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Study this intended to analyze and describe productive zakat management to the well-being of Mustahik at Baznaz Gorontalo Regency. Problem-related to well-being Mustahik is interesting for research because Welfare is a condition that is the hope of every citizen. However, not all citizens quickly get Welfare, especially Mustahik. This study used a qualitative approach with descriptive analysis and data collection techniques using observation, interviews, and documentation. The results from the survey are organizing zakat BAZNAS Gorontalo Regency, namely by forming sub-sectors to support operational running management zakat, namely the zakat collection unit (UPZ), which is responsible for collecting zakat from muzak and implementing it as in the distribution and utilization of productive zakat in management, namely the distribution is uneven because the people in Gorontalo Regency have different economic conditions. Novelty in this research is that we found Supervision in the direction of zakat is carried out by forming an internal audit unit to oversee the work system implemented by each implementing area.

Pengadaan tanah skala besar Yogyakarta International Airport (YIA) membawa pengaruh terhadap aspek fisik, lingkungan, sosial, ekonomi maupun budaya. Kajian ini bertujuan melakukan pemetaan perubahan penggunaan lahan yang terjadi akibat pembangunan bandara YIA. Metode penelitian dilakukan secara spasial, penggunaan lahan diperoleh melalui interpretasi citra melalui pendekatan NDVI (Normalized Difference Vegetation Index). Data yang digunakan untuk monitoring pola perubahan penggunaan lahan ialah citra satelit Sentinel-2 Tahun 2015 dan 2019. Hasil kajian menunjukkan perubahan penggunaan lahan di Kecamatan Temon, Pengasih, dan Wates, perubahan tertinggi terjadi pada kebun campur hingga mencapai luasan 910 Ha, sementara perubahan lahan sawah mencapai 289,44 Ha. Tingginya alih fungsi lahan pertanian ini dapat berimplikasi terhadap transformasi matapencaharian penduduk yang sebelumnya menggantungkan hidup pada sektor agraris. Monitoring perubahan penggunaan lahan yang dilakukan berkala harapannya menjadi dasar dalam melakukan pengendalian pertanahan dan pemanfaatan ruang.

Natural killer (NK) cells are innate immune cells that can remove viral-infected tumour cells without antigen priming. This characteristic offers NK cells an edge over other immune cells as a potential therapy for nasopharyngeal carcinoma (NPC). In this study, we report how cytotoxicity was evaluated in target NPC cell lines and patient-derived xenograft (PDX) cells with effector NK-92, a commercially available NK cell line, by using xCELLigence RTCA system (a real-time, label-free impedance-based monitoring platform). Cell viability, proliferation and cytotoxicity were examined by RTCA. Cell morphology, growth and cytotoxicity were also monitored by microscopy. RTCA and microscopy showed that both target and effector cells were able to proliferate normally and to maintain original morphology in co-culture medium as they were in their own respective culture medium. As target and effector (T:E) cell ratios increased, cell viability as measured by arbitrary cell index (CI) values in RTCA decreased in all cell lines and PDX cells. NPC PDX cells were more sensitive to the cytotoxicity effect of NK-92 cells, than the NPC cell lines. These data were substantiated by GFP-based microscopy. We have shown how the RTCA system can be used for a high throughput screening of the effects of NK cells in cancer studies to obtain data such as cell viability, proliferation and cytotoxicity.

Pasar terapung yang melegenda dan memiliki keindahan-keindahan alam yang eksotis, asri dan alami, seolah menjadikan sungai sebagai warisan budaya masyarakat dalam mencari makna hidup. Pasar tradisional yang mungkin satu-satunya di Indonesia ini menjadi salah satu tempat wisata andalan di kawasan Banjarmasin, Kalimantan Selatan. Penelitian ini bertujuan untuk mengkaji dan mencari fenomena alam dan hambatan dalam membangun pusaka harmonisasi masyarakat Banjar untuk mempertahankan nilai-nilai internalisasi budaya dalam perspektif Pendidikan Kewarganegaraan berbasis kearifan lokal budaya sungai. Penelitian ini menggunakan pendekatan kualitatif melalui metode penelitian fenomenologis: observasi langsung, wawancara, dokumentasi, dan meringkas penyelesaian pekerjaan di lapangan. Hasil penelitian ini mengungkapkan hal-hal sebagai berikut: faktor penghambat perkembangan cagar budaya sungai pada masyarakat Banjar dengan arus globalisasi dan modernisasi saat ini meghasilkan cara hidup generasi muda menjadi materialistis pragmatis, sehingga gaya hidup sangat konsumtif. Namun, pemerintah daerah, masyarakat Banjar dan akademisi terus berupaya untuk mendukung dan menjunjung tinggi nilai-nilai luhur internalisasi, dengan kewajiban untuk melibatkan generasi muda masyarakat dalam menciptakan dan memelihara budaya daerah melalui pasar terapung. Keberadaan pasar terapung yang didirikan oleh Siring Tendean di kota Banjarmasin, yang tetap menjunjung tinggi nilai-nilai gotong royong, kerja sama, kerja keras, tolong-menolong, kekeluargaan, dan empati, menghasilkan nilai-nilai sosial dalam pembentukan masyarakat madani, budaya dalam masyarakat, dan penguatan ekonomi nasional dengan bantuan ikon wisata sungai.

Persoalan anak terlantar masih menjadi persoalan serius di Indonesia. Berdasarkan data Kementerian Sosial yang diambil dari Data Terpadu Kesejahteraan Sosial (DTKS) SIKS-NG per-15 Desember 2020, jumlah anak terlantar di Indonesia sebanyak 67.368 orang. Angka tersebut bisa jadi lebih tinggi karena tidak semua anak yang terlantar datanya terekam dengan baik. Permasalahan IMM Allende yang menjadi mitra dalam kegiatan pengabdian diantaranya; SDM yang kurang memiliki karakter tangguh dalam melakukan kerja sosial di lapangan, kurangnya penguasaan dalam melakukan pendekatan kepada anak-anak jalanan yang menjadi sasaran binaan. Sedangkan permasalahan binaan mitra yakni motivasi belajar rendah, dan terbatasnya waktu belajar karena harus bekerja. Tujuan kegiatan pengabdian ini pertama untuk membantu penguatan organisasi dan SDM mitra agar memiliki kesiapan pendampingan pada anak-anak jalanan, membantu membangun penguatan hubungan antara mitra dan anak binaan serta menciptakan pembelajaran yang menarik dalam proses pendampingan. Metode penanganan pada kegiatan PKM ini adalah metode Street Based. Hasil dari pengabdian ini, anak-anak yang menjadi sasaran mitra terpenuhi haknya mendapatkan layanan pendidikan. Mereka antusias dan mengalami peningkatan kemampuan hasil belajar. Sedangkan bagi relawan IMM Allende, kegiatan pengabdian ini mampu membangun kesadaran pentingnya komunikasi dan koordinasi dalam organisasi sehingga melahirkan kekompakan dan ketangguhan menjadi relawan sosial.

