69 results on '"Marie Carriere"'
Search Results
2. Perinatal foodborne titanium dioxide exposure-mediated dysbiosis predisposes mice to develop colitis through life
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Caroline Carlé, Delphine Boucher, Luisa Morelli, Camille Larue, Ekaterina Ovtchinnikova, Louise Battut, Kawthar Boumessid, Melvin Airaud, Muriel Quaranta-Nicaise, Jean-Luc Ravanat, Gilles Dietrich, Sandrine Menard, Gérard Eberl, Nicolas Barnich, Emmanuel Mas, Marie Carriere, Ziad Al Nabhani, and Frédérick Barreau
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Perinatal period ,Foodborne TiO2 ,Intestinal barrier function ,Intestinal stem cells ,Microbiota ,Colitis ,Toxicology. Poisons ,RA1190-1270 ,Industrial hygiene. Industrial welfare ,HD7260-7780.8 - Abstract
Abstract Background Perinatal exposure to titanium dioxide (TiO2), as a foodborne particle, may influence the intestinal barrier function and the susceptibility to develop inflammatory bowel diseases (IBD) later in life. Here, we investigate the impact of perinatal foodborne TiO2 exposure on the intestinal mucosal function and the susceptibility to develop IBD-associated colitis. Pregnant and lactating mother mice were exposed to TiO2 until pups weaning and the gut microbiota and intestinal barrier function of their offspring was assessed at day 30 post-birth (weaning) and at adult age (50 days). Epigenetic marks was studied by DNA methylation profile measuring the level of 5-methyl-2′-deoxycytosine (5-Me-dC) in DNA from colic epithelial cells. The susceptibility to develop IBD has been monitored using dextran-sulfate sodium (DSS)-induced colitis model. Germ-free mice were used to define whether microbial transfer influence the mucosal homeostasis and subsequent exacerbation of DSS-induced colitis. Results In pregnant and lactating mice, foodborne TiO2 was able to translocate across the host barriers including gut, placenta and mammary gland to reach embryos and pups, respectively. This passage modified the chemical element composition of foetus, and spleen and liver of mothers and their offspring. We showed that perinatal exposure to TiO2 early in life alters the gut microbiota composition, increases the intestinal epithelial permeability and enhances the colonic cytokines and myosin light chain kinase expression. Moreover, perinatal exposure to TiO2 also modifies the abilities of intestinal stem cells to survive, grow and generate a functional epithelium. Maternal TiO2 exposure increases the susceptibility of offspring mice to develop severe DSS-induced colitis later in life. Finally, transfer of TiO2-induced microbiota dysbiosis to pregnant germ-free mice affects the homeostasis of the intestinal mucosal barrier early in life and confers an increased susceptibility to develop colitis in adult offspring. Conclusions Our findings indicate that foodborne TiO2 consumption during the perinatal period has negative long-lasting consequences on the development of the intestinal mucosal barrier toward higher colitis susceptibility. This demonstrates to which extent environmental factors influence the microbial-host interplay and impact the long-term mucosal homeostasis.
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- 2023
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3. Microstructured Magnetoelastic Membrane for Magnetic Bioactuators and Soft Artificial Muscles Applications
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Svetlana Ponomareva, Marie Carriere, Yanxia Hou, Robert Morel, Bernard Dieny, and Hélène Joisten
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magnetic actuation ,magnetic bioactuators ,magnetic microdisks ,magnetoelastic membranes ,mechanobiology ,microstructured composite polymers ,Computer engineering. Computer hardware ,TK7885-7895 ,Control engineering systems. Automatic machinery (General) ,TJ212-225 - Abstract
In the growing field of mechanobiology, artificial mechano‐reactive systems play an essential role in the generation of mechanical forces and control of material deformations. Free‐standing magnetic nanoparticles have been studied for the mechanical stimulation of living cells. Magnetic composite materials are also used to mimic muscles at macroscale. In this study, a new magnetically actuated membrane is focused, which can be used for various applications in soft robotics or as a bioreactor. It consists of a few microns thick polydimethylsiloxane (PDMS) membrane in which an array of magnetic microdisks is embedded. These membranes have a large tuneable flexibility, and they are transparent, biocompatible, and waterproof. They are usable in biology and optics, both potentially combined. The membrane deformations under magnetic field have been experimentally characterized and modeled. By growing pancreatic cells on such membranes, it has been demonstrated that insulin production from the cells can be enhanced thanks to the mechanical stimulation of the cells provided by the actuated membrane.
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- 2023
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4. Changes of physico-chemical properties of nano-biomaterials by digestion fluids affect the physiological properties of epithelial intestinal cells and barrier models
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Giulia Antonello, Arianna Marucco, Elena Gazzano, Panagiotis Kainourgios, Costanza Ravagli, Ana Gonzalez-Paredes, Simone Sprio, Esperanza Padín-González, Mahmoud G. Soliman, David Beal, Francesco Barbero, Paolo Gasco, Giovanni Baldi, Marie Carriere, Marco P. Monopoli, Costas A. Charitidis, Enrico Bergamaschi, Ivana Fenoglio, and Chiara Riganti
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Nano-biomaterials ,In vitro simulated digestion ,Biotransformation ,Toxicity ,Caco-2 ,HCT116 ,Toxicology. Poisons ,RA1190-1270 ,Industrial hygiene. Industrial welfare ,HD7260-7780.8 - Abstract
Abstract Background The widespread use of nano-biomaterials (NBMs) has increased the chance of human exposure. Although ingestion is one of the major routes of exposure to NBMs, it is not thoroughly studied to date. NBMs are expected to be dramatically modified following the transit into the oral-gastric-intestinal (OGI) tract. How these transformations affect their interaction with intestinal cells is still poorly understood. NBMs of different chemical nature—lipid-surfactant nanoparticles (LSNPs), carbon nanoparticles (CNPs), surface modified Fe3O4 nanoparticles (FNPs) and hydroxyapatite nanoparticles (HNPs)—were treated in a simulated human digestive system (SHDS) and then characterised. The biological effects of SHDS-treated and untreated NBMs were evaluated on primary (HCoEpiC) and immortalised (Caco-2, HCT116) epithelial intestinal cells and on an intestinal barrier model. Results The application of the in vitro SDHS modified the biocompatibility of NBMs on gastrointestinal cells. The differences between SHDS-treated and untreated NBMs could be attributed to the irreversible modification of the NBMs in the SHDS. Aggregation was detected for all NBMs regardless of their chemical nature, while pH- or enzyme-mediated partial degradation was detected for hydroxyapatite or polymer-coated iron oxide nanoparticles and lipid nanoparticles, respectively. The formation of a bio-corona, which contains proteases, was also demonstrated on all the analysed NBMs. In viability assays, undifferentiated primary cells were more sensitive than immortalised cells to digested NBMs, but neither pristine nor treated NBMs affected the intestinal barrier viability and permeability. SHDS-treated NBMs up-regulated the tight junction genes (claudin 3 and 5, occludin, zonula occludens 1) in intestinal barrier, with different patterns between each NBM, and increase the expression of both pro- and anti-inflammatory cytokines (IL-1β, TNF-α, IL-22, IL-10). Notably, none of these NBMs showed any significant genotoxic effect. Conclusions Overall, the results add a piece of evidence on the importance of applying validated in vitro SHDS models for the assessment of NBM intestinal toxicity/biocompatibility. We propose the association of chemical and microscopic characterization, SHDS and in vitro tests on both immortalised and primary cells as a robust screening pipeline useful to monitor the changes in the physico-chemical properties of ingested NBMs and their effects on intestinal cells.
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- 2022
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5. Hazard, Distribution and Exposure of Particulate Pollution from Indoor and Outdoor Environments
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Maurizio Gualtieri, Marie Carriere, and Paride Mantecca
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n/a ,Chemical technology ,TP1-1185 - Abstract
Air is an essential natural resource for life [...]
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- 2023
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6. Nanomaterial genotoxicity evaluation using the high-throughput p53-binding protein 1 (53BP1) assay.
