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15 results on '"Marie-Laure Walet-Balieu"'

1. Comparative Proteomic Analysis of the Diatom Phaeodactylum tricornutum Reveals New Insights Into Intra- and Extra-Cellular Protein Contents of Its Oval, Fusiform, and Triradiate Morphotypes

Author

Coralie Chuberre, Philippe Chan, Marie-Laure Walet-Balieu, François Thiébert, Carole Burel, Julie Hardouin, Bruno Gügi, and Muriel Bardor

Subjects
diatom, Phaeodactylum tricornutum, microalgae, secretome, proteome, morphotype, Plant culture, SB1-1110
Abstract

Phaeodactylum tricornutum is an atypical diatom since it can display three main morphotypes: fusiform, triradiate, and oval. Such pleomorphism is possible thanks to an original metabolism, which is tightly regulated in order to acclimate to environmental conditions. Currently, studies dedicated to the comparison of each morphotype issued from one specific strain are scarce and little information is available regarding the physiological significance of this morphogenesis. In this study, we performed a comparative proteomic analysis of the three morphotypes from P. tricornutum. Cultures highly enriched in one dominant morphotype (fusiform, triradiate, or oval) of P. tricornutum Pt3 strain were used. Pairwise comparisons highlighted biological processes, which are up- and down-regulated in the oval (e.g., purine and cellular amino acid metabolism) and triradiate morphotypes (e.g., oxido-reduction and glycolytic processes) compared to the fusiform one used as a reference. Intersection analysis allowed us to identify the specific features of the oval morphotype. Results from this study confirmed previous transcriptomic RNA sequencing observation showing that the oval cells present a distinct metabolism with specific protein enrichment compared to fusiform and triradiate cells. Finally, the analysis of the secretome of each morphotype was also performed.

Published
2022
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2. IgG N-glycosylation from Patients with Pemphigus Treated with Rituximab

Author

Guillaume Font, Marie-Laure Walet-Balieu, Marie Petit, Carole Burel, Maud Maho-Vaillant, Vivien Hébert, Philippe Chan, Manuel Fréret, Olivier Boyer, Pascal Joly, Sébastien Calbo, Muriel Bardor, and Marie-Laure Golinski

Subjects
pemphigus, rituximab, IgG, glycosylation, N-glycome, N-glycans, Biology (General), QH301-705.5
Abstract

Pemphigus is a life-threatening auto-immune blistering disease of the skin and mucous membrane that is caused by the production of auto-antibodies (auto-Abs) directed against adhesion proteins: desmoglein 1 and 3. We demonstrated in the “Ritux3” trial, the high efficacy of rituximab, an anti-CD20 recombinant monoclonal antibody, as the first-line treatment for pemphigus. However, 25% of patients relapsed during the six-month period after rituximab treatment. These early relapses were associated with a lower decrease in anti-desmoglein auto-Abs after the initial cycle of rituximab. The N-glycosylation of immunoglobulin-G (IgG) can affect their affinity for Fc receptors and their serum half-life. We hypothesized that the extended half-life of Abs could be related to modifications of IgG N-glycans. The IgG N-glycome from pemphigus patients and its evolution under rituximab treatment were analyzed. Pemphigus patients presented a different IgG N-glycome than healthy donors, with less galactosylated, sialylated N-glycans, as well as a lower level of N-glycans bearing an additional N-acetylglucosamine. IgG N-glycome from patients who achieved clinical remission was not different to the one observed at baseline. Moreover, our study did not identify the N-glycans profile as discriminating between relapsing and non-relapsing patients. We report that pemphigus patients present a specific IgG N-glycome. The changes observed in these patients could be a biomarker of autoimmunity susceptibility rather than a sign of inflammation.

Published
2022
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3. N- and O-Glycosylation Pathways in the Microalgae Polyphyletic Group

Author

Elodie Mathieu-Rivet, Narimane Mati-Baouche, Marie-Laure Walet-Balieu, Patrice Lerouge, and Muriel Bardor

Subjects
microalgae, post-translational modification, N-glycosylation, O-glycosylation, protein, biopharmaceuticals, Plant culture, SB1-1110
Abstract

