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Citrus leprosis (CL) is one of the most important viral diseases in sweet orange orchards in Latin America. It is caused by members of at least five species of the so-called Brevipalpus-transmitted viruses (BTV), and the prevalent is Citrus leprosis virus C (CiLV-C). This virus has the broadest host range amongst all CL-associated viruses and is transmitted by Brevipalpus yothersi, a polyphagous mite that can colonize a large variety of host plants, including some spontaneous ground cover plants. But if, on one hand, spontaneous plants can host CL virus and vector, on the other hand, they can offer alternative food for predators, equally common in organic citrus orchards. Brevipalpus yothersi and predator mites were surveyed in 33 spontaneous plants of a Westin sweet orange orchard conducted under organic production system in Brazil, from June 2010 to April 2011. Predatory mites were identified as phytoseiids, and Iphiseiodes zuluagai was the prevalent species, representing 58% of all predators. Other phytoseiids were considered accidental species in the area. Ageratum conyzoides and Alternanthera tenella were the most represented plant host species to predators, comprising 28 and 10% of the total surveyed plants, respectively. Brevipalpus yothersi specimens were detected on various spontaneous species: A. conyzoides, A. tenella, Amaranthus deflexus, Bidens pilosa, Ipomoea quamoclit, I. cairica, Merremia cissoides, Solanum americanum, Panicum maximum, and, predominantly, Commelina benghalensis. The latter has been previously reported as host of CiLV-C as well and, therefore, it is recommended to eliminate this species from citrus orchards.

Citrus leprosis (CL) is one of the most devastating viral diseases of orchards, and industries correspondingly invest highly in the management and control of the virus vector. In Brazil, the disease is caused most predominantly by the citrus leprosis virus C (CiLV-C, Kitaviridae: Cilevirus), and also by citrus leprosis virus N (CiLV-N, Rhabdoviridae: Dichorhavirus). Both viruses are transmitted by false spider mites and at least three different species, Brevipalpus yothersi Baker, B. papayensis Baker, and B. phoenicis (Geijskes) sensu stricto, have been reported in citrus orchards. The main goal of this study was to evaluate the capacity of three Brevipalpus species to transmit citrus leprosis virus (cytoplasmic and nuclear types). The capacity of false spider mites to acquire the virus was accomplished using RT–PCR and the ability to inoculation the virus to host plants (common bean and sweet orange) was assessed via viral transmission assays. Common beans infested with B. yothersi and B. papayensis showed symptoms of CiLV-C in 87.5 and 17% of the plants assessed, respectively. In sweet orange, B. yothersi was exclusively able to inoculate CiLV-C, and around 83% of samples were symptomatic. Host plants infected with CiLV-N showed symptoms only when infested with B. phoenicis sensu stricto (s.s.). All the Brevipalpus species (Acari: Tenuipalpidae) were able to acquire both viruses (CiLV-C and CiLV-N), but not infect plants. These results suggest the existence of virus-vector specificity in the leprosis pathosystem, and this information will be critical for enhancing our further understanding of epidemiological features and disease management.

Reported in Brazil since the 1930’s, citrus leprosis, caused mainly by citrus leprosis virus C, has been a major concern for the national sweet orange production. In recent years, the disease has spread to several other countries and it is now considered a worldwide threat. The occurrence of the disease has been studied almost exclusively in sweet oranges because other citrus genotypes are of secondary relevance in Brazil and in some other American countries where it occurs. Here we report 12 resistant citrus genotypes among 160 accessions evaluated. After 90 days of the infestation with viruliferous mites, asymptomatic genotypes were observed in sour orange, lemon, grapefruit, mandarins, tangelo, and tangor groups. The results revealed promising genotypes resistant to the disease, which can be incorporated in citrus breeding programs aiming to obtain varietal resistance, and confirmed the susceptibility of many citrus genotypes to CiLV-C. This assay ratify the already reported uneven level of susceptibility within the citrus group.