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Maelle Fontaine, Eline Bartolami, Marion Prono, David Béal, Magda Blosi, Anna L Costa, Costanza Ravagli, Giovanni Baldi, Simone Sprio, Anna Tampieri, Ivana Fenoglio, Lang Tran, Bengt Fadeel, and Marie Carriere
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Medicine ,Science - Abstract
Toxicity evaluation of engineered nanomaterials is challenging due to the ever increasing number of materials and because nanomaterials (NMs) frequently interfere with commonly used assays. Hence, there is a need for robust, high-throughput assays with which to assess their hazard potential. The present study aimed at evaluating the applicability of a genotoxicity assay based on the immunostaining and foci counting of the DNA repair protein 53BP1 (p53-binding protein 1), in a high-throughput format, for NM genotoxicity assessment. For benchmarking purposes, we first applied the assay to a set of eight known genotoxic agents, as well as X-ray irradiation (1 Gy). Then, a panel of NMs and nanobiomaterials (NBMs) was evaluated with respect to their impact on cell viability and genotoxicity, and to their potential to induce reactive oxygen species (ROS) production. The genotoxicity recorded using the 53BP1 assay was confirmed using the micronucleus assay, also scored via automated (high-throughput) microscopy. The 53BP1 assay successfully identified genotoxic compounds on the HCT116 human intestinal cell line. None of the tested NMs showed any genotoxicity using the 53BP1 assay, except the positive control consisting in (CoO)(NiO) NMs, while only TiO2 NMs showed positive outcome in the micronucleus assay. Only Fe3O4 NMs caused significant elevation of ROS, not correlated to DNA damage. Therefore, owing to its adequate predictivity of the genotoxicity of most of the tested benchmark substance and its ease of implementation in a high throughput format, the 53BP1 assay could be proposed as a complementary high-throughput screening genotoxicity assay, in the context of the development of New Approach Methodologies.
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- 2023
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7. Titanium dioxide particles from the diet: involvement in the genesis of inflammatory bowel diseases and colorectal cancer
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Frédérick Barreau, Céline Tisseyre, Sandrine Ménard, Audrey Ferrand, and Marie Carriere
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TiO2 ,Food additive ,Toxicity ,Gastrointestinal tract ,Ingestion ,Intestine ,Toxicology. Poisons ,RA1190-1270 ,Industrial hygiene. Industrial welfare ,HD7260-7780.8 - Abstract
Abstract The gastrointestinal tract is a complex interface between the external environment and the immune system. Its ability to control uptake across the mucosa and to protect the body from damage of harmful substances from the lumen is defined as the intestinal barrier function (IBF). The IBF involves four elements: the intestinal microbiota, the mucus layer, the epithelium and the immune system. Its dysfunction is linked with human diseases including inflammatory, metabolic, infectious, autoimmune and neurologic disorders. Most of these diseases are complex and involve genetic, psychological and environmental factors. Over the past 10 years, many genetic polymorphisms predisposing to inflammatory bowel disease (IBD) have been identified. Yet, it is now clear that they are insufficient to explain the onset of these chronic diseases. Although it has been evidenced that some environmental factors such as cigarette smoking or carbohydrate intake are associated with IBD, other environmental factors also present potential health risks such as ingestion of food additives introduced in the human diet, including those composed of mineral particles, by altering the four elements of the intestinal barrier function. The aim of this review is to provide a critical opinion on the potential of TiO2 particles, especially when used as a food additive, to alter the four elements of the intestinal barrier function, and consequently to evaluate if this additive would likely play a role in the development and/or exacerbation of IBD.
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- 2021
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8. Physicochemical Transformations of Silver Nanoparticles in the Oro-Gastrointestinal Tract Mildly Affect Their Toxicity to Intestinal Cells In Vitro: An AOP-Oriented Testing Approach
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Ozge Kose, David Béal, Sylvie Motellier, Nathalie Pelissier, Véronique Collin-Faure, Magda Blosi, Rossella Bengalli, Anna Costa, Irini Furxhi, Paride Mantecca, and Marie Carriere
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silver nanoparticles ,Ag NP ,intestine ,toxicity ,simulated gastrointestinal fluids ,in vitro digestion ,Chemical technology ,TP1-1185 - Abstract
The widespread use of silver nanoparticles (Ag NPs) in food and consumer products suggests the relevance of human oral exposure to these nanomaterials (NMs) and raises the possibility of adverse effects in the gastrointestinal tract. The aim of this study was to investigate the toxicity of Ag NPs in a human intestinal cell line, either uncoated or coated with polyvinylpyrrolidone (Ag PVP) or hydroxyethylcellulose (Ag HEC) and digested in simulated gastrointestinal fluids. Physicochemical transformations of Ag NPs during the different stages of in vitro digestion were identified prior to toxicity assessment. The strategy for evaluating toxicity was constructed on the basis of adverse outcome pathways (AOPs) showing Ag NPs as stressors. It consisted of assessing Ag NP cytotoxicity, oxidative stress, genotoxicity, perturbation of the cell cycle and apoptosis. Ag NPs caused a concentration-dependent loss of cell viability and increased the intracellular level of reactive oxygen species as well as DNA damage and perturbation of the cell cycle. In vitro digestion of Ag NPs did not significantly modulate their toxicological impact, except for their genotoxicity. Taken together, these results indicate the potential toxicity of ingested Ag NPs, which varied depending on their coating but did not differ from that of non-digested NPs.
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- 2023
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9. Air–Liquid Interface Exposure of Lung Epithelial Cells to Low Doses of Nanoparticles to Assess Pulmonary Adverse Effects
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Silvia Diabaté, Lucie Armand, Sivakumar Murugadoss, Marco Dilger, Susanne Fritsch-Decker, Christoph Schlager, David Béal, Marie-Edith Arnal, Mathilde Biola-Clier, Selina Ambrose, Sonja Mülhopt, Hanns-Rudolf Paur, Iseult Lynch, Eugenia Valsami-Jones, Marie Carriere, and Carsten Weiss
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cerium dioxide ,zirconium-doping ,titanium dioxide ,nanotoxicology ,alternative methods ,Chemistry ,QD1-999 - Abstract
Reliable and predictive in vitro assays for hazard assessments of manufactured nanomaterials (MNMs) are still limited. Specifically, exposure systems which more realistically recapitulate the physiological conditions in the lung are needed to predict pulmonary toxicity. To this end, air-liquid interface (ALI) systems have been developed in recent years which might be better suited than conventional submerged exposure assays. However, there is still a need for rigorous side-by-side comparisons of the results obtained with the two different exposure methods considering numerous parameters, such as different MNMs, cell culture models and read outs. In this study, human A549 lung epithelial cells and differentiated THP-1 macrophages were exposed under submerged conditions to two abundant types of MNMs i.e., ceria and titania nanoparticles (NPs). Membrane integrity, metabolic activity as well as pro-inflammatory responses were recorded. For comparison, A549 monocultures were also exposed at the ALI to the same MNMs. In the case of titania NPs, genotoxicity was also investigated. In general, cells were more sensitive at the ALI compared to under classical submerged conditions. Whereas ceria NPs triggered only moderate effects, titania NPs clearly initiated cytotoxicity, pro-inflammatory gene expression and genotoxicity. Interestingly, low doses of NPs deposited at the ALI were sufficient to drive adverse outcomes, as also documented in rodent experiments. Therefore, further development of ALI systems seems promising to refine, reduce or even replace acute pulmonary toxicity studies in animals.
