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O objetivo deste estudo foi obter o perfil eletroforetico das proteinas sericas em eguas ciclicas e verificar as diferencas entre as fases folicular e luteal do ciclo estral nesta especie. Foram utilizadas 18 eguas, totalizando 36 amostras de soro, sendo duas de cada egua. As amostras foram colhidas no estro e no diestro. As proteinas sericas totais foram obtidas pelo metodo do Biureto, a partir da utilizacao de Kits comerciais (LABTEST) e, as diferentes subfracoes proteicas, por eletroforese em gel de poliacrilamida (SDS-PAGE). O eletroforetograma das proteinas sericas colocou em evidencia a presenca de 17 a 25 fracoes proteicas, cujos pesos moleculares variaram de 22 a 254 kDa. Identificaram-se duas proteinas ainda nao nomeadas oficialmente, de massas moleculares (MM) 23 kDa e 144 kDa. Os valores medios ± SEM obtidos para cada variavel no estro e no diestro, respectivamente, foram: proteinas totais (g/dL) 7,11 ± 0,07 e 7,36 ± 0,07; albumina (mg/dL) 4790,83 ± 69,10 e 5027,19 ± 69,10; α1 glicoproteina acida (mg/dL) 4,90 ± 0,31 e 4,93 ± 0,31; ceruloplasmina (mg/dL) 15,28 ± 1,31 e 10,65 ± 1,31; haptoglobina (mg/dL) 22,70 ± 1,16 e 27,06 ± 1,16; transferrina (mg/dL) 329,00 ± 9,78 e 350,16 ± 9,78; IgA (mg/dL) 119,91 ± 6,30 e 107,03 ± 6,30; IgG (mg/dL) 1525,07 ± 40,18 e 1517,25 ± 40,18; MM 23 (mg/dL) 204,44 ± 8,61 e 219,79 ± 8,61; MM 144 (mg/dL) 22,13 ± 0,55 e 21,49 ± 0,55. Nao houve diferenca significativa das proteinas totais e suas fracoes do estro para o diestro. Conclui-se que as modificacoes hormonais durante as fases do ciclo estral da egua nao interferem no proteinograma serico.

Made available in DSpace on 2019-10-06T16:43:12Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-12-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Canine congenital malformations are structural or functional abnormalities of organs present at birth that possibly interfere with the viability of newborns, thus contributing to neonatal mortality. This study evaluated and described the incidence of congenital malformations in neonatal dogs and determined the mortality rates among those affected. Of the 178 litters and 803 newborns included in the study, 24.7% (44/178) of the litters presented neonates with congenital malformations. The total rate of neonates that presented malformations was 6.7% (64/803). The total mortality rate in newborns with congenital defects was 5.4% (44/803), representing 68.7% (44/64) of the deaths observed among those affected. The early (0–2 days old) and late (3–30 days old) mortality rates among the affected neonates were 61.4% (27/44) and 38.6% (17/44), respectively. In total, 27 malformations were recorded, and the most common congenital defects were cleft palate 2.8% (23/803) and hydrocephaly 1.5% (12/803), either alone or associated with other malformations. The malformations were recorded in 15 breeds: Pug, Miniature Pinscher, Rottweiler, Pitbull, French Bulldog, English Bulldog, Dachshund, Labrador Retriever, Lhasa Apso, Poodle, German Spitz, Yorkshire Terrier, Shih-tzu, Brazilian Terrier and mixed breed. One case of exposure to a teratogenic agent was reported, but no maternal exposure to teratogens during gestation was reported with the other litters. The occurrence of congenital defects may be related to genetic factors since the highest incidence of malformations (84.4%) was observed in purebred dogs. Department of Veterinary Clinics School of Veterinary Medicine and Animal Sciences São Paulo State University (UNESP) Department of Animal Breeding and Nutrition School of Veterinary Medicine and Animal Sciences São Paulo State University (UNESP) Department of Veterinary Anesthesiology and Surgery School of Veterinary Medicine and Animal Sciences São Paulo State University (UNESP) Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Sciences São Paulo State University (UNESP) Department of Veterinary Clinics School of Veterinary Medicine and Animal Sciences São Paulo State University (UNESP) Department of Animal Breeding and Nutrition School of Veterinary Medicine and Animal Sciences São Paulo State University (UNESP) Department of Veterinary Anesthesiology and Surgery School of Veterinary Medicine and Animal Sciences São Paulo State University (UNESP) Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Sciences São Paulo State University (UNESP)