Brevipalpus-transmitted viruses (BTV) are a taxonomically diverse group of plant viruses which severely affect a number of major crops. Members of the group can be sub-classified into cytoplasmic (BTV-C) or nuclear type (BTV-N) according to the accumulation sites of virions in the infected plant cells. Both types of BTV produce only local infections near the point of inoculation by viruliferous mites. Features of BTV-plant interactions such as the failure of systemic spread in their natural hosts are poorly understood. In this study we evaluated Arabidopsis thaliana, a model plant commonly used for the study of plant-virus interactions, as an alternative host for BTV. Infection of Arabidopsis with the BTV-N Coffee ringspot virus and Clerodendrum chlorotic spot virus, and the BTV-C Solanum violaefolium ringspot virus, were mediated by viruliferous Brevipalpus mites collected in the wild. Upon infestation, local lesions appeared in 7 to 10 days on leaves of, at least, 80 % of the assayed plants. Presence of viral particles and characteristic cytopathic effects were detected by transmission electron microscopy (TEM) and the viral identities confirmed by specific reverse-transcriptase polymerase chain reaction (RT-PCR) and further amplicon sequencing. The high infection rate and reproducibility of symptoms of the three different viruses assayed validate A. thaliana as a feasible alternative experimental host for BTV.

Made available in DSpace on 2013-08-12T18:45:47Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-07-01 Made available in DSpace on 2013-09-30T17:53:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-07-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:14:50Z No. of bitstreams: 0 Made available in DSpace on 2014-05-20T13:14:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-07-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) USDA-ARS Fundecitrus The equivalent of US$75 million is spent each year in Brazil to control Brevipalpus phoenicis, a mite vector of Citrus leprosis virus C (CiLV-C). In this study, we investigated the possibility that hedgerows and windbreaks normally found in citrus orchards could host CiLV-C. Mites confined by an adhesive barrier were reared on sweet orange fruit with leprosis symptoms then were transferred to leaves of Hibiscus rosa-sinensis, Malvaviscus arboreus, Grevilea robusta, Bixa orellana, and Citrus sinensis. Ninety days post infestation, the descendant mites were transferred to Pera sweet orange plants to verify the transmissibility of the virus back to citrus. Nonviruliferous mites which had no feeding access to diseased tissue were used as controls. Local chlorotic or necrotic spots and ringspots, symptoms of leprosis disease, appeared in most plants tested. Results generated by reverse-transcription polymerase chain reaction with primers specific for CiLV-C and by electron microscope analyses confirmed the susceptibility of these plants to CiLV-C. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Cassava & Fruits Ctr APTA Citros Sylvio M, BR-13490970 Cordeiropolis, SP, Brazil FCAV UNESP, Depto Fitossanidade, BR-14884900 Jaboticabal, SP, Brazil ESALQ, Depto Fitopatol & Nematol, BR-13418900 Piracicaba, SP, Brazil ARS, USDA, USHRL, Ft Pierce, FL USA FCAV UNESP, Depto Fitossanidade, BR-14884900 Jaboticabal, SP, Brazil

Brevipalpus phoenicis s.l. is an economically important vector of the Citrus leprosis virus-C (CiLV-C), one of the most severe diseases attacking citrus orchards worldwide. Effective control strategies for this mite should be designed based on basic information including its population structure, and particularly the factors that influence its dynamics. We sampled sweet orange orchards extensively in eight locations in Brazil and 12 in Mexico. Population genetic structure and genetic variation between both countries, among locations and among sampling sites within locations were evaluated by analysing nucleotide sequence data from fragments of the mitochondrial cytochrome oxidase subunit I (COI). In both countries, B. yothersi was the most common species and was found in almost all locations. Individuals from B. papayensis were found in two locations in Brazil. Brevipalpus yothersi populations collected in Brazil were more genetically diverse (14 haplotypes) than Mexican populations (four haplotypes). Although geographical origin had a low but significant effect (ca. 25%) on the population structure, the greatest effect was from the within location comparison (37.02 %). Potential factors driving our results were discussed.