The development of information technology which is supported by the rapid use of the internet has created several fintech that will make it easier for people to get digital financial services. The Financial Services Authority stated that the increase in national financial inclusion could be triggered by one factor, namely increased access to the use of fintech. The purpose of this study aimed to determine the role of fintech on financial inclusion in MSMEs in South Tangerang. The population in this study are MSMEs in South Tangerang with a sample of 487 informants. Researchers used primary data in the form of questionnaires that were distributed online and offline. The convenience sampling method is used in data collection in this type of quantitative research. Linear regression was used as an analytical technique. This study concluded that fintech has a positive effect on financial inclusion by 9%. ABSTRAK Perkembangan teknologi informasi yang didukung dengan pesatnya penggunaan internet memunculkan beberapa fintech yang akan mempermudah masyarakat untuk mendapatkan layanan keuangan secara digital. Otoritas Jasa Keuangan menyatakan bahwa peningkatan inklusi keuangan nasional dapat dipicu oleh salah satu faktor yaitu peningkatan akses dalam penggunaan fintech. Tujuan penelitian ini adalah untuk mengetahui peran fintech terhadap inklusi keuangan pada UMKM se Tangerang Selatan. Populasi dalam penelitian ini adalah pelaku UMKM di wilayah Tangerang Selatan dengan sampel sebanyak 487 informan. Peneliti menggunakan data primer berupa kuesioner yang disebar secara online dan offline. Penelitian ini merupakan jenis penelitian kuantitatif yang menggunakan metode convenience sampling dalam pengumpulan data. Riset ini menggunakan regresi linier untuk menganalisis data. Penelitian ini menghasilkan kesimpulan bahwa fintech memiliki pengaruh yang positif terhadap inklusi keuangan sebesar 9%.

BPJS Kesehatan selama ini belum memanfaatkan teknologi informasi secara maksimal dalam mendukung proses bisnisnya. Proses bisnis yang belum dilakukkan secara optimal antara lain proses pendaftaran bayi baru lahir, proses konfirmasi kepesertaan dan proses cetak kartu sementara bayi baru lahir. Hal ini disebabkan karena masih ada ketidaksamaan antara arsitektur bisnis, arsitektur sistem informasi dan arsitekture teknologi informasi.Permasalahan tersebut mengakibatkan pelayanan terhadap peserta BPJS Kesehatan tidak efisien. Adapun tujuan yang akan dicapai yaitu menghasilkan blueprint enterprise architecture Sistem Pelayanan BPJS Kesehatan berbasis web dengan framework TOGAF. Penelitian ini menggunakan kerangka kerja TOGAF dengan metode ADM (Architecture Development Method) berdasarkan data kualitatif yang didapat dari hasil wawancara. Hasil akhir dari penelitian ini berupa blueprint enterprise architecture yang diharapkan dapat mempermudah dan mengintegrasikan proses bisnis pelayanan pada BPJS Kesehatan Cabang Kendal. Kata kunci: TOGAF, Enterprise Architecture, Blueprint, Sistem Pelayanan BPJS Kesehatan, Ptototype System

Yellow root (Arcangelisia flava (L.) Merr.) is a medicinal plant used traditionally in Borneo, Indonesia, for several diseases. Nevertheless, it contains berberine alkaloids that are considered harmful. Empirically, based on local wisdom people do not boil yellow roots with water but with brackish water. This study aimed to determine the effects of yellow root decoctions made using water and brackish water on blood biochemical and histopathological profile of kidneys and uterus in Wistar rats after subchronic treatment. Overall, 70 Wistar female rats were divided into seven groups of which three groups were given yellow root water decoctions and three groups were given yellow root brackish water decoctions with doses equivalent to 1.25 g/kg BW, 2.5 g/kg BW and 5 g/kg BW of dried yellow root, whereas the control group was only given Aquadest. After 28 days of treatments, blood samples were obtained for biochemical examination followed by a surgical examination to observe the histological features of the organs. The results revealed significant differences in the blood urea nitrogen (BUN) levels between group that was given water decoction of the yellow root and the control group. On the other hand, the groups that were given brackish water decoction revealed no significant changes, except at the highest dose. Furthermore, histopathological features of rats treated using all doses of the yellow root water decoctions revealed organ damage compared with the normal group, manifested as hemorrhage, degeneration, and necrosis of the kidneys, as well as epithelial damage and inflammatory infiltration of the uterus.

A recent challenge in bioimaging is the observation and imaging of vital, thick, and complex tissues in real time and in non-invasive mode. In the last decade, non-linear excitation microscopy showed several advantages for in-vivo imaging compared to conventional confocal techniques. Nevertheless, deep tissue imaging remains challenging, especially for thick media, due to spherical aberrations induced on focused beams by the tissue. A low numerical aperture objective lens coupled to high dioptric power microlenses, implanted in the tissue, can be beneficial for the reduction of optical aberrations. In this context, we fabricated a system of plano-convex microlenses and microscaffolds on a single chip by means of two-photon polymerization), to be used for non-linear imaging of biological specimens.

In everyday life, humans often encounter problems in decision making, where problems that arise can be large or small, which are very influential in the results of decisions. Now humans are developing systems that can help determine the best alternative in a problem, namely the decision support system (DSS) in the decision support system there are alternatives, criteria and weights used to determine the best solution. The quality of human resources is one of the supporting factors to improve the performance productivity of an agency. So, from that competent human resources can support the level of performance, with performance appraisal, it will be known the achievements of each employee, this can be used by agencies as a consideration in determining the best employees. The purpose of this study is to create a good decision support system that can assist managers in evaluating the performance of an employee in an agency. Judging from the problem of the performance appraisal of an employee at PT. Cahaya iqra Mandiri is the object of research that produces the desired answer, the Admin section is a priority and can be implemented to determine neat administration and have important responsibilities in PT. cahaya iqra Mandiri.