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- 2020
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10. Biotransformation of Food-Grade and Nanometric TiO2 in the Oral–Gastro–Intestinal Tract: Driving Forces and Effect on the Toxicity toward Intestinal Epithelial Cells
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Arianna Marucco, Marion Prono, David Beal, Enrica Alasonati, Paola Fisicaro, Enrico Bergamaschi, Marie Carriere, and Ivana Fenoglio
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food ,TiO2 ,intestinal cells ,size ,surface ,bio-corona ,Chemistry ,QD1-999 - Abstract
Background: Oral exposure to titanium dioxide (TiO2) is common since it is widely used in food and pharmaceutical products. Concern on the safety of this substance has been recently raised, due to the presence of an ultrafine fraction in food-grade TiO2. Discrepancy exists among data reported in in vitro and in vivo studies on intestinal acute/chronic toxicity of TiO2. This might be due to the different biological identity of TiO2 in traditional in vitro test by respect in vivo conditions. Methods: One food-grade TiO2 and two nanometric TiO2 samples were treated with a simulated human digestive dystem (SHDS) in order to investigate the bio-transformation occurring to the particles once ingested in term of size distribution (Dynamic Light Scattering—DLS-, Flow Particle Imaging, Asymmetric Flow Field Flow Fractionation-AF4-) and surface modification (Electrophoretic Light Scattering—ELS-, Electron Paramagnetic Resonance Spectroscopy—EPR-). The effect of SHDS on the cyto-, genotoxicity and potential to induce oxidative stress towards human colorectal carcinoma HCT116 cells was also assessed. Results: Aggregation as a consequence of the high ionic strength of the gastric and intestinal simulated fluids was observed, together with the formation of a partially irreversible bio-corona containing phosphate ions and proteins. Such bio-corona led to a partial masking of the TiO2 particles surface and reactivity. Pristine and treated TiO2 nanoparticles showed comparable acute toxicity and genotoxicity toward HCT116 cells, whereas a small decrease of the induction of oxidative stress after treatment was observed. Conclusions: Overall the results underline the importance of SHDS as a tool to improve the predictive power of in vitro tests towards intestinal nanomaterial toxicity.
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- 2020
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11. Toxicity to RAW264.7 Macrophages of Silica Nanoparticles and the E551 Food Additive, in Combination with Genotoxic Agents
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Fanny Dussert, Pierre-Adrien Arthaud, Marie-Edith Arnal, Bastien Dalzon, Anaëlle Torres, Thierry Douki, Nathalie Herlin, Thierry Rabilloud, and Marie Carriere
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silica ,SiO2 ,nanoparticle ,E551 ,toxicity ,genotoxicity ,Chemistry ,QD1-999 - Abstract
Synthetic amorphous silica (SAS) is used in a plethora of applications and included in many daily products to which humans are exposed via inhalation, ingestion, or skin contact. This poses the question of their potential toxicity, particularly towards macrophages, which show specific sensitivity to this material. SAS represents an ideal candidate for the adsorption of environmental contaminants due to its large surface area and could consequently modulate their toxicity. In this study, we assessed the toxicity towards macrophages and intestinal epithelial cells of three SAS particles, either isolated SiO2 nanoparticles (LS30) or SiO2 particles composed of agglomerated-aggregates of fused primary particles, either food-grade (E551) or non-food-grade (Fumed silica). These particles were applied to cells either alone or in combination with genotoxic co-contaminants, i.e., benzo[a]pyrene (B[a]P) and methane methylsulfonate (MMS). We show that macrophages are much more sensitive to these toxic agents than a non-differenciated co-culture of Caco-2 and HT29-MTX cells, used here as a model of intestinal epithelium. Co-exposure to SiO2 and MMS causes DNA damage in a synergistic way, which is not explained by the modulation of DNA repair protein mRNA expression. Together, this suggests that SiO2 particles could adsorb genotoxic agents on their surface and, consequently, increase their DNA damaging potential.
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- 2020
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12. Titanium Dioxide Nanoparticles Alter the Cellular Phosphoproteome in A549 Cells
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Mathilde Biola-Clier, Jean-Charles Gaillard, Thierry Rabilloud, Jean Armengaud, and Marie Carriere
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omics ,lung ,inhalation ,nanoparticle ,tio2 ,proteomics ,phosphoproteomics ,Chemistry ,QD1-999 - Abstract
TiO2 nanoparticles (NPs) are one of the most produced NPs worldwide and are used in many consumer products. Their impact on human health, especially through inhalation, has been studied for more than two decades. TiO2 is known for its strong affinity towards phosphates, and consequently interaction with cellular phosphates may be one of the mechanisms driving its toxicity. In the present study, we used a phosphoproteomics approach to document the interaction of TiO2-NP with phosphoproteins from A549 human pulmonary alveolar epithelial cells. Cells were exposed to 21 nm anatase/rutile TiO2-NPs, then their phosphopeptides were extracted and analyzed using shotgun proteomics. By comparing the phosphoprotein content, phosphorylation status and phosphorylation sites of exposed cells with that of control cells, our results show that by affecting the phosphoproteome, TiO2-NPs affect cellular processes such as apoptosis, linked with cell cycle and the DNA damage response, TP53 being central to these pathways. Other pathways including inflammation and molecular transport are also affected. These molecular mechanisms of TiO2-NP toxicity have been reported previously, our study shows for the first time that they may derive from phosphoproteome modulation, which could be one of their upstream regulators.
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- 2020
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13. Uranium interaction with two multi-resistant environmental bacteria: Cupriavidus metallidurans CH34 and Rhodopseudomonas palustris.
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Isabelle Llorens, Guillaume Untereiner, Danielle Jaillard, Barbara Gouget, Virginie Chapon, and Marie Carriere
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Medicine ,Science - Abstract
Depending on speciation, U environmental contamination may be spread through the environment or inversely restrained to a limited area. Induction of U precipitation via biogenic or non-biogenic processes would reduce the dissemination of U contamination. To this aim U oxidation/reduction processes triggered by bacteria are presently intensively studied. Using X-ray absorption analysis, we describe in the present article the ability of Cupriavidus metallidurans CH34 and Rhodopseudomonas palustris, highly resistant to a variety of metals and metalloids or to organic pollutants, to withstand high concentrations of U and to immobilize it either through biosorption or through reduction to non-uraninite U(IV)-phosphate or U(IV)-carboxylate compounds. These bacterial strains are thus good candidates for U bioremediation strategies, particularly in the context of multi-pollutant or mixed-waste contaminations.
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- 2012
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14. Complementary in vitro and in vivo strategies to assess the biological effects of the nano enabled food additives E171 and E551
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Ana Peropadre, Patricia Vega-Cuesta, Paloma Fernández Freire, Diego Pulido, Marie Carriere, and Jose F. de Celis
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Materials Science (miscellaneous) ,General Environmental Science - Abstract
Exposure to non-cytotoxic concentrations of E551 and E172 causes similar changes in the expression levels of genes related to the regulation of oxidative stress and DNA integrity in Drosophila intestines and human differentiated Caco-2 cells.
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- 2023
15. Magnetic particles for triggering insulin release in INS-1E cells subjected to a rotating magnetic field
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Svetlana Ponomareva, Helene Joisten, Taina François, Cecile Naud, Robert Morel, Yanxia Hou, Thomas Myers, Isabelle Joumard, Bernard Dieny, Marie Carriere, SPINtronique et TEchnologie des Composants (SPINTEC), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Chimie Interface Biologie pour l’Environnement, la Santé et la Toxicologie (CIBEST ), SYstèmes Moléculaires et nanoMatériaux pour l’Energie et la Santé (SYMMES), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Chimie pour la Reconnaissance et l’Etude d’Assemblages Biologiques (CREAB ), The IBS Electron Microscope facility is supported by the Auvergne Rhône-Alpes Region, the Fonds Feder, the Fondation pour la Recherche Médicale, and GIS-IBiSA., This work used the EM facility and flow cytometry platform at the Grenoble Instruct-ERIC Center (ISBG, UMS 3518 CNRS CEA-UGA-EMBL), ANR-10-INBS-0005,FRISBI,Infrastructure Française pour la Biologie Structurale Intégrée(2010), ANR-17-EURE-0003,CBH-EUR-GS,CBH-EUR-GS(2017), and European Project: 665440,H2020,H2020-FETOPEN-2014-2015-RIA,ABIOMATER(2015)
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Glucose ,Magnetic Fields ,Polymers ,Insulin-Secreting Cells ,Magnetic Phenomena ,Insulin ,General Materials Science ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology - Abstract
International audience; Diabetes is a major global health threat. Both academics and industry are striving to develop effective treatments for this disease. In this work, we present a new approach to induce insulin release from β-islet pancreatic cells (INS-1E) by mechanical stimulation. Two types of experiments were carried out. First, a local stimulation was performed by dispersing anisotropic magnetic particles within the cell medium, which settled down almost immediately on cell plasma membranes. Application of a low frequency magnetic field (up to 40 Hz) generated by a custom-made magnetic device resulted in oscillations of these particles, which then exerted a mechanical constraint on the cell plasma membranes. The second type of experiment consisted of a global stimulation, where cells were grown on magneto-elastic membranes composed of a biocompatible polymer with embedded magnetic particles. Upon application of a rotating magnetic field, magnetic particles within the membrane were attracted towards the field source, resulting in the membrane's vibrations being transmitted to the cells grown on it. In both experiments, the cell response to these mechanical stimulations caused by application of the variable magnetic field was quantified via the measurement of insulin release in the growth medium. We demonstrated that the mechanical action induced by the motion of magnetic particles or by membrane vibrations was an efficient stimulus for insulin granule secretion from β-cells. This opens a wide range of possible applications including the design of a system which triggers insulin secretion by β-islet pancreatic cells on demand.