The term microalga refers to various unicellular and photosynthetic organisms representing a polyphyletic group. It gathers numerous species, which can be found in cyanobacteria (i.e., Arthrospira) as well as in distinct eukaryotic groups, such as Chlorophytes (i.e., Chlamydomonas or Chlorella) and Heterokonts (i.e., diatoms). This phylogenetic diversity results in an extraordinary variety of metabolic pathways, offering large possibilities for the production of natural compounds like pigments or lipids that can explain the ever-growing interest of industrials for these organisms since the middle of the last century. More recently, several species have received particular attention as biofactories for the production of recombinant proteins. Indeed, microalgae are easy to grow, safe and cheap making them attractive alternatives as heterologous expression systems. In this last scope of applications, the glycosylation capacity of these organisms must be considered as this post-translational modification of proteins impacts their structural and biological features. Although these mechanisms are well known in various Eukaryotes like mammals, plants or insects, only a few studies have been undertaken for the investigation of the protein glycosylation in microalgae. Recently, significant progresses have been made especially regarding protein N-glycosylation, while O-glycosylation remain poorly known. This review aims at summarizing the recent data in order to assess the state-of-the art knowledge in glycosylation processing in microalgae.

Published
2020
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4. Deciphering the molecular mechanisms of mother-to-egg immune protection in the mealworm beetle Tenebrio molitor.

Author

Guillaume Tetreau, Julien Dhinaut, Richard Galinier, Pascaline Audant-Lacour, Sébastien N Voisin, Karim Arafah, Manon Chogne, Frédérique Hilliou, Anaïs Bordes, Camille Sabarly, Philippe Chan, Marie-Laure Walet-Balieu, David Vaudry, David Duval, Philippe Bulet, Christine Coustau, Yannick Moret, and Benjamin Gourbal

Subjects
Immunologic diseases. Allergy, RC581-607, Biology (General), QH301-705.5
Abstract

In a number of species, individuals exposed to pathogens can mount an immune response and transmit this immunological experience to their offspring, thereby protecting them against persistent threats. Such vertical transfer of immunity, named trans-generational immune priming (TGIP), has been described in both vertebrates and invertebrates. Although increasingly studied during the last decade, the mechanisms underlying TGIP in invertebrates are still elusive, especially those protecting the earliest offspring life stage, i.e. the embryo developing in the egg. In the present study, we combined different proteomic and transcriptomic approaches to determine whether mothers transfer a "signal" (such as fragments of infecting bacteria), mRNA and/or protein/peptide effectors to protect their eggs against two natural bacterial pathogens, namely the Gram-positive Bacillus thuringiensis and the Gram-negative Serratia entomophila. By taking the mealworm beetle Tenebrio molitor as a biological model, our results suggest that eggs are mainly protected by an active direct transfer of a restricted number of immune proteins and of antimicrobial peptides. In contrast, the present data do not support the involvement of mRNA transfer while the transmission of a "signal", if it happens, is marginal and only occurs within 24h after maternal exposure to bacteria. This work exemplifies how combining global approaches helps to disentangle the different scenarios of a complex trait, providing a comprehensive characterization of TGIP mechanisms in T. molitor. It also paves the way for future alike studies focusing on TGIP in a wide range of invertebrates and vertebrates to identify additional candidates that could be specific to TGIP and to investigate whether the TGIP mechanisms found herein are specific or common to all insect species.

Published
2020
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5. Longitudinal Pathogenic Properties and N-Glycosylation Profile of Antibodies from Patients with Pemphigus after Corticosteroid Treatment

Author

Marie Petit, Marie-Laure Walet-Balieu, Damien Schapman, Marie-Laure Golinski, Carole Burel, Marion Barray, Laurent Drouot, Maud Maho-Vaillant, Vivien Hébert, Olivier Boyer, Muriel Bardor, Pascal Joly, and Sébastien Calbo

Subjects
autoantibodies, pemphigus, pathogenic, glycans, Biology (General), QH301-705.5
Abstract

Pemphigus vulgaris is an autoimmune disease that occurs due to pathogenic autoantibodies that recognize the following epidermal adhesion proteins: desmogleins. Systemic corticosteroids usually decrease the titers of anti-desmoglein autoantibodies and improve patients’ conditions. Since modifications of IgG N-glycosylation have been described in some autoimmune diseases, we hypothesized that changes in the pathogenic activity of pemphigus IgG could be related to changes in their N-glycosylation profile. The purpose of this study was to assess, longitudinally, the pathogenicity of pemphigus serum IgG and their N-glycosylation profile during phases of disease activity and clinical remission. The pathogenic activity of serum IgG was measured in vitro on immortalized keratinocytes, by immunofluorescence and dissociation assays, and IgG N-glycans were analyzed by mass spectrometry. We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients’ autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.

