9 results on '"Mario Arce-Montoya"'
Search Results
2. Alfalfa Plants (Medicago sativa L.) Expressing the 85B (MAP1609c) Antigen of Mycobacterium avium subsp. paratuberculosis Elicit Long-Lasting Immunity in Mice
- Author
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Carlos Angulo, Elizabeth Monreal-Escalante, Mario Arce-Montoya, Amalia León-Gallo, Virginie Roupie, Sergio Rosales-Mendoza, Cristhian Sández-Robledo, Kris Huygen, and Sawako Hori-Oshima
- Subjects
0106 biological sciences ,Antigenicity ,Plant-made vaccine ,medicine.medical_treatment ,Paratuberculosis ,Administration, Oral ,Bioengineering ,Biology ,85B antigen ,01 natural sciences ,Applied Microbiology and Biotechnology ,Biochemistry ,Microbiology ,Enteritis ,03 medical and health sciences ,Antigen ,Adjuvants, Immunologic ,Immunity ,010608 biotechnology ,medicine ,Oral vaccine ,Animals ,Molecular Biology ,030304 developmental biology ,0303 health sciences ,Original Paper ,Antigens, Bacterial ,Mice, Inbred BALB C ,Immunogenicity ,Alfalfa ,medicine.disease ,Plants, Genetically Modified ,Antibodies, Bacterial ,Mycobacterium avium subsp. paratuberculosis ,biology.protein ,Immunization ,Antibody ,Adjuvant ,Biotechnology ,Medicago sativa - Abstract
Mycobacterium avium subsp. paratuberculosis (MAP) is the etiological agent of Paratuberculosis, a contagious, untreatable, and chronic granulomatous enteritis that results in diarrhea, emaciation, and death in farmed ruminants (i.e., cattle, sheep, and goats). In this study, the Ag85B antigen from MAP was expressed in transgenic alfalfa as an attractive vaccine candidate. Agrobacterium-mediated transformation allowed the rescue of 56 putative transformed plants and transgenesis was confirmed in 19 lines by detection of the Ag85B gene (MAP1609c) by PCR. Line number 20 showed the highest Ag85B expression [840 ng Ag85B per gram of dry weight leaf tissue, 0.062% Total Soluble Protein (TSP)]. Antigenicity of the plant-made Ag85B was evidenced by its reactivity with a panel of sera from naturally MAP-infected animals, whereas immunogenicity was assessed in mice immunized by either oral or subcutaneous routes. The plant-made Ag85B antigen elicited humoral responses by the oral route when co-administered with cholera toxin as adjuvant; significant levels of anti-85B antibodies were induced in serum (IgG) and feces (IgA). Long-lasting immunity was evidenced at day 180 days post-first oral immunization. The obtained alfalfa lines expressing Ag85B constitute the first model of a plant-based vaccine targeting MAP. The initial immunogenicity assessment conducted in this study opens the path for a detailed characterization of the properties of this vaccine candidate. Supplementary Information The online version contains supplementary material available at 10.1007/s12033-021-00307-w.
- Published
- 2021
3. Infection of maize inbred B73 by Ustilago maydis and Fusarium proliferatum triggers differential expression of the β-glucosidase genes
- Author
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Gracia Gómez-Anduro, Mario Rojas, Axel Tiessen, Felipe Ascencio, and Mario Arce-Montoya
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0106 biological sciences ,0301 basic medicine ,Oryza sativa ,biology ,Ustilago ,Defence mechanisms ,food and beverages ,Fusarium proliferatum ,Plant Science ,Biotic stress ,biology.organism_classification ,01 natural sciences ,Microbiology ,03 medical and health sciences ,030104 developmental biology ,Gene expression ,Genetics ,Arabidopsis thaliana ,Gene ,010606 plant biology & botany - Abstract
β-glucosidases participate (EC 3.2.1.21) in the activation of glycosylated and inactive forms of various compounds during plant response to the environmental conditions. The understanding of regulatory mechanisms for β-glucosidases in each plant species and their functionality is the purpose of various investigations. Our study reports the expression analysis of 10 β-glucosidase genes in corn seedlings after a challenge infection ith Fusarium proliferatum and Ustilago maydis compared with the gene expression analysis after mechanical damage and without damage to the seedlings under in vitro culture conditions. Likewise, the heterologous sequence comparison of the β-glucosidases from Zea mays and the characterized β-glucosidases of Oryza sativa and Arabidopsis thaliana were reported. Our results suggest a potential similitude in the functionality of this group of β-glucosidase genes in maize during the response to biotic stress and the mechanical damage that also showed possible tissue-specific regulation. Our results suggest the transcription regulation of these β-glucosidases and their possible influence in the defence mechanism against fungal infections in maize.
