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1. Perinatal derivatives application: Identifying possibilities for clinical use

Author

Florelle Gindraux, Nicola Hofmann, Marta Agudo-Barriuso, Mariastefania Antica, Pedro Silva Couto, Marie Dubus, Serhiy Forostyak, Lenart Girandon, Roberto Gramignoli, Marcin Jurga, Sergio Liarte, Ruta Navakauskiene, Volodymyr Shablii, Xavier Lafarge, and Francisco J. Nicolás

Subjects
clinical trials, perinatal derivatives, ICD-10 = international classification of diseases, questionnaire for PnD use in human conditions, amniotic membrane, Biotechnology, TP248.13-248.65
Abstract

Perinatal derivatives are drawing growing interest among the scientific community as an unrestricted source of multipotent stromal cells, stem cells, cellular soluble mediators, and biological matrices. They are useful for the treatment of diseases that currently have limited or no effective therapeutic options by means of developing regenerative approaches. In this paper, to generate a complete view of the state of the art, a comprehensive 10-years compilation of clinical-trial data with the common denominator of PnD usage has been discussed, including commercialized products. A set of criteria was delineated to challenge the 10-years compilation of clinical trials data. We focused our attention on several aspects including, but not limited to, treated disorders, minimal or substantial manipulation, route of administration, dosage, and frequency of application. Interestingly, a clear correlation of PnD products was observed within conditions, way of administration or dosage, suggesting there is a consolidated clinical practice approach for the use of PnD in medicine. No regulatory aspects could be read from the database since this information is not mandatory for registration. The database will be publicly available for consultation. In summary, the main aims of this position paper are to show possibilities for clinical application of PnD and propose an approach for clinical trial preparation and registration in a uniform and standardized way. For this purpose, a questionnaire was created compiling different sections that are relevant when starting a new clinical trial using PnD. More importantly, we want to bring the attention of the medical community to the perinatal products as a consolidated and efficient alternative for their use as a new standard of care in the clinical practice.

Published
2022
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2. Methods and criteria for validating the multimodal functions of perinatal derivatives when used in oncological and antimicrobial applications

Author

Antonietta R. Silini, Taja Železnik Ramuta, Ana Salomé Pires, Asmita Banerjee, Marie Dubus, Florelle Gindraux, Halima Kerdjoudj, Justinas Maciulatis, Adelheid Weidinger, Susanne Wolbank, Günther Eissner, Bernd Giebel, Michela Pozzobon, Ornella Parolini, and Mateja Erdani Kreft

Subjects
perinatal derivatives, biological assays, functional assays, potency assays, mechanisms of action, pharmacologic actions, Biotechnology, TP248.13-248.65
Abstract

Perinatal derivatives or PnDs refer to tissues, cells and secretomes from perinatal, or birth-associated tissues. In the past 2 decades PnDs have been highly investigated for their multimodal mechanisms of action that have been exploited in various disease settings, including in different cancers and infections. Indeed, there is growing evidence that PnDs possess anticancer and antimicrobial activities, but an urgent issue that needs to be addressed is the reproducible evaluation of efficacy, both in vitro and in vivo. Herein we present the most commonly used functional assays for the assessment of antitumor and antimicrobial properties of PnDs, and we discuss their advantages and disadvantages in assessing the functionality. This review is part of a quadrinomial series on functional assays for the validation of PnDs spanning biological functions such as immunomodulation, anticancer and antimicrobial, wound healing, and regeneration.

Published
2022
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3. Staphylococcus aureus Behavior on Artificial Surfaces Mimicking Bone Environment

Author

Anaïs Lemaire, Jennifer Varin-Simon, Fabien Lamret, Marie Dubus, Halima Kerdjoudj, Frédéric Velard, Sophie C. Gangloff, and Fany Reffuveille

Subjects
Staphylococcus aureus, adhesion, biofilm, bone substitutes, Medicine
Abstract

Infections, which interfere with bone regeneration, may be a critical issue to consider during the development of biomimetic material. Calcium phosphate (CaP) and type I collagen substrates, both suitable for bone-regeneration dedicated scaffolds, may favor bacterial adhesion. Staphylococcus aureus possesses adhesins that allow binding to CaP or collagen. After their adhesion, bacteria may develop structures highly tolerant to immune system attacks or antibiotic treatments: the biofilms. Thus, the choice of material used for scaffolds intended for bone sites is essential to provide devices with the ability to prevent bone and joint infections by limiting bacterial adhesion. In this study, we compared the adhesion of three different S. aureus strains (CIP 53.154, SH1000, and USA300) on collagen- and CaP-coating. Our objective was to evaluate the capacity of bacteria to adhere to these different bone-mimicking coated supports to better control the risk of infection. The three strains were able to adhere to CaP and collagen. The visible matrix components were more important on CaP- than on collagen-coating. However, this difference was not reflected in biofilm gene expression for which no change was observed between the two tested surfaces. Another objective was to evaluate these bone-mimicking coatings for the development of an in vitro model. Thus, CaP, collagen-coatings, and the titanium-mimicking prosthesis were simultaneously tested in the same bacterial culture. No significant differences were found compared to adhesion on surfaces independently tested. In conclusion, these coatings used as bone substitutes can easily be colonized by bacteria, especially CaP-coating, and must be used with an addition of antimicrobial molecules or strategies to avoid bacterial biofilm development.

Published
2023
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4. Hybrid Mineral/Organic Material Induces Bone Bridging and Bone Volume Augmentation in Rat Calvarial Critical Size Defects

Author

Marie Dubus, Loïc Scomazzon, Charlotte Ledouble, Julien Braux, Abdelilah Beljebbar, Laurence Van Gulick, Adrien Baldit, Caroline Gorin, Halima Alem, Nicole Bouland, Marissa Britton, Jessica Schiavi, Ted J. Vaughan, Cédric Mauprivez, and Halima Kerdjoudj

Subjects
dicalcium phosphate dihydrate, polysaccharides, hybrid bioactive materials, critical-sized bone defect, bone volume augmentation, multi-scale characterization, Cytology, QH573-671
Abstract

In craniofacial bone defects, the promotion of bone volume augmentation remains a challenge. Finding strategies for bone regeneration such as combining resorbable minerals with organic polymers would contribute to solving the bone volume roadblock. Here, dicalcium phosphate dihydrate, chitosan and hyaluronic acid were used to functionalize a bone-side collagen membrane. Despite an increase in the release of inflammatory mediators by human circulating monocytes, the in vivo implantation of the functionalized membrane allowed the repair of a critical-sized defect in a calvaria rat model with de novo bone exhibiting physiological matrix composition and structural organization. Microtomography, histological and Raman analysis combined with nanoindentation testing revealed an increase in bone volume in the presence of the functionalized membrane and the formation of woven bone after eight weeks of implantation; these data showed the potential of dicalcium phosphate dihydrate, chitosan and hyaluronic acid to induce an efficient repair of critical-sized bone defects and establish the importance of thorough multi-scale characterization in assessing biomaterial outcomes in animal models.

