across activities: from strategies for structuring observing systems, sensor deployment and usage, and the generation of data and information products, to ethical and governance aspects when executing ocean observing. To meet the urgent, planet-wide challenges we face, methods across all aspects of ocean observing should be broadly adopted by the ocean community and, where appropriate, should evolve into “Ocean Best Practices.” While many groups have created best practices, they are scattered across the Web or buried in local repositories and many have yet to be digitized. To reduce this fragmentation, we introduce a new open access, permanent, digital repository of best practices documentation (oceanbestpractices.org) that is part of the Ocean Best Practices System (OBPS). The new OBPS provides an opportunity space for the centralized and coordinated improvement of ocean observing methods. The OBPS repository employs user-friendly software to significantly improve discovery and access to methods. The software includes advanced semantic technologies for search capabilities to enhance repository operations. In addition to the repository, the OBPS also includes a peer reviewed journal research topic, a forum for community discussion and a training activity for use of best practices. Together, these components serve to realize a core objective of the OBPS, which is to enable the ocean community to create superior methods for every activity in ocean observing from research to operations to applications that are agreed upon and broadly adopted across communities. Using selected ocean observing examples, we show how the OBPS supports this objective. This paper lays out a future vision of ocean best practices and how OBPS will contribute to improving ocean observing in the decade to come.

The International Maritime Organization and U.S. Coast Guard have implemented regulations to reduce introductions of non-indigenous species via ballast water (BW). For phytoplankton, regulations limit discharges to10 live/viable cells mL

Ballast water is used to safely stabilize and operate shipping vessels worldwide, in a multitude of aquatic settings, including inland, coastal and open oceans. However, ballast water may pose ecological, public health, and/or economic problems as it may serve as vehicles of transmission of microorganisms. Current ballast water regulations include limits of Escherichia coli, Enterococcus spp. and toxigenic Vibrio cholerae. Several United States Environmental Protection Agency approved standard operating protocols (SOPs) exist for detection of E. coli and Enterococci, yet none exists for V. cholerae. Current V. cholerae detection methods include colony blot hybridization, direct fluorescent antibody test (DFA), and/or polymerase chain reaction (PCR), which can be time consuming and difficult to perform. This study utilizes Cholera SMART II to determine its potential use in detection of V. cholerae. Validation of this method would help provide quick and accurate analysis for V. cholerae in ballast discharge waters in the field.

Ships’ hulls can transport aquatic nuisance species, but there is little quantitative information about the magnitude of vessel biofouling on a global or regional scale. There does not exist a robust method to estimate the wetted surface area (WSA) of a particular fleet of ships, especially across the diversity of possible vessel types. An estimate of the total WSA of ship arrivals into a port or region is essential to determine the potential scope of biofouling and to inform management strategies to reduce the future invasions. Multiple statistical models were developed so commonly available ships’ parameters could be used to estimate the WSA for a given set of fleet data. Using individual ship characteristics and publicly available data from ~120,000 active commercial ships in the world fleet, the method results in a total global minimum WSA estimate of approximately 325 × 106 m2. The size of the global fleet employed here is greater than the commonly cited vessel number of approximately 80,000–90,000, as we include ships

The ability of dissolved chemical cues to induce larval settlement from the water column has long been debated. Through computer-assisted video motion analysis, we quantified the movements of individual oyster (Crassostrea virginica) larvae in a small racetrack flume at free-stream flow speeds of 2.8, 6.2, and 10.4 cm/s. In response to waterborne chemical cues, but not to seawater (control), oyster larvae moved downward in the water column and swam in slow curved paths before attaching to the flume bottom. Effective stimuli were adult-oyster-conditioned seawater (OCW) and a synthetic peptide analog (glycyl-glycyl-L-arginine) for the natural cue. The chemically mediated behavioral responses of oyster larvae in flow were essentially identical to those responses previously reported in still water. Our experimental results therefore demonstrate the capacity of waterborne cues to evoke settlement behavior in oyster pediveligers under varying hydrodynamic conditions.

