1. Ezetimibe increases intestinal expression of the LDL receptor gene in dyslipidaemic men with insulin resistance
- Author
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Jean-Philippe Drouin-Chartier, Benoît Lamarche, Marie-Claude Lépine, Patrick Couture, André J. Tremblay, and Valéry Lemelin
- Subjects
0301 basic medicine ,medicine.medical_specialty ,business.industry ,Endocrinology, Diabetes and Metabolism ,PCSK9 ,030204 cardiovascular system & hematology ,medicine.disease ,Small intestine ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Endocrinology ,Real-time polymerase chain reaction ,Insulin resistance ,medicine.anatomical_structure ,Ezetimibe ,Internal medicine ,Gene expression ,LDL receptor ,Internal Medicine ,Intestinal cholesterol absorption ,medicine ,business ,medicine.drug - Abstract
Aim To gain further insight into intestinal cholesterol homeostasis in dyslipidaemic men with insulin resistance (IR) by examining the impact of treatment with ezetimibe on the expression of key genes involved in cholesterol synthesis and LDL receptor (R)-mediated uptake of lipoproteins. Methods A total of 25 men with dyslipidaemia and IR were recruited to participate in this double-blind, randomized, crossover, placebo-controlled trial. Participants received 10 mg/day ezetimibe or placebo for periods of 12 weeks each. Intestinal gene expression was measured by quantitative PCR in duodenal biopsy samples collected by gastroduodenoscopy at the end of each treatment. Results A total of 20 participants completed the protocol. Treatment with ezetimibe significantly increased intestinal LDLR (+16.2%; P = .01), 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMG-CoAR; +14.0%; P = .04) and acetyl-Coenzyme A acetyltransferase 2 (ACAT-2) mRNA expression (+12.5%; P = .03). Changes in sterol regulatory element-binding transcription factor 2 (SREBP-2) expression were significantly correlated with changes in HMG-CoAR (r = 0.55; P
- Published
- 2016