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- 2022
16. FRET-based nanosensor AMPfret distinguishes physiological from toxic stress
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Uwe Schlattner, Roland Abi Nahed, Francesco Aulicino, Alaa Al Assi, Eric Fontaine, Marie Carriere, and Imre Berger
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Biophysics - Published
- 2023
17. Selective plane illumination microscope dedicated to volumetric imaging in microfluidic chambers
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Caroline Bissardon, Xavier Mermet, Clément Quintard, Federico Sanjuan, Yves Fouillet, Frédéric Bottausci, Marie Carriere, Florence Rivera, Pierre Blandin, Département Microtechnologies pour la Biologie et la Santé (DTBS), Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratoire des Fluides Complexes et leurs Réservoirs (LFCR), TOTAL FINA ELF-Université de Pau et des Pays de l'Adour (UPPA)-Centre National de la Recherche Scientifique (CNRS), Chimie Interface Biologie pour l’Environnement, la Santé et la Toxicologie (CIBEST ), SYstèmes Moléculaires et nanoMatériaux pour l’Energie et la Santé (SYMMES), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)
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[PHYS]Physics [physics] ,[SDV]Life Sciences [q-bio] ,Atomic and Molecular Physics, and Optics ,Article ,Biotechnology - Abstract
International audience; In this article, we are presenting an original selective plane illumination fluorescence microscope dedicated to image “Organ-on-chip”-like biostructures in microfluidic chips. In order to be able to morphologically analyze volumetric samples in development at the cellular scale inside microfluidic chambers, the setup presents a compromise between relatively large field of view (∼ 200 µm) and moderate resolution (∼ 5 µm). The microscope is based on a simple design, built around the chip and its microfluidic environment to allow 3D imaging inside the chip. In particular, the sample remains horizontally avoiding to disturb the fluidics phenomena. The experimental setup, its optical characterization and the first volumetric images are reported.
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- 2022
18. Intracellular Localization of an Osmocenyl‐Tamoxifen Derivative in Breast Cancer Cells Revealed by Synchrotron Radiation X‐ray Fluorescence Nanoimaging
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Florin Fus, Yang Yang, Hui Zhi Shirley Lee, Siden Top, Marie Carriere, Alexandre Bouron, Alexandra Pacureanu, Julio Cesar da Silva, Michèle Salmain, Anne Vessières, Peter Cloetens, Gérard Jaouen, and Sylvain Bohic
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General Medicine - Published
- 2019
19. Evaluation of the Dermal Toxicity of InZnP Quantum Dots Before and After Accelerated Weathering: Toward a Safer-By-Design Strategy
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Fanny, Dussert, Karl David, Wegner, Christine, Moriscot, Benoit, Gallet, Pierre-Henri, Jouneau, Peter, Reiss, and Marie, Carriere
- Abstract
Quantum dots (QDs) are colloidal fluorescent semiconductor nanocrystals with exceptional optical properties. Their widespread use, particularly in light-emitting diodes (LEDs), displays, and photovoltaics, is questioning their potential toxicity. The most widely used QDs are CdSe and CdTe QDs, but due to the toxicity of cadmium (Cd), their use in electrical and electronic equipment is now restricted in the European Union through the Restriction of hazardous substances in electrical and electronic equipment (RoHS) directive. This has prompted the development of safer alternatives to Cd-based QDs; among them, InP QDs are the most promising ones. We recently developed RoHS-compliant QDs with an alloyed core composed of InZnP coated with a Zn(Se,S) gradient shell, which was further coated with an additional ZnS shell to protect the QDs from oxidative surface degradation. In this study, the toxicity of single-shelled InZnP/Zn(Se,S) core/gradient shell and of double-shelled InZnP/Zn(Se,S)/ZnS core/shell/shell QDs was evaluated both in their pristine form and after aging in a climatic chamber, mimicking a realistic environmental weathering. We show that both pristine and aged QDs, whatever their composition, accumulate in the cytoplasm of human primary keratinocytes where they form agglomerates at the vicinity of the nucleus. Pristine QDs do not show overt toxicity to cells, while aged QDs show cytotoxicity and genotoxicity and significantly modulate the mRNA expression of proteins involved in zinc homeostasis, cell redox response, and inflammation. While the three aged QDs show similar toxicity, the toxicity of pristine gradient-shell QD is higher than that of pristine double-shell QD, confirming that adding a second shell is a promising safer-by-design strategy. Taken together, these results suggest that end-of-life degradation products from InP-based QDs are detrimental to skin cells in case of accidental exposure and that the mechanisms driving this effect are oxidative stress, inflammation, and disturbance of cell metal homeostasis, particularly Zn homeostasis. Further efforts to promote safer-by-design formulations of QDs, for instance by reducing the In and Zn content and/or implementing a more robust outer shell, are therefore warranted.
- Published
- 2020
20. TiO
- Author
-
Marie, Carriere, Marie-Edith, Arnal, and Thierry, Douki
- Subjects
Titanium ,Mutagenicity Tests ,Animals ,Humans ,DNA ,DNA Damage ,Mutagens - Abstract
TiO
- Published
- 2019
21. All the Feels / Tous Les Sens : Affect and Writing in Canada / Affect Et Écriture Au Canada
- Author
-
Marie Carrière, Ursula Mathis-Moser, Kit Dobson, Marie Carrière, Ursula Mathis-Moser, and Kit Dobson
- Subjects
- Canadian literature--21st century--History and criticism, French-Canadian literature--21st century--History and criticism, Affect (Psychology) in literature, Cognition in literature
- Abstract
All the Feels / Tous les sens presents research into emotion and cognition in Canadian, Indigenous, and Québécois writings in English or French. Affect is both internal and external, private and public; with its fluid boundaries, it represents a productive dimension for literary analysis. The emerging field of affect studies makes vital claims about ethical impulses, social justice, and critical resistance, and thus much is at stake when we adopt affective reading practices. The contributors ask what we can learn from reading contemporary literatures through this lens. Unique and timely, readable and teachable, this collection is a welcome resource for scholars of literature, feminism, philosophy, and transnational studies as well as anyone who yearns to imagine the world differently. Contributors: Nicole Brossard, Marie Carrière, Matthew Cormier, Kit Dobson, Nicoletta Dolce, Louise Dupré, Margery Fee, Ana María Fraile-Marcos, Smaro Kamboureli, Aaron Kreuter, Daniel Laforest, Carmen Mata Barreiro, Ursula Mathis-Moser, Heather Milne, Eric Schmaltz, Maïté Snauwaert, Jeanette den Toonder
- Published
- 2021
22. Cautiously Hopeful : Metafeminist Practices in Canada
- Author
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Marie Carrière and Marie Carrière
- Subjects
- Feminist theory, Feminist theory--Canada, Intersectionality (Sociology), Canadian literature--21st century--History and criticism, Feminism in literature, Feminism, Feminism--Canada
- Abstract
If feminism has always been characterized by its divisions, it is metafeminism, a term coined by Lori Saint-Martin, that defines and embraces that disorder. As a carefully devised reading practice, metafeminism understands contemporary feminist literature and theory as both recalling and extending the tropes and politics of the past. In Cautiously Hopeful Marie Carrière brings together seemingly disparate writing by Anglo-Canadian, Indigenous, and Québécois women authors under the banner of metafeminism.Familiarizing readers with major streams of feminist thought, including intersectionality, affect theory, and care ethics, Carrière shows how literary works by such authors as Dionne Brand, Nicole Brossard, Naomi Fontaine, Larissa Lai, Tracey Lindberg, and Rachel Zolf, among others, tackle the entanglement of gender with race, settler-invader colonialism, heteronormativity, positionality, language, and the posthuman condition. Meanwhile tenable alliances among Indigenous women, women of colour, and settler feminist practitioners emerge. Carrière's tone is personal and accessible throughout - in itself a metafeminist gesture that both encompasses and surpasses a familiar feminist form of writing.Despite the growing anti-feminist backlash across media platforms and in various spheres of political and social life, a hopefulness animates this timely work that, like metafeminism, stands alert to the challenges that feminism faces in its capacity to effect social change in the twenty-first century.