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:42:51Z No. of bitstreams: 1 S1676-06032009000400026.pdf: 112580 bytes, checksum: cdd707af8f1ebf0249ebb668eec6d35b (MD5) Made available in DSpace on 2013-08-22T18:42:51Z (GMT). No. of bitstreams: 1 S1676-06032009000400026.pdf: 112580 bytes, checksum: cdd707af8f1ebf0249ebb668eec6d35b (MD5) Previous issue date: 2009-12-01 Made available in DSpace on 2013-09-30T19:30:31Z (GMT). No. of bitstreams: 2 S1676-06032009000400026.pdf: 112580 bytes, checksum: cdd707af8f1ebf0249ebb668eec6d35b (MD5) S1676-06032009000400026.pdf.txt: 15854 bytes, checksum: c4686de5c9ff5ff870495b12e9c5e953 (MD5) Previous issue date: 2009-12-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:08:22Z No. of bitstreams: 2 S1676-06032009000400026.pdf: 112580 bytes, checksum: cdd707af8f1ebf0249ebb668eec6d35b (MD5) S1676-06032009000400026.pdf.txt: 15854 bytes, checksum: c4686de5c9ff5ff870495b12e9c5e953 (MD5) Made available in DSpace on 2014-05-20T15:08:22Z (GMT). No. of bitstreams: 2 S1676-06032009000400026.pdf: 112580 bytes, checksum: cdd707af8f1ebf0249ebb668eec6d35b (MD5) S1676-06032009000400026.pdf.txt: 15854 bytes, checksum: c4686de5c9ff5ff870495b12e9c5e953 (MD5) Previous issue date: 2009-12-01 Embora seja geralmente assumido que a agricultura influencia negativamente populações de anfíbios, existem poucos estudos sobre os efeitos dos cultivos agrícolas em anuros neotropicais. Visando contribuir para diminuir essa lacuna de conhecimento, no presente estudo buscamos verificar quais espécies de anuros estão presentes nos agrossistemas. Para isso, usamos dados de anuros capturados em armadilhas de queda, inicialmente proposto para o levantamento da fauna de opiliões em três agrossistemas (milho, soja e seringal). Nós registramos quatro espécies de anuros nas armadilhas de queda: Leptodactulus fuscus, L. mystacinus (Leptodactylidae), Eupemphix nattereri e Physalaemus cuvieri (Leiuperidae). Na plantação de milho foram registradas quatro espécies e 30 indivíduos, no seringal quatro espécies e 11 indivíduos e na plantação de soja três espécies e oito indivíduos. Nossos resultados mostram que os anuros estão presentes nos agrossistemas, principalmente espécies de anuros generalistas. Although it is generally assumed that agriculture negatively influences amphibian populations, few studies on the effects of agricultural cultivations on neotropical anuran have been conducted. As a contribution to the knowledge about anuran in agriculture, the present study sought to identify the anuran species present in three different agrossystems. We used data from anurans captured in pitfall traps initially proposed for a survey of harvestmen fauna in three agrossystems (corn, soybean, and rubber tree). Four anuran species found in the pitfall traps belong to two Families: Leptodactylidae: Leptodactulus fuscus and L. mystacinus; and Leiuperidae: Eupemphix nattereri and Physalaemus cuvieri. In corn plantation, four species and 30 individuals were captured; in rubber trees, four species and 11 individuals; and in soybeans plantation, tree species and eight individuals. Our results show that anurans are present in agrossystems, mainly the generalist anuran species. Universidade Estadual Paulista Programa de Pós Graduação em Biologia Animal Faculdades Integradas - FACVEST Universidade Estadual Paulista Universidade Estadual Paulista Departamento de Zoologia e Botânica Universidade Estadual Paulista Programa de Pós Graduação em Biologia Animal Universidade Estadual Paulista Universidade Estadual Paulista Departamento de Zoologia e Botânica