Published
2021
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6. Strategies for the Identification of Bioactive Neuropeptides in Vertebrates

Author

Auriane Corbière, Hubert Vaudry, Philippe Chan, Marie-Laure Walet-Balieu, Thierry Lecroq, Arnaud Lefebvre, Charles Pineau, and David Vaudry

Subjects
neuropeptide, identification, peptidomic approach, de novo, bioactiity, review, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571
Abstract

Neuropeptides exert essential functions in animal physiology by controlling e.g., reproduction, development, growth, energy homeostasis, cardiovascular activity and stress response. Thus, identification of neuropeptides has been a very active field of research over the last decades. This review article presents the various methods used to discover novel bioactive peptides in vertebrates. Initially identified on the basis of their biological activity, some neuropeptides have also been discovered for their ability to bind/activate a specific receptor or based on their biochemical characteristics such as C-terminal amidation which concerns half of the known neuropeptides. More recently, sequencing of the genome of many representative species has facilitated peptidomic approaches using mass spectrometry and in silico screening of genomic libraries. Through these different approaches, more than a hundred of bioactive neuropeptides have already been identified in vertebrates. Nevertheless, researchers continue to find new neuropeptides or to identify novel functions of neuropeptides that had not been detected previously, as it was recently the case for nociceptin.

Published
2019
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7. Double impact: natural molluscicide for schistosomiasis vector control also impedes development of Schistosoma mansoni cercariae into adult parasites.

Author

Ronaldo de Carvalho Augusto, Guillaume Tetreau, Philippe Chan, Marie-Laure Walet-Balieu, Clélia Christina Mello-Silva, Claudia Portes Santos, and Christoph Grunau

Subjects
Arctic medicine. Tropical medicine, RC955-962, Public aspects of medicine, RA1-1270
Abstract

Schistosomiasis has been reported in 78 endemic countries and affects 240 million people worldwide. The digenetic parasite Schistosoma mansoni needs fresh water to compete its life cycle. There, it is susceptible to soluble compounds that can affect directly and/or indirectly the parasite's biology. The cercariae stage is one of the key points in which the parasite is vulnerable to different soluble compounds that can significantly alter the parasite's life cycle. Molluscicides are recommended by the World Health Organization for the control of schistosomiasis transmission and Euphorbia milii latex is effective against snails intermediate hosts.We used parasitological tools and electron microscopy to verify the effects of cercariae exposure to natural molluscicide (Euphorbia milii latex) on morphology, physiology and fitness of adult parasite worms. In order to generate insights into key metabolic pathways that lead to the observed phenotypes we used comparative transcriptomics and proteomics.We describe here that the effect of latex on the adult is not due to direct toxicity but it triggers an early change in developmental trajectory and perturbs cell memory, mobility, energy metabolism and other key pathways. We conclude that latex has not only an effect on the vector but applies also long lasting schistosomastatic action. We believe that these results are of interest not only to parasitologists since it shows that natural compounds, presumably without side effects, can have an impact that occurred unexpectedly on developmental processes. Such collateral damage is in this case positive, since it impacts the true target of the treatment campaign. This type of treatment could also provide a rational for the control of other pests. Our results will contribute to enforce the use of E. milii latex in Brazil and other endemic countries as cheap alternative or complement to mass drug treatment with Praziquantel, the only available drug to cure the patients (without preventing re-infection).

Published
2017
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8. IgG

Author

Guillaume, Font, Marie-Laure, Walet-Balieu, Marie, Petit, Carole, Burel, Maud, Maho-Vaillant, Vivien, Hébert, Philippe, Chan, Manuel, Fréret, Olivier, Boyer, Pascal, Joly, Sébastien, Calbo, Muriel, Bardor, and Marie-Laure, Golinski

Abstract

Pemphigus is a life-threatening auto-immune blistering disease of the skin and mucous membrane that is caused by the production of auto-antibodies (auto-Abs) directed against adhesion proteins: desmoglein 1 and 3. We demonstrated in the "Ritux3" trial, the high efficacy of rituximab, an anti-CD20 recombinant monoclonal antibody, as the first-line treatment for pemphigus. However, 25% of patients relapsed during the six-month period after rituximab treatment. These early relapses were associated with a lower decrease in anti-desmoglein auto-Abs after the initial cycle of rituximab. The

Published
2022

9. Comparative Proteomic Analysis of the Diatom

Author

Coralie, Chuberre, Philippe, Chan, Marie-Laure, Walet-Balieu, François, Thiébert, Carole, Burel, Julie, Hardouin, Bruno, Gügi, and Muriel, Bardor