- Published
- 2020
23. Writing Beyond the End Times? / Écrire Au-delà De La Fin Des Temps ? : The Literatures of Canada and Quebec / Les Littératures Au Canada Et Au Québec
- Author
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Ursula Mathis-Moser, Marie Carrière, Ursula Mathis-Moser, and Marie Carrière
- Subjects
- Canadian literature--21st century--History and criticism, Canadian literature--21st century--Themes, motives, Social change in literature, Crises in literature, Apocalypse in literature
- Abstract
This collection of essays examines how the sense of crisis that occasionally seems to overwhelm us directs and transforms Canadian and Quebec writings in English and French, and conversely, how literature and criticism set out to counterbalance the social, economic, and ideological insecurities we live in. Ce recueil de textes étudie les manières dont le sentiment de crise qui peut parfois sembler nous submerger, oriente et transforme les écrits canadiens et québécois d'expressions anglaise et française, et inversement, comment la littérature et la critique s'efforcent de contrebalancer les insécurités sociales, économiques et idéologiques dans lesquelles nous vivons. Contributors: David Boucher, Marie Carrière, Nicole Côté, Piet Defraeye, Nicoletta Dolce, Danielle Dumontet, Ana María Fraile-Marcos, Marion Kühn, Hans-Jürgen Lüsebrink, Carmen Mata Barreiro, Ursula Mathis-Moser, Dunja M. Mohr, Émilie Notard, Daniel Poitras, Véronique Porra, Srilata Ravi, Marion Christina Rohrleitner
- Published
- 2019
24. Toxicological impact of acute exposure to E171 food additive and TiO
- Author
-
Marie, Dorier, Céline, Tisseyre, Fanny, Dussert, David, Béal, Marie-Edith, Arnal, Thierry, Douki, Vanessa, Valdiglesias, Blanca, Laffon, Sónia, Fraga, Fátima, Brandão, Nathalie, Herlin-Boime, Frédérick, Barreau, Thierry, Rabilloud, and Marie, Carriere
- Subjects
Titanium ,Cell Survival ,DNA, Neoplasm ,Endoplasmic Reticulum Stress ,Coculture Techniques ,Gene Expression Regulation, Neoplastic ,Oxidative Stress ,8-Hydroxy-2'-Deoxyguanosine ,Humans ,Food Additives ,RNA, Messenger ,RNA, Neoplasm ,Caco-2 Cells ,Particle Size ,Reactive Oxygen Species ,Tumor Suppressor p53-Binding Protein 1 ,HT29 Cells - Abstract
TiO
- Published
- 2018
25. Dispersion of nanomaterials in aqueous media: Towards protocol optimization
- Author
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Eugenia Valsami-Jones, Steffi Rades, Annegret Potthoff, Wolfgang Unger, Caterina Minelli, Martine Mayne-L'Hermite, Soline Allard, Marie Carriere, Ratna Tantra, Isabella Romer, Laura-Jayne Ellis, Inder Kaur, Publica, School of Geography, Earth and Environmental Sciences [Birmingham], University of Birmingham [Birmingham], National Physical Laboratory [Teddington] (NPL), Chimie Interface Biologie pour l’Environnement, la Santé et la Toxicologie (CIBEST ), SYstèmes Moléculaires et nanoMatériaux pour l’Energie et la Santé (SYMMES), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratoire Edifices Nanométriques (LEDNA), Nanosciences et Innovation pour les Matériaux, la Biomédecine et l'Energie (ex SIS2M) (NIMBE UMR 3685), Institut Rayonnement Matière de Saclay (IRAMIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Rayonnement Matière de Saclay (IRAMIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), BAM Federal Institute for Materials Research and Testing, Federal Institute for Materials Research and Testing - Bundesanstalt für Materialforschung und -prüfung (BAM), Fraunhofer Institute for Ceramic Technologies and Systems (Fraunhofer IKTS), Fraunhofer (Fraunhofer-Gesellschaft), European Project: 263147,EC:FP7:NMP,FP7-NMP-2010-LARGE-4,NANOVALID(2011), Institut de Chimie du CNRS (INC)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Institut Rayonnement Matière de Saclay (IRAMIS), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)
- Subjects
sonication ,General Immunology and Microbiology ,General Chemical Engineering ,General Neuroscience ,Water ,[CHIM.MATE]Chemical Sciences/Material chemistry ,General Biochemistry, Genetics and Molecular Biology ,Issue 130 ,Nanostructures ,optimization Date ,protocol optimization ,dispersion ,characterization ,optimization ,Environmental Sciences ,Nanomaterials - Abstract
International audience; The sonication process is commonly used for de-agglomerating and dispersing nanomaterials in aqueous based media, necessary to improve homogeneity and stability of the suspension. In this study, a systematic step-wise approach is carried out to identify optimal sonication conditions in order to achieve a stable dispersion. This approach has been adopted and shown to be suitable for several nanomaterials (cerium oxide, zinc oxide, and carbon nanotubes) dispersed in deionized (DI) water. However, with any change in either the nanomaterial type or dispersing medium, there needs to be optimization of the basic protocol by adjusting various factors such as sonication time, power, and sonicator type as well as temperature rise during the process. The approach records the dispersion process in detail. This is necessary to identify the time points as well as other above-mentioned conditions during the sonication process in which there may be undesirable changes, such as damage to the particle surface thus affecting surface properties. Our goal is to offer a harmonized approach that can control the quality of the final, produced dispersion. Such a guideline is instrumental in ensuring dispersion quality repeatability in the nanoscience community, particularly in the field of nanotoxicology.