- Published
- 2018
4. Antiporter NHX2 differentially induced in Mesembryanthemum crystallinum natural genetic variant under salt stress
- Author
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Mario Rojas, Mario Arce-Montoya, Andrés Orduño, Claudia Villicaña, Carlos Angulo, Norberto Warner, and Gracia Gómez-Anduro
- Subjects
0106 biological sciences ,0301 basic medicine ,Genetics ,Antiporter ,Mesembryanthemum crystallinum ,Wild type ,Plant physiology ,Horticulture ,Biology ,biology.organism_classification ,01 natural sciences ,Salinity ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,chemistry ,Chlorophyll ,Gene expression ,Botany ,Gene ,010606 plant biology & botany - Abstract
Plants exhibit several mechanisms to survive under high salinity conditions. The uptake and compartmentalization of Na+ ion by the NHX antiporter is a crucial mechanism in homeostasis maintenance. Therefore, we evaluated McNHX2 gene expression and several physiological responses induced in three natural genetic variants of ice plants under salt stress. Based on morphology and growth behavior of wild type populations from an arid region of northwestern Mexico, we identified three ice plant natural genetic variants and called P0, P9, and P11. Several physiological parameters, such as water potential, relative water content, chlorophyll, and Na+ and K+ ion contents from all natural genetic variants exhibited a differential response under high salinity conditions. Specifically, the P0 variant showed lower water potential changes and least perturbation of Na+/K+ ratio than those of the P9 and P11 variants under saline conditions, suggesting that the P0 variant is the most salt tolerant. Unexpectedly, McNHX2 expression was repressed in the P11 variant while it was upregulated in the P0 and P9 variants under saline treatments. The McNHX2 gene was sequenced showing 15 introns and 16 exons; polymorphisms were found among the cDNAs sequences from the three natural genetic variants. All these data suggest that differential responses to high salinity involve different mechanisms operating in each variant for counteracting saline stress effects.
- Published
- 2015
5. No correlation between the growth of in vitro cultured Yucca valida clones and the growth of their mother plants in the field
- Author
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Manuel L. Robert, Mario Arce-Montoya, Margarito Rodríguez-Álvarez, and Julio Hernandez-Gonzalez
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Cloning ,Horticulture ,biology ,Micropropagation ,Dry weight ,Yucca schidigera ,Botany ,Yucca ,Plant physiology ,Yucca valida ,Domestication ,biology.organism_classification - Abstract
Yucca valida is a close relative to Yucca schidigera and a potential alternative source of the steroidal saponins extracted from the tissues of the latter. The exploitation from the wild is not only inefficient but endangers the natural populations and an alternative to these would be their domestication and establishment of commercial plantations. Both species have a long life span cycle and low growth rates affected by environmental conditions that make it difficult to select fast growing, high yield elite individuals for cloning purposes. Here, we report the in vitro establishment and propagation of mature Y. valida plants derived from seed that showed very different growth rates in an experimental field and their propagative and growth characteristics in vitro. No correlation was found between the growth of field cultivated plants and the relative increase of fresh (FW) or dry weight (DW) of in vitro cultured plants.