Published
2021

10. Identification of N-glycan oligomannoside isomers in the diatom Phaeodactylum tricornutum

Author

Muriel Bardor, Patrice Lerouge, Marie-Laure Walet-Balieu, Rodolphe Dumontier, Carlos Afonso, Carole Burel, Alain Mareck, Corinne Loutelier-Bourhis, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Chimie Organique et Bioorganique : Réactivité et Analyse (COBRA), Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie Organique Fine (IRCOF), and Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Glycan, Spectrometry, Mass, Electrospray Ionization, Glycans, Glycosylation, Polymers and Plastics, medicine.drug_class, Oligosaccharides, Context (language use), 02 engineering and technology, 010402 general chemistry, Monoclonal antibody, Tandem mass spectrometry, Mass spectrometry, 01 natural sciences, Phaeodactylum tricornutum, law.invention, Isomers, Isomerism, law, Tandem Mass Spectrometry, Ion Mobility Spectrometry, Materials Chemistry, medicine, Microalgae, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], chemistry.chemical_classification, Diatoms, biology, Oligomannosides, Organic Chemistry, Algal Proteins, Diatom, [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics, 021001 nanoscience & nanotechnology, biology.organism_classification, 0104 chemical sciences, [CHIM.POLY]Chemical Sciences/Polymers, chemistry, Biochemistry, biology.protein, Recombinant DNA, 0210 nano-technology, Glycoprotein
Abstract

International audience; Microalgae are emerging production systems for recombinant proteins like monoclonal antibodies. In this context, the characterization of the host cell N-glycosylation machinery and of the microalgae-made biopharmaceuticals, which are mainly glycoprotein-based products, requires efficient analytical methodologies dedicated to the profiling of the N-glycans. Herein, in order to gain knowledge regarding its N-glycosylation pathway, we profile the protein N-linked oligosaccharides isolated from the diatom Phaeodactylum tricornutum that has been used successfully to produce functional monoclonal antibodies. The combination of ion mobility spectrometry–mass Spectrometry and electrospray ionization-multistage tandem mass spectrometry allows us to decipher the detailed structure of the oligomannoside isomers and to demonstrate that the processing of the oligomannosides N-linked to proteins occurs in this diatom as reported in mammals. Therefore, P. tricornutum synthesizes human-like oligomannosides in contrast to other microalgae species. This represent an advantage as an alternative ecofriendly expression system to produce biopharmaceuticals used for human therapy

Published
2021

11. Towards understanding the extensive diversity of protein N‐glycan structures in eukaryotes

Author

Charlotte Toustou, Marie‐Laure Walet‐Balieu, Marie‐Christine Kiefer‐Meyer, Marine Houdou, Patrice Lerouge, François Foulquier, Muriel Bardor, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Unité de Glycobiologie Structurale et Fonctionnelle (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Catholic University of Leuven - Katholieke Universiteit Leuven (KU Leuven), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université de Lille, CNRS, Catholic University of Leuven - Katholieke Universiteit Leuven [KU Leuven], Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF], Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576, and Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale [Glyco-MEV]

Subjects
Life Sciences & Biomedicine - Other Topics, glycosylation, CONGENITAL DISORDER, GLYCOLYLNEURAMINIC ACID, N-glycans, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, eukaryotes, Polysaccharides, glycosyltransferases, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], GLYCOSYLATION PATHWAY, Biology, manganese homeostasis, calcium homeostasis, endoplasmic reticulum, Golgi apparatus, regulation, structural diversity, 030304 developmental biology, CARBOHYDRATE CHAINS, 0303 health sciences, Science & Technology, 030302 biochemistry & molecular biology, Eukaryota, MASS-SPECTROMETRY, GLYCOPROTEINS CONTAIN, ASPARAGINE-LINKED OLIGOSACCHARIDES, carbohydrates (lipids), OLIGOMERIC GOLGI-COMPLEX, CAENORHABDITIS-ELEGANS, General Agricultural and Biological Sciences, Life Sciences & Biomedicine, SCHISTOSOMA-MANSONI
Abstract

N-glycosylation is an important post-translational modification of proteins that has been highly conserved during evolution and is found in Eukaryota, Bacteria and Archaea. In eukaryotes, N-glycan processing is sequential, involving multiple specific steps within the secretory pathway as proteins travel through the endoplasmic reticulum and the Golgi apparatus. In this review, we first summarize the different steps of the N-glycan processing and further describe recent findings regarding the diversity of N-glycan structures in eukaryotic clades. This comparison allows us to explore the different regulation mechanisms of N-glycan processing among eukaryotic clades. Recent findings regarding the regulation of protein N-glycosylation are highlighted, especially the regulation of the biosynthesis of complex-type N-glycans through manganese and calcium homeostasis and the specific role of transmembrane protein 165 (TMEM165) for which homologous sequences have been identified in several eukaryotic clades. Further research will be required to characterize the function of TMEM165 homologous sequences in different eukaryotic clades. ispartof: BIOLOGICAL REVIEWS vol:97 issue:2 pages:732-748 ispartof: location:England status: published