- Published
- 2017
26. Putative adverse outcome pathways for silver nanoparticle toxicity on mammalian male reproductive system: a literature review
- Author
-
Ozge Kose, Paride Mantecca, Anna Costa, and Marie Carrière
- Subjects
Adverse outcome pathways ,Silver nanoparticles ,Reproductive toxicity ,Molecular initiating event ,Key events ,Adverse outcome ,Toxicology. Poisons ,RA1190-1270 ,Industrial hygiene. Industrial welfare ,HD7260-7780.8 - Abstract
Abstract Background Adverse outcome pathways (AOPs) are conceptual frameworks that organize knowledge about biological interactions and toxicity mechanisms. They present a sequence of events commencing with initial interaction(s) of a stressor, which defines the perturbation in a biological system (molecular initiating event, MIE), and a dependent series of key events (KEs), ending with an adverse outcome (AO). AOPs have recently become the subject of intense studies in a view to better understand the mechanisms of nanomaterial (NM) toxicity. Silver nanoparticles (Ag NPs) are one of the most explored nanostructures and are extensively used in various application. This, in turn, has increased the potential for interactions of Ag NPs with environments, and toxicity to human health. The aim of this study was to construct a putative AOPs (pAOP) related to reproductive toxicity of Ag NPs, in order to lay the groundwork for a better comprehension of mechanisms affecting both undesired toxicity (against human cell) and expected toxicity (against microorganisms). Methods PubMed and Scopus were systematically searched for peer-reviewed studies examining reproductive toxicity potential of Ag NPs. The quality of selected studies was assessed through ToxRTool. Eventually, forty-eight studies published between 2005 and 2022 were selected to identify the mechanisms of Ag NPs impact on reproductive function in human male. The biological endpoints, measurements, and results were extracted from these studies. Where possible, endpoints were assigned to a potential KE and an AO using expert judgment. Then, KEs were classified at each major level of biological organization. Results We identified the impairment of intracellular SH-containing biomolecules, which are major cellular antioxidants, as a putative MIE, with subsequent KEs defined as ROS accumulation, mitochondrial damage, DNA damage and lipid peroxidation, apoptosis, reduced production of reproductive hormones and reduced quality of sperm. These successive KEs may result in impaired male fertility (AO). Conclusion This research recapitulates and schematically represents complex literature data gathered from different biological levels and propose a pAOP related to the reproductive toxicity induced by AgNPs. The development of AOPs specific to NMs should be encouraged in order to provide new insights to gain a better understanding of NP toxicity.
- Published
- 2023
- Full Text
- View/download PDF
27. Ten Canadian Writers in Context
- Author
-
Marie Carrière, Curtis Gillespie, Jason Purcell, Marie Carrière, Curtis Gillespie, and Jason Purcell
- Subjects
- Canadian literature--21st century, Canadian literature--21st century--History and criticism, Authorship
- Abstract
Ten years, ten authors, ten critics. The Canadian Literature Centre/Centre de littérature canadienne reaches into its ten-year archive of Brown Bag Lunch readings to sample some of the most diverse and powerful voices in contemporary Canadian literature. This anthology offers readers samples from some of Canada's most exciting writers of fiction, nonfiction, and poetry. Each selection is introduced by a brief essay, serving as a point of entry into the writer's work. From the east coast of Newfoundland to Kitamaat territory on British Columbia's central coast, there is a story for everyone, from everywhere. True to Canada's multilingual and multicultural heritage, these ten writers come from diverse ethnicities and backgrounds, and work in multiple languages, including English, French, and Cree. Ying Chen | essay by Julie Rodgers Lynn Coady | essay by Maïté Snauwaert Michael Crummey | essay by Jennifer Bowering Delisle Caterina Edwards | essay by Joseph Pivato Marina Endicott | essay by Daniel Laforest Lawrence Hill | essay by Winfried Siemerling Alice Major | essay by Don Perkins Eden Robinson | essay by Kit Dobson Gregory Scofield | essay by Angela Van Essen Kim Thúy | essay by Pamela V. Sing
- Published
- 2016
28. Some Aspects of the Nature and Pedagogy of Academic Formation: How Can Academic Formation be Useful for Apostolic Life and Pastoral Ministry?
- Author
-
S.J. Jean-Marie Carriere
- Subjects
Pedagogy ,Christian ministry ,Sociology - Published
- 2012
29. Les prévisions opérationnelles de précipitations à Météo-France
- Author
-
Jean-Christophe Vincendon and Jean-Marie Carriere
- Subjects
Water Science and Technology - Abstract
Cet article decrit tres brievement la prevision operationnelle des precipitations a Meteo-France, pour toutes les echeances allant de la prevision immediate a la prevision a moyenne echeance reposant sur les systemes de prevision d'ensemble.
- Published
- 2009
30. El misterio de la cruz : Cuaderno Bíblico 166
- Author
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Jean-François Baudoz, Jean-Marie Carrière, Roselyne Dupont-Roc, Christophe Raimbault, Jean-François Baudoz, Jean-Marie Carrière, Roselyne Dupont-Roc, and Christophe Raimbault
- Abstract
Tuvieron que pasar cinco siglos para que los cristianos osaran representar a Jesús crucificado. Muerte infame y discurso paradójico a la vez, el Mesías crucificado es'escándalo para los judíos y locura para los paganos'(1 Cor 1,23), tanto actualmente como ayer. Pablo, Marcos, y, luego, todos los demás, lo catalogan de Buena Noticia para todos, de'Evangelio'. En el concilio de Nicea, en 325, los obispos heredarán su reflexión y forjarán las palabras que se encuentran en el centro del Credo:'Crucificado bajo el poder de Poncio Pilato, sufrió su pasión y fue sepultado'.
- Published
- 2014
31. Regenerations / Régénérations : Canadian Women's Writing / Écriture Des Femmes Au Canada
- Author
-
Marie Carrière, Patricia Demers, Marie Carrière, and Patricia Demers
- Subjects
- Criticism, interpretation, etc, Canadian literature--Women authors--History an, French-Canadian literature--Women authors--His, Canadian literature (English)--Women authors--, Canadian literature (French)--Women authors--H, Canadian literature--Women authors, French-Canadian literature--Women authors
- Abstract
Buttressed by a wealth of new, collaborative research methods and technologies, the contributors of this collection examine women's writing in Canada, past and present, with 11 essays in English and 5 in French. Regenerations was born out of the inaugural conference of the Canadian Writing Research Collaboratory held at the Canadian Literature Centre, University of Alberta, and exemplifies the progress of radically interdisciplinary research, collaboration, and publishing efforts surrounding Canadian women's writing. Researchers and students interested in Canadian literature, Québec literature, women's writing, literary history, feminist theory, and digital humanities scholarship should definitely acquaint themselves with this work. Contributors: Nicole Brossard, Susan Brown, Marie Carrière, Patricia Demers, Louise Dennys, Cinda Gault, Lucie Hotte, Dean Irvine, Gary Kelly, Shauna Lancit, Mary McDonald-Rissanen, Lindsey McMaster, Mary-Jo Romaniuk, Julie Roy, Susan Rudy, Chantal Savoie, Maïté Snauwaert, Rosemary Sullivan, and Sheena Wilson.
- Published
- 2014
32. Long-term exposure of A549 cells to titanium dioxide nanoparticles induces DNA damage and sensitizes cells towards genotoxic agents
- Author
-
Lucie Armand, Adeline Tarantini, David Beal, Mathilde Biola-Clier, Laure Bobyk, Sephanie Sorieul, Karin Pernet-Gallay, Caroline Marie-Desvergne, Iseult Lynch, Nathalie Herlin-Boime, Marie Carriere, Lucie Armand, Adeline Tarantini, David Beal, Mathilde Biola-Clier, Laure Bobyk, Sephanie Sorieul, Karin Pernet-Gallay, Caroline Marie-Desvergne, Iseult Lynch, Nathalie Herlin-Boime, and Marie Carriere
- Abstract
Titanium dioxide nanoparticles (TiO2-NPs) are one of the most produced NPs in the world. Their toxicity has been studied for a decade using acute exposure scenarios, i.e. high exposure concentrations and short exposure times. In the present study, we evaluated their genotoxic impact using long-term and low concentration exposure conditions. A549 alveolar epithelial cells were continuously exposed to 1–50 μg/mL TiO2-NPs, 86% anatase/14% rutile, 24 ± 6 nm average primary diameter, for up to two months. Their cytotoxicity, oxidative potential and intracellular accumulation were evaluated using MTT assay and reactive oxygen species measurement, transmission electron microscopy observation, micro-particle-induced X-ray emission and inductively-coupled plasma mass spectroscopy. Genotoxic impact was assessed using alkaline and Fpg-modified comet assay, immunostaining of 53BP1 foci and the cytokinesis-blocked micronucleus assay. Finally, we evaluated the impact of a subsequent exposure of these cells to the alkylating agent methyl methanesulfonate. We demonstrate that long-term exposure to TiO2-NPs does not affect cell viability but causes DNA damage, particularly oxidative damage to DNA and increased 53BP1 foci counts, correlated with increased intracellular accumulation of NPs. In addition, exposure over 2 months causes cellular responses suggestive of adaptation, characterized by decreased proliferation rate and stabilization of TiO2-NP intracellular accumulation, as well as sensitization to MMS. Taken together, these data underline the genotoxic impact and sensitization effect of long-term exposure of lung alveolar epithelial cells to low levels of TiO2-NPs.