Published
2022
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5. Infection of Human Dental Pulp Stromal Cells by Streptococcus mutans: Shedding Light on Bacteria Pathogenicity and Pulp Inflammation

Author

Elodie Maisonneuve, Julie Chevrier, Marie Dubus, Jennifer Varin, Johan Sergheraert, Sophie C. Gangloff, Fany Reffuveille, Cédric Mauprivez, and Halima Kerdjoudj

Subjects
dental pulp derived stromal cells, inflammation, internalization, S. mutans, pathogenicity, Biology (General), QH301-705.5
Abstract

Cariogenic Streptococcus mutans (S. mutans) is implicated in the dental pulp necrosis but also in cardiovascular tissue infections. Herein, the purpose was to elucidate how human dental pulp derived stromal cells (DPSCs) react toward a direct interaction with S. mutans. DPSCs were challenged with S. mutans. Following 3 h of interaction, DPSCs were able to internalize S. mutans (rate < 1%), and F-actin fibers played a significant role in this process. S. mutans persisted in the DPSCs for 48 h without causing a cytotoxic effect. S. mutans was, however, able to get out of the DPSCs cytoplasm and to proliferate in the extracellular environment. Yet, we noticed several adaptive responses of bacteria to the extracellular environment such as a modification of the kinetic growth, the increase in biofilm formation on type I collagen and polyester fabrics, as well as a tolerance toward amoxicillin. In response to infection, DPSCs adopted a proinflammatory profile by increasing the secretion of IL-8, lL-1β, and TNF-α, strengthening the establishment of the dental pulp inflammation. Overall, these findings showed a direct impact of S. mutans on DPSCs, providing new insights into the potential role of S. mutans in infective diseases.

Published
2020
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6. Antibacterial and Immunomodulatory Properties of Acellular Wharton’s Jelly Matrix

Author

Marie Dubus, Loïc Scomazzon, Julie Chevrier, Charlotte Ledouble, Adrien Baldit, Julien Braux, Florelle Gindraux, Camille Boulagnon, Sandra Audonnet, Marius Colin, Hassan Rammal, Cédric Mauprivez, and Halima Kerdjoudj

Subjects
Wharton’s jelly, decellularization, bioactivity, antibacterial, immunomodulation, Biology (General), QH301-705.5
Abstract

Of all biologic matrices, decellularized tissues have emerged as a promising tool in the field of regenerative medicine. Few empirical clinical studies have shown that Wharton’s jelly (WJ) of the human umbilical cord promotes wound closure and reduces wound-related infections. In this scope, we herein investigated whether decellularized (DC)-WJ could be used as an engineered biomaterial. In comparison with devitalized (DV)-WJ, our results showed an inherent effect of DC-WJ on Gram positive (S. aureus and S. epidermidis) and Gram negative (E. coli and P. aeruginosa) growth and adhesion. Although DC-WJ activated the neutrophils and monocytes in a comparable magnitude to DV-WJ, macrophages modulated their phenotypes and polarization states from the resting M0 phenotype to the hybrid M1/M2 phenotype in the presence of DC-WJ. M1 phenotype was predominant in the presence of DV-WJ. Finally, the subcutaneous implantation of DC-WJ showed total resorption after three weeks of implantation without any sign of foreign body reaction. These significant data shed light on the potential regenerative application of DC-WJ in providing a suitable biomaterial for tissue regenerative medicine and an ideal strategy to prevent wound-associated infections.

Published
2022
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7. Osteoinductive Material to Fine-Tune Paracrine Crosstalk of Mesenchymal Stem Cells With Endothelial Cells and Osteoblasts

Author

Hassan Rammal, Laura Entz, Marie Dubus, Aurélie Moniot, Nicolae B. Bercu, Johan Sergheraert, Sophie C. Gangloff, Cédric Mauprivez, and Halima Kerdjoudj

Subjects
mesenchymal stem cells, paracrine activities, cell crosstalk, osteoinductive material, culture media, Biotechnology, TP248.13-248.65
Abstract

While stem cell/biomaterial studies provide solid evidences that biomaterial intrinsic cues deeply affect cell fate, current strategies tend to neglect their effects on mesenchymal stem cells (MSCs) secretory activities and resulting cell-crosstalks. The present study aims to investigate the impact of bone-mimetic material (B-MM), with intrinsic osteoinductive property, on MSCs mediator secretions; and to explore underlying effects on cells involved in bone regeneration. Human MSCs were cultured, on B-MM, made from inorganic calcium phosphate supplemented with chitosan and hyaluronic acid biopolymers. Collected MSCs culture media were assessed for mediators release quantification and used further to stimulate endothelial cells (ECs) and alveolar bone derived osteoblasts (OBs). Without osteogenic supplements, MSCs committed into bone lineage forming thus 3D bone-like nodules after 21 days. Despite a weak percentage of cell commitment, our data elucidate new aspects of osteoinductive material effect on MSCs functions through the regulation of the secretion of mediators involved in bone regeneration and subsequently the MSCs/ECs indirect crosstalk with osteogenesis-boosting effect. Using MSCs culture media, we demonstrate a large potential of osteoinductive materials and MSCs in bone regenerative medicine. Such strategies could help to address some insights in cell-free therapies using MSCs derived media.

Published
2019
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8. Bone Environment Influences Irreversible Adhesion of a Methicillin-Susceptible Staphylococcus aureus Strain

Author

Fany Reffuveille, Jérôme Josse, Frédéric Velard, Fabien Lamret, Jennifer Varin-Simon, Marie Dubus, Evan F. Haney, Robert E. W. Hancock, Céline Mongaret, and Sophie C. Gangloff

Subjects
bone and joint infections, biofilm, antibiofilm peptides, bone environment, bacterial starvation, Microbiology, QR1-502
Abstract

Prosthesis and joint infections are an important threat in public health, especially due to the development of bacterial biofilms and their high resistance to antimicrobials. Biofilm-associated infections increase mortality and morbidity rates as well as hospitalization costs. Prevention is the best strategy for this serious issue, so there is an urgent need to understand the signals that could induce irreversible bacterial adhesion on a prosthesis. In this context, we investigated the influence of the bone environment on surface adhesion by a methicillin-susceptible Staphylococcus aureus strain. Using static and dynamic biofilm models, we tested various bone environment factors and showed that the presence of Mg2+, lack of oxygen, and starvation each increased bacterial adhesion. It was observed that human osteoblast-like cell culture supernatants, which contain secreted components that would be found in the bone environment, increased bacterial adhesion capacity by 2-fold (p = 0.015) compared to the medium control. Moreover, supernatants from osteoblast-like cells stimulated with TNF-α to mimic inflammatory conditions increased bacterial adhesion by almost 5-fold (p = 0.003) without impacting on the overall biomass. Interestingly, the effect of osteoblast-like cell supernatants on bacterial adhesion could be counteracted by the activity of synthetic antibiofilm peptides. Overall, the results of this study demonstrate that factors within the bone environment and products of osteoblast-like cells directly influence S. aureus adhesion and could contribute to biofilm initiation on bone and/or prosthetics implants.