Published
2021
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12. Endogenous Expression of ODN-Related Peptides in Astrocytes Contributes to Cell Protection Against Oxidative Stress: Astrocyte-Neuron Crosstalk Relevance for Neuronal Survival

Author

Pierre Michael Coly, Fatma Amri, Olfa Masmoudi-Kouki, David Vaudry, Jérôme Leprince, Seyma Bahdoudi, Hubert Vaudry, Marie-Laure Walet-Balieu, Ikram Ghouili, Marie-Christine Tonon, Fabrice Morin, Yosra Hamdi, Hélène Castel, Sami Zekri, Mohamed Amri, Université de Tunis El Manar (UTM), Différenciation et communication neuronale et neuroendocrine (DC2N), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Plate-forme de Protéomique PISSARO, Institute for Research and Innovation in Biomedicine (IRIB), Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Faculté de Médecine de Tunis, Plate-Forme de Recherche en Imagerie Cellulaire de Haute-Normandie (PRIMACEN), Normandie Université (NU)-Normandie Université (NU)-Institute for Research and Innovation in Biomedicine (IRIB), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), International Associated laboratory Samuel de Champlain, Institut National de la Recherche Scientifique [Québec] (INRS)-Université de Rouen Normandie (UNIROUEN), Faculté des Sciences Mathématiques, Physiques et Naturelles de Tunis (FST), Department of Biological Sciences, Université de Tunis El Manar (UTM)-Laboratory of Functional Neurophysiology and Pathology, Research Unit UR/11ES09, Faculty of Science of Tunis-Faculty of Science of Tunis, Asymétrie, hétérocycles, hétérochimie et bio-organique (AHHBO), Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), and Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, [SDV]Life Sciences [q-bio], [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], medicine.disease_cause, chemistry.chemical_compound, 0302 clinical medicine, RNA, Small Interfering, Cells, Cultured, ComputingMilieux_MISCELLANEOUS, Diazepam Binding Inhibitor, Neurons, chemistry.chemical_classification, hemic and immune systems, respiratory system, Catalase, Malondialdehyde, Neuroprotection, Cell biology, Neuroprotective Agents, medicine.anatomical_structure, Neurology, Biochemistry, Moderate oxidative stress, Astrocyte, Cell death, Programmed cell death, Cell Survival, Gliopeptide ODN, Neuroscience (miscellaneous), Glio- and neuro-protection, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Biology, 03 medical and health sciences, Cellular and Molecular Neuroscience, medicine, Animals, RNA, Messenger, Rats, Wistar, Reactive oxygen species, Superoxide Dismutase, Neuropeptides, Hydrogen Peroxide, Peptide Fragments, Oxidative Stress, 030104 developmental biology, Metabotropic receptor, chemistry, Apoptosis, Astrocytes, Culture Media, Conditioned, Neuron, 030217 neurology & neurosurgery, Oxidative stress
Abstract

International audience; Astroglial cells are important actors in the defense of brain against oxidative stress injuries. Glial cells synthesize and release the octadecaneuropeptide ODN, a diazepam-binding inhibitor (DBI)-related peptide, which acts through its metabotropic receptor to protect neurons and astrocytes from oxidative stress-induced apoptosis. The purpose of the present study is to examine the contribution of the endogenous ODN in the protection of astrocytes and neurons from moderate oxidative stress. The administration of H2O2 (50 μM, 6 h) induced a moderate oxidative stress in cultured astrocytes, i.e., an increase in reactive oxygen species, malondialdehyde, and carbonyl group levels, but it had no effect on astrocyte death. Mass spectrometry and QPCR analysis revealed that 50 μM H2O2 increased ODN release and DBI mRNA levels. The inhibition of ODN release or pharmacological blockage of the effects of ODN revealed that in these conditions, 50 μM H2O2 induced the death of astrocytes. The transfection of astrocytes with DBI siRNA increased the vulnerability of cells to moderate stress. Finally, the addition of 1 nM ODN to culture media reversed cell death observed in DBI-deficient astrocytes. The treatment of neurons with media from 50 μM H2O2-stressed astrocytes significantly reduced the neuronal death induced by H2O2; this effect is greatly attenuated by the administration of an ODN metabotropic receptor antagonist. Overall, these results indicate that astrocytes produce authentic ODN, notably in a moderate oxidative stress situation, and this glio- and neuro-protective agent may form part of the brain defense mechanisms against oxidative stress injury.