Erythrocytes have long served as model cells, useful for analyzing cytoskeletal structure and function. In non-mammalian vertebrates, erythrocytes are typically highly flattened, nucleated ellipsoids in which a marginal band (MB) of microtubules interacts with the membrane skeleton (MS) to generate and maintain cell shape. Though relatively rare, erythrocytes also occur in repre- sentatives of many invertebrate phyla, including the arcid and vesicomyid molluscs, but the structure and function of these cells are not well understood. Previous work has shown arcid erythrocytes to be highly flattened ellipsoids containing the MB-MS cytoskeletal system, similar to vertebrates but with an additional interesting feature: a functional centriole-containing centrosome associated with each MB. In the present study we have examined, for the first time, erythrocyte morphology and cytoskeletal structure in a vesicomyid. Using Calyptogena kilmeri, the dominant invertebrate at many Pacific cold seeps, we have found that the erythrocytes are only slightly flattened and do not contain MBs. Rather, their cytoskeletons display a peripheral centriole-containing centrosome with radiating fibers, a distinctive type of organization not observed pre- viously in mature erythrocytes from any species.

Given the societal importance of reliable and accurate ocean observations, the wave monitoring community (including academic researchers, agency scientists, resource managers, and representatives from wave instrument manufacturers) came together to develop a set of protocols for the test and evaluation of wave measurement systems in support of the 2009 National Operational Wave Observation Plan. These protocols are focused on a wide range of wave measurement instruments and their respective performance in successfully recovering the “First-5” Fourier components of the incident wave field. Performance is determined by comparing each system’s output with a verifiable reference method over a predetermined range of wave frequencies. It is recommended that permanent wave test facilities are created on the West Coast (Monterey Bay, CA—deep water) and the East Coast (Duck, NC—shallow water) for continued evaluations of existing and new technologies. It was recognized that no absolute standard exists for the determination of the “First-5” across all spatial domains. Therefore, it was agreed that the Directional Waverider DWR-MkIII system was the best available reference/standard for the deep and intermediate water wave evaluations as verified by the laser array (LASAR) at the ConocoPhillips Ekofisk offshore platform complex in the North Sea. The long linear array at the U.S. Army Corps of Engineers’ Field Research Facility could be used as the standard for shallow water wave evaluations. Finally, given the significance of wave measurements, an appropriate level of quality assurance and quality control procedures must be included as part of any test and evaluation effort. The details of the proposed protocols for the verification of wave measurement systems are described.

The effects of low-pH, high-pCO2 conditions on deep-sea organisms were examined during four deep-sea CO2 release experiments simulating deep-ocean C sequestration by the direct injection of CO2 into the deep sea. We examined the survival of common deep-sea, benthic organisms (microbes; macrofauna, dominated by Polychaeta, Nematoda, Crustacea, Mollusca; megafauna, Echinodermata, Mollusca, Pisces) exposed to low-pH waters emanating as a dissolution plume from pools of liquid carbon dioxide released on the seabed during four abyssal CO2-release experiments. Microbial abundance in deep-sea sediments was unchanged in one experiment, but increased under environmental hypercapnia during another, where the microbial assemblage may have benefited indirectly from the negative impact of low-pH conditions on other taxa. Lower abyssal metazoans exhibited low survival rates near CO2 pools. No urchins or holothurians survived during 30–42 days of exposure to episodic, but severe environmental hypercapnia during one experiment (E1; pH reduced by as much as ca. 1.4 units). These large pH reductions also caused 75% mortality for the deep-sea amphipod, Haploops lodo, near CO2 pools. Survival under smaller pH reductions (ΔpHo0.4 units) in other experiments (E2, E3, E5) was higher for all taxa, including echinoderms. Gastropods, cephalopods, and fish were more tolerant than most other taxa. The gastropod Retimohnia sp. and octopus Benthoctopus sp. survived exposure to pH reductions that episodically reached −0.3 pH units. Ninety percent of abyssal zoarcids (Pachycara bulbiceps) survived exposure to pH changes reaching ca. −0.3 pH units during 30–42 day-long experiments.