There are two bacilliform, rhabdo-like viruses that cause citrus leprosis: Citrus leprosis virus C (CiLV-C), which accumulates in the cytoplasm of infected cells, and Citrus leprosis virus nuclear type (CiLV-N), which accumulates in their nucleus. The first one, the prototype of the new genus Cilevirus, is prevalent and occurs in several countries of the American continent, from Argentina to Mexico (1). The second type, still a tentative member of the Rhabdoviridae family, is of rare occurrence, with a few reports in Brazil and one in Panama (1). Leprosis is particularly important to the Brazilian citrus industry because of the 60 to 80 million dollars spent yearly for the control of Brevipalpus phoenicis (Geijskes, 1939) (Acari: Tenuipalpidae), the vector of the virus (1). For decades, the disease was considered unique to citrus plants; however, greenhouse experiments conducted in the 1990s demonstrated the mechanical transmission of CiLV-C to noncitrus plants (1). Years later, researchers were able to transmit the virus to nonrutaceous hosts using viruliferous mites (1,4). Recently, León et al. (2) reported the occurrence of the first noncitrus plant naturally infected by CiLV-C, the rutaceous Swinglea glutinosa Blanco (Merr.). Tropical spiderworts (Commelina benghalensis L.; Commelinaceae) are monocot weeds commonly found in citrus orchards in Brazil. In a survey conducted in orchards with high incidences of leprosis in the municipalities of Borborema and São José do Rio Preto, State of Sao Paulo, Brazil, tropical spiderworts were found exhibiting necrotic spots with a yellow halo in green leaves and green spots with necrotic center in senescent leaves. Since these symptoms are similar to those caused by CiLV-C in citrus, symptomatic plants were collected and subjected to transmission electron microscopy analyses and reverse transcription-PCR using primers that specifically amplify a region within the putative movement protein gene of the virus (3). Bacilliform virus particles and typical inclusions were seen in the lesions. Bands of the expected 344 bp size were seen in agarose gels of symptomatic samples only. The analysis of the consensus sequence showed 100% identity with CiLV-C sequence available in the GenBank (Accession No. YP_654542.1). Experimental transmission of CiLV-C by B. phoenicis reproduced the lesions in inoculated tropical spiderwort. Also, the virus could be easily transmitted back from C. benghalensis to sweet orange plants. Our data show that this widespread weed is the first monocot as a natural host for CiLV-C. Since tropical spiderwort is a host for B. phoenicis and symptomatic plants were found in two municipalities 130 km apart from each other, it is possible that this weed may serve as reservoir for the virus and play a relevant role in the spread of the disease in the field, but this issue still needs to be addressed in further experiments. References: (1) M. A. Bastianel et al. Plant Dis. 94:284, 2010. (2) M. G. A. Leon et al. Plant Dis. 90:682, 2008. (3) E. C. Locali-Fabris et al. Plant Dis. 87:1317. (4) M. A. Nunes et al. Plant Dis. Online publication. doi:10.1094/PDIS-06-11-0538, 2011.