Published
2018
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9. Pellet-Based Fused Filament Fabrication (FFF)-Derived Process for the Development of Polylactic Acid/Hydroxyapatite Scaffolds Dedicated to Bone Regeneration

Author

Marie Bayart, Marie Dubus, Sébastien Charlon, Halima Kerdjoudj, Nicolas Baleine, Samira Benali, Jean-Marie Raquez, Jérémie Soulestin, Centre for Materials and Processes (CERI MP - IMT Nord Europe), Ecole nationale supérieure Mines-Télécom Lille Douai (IMT Nord Europe), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Centre d'Innovation et de Recherche en Matériaux Polymères (CIRMAP), Université de Mons (UMons), and Colin, Marius

Subjects
[SDV.IB.BIO] Life Sciences [q-bio]/Bioengineering/Biomaterials, bone regeneration, scaffolds, fused filament fabrication (FFF), polylactic acid (PLA), hydroxyapatite, General Materials Science, [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials, Bone regeneration
Abstract

International audience; Scaffolds can be defined as 3D architectures with specific features (surface properties, porosity, rigidity, biodegradability, etc.) that help cells to attach, proliferate, and to differentiate into specific lineage. For bone regeneration, rather high mechanical properties are required. That is why polylactic acid (PLA) and PLA/hydroxyapatite (HA) scaffolds (10 wt.%) were produced by a peculiar fused filament fabrication (FFF)-derived process. The effect of the addition of HA particles in the scaffolds was investigated in terms of morphology, biological properties, and biodegradation behavior. It was found that the scaffolds were biocompatible and that cells managed to attach and proliferate. Biodegradability was assessed over a 5-month period (according to the ISO 13781-Biodegradability norm) through gel permeation chromatography (GPC), differential scanning calorimetry (DSC), and compression tests. The results revealed that the presence of HA in the scaffolds induced a faster and more complete polymer biodegradation, with a gradual decrease in the molar mass (Mn) and compressive mechanical properties over time. In contrast, the Mn of PLA only decreased during the processing steps to obtain scaffolds (extrusion + 3D-printing) but PLA scaffolds did not degrade during conditioning, which was highlighted by a high retention of the mechanical properties of the scaffolds after conditioning.

Published
2022
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10. Biomechanical tensile behavior of human Wharton’s jelly

Author

Johan Sergheraert, Adrien Baldit, Cédric Mauprivez, Marie Dubus, Halima Kerdjoudj, Rachid Rahouadj, Laboratoire d'Etude des Microstructures et de Mécanique des Matériaux (LEM3), Centre National de la Recherche Scientifique (CNRS)-Université de Lorraine (UL)-Arts et Métiers Sciences et Technologies, HESAM Université (HESAM)-HESAM Université (HESAM), Ecole Nationale d'Ingénieurs de Metz (ENIM), Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), and Centre Hospitalier Universitaire de Reims (CHU Reims)

Subjects
Computer science, Cyclic tensile characterization, [SDV]Life Sciences [q-bio], Biomedical Engineering, Failure, Umbilical Cord, Biomaterials, 030207 dermatology & venereal diseases, 03 medical and health sciences, 0302 clinical medicine, Biological property, Wharton's jelly, Ultimate tensile strength, Humans, Biomechanics, Wharton Jelly, Warthon’s jelly, Cells, Cultured, Cell Proliferation, 030304 developmental biology, 0303 health sciences, Viscosity, Hyperelasticity, Cell Differentiation, Mesenchymal Stem Cells, Numerical models, Predictive simulation, Tensile behavior, Mechanics of Materials, Biomedical engineering
Abstract

International audience; Wharton's jelly (WJ) is a mucous connective tissue of the umbilical cord. It shows high healing capabilities, mainly attributed to the chemical composition and to the presence of stem cells, growth factors and peptides. Although WJ biological properties are well documented in vitro and in vivo, there is still a lack of mechanical data on this tissue, which is paramount for its use as a biomaterial for medical applications. In this study, mechanical responses of ten WJ samples within close physiological conditions were registered undergoing quasi static cyclic tensile tests followed by a load up to failure. This protocol aimed on one hand to provide biomechanical data to feed predictive numerical models and on the other hand increase WJ knowledge in view of its potential use in biomedical field. In spite of the WJ harvest, the resulting viscous nonlinear elastic response obtained is fully in tune with the literature confirming the database quality. A side of the knowledge improvement on WJ mechanical response, this paper provides accurate data that will enhance predictive simulation work such as finite element analysis. The mechanical step-through brought by the analytical nonlinear characterization over cyclic and ultimate loads is to predict WJ behavior. Actually, principal component analysis highlighted its quality while pointing out indicators, such as failure or hydration criteria, as well as models' limitations.

Published
2022
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11. Bone marrow mesenchymal stem cells offer an immune-privileged niche to Cutibacterium acnes in case of implant-associated osteomyelitis

Author

Fany Reffuveille, Fabienne Quilès, Marie Dubus, Halima Kerdjoudj, S. Papa, Cédric Mauprivez, Julie Chevrier, R. Siboni, X. Ohl, J. Varin, Sophie C. Gangloff, Grégory Francius, Sandra Audonnet, Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Université de Reims Champagne-Ardenne (URCA), Laboratoire de Chimie Physique et Microbiologie pour les Matériaux et l'Environnement (LCPME), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Plateau Technique de Cytométrie en Flux (URCACyt), Pôle Odontologie [CHU Reims], Centre Hospitalier Universitaire de Reims (CHU Reims), and Colin, Marius

Subjects
Implant-associated infection, [SDV]Life Sciences [q-bio], Biomedical Engineering, Bone Marrow Cells, Bone healing, Biology, Biochemistry, Microbiology, Biomaterials, 03 medical and health sciences, Immune system, medicine, Humans, Macrophage, Secretion, Propionibacterium acnes, Molecular Biology, 030304 developmental biology, 0303 health sciences, 030306 microbiology, Intracellular parasite, Osteomyelitis, Mesenchymal stem cell, Biofilm, Prostheses and Implants, General Medicine, medicine.disease, 3. Good health, [SDV] Life Sciences [q-bio], Biofilms, Bone repair, Mesenchymal stem cells, Bacteria/ stem cells interactions, Cutibacterium acnes, Biotechnology
Abstract

Considered as some of the most devastating complications, Cutibacterium acnes (C. acnes)-related osteomyelitis are among the hardest infections to diagnose and treat. Mesenchymal stem cells (MSCs) secrete number of immunomodulatory and antimicrobial soluble factors, making them an attractive treatment for bacterial infection. In this study, we examined MSCs/C. acnes interaction and analyzed the subsequent MSCs and bacteria's behaviors. Human bone marrow-derived MSCs were infected by C. acnes clinical strain harvested from non-infected bone site. Following 3 h of interaction, around 4% of bacteria were found in the intracellular compartment. Infected MSCs increased the secretion of prostaglandin E2 and indolamine 2,3 dioxygenase immunomodulatory mediators. Viable intracellular bacteria analyzed by infrared spectroscopy and atomic force microscopy revealed deep modifications in the wall features. In comparison with unchallenged bacteria, the viable intracellular bacteria showed (i) an increase in biofilm formation on orthopaedical-based materials, (ii) an increase in the invasiveness of osteoblasts and (iii) persistence in macrophage, suggesting the acquisition of virulence factors. Overall, these results showed a direct impact of C. acnes on bone marrow-derived MSCs, suggesting that blocking the C. acnes/MSCs interactions may represent an important new approach to manage chronic osteomyelitis infections. Statement of significance The interaction of bone commensal C. acnes with bone marrow mesenchymal stem cells induces modifications in C. acnes wall characteristics. These bacteria increased (i) the biofilm formation on orthopaedical-based materials, (ii) the invasiveness of bone forming cells and (iii) the resistance to macrophage clearance through the modification of the wall nano-features and/or the increase in catalase production.