Vertical migrations of living organisms and settling of particle-attached organisms lead to uneven distributions of biota at different depths in the water column. In ballast tanks, heterogeneity could lead to different population estimates depending on the portion of the discharge sampled. For example, concentrations of organisms exceeding a discharge standard may not be detected if sampling occurs during periods of the discharge when concentrations are low. To determine the degree of stratification, water from ballast tanks was sampled at two experimental facilities as the tanks were drained after water was held for 1 or 5 days. Living organisms ≥50 μm were counted in discrete segments of the drain (e.g., the first 20 min of the drain operation, the second 20 min interval, etc.), thus representing different strata in the tank. In 1 and 5 day trials at both facilities, concentrations of organisms varied among drain segments, and the patterns of stratification varied among replicate trials. From numerical simulations, the optimal sampling strategy for stratified tanks is to collect multiple time-integrated samples spaced relatively evenly throughout the discharge event.

The development of phosphate sensors suitable for long-term in situ deployments in natural waters, is essential to improve our understanding of the distribution, fluxes, and biogeochemical role of this key nutrient in a changing ocean. Here, we describe the optimization of the molybdenum blue method for in situ work using a lab-on-chip (LOC) analyzer and evaluate its performance in the laboratory and at two contrasting field sites. The in situ performance of the LOC sensor is evaluated using hourly time-series data from a 56-day trial in Southampton Water (UK), as well as a month-long deployment in the subtropical oligotrophic waters of Kaneohe Bay (Hawaii, USA). In Kaneohe Bay, where phosphate concentrations were characteristic of the dry season (0.13 ± 0.03 μM, n = 704), the in situ sensor accuracy was 16 ± 12% and a potential diurnal cycle in phosphate concentrations was observed. In Southampton Water, the sensor data (1.02 ± 0.40 μM, n = 1,267) were accurate to ±0.10 μM relative to discrete reference samples. Hourly in situ monitoring revealed striking tidal and storm derived fluctuations in phosphate concentrations in Southampton Water that would not have been captured via discrete sampling. We show the impact of storms on phosphate concentrations in Southampton Water is modulated by the spring-neap tidal cycle and that the 10-fold decline in phosphate concentrations observed during the later stages of the deployment was consistent with the timing of a spring phytoplankton bloom in the English Channel. Under controlled laboratory conditions in a 250 L tank, the sensor demonstrated an accuracy and precision better than 10% irrespective of the salinity (0–30), turbidity (0–100 NTU), colored dissolved organic matter (CDOM) concentration (0–10 mg/L), and temperature (5–20°C) of the water (0.3–13 μM phosphate) being analyzed. This work demonstrates that the LOC technology is mature enough to quantify the influence of stochastic events on nutrient budgets and to elucidate the role of phosphate in regulating phytoplankton productivity and community composition in estuarine and coastal regimes.

Biofouling exerts a frictional and cost penalty on ships and is a direct cause of invasion by marine species. These negative consequences provide a unifying purpose for the maritime industry and biosecurity managers to prevent biofouling accumulation and transfer, but important gaps exist between these sectors. This mini-review examines the approach to assessments of ship biofouling among sectors (industry, biosecurity and marine science) and the implications for existing and emerging management of biofouling. The primary distinctions between industry and biosecurity in assessment of vessels biofouling revolve around the resolution of biological information collected and the specific wetted surface areas of primary concern to each sector. The morphological characteristics of biofouling and their effects on propulsion dynamics are of primary concern to industry, with an almost exclusive focus on the vertical sides and flat bottom of hulls and an emphasis on antifouling and operational performance. In contrast, the identity, biogeography, and ecology of translocated organisms is of highest concern to invasion researchers and biosecurity managers and policymakers, especially as it relates to species with known histories of invasion elsewhere. Current management practices often provide adequate, although not complete, provision for hull surfaces, but niche areas are well known to enhance biosecurity risk. As regulations to prevent invasions emerge in this arena, there is a growing opportunity for industry, biosecurity and academic stakeholders to collaborate and harmonize efforts to assess and manage biofouling of ships that should lead to more comprehensive biofouling solutions that promote industry goals while reducing biosecurity risk and greenhouse gas emissions.