Proses pengelolaan nilai pada SMP Negeri 10 pangkalpinang ini masih belum terkomputerisasi sehingga banyak mengalami keterlambatan dalam penyajian data terutama dalam data siswa, data nilai, rekap nilai, raport dan laporan. Untuk itu penulis mencoba mengatasi dengan melakukan pengendalian sistem pengolahan nilai dengan cara membuat sistem terkomputerisasi yaitu sistem pengelolaan nilai dengan tujuan supaya mengefisienkan dari segi waktu dan biaya. Adapun hasil sistem usulah yang ingin dicapai dengan adanya sistem terkomputerisasi ini supaya mempermudah dalam pengolahan data, pencarian data nilai, penyimpanan data dan mempelajari tata cara pengolahan data yang lebih efisien, dan mengimplementasikan sistem pengolahan data nilai. Metode yang digunakan dalam tahap pengolahan data nilai ini adalah menggunakan konsep UML, pengelolaan data base menggunakan MQSL. Dalam datahap perancangan menggunakan pemrograman visual versi 8.

Abstrak - Akademi Abdi Nusa pangkalpinang merupakan sistem informasi yang bergerak dibidang pendidikan kesehatan. Dengan adanya sistem interprise ini maka akan mendukung perencanaan sistem informasi yang baik dan teratur. Perencanaan sistem informasi yang masih kurang baik mengakibatlan kurang terarah prosedur sistem informasi pada Akademi tersebut. Maka dari itu penulis akan merancang sistem blue print pada Akademi. Sistem enterprise Architecture Planning (EAP) yaitu penataan operasi pengertian dari penataankepada pemakian informasi untuk menunjang kegiatan, penataan untuk penerapan penataan sistem. Dipaper ini memaparkan tentang penataan arsitektur informasi pada organisasi, Akademi, sistem informasi ini saling berhubungan antar bagian supaya untuk mempermudah dalam mendapatkan informasi. Model yang dibangun ini bertujuan diharapkan pengembangan sistem informasi dimasa yang akan datang. Penelitian ini penulis akan mengkaji penerapan model sistem informasi architecture Planning pada Akbid Abdi Nusa pangkalpinang dengan menggunakan konsep blue print diantaranya architectur data,aplikasi dan teknologi. sistem informasi Akademik ini diawali tahap menganalisa kegiatan akademik dan strategi kegiatan pendidikan Kata kunci— Sistem, Informasi, Sistem Interprise, Architectur, Blue Print

Surface active agents (SAAs), currently used in modern industry, are synthetic chemicals produced from non-renewable sources, with potential toxic impacts on humans and the environment. Thus, there is an increased interest for the identification and utilization of natural derived SAAs. As such, the marine environment is considered a promising source of biosurfactants with low toxicity, environmental compatibility, and biodegradation compared to their synthetic counterparts. MARISURF is a Horizon 2020 EU-funded project aiming to identify and functionally characterize SAAs, derived from a unique marine bacterial collection, towards commercial exploitation. Specifically, rhamnolipids produced by Marinobacter MCTG107b and Pseudomonas MCTG214(3b1) strains were previously identified and characterized while currently their toxicity profile was assessed by utilizing well-established methodologies. Our results showed a lack of cytotoxicity in in vitro models of human skin and liver as indicated by alamar blue and propidium iodide assays. Additionally, the use of the single gel electrophoresis assay, under oxidative stress conditions, revealed absence of any significant mutagenic/anti-mutagenic potential. Finally, both 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonicacid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) cell-free assays, revealed no significant anti-oxidant capacity for neither of the tested compounds. Consequently, the absence of significant cytotoxicity and/or mutagenicity justifies their commercial exploitation and potential development into industrial end-user applications as natural and environmentally friendly biosurfactants.

The purpose of this study is to describe the learning process with the approach of Realistic Mathematics Education (RME), which can increase the motivation to learn mathematics student in materials Systems of Linear Equations in Two Variables on VIII-C students of SMP IT Ash-Shadhili. This research was a Classroom Action Research consisting of four stages, namely planning, action, observation and reflection. In the RME approach, teachers initiate learning by giving realistic problems. Students are asked to understand the problem and then solve it by discussing based on their knowledge and experience on teacher guidance. Students are then asked to discuss and compare answers through presentation activities during class discussions. Teachers motivate students to ask questions, give feedback or feedback. At the end of the learning the students are given the opportunity to deduce the material they have learned. The results showed that the RME approach learning can increase student motivation. This was proven by the percentage of observation results of students' learning motivation classically reached 83.33%, while the average percentage of observation results every meeting of two observers reached 80.37% in either category. Tujuan penelitian ini adalah untuk mendeskripsikan proses pembelajaran dengan pendekatan Realistic Mathematics Education (RME) yang dapat meningkatkan motivasi belajar matematika siswa pada materi Sistem Persamaan Linier Dua Variabel di kelas VIII-C SMP IT Asy-Syadzili. Penelitian ini merupakan Penelitian Tindakan Kelas yang terdiri atas empat tahapan, yaitu perencanaan, tindakan, pengamatan dan refleksi. Pada pendekatan RME, guru mengawali pembelajaran dengan memberikan masalah realistik. Siswa diminta untuk memahami masalah kemudian menyelesaikannya dengan cara berdiskusi berdasarkan pengetahuan dan pengalaman mereka atas bimbingan guru. Selanjutnya siswa diminta untuk mendiskusikan dan membandingkan jawaban melalui kegiatan presentasi pada saat diskusi kelas. Guru memotivasi siswa untuk berani bertanya, memberikan masukan atau tanggapan. Diakhir pembelajaran siswa diberi kesempatan untuk menyimpulkan materi yang telah dipelajari. Hasil penelitian menunjukkan bahwa pembelajaran dengan pendekatan RME dapat meningkatkan motivasi belajar siswa. Hal ini dibuktikan dengan persentase hasil observasi motivasi belajar siswa secara klasikal mencapai 83,33%, sedangkan rata-rata persentase hasil observasi setiap pertemuan dari dua orang observer mencapai 80,37% pada kategori baik.

This study details the reactive flow simulations of a LOX/CH4 Multi-element rocket engine. The work has been conducted within the framework of the HYPROB-BREAD project whose main objective is the design, manufacture and testing of a LOX/LCH4 regeneratively cooled ground demonstrator. Numerical simulations have been carried out with both commercial software and CIRA software developed in house. Two sets of boundary conditions, nominal and experimental, have been applied from which a code-to-code validation has been effected with the former and a code-to-experiment validation with the latter. The results presented include both flow data and heat fluxes as well as parameters associated with engine performance, and indicate an excellent agreement with experimental data of a LOX/CH4 Multi-element rocket engine.