Published
2022
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12. Antibacterial and Immunomodulatory Properties of Acellular Wharton's Jelly Matrix

Author

Marie, Dubus, Loïc, Scomazzon, Julie, Chevrier, Charlotte, Ledouble, Adrien, Baldit, Julien, Braux, Florelle, Gindraux, Camille, Boulagnon, Sandra, Audonnet, Marius, Colin, Hassan, Rammal, Cédric, Mauprivez, and Halima, Kerdjoudj

Abstract

Of all biologic matrices, decellularized tissues have emerged as a promising tool in the field of regenerative medicine. Few empirical clinical studies have shown that Wharton's jelly (WJ) of the human umbilical cord promotes wound closure and reduces wound-related infections. In this scope, we herein investigated whether decellularized (DC)-WJ could be used as an engineered biomaterial. In comparison with devitalized (DV)-WJ, our results showed an inherent effect of DC-WJ on Gram positive (

Published
2021

13. Haemostatic sponges as an alternative to granular bone substitute for sinus lifts

Author

S. Okley, A. Baldit, P. Prada, Rachid Rahouadj, Cédric Mauprivez, Marie Dubus, Frédéric Velard, Halima Kerdjoudj, Laboratoire d'Etude des Microstructures et de Mécanique des Matériaux (LEM3), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Arts et Métiers Sciences et Technologies, HESAM Université (HESAM)-HESAM Université (HESAM), Ecole Nationale d'Ingénieurs de Metz (ENIM), Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)

Subjects
Bone substitute, microstructure, 0206 medical engineering, Biomedical Engineering, Sinus lift, Bioengineering, 02 engineering and technology, gelatin sponge, mechanical properties, [SPI.MECA.SOLID]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Solid mechanics [physics.class-ph], 03 medical and health sciences, 0302 clinical medicine, stomatognathic system, [SPI.MECA.MEMA]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Mechanics of materials [physics.class-ph], otorhinolaryngologic diseases, medicine, Sinus (anatomy), Dental alveolus, Gelatin sponge, business.industry, [SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph], 030229 sport sciences, General Medicine, Anatomy, 020601 biomedical engineering, [SPI.MECA.GEME]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Mechanical engineering [physics.class-ph], Computer Science Applications, Human-Computer Interaction, stomatognathic diseases, medicine.anatomical_structure, collagen sponge, Collagen sponge, [SPI.MECA.STRU]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Structural mechanics [physics.class-ph], business, Posterior maxilla
Abstract

International audience; Insufficient residual alveolar bone height is a common obstacle for the placement of dental implants in the posterior maxilla. Sinus lifting procedure involves the creation of a sub-sinus membrane pocket and the grafting with granular bone substitutes. Perforation of the sinus membrane is the most common complication with a large incidence rate (Testori et al. 2019). Graft material leak, through the perforation, results in a dental implant failure and/or postoperative sinusitis, requiring a complete reviewing of the maxillary sinus cavity and the removal of the remaining bone graft. The high perforation rate has led to develop new options involving 3 D biodegradable scaffolds instead of using granular bone substitutes. Case reports and case series have pointed out successful new bone formation after sinus membrane elevation using reoxidised cellulose (Gray et al. 2001), venous blood clot (Moon et al. 2011) and gelatin sponge (Sohn et al. 2010) as space maintainers. The main limitation of this promising technique was that the sub membrane space volume was limited and hardly predictable. The aim of this study was to evaluate the mechanical behaviour and volume kinematics of several haemostatic sponges to find out the best haemostatic medical device as space maintainers for new bone formation in sinus lift surgery. This study has been focused only on two devices: Gelita-spon® and Hemocollagene®. It aims to compare substitutes respectively made of porcine gelatine and bovine collagen.

Published
2020
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14. Mechanical behaviour of a membrane made of human umbilical cord for dental bone regenerative medicine

Author

Marie Dubus, Cédric Mauprivez, L. Scomazzon, Halima Kerdjoudj, Johan Sergheraert, R. Rahouadj, A. Baldit, Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Laboratoire d'Etude des Microstructures et de Mécanique des Matériaux (LEM3), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Arts et Métiers Sciences et Technologies, HESAM Université (HESAM)-HESAM Université (HESAM), and Ecole Nationale d'Ingénieurs de Metz (ENIM)

Subjects
0206 medical engineering, Biomedical Engineering, Dentistry, Mechanical properties, Bioengineering, 02 engineering and technology, [SPI.MECA.SOLID]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Solid mechanics [physics.class-ph], Umbilical cord, Regenerative medicine, 03 medical and health sciences, 0302 clinical medicine, stomatognathic system, Periodontal disease, Bio-Gide®, [SPI.MECA.MEMA]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Mechanics of materials [physics.class-ph], medicine, hyperelasticity, Bone regeneration, business.industry, [SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph], 030229 sport sciences, General Medicine, 020601 biomedical engineering, [SPI.MECA.GEME]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Mechanical engineering [physics.class-ph], Computer Science Applications, Human-Computer Interaction, stomatognathic diseases, medicine.anatomical_structure, guided bone regeneration, [SPI.MECA.STRU]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Structural mechanics [physics.class-ph], human umbilical cord, business
Abstract

International audience; Guided bone regeneration (GBR) is one of the most attractive techniques for restoring oral bone defects following tooth extraction or periodontal disease. To ensure the stability of a dental implant, the GBR technique relies on the use of an occlusive membrane, which is positioned as a barrier over the bone defect, providing space maintenance required for bone growth and preventing the ingrowth of fibrous tissue into the bone defect (Elgali et al. 2017). This membrane has to meet criteria guidelines in terms of mechanical properties, as the membrane has to allow a good handling for dental surgeons, without collapsing into the bone defect. Healthy perinatal tissues are promising biomaterials because they are inexpensive, and universally available (Ferguson and Dodson 2009). Among these tissues, the human umbilical cord, mainly composed of collagen fibres and glycosaminoglycan, especially hyaluronic acid and chondroitin sulphate, is expected to offer outstanding opportunities for tissue engineering by serving as a suitable biocompatible membrane for GBR.In this study, a novel membrane derived from the human umbilical cord (UC-membrane) was successfully developed following tissue stripping and freeze-drying processes. Ice crystal formation following tissue freezing is known to induce pore formation within the tissue, hampering their mechanical properties. Herein, the mechanical behaviour of UC-membrane was determined and compared to the Bio-Gide® membrane (gold standard membrane)

