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Introduction: The AmpFire HPV genotyping Assay (Atila Biosystems, Mountain View, CA, USA) is a new test for which there are few data regarding its analytic performance and reliability. Using anal and penile swab specimens from a cohort study of men who have sex with men (MSM) in Rwanda, we compared high-risk HPV (hrHPV) detection by AmpFire done at two laboratories, one at University of California San Francisco (UCSF) and the other Rwanda Military Hospital, and well-validated MY09/11-based assay done at UCSF. Methods: Anal and penile specimens collected from 338 MSM from March 2016 to September 2016 were tested for high-risk HPV genotypes (hrHPV) by MY09/11, AmpFire UCSF and AmpFire RMH. Cohen's kappa coefficient was used to test for reproducibility. Results: The hrHPV positivity by MY09/11 and AmpFire UCSF was 13% and 20.7% (k = 0.73) for anal specimens and was 26.3% and 32.6% (k = 0.67) for penile specimens. Specifically, good reproducibility was for types 16 and 18 (k = 0.69 and k = 0.71) for anal specimens and (k = 0.50 and k = 0.72) for penile specimens. The hrHPV positivity by AmpFire at UCSF and RMH was 20.7% for both laboratories (k = 0.87) for anal specimens and was 34.9% and 31.9% (k = 0.89) for penile specimens. Specifically, excellent reproducibility was for types 16 and 18 for anal specimens (k = 0.80 and k = 1.00) and penile specimens (k = 0.85 and k = 0.91). Conclusion: Results show that MY09/11 and AmpFire assays have good reproducibility while the AmpFire UCSF and RMH assays have excellent reproducibility. These results show that AmpFire is a promising HPV genotyping test.

The aims of this study were to: 1) determine prevalence of anogenital and oral HPV, 2) determine concordance between HPV at anal, perianal, scrotal/penile, and oral sites; and 3) describe factors associated with anogenital HPV types targeted by the 9-valent vaccine. Data were collected from 2012 to 2015 among men who have sex with men 18–26 years of age enrolled in a vaccine trial (N = 145). Penile/scrotal, perianal, anal, and oral samples were tested for 61 HPV types. Logistic regression was used to identify factors associated with types in the 9-valent vaccine. Participants’ mean age was 23.0 years, 55.2% were African-American, and 26.2% were Hispanic; 93% had anal, 40% penile, and 6% oral HPV. Among those with anogenital infection, 18% had HPV16. Concordance was low between anogenital and oral sites. Factors independently associated with a 9-valent vaccine-type HPV were: race (African-American vs. White, OR=2.67, 95% CI=1.11–6.42), current smoking (yes vs. no, OR=2.37, 95% CI=1.03–5.48), and number of recent receptive anal sex partners (2+ vs. 0, OR=3.47, 95% CI=1.16–10.4). Most MSM were not infected with HPV16 or HPV18, suggesting that they may still benefit from HPV vaccination, but anogenital HPV was very common, highlighting the importance of vaccinating men before sexual initiation. Clinical trial number: NCT01209325 Keywords: Adolescents, Clinical trials, Men who have sex with men, Vaccine, Human papillomavirus, HIV

Abstract Humans exhibit consistent color preferences that are often described as a curvilinear pattern across hues. The recent literature posits that color preference is linked to the preference for objects or other entities associated with those colors. However, many studies examine this preference using isoluminant colors, which don’t reflect the natural viewing experience typically influenced by different light intensities. The inclusion of random luminance levels (luminance noise) in chromatic stimuli may provide an initial step towards assessing color preference as it is presented in the real world. Employing mosaic stimuli, this study aimed to evaluate the influence of luminance noise on human color preference. Thirty normal trichromats engaged in a two-alternative forced-choice paradigm, indicating their color preferences between presented pairs. The chromatic stimuli included saturated versions of 8 standard hues, presented in mosaics with varying diameters under different luminance noise conditions. Results indicated that the inclusion of luminance noise increased color preference across all hues, specifically under the high luminance noise range, while the curvilinear pattern remained unchanged. Finally, women exhibit a greater sensitivity to the presence of luminance noise than men, potentially due to differences between men and women in aesthetic evaluation strategies.

O objetivo deste trabalho foi avaliar o banco de sementes do solo em áreas em processo de restauração há 20 anos. Foram estudadas 3 áreas, localizadas à jusante da barragem da Usina Hidrelétrica de Camargos, Itutinga, MG, sendo um fragmento de Floresta Estacional Semidecidual (RE) como referência, uma área classificada como degradada (DE) e outra área como perturbada (PE). As avaliações foram realizadas em 3 épocas do ano. Em cada área e a cada avaliação foram coletadas 25 amostras, retirando-se, primeiramente, a serapilheira e, posteriormente, o solo com o auxílio de um gabarito (25 x 25 x 5 cm). O material foi disposto em condições controladas para germinação das sementes. Houve diferença no valor de diversidade de Jaccard entre as três épocas de avaliação. As áreas DE e PE, apesar de possuírem maior diversidade de espécies arbóreas, apresentaram menor número de indivíduos. As espécies pioneiras foram maioria no número de espécies e de indivíduos nas três áreas, como ocorre naturalmente em bancos de sementes, representando mais que 90% dos indivíduos nas três avaliações da área RE. A síndrome de dispersão autocórica predominou nas três áreas e nas três avaliações.