To reduce ballast water-borne aquatic invasions worldwide, the International Maritime Organization and United States Coast Guard have each proposed discharge standards specifying maximum concentrations of living biota that may be released in ships’ ballast water (BW), but these regulations still lack guidance for standardized type approval and compliance testing of treatment systems. Verifying whether BW meets a discharge standard poses significant challenges. Properly treated BW will contain extremely sparse numbers of live organisms, and robust estimates of rare events require extensive sampling efforts. A balance of analytical rigor and practicality is essential to determine the volume of BW that can be reasonably sampled and processed, yet yield accurate live counts. We applied statistical modeling to a range of sample volumes, plankton concentrations, and regulatory scenarios (i.e., levels of type I and type II errors), and calculated the statistical power of each combination to detect noncompliant discharge concentrations. The model expressly addresses the roles of sampling error, BW volume, and burden of proof on the detection of noncompliant discharges in order to establish a rigorous lower limit of sampling volume. The potential effects of recovery errors (i.e., incomplete recovery and detection of live biota) in relation to sample volume are also discussed.

The Alliance for Coastal Technologies (ACT) has been established to support innovation and to provide the information required to select the most appropriate tools for studying and monitoring coastal and ocean environments. ACT is a consortium of nationally prominent ocean science and technology institutions and experts who provide credible performance data of these technologies through third-party, objective testing. ACT technology verifications include laboratory and field tests over short- and long-term deployments of commercial technologies in diverse environments to provide unequivocal, unbiased confirmation that technologies meet key performance requirements. ACT demonstrations of new technologies validate the technology concept and help eliminate performance problems before operational introduction. ACT’s most recent demonstration of pCO2 sensors is an example of how ACT advances the evolution of ocean observing technologies, in this case to address the critical issue of ocean acidification, and promotes more informed decision making on technology capabilities and choices.

U.S. and international rules have been proposed to reduce the risks associated with invasive aquatic organisms by requiring that ships’ ballast water be treated to kill or remove living organisms and achieve certain standards before being discharged. Enforcing these rules requires verifying when a discharge violates these standards. A preliminary comparison of verification systems indicates that mandatory reporting and inspecting treatment equipment do not provide an acceptable level of confidence and that sampling and analyzing enough ballast water to achieve acceptable confidence is prohibitively costly. The most cost-effective alternative that achieves an acceptable level of confidence involves indirect measures of ballast water using sensors that indicate whether discharge standards are met.

Particulate matter concentration (PM, often referred to as total suspended solids [TSS]) is an important parameter in the evaluation of water quality. Several optical measurements used to provide an estimate of water turbidity have also been used to estimate PM, among them light transmission, backscattering, and side-scattering. Here we analyze such measurements performed by the Alliance for Coastal Technologies (ACT) at various coastal locations to establish whether a given optical method performs better than others for the estimation of PM. All the technologies were found to perform well, predicting PM within less than 55% relative difference for 95% of samples (n = 85, four locations). Backscattering performed best as a predictor of PM, predicting PM with less than 37% relative difference for 95% of samples. The correlation coefficient (R) was between 0.96 and 0.98 for all methods with PM data ranging between 1.2 to 82.4 g m –3 . In addition, co-located measurements of backscattering and attenuation improves PM prediction and provides compositional information about the suspended particles; when their ratio is high, the bulk particulate matter is dominated by inorganic material while when low, dominated by organic material.

The Suminoe oyster (Crassostrea ariakensis) is being considered for introduction into the Chesapeake Bay. However, our current understanding of the biology and ecology of C. ariakensis is insufficient to predict whether an introduction will be successful, provide desired benefits, or have adverse impacts. Behavior of native Eastern oyster (C. virginica) pediveligers has been studied for many years and it is well established that they use a variety of habitat characteristics when selecting a site for colonization. Perhaps the most important of these are chemical cues emitted by adult conspecifics, which can lead to gregarious larval settlement and dense, persistent reef communities. Conversely, almost nothing is known about the mechanisms that regulate larval settlement and metamorphosis for C. ariakensis or how pediveligers might respond to conditions found in Chesapeake Bay. In a comparative study with C. virginica, we examined how environmental factors such as substrate type, natural biofilms, sediment and waterborne chemical cues influence larval settlement for two C. ariakensis strains (''south China'' and ''west coast''). Our results demonstrate many similarities but also potentially important differences. Both species and strains of larvae greatly prefer natural substrates (e.g., shell) covered with biofilms for colonization but the west coast strain of C. ariakensis exhibited greater attachment onto manmade substrates (e.g., fiberglass) than C. virginica. Waterborne chemical cues emitted by adult oysters were also found to enhance substrate attachment for all larval forms but cues do not appear to be species specific. These results provide critical insight to the ability of C. ariakensis larvae to identify and colonize suitable substrates in the Chesapeake Bay, which will have a large impact on recruitment success and their ability to establish self-sustaining populations.