Published
2020
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15. Biomimetic Hierarchical Structuring of PLA by Ultra-Short Laser Pulses for Processing of Tissue Engineered Matrices: Study of Cellular and Antibacterial Behavior

Author

Halima Kerdjoudj, L. Angelova, A. Daskalova, Ivan Buchvarov, Emil Filipov, Julie Chevrier, Dante Aceti, Rosica Mincheva, Xavier Carrete, A. Trifonov, Marie Dubus, Institute of Electronics [Sofia], Bulgarian Academy of Sciences (BAS), Laboratory of Polymeric and Composite Materials, Université de Mons-Hainaut (LPCM), Université de Mons-Hainaut, Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Université de Reims Champagne-Ardenne (URCA), and Faculty of Physics, Sofia University saint Kliment Ohridski

Subjects
Materials science, Polymers and Plastics, Scanning electron microscope, [SDV]Life Sciences [q-bio], FOS: Physical sciences, Organic chemistry, 02 engineering and technology, Surface finish, Article, 03 medical and health sciences, QD241-441, X-ray photoelectron spectroscopy, temporal scaffolds, Surface roughness, Physics - Biological Physics, Fourier transform infrared spectroscopy, PLA texturing, 030304 developmental biology, 0303 health sciences, cell matrices, General Chemistry, respiratory system, 021001 nanoscience & nanotechnology, Microstructure, Chemical engineering, Biological Physics (physics.bio-ph), ultra-short functionalization of cell matrices, tissue engineering, Surface modification, Fs bioscaffolds structuring, Wetting, 0210 nano-technology
Abstract

The influence of ultra-short laser modification on the surface morphology and possible chemical alteration of poly-lactic acid (PLA) matrix in respect to the optimization of cellular and antibacterial behavior were investigated in this study. Scanning electron microscopy (SEM) morphological examination of the processed PLA surface showed the formation of diverse hierarchical surface microstructures, generated by irradiation with a range of laser fluences (F) and scanning velocities (V) values. By controlling the laser parameters, diverse surface roughness can be achieved, thus influencing cellular dynamics. This surface feedback can be applied to finely tune and control diverse biomaterial surface properties like wettability, reflectivity, and biomimetics. The triggering of thermal effects, leading to the ejection of material with subsequent solidification and formation of raised rims and 3D-like hollow structures along the processed zones, demonstrated a direct correlation to the wettability of the PLA. A transition from superhydrophobic (θ &gt, 150°) to super hydrophilic (θ &lt, 20°) surfaces can be achieved by the creation of grooves with V = 0.6 mm/s, F = 1.7 J/cm2. The achieved hierarchical architecture affected morphology and thickness of the processed samples which were linked to the nature of ultra-short laser-material interaction effects, namely the precipitation of temperature distribution during material processing can be strongly minimized with ultrashort pulses leading to non-thermal and spatially localized effects that can facilitate volume ablation without collateral thermal damage The obtained modification zones were analyzed employing Fourier transform infrared (FTIR), X-ray photoelectron spectroscopy (XPS), Energy dispersive X-ray analysis (EDX), and optical profilometer. The modification of the PLA surface resulted in an increased roughness value for treatment with lower velocities (V = 0.6 mm/s). Thus, the substrate gains a 3D-like architecture and forms a natural matrix by microprocessing with V = 0.6 mm/s, F = 1.7 J/cm2, and V = 3.8 mm/s, F = 0.8 J/cm2. The tests performed with Mesenchymal stem cells (MSCs) demonstrated that the ultra-short laser surface modification altered the cell orientation and promoted cell growth. The topographical design was tested also for the effectiveness of bacterial attachment concerning chosen parameters for the creation of an array with defined geometrical patterns.

Published
2021
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16. Biopolymers-calcium phosphate antibacterial coating reduces the pathogenicity of internalized bacteria by mesenchymal stromal cells

Author

Halima Alem, Halima Kerdjoudj, Marie Dubus, Fany Reffuveille, Fouzia Boulmedais, Hassan Rammal, Jennifer Varin-Simon, Pierre Prada, Cédric Mauprivez, Loïc Scomazzon, Boulmedais, Fouzia, Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Institut Jean Lamour (IJL), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Institut Charles Sadron (ICS), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Université de Lorraine (UL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Matériaux et nanosciences d'Alsace (FMNGE), and Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, Calcium Phosphates, Staphylococcus aureus, Stromal cell, media_common.quotation_subject, [SDV]Life Sciences [q-bio], education, Biomedical Engineering, 02 engineering and technology, medicine.disease_cause, Microbiology, 03 medical and health sciences, Biopolymers, [CHIM] Chemical Sciences, medicine, [CHIM]Chemical Sciences, General Materials Science, Internalization, ComputingMilieux_MISCELLANEOUS, media_common, biology, Virulence, Chemistry, Mesenchymal stem cell, Biofilm, Mesenchymal Stem Cells, Bone prosthesis, 021001 nanoscience & nanotechnology, biology.organism_classification, Anti-Bacterial Agents, [SDV] Life Sciences [q-bio], 030104 developmental biology, medicine.anatomical_structure, Bone marrow, 0210 nano-technology, Bacteria
Abstract

A multifunctional material system that kills bacteria and drives bone healing is urgently sought to improve bone prosthesis. Herein, the osteoinductive coating made of calcium phosphate/chitosan/hyaluronic acid, named Hybrid, was proposed as an antibacterial substrate for stromal cell adhesion. This Hybrid coating possesses a contact-killing effect reducing by 90% the viability of Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Pseudomonas aeruginosa (P. aeruginosa) strains after 48 h of contact. In addition to the production of immunomodulatory mediators, Wharton's jelly (WJ-SCs), dental pulp (DPSCs) and bone marrow (BM-MSCs) derived stromal cells were able to release antibacterial and antibiofilm agents effective against S. aureus and P. aeruginosa strains, respectively. Studying the effect of the Hybrid coating on the internalization of S. aureus by the stromal cells, in acute-mimicking bone infection, highlighted an increase in the bacteria internalization by DPSCs and BM-MSCs when cultured on the Hybrid coating versus uncoated glass. Despite the internalization, Hybrid coating showed a beneficial effect by reducing the pathogenicity of the internalized bacteria. The formation of biofilm was reduced by at least 50% in comparison to internalized bacteria by stromal cells on uncoated glass. This work opens the route for the development of innovative antibacterial coatings by taking into account the internalization of bacteria by stromal cells.