- Published
- 2016
- Full Text
- View/download PDF
33. Diakonía. el servicio en la Biblia : Cuaderno bíblico 159
- Author
-
Paul Agneray, Jean-françois Baudoz, Jean-marie Carrière, Roselyne Dupont-roc, Marie-thérèse Perrot, Christophe Raimbault, Paul Agneray, Jean-françois Baudoz, Jean-marie Carrière, Roselyne Dupont-roc, Marie-thérèse Perrot, and Christophe Raimbault
- Abstract
La palabra «diaconía» no es corriente en español. No obstante, tiende a caracterizar una dimensión esencial de la vida cristiana subrayada actualmente por nuestras Iglesias. Una primera parte del presente Cuaderno (capítulos 1 a 3) amplía las necesarias definiciones de las palabras para una reflexión sobre las consecuencias concretas de la doble salvación narrada por las Escrituras: el fin de la servidumbre en Egipto según la Torá, por una parte; y el don de la vida en Cristo según Pablo, por otra. La segunda parte (capítulos 4 a 7) invita a leer cuatro textos fundamentales, de géneros muy diferentes, donde brilla la figura de Cristo servidor: 2 Corintios 8-9, Marcos 10,35-45, Hechos 6,1-7 y Juan 13,1-30.
- Published
- 2013
34. Médée protéiforme
- Author
-
Marie Carrière and Marie Carrière
- Subjects
- Fiction--Women authors--History and criticism
- Abstract
Le mythe de l'infanticide Médée a toujours connu une fortune littéraire et la littérature féminine contemporaine ne fait pas exception. L'analyse comparée de huit textes de femmes de divers horizons tente de cerner les enjeux de cette figure irréductible pour une pensée féministe actuelle sur la maternité, le sujet et l'écriture mythique. En s'interrogeant sur la pertinence particulière de la tragédie d'Euripide aux reprises médéennes, explicites ou sous-entendues, des femmes, cette étude comparée se penche sur des textes du théâtre de Marie Cardinal, Deborah Porter, Franca Rame et Cherríe Moraga, et des romans de Monique Bosco, Christa Wolf, Bessora et Marie-Célie Agnant. À travers ses incarnations transculturelles, le mythe de Médée éclaire les affres de l'exil et de l'exclusion, ainsi que certaines visions du maternel qui préféreraient peut-être rester dans l'ombre de nos présuppositions et de nos règles sociales. Bien qu'il n'y ait pas plus monstrueux ou fou que l'acte infanticide, Médée, elle, n'est pas monstre, pas folle, mais lucide, humaine à part entière, comme la voulait Euripide, alors qu'elle s'en prend à ses enfants, à la culture défectueuse, à l'histoire des hommes. La réécriture au féminin de Médée force aussi une conception du sujet qui ne revêt pas facilement sa cohérence. Mais la poétique même de cette Médée retranscrite au féminin fait preuve de sa flexibilité, son indétermination, son pouvoir de transcender la simple répétition de son mythe, vu ici autrement et différemment.
- Published
- 2012
35. Pre-validation of a reporter gene assay for oxidative stress for the rapid screening of nanobiomaterials
- Author
-
Sebastin Martin, Laura de Haan, Ignacio Miro Estruch, Kai Moritz Eder, Anne Marzi, Jürgen Schnekenburger, Magda Blosi, Anna Costa, Giulia Antonello, Enrico Bergamaschi, Chiara Riganti, David Beal, Marie Carrière, Olivier Taché, Gary Hutchison, Eva Malone, Lesley Young, Luisa Campagnolo, Fabio La Civita, Antonio Pietroiusti, Stéphanie Devineau, Armelle Baeza, Sonja Boland, Cai Zong, Gaku Ichihara, Bengt Fadeel, and Hans Bouwmeester
- Subjects
Nrf2 ,nanomaterial ,interlaboratory validation ,oxidative stress ,nanotoxicology ,Toxicology. Poisons ,RA1190-1270 - Abstract
Engineered nanomaterials have been found to induce oxidative stress. Cellular oxidative stress, in turn, can result in the induction of antioxidant and detoxification enzymes which are controlled by the nuclear erythroid 2-related factor 2 (NRF2) transcription factor. Here, we present the results of a pre-validation study which was conducted within the frame of BIORIMA (“biomaterial risk management”) an EU-funded research and innovation project. For this we used an NRF2 specific chemically activated luciferase expression reporter gene assay derived from the human U2OS osteosarcoma cell line to screen for the induction of the NRF2 mediated gene expression following exposure to biomedically relevant nanobiomaterials. Specifically, we investigated Fe3O4-PEG-PLGA nanomaterials while Ag and TiO2 “benchmark” nanomaterials from the Joint Research Center were used as reference materials. The viability of the cells was determined by using the Alamar blue assay. We performed an interlaboratory study involving seven different laboratories to assess the applicability of the NRF2 reporter gene assay for the screening of nanobiomaterials. The latter work was preceded by online tutorials to ensure that the procedures were harmonized across the different participating laboratories. Fe3O4-PEG-PLGA nanomaterials were found to induce very limited NRF2 mediated gene expression, whereas exposure to Ag nanomaterials induced NRF2 mediated gene expression. TiO2 nanomaterials did not induce NRF2 mediated gene expression. The variability in the results obtained by the participating laboratories was small with mean intra-laboratory standard deviation of 0.16 and mean inter laboratory standard deviation of 0.28 across all NRF2 reporter gene assay results. We conclude that the NRF2 reporter gene assay is a suitable assay for the screening of nanobiomaterial-induced oxidative stress responses.
- Published
- 2022
- Full Text
- View/download PDF
36. Nanoparticules d’or
- Author
-
Olivier PLUCHERY and Marie CARRIERE
- Published
- 2011
37. Immediate and Sustained Effects of Cobalt and Zinc-Containing Pigments on Macrophages
- Author
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Julie Devcic, Manon Dussol, Véronique Collin-Faure, Julien Pérard, Daphna Fenel, Guy Schoehn, Marie Carrière, Thierry Rabilloud, and Bastien Dalzon
- Subjects
macrophages ,cobalt-based pigment ,zinc-based pigment ,persisting effects ,phagocytosis ,inflammation ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Pigments are among the oldest nanoparticulate products known to mankind, and their use in tattoos is also very old. Nowadays, 25% of American people aged 18 to 50 are tattooed, which poses the question of the delayed effects of tattoos. In this article, we investigated three cobalt [Pigment Violet 14 (purple color)] or cobalt alloy pigments [Pigment Blue 28 (blue color), Pigment Green 14 (green color)], and one zinc pigment [Pigment White 4 (white color)] which constitute a wide range of colors found in tattoos. These pigments contain microparticles and a significant proportion of submicroparticles or nanoparticles (in either aggregate or free form). Because of the key role of macrophages in the scavenging of particulate materials, we tested the effects of cobalt- and zinc-based pigments on the J774A.1 macrophage cell line. In order to detect delayed effects, we compared two exposure schemes: acute exposure for 24 hours and an exposure for 24 hours followed by a 3-day post-exposure recovery period. The conjunction of these two schemes allowed for the investigation of the delayed or sustained effects of pigments. All pigments induced functional effects on macrophages, most of which were pigment-dependent. For example, Pigment Green 19, Pigment Blue 28, and Pigment White 4 showed a delayed alteration of the phagocytic capacity of cells. Moreover, all the pigments tested induced a slight but significant increase in tumor necrosis factor secretion. This effect, however, was transitory. Conversely, only Pigment Blue 28 induced both a short and sustained increase in interleukin 6 secretion. Results showed that in response to bacterial stimuli (LPS), the secretion of tumor necrosis factor and interleukin 6 declined after exposure to pigments followed by a recovery period. For chemoattractant cytokines (MCP-1 or MIP-1α), delayed effects were observed with a secretion decreased in presence of Pigment Blue 28 and Pigment violet 14, both with or without LPS stimuli. The pigments also induced persisting changes in some important macrophage membrane markers such as CD11b, an integrin contributing to cell adhesion and immunological tolerance. In conclusion, the pigments induced functional disorders in macrophages, which, in some cases, persist long after exposure, even at non-toxic doses.