Published
2018
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13. A Peptidomic Approach to Characterize Peptides Involved in Cerebellar Cortex Development Leads to the Identification of the Neurotrophic Effects of Nociceptin

Author

Marie-Laure Walet-Balieu, Philippe Chan, Auriane Corbière, David Vaudry, Magali Basille-Dugay, Julie Hardouin, Lebon, Alexis, Différenciation et communication neuronale et neuroendocrine (DC2N), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Plate-forme de Protéomique PISSARO, Institute for Research and Innovation in Biomedicine (IRIB), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-High-tech Research Infrastructures for Life Sciences (HeRacLeS), Normandie Université (NU)-Normandie Université (NU)-Institute for Research and Innovation in Biomedicine (IRIB), Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Plate-Forme de Recherche en Imagerie Cellulaire de Haute-Normandie (PRIMACEN), Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-High-tech Research Infrastructures for Life Sciences (HeRacLeS), and Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, Male, Proteomics, MESH: Receptors, Opioid, Cerebellum, [SDV]Life Sciences [q-bio], MESH: Neurons, Apoptosis, MESH: Amino Acid Sequence, Biochemistry, Nociceptin Receptor, Analytical Chemistry, 0302 clinical medicine, MESH: Opioid Peptides, MESH: Animals, Receptor, Cells, Cultured, Neurons, biology, MESH: Peptides, MESH: Proteomics, Cell Differentiation, [SDV] Life Sciences [q-bio], Nociceptin receptor, medicine.anatomical_structure, Somatostatin, Opioid Peptides, Cerebellar cortex, MESH: Hydrogen Peroxide, Female, Neurotrophin, MESH: Cells, Cultured, MESH: Cell Differentiation, Neuropeptide, 03 medical and health sciences, Cerebellar Cortex, medicine, Animals, Amino Acid Sequence, Nerve Growth Factors, RNA, Messenger, Rats, Wistar, Molecular Biology, MESH: RNA, Messenger, MESH: Cerebellar Cortex, MESH: Nerve Growth Factors, Research, MESH: Apoptosis, Hydrogen Peroxide, MESH: Rats, Wistar, Granule cell, MESH: Cerebellum, MESH: Male, 030104 developmental biology, nervous system, Receptors, Opioid, biology.protein, Peptides, Neuroscience, MESH: Female, 030217 neurology & neurosurgery
Abstract

International audience; The cerebellum is a brain structure involved in motor and cognitive functions. The development of the cerebellar cortex (the external part of the cerebellum) is under the control of numerous factors. Among these factors, neuropeptides including PACAP or somatostatin modulate the survival, migration and/or differentiation of cerebellar granule cells. Interestingly, such peptides contributing to cerebellar ontogenesis usually exhibit a specific transient expression profile with a low abundance at birth, a high expression level during the developmental processes, which take place within the first two postnatal weeks in rodents, and a gradual decline toward adulthood. Thus, to identify new peptides transiently expressed in the cerebellum during development, rat cerebella were sampled from birth to adulthood, and analyzed by a semi-quantitative peptidomic approach. A total of 33 peptides were found to be expressed in the cerebellum. Among these 33 peptides, 8 had a clear differential expression pattern during development, 4 of them i.e. cerebellin 2, nociceptin, somatostatin and VGF [353-372], exhibiting a high expression level during the first two postnatal weeks followed by a significative decrease at adulthood. A focus by a genomic approach on nociceptin, confirmed that its precursor mRNA is transiently expressed during the first week of life in granule neurons within the internal granule cell layer of the cerebellum, and showed that the nociceptin receptor is also actively expressed between P8 and P16 by the same neurons. Finally, functional studies revealed a new role for nociceptin, acting as a neurotrophic peptide able to promote the survival and differentiation of developing cerebellar granule neurons.

Published
2018
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14. Double impact: natural molluscicide for schistosomiasis vector control also impedes development of Schistosoma mansoni cercariae into adult parasites

Author

Christoph Grunau, Guillaume Tetreau, Marie-Laure Walet-Balieu, Philippe Chan, Ronaldo de Carvalho Augusto, Clélia Christina Mello-Silva, Cláudia Portes Santos, Fundação Oswaldo Cruz (FIOCRUZ), Réseau International des Instituts Pasteur (RIIP), Interactions Hôtes-Pathogènes-Environnements (IHPE), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Perpignan Via Domitia (UPVD), Plate-forme de Protéomique PISSARO, Institute for Research and Innovation in Biomedicine (IRIB), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), and This study received funding from Wellcome Trust (Flatworm Functional Genomics Initiative (FUGI) - 107475/Z/15/Z), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES Parasitologia Básica/2011), Fundação Carlos Chagas Filho de Amparo à Pesquisa no Rio de Janeiro (FAPERJ–BIOTA 2012), Conselho Nacional de Pesquisa (CNPq) – Edital Universal 2014 and PAEF/Fiocruz (PAEF no. IOC-008-FIO-04), CNPq fellowships (205872/2014-0 – SWE) and MATER-IMMUNITY Project (ANR-14-CE02-0009) of the French National Research Agency (ANR).