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:52:42Z No. of bitstreams: 1 S0006-87052009000400020.pdf: 791441 bytes, checksum: 910f48d54de3f7bcc60e3da33b23d512 (MD5) Made available in DSpace on 2013-08-22T18:52:42Z (GMT). No. of bitstreams: 1 S0006-87052009000400020.pdf: 791441 bytes, checksum: 910f48d54de3f7bcc60e3da33b23d512 (MD5) Previous issue date: 2009-01-01 Made available in DSpace on 2013-09-30T17:51:42Z (GMT). No. of bitstreams: 2 S0006-87052009000400020.pdf: 791441 bytes, checksum: 910f48d54de3f7bcc60e3da33b23d512 (MD5) S0006-87052009000400020.pdf.txt: 43651 bytes, checksum: 078df2ec22f9b8da9a8a2f9fd86f2edd (MD5) Previous issue date: 2009-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:13:52Z No. of bitstreams: 2 S0006-87052009000400020.pdf: 791441 bytes, checksum: 910f48d54de3f7bcc60e3da33b23d512 (MD5) S0006-87052009000400020.pdf.txt: 43651 bytes, checksum: 078df2ec22f9b8da9a8a2f9fd86f2edd (MD5) Made available in DSpace on 2014-05-20T13:13:52Z (GMT). No. of bitstreams: 2 S0006-87052009000400020.pdf: 791441 bytes, checksum: 910f48d54de3f7bcc60e3da33b23d512 (MD5) S0006-87052009000400020.pdf.txt: 43651 bytes, checksum: 078df2ec22f9b8da9a8a2f9fd86f2edd (MD5) Previous issue date: 2009-01-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) O conhecimento da diversidade e distribuição de artrópodes associados ao solo contribui para o desenvolvimento de sistemas agrícolas sustentáveis. O presente estudo foi realizado em Jaboticabal (SP), durante o período de fevereiro a abril de 2004. O objetivo foi analisar a comunidade de Carabidae, Staphylinidae, Formicidae e Araneae através de índices faunísticos e determinar a distribuição espacial e a interação interespecífica de espécies predominantes em soja (Glycine max (L.) Merr.), milho (Zea mays L.) e seringueira (Hevea brasiliensis Muell. Arg.). Os artrópodes foram amostrados com armadilhas de solo distribuídas a cada 10 m em dois transectos de 210 m de comprimento, que atravessaram o seringal e avançaram 60 m no interior das culturas. A fauna foi caracterizada pelos índices de diversidade de Shannon-Wiener, de equitabilidade e de similaridade de Morisita. As diferenças entre a ocorrência das espécies predominantes nos hábitats foram determinadas por análise de variância e a interação interespecífica por correlação de Pearson. A soja e o milho cultivados em sistema de plantio direto propiciaram comunidades de carabídeos, formigas e aranhas mais bem estruturadas que o seringal. Entre as 88 espécies capturadas, 20 espécies foram predominantes cuja distribuição espacial mostrou que Odontocheila nodicornis (Dejean), Glenus chrysis Gravenhorst, Castianeira sp. e oito espécies de formigas foram mais abundantes no seringal em comparação às culturas de soja e do milho. A abundância dos carabídeos Calosoma granulatum Perty e O. nodicornis diminuiu conforme aumentou a densidade dos formicídeos Pheidole sp.1 e Odontomachus chelifer Latreille respectivamente. The knowledge of the diversity and distribution of ground arthropods contributes for the development of sustainable agricultural systems. This work was carried out at the Paulista State University, Jaboticabal campus, State of São Paulo, Brazil, during the period from February to April 2004. The objective was to analyse the community of Carabidae, Staphylinidae, Formicidae e Araneae through faunistic indexes, to determine the spatial distribution and interespecific interactions of predominant species in soybean (Glycine max (L.) Merr.), corn (Zea mays L.) and rubber tree (Hevea brasiliensis Muell. Arg.). The arthropods were sampled by pitfall traps distributed each 10 m in two transects of 210 m which crossed the rubber trees and advancing 60 m in the crop lines. The fauna was characterized by Shannon-Wiener diversity index, evenness and Morisita similarity index. The differences among the occurrence of predominant species in the habitats were determined by