Introduction: In the past decades, human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) have sparked interest in cellular therapy due to their immunomodulatory properties. Nevertheless, the fate of hUC-MSCs in the body remains poorly understood. This study aimed to investigate the biodistribution, homing and clearance of systemically administered hUC-MSCs in healthy BALB/c mice model. Methods: hUC-MSCs were labelled with GFP-Luc2 protein, followed by characterisation with flow cytometry. Upon intravenous infusion of transduced hUC-MSCs into the healthy BALB/c mice, the cells were dynamically monitored through the bioluminescent imaging (BLI) approach. Results: Transduction of hUC-MSCs with GFP-Luc2 not only preserved the characteristics of MSCs, but also allowed live monitoring of transduced cells in the mice model. Upon systemic administration, BLI showed that transduced hUC-MSCs first localised predominantly in the lungs of healthy BALB/c mice and mainly remained in the lungs for up to 3 days before eventually cleared from the body. At terminal sacrifice, plasma chemistry biomarkers remained unchanged except for C-peptide levels, which were significantly reduced in the hUC-MSCs group. Histopathological findings further revealed that hUC-MSCs infusion did not cause any adverse effects and toxicity to lung, liver and heart tissues. Conclusions: Collectively, systemically administrated hUC-MSCs was safe and demonstrated dynamic homing capacity before eventually disappearing from the body.

This research aims to develop a training model for basic hammer throwing techniques at the age of 13-15 years and determine the effectiveness of the training model that has been developed. The problem that is the basis for conducting this research is that several literatures have not found a hammer throwing training model for 13-15 year olds in DKI Jakarta Province using an e-book basis. The method used in this research is Borg and Gall research and development through 10 development steps. Participants involved in the small group test were 15 athletes in DKI Jakarta Province and in the large group test 32 athletes. Then the participants involved in testing the effectiveness of the model were 32 athletes who were divided into 2, namely 16 athletes in the control group and 16 athletes in the experimental group. Based on the results of the N-Gain Score (%) test calculation, it shows that the average value of the N-Gain Score for the experimental group is 81.4321 or 81.43%, which is included in the effective category. Meanwhile, the average N-Gain Score for the control group was 34.88 or 34.88%, which was included in the Ineffective category. From the results of these calculations it can be seen that the use of the training model in the experimental group was effective in improving basic hammer throwing technique skills.

The purpose of the article related to public involvement in budget disclosure, known as participatory budgeting, shows more in terms of the opportunities and challenges faced by a decentralized country, where actors in it participate in making decisions through budgeting formulation. This issue is focused on budget transparency not only to encourage public involvement, but public involvement is very necessary for budget transparency. However, this public engagement has its challenges and benefits. This analysis is carried out through literature studies, journals, and related publications. The data were collected through SCOPUS a qualitative. This study concludes that there are several challenges that need to be considered in implementing public involvement in budgeting. Overly complex budgets and inadequate knowledge make this public disinterest a challenge. A conflict occurs when many groups with different interests take part in it. Public involvement is supported because it can increase tax revenue collection and satisfaction in public services and other benefits for social welfare. The results of this analysis will show several challenges and opportunities that use public involvement to be successful in budget formulation so that reforms in the budget can be carried out and can be used as a model for other regions and can become a system of reform in budgeting.

Non-linear excitation microscopy offers superior in-vivo imaging but faces challenges in deep tissue. High numerical aperture beams suffer spherical aberrations, while tissue scattering impacts image quality. To address this, we propose implantable microlenses for precise focusing below the skin in lab animals. By using low numerical aperture lasers, we avoid spherical aberrations induced by high NA objectives. Our study presents various microlens designs differing in size, shape, and fabrication methods, all on glass or organo-hybrid ceramic substrates. This approach shows promise for enhancing deep tissue imaging, facilitating better understanding of biological processes in vivo.

Energy consumption in large building complexes has a specific profile, consisting of thermal energy and electricity. Due to the recent energy crises, operational costs of these needs have been significantly increased. According to EU Energies Policies, the future objective is the transition to renewable-based energy, and self-sufficient and nearly zero buildings. This paper investigates the electricity consumption profile of a specific hotel located in Crete. An algorithm has been introduced to maximize the self-sufficiency of the examined hotel. The algorithm succeeds in increasing the self-sufficiency of the hotel’s energy system up to 99% by determining the PV power and battery capacity.

Tersedianya Layanan SPAB (Sarana Penyedia Air Bersih) merupakan salah satu usaha yang dimiliki oleh BUMDes di desa - desa. Pada layanan SPAB ini masih dihadapkan pada beberapa permasalahan yang cukup kompleks dan masih belum diatasi sepenuhnya. Terutama dalam info tentang layanan air bersih, kendalanya karena kurangnya sosialisai pelayanan tersebut. Oleh karena itu pada penelitian ini akan memberikan informasi tentang Sistem informasi Layanan SPAB( sarana Penyedia Air Bersih) berbasis Web. Untuk mengatasi masalah tersebut maka dibutuhkan pembaruan sistem yang terintegrasi database agar proses pencatatan seperti data pelanggan, dan data pengguna lebih efektif. Metode yang digunakan dalam penelitian ini menggunakan System Developmet Life Cycle (SDLC) dan model penelitian yang digunakan adalah Metode Rapid Application Development (RAD) serta alat bantu analisa dan desain sistem menggunakan Unified Modelling Language (UML) dan menggunakan Basis Data. Hasil yang diharapkan adalah meningkatkan tagihan pelanggan bisa dilakukan petugas dengan efisien demi meningkatkan pelayanan terhadap pelanggan dan memudahkan pelanggan dalam memperoleh informasi tagihan SPAB (Sarana Penyedia Air Bersih) bagi masyarakat. Dengan adanya Pengembangan Sistem Layanan SPAB (Sarana Penyedia Air Bersih) berbasis Web maka proses pendaftaran hingga pengaduan pelanggan dapat dilakukan dengan efektif karena dilakukan secara online dan pelanggan pun bisa melihat informasi tagihannya di website. Dengan adanya sistem ini dapat mempermudah pelanggan dalam melakukan proses sehingga proses pelayanan jasa lebih mudah dan fleksibel dari segi waktu dan tempat.

The existence of unbounded solutions and their asymptotic behavior is studied for higher order differential equations considered as perturbations of certain linear differential equations. In particular, the existence of solutions with polynomial-like or noninteger power-law asymptotic behavior is proved. These results give a relation between solutions to nonlinear and corresponding linear equations, which can be interpreted, roughly speaking, as an asymptotic proximity between the linear case and the nonlinear one. Our approach is based on the induction method, an iterative process and suitable estimates for solutions to the linear equation.