- Published
- 2006
6. Differential responses of Chiltepin (Capsicum annuum var. glabriusculum) and Poblano (Capsicum annuum var. annuum) hot peppers to salinity at the plantlet stage
- Author
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Gracia A. Gomez, uro, Diana Medina-Hernández, Raul Lopez-Aguilar, Juan A. Larrinaga-Mayoral, Mario Arce-Montoya, Alej, EnriqueTroyo-Dieguez, ra Nieto-Garibay, and Felipe Ascencio-Valle
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Chlorophyll content ,food and beverages ,Hot peppers ,Biology ,Applied Microbiology and Biotechnology ,Salinity ,Plantlet ,Capsicum annuum ,Dry weight ,Botany ,Pepper ,Genetics ,Agronomy and Crop Science ,Molecular Biology ,Biotechnology ,Transpiration - Abstract
Poblano (Capsicum annuum var. annuum) and Chiltepin pepper (Capsicum annuum var. glabriusculum) are considered closely related parent species. Poblano pepper is a cultivated species that has lost stress tolerance attributes, and Chiltepin is a wild species adapted to adverse environmental conditions, such as salinity stress. Differential physiological responses between the wild and cultivated species were compared in order to study the physiological and biochemical mechanisms of Chiltepin’s salinity tolerance. Salt stress treatments were applied to Chiltepin and Poblano and their growth, chlorophyll content, transpiration rate, and concentrations of anions and cations in leaves, stem and roots were measured. Dry weight and relative growth index decreased significantly with salt stress in Poblano, chlorophyll-a decreased significantly in both peppers and transpiration decreased in Chiltepin, with no significant changes evidenced by Poblano. Concentrations of Na
- Published
- 2012
7. Composición del aceite esencial de Tagetes lacera, planta endémica de Baja California Sur, México
- Author
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Francisco Díaz-Cedillo, Miguel Ángel Serrato-Cruz, Mario Arce-Montoya, and José Luis León de la Luz
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Botánica ,Biología ,planta endémica ,composición del aceite esencial ,Biology ,steraceae ,Humanities ,Ecology, Evolution, Behavior and Systematics - Abstract
"Es escasa la información sobre la biología y química de Tagetes lacera Brand. (Asteraceae), especie endémica de Baja California Sur (BCS), México, que por su porte alto y presencia de aroma es una fuente de aceites esenciales útil para el control de plagas y enfermedades de cultivos agrícolas. A partir de partes aéreas de plantas en floración de T. lacera recolectadas en la sierra de la Laguna, BCS se obtuvo aceite esencial mediante hidrodestilación. Por medio del procedimiento de análisis CG-EM, se identificaron 6 compuestos principales: E-tagetona (26.2%), crisantenona (24.8%), verbenona (22.1%), a-thujeno (20.5%), B-pineno (3.1%) y a-pineno (1.9%)."
- Published
- 2012
8. No correlation between the growth of in vitro cultured Yucca valida clones and the growth of their mother plants in the field.
- Author
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Mario Arce-Montoya, Julio Hernández-González, and Margarito Rodríguez-Álvarez
- Abstract
Abstract??Yucca validais a close relative toYucca schidigeraand a potential alternative source of the steroidal saponins extracted from the tissues of the latter. The exploitation from the wild is not only inefficient but endangers the natural populations and an alternative to these would be their domestication and establishment of commercial plantations. Both species have a long life span cycle and low growth rates affected by environmental conditions that make it difficult to select fast growing, high yield elite individuals for cloning purposes. Here, we report the in vitro establishment and propagation of matureY. validaplants derived from seed that showed very different growth rates in an experimental field and their propagative and growth characteristics in vitro. No correlation was found between the growth of field cultivated plants and the relative increase of fresh (FW) or dry weight (DW) of in vitro cultured plants. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
9. Micropropagation and field performance of Yucca valida.
- Author
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Mario Arce-Montoya, Margarito Rodríguez-Álvarez, Julio Hernández-González, and Manuel Robert
- Abstract
Yucca valida is an important potential source of steroidal saponins closely related to Yucca schidigera, the species that is commercially exploited from the wild as a source of steroidal extracts. Neither of the species has been domesticated mainly because of their slow growth and long life span before harvesting. Here, we report a micropropagation method to generate isogenic or clonal lines for plantation purposes. Seventeen clonal lines were propagated and evaluated over a period of 26 months in an experimental plantation and compared with the performance of plants from seeds. The large variability found between the plants derived from seeds is manifested in the differences observed between the different clonal lines; however, these present a much smaller internal coefficient of variation than the one observed in the population of plants derived from seeds. Some clonal lines perform in a superior manner indicating that a process of selection and cloning can generate lines of fast growing individuals for plantations that can satisfy the demand for these materials without putting a natural resource at risk. [ABSTRACT FROM AUTHOR]
- Published
- 2006
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