This paper draws from recent workshops held by the Alliance for Coastal Technologies to summarize the present status of sensors and sensor platforms for making physical oceanographic observations. Technologies reviewed include those for current measurements, HF Radar, wave sensors, drifting buoys, profiling floats, surface meteorological observations, and automated vessel-based systems, as well as related issues of data telemetry and biofouling.

Marine benthic invertebrates living in dense, intraspecific aggregations are important community members because they provide structural habitat for other species. Here, we determined the mechanisms that facilitate gregarious larval settlement and promote group living. Using suspension-feeding oysters (Crassostrea gigas) residing in large assemblages (“reefs”), experiments were conducted under laboratory conditions that simulated critical aspects of natural estuarine habitats. Oyster larvae were attracted to the scent of their conspecific elders. In still-water trials, they moved downward and settled after contacting a waterborne, adult chemical cue. Yet, mortality of larvae placed in the adult pallial cavity was very high (mean of 91.3%). This seeming paradox of larval attraction to adult cannibals was resolved via laboratory flume (2 cm/s and 6 cm/s flows) experiments. Suspension-feeding activity did not significantly affect flow speeds or directions. Moreover, weak (mean of 1.65 mm/s) adult ciliary currents effectively entrained phytoplankton but rarely captured larvae. In fact, only a small percentage (≤4.6%) of settlers was cannibalized in flume trials, even when they passed within 1 mm of the inhalant opening, or “gape” (a narrow slit between two valves). Larvae cued by conspecifics potentially attach to any portion of the shell surface, but there is a low probability that they will land in or near the inhalant opening. On juvenile and adult oysters, for example, the mean ratio of gape to shell surface area was only 0.025. Furthermore, in surveys of juvenile/adult oysters at nine field sites (Hood Canal and eastern Olympic Peninsula, Washington, USA), the gape was ≤5.2% of the total plane surface area of the reef. Thus, an oyster larva settling onto a reef of suspension-feeding adults is unlikely to be cannibalized. Given this low mortality risk at settlement, future fitness payoffs (e.g., improved fertilization success) may drive the evolution of a gregarious settlement cue that promotes group living.

As regulations governing the discharge of living organisms in ships' ballast water enter into force, tools to rapidly and easily measure compliance with the discharge standards will be essential. To assess, validate, and select compliance tools, a framework-consisting of three parts-is presented: proof-of-concept, validation and verification, and final selection stages. Next, a case study describing the proof-of-concept stage is discussed. Specifically, variable fluorescence was evaluated as an approach for determining compliance with the discharge standard for living organisms ⩾10 μm and

Before the feasibility of ocean sequestration of anthropogenic carbon dioxide can be evaluated completely, there is a clear need to better understand the potential biological impacts of CO2-enriched (low pH and high pCO2) seawater in regions of proposed disposal. We describe here the first empirical study directly examining animal responses to dissolving CO2 hydrates on the deep-sea floor. Using a remotely operated vehicle (ROV) to conduct experiments within Monterey Canyon, CA, we found that several species (both invertebrate and vertebrate) did not avoid rapidly dissolving flocculent hydrates when attracted by the scent of food. Furthermore, while there were no apparent short-term effects of decreased pH, mobile animals appeared to suffer from respiratory distress due to increased pCO2 when in close proximity to hydrates. Losses of higher organisms as a result of CO2 disposal in the deep-sea may therefore be more extensive than previously predicted from toxicological models. However, the extent of changes to surrounding seawater chemistry, and thus biological impact, is largely dependent on CO2 release method or the type of hydrate formed.

Planktonic cnidarians are principal predators in the vast habitats between the ocean's surface and the deep-sea floor. Almost nothing is known, however, about the chemical ecology of these fragile midwater animals because of difficulties associated with collecting healthy specimens and con-ducting experiments in the field. With the use of a remotely operated vehicle, we found that the hydromedusa Mitrocoma cellularia is not a passive “drift-net” predator. This relatively simple gelatinous organism reacted to both the taste and smell of prey in the laboratory and in situ. Our results comprise the first definitive demonstration that a species of pelagic cnidarian responds behaviorally to chemical signals, and they lend new insight into the role of chemoreception in structuring mid-water communities.