Super-resolution image acquisition has turned photo-activated far-infrared thermal imaging into a promising tool for the characterization of biological tissues. By the sub-diffraction localization of sparse temperature increments primed by the sample absorption of modulated focused laser light, the distribution of (endogenous or exogenous) photo-thermal biomarkers can be reconstructed at tunable ∼10−50 μm resolution. We focus here on the theoretical modeling of laser-primed temperature variations and provide the guidelines to convert super-resolved temperature-based images into quantitative maps of the absolute molar concentration of photo-thermal probes. We start from camera-based temperature detection via Stefan–Boltzmann’s law, and elucidate the interplay of the camera point-spread-function and pixelated sensor size with the excitation beam waist in defining the amplitude of the measured temperature variations. This can be accomplished by the numerical solution of the three-dimensional heat equation in the presence of modulated laser illumination on the sample, which is characterized in terms of thermal diffusivity, conductivity, thickness, and concentration of photo-thermal species. We apply our data-analysis protocol to murine B16 melanoma biopsies, where melanin is mapped and quantified in label-free configuration at sub-diffraction 40 µm resolution. Our results, validated by an unsupervised machine-learning analysis of hematoxylin-and-eosin images of the same sections, suggest potential impact of super-resolved thermography in complementing standard histopathological analyses of melanocytic lesions.

The EU has set ambitious targets to combat climate change. Incorporating renewable energy technologies to reduce greenhouse gas emissions is a critical aspect of achieving the European Union’s (EU) 2030 climate goals. Similarly to all member countries of the EU, Greece shares the same climate goals. In order to achieve these goals, ensuring a consistent supply and the effective use of clean energy is pursued, as it has a significant impact on the sustainable development and growth of the country. As the Greek tourism sector is one of the most energy-consuming of the national economy and a major contributor to the country’s GDP, opportunities are presented for innovation and investment in sustainable practices. Such investments must focus on buildings and facilities, where the energy consumption is concentrated. One of the most popular holiday destinations in Greece is the island of Crete. Visitation patterns are seasonal, which means during the summer months, Crete is exceptionally popular and more demanding energy-wise. One of the highest energy-demanding types of tourism-based businesses is the hospitality industry. Energy demands in hotels are driven by factors such as heating, cooling, lighting, and hot water. Thus, such activities require thermal and electrical energy to function. Electrical energy is one of the most essential forms of energy for hotels, as it powers a wide range of critical systems and services throughout the establishment. Therefore, the hotels are highly susceptible to fluctuations in energy prices which can significantly impact the operational costs of hotels. This paper presents an analysis of the annual consumption for the year of 2022 of five hotels located in Crete. An algorithm is also implemented which strives to minimize the capital expenditure (CAPEX), while ensuring a sufficient percentage of self-sufficiency.

Abstract Background In vitro modelling of cancer cells is becoming more complex due to prevailing evidence of intimate interactions between cancer cells and their surrounding stroma. A co-culture system which consists of more than one cell type is physiologically more relevant and thus, could serve as a useful model for various biological studies. An assay that specifically detects the phenotypic changes of cancer cells in a multi-cellular system is lacking for nasopharyngeal carcinoma (NPC). Results Here, we describe a luciferase/luciferin (XenoLuc) assay that could specifically measure changes in the proliferation of cancer cells in the co-culture system using two modified NPC patient-derived tumour xenograft (PDTXs) cells: Xeno284-gfp-luc2 and XenoB110-gfp-luc2. Through this assay, we are able to show that the growth of NPC xenograft cells in both two-dimensional (2D) and three-dimensional (3D) models was enhanced when co-cultured with normal human dermal fibroblasts (NHDFs). In addition, potential applications of this assay in in vitro drug or inhibitor screening experiments are also illustrated. Conclusions XenoLuc assay is specific, sensitive, rapid and cost-effective for measuring the growth of luciferase-expressing cells in a co- or multiple-culture system. This assay may also be adapted for tumour microenvironment studies as well as drug screening experiments in more complex 3D co-culture systems.

The purpose of this research is to describe how to embed entrepreneurial values in early childhood in Kudus. This research was conducted by research method of survey on 36 early childhood educational institution in Kudus in academic year 2017/2018. Data collection techniques are conducted with questionnaire techniques, observations, and interviews. Entrepreneurship education in early childhood educational institution in Kudus aims to foster an entrepreneurial interest and instill social values, economic value, creativity, forming a patient person, appreciating hard-earned, appreciating their own work, disciplined, independent, and responsible. The activity of the planting of entrepreneurial values in early childhood in Kudus is carried out with diverse strategies, ranging from observation to the entrepreneurial places, playing the role of buying and selling, direct buying and selling practices, making something that can be for sale, livestock and farming. Such activities require adequate time, place, and energy. Thus it needs to be planned maturely, so that the implementation of learning runs effectively.

Abstract Control of mosquito vectors by using natural ingredients is being developed intensively. Marigold (Tagetes erecta) was widely used as a mosquito repellent plant. The results of research before had found that marigold leaf extract has a repulsive effect on Aedes aegypti mosquitoes. This study aimed to identify the potential of marigold leaf extract as larvacide against Ae. aegypti larvae in the laboratory. The material used in this research was extracts of marigold leaves. Larvacide test was carried out by dissolving of extract in water at the concentration of 2,000 ppm, 4,000 ppm, 6,000 ppm, 8,000 ppm, and 10,000 ppm. The larvae used was Ae. aegypti larvae as many as 25 larvae each five treatment of replications. Probit analysis was conducted on larval mortality after 24 hours of exposure. The results of the probit analysis showed that the estimated concentration of extracts that could result in larval mortality up to 95% (LC95) was 7,456 ppm. From the result we can conclude that ethanol extract of marigold leaves (T. erecta L. ) has the biolarvacidal activity against larvae. Abstrak Pengendalian vektor nyamuk dengan memanfaatkan bahan alam makin banyak dikembangkan. Marigold (T. erecta) merupakan tumbuhan yang banyak dimanfaatkan sebagai tanaman pengusir nyamuk. Hasil penelitian sebelumnya menemukan bahwa ekstrak daun memiliki daya tolak terhadap nyamuk Ae. aegypti. Penelitian ini bertujuan untuk melihat potensi ekstrak daun marigold sebagai larvasida terhadap larva Ae. aegypti instar III di laboratorium. Bahan yang digunakan pada penelitian ini adalah ekstrak etanol daun marigold hasil ekstraksi dari penelitian sebelumnya. Uji larvasida dilakukan dengan melarutkan ekstrak dalam air pada konsentrasi 2.000 ppm, 4.000 ppm, 6.000 ppm, 8.000 ppm, dan 10.000 ppm. Larva yang digunakan yaitu larva Ae. aegypti sebanyak 25 larva tiap perlakuan dengan lima ulangan. Dilakukan analisis probit terhadap kematian larva setelah 24 jam paparan. Hasil analisa probit menunjukkan perkiraan konsentrasi ekstrak yang mampu mengakibatkan kematian larva hingga 95% (LC95 ) adalah 7.456 ppm. Ekstrak etanol daun marigold (T.erecta L.) memiliki potensi sebagai biolarvasida terhadap larva Ae. aegypti.

