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Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:50:52Z No. of bitstreams: 1 S0100-736X2010000100013.pdf: 226146 bytes, checksum: 442aa8a106b64ca56d9a8dcf97eb707b (MD5) Made available in DSpace on 2013-08-22T18:50:52Z (GMT). No. of bitstreams: 1 S0100-736X2010000100013.pdf: 226146 bytes, checksum: 442aa8a106b64ca56d9a8dcf97eb707b (MD5) Previous issue date: 2010-01-01 Made available in DSpace on 2013-09-30T18:24:13Z (GMT). No. of bitstreams: 2 S0100-736X2010000100013.pdf: 226146 bytes, checksum: 442aa8a106b64ca56d9a8dcf97eb707b (MD5) S0100-736X2010000100013.pdf.txt: 42445 bytes, checksum: df949829011c8a2dbd82f1df033e19e9 (MD5) Previous issue date: 2010-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:33:10Z No. of bitstreams: 2 S0100-736X2010000100013.pdf: 226146 bytes, checksum: 442aa8a106b64ca56d9a8dcf97eb707b (MD5) S0100-736X2010000100013.pdf.txt: 42445 bytes, checksum: df949829011c8a2dbd82f1df033e19e9 (MD5) Made available in DSpace on 2014-05-20T13:33:10Z (GMT). No. of bitstreams: 2 S0100-736X2010000100013.pdf: 226146 bytes, checksum: 442aa8a106b64ca56d9a8dcf97eb707b (MD5) S0100-736X2010000100013.pdf.txt: 42445 bytes, checksum: df949829011c8a2dbd82f1df033e19e9 (MD5) Previous issue date: 2010-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) O objetivo do trabalho foi avaliar o perfil eletroforético das proteínas e a concentração sérica de imunoglobulina G (IgG) em cabras da raça Saanen com mastite induzida experimentalmente por Staphylococcus aureus e suplementadas com vitamina E (acetato de dl-α-tocoferol). Utilizaram-se 14 cabras adultas, gestantes, primíparas, com sorologia negativa para Artrite Encefalite Caprina (CAEV), clinicamente sadias, divididas em dois grupos experimentais de sete animais. Grupo não suplementado (G1) e grupo suplementado com 2.000 U.I. de acetato de dl-α-tocoferol (G2 Vit E) via intramuscular no dia do parto e sete dias após o parto. Ao nono dia do pós-parto foram inoculados 300 UFCs da cepa de S. aureus ATCC 225923, na metade esquerda da glândula mamária de cada animal. A mastite foi determinada pela colheita das amostras de leite para a comprovação da infecção, por meio de exames bacteriológicos, contagem de células somáticas (CCS) e California Mastitis Test (CMT), a partir deste momento foram efetuadas colheitas às 12, 24, 48 e 72 horas, sendo posteriormente instituído o tratamento intramamário com antimicrobiano e nova avaliação 48 horas após o tratamento. O perfil eletroforético em gel de agarose das proteínas séricas das cabras, apresentaram cinco frações, sendo: albumina e globulinas (α, β1, β2 e γ). Houve aumento na produção de γ-globulina e menor produção da fração β2-globulina 12 horas após a infecção, com os valores reduzindo mais rapidamente no grupo suplementado, evidenciando a influência da vitamina E na diminuição da produção das proteínas de fase aguda. Não houve influência da vitamina E na concentração sérica de imunoglobulina G (IgG) nos animais suplementados. A suplementação com vitamina E aumentou a concentração de imunoglobulinas e diminuiu a produção de proteínas de fase aguda, provavelmente pelo efeito antioxidante minimizando a lesão tecidual durante o processo inflamatório localizado na glândula mamária. The objective was to evaluate the electrophoretic profile of proteins and serum concentration of immunoglobulin G (IgG) in Saanen goats with mastitis experimentally induced by Staphylococcus aureus (dl-α-tocopherol acetated). 14 adult goats, (supplemented with vitamin E DL-α-tocopherol) primiparous pregnant, seronegative for caprine arthritis encephalitis (CAEV), clinically healthy, were divided into two groups of seven animals: Not supplemented group (G1) and group supplemented with 2.000 UI of DL-α-tocopherol (G2 Vit E), by intramuscular injection on the day of the parturition and seven days later. At the 9th day after delivery 300 UFCs of the S. aureus ATCC 225923 strain were inoculated into the left half of the mammary gland of each animal. The mastitis was determined through collection of milk samples for evidence of infection by means of