Many deep-sea animals derive part of their nutrition from rare and unpredictable food falls. However, traits that allow organisms inhabiting the sea floor to exploit carrion are poorly understood. We found in laboratory experiments that hagfish (Eptatretus stouti), gastropods (Neptunea amianta) and amphipods (Orchomene obtusus) survived extended periods of starvation, in some cases for more than a year. When exposed to odors emitted from carrion, most individuals of E. stouti and O. obtusus began searching for food within seconds, whereas none responded to the scent of the live prey. In contrast, the slow crawling N. amianta readily consumed carrion but showed no apparent response to any odor solutions tested. Because more motile animals exhibited lower thresholds for response to signal molecules, sensitivity to chemical cues appears related to species mobility. Hagfish were also found to defend carrion from some competitors by releasing slime when feeding. Though varying dramatically in size, morphology, locomotive ability, and phylogeny, these three species all possess traits well suited for a scavenging lifestyle.

Populations of the articulate brachiopod Laqueus californianus occur in dense single-species aggregations near the continental shelf/slope break (100-200 m) in Monterey Bay, California. The development of embryos and larvae of L. californianus has been examined by scanning electron microscopy. Fertilizable eggs are 130-140 {mu}m in diameter, and sperm are unmodified. Cleavage is holoblastic and radial. At 10{deg}C an up-swimming blastula develops by 18-h, and gastrulation occurs within 24-38 h. The embryo elongates on a new larval axis and the blastopore closes by 72 h. A trilobed articulate brachiopod larva forms by day 3-4, and a metamorphically competent larva with attachment disk is attained in 7 days. Competent larvae swim downwards. Effects of temperature on larval survival and development rate have also been examined. Larvae die within 1 day at 25{deg}C. At 20{deg}C, development appears normal but results in spontaneous abnormal settlement of larvae 5-6 days old. At 15{deg}, 10{deg}, and 5{deg}C, most larvae achieve competence in 5, 7, and 9 days, respectively. Many larvae survive for 71 days at 10{deg} and 15{deg}C. Patterns of larval settlement vary among substrates, but larvae show strong preference for shells of living conspecific adults. Settlement and metamorphosis can occur within 24 h upon exposure of larvae to substrate.

© IWA Publishing 2014. With the maturation and certification of several ballast water management systems that employ chlorine as biocide to prevent the spread of invasive species, there is a clear need for accurate and reliable total residual oxidants (TRO) technology to monitor treatment dose and assure the environmental safety of treated water discharged from ships. In this study, instruments used to measure TRO in wastewater and drinking water applications were evaluated for their performance in scenarios mimicking a ballast water treatment application (e.g., diverse hold times, temperatures, and salinities). Parameters chosen for testing these technologies in the past do not reflect conditions expected during ballast water treatment. Salinity, temperature, and oxidant concentration all influenced the response of amperometric sensors. Oxidation reduction potential (ORP) sensors performed more consistently than amperometric sensors under different conditions but it may be difficult to correlate ORP and TRO measurements for the multitude of biogeochemical conditions found naturally in ballast water. N,N-diethyl-p-phenylenediamine (DPD) analyzers and amperometric sensors were also tested under intermittent sampling conditions mimicking a ballasting scenario, with cyclical dosage and discharge operations. When sampling was intermittent, amperometric sensors required excessive response and conditioning times, whereas DPD analyzers provided reasonable estimates of TRO under the ballasting scenario.

Oysters (Crassostrea virginica) are valuable subjects for investigations of basic mechanisms regulating suspension feeding on invertebrate larvae. In separate laboratory experiments, we introduced inert particles or larvae to the oyster mantle cavity. Small inert particles ( 100~pm diam) equal to larvae in size were rejected. Neither particle specific gravity nor phytoplankton in the seawater medium affected ingestion of inert particles or larvae. Oysters consumed a wide range of invertebrate larval forms, including conspecific veligers. Nearly all larvae (> 75%) delivered to the oyster mantle cavity, except those of Bug&a neritina, were eaten and digested. Feeding stimulants in the larvae were a mixture of polar and nonpolar compounds that were insoluble in seawater. Although contrasting mechanisms regulated fteding on different-sized particles, ingestion of larvae was strictly controlled by particle surface chemistry (=flavor).