Abstract Dengue hemorrhagic fever (DHF) is an infectious arboviral disease transmitted by Aedes mosquitoes. DHF is a public health importance in Indonesia which cause outbreaks in endemic areas. Dengue infection could be fatal if it is not immediately addressed. In January 2016, the dengue outbreak occurred in the Sekarjaya community health center (CHC) working area, Ogan Komering Ulu district leading to one fatal case. A post-outbreak investigation was employed to observe the presence of the Aedes immature stage and to characterize their potential breeding sites. A total of 230 houses were inspected. A high larval density was observed with free-larval index and house index (HI) was 54% and 46%, respectively. Of 876 containers were found; of which 181containers contained larva and 49 containers with Aedes pupae. The container index (CI) and pupal index (PI) was 21% and 6%, respectively. Aedes aegypti positive containers associated with the water source, type,color, the presence of predatory fish, the presence of cover and draining frequency. A routine larval survey needs to be conducted in public places around the residences. Abstrak Demam Berdarah Dengue (DBD) merupakan penyakit menular yang disebabkan oleh virus dengue dan ditularkan oleh nyamuk sebagai vektor. Penyakit ini masih menjadi masalah kesehatan di Indonesia karena sering menimbulkan kejadian luar biasa (KLB) di beberapa daerah endemis. Penyakit ini dapat menimbulkan kematian dalam waktu yang singkat bila tidak segera ditangani. Kejadian luar biasa DBD telah terjadi di wilayah Puskesmas Sekarjaya, Kabupaten Ogan Komering Ulu dengan satu kasus kematian pada bulan Januari 2016. Penelitian ini merupakan studi kasus dengan tujuan untuk mengetahui indeks larva dan karakteristik kontainer yang berpotensi sebagai tempat perkembangbiakan nyamuk . Jumlah rumah yang diperiksa sebanyak 230 Aedes aegypti rumah. Hasil survei jentik mendapatkan angka kepadatan jentik termasuk dalam kepadatan tinggi. Hasil perhitungan indek larva mendapatkan Angka Bebas Jentik (ABJ) sebesar 54% sedangkan sebesar 46%. Jumlah kontainer yang house index (HI) ditemukan sebanyak 876 buah dengan jumlah kontainer positif jentik sebanyak 181 dan kontainer dengan pupa sebanyak 49 buah kontainer. Hasil perhitungan mendapatkan nilai sebesar 21% dan sebesar 6%. container index (CI) pupa index (PI) Karaketeristik yang berhubungan dengan keberadaan jentik kontainer Aedes aegypti adalah sumber air, jenis, warna, pemeliharaan ikan, keberadaan tutup dan pengurasan kontainer. Perlu ditingkatkan kegiatan pemeriksaan jentik di tempat-tempat umum di sekitar pemukiman.

Introduction: One of elderly’s problem in Health Center Karambitan II Tabanan Bali was hypertension. Elderly usually likes or has a habit consuming coffee and salty food everyday. This study was used a pre-experimental one group pre-post test design. The objective of this study was to analyze the effect of consumption limitation of coffee and salt on vital sign in elderly with hypertension. The subjects were elderly with hypertension which stay with their family who lived at Karambitan II Tabanan Bali Health Center region. Method: There were 28 samples which recruited by using total sampling who met to the inclusion criteria. Data were analyzed by using Wilcoxon Sign Rank Test, with significance level α≤0.05. Result: The result showed that there was an effect of consumption limitation of coffee and salt on systolic blood pressure (p=0.00000), diastolic blood pressure (p=0.00000) and heart rate (p=0.00000). Discussion: It can be concluded that there was an effect of consumption limitation of coffee and salt to decrease blood pressure and heart rate in elderly with hypertension who lived at Karambitan II Tabanan Bali Health Center regio. Community nurses should give health education for elderly in public health center periodically for reducing incidence of hypertension.

Duku is a plant that grows in the tropics and has bioactivity as pesticide. Compounds that have the potential as a pesticide on duku is secondary metabolites. This study aimed to determine the correlation between the soil nutrient and secondary metabolites on duku also their larvacidal potency. The research was done by comparing the measurement of physical and chemical soil, phytochemical test and larvacial test against the sample. This study was conducted from February to November 2014, in the Village Simpang Agung South Sumatra province and village Rengas Bandung Jambi Province. The extraction and characterization of secondary metabolites was conducted at the Laboratory of Pharmacy Center, Biomedical and Health Basic Technology, National Institute of Health and Research Development. Larvicidal potency test was conducted in Vector Borne Disease Research and Development Unit Baturaja. The results showed that soil nutrient content of Rengas Bandung Villagewas higher than the Simpang Agung due to the regularly maintaining and fertilizing.The larvicidal test showed extracts from Simpang Agung Village more potent than extracts from the village Rengas Bandung, possibly due to the more secondary metabolites produced in samples from Simpang Agung.

Promotions were given to the Civil Service for services that has reached the work that has been determined by the government, in practice the process of promotion and making SKKPT in proposing the promotion of Civil Servants particularly at the sub-district government Bukit Intan experienced delays in meeting the needs employee documents on the promotion of civil servants. See the existing problems, the research method used for this research is the method of data collection, system development methodology, system approach method of data collection is done by observation and interviews. System development method used is the method Object Oriented. The method of analysis of existing data will be described in terms of activiy Diagram, Use Case. The depiction of the design of the data base will be described in the form Relation Table, Entity-Relationship Diagram (ERD). Promotion of information system design using Visual Basic 2008 program and to the database using Microsoft Access 2000. With the advancement of Information Systems is a data search process more effective and efficient, so as to support the development of Information Systems at the sub-district promotion Bukit Intan Pangkalpinang.