variance analysis, and the interespecific interaction by Pearson correlation. The soybean and corn under no-tillage system provided better structured carabids, ants and spiders communities than the rubber trees. Among 88 captured species, 20 species were predominant and the spatial distribution of them showed that Odontocheila nodicornis (Dejean), Glenus chrysis Gravenhorst, Castianeira sp. and eight ant species were more abundant in the rubber tree compared to soybean and corn. The abundance of the carabids Calosoma granulatum Perty and O. nodicornis decreased with increased density of the formicids Pheidole sp.1 and Odontomachus chelifer Latreille, respectively. UNESP Faculdade de Ciências Agrárias e Veterinárias Departamento de Fitossanidade UNESP Faculdade de Ciências Agrárias e Veterinárias Departamento de Ciências Exatas CNPq Produtividade em Pesquisa UNESP Faculdade de Ciências Agrárias e Veterinárias Departamento de Fitossanidade UNESP Faculdade de Ciências Agrárias e Veterinárias Departamento de Ciências Exatas

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:47:49Z No. of bitstreams: 1 S0100-29452007000200037.pdf: 641974 bytes, checksum: 2773155454178493f83138e5fb19e89b (MD5) Made available in DSpace on 2013-08-22T18:47:49Z (GMT). No. of bitstreams: 1 S0100-29452007000200037.pdf: 641974 bytes, checksum: 2773155454178493f83138e5fb19e89b (MD5) Previous issue date: 2007-08-01 Made available in DSpace on 2013-09-30T19:37:32Z (GMT). No. of bitstreams: 2 S0100-29452007000200037.pdf: 641974 bytes, checksum: 2773155454178493f83138e5fb19e89b (MD5) S0100-29452007000200037.pdf.txt: 16770 bytes, checksum: df00dc7fae86cad637df8b2b2245b2b3 (MD5) Previous issue date: 2007-08-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:16:24Z No. of bitstreams: 2 S0100-29452007000200037.pdf: 641974 bytes, checksum: 2773155454178493f83138e5fb19e89b (MD5) S0100-29452007000200037.pdf.txt: 16770 bytes, checksum: df00dc7fae86cad637df8b2b2245b2b3 (MD5) Made available in DSpace on 2014-05-20T13:16:24Z (GMT). No. of bitstreams: 2 S0100-29452007000200037.pdf: 641974 bytes, checksum: 2773155454178493f83138e5fb19e89b (MD5) S0100-29452007000200037.pdf.txt: 16770 bytes, checksum: df00dc7fae86cad637df8b2b2245b2b3 (MD5) Previous issue date: 2007-08-01 O objetivo deste trabalho foi avaliar a forma de ação de duas preparações de extrato pirolenhoso aplicadas diretamente sobre Brevipalpus phoenicis, que é o ácaro vetor da leprose dos citros, um dos principais problemas da citricultura Paulista. Para o experimento, foram utilizados ácaros adultos mantidos numa criação-estoque no laboratório de Acarologia da UNESP - Universidade Estadual Paulista, em Jaboticabal-SP. Os tratamentos foram constituídos por duas diferentes preparações (destilado e decantado) de extrato pirolenhoso de eucalipto nas proporções EP:água de 1:600; 1:300 (normalmente recomendadas); 1:150; 1:75; 1:38; 1:19 e de água (testemunha), com 7 repetições. Cada parcela foi constituída de 10 ácaros mantidos sobre um fruto de laranja, em arena de 2,5 cm de diâmetro, delimitada com cola adesiva tipo Tanglefoot®. As aplicações foram efetuadas em Torre de Potter, pulverizando-se 2 mL por fruto das soluções correspondentes aos diferentes tratamentos. Os frutos foram mantidos em sala climatizada a 27±1ºC, e as avaliações foram realizadas 24 e 48 horas após a aplicação dos tratamentos, determinando-se o número de ácaros mortos (mortalidade) e retidos na barreira adesiva (repelência). Os dois tipos de extrato pirolenhoso testados não apresentaram repelência significativa sobre Brevipalpus phoenicis; ambos induziram mortalidade significativa somente para concentrações acima de 1:150, com efeito mais pronunciado para o destilado; há um aumento na mortalidade de 24 para 48 horas após a aplicação; a ação protetora preconizada pela aplicação de baixas doses (1:300 a 1:600) nas plantas não é devida à mortalidade e repelência pelo contato direto do