6 results on '"Maria S. Romero"'
Search Results
2. Acceptance of a Pre-visit Intervention to Engage African American Glaucoma Patients during Visits
- Author
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Betsy Sleath, Delesha M. Carpenter, Scott A. Davis, Donald L. Budenz, Kelly W. Muir, Maria S. Romero, Charles Lee, Gail Tudor, Nacire Garcia, Abena A. Adjei, and Alan L. Robin
- Subjects
Ophthalmology ,Optometry - Abstract
The glaucoma question prompt list/video intervention was well received by patients. Eighty-seven percent of patients recommended that other patients should watch the educational video before their visits, and 89% said that other patients should complete the question prompt list before visits.The objectives of this study were to (a) describe patient feedback on a glaucoma question prompt list/video intervention designed to motivate African American patients to be more engaged during visits and (b) examine patient demographics associated with acceptance of the intervention.We are conducting a randomized controlled trial of a glaucoma question prompt list/video intervention. African American patients with glaucoma were enrolled and assigned to a control group or an intervention group where they watched a video emphasizing the importance of asking questions and received a prompt list to complete before visits. All patients were interviewed after visits and are being followed up for 12 months.One hundred eighty-nine African American patients with glaucoma were enrolled into the larger trial. Of the 93 patients randomized to the intervention group, 89% said that patients should complete the prompt lists before visits, and 87% recommended that patients should watch the video before visits. Older patients were significantly less likely to believe that other patients should watch the video before their visits (t = -3.7, P = .04). Patients with fewer years of education were significantly more likely to rate the video as being more useful than patients with more years of education (Pearson correlation, -0.27; P = .01). Patients who reported being less adherent on the visual analog scale were more likely to rate the video as being more useful (Pearson correlation, -0.23; P = .03).This study demonstrates that the question prompt list/video was accepted by the majority of African American patients who received the intervention.
- Published
- 2023
3. Provider use of a participatory decision-making style with African American patients with glaucoma
- Author
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Betsy Sleath, Delesha M. Carpenter, Donald L. Budenz, Kelly W. Muir, Maria S. Romero, Gail Tudor, Nacire Garcia, Abena A. Adjei, and Alan L. Robin
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General Medicine - Published
- 2023
- Full Text
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4. Characterization of Citrus sinensis transcription factors closely associated with the non-host response to Xanthomonas campestris pv. vesicatoria
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Francisco R. Tadeo, Manuel Talon, Elena G. Orellano, Lucas D. Daurelio, Maria S. Romero, Adriana Cortadi, Paz Merelo, and Silvana Petrocelli
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Hypersensitive response ,Microarray transcriptome analysis ,Physiology ,Plant Science ,Genes, Plant ,Xanthomonas campestris ,Xanthomonas campestris pv. vesicatoria ,Non-host response ,Xanthomonas citri ,Ciencias Biológicas ,Gene Expression Regulation, Plant ,Stress, Physiological ,Botany ,Alleles ,Plant Proteins ,Expressed Sequence Tags ,Genetics ,Cell Death ,biology ,Gene Expression Profiling ,fungi ,food and beverages ,Xanthomonas campestris pv. Vesicatoria ,Bioquímica y Biología Molecular ,Biotic stress ,biology.organism_classification ,WRKY protein domain ,Plant Leaves ,Host-Pathogen Interactions ,Citrus canker ,Xanthomonas citri subsp. citri ,Agronomy and Crop Science ,CIENCIAS NATURALES Y EXACTAS ,Citrus × sinensis ,Citrus sinensis ,Transcription Factors - Abstract
Summary Plants, when exposed to certain pathogens, may display a form of genotype-independent resistance, known as non-host response. In this study, the response of Citrus sinensis (sweet orange) leaves to Xanthomonas campestris pv. vesicatoria (Xcv), a pepper and tomato pathogenic bacterium, was analyzed through biochemical assays and cDNA microarray hybridization and compared with Asiatic citrus canker infection caused by Xanthomonas citri subsp. citri. Citrus leaves exposed to the non-host bacterium Xcv showed hypersensitive response (HR) symptoms (cell death), a defense mechanism common in plants but poorly understood in citrus. The HR response was accompanied by differentially expressed genes that are associated with biotic stress and cell death. Moreover, 58 transcription factors (TFs) were differentially regulated by Xcv in citrus leaves, including 26 TFs from the stress-associated families AP2-EREBP, bZip, Myb and WRKY. Remarkably, in silico analysis of the distribution of expressed sequence tags revealed that 10 of the 58 TFs, belonging to C2C2-GATA, C2H2, CCAAT, HSF, NAC and WRKY gene families, were specifically over-represented in citrus stress cDNA libraries. This study identified candidate TF genes for the regulation of key steps during the citrus non-host HR. Furthermore, these TFs might be useful in future strategies of molecular breeding for citrus disease resistance. Fil: Daurelio, Lucas Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Romero, Maria S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Petrocelli, Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Merelo, Paz. Institut Valencià d’Investigacions Agràries. Centre de Genómica; España Fil: Cortadi, Adriana Amalia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Talon, Manuel. Institut Valencià d’Investigacions Agràries. Centre de Genómica; España Fil: Tadeo, Francisco R.. Institut Valencià d’Investigacions Agràries. Centre de Genómica; España Fil: Orellano, Elena Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
- Published
- 2013
- Full Text
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5. December consultation #6
- Author
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Maria S. Romero
- Subjects
Ophthalmology ,Surgery ,Sensory Systems - Published
- 2018
- Full Text
- View/download PDF
6. Chemical Synthesis and Functional Analysis of VarvA Cyclotide
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Claudio A. Álvarez, Paula A. Santana, Omar Luna, Constanza Cárdenas, Fernando Albericio, María S. Romero, and Fanny Guzmán
- Subjects
cyclotide ,antimicrobial activity ,fish pathogens ,membrane damage ,Organic chemistry ,QD241-441 - Abstract
Cyclotides are circular peptides found in various plant families. A cyclized backbone, together with multiple disulfide bonds, confers the peptides’ exceptional stability against protease digestion and thermal denaturation. In addition, the features of these antimicrobial molecules make them suitable for use in animal farming, such as aquaculture. Fmoc solid phase peptide synthesis on 2-chlorotrityl chlorine (CTC) resin using the “tea-bag” approach was conducted to generate the VarvA cyclotide identified previously from Viola arvensis. MALDI-TOF mass spectrometry determined the correct peptide amino acid sequence and the cyclization sites-critical in this multicyclic compound. The cyclotide showed antimicrobial activity against various Gram-negative bacteria, including recurrent pathogens present in Chilean aquaculture. The highest antimicrobial activity was found to be against Flavobacterium psychrophilum. In addition, membrane blebbing on the bacterial surface after exposure to the cyclotide was visualized by SEM microscopy and the Sytox Green permeabilization assay showed the ability to disrupt the bacterial membrane. We postulate that this compound can be proposed for the control of fish farming infections.
- Published
- 2018
- Full Text
- View/download PDF
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