bacteriological examination, somatic cell count (SCC) and California Mastitis Test (CMT). Then samples were collected after 12, 24, 48 and 72 hours, antimicrobial intra-mammary gland treatment was initiated, with new evaluation 48 hours after treatment. The electrophoretic profile of serum protein of the goats, showed five fractions, as follows: albumin and globulin (α, β1, β2 e γ-globulin). There was an increase in the production of γ-globulin and lower production of β2-globulin fraction 12 hours after infection, and faster decrease in the supplemented group, showing the influence of vitamin E in the production of acute phase proteins. There was no influence of vitamin E in the serum concentration of immunoglobulin G (IgG) in supplemented animals. The supplementation with vitamin E increased the concentration of immunoglobulin and decreased the production of acute phase proteins, probably the antioxidant effect minimizing the tissue injury during the inflammatory process in the mammary gland. Universidade do Estado de Santa Catarina (UDESC) Centro de Ciências Agroveterinárias Departamento de Medicina Veterinária Universidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Medicina Veterinária e Zootecnia Departamento de Clínica Médica Universidade Federal de Santa Maria (UFSM) Departamento de Clínica de Pequenos Animais Universidade Federal de Minas Gerais Departamento de Clínica e Cirurgia Veterinária Unesp Instituto de Biociências Departamento de Nutrição Unesp FMVZ Departamento de Higiene Sanitária e Saúde Pública Universidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Medicina Veterinária e Zootecnia Departamento de Clínica Médica Unesp Instituto de Biociências Departamento de Nutrição Unesp FMVZ Departamento de Higiene Sanitária e Saúde Pública

The renal prostaglandins (PGs), vasodilators, preserve kidney function during increased activity of the renin-angiotensin system or renal sympathetic nerves (renal PG-dependent state [RPGD]). Ketoprofen (Ket) inhibits cyclooxygenase and, therefore, the synthesis of PGs. The aim of this study was to determine, in the rat, the action of Ket in the renal histology and function in a RPGD state (stress of anesthesia and hemorrhage).Twenty male Wistar rats, anesthetized with sodium pentobarbital, were randomly divided into two groups: G1--control (n = 10) and G2-Ket (n = 10) submitted to arterial hemorrhage of 30% of volemia (estimated as 6% of body weight) three times (10% each 10 min), 65 min after anesthesia. G2 animals received Ket, 1.5 mg. kg(-1), venously, 5 min after anesthesia and 60 min before the first hemorrhage moment (first moment of the study [M1]). Medium arterial pressure (MAP), rectal temperature (T), and heart rate were monitored. G1 and G2 received para-aminohippurate sodium (PAH) and iothalamate sodium (IOT) solutions during the entire experimental time in order to determine clearance of PAH (effective renal plasma flow [ERPF]) and clearance of IOT (glomerular filtration rate [GFR]) without urine collection (determination of blood concentrations of PAH and IOT through the high-performance liquid chromatography), filtration fraction (FF), and renal vascular resistance (RVR). The animals were sacrificed in M3, 30 min after the third hemorrhage (M2) moment, and the kidneys and blood collected during the hemorrhage periods were utilized for histological study and determinations of hematocrit (Ht), serum creatinine (SCr), ERPF, GFR, FF, and RVR, respectively.There were significant reductions of MAP, T, and Ht and a significant increase of SCr. During the experiment, ERPF and GFR did not change, but ERPF was always higher in G1 than in G2. Ket did not alter FF, which increased in G1 over the duration of experiment. The Ket group had significantly higher RVR than the control group. The histology verified that both G1 and G2 were similar for tubular dilation and necrosis, but they were significantly different for tubular degeneration: G1G2.The changes observed in kidney histology probably were determined by hemorrhage and hypotension. Ket inhibited the synthesis of PGs and diminished tubular degeneration.