Subjects
Male, 0301 basic medicine, Schistosoma Mansoni, Latex, Euphorbia milii, Gene Expression, Biomphalaria, Pathogenesis, MESH: Latex/administration & dosage, Pathology and Laboratory Medicine, Biochemistry, Parasite load, Parasite Load, MESH: Linear Models, Mice, 0302 clinical medicine, Animal Cells, MESH: Sequence Analysis, RNA, Medicine and Health Sciences, Schistosomiasis, Parasite hosting, MESH: Animals, MESH: Cercaria/growth & development, Cercaria, MESH: Molluscacides/administration & dosage, biology, lcsh:Public aspects of medicine, 3. Good health, MESH: Schistosoma mansoni/drug effects, Praziquantel, Infectious Diseases, Liver, Helminth Infections, Molluscicide, Physical Sciences, Host-Pathogen Interactions, Granulomas, Schistosoma, Emulsions, Female, Metabolic Pathways, Schistosoma mansoni, MESH: Cercaria/drug effects, Cellular Types, Brazil, Research Article, Neglected Tropical Diseases, medicine.drug, lcsh:Arctic medicine. Tropical medicine, Molluscacides, lcsh:RC955-962, Materials by Structure, Immune Cells, Materials Science, Immunology, 030231 tropical medicine, MESH: Microscopy, Electron, MESH: Liver/pathology, MESH: Schistosoma mansoni/ultrastructure, 03 medical and health sciences, Helminths, parasitic diseases, Parasitic Diseases, Genetics, medicine, Animals, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, Colloids, MESH: Mice, MESH: Biomphalaria/parasitology, Plant Extracts, Sequence Analysis, RNA, Organisms, Public Health, Environmental and Occupational Health, Biology and Life Sciences, lcsh:RA1-1270, Cell Biology, MESH: Schistosomiasis mansoni/parasitology, Tropical Diseases, biology.organism_classification, medicine.disease, Invertebrates, MESH: Male, Schistosomiasis mansoni, MESH: Parasite Load, Microscopy, Electron, Metabolism, 030104 developmental biology, Mixtures, Linear Models, MESH: Schistosomiasis mansoni/prevention & control, MESH: Plant Extracts/administration & dosage, MESH: Brazil, MESH: Female
Abstract

Background Schistosomiasis has been reported in 78 endemic countries and affects 240 million people worldwide. The digenetic parasite Schistosoma mansoni needs fresh water to compete its life cycle. There, it is susceptible to soluble compounds that can affect directly and/or indirectly the parasite’s biology. The cercariae stage is one of the key points in which the parasite is vulnerable to different soluble compounds that can significantly alter the parasite’s life cycle. Molluscicides are recommended by the World Health Organization for the control of schistosomiasis transmission and Euphorbia milii latex is effective against snails intermediate hosts. Methodology/Principal findings We used parasitological tools and electron microscopy to verify the effects of cercariae exposure to natural molluscicide (Euphorbia milii latex) on morphology, physiology and fitness of adult parasite worms. In order to generate insights into key metabolic pathways that lead to the observed phenotypes we used comparative transcriptomics and proteomics. Conclusions/Significance We describe here that the effect of latex on the adult is not due to direct toxicity but it triggers an early change in developmental trajectory and perturbs cell memory, mobility, energy metabolism and other key pathways. We conclude that latex has not only an effect on the vector but applies also long lasting schistosomastatic action. We believe that these results are of interest not only to parasitologists since it shows that natural compounds, presumably without side effects, can have an impact that occurred unexpectedly on developmental processes. Such collateral damage is in this case positive, since it impacts the true target of the treatment campaign. This type of treatment could also provide a rational for the control of other pests. Our results will contribute to enforce the use of E. milii latex in Brazil and other endemic countries as cheap alternative or complement to mass drug treatment with Praziquantel, the only available drug to cure the patients (without preventing re-infection)., Author summary Intestinal schistosomiasis is among the most important parasitic disease caused by helminthes, affecting 67 million people worldwide. Vector and intermediate host of the parasitic worm are fresh water snails. WHO recommends use of molluscicides for control of local transmission. Among those, natural plant extracts such as Euphorbia milii latex have attracted particular attention since they are sustainable and cheap. We had anecdotic evidence that E. milii latex also impacts infection outcome if treated snails were infected with S. mansoni. We show here that transient exposure of the human dwelling larvae (cercariae) to the latex at doses that do not affect its infectivity has effects 60 days later on the morphology, physiology and fitness of the adult parasite worms. In order to generate insights into key metabolic pathways that lead to the observed phenotypes we used comparative transcriptomics and proteomics. We show that the effect of latex on the adult is not due to direct toxicity but it triggers an early change in developmental trajectory and perturbs cell memory, mobility, energy metabolism and other key pathways. We conclude that latex has not only an effect on the vector but applies also long lasting schistosomastatic action. The present work might also provide insights on targets with implications for developing new interventions for schistosomiasis control.