Published
2020
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17. Interaction of Cutibacterium acnes with human bone marrow derived mesenchymal stem cells: a step toward understanding bone implant- associated infection development

Author

Marie Dubus, Céline Mongaret, Cédric Mauprivez, Halima Kerdjoudj, J. Varin, Julie Chevrier, Sophie C. Gangloff, Hassan Rammal, E. Maisonneuve, Fany Reffuveille, S. Papa, Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)

Subjects
Adult, Prosthesis-Related Infections, Neutrophils, 0206 medical engineering, Cell, Biomedical Engineering, Virulence, Bone Marrow Cells, 02 engineering and technology, Biology, Biochemistry, Bone and Bones, Microbiology, Immunomodulation, Biomaterials, Immune system, medicine, Humans, Secretion, Molecular Biology, Tissue homeostasis, ComputingMilieux_MISCELLANEOUS, Aged, Cell Death, Intracellular parasite, Mesenchymal stem cell, Mesenchymal Stem Cells, General Medicine, Middle Aged, Propionibacteriaceae, 021001 nanoscience & nanotechnology, 020601 biomedical engineering, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Anti-Bacterial Agents, medicine.anatomical_structure, Biofilms, Culture Media, Conditioned, Bone marrow, 0210 nano-technology, Biotechnology
Abstract

Crosstalk between mesenchymal stem cells (MSCs) and bacteria plays an important role in regulating the regenerative capacities of MSCs, fighting infections, modulating immune responses and maintaining tissue homeostasis. Commensal Cutibacterium acnes (C. acnes) bacterium becomes an opportunistic pathogen causing implant-associated infections. Herein, we examined MSCs/C. acnes interaction and analysed the subsequent bacteria and MSCs behaviours following infection. Human bone marrow derived MSCs were infected by two clinical and one laboratory C. acnes strains. Following 3h of interaction, all bacterial strains were able to invade MSCs. Viable intracellular bacteria acquired virulence factors by increasing biofilm formation and/or by affecting macrophage phagocytosis. Although the direct and indirect (through neutrophil stimulation) antibacterial effects of the MSCs secretome were not enhanced following C. acnes infection, ELISA analysis revealed that C. acnes clinical strains are able to license MSCs to become immunosuppressive cell-like by increasing the secretion of IL-6, IL-8, PGE-2, VEGF, TGF-β and HGF. Overall, these results showed a direct impact of C. acnes on bone marrow derived MSCs, providing new insights into the development of C. acnes during implant-associated infections. STATEMENT OF SIGNIFICANCE: The originality of this work relies on the study of relationship between human bone marrow derived mesenchymal stem cells (MSCs) phenotype and C. acnes clinical strains virulence following cell infection. Our major results showed that C. acnes are able to invade MSCs, inducing a transition of commensal to an opportunistic pathogen behaviour. Although the direct and indirect antibacterial effects were not enhanced following C. acnes infection, secretome analysis revealed that C. acnes clinical strains were able to license MSCs to become immunosuppressive and anti-fibrotic cell-like. These results showed a direct impact of C. acnes on bone marrow derived MSCs, providing new insights into the development of C. acnes during associated implant infections.

Published
2020
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18. Bioinspired Nanofeatured Substrates: Suitable Environment for Bone Regeneration

Author

Fany Reffuveille, Fouzia Boulmedais, Christine Terryn, Fabienne Quilès, Grégory Francius, Halima Alem, Hassan Rammal, Halima Kerdjoudj, Dominique Laurent-Maquin, Pierre Schaaf, Sophie C. Gangloff, Marie Dubus, L. Aubert, Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Université de Reims Champagne-Ardenne (URCA), Plateforme en Imagerie Cellulaire et Tissulaire (PICT), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Institut Charles Sadron (ICS), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Biomatériaux et Bioingénierie (BB), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Jean Lamour (IJL), Université de Lorraine (UL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie Physique et Microbiologie pour l'Environnement (LCPME), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Matériaux et nanosciences d'Alsace, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Université de Strasbourg (UNISTRA), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et nanosciences d'Alsace, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Université de Strasbourg (UNISTRA)-Réseau nanophotonique et optique, Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Université de Strasbourg (UNISTRA), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie Physique et Microbiologie pour les Matériaux et l'Environnement (LCPME), Hocquel, Romain, Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Vascular Endothelial Growth Factor A, 0301 basic medicine, Bone Regeneration, Materials science, Surface Properties, [SDV]Life Sciences [q-bio], Aucun, 02 engineering and technology, Bone healing, engineering.material, Bone tissue, 03 medical and health sciences, Mechanobiology, Coated Materials, Biocompatible, Osteoprotegerin, Osteogenesis, [CHIM] Chemical Sciences, medicine, [CHIM]Chemical Sciences, General Materials Science, Bone regeneration, ComputingMilieux_MISCELLANEOUS, Osteoblasts, Regeneration (biology), 021001 nanoscience & nanotechnology, Nanostructures, [SDV] Life Sciences [q-bio], Durapatite, 030104 developmental biology, medicine.anatomical_structure, engineering, Biophysics, Biopolymer, Stem cell, 0210 nano-technology
Abstract

Bone mimicking coatings provide a complex microenvironment in which material, through its inherent properties (such as nanostructure and composition), affects the commitment of stem cells into bone lineage and the production of bone tissue regulating factors required for bone healing and regeneration. Herein, a bioactive mineral/biopolymer composite made of calcium phosphate/chitosan and hyaluronic acid (CaP-CHI-HA) was elaborated using a versatile simultaneous spray coating of interacting species. The resulting CaP-CHI-HA coating was mainly constituted of bioactive, carbonated and crystalline hydroxyapatite with 277 ± 98 nm of roughness, 1 μm of thickness, and 2.3 ± 1 GPa of stiffness. After five days of culture, CaP-CHI-HA suggested a synergistic effect of intrinsic biophysical features and biopolymers on stem cell mechanobiology and nuclear organization, leading to the expression of an early osteoblast-like phenotype and the production of bone tissue regulating factors such as osteoprotegerin and vascular endothelial growth factor. More interestingly, amalgamation with biopolymers conferred to the mineral a bacterial antiadhesive property. These significant data shed light on the potential regenerative application of CaP-CHI-HA bioinspired coating in providing a suitable environment for stem cell bone regeneration and an ideal strategy to prevent implant-associated infections. journal article 2017 Apr 12 2017 03 30 imported

Published
2017
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19. Boosting mesenchymal stem cells regenerative activities on biopolymers calcium phosphate functionalized collagen membrane

Author

Marie Dubus, Hassan Rammal, Halima Alem, Nicolas Bogdan Bercu, Isabelle Royaud, Fabienne Quilès, Fouzia Boulmedais, Gangloff, Sophie C., Cédric Mauprivez, Halima KERDJOUDJ, Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Institut Jean Lamour (IJL), Centre National de la Recherche Scientifique (CNRS)-Université de Lorraine (UL), Laboratoire de Recherche en Nanosciences - EA 4682 (LRN), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie Physique et Microbiologie pour les Matériaux et l'Environnement (LCPME), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Institut Charles Sadron (ICS), Matériaux et nanosciences d'Alsace, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Université de Strasbourg (UNISTRA)-Réseau nanophotonique et optique, and Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)

Subjects
[SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2019

20. Boosting mesenchymal stem cells regenerative activities on biopolymers-calcium phosphate functionalized collagen membrane