It is common to observe conventional methods for estimating soybean crop yields, making the process slow and susceptible to human error. Therefore, the objective was to develop a model based on deep learning to estimate soybean yield using digital images obtained through a smartphone. To do this, the ability of the proposed model to correctly classify pods that have different numbers of grains, count the number of pods and grains, and then estimate the soybean crop yield was analyzed. As part of the study, two types of image acquisition were performed for the same plant. Image acquisition 1 (IA1) included capturing the images of the entire plant, pods, leaves, and branches. Image acquisition 2 (IA2) included capturing the images of the pods removed from the plant and deposited in a white container. In both acquisition methods, two soybean cultivars, TMG 7063 Ipro and TMG 7363 RR, were used. In total, combining samples from both cultivars, 495 images were captured, with each image corresponding to a sample (plant) obtained through methods AI1 and AI2. With these images, the total number of pods in the entire dataset was 46,385 pods. For the training and validation of the model, the data was divided into subsets of training, validation, and testing, representing, respectively, 80, 10, and 10% of the total dataset. In general, when using the data from IA2, the model presented errors of 7.50 and 5.32% for pods and grains, respectively. These values are considerably lower than when the model used the IA1 data, where it presented errors of 34.69 and 35.25% for pod and grain counts, respectively. Therefore, the data used from IA2 provide better results to the model.

Esse artigo resulta de uma reflexão sobre a construção poética do espaço geográfico a partir da análise da representaçãofílmica do percurso do protagonista do filme Viajo Porque Preciso, Volto Porque Te Amo (Marcelo Gomes e Karim Aïnouz,2009) pela região Nordeste do Brasil. Central para a reflexão é a noção de “poética” que, no caso, toma forma e exerceum papel fundamental no contexto da construção imagética e narrativa do espaço geográfico fílmico, construindo culturalmenteo imaginário coletivo relacionado a ambos os espaços, o real e o representacional.

Prion diseases are disorders of protein conformation that produce neurodegeneration in humans and animals. Studies of transgenic (Tg) mice indicate that a factor designated protein X is involved in the conversion of the normal cellular prion protein (PrPC) into the scrapie isoform (PrPSc); protein X appears to interact with PrPCbut not with PrPSc. To search for PrPCbinding proteins, we fused PrP with alkaline phosphatase (AP) to produce a soluble, secreted probe. PrP–AP was used to screen a λgt11 mouse brain cDNA library, and six clones were isolated. Four cDNAs are novel while two clones are fragments of Nrf2 (NF-E2 related factor 2) transcription factor and Aplp1 (amyloid precursor-like protein 1). The observation that PrP binds to a member of the APP (amyloid precursor protein) gene family is intriguing, in light of possible relevance to Alzheimer's disease. Four of the isolated clones are expressed preferentially in the mouse brain and encode a similar motif.

Papillomaviruses have been implicated in a variety of human diseases ranging from common warts to invasive carcinoma of the anogenital mucosa. Existing assays for genotyping human papillomavirus are restricted to a small number of types. Here, we present a comprehensive, accurate microarray strategy for detection and genotyping of 102 human papillomavirus types and validate its use in a panel of 91 anal swabs. This array has equal performance to traditional dot blot analysis with the benefits of added genotype coverage and the ability to calibrate readout over a range of sensitivity or specificity values.

Sexually transmitted infections (STIs) such as herpes simplex virus (HSV)-2 are associated with an increased risk of HIV infection. Human papillomavirus (HPV) is a common STI, but little is know about its role in HIV transmission. The objective of this study was to determine whether cervico-vaginal HPV infection increases the risk of HIV acquisition in women independent of other common STIs.This prospective cohort study followed 2040 HIV-negative Zimbabwean women (average age 27 years, range 18-49 years) for a median of 21 months. Participants were tested quarterly for 29 HPV types (with L1 PCR primers) and HIV (antibody testing on blood samples with DNA or RNA PCR confirmation). HIV incidence was 2.7 per 100 woman-years. Baseline HPV prevalence was 24.5%, and the most prevalent HPV types were 58 (5.0%), 16 (4.7%), 70 (2.4%), and 18 (2.3%). In separate regression models adjusting for baseline variables (including age, high risk partner, positive test for STIs, positive HSV-2 serology and condom use), HIV acquisition was associated with having baseline prevalent infection with HPV 58 (aHR 2.13; 95% CI 1.09-4.15) or HPV 70 (aHR 2.68; 95% CI 1.08-6.66). In separate regression models adjusting for both baseline variables and time-dependent variables (including HSV-2 status, incident STIs, new sexual partner and condom use), HIV acquisition was associated with concurrent infection with any non-oncogenic HPV type (aHR 1.70; 95% CI 1.02-2.85), any oncogenic HPV type (aHR 1.96; 95% CI 1.16-3.30), HPV 31 (aHR 4.25; 95% CI 1.81-9.97) or HPV 70 (aHR 3.30; 95% CI 1.50-7.20). Detection of any oncogenic HPV type within the previous 6 months was an independent predictor of HIV acquisition, regardless of whether HPV status at the HIV acquisition visit was included (aHR 1.95; 95% CI 1.19-3.21) or excluded (aHR 1.96; 95% CI 1.02-2.85) from the analysis.Cervico-vaginal HPV infection was associated with an increased risk of HIV acquisition in women, and specific HPV types were implicated in this association. The observational nature of our study precludes establishment of causation between HPV infection and HIV acquisition. However, given the high prevalence of HPV infection in women, further investigation of the role of HPV in HIV transmission is warranted.