extrato pirolenhoso sobre os ácaros. The aim of this work was to evaluate the acaricide and repellent effects of two different pyroligneous extract preparations (PE) applied directly on Brevipalpus phoenicis. This mite is the vector or citrus leprosies, which is one of the main problems of São Paulo State citrus culture. This experiment was carried out with mites derived of the colony-supply maintained in the laboratory of Acarology of the Unesp - São Paulo State University, at Jaboticabal. The experiment consisted of application of water (control) and two distinct way of preparations (distilled and decanted) of pyroligneous extract (PE) of eucalyptus in 6 volumetric ratios PE: water of 1:600, 1:300, 1:150, 1:75, 1:38 and 1:19, with 7 replications. Each plot with 10 mites maintained on an orange fruit, in enclosure of 2.5 cm diameter isolated by Tanglefoot® (The Tanglefoot Company: USA) adhesive barrier. After confection of the enclosures and mites transference, 2 mL of the solutions corresponding to the different treatments were applied per fruit, by using a Potter´s Tower. Then, the fruits were maintained in a room at 27±1ºC, and evaluations of the number of dead mites (mortality) and restrained in the adhesive barrier (repellence) were carried out 24 and 48 hours after treatment application. The distilled PE presented more mortality in comparison to decanted PE for concentrations higher than 1:150. None of two types of extract showed significant repellence effect on B. phoenicis. The protective action recognized for low doses (1:300 to 1:600, normally recommended), is not due to mortality or repellence by direct contact of these products with mites. Universidade Estadual Paulista Universidade Estadual Paulista Departamento de Tecnologia Universidade Estadual Paulista Departamento de Fitossanidade Universidade Estadual Paulista Universidade Estadual Paulista Departamento de Tecnologia Universidade Estadual Paulista Departamento de Fitossanidade

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:54:39Z No. of bitstreams: 1 S0101-81752001000400020.pdf: 3520787 bytes, checksum: 6940d2b2aab89b9e6588f0bc7cbb0f97 (MD5) Made available in DSpace on 2013-08-22T18:54:39Z (GMT). No. of bitstreams: 1 S0101-81752001000400020.pdf: 3520787 bytes, checksum: 6940d2b2aab89b9e6588f0bc7cbb0f97 (MD5) Previous issue date: 2001-12-01 Made available in DSpace on 2013-09-30T19:46:36Z (GMT). No. of bitstreams: 2 S0101-81752001000400020.pdf: 3520787 bytes, checksum: 6940d2b2aab89b9e6588f0bc7cbb0f97 (MD5) S0101-81752001000400020.pdf.txt: 35286 bytes, checksum: 696aa0ceae2295ed3cff08292f6bf223 (MD5) Previous issue date: 2001-12-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T14:03:34Z No. of bitstreams: 2 S0101-81752001000400020.pdf: 3520787 bytes, checksum: 6940d2b2aab89b9e6588f0bc7cbb0f97 (MD5) S0101-81752001000400020.pdf.txt: 35286 bytes, checksum: 696aa0ceae2295ed3cff08292f6bf223 (MD5) Made available in DSpace on 2014-05-20T14:03:34Z (GMT). No. of bitstreams: 2 S0101-81752001000400020.pdf: 3520787 bytes, checksum: 6940d2b2aab89b9e6588f0bc7cbb0f97 (MD5) S0101-81752001000400020.pdf.txt: 35286 bytes, checksum: 696aa0ceae2295ed3cff08292f6bf223 (MD5) Previous issue date: 2001-12-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) This paper reports twenty mite species belonging to eighteen genera in nine families, associated with three species of euphorbiaceous weed species: Chamaesyce hirta (Linnaeus) Millsp., Euphorbia heterophylla L. and Phyllanthus tenellus (Muell. Arg.) Roxb., in three different monoculture areas of Hevea brasiliensis. The largest richness of mite species was observed on C. hirta, with nine species. The most abundant species were Pronematus sp. and Polyphagotarsonemus latus (Banks, 1904), collected on C. hirta and E. heterophylla. Universidade Estadual Paulista Departamento de Zoologia e Botânica Universidade Estadual Paulista Departamento de Zoologia e Botânica