Published
2017
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15. Heterologous expression of the N-acetylglucosaminyltransferase I dictates a reinvestigation of the N-glycosylation pathway in Chlamydomonas reinhardtii

Author

Carole Plasson, Vincent Richard, Corinne Loutelier-Bourhis, Azeddine Driouich, Marie-Laure Walet-Balieu, Philippe Chan Tchi-Song, Sophie Bernard, Gaëtan Vanier, Carlos Afonso, Elodie Mathieu-Rivet, Muriel Bardor, Isabelle Remy-Jouet, Patrice Lerouge, Jessica Vanier, Pierre-Louis Lucas, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Chimie Organique et Bioorganique : Réactivité et Analyse (COBRA), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie Organique Fine (IRCOF), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Plate-forme de Protéomique PISSARO, Institute for Research and Innovation in Biomedicine (IRIB), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Endothélium, valvulopathies et insuffisance cardiaque (EnVI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rouen Normandie (UNIROUEN), Institut de Recherche Interdisciplinaire en Sciences Sociales (IRISSO), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Paris Dauphine-PSL, Vanier, Gaëtan, Lucas , Pierre-Louis, Bardor, Muriel, Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie Organique Fine (IRCOF), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université Paris Dauphine-PSL, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), University of Rouen Normandie, Region Normandie, Institut Universitaire de France (I.U.F.), European regional development fund (ERDF) [31708], Labex SynOrg [ANR-11-LABX-0029], Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rouen Normandie (UNIROUEN), and Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
0106 biological sciences, 0301 basic medicine, Glycan, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Glycosylation, Chlamydomonas reinhardtii, lcsh:Medicine, Vacuole, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], N-Acetylglucosaminyltransferases, 01 natural sciences, Article, 03 medical and health sciences, symbols.namesake, N-linked glycosylation, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Glycosyltransferase, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], lcsh:Science, ComputingMilieux_MISCELLANEOUS, [SDV.BDD.GAM]Life Sciences [q-bio]/Development Biology/Gametogenesis, Plant Proteins, Diatoms, Multidisciplinary, biology, lcsh:R, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], Golgi apparatus, biology.organism_classification, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], Complementation, carbohydrates (lipids), [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [CHIM.POLY]Chemical Sciences/Polymers, 030104 developmental biology, Biochemistry, biology.protein, symbols, lcsh:Q, Heterologous expression, 010606 plant biology & botany
Abstract

Eukaryotic N-glycosylation pathways are dependent of N-acetylglucosaminyltransferase I (GnTI), a key glycosyltransferase opening the door to the formation of complex-type N-glycans by transferring a N-acetylglucosamine residue onto the Man5GlcNAc2 intermediate. In contrast, glycans N-linked to Chlamydomonas reinhardtii proteins arise from a GnTI-independent Golgi processing of oligomannosides giving rise to Man5GlcNAc2 substituted eventually with one or two xylose(s). Here, complementation of C. reinhardtii with heterologous GnTI was investigated by expression of GnTI cDNAs originated from Arabidopsis and the diatom Phaeodactylum tricornutum. No modification of the N-glycans was observed in the GnTI transformed cells. Consequently, the structure of the Man5GlcNAc2 synthesized by C. reinhardtii was reinvestigated. Mass spectrometry analyses combined with enzyme sequencing showed that C. reinhardtii proteins carry linear Man5GlcNAc2 instead of the branched structure usually found in eukaryotes. Moreover, characterization of the lipid-linked oligosaccharide precursor demonstrated that C. reinhardtii exhibit a Glc3Man5GlcNAc2 dolichol pyrophosphate precursor. We propose that this precursor is then trimmed into a linear Man5GlcNAc2 that is not substrate for GnTI. Furthermore, cells expressing GnTI exhibited an altered phenotype with large vacuoles, increase of ROS production and accumulation of starch granules, suggesting the activation of stress responses likely due to the perturbation of the Golgi apparatus.

Published
2017
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