Sangat sulit untuk mendapatkan besarnya kepercayaan atau certainty factor (CF) terhadap gejala yang dialamipenyakit pada anak. Dalam penelitian ini, peneliti mengusulkan suatu metode penghitungan besarnya CF pengguna pada aplikasi sistem pakar untuk evaluasi gangguan pernafasan pada anak dimana penentuan penyakit dengan menggunakan metode certainty factor (CR). Dengan menggunakan metode ini, diharapkan sistem pakar akan menjadi lebih mudah digunakan karena orang tua cukup memasukkan gejala penyakit yang dialami. Sistem secara otomatis akan menghitung nilai CF pengguna. Nilai CF diperoleh dengan menghitung rata-rata gejala yang dimasukkan oleh orang tua terhadap aturan yang diberikan oleh pakar. Metode Certainty factor ini sudah diimplementasikan dalam sistem pakar untuk evalusi gangguan pernafasan pada anak yang dibangun dengan menggunakan bahasa pemrograman Visual Basic 6.0 dan basis data Microsoft Access .  Kata kunci : Sistem Pakar , Certainty Factor , Gangguan Pernafasan pada anak

Administrasi merupakan Pengelolaan data atau arsip terutamadi kelurahan Air Itam yang mana termasuk dalam salah satupelayanan masyarakat. Rekapitulasi arsip perubahan penduduksangat penting. Pelaksanaan pelayanan masyarakat yangsebelumnya masih dilakukan secara manual. DenganPerancangan Sistem pendataan Pendudukini akanmempermudah pengguna di kelurahan Air Itam dalam mengarsipData Kependudukan. Tujuan dari penelitian ini adalah untukmenyediakan Sistem Administrasi terkomputerisasi dalamdatabase. Pengolahan data yang dihasilkan dari sistem akandigunakan untuk pendataan penduduk di Kelurahan Air Itam.Dokumen arsip yang berkaitan dengan tujuan peningkatan mutuAdministrasi kelurahan Air Itam dan meningkatkan pelayananpublik. Hal ini berbeda dengan sistem manual yang telahdigunakan, dengan sistem terkomputerisasi ini risiko kehilangandata dan kerusakan serta untuk menyajikan hasil data lebih rapi.Selain itu, dengan sistem ini, sekertaris akan lebih nyaman danefisien dalam bekerja karena dapat dengan mudah mengaksesinformasi tentang perkembangan masyarakat di daerah tanpakebutuhan untuk menemukan informasi dalam tumpukan kertas. Kata Kunci - DataBase, Konsep UML, Sistem, Sistem informasi,Kependudukan

Torsional deformation and fatigue behaviour of both solid and thin-walled tubular specimens were made from as-received and heat treated 6061 aluminium alloy were studied. 6061 aluminium alloy have been widely used as a candidate material in automobile, aerospace, aircraft and structural application because of their superior mechanical properties such as high strength to weight ratio, good ductility and others. The differences in cyclic deformation and fatigue behaviours between round and solid specimens where a stress gradient exist, and thin-walled tubular specimens where a uniform stress state is commonly assumed, are also discussed. Von Mises and Tresca criteria has been used to predict the monotonic and cyclic deformation curve and compared to the torsional data obtained from the experiment. The S-N curve was used to present and evaluate the fatigue life of the specimens. Through fractographic analysis, failure criteria of fracture surfaces were observed and discussed.

Abstract Background The subtelomeres of many protozoa are highly enriched in genes with roles in niche adaptation. T. cruzi trypomastigotes express surface proteins from Trans-Sialidase (TS) and Dispersed Gene Family-1 (DGF-1) superfamilies which are implicated in host cell invasion. Single populations of T. cruzi may express different antigenic forms of TSs. Analysis of TS genes located at the telomeres suggests that chromosome ends could have been the sites where new TS variants were generated. The aim of this study is to characterize telomeric and subtelomeric regions of T. cruzi available in TriTrypDB and connect the sequences of telomeres to T. cruzi working draft sequence. Results We first identified contigs carrying the telomeric repeat (TTAGGG). Of 49 contigs identified, 45 have telomeric repeats at one end, whereas in four contigs the repeats are located internally. All contigs display a conserved telomeric junction sequence adjacent to the hexamer repeats which represents a signature of T. cruzi chromosome ends. We found that 40 telomeric contigs are located on T. cruzi chromosome-sized scaffolds. In addition, we were able to map several telomeric ends to the chromosomal bands separated by pulsed-field gel electrophoresis. The subtelomeric sequence structure varies widely, mainly as a result of large differences in the relative abundance and organization of genes encoding surface proteins (TS and DGF-1), retrotransposon hot spot genes (RHS), retrotransposon elements, RNA-helicase and N-acetyltransferase genes. While the subtelomeric regions are enriched in pseudogenes, they also contain complete gene sequences matching both known and unknown expressed genes, indicating that these regions do not consist of nonfunctional DNA but are instead functional parts of the expressed genome. The size of the subtelomeric regions varies from 5 to 182 kb; the smaller of these regions could have been generated by a recent chromosome breakage and telomere healing event. Conclusions The lack of synteny in the subtelomeric regions suggests that genes located in these regions are subject to recombination, which increases their variability, even among homologous chromosomes. The presence of typical subtelomeric genes can increase the chance of homologous recombination mechanisms or microhomology-mediated end joining, which may use these regions for the pairing and recombination of free ends.

Abstract The effect of exercise training on the fatty acid composition of erythrocyte membranes was evaluated in an experimental animal model where rats were subjected to a ten-wk aerobic training. Five groups of rats were compared: sedentary rats at 19 or 23 wks of age, rats trained at moderate or high intensity sacrificed at 19 wks of age, and rats trained at high intensity, and sacrificed following 4 weeks of sedentary life. We had already demonstrated that cardioprotection correlates with training intensity and partially persists in detrained rats. Main findings are that rats trained at higher intensity display consistent signs of lipid peroxidation but a lower ω6/ω3 ratio and a lower content of trans fatty acids when compared to rats trained at lower intensity and to older sedentary rats. Trans fatty acids negatively affect cell membrane fluidity and permeability. Detrained rats showed intermediate values. Gene expression evaluation of selected enzymes involved in lipid biosynthesis revealed some of the adaptive mechanisms leading to the maintenance of membrane fatty acid homeostasis following exercise. The decrease in the amount of trans fatty and in the inflammatory pathways (i.e. ω6/ω3 ratio) in high-intensity trained rats underscores the protective effect of high intensity aerobic training.