Author

Halima Kerdjoudj, Halima Alem, Cédric Mauprivez, Fouzia Boulmedais, Isabelle Royaud, Sophie C. Gangloff, Hassan Rammal, Marie Dubus, N.B. Bercu, Fabienne Quilès, Université de Reims Champagne-Ardenne (URCA), Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Institut Jean Lamour (IJL), Université de Lorraine (UL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Recherche en Nanosciences - EA 4682 (LRN), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Laboratoire de Chimie Physique et Microbiologie pour les Matériaux et l'Environnement (LCPME), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Institut Charles Sadron (ICS), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Hôpital Maison Blanche, and Centre Hospitalier Universitaire de Reims (CHU Reims)

Subjects
Calcium Phosphates, Bone Regeneration, Surface Properties, [SDV]Life Sciences [q-bio], Aucun, 02 engineering and technology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Bone tissue, 01 natural sciences, Bone resorption, chemistry.chemical_compound, Colloid and Surface Chemistry, Biopolymers, 0103 physical sciences, Hyaluronic acid, medicine, Humans, Physical and Theoretical Chemistry, Particle Size, Bone regeneration, Octacalcium phosphate, ComputingMilieux_MISCELLANEOUS, Cells, Cultured, 010304 chemical physics, Mesenchymal stem cell, Mesenchymal Stem Cells, Surfaces and Interfaces, General Medicine, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, Cell biology, Endothelial stem cell, Membrane, medicine.anatomical_structure, chemistry, Collagen, 0210 nano-technology, Biotechnology
Abstract

The regeneration of bone-soft tissue interface, using functional membranes, remains challenging and can be promoted by improving mesenchymal stem cells (MSCs) paracrine function. Herein, a collagen membrane, used as guided bone regeneration membrane, was functionalized by calcium phosphate, chitosan and hyaluronic acid hybrid coating by simultaneous spray of interacting species process. Composed of brushite, octacalcium phosphate and hydroxyapatite, the hybrid coating increased the membrane stiffness by 50%. After 7 days of MSCs culture on the hybrid coated polymeric membrane, biological studies were marked by a lack of osteoblastic commitment. However, MSCs showed an enhanced proliferation along with the secretion of cytokines and growth factors that could block bone resorption and favour endothelial cell recruitment without exacerbating polynuclear neutrophils infiltration. These data shed light on the great potential of inorganic/organic coated collagen membranes as an alternative bioactive factor-like platform to improve MSCs regenerative capacity, in particular to support bone tissue vascularization and to modulate inflammatory infiltrates. journal article 2019 Sep 01 2019 06 15 imported

Published
2019
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21. Probing the pH dependent assembly-disassembly of water-soluble organic capsules with coumarins and anthracene

Author

Sree Gayathri Talluri, Barnali Mondal, A. Mohan Raj, V. Ramamurthy, Shipra Gupta, Marie Dubus, and Université de Reims Champagne-Ardenne (URCA)

Subjects
Anthracene, 010405 organic chemistry, General Chemical Engineering, General Physics and Astronomy, Ph dependent, General Chemistry, 010402 general chemistry, Photochemistry, Excimer, Coumarin, 01 natural sciences, Fluorescence, 0104 chemical sciences, chemistry.chemical_compound, Monomer, chemistry, Assembly disassembly, Molecule, [CHIM]Chemical Sciences, heterocyclic compounds, ComputingMilieux_MISCELLANEOUS
Abstract

Octaacid (OA) and octaamine (OAm) form water-soluble capsules in presence of guest molecules. Using the polarity dependent fluorescence of coumarins (coumarin-1, coumarin-153 and coumarin-480) we have shown that the dynamic OA and OAm capsules can be reversibly disassembled and reassembled by controlling the pH of the solution. Anthracene is a unique guest molecule that forms a 2:2 capsule with both OA and OAm and shows excimer emission when inside the capsule and monomer emission when in water. Reversible pH dependent excimer-monomer emission of anthracene has revealed that the disassembly process is much dependent on the hydrophobicity of the guest. The disassembly of the capsules is slower with anthracene than with the less hydrophobic coumarins. In addition the assembly-disassembly is not 100% with hydrophobic anthracene while quantitative in the case of coumarins.

Published
2018
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22. Suivi de la différenciation des cellules souches en ostéoblastes par des techniques d’imagerie

Author

Marie Dubus, Bour, C., Aubert, L., Christine Terryn, Halima Alem, Fabienne Quilès, Gangloff, Sophie C., Cédric Mauprivez, Halima KERDJOUDJ, Hassan Rammal, Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Plateforme en Imagerie Cellulaire et Tissulaire (PICT), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Institut Jean Lamour (IJL), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie Physique et Microbiologie pour l'Environnement (LCPME), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), UFR Odontologie, Université Paris Diderot - Paris 7 (UPD7), Université de Lorraine (UL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and QUILES, Fabienne

Subjects
[SDV] Life Sciences [q-bio], [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, [CHIM.THEO] Chemical Sciences/Theoretical and/or physical chemistry, [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2017

23. Collagen-Based Medical Device as a Stem Cell Carrier for Regenerative Medicine

Author

Camille Boulagnon-Rombi, Marie Dubus, Roselyne Garnotel, Cédric Mauprivez, Céline Mongaret, Halima Kerdjoudj, Camille Bour, Frédéric Velard, Céline Schneider, Sophie C. Gangloff, Rachid Rahouadj, L. Aubert, Cédric Laurent, Hassan Rammal, Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Université de Reims Champagne-Ardenne (URCA), Centre Hospitalier Universitaire de Reims (CHU Reims), Hôpital universitaire Robert Debré [Reims], Matrice extracellulaire et dynamique cellulaire - UMR 7369 (MEDyC), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Groupe d'Étude sur les Géomatériaux et Environnements Naturels, Anthropiques et Archéologiques - EA 3795 (GEGENAA), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Maison des Sciences Humaines de Champagne-Ardenne (MSH-URCA), Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA), Laboratoire Énergies et Mécanique Théorique et Appliquée (LEMTA ), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Pharmacologie et Toxicologie [CHU Reims], Laboratoire d'anatomie et cytologie pathologiques [Reims], and Laurent, Cédric