Objective To determine the influence of a low-dose constant rate infusion (LCRI; 50 μg kg −1 minute −1 ) and high-dose CRI (HCRI; 200 μg kg −1 minute −1 ) lidocaine infusion on the minimum alveolar concentration (MAC) of isoflurane (I) in dogs. Study design Prospective experimental study. Animals Ten mongrel dogs (four females, six males), weighing 20–26.3 kg. Methods Dogs were anesthetized with I in oxygen and their lungs mechanically ventilated. Baseline MAC was determined using mechanical or electrical stimuli. Lidocaine (2 mg kg −1 IV) was administered over 3 minutes, followed by the LCRI and MAC determination commenced 30 minutes later. Once MAC was determined following LCRI, the lidocaine infusion was stopped for 30 minutes. A second bolus of lidocaine (2 mg kg −1 , IV) was administered, followed by the HCRI and MAC re-determined. Concentrations of lidocaine and its metabolites were measured at end-tidal I concentrations immediately above and below MAC. Heart rates and blood pressures were measured. Results Minimum alveolar concentration of I was 1.34 ± 0.11 (%; mean ± SD) for both types of stimulus. The LCRI significantly reduced MAC to 1.09 ± 0.13 (18.7% reduction) and HCRI to 0.76 ± 0.10 (43.3% reduction). Plasma concentrations (ng mL −1 , median; value below and above MAC, respectively) for LCRI were: lidocaine, 1465 and 1537; glycinexylidide (GX), 111 and 181; monoethylglycinexylidide (MEGX), 180 and 471 and for HCRI were: lidocaine, 4350 and 4691; GX, 784 and 862; MEGX, 714 and 710. Blood pressure was significantly increased at 30 minutes after high dose infusion. Conclusion and clinical relevance Lidocaine infusions reduced the MAC of I in a dose-dependent manner and did not induce clinically significant changes on heart rate or blood pressure.

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T19:04:20Z No. of bitstreams: 1 S1413-95962004000500001.pdf: 182356 bytes, checksum: 72a7d3e5237a36bd811152f920cb4327 (MD5) Made available in DSpace on 2013-08-22T19:04:20Z (GMT). No. of bitstreams: 1 S1413-95962004000500001.pdf: 182356 bytes, checksum: 72a7d3e5237a36bd811152f920cb4327 (MD5) Previous issue date: 2004-10-01 Made available in DSpace on 2013-09-30T20:07:09Z (GMT). No. of bitstreams: 2 S1413-95962004000500001.pdf: 182356 bytes, checksum: 72a7d3e5237a36bd811152f920cb4327 (MD5) S1413-95962004000500001.pdf.txt: 23099 bytes, checksum: b0ed86f840475b26be2dd667664d2677 (MD5) Previous issue date: 2004-10-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:17:10Z No. of bitstreams: 2 S1413-95962004000500001.pdf: 182356 bytes, checksum: 72a7d3e5237a36bd811152f920cb4327 (MD5) S1413-95962004000500001.pdf.txt: 23099 bytes, checksum: b0ed86f840475b26be2dd667664d2677 (MD5) Made available in DSpace on 2014-05-20T15:17:10Z (GMT). No. of bitstreams: 2 S1413-95962004000500001.pdf: 182356 bytes, checksum: 72a7d3e5237a36bd811152f920cb4327 (MD5) S1413-95962004000500001.pdf.txt: 23099 bytes, checksum: b0ed86f840475b26be2dd667664d2677 (MD5) Previous issue date: 2004-10-01 O presente trabalho teve por objetivo analisar a influência do desenvolvimento etário e da suplementação com acetato de DL-alfa-tocoferol sobre o metabolismo oxidativo de neutrófilos, em bovinos da raça holandesa, no período do nascimento até os 150 dias de idade. Foram utilizados 20 bezerros divididos em dois grupos de dez animais. Os animais do grupo Tratamento receberam 2000UI de acetato de DL-alfa-tocoferol, por via intramuscular, ao nascimento, aos 15, 30, 60, 90 e 120 dias de idade, sendo o outro o grupo Controle, que não recebeu qualquer suplementação. em ambos os grupos, o metabolismo oxidativo dos neutrófilos demonstrou pouca atividade durante os primeiros 60 dias de vida, sendo indicativo da ineficiência deste importante mecanismo bactericida. Não foi observado efeito significativo da administração do acetato de DL-alfa-tocoferol sobre o metabolismo oxidativo de neutrófilos. This study was carried out to evaluate the influence of age and supplementation with acetate of DL-alpha-tocopherol on the neutrophils oxidative metabolism of Holstein calves from birth to 150 days of age. Twenty calves were divided in two groups of ten animals. The animals of group Treatment received 2000UI of DL-alpha-tocopherol acetate, IM, at birth, and at 15, 30, 60, 90 and 120 days of age. The other group (Control), did not receive any supplementation. In both experimental groups the neutrophils oxidative metabolism showed little activity during the first 60 days of life, wich could indicate inefficiency of this important bactericidal mechanism. Neutrophils oxidative metabolism was not significantly affected by treatment with acetate of DL-alpha-tocopherol. Universidade Federal da Bahia (UFBA) Escola de Medicina Veterinária Universidade Federal da Bahia (UFBA) Instituto de Ciências de Saúde Universidade Estadual Paulista Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista Faculdade de Medicina Universidade Estadual Paulista Instituto de Biociências Universidade Estadual Paulista Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista Faculdade de Medicina Universidade Estadual Paulista Instituto de Biociências