- Published
- 2022
- Full Text
- View/download PDF
38. Preliminary Toxicological Analysis in a Safe-by-Design and Adverse Outcome Pathway-Driven Approach on Different Silver Nanoparticles: Assessment of Acute Responses in A549 Cells
- Author
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Giulia Motta, Maurizio Gualtieri, Melissa Saibene, Rossella Bengalli, Andrea Brigliadori, Marie Carrière, and Paride Mantecca
- Subjects
nano-enabled products ,adverse outcomes pathway ,safe-by-design ,in vitro lung cells ,nanotoxicity ,silver nanoparticle hazard ,Chemical technology ,TP1-1185 - Abstract
Silver nanoparticles (Ag NPs) are among the most widely used metal-based nanomaterials (NMs) and their applications in different products, also as antibacterial additives, are increasing. In the present manuscript, according to an adverse outcome pathway (AOP) approach, we tested two safe-by-design (SbD) newly developed Ag NPs coated with hydroxyethyl cellulose (HEC), namely AgHEC powder and AgHEC solution. These novel Ag NPs were compared to two reference Ag NPs (naked and coated with polyvinylpyrrolidone—PVP). Cell viability, inflammatory response, reactive oxygen species, oxidative DNA damage, cell cycle, and cell–particle interactions were analyzed in the alveolar in vitro model, A549 cells. The results show a different toxicity pattern of the novel Ag NPs compared to reference NPs and that between the two novel NPs, the AgHEC solution is the one with the lower toxicity and to be further developed within the SbD framework.
- Published
- 2023
- Full Text
- View/download PDF
39. 6. Tracing the (M)Other: Erin Mouré
- Author
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Marie Carriere
- Subjects
media_common.quotation_subject ,Art history ,Art ,Tracing ,media_common - Published
- 2002
40. 4. Questioning the Mother: Di Brandt
- Author
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Marie Carriere
- Subjects
Anthropology ,Gender studies ,Biology - Published
- 2002
41. 7. Mothering Text: Lola Lemire Tostevin
- Author
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Marie Carriere
- Published
- 2002
42. 3. Resurrecting the (M)Other: Nicole Brossard
- Author
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Marie Carriere
- Published
- 2002
43. 9. An Ethics of Love: Brandt, Mouré, and Brossard
- Author
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Marie Carriere
- Subjects
Psychoanalysis ,Philosophy ,Humanities - Published
- 2002
44. 5. Performing Hysteria: France Théoret
- Author
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Marie Carriere
- Subjects
Psychoanalysis ,medicine ,Hysteria ,Psychology ,medicine.disease - Published
- 2002
45. 1. Introduction to Writing in the Feminine
- Author
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Marie Carriere
- Published
- 2002
46. 8. An Ethics of Selfhood: Théoret and Tostevin
- Author
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Marie Carriere
- Subjects
Aesthetics ,Philosophy ,Art history - Published
- 2002
47. 2. Writing (As) a Feminist Ethics
- Author
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Marie Carriere
- Subjects
Political science ,Gender studies ,Feminist philosophy ,Feminist ethics - Published
- 2002
48. Writing in the Feminine in French and English Canada
- Author
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Marie Carriere
- Published
- 2002
49. The State of the Art and Challenges of In Vitro Methods for Human Hazard Assessment of Nanomaterials in the Context of Safe-by-Design
- Author
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Nienke Ruijter, Lya G. Soeteman-Hernández, Marie Carrière, Matthew Boyles, Polly McLean, Julia Catalán, Alberto Katsumiti, Joan Cabellos, Camilla Delpivo, Araceli Sánchez Jiménez, Ana Candalija, Isabel Rodríguez-Llopis, Socorro Vázquez-Campos, Flemming R. Cassee, and Hedwig Braakhuis
- Subjects
nanomaterials ,safe-by-design ,hazard testing ,in vitro methods ,SAbyNA ,advanced materials ,Chemistry ,QD1-999 - Abstract
The Safe-by-Design (SbD) concept aims to facilitate the development of safer materials/products, safer production, and safer use and end-of-life by performing timely SbD interventions to reduce hazard, exposure, or both. Early hazard screening is a crucial first step in this process. In this review, for the first time, commonly used in vitro assays are evaluated for their suitability for SbD hazard testing of nanomaterials (NMs). The goal of SbD hazard testing is identifying hazard warnings in the early stages of innovation. For this purpose, assays should be simple, cost-effective, predictive, robust, and compatible. For several toxicological endpoints, there are indications that commonly used in vitro assays are able to predict hazard warnings. In addition to the evaluation of assays, this review provides insights into the effects of the choice of cell type, exposure and dispersion protocol, and the (in)accurate determination of dose delivered to cells on predictivity. Furthermore, compatibility of assays with challenging advanced materials and NMs released from nano-enabled products (NEPs) during the lifecycle is assessed, as these aspects are crucial for SbD hazard testing. To conclude, hazard screening of NMs is complex and joint efforts between innovators, scientists, and regulators are needed to further improve SbD hazard testing.
- Published
- 2023
- Full Text
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50. Physico-Chemical Transformation and Toxicity of Multi-Shell InP Quantum Dots under Simulated Sunlight Irradiation, in an Environmentally Realistic Scenario
- Author
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Fanny Dussert, Géraldine Sarret, Karl David Wegner, Olivier Proux, Gautier Landrot, Pierre-Henry Jouneau, Peter Reiss, and Marie Carrière
- Subjects
quantum dot ,indium ,safer-by-design ,environmental degradation ,toxicity ,EXAFS ,Chemistry ,QD1-999 - Abstract
Quantum dots (QDs) are widely used in optoelectronics, lighting, and photovoltaics leading to their potential release into the environment. The most promising alternative to the highly toxic cadmium selenide (CdSe) QDs are indium phosphide (InP) QDs, which show reduced toxicity and comparable optical and electronic properties. QD degradation leads to the release of toxic metal ions into the environment. Coating the QD core with robust shell(s) composed of another semi-conductor material enhances their properties and protects the QD from degradation. We recently developed double-shelled InP QDs, which proved to be less toxic than single-shell QDs. In the present study, we confirm their reduced cytotoxicity, with an LC50 at 77 nM for pristine gradient shell QDs and >100 nM for pristine thin and thick shell QDs. We also confirm that these three QDs, when exposed to simulated sunlight, show greater cytotoxicity compared to pristine ones, with LC50 ranging from 15 to 23 nM. Using a combination of spectroscopic and microscopic techniques, we characterize the degradation kinetics and transformation products of single- and double-shell QDs, when exposed to solar light at high temperature, simulating environmental conditions. Non-toxic pristine QDs degrade to form toxic In–phosphate, In–carboxylate, Zn–phosphate, and oxidized Se, all of which precipitate as heterogeneous deposits. Comparison of their degradation kinetics highlights that the QDs bearing the thickest ZnS outer shell are, as expected, the most resistant to photodegradation among the three tested QDs, as gradient shell, thin shell, and thick shell QDs lose their optical properties in less than 15 min, 60 min, and more than 90 min, respectively. They exhibit the highest photoluminescence efficiency, i.e., the best functionality, with a photoluminescence quantum yield in aqueous solution of 24%, as compared to 18% for the gradient shell and thin shell QDs. Therefore, they can be considered as safer-by-design QDs.
- Published
- 2022
- Full Text
- View/download PDF
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