Abstract Background Different conditions may alter tendon characteristics. Clinical evidence suggests that tendon injuries are more frequent in athletes that change type, intensity and duration of training. Aim of the study was the assessment of training and especially detraining on the patellar tendon (PT) and its enthesis. Methods 27 male adult Sprague-Dawley rats were divided into 3 groups: 20 rats were trained on a treadmill for 10 weeks. Of these, 10 rats were euthanized immediately after training (trained group), and 10 were caged without exercise for 4 weeks before being euthanized (de-trained group). The remaining 7 rats were used as controls (untrained rats). PT insertion, structure (collagen fiber organization and proteoglycan, PG, content), PT thickness, enthesis area, and subchondral bone volume at the enthesis were measured by histomorphometry and microtomography. Results Both PG content and collagen fiber organization were significantly lower in untrained and detrained animals than in trained ones (p < 0.05 and p < 0.0001). In the detrained group, fiber organization and PG content were worse than that of the untrained groups and the untrained group showed a significantly higher score than the detrained group (p < 0.05). In the trained group, the PT was significantly thicker than in untrained group (p < 0.05). No significant differences in the enthesis area and subchondral bone volume among the three groups were seen. Conclusions Moderate exercise exerts a protective effect on the PT structure while sudden discontinuation of physical activity has a negative effect on tendons. The present results suggest that after a period of sudden de-training (such as after an injury) physical activity should be restarted with caution and with appropriate rehabilitation programs.

Abstract Background Paracoccidioides brasiliensis (Eukaryota, Fungi, Ascomycota) is a thermodimorphic fungus, the etiological agent of paracoccidioidomycosis, the most important systemic mycoses in Latin America. Three isolates corresponding to distinct phylogenetic lineages of the Paracoccidioides species complex had their genomes sequenced. In this study the identification and characterization of class II transposable elements in the genomes of these fungi was carried out. Results A genomic survey for DNA transposons in the sequence assemblies of Paracoccidioides, a genus recently proposed to encompass species P. brasiliensis (harboring phylogenetic lineages S1, PS2, PS3) and P. lutzii (Pb01-like isolates), has been completed. Eight new Tc1/mariner families, referred to as Trem (Transposable element mariner), labeled A through H were identified. Elements from each family have 65-80% sequence similarity with other Tc1/mariner elements. They are flanked by 2-bp TA target site duplications and different termini. Encoded DDD-transposases, some of which have complete ORFs, indicated that they could be functionally active. The distribution of Trem elements varied between the genomic sequences characterized as belonging to P. brasiliensis (S1 and PS2) and P. lutzii. TremC and H elements would have been present in a hypothetical ancestor common to P. brasiliensis and P. lutzii, while TremA, B and F elements were either acquired by P. brasiliensis or lost by P. lutzii after speciation. Although TremD and TremE share about 70% similarity, they are specific to P. brasiliensis and P. lutzii, respectively. This suggests that these elements could either have been present in a hypothetical common ancestor and have evolved divergently after the split between P. brasiliensis and P. Lutzii, or have been independently acquired by horizontal transfer. Conclusions New families of Tc1/mariner DNA transposons in the genomic assemblies of the Paracoccidioides species complex are described. Families were distinguished based on significant BLAST identities between transposases and/or TIRs. The expansion of Trem in a putative ancestor common to the species P. brasiliensis and P. lutzii would have given origin to TremC and TremH, while other elements could have been acquired or lost after speciation had occurred. The results may contribute to our understanding of the organization and architecture of genomes in the genus Paracoccidioides.

Abstract Equation with general nonhomogeneous -Laplacian, including classical and singular -Laplacian, is investigated. Necessary and sufficient conditions for the existence of nonoscillatory solutions satisfying certain asymptotic boundary conditions are given and discrepancies between the general and classical are illustrated as well.

Abstract Background Epidemiological studies showed that physical exercise, specifically moderate lifelong training, is protective against cardiovascular morbidity and mortality. Most experimental work has focused into the effects and molecular mechanisms underlying intense, rather than mild exercise, by exploring the acute effect of training. Our study aims at investigating the cardioprotective effect of mild chronic exercise training and the gene expression profile changes at 48 hrs after the exercise cessation. Rats were trained at mild intensity on a treadmill: 25 m/min, 10%incline, 1 h/day, 3 days/week, 10 weeks; about 60% of the maximum aerobic power. By Affymetrix technology, we investigated the gene expression profile induced by exercise training in the left ventricle (LV) of trained (n = 10) and control (n = 10) rats. Cardioprotection was investigated by ischemia/reperfusion experiments (n = 10 trained vs. n = 10 control rats). Results Mild exercise did not induce cardiac hypertrophy and was cardioprotective as demonstrated by the decreased infarct size (p = 0.02) after ischemia/reperfusion experiments in trained with respect to control rats. Ten genes and 2 gene sets (two pathways) resulted altered in LV of exercised animals with respect to controls. We validated by real-time PCR the increased expression of four genes: similar to C11orf17 protein (RGD1306959), caveolin 3, enolase 3, and hypoxia inducible factor 1 alpha. Moreover, caveolin 3 protein levels were higher in exercised than control rats by immunohistochemistry and Western Blot analysis. Interestingly, the predicted gene similar to C11orf17 protein (RGD1306959) was significantly increased by exercise. This gene has a high homology with the human C11orf17 (alias: protein kinase-A interacting protein 1 or breast cancer associated gene 3). This is the first evidence that this gene is involved in the response to the exercise training. Conclusion Our data indicated that few, but significant, genes characterize the gene expression profile of the rat LV, when examined 48 hrs since the last training section and that mild exercise training determines cardioprotection without the induction of hypertrophy.

Abstract Background The aim of our study is to describe a fast molecular method, able to distinguish and quantize the two different genotypes (652 and JP2) of an important periodontal pathogen: Actinobacillus actinomycetemcomitans. The two genotypes show differences in the expression of an important pathogenic factor: the leukotoxin (ltx). In order to evidence this, we performed a real time PCR procedure on the ltx operon, able to recognize Aa clinical isolates with different leukotoxic potentials. Methods The specificity of the method was confirmed in subgingival plaque and saliva specimens collected from eighty-one Italian (Sardinian) subjects with a mean age of 43.9, fifty five (68 %) of whom had various clinical forms of periodontal disease. Results This procedure showed a good sensitivity and a high linear dynamic range of quantization (107-102 cells/ml) for all genotypes and a good correlation factor (R2 = 0.97–0.98). Compared with traditional cultural methods, this real time PCR procedure is more sensitive; in fact in two subgingival plaque and two positive saliva specimens Aa was only detected with the molecular method. Conclusion A low number of Sardinian patients was found positive for Aa infections in the oral cavity, (just 10 positive periodontal cases out of 81 and two of these were also saliva positive). The highly leukotoxic JP2 strain was the most representative (60 % of the positive specimens); the samples from periodontal pockets and from saliva showed some ltx genotype for the same patient. Our experience suggests that this approach is suitable for a rapid and complete laboratory diagnosis for Aa infection.