Planktonic oyster larvae (Crassostreu virginica) respond behaviorally to waterborne chemical cues and rapidly settle on substratum. “Settlement” is defined here as attachment of the larval foot to the substrate. Although a topic of considerable research for the past 50 yr, the identity of these cues remains unresolved. We provide four lines of experimental evidence all pointing to the singular identity of these substances. First, molecular-weight fractionations of seawater used to bathe adult oysters (with intact biofilms) were bioassayed, indicating the presence of waterborne settlement inducers between 500 and 1,000 Da. The inducers were degraded by proteases but not by carbohydrases or by lipase. Of several proteases we applied, only those cleaving basic amino acids (lysine and arginine) from the C-terminal and arginase (an enzyme condensing arginine at the C-terminal to ornithine) eliminated settlement-inducing activity. Second, trypsin hydrolysates of casein were significantly more effective in causing larval settlement than products of either acid or pronase hydrolysis of this protein. Third, a tri-peptide having arginine at the C-terminal, glycyl-glycyl+arginine (hereafter referred to as GGR), evoked settlement at a concentration as low as lo-lo M. Dose-response curves for GGR and for the active fraction (500-1,000 Da) of oyster bath water were essentially identical. Fourth, tests of 21 free amino acids identified only lysine and arginine as settlement cues. Larval settlers were far more sensitive to arginine than lysine but significantly less sensitive to arginine than to peptides with arginine at the C-terminal. Our combined results are all consistent in identifying low-molecular-weight peptides with arginine at the C-terminal as the natural, water-soluble cues inducing oyster settlement.

There is an international commitment to develop a comprehensive, coordinated and sustained ocean observation system. However, a foundation for any observing, monitoring or research effort is effective and reliable in situ sensor technologies that accurately measure key environmental parameters. Ultimately, the data used for modelling efforts, management decisions and rapid responses to ocean hazards are only as good as the instruments that collect them. There is also a compelling need to develop and incorporate new or novel technologies to improve all aspects of existing observing systems and meet various emerging challenges. Assessment of Sensor Performance was a cross-cutting issues session at the international OceanSensors08 workshop in Warnemünde, Germany, which also has penetrated some of the papers published as a result of the workshop (Denuault, 2009; Kröger et al., 2009; Zielinski et al., 2009). The discussions were focused on how best to classify and validate the instruments required for effective and reliable ocean observations and research. The following is a summary of the discussions and conclusions drawn from this workshop, which specifically addresses the characterisation of sensor systems, technology readiness levels, verification of sensor performance and quality management of sensor systems.

The value of coastal and ocean observing is well documented. In particular, both the NOAA-led Integrated Ocean Observing System (IOOS) and the NSF-funded Ocean Observatories Initiative (OOI) have been established to address the management, safety, and scientific needs of the US and to better understand and predict changes to important ocean and coastal resources and processes. A foundation for successful ocean observing is access to, and implementation of, effective, reliable, and standardized sensors and sensor platforms to collect environmental data. The Alliance for Coastal Technologies (ACT) was therefore established by NOAA as partnership of research institutions, state and regional resource managers, and private sector companies interested in developing and applying sensors and sensor platforms for monitoring and studying aquatic systems. ACT goals include: transitioning emerging technologies to operational use rapidly and effectively; maintaining a dialogue among technology users, developers, and providers; identifying technology needs and novel technologies; documenting technology performance and potential; and providing IOOS with information required for the deployment of reliable and cost-effective networks. These goals are accomplished by providing three basic services: (1) a third-party tested for evaluating existing and developing sensor and sensor platform technologies, (2) a comprehensive data and information clearinghouse on environmental technologies, and (3) a forum for consensus and capacity building through a series of annual workshops on specific technology topics. This paper, and associate presentation, will review the ACT program, highlight accomplishments, and discuss trends found during the evaluations of in situ sensors.