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Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Hospital Ophir Loyola. Departamento de Patologia. Bel?m, Par?, Brasil. Centro Universit?rio do Estado do Par?. Bel?m, Par?, Brasil. Universidade Federal do Par?. N?cleo de Medicina Tropical. Bel?m, Par?, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Os linfomas s?o neoplasias do sistema imunit?rio com origem em linf?citos B, T ou c?lulas natural killer que envolvem os tecidos linfoides que est?o associados ao crescimento de massas tumorais. O linfoma de Hodgkin (LH) ? uma neoplasia linfoproliferativa caracterizada histopatologicamente pela presen?a de c?lulas neopl?sicas com variada morfologia denominadas Reed-Sternberg. O objetivo deste trabalho foi descrever o perfil epidemiol?gico e subtipos dos LH diagnosticados no per?odo de 1996 a 2005 no Hospital Ophir Loyola em Bel?m, Estado do Par?, Brasil. Do total, 64,6 % (42/65) dos pacientes com LH eram do sexo masculino e 35,4 % (23/65) do feminino com faixa et?ria variando entre 2 e 84 anos. Os subtipos de LH registraram taxas de 50,8 % (33/65), 26,2% (17/65), 15,4% (10/65) e 7,6% (5/65) para esclerose nodular, celularidade mista, deple??o linfocit?ria e predomin?ncia linfocit?ria, respectivamente. Os LH, na d?cada do estudo (1996-2005), foram mais expressivos na faixa infanto-juvenil, na qual prevaleceu o subtipo esclerose nodular nas amostras diagnosticadas no Estado do Par?. Lymphomas are neoplasias of the immune system originating from B and T cells or natural killer cells which involve lymphoid tissues that are associated with the growth of tumor masses. Hodgkin's lymphoma (HL) is a lymphoproliferative malignancy characterized pathologically by the presence of neoplastic cells with a different morphology called Reed-Sternberg. The aim of this study was to describe the epidemiological profile and subtypes of HL diagnosed from 1996 to 2005 at Ophir Loyola Hospital in Bel?m, Par? State, Brazil. Of the total, 64.6% (42/65) of patients with HL were male and 35.4 % (23/65) of women with ages ranging from 2 to 84 years old. HL subtypes reported rates of 50.8 % (33/65), 26.2% (17/65), 15.4 % (10/65) and 7.6% (5/65) to nodular sclerosis, mixed cellularity, lymphocyte depletion and lymphocyte predominance, respectively. HL, in the period of the current study (1996-2005), it was more expressive in groups of children and teenagers which prevailed the nodular sclerosis subtype in samples diagnosed in the Par? State.

Adenoid cystic carcinoma (ACC) is an intriguing lesion because it shows a slow growth in the beginning, but a late poor prognosis due to perineural invasion, metastasis and recurrence. This study aimed to investigate whether Akt signaling would be deregulated in adenoid cystic carcinoma and its consequence in the expression of associated proteins. The expression of the Akt, p-Akt, NFκB, β-catenin, cyclin D1 and COX-2 was assessed by immunohistochemistry in 10 cases of ACC, 17 cases of pleomorphic adenoma (PA), and 7 cases of normal salivary gland (NSG). p-Akt was overexpressed in ACC when compared to NSG. NFκB, β-catenin, and COX-2 were overexpressed in ACC and PA when compared to NSG. Most proteins were slightly higher expressed in ACC than in PA, but they never reached significance. p-Akt expression positively correlated with NFκB, β-catenin, cyclin D1 and COX-2 in ACC and PA, while this correlation trended to be negative in for these proteins (except for NFκB) in NSG using Person’s correlation analysis, but without reaching significance. Our results indicate an abnormal activation of Akt signaling pathway, which can be an important regulator of tumor biology in ACC. Activated Akt correlated with the expression of NFκB, β-catenin and COX-2, which can potentially influence cell survival in ACC.

Squamous cell carcinoma is a prevalent head and neck tumor with high mortality. We studied the role played by laminin alpha1 chain peptide AG73 on migration, invasion, and protease activity of cells (OSCC) from human oral squamous cell carcinoma. Immunohistochemistry and immunofluorescence analyzed expression of laminin alpha1 chain and MMP9 in oral squamous cells carcinoma in vivo and in vitro. Migratory activity of AG73-treated OSCC cells was investigated by monolayer wound assays and in chemotaxis chambers. AG73-induced invasion was assessed in Boyden chambers. Invasion depends on MMPs. Conditioned media from cells grown on AG73 was subjected to zymography. We searched for AG73 receptors related to these activities in OSCC cells. Immunofluorescence analyzed AG73-induced colocalization of syndecan-1 and beta1 integrin. Cells had these receptors silenced by siRNA, followed by treatment with AG73 and analysis of migration, invasion, and protease activity. Oral squamous cell carcinoma expresses laminin alpha1 chain and MMP9. OSCC cells treated with AG73 showed increased migration, invasion, and protease activity. AG73 induced colocalization of syndecan-1 and beta1 integrin. Knockdown of these receptors decreased AG73-dependent migration, invasion, and protease activity. Syndecan-1 and beta1 integrin signaling downstream of AG73 regulate migration, invasion, and MMP production by OSCC cells.

São descritos três casos de feo-hifomicose subcutânea, os primeiros encontrados no Estado do Pará, todos oriundos de localidades situadas a leste de Belém, nas micron-regiões do Salgado e Bragantina. O diagnóstico teve por base o reconhecimento dos parasitos em cortes histológicos, sem identificação de espécie, uma vez que todo o material disponível para exame - compreendendo as lesões completamente excisadas -, ao ser recebido, já estava fixado em formol e imprestável, portanto, para qualquer tentativa de estudo micológico. A abmidância de elementos fúngicos verificada nos tecidos, após impregnação pela prata (métodos de Grocott e de Fontana-Masson), contrastava com a dificuldade para visualizá-los em cortes não-corados ou corados por hematoxilina-eosina, fato devido certamente ao aspecto das hifas neles existentes: delgadas, de paredes finas e cor quase imperceptível.Three cases of subcutaneous phaeohyphomycosis from the State of Para, Brazil, are reported. This is the first time that this form of phaeohyphomycosis is described in Northern Brazil. The etiologic agents could not be isolated, and the diagnosis in all cases was stablished only on histopathological basis, particularly through the demonstration of fungal elements in tissue sections. Hyphal fragments and vesicidar cells were very numerous and conspicuous in special (Grocott and Fontana-Masson methods) stained sections, in contrast to their apparent scarcity in routine hematoxilin-eosin preparations. The difference was certainly due to the aspect in the latter of the invasive tissue forms: the thin-walled and light brown pigmented organisms stained poorly and were veiy difficult to distinguish from the tissue components.

São descritos dois casos de zigomicose nasofacial, procedentes de Belém, PA. O achado indica não ser tão rara, como se poderia supor, pela falta de registros, a ocorrência dessa micose na Região Norte do Brasil. Em ambos os pacientes, o tratamento pelo cetoconazol determinou involução rápida das manifestações clínicas, principalmente a obstrução nasal. Com o presente relato, sobe para cinco o número de casos da infecção referidos até agora do Estado do Pará.

Hospital Adventista de Bel?m. Bel?m, PA, Brasil. Minist?rio da Sa?de. Funda??o Nacional de Sa?de. Instituto Evandro Chagas. Bel?m, PA, Brasil. Universidade de Bras?lia. Bras?lia, D F, Brasil.