Subjects
0301 basic medicine, [PHYS.MECA.VIBR] Physics [physics]/Mechanics [physics]/Vibrations [physics.class-ph], Scaffold, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, stem cell niche, medical device, regenerative medicine, biocompatibility, paracrine activities, [PHYS.MECA.MSMECA] Physics [physics]/Mechanics [physics]/Materials and structures in mechanics [physics.class-ph], 02 engineering and technology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SPI.MAT] Engineering Sciences [physics]/Materials, Regenerative medicine, [SPI.MAT]Engineering Sciences [physics]/Materials, lcsh:Chemistry, [PHYS.MECA.MEMA]Physics [physics]/Mechanics [physics]/Mechanics of materials [physics.class-ph], Tissue culture, [PHYS.MECA.STRU]Physics [physics]/Mechanics [physics]/Structural mechanics [physics.class-ph], [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [PHYS.MECA.SOLID]Physics [physics]/Mechanics [physics]/Solid mechanics [physics.class-ph], lcsh:QH301-705.5, Spectroscopy, ComputingMilieux_MISCELLANEOUS, [PHYS.MECA.SOLID] Physics [physics]/Mechanics [physics]/Solid mechanics [physics.class-ph], [PHYS.MECA.VIBR]Physics [physics]/Mechanics [physics]/Vibrations [physics.class-ph], General Medicine, 021001 nanoscience & nanotechnology, 3. Good health, Computer Science Applications, Cell biology, Cellular Microenvironment, Collagen, Stem cell, 0210 nano-technology, Type I collagen, Biocompatibility, Preservation, Biological, Nanotechnology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Biology, Article, Catalysis, Inorganic Chemistry, 03 medical and health sciences, [PHYS.MECA.MEMA] Physics [physics]/Mechanics [physics]/Mechanics of materials [physics.class-ph], [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Humans, Physical and Theoretical Chemistry, [PHYS.MECA.BIOM]Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph], [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials, Molecular Biology, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, [PHYS.MECA.STRU] Physics [physics]/Mechanics [physics]/Structural mechanics [physics.class-ph], Organic Chemistry, Mesenchymal stem cell, [PHYS.MECA.BIOM] Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph], [SDV.BA.MVSA] Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, Mesenchymal Stem Cells, [PHYS.MECA.MSMECA]Physics [physics]/Mechanics [physics]/Materials and structures in mechanics [physics.class-ph], [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.IB.BIO] Life Sciences [q-bio]/Bioengineering/Biomaterials, Transplantation, [SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging, 030104 developmental biology, lcsh:Biology (General), lcsh:QD1-999
Abstract

Maintenance of mesenchymal stem cells (MSCs) requires a tissue-specific microenvironment (i.e., niche), which is poorly represented by the typical plastic substrate used for two-dimensional growth of MSCs in a tissue culture flask. The objective of this study was to address the potential use of collagen-based medical devices (HEMOCOLLAGENE®, Saint-Maur-des-Fossés, France) as mimetic niche for MSCs with the ability to preserve human MSC stemness in vitro. With a chemical composition similar to type I collagen, HEMOCOLLAGENE® foam presented a porous and interconnected structure (>90%) and a relative low elastic modulus of around 60 kPa. Biological studies revealed an apparently inert microenvironment of HEMOCOLLAGENE® foam, where 80% of cultured human MSCs remained viable, adopted a flattened morphology, and maintained their undifferentiated state with basal secretory activity. Thus, three-dimensional HEMOCOLLAGENE® foams present an in vitro model that mimics the MSC niche with the capacity to support viable and quiescent MSCs within a low stiffness collagen I scaffold simulating Wharton’s jelly. These results suggest that haemostatic foam may be a useful and versatile carrier for MSC transplantation for regenerative medicine applications.

Published
2017
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27. Bioinspired calcium phosphate/chitosan and hyaluronic acid substrates for bone regeneration

Author

Hassan Rammal, Marie Dubus, Aubert, L., Christine Terryn, Fanny Reffuveille, Halima Alem, Fabienne Quilès, Gangloff, Sophie C., Fouzia Boulmedais, Halima KERDJOUDJ, Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Plateforme en Imagerie Cellulaire et Tissulaire (PICT), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Institut Jean Lamour (IJL), Université de Lorraine (UL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie Physique et Microbiologie pour l'Environnement (LCPME), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Institut Charles Sadron (ICS), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), and Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

28. Combining Calcium Phosphates with Polysaccharides: A Bone-Inspired Material Modulating Monocyte/Macrophage Early Inflammatory Response

Author

Rammal, Hassan, Bour, Camille, Dubus, Marie, Entz, Laura, Aubert, Léa, Gangloff, Sophie, Audonnet, Sandra, Bercu, Nicolae, Boulmedais, Fouzia, Mauprivez, Cédric, Kerdjoudj, Halima, Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Université de Reims Champagne-Ardenne (URCA), Plateau Technique de Cytométrie en Flux (URCACyt), Laboratoire de Recherche en Nanosciences - EA 4682 (LRN), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Institut Charles Sadron (ICS), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Hôpital Maison Blanche, Centre Hospitalier Universitaire de Reims (CHU Reims), Physiopathologie, Pharmacologie et Ingénierie articulaires (PPIA), Université Henri Poincaré - Nancy 1 (UHP)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Hydrologie et de Géochimie de Strasbourg (LHyGeS), Ecole et Observatoire des Sciences de la Terre (EOST), Université de Strasbourg (UNISTRA)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École Nationale du Génie de l'Eau et de l'Environnement de Strasbourg (ENGEES)-Centre National de la Recherche Scientifique (CNRS), Institut Charles Sadron (Strasbourg), UFR Odontologie, Université Paris Diderot - Paris 7 (UPD7), DUBUS, MARIE, Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), and This work was partially supported by «Interreg V France-Wallonie-Vlaanderen program-TEXTOS ».Marie Dubus is supported by a doctoral fellowship from the High Education French Minister.

Subjects
Calcium Phosphates, Vascular Endothelial Growth Factor A, THP-1 Cells, [SDV]Life Sciences [q-bio], Aucun, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Bone and Bones, Article, lcsh:Chemistry, bone regeneration, Transforming Growth Factor beta, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], Humans, bone inspired material, Hyaluronic Acid, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, lcsh:QH301-705.5, Chemokine CCL2, ComputingMilieux_MISCELLANEOUS, Chitosan, Tumor Necrosis Factor-alpha, Interleukins, Vinculin, Mitochondria, Hyaluronan Receptors, Gene Expression Regulation, lcsh:Biology (General), lcsh:QD1-999, inflammation, Bone Substitutes, monocyte, cytotoxicity, Reactive Oxygen Species, Receptors, Calcium-Sensing, Signal Transduction
Abstract

The use of inorganic calcium/phosphate supplemented with biopolymers has drawn lots of attention in bone regenerative medicine. While inflammation is required for bone healing, its exacerbation alters tissue regeneration/implants integration. Inspired by bone composition, a friendly automated spray-assisted system was used to build bioactive and osteoinductive calcium phosphate/chitosan/hyaluronic acid substrate (CaP-CHI-HA). Exposing monocytes to CaP-CHI-HA resulted in a secretion of pro-healing VEGF and TGF-&beta, growth factors, TNF-&alpha, MCP-1, IL-6 and IL-8 pro-inflammatory mediators but also IL-10 anti-inflammatory cytokine along with an inflammatory index below 1.5 (versus 2.5 and 7.5 following CaP and LPS stimulation, respectively). Although CD44 hyaluronic acid receptor seems not to be involved in the inflammatory regulation, results suggest a potential role of chemical composition and calcium release from build-up substrates, in affecting the intracellular expression of a calcium-sensing receptor. Herein, our findings indicate a great potential of CaP-CHI-HA in providing required inflammation-healing balance, favorable for bone healing/regeneration.

Published
2018
Full Text
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