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Dairy calves are commonly reared without contact with their dam, which facilitates a human-animal relationship based on close human contact and feeding. Dam-contact may negatively affect calves’ relationship with humans. The current study investigates the effect of dam-contact and weaning method on calves’ response to humans. A total of 69 dairy calves were allocated to one of three dam-contact treatments [Control (separated from dam after 24 h), Whole-day (housed with dam for 23 h/d), and Half-day (housed with dam for 10 h/d)]. Within each treatment, calves were allocated to one of two weaning treatments [Stepwise (weaning off milk at eight weeks, dam-separation/pen change at nine weeks) or Simultaneous (weaning off milk and dam-separation/pen change simultaneously at nine weeks), i.e Control were weaned in the same manner but only the pen change was possible at the separation step, as calves were already separated from the dam]. All animals received a similar amount of human contact, except control calves who were additionally fed milk by teat bucket twice a day. Calves were tested in a random order within block using a human approach test followed by an animal approach test conducted in a 2.5 m x 10 m arena at 10 weeks of age. Stepwise-Control calves had shorter latencies to first approach the test person than Stepwise-Whole-day (p < 0.05, median survival time of Stepwise-Control: 11 s, Stepwise-Whole-day: 111 s and Stepwise-Half-day: 52 s). Among Simultaneous calves, no dam-contact treatment differences were detected for the latency to first approach. Similarly, Stepwise-Control calves had an odds ratio (95% CI) of 24.2 (1.6–365.9, p < 0.05) for coming within 1 m of the test person vs Stepwise-Whole-day calves and 12.5 (1.1–141.1, p < 0.05) vs Stepwise-Half-day calves. Throughout the test period Simultaneous-Control vocalised less [estimated mean no. of vocalisations (95% CI), 3.6 (2.1–6.4)] than both Simultaneous-Whole-day [18.2 (12.8–25.9), p < 0.01] and Simultaneous-Half-day [15.7 (11.0–22.5), p < 0.01] while there was no difference under Stepwise. As expected, Control approached faster and were more likely to come close to the test person than dam-reared calves, but exclusively after the stepwise weaning and separation. For calves tested one week after simultaneous weaning and separation no effect of the dam-contact treatments was found, except a higher frequency of vocalisations for dam-reared calves. This implies that controlling for the stress level related to weaning and separation from the dam is important when interpreting human-animal relationship tests, as dam-contact treatment effects appeared to be affected by high levels of weaning stress.

Dairy calves are often offered milk allowance at a flat rate during the first 6 wk of life, although an initial high allowance followed by a stepwise reduction (step-down strategy) may correspond better to the calves' nutritional needs. The amount of milk offered per feeding is typically constant. However, during natural suckling, the daily number of milk meals (suckling frequency) declines with age, which may reflect increased consumption of solid feeds. Thus, allowing calves to pattern their meals on a computer-controlled feeder by placing less restriction on meal frequency may stimulate dairy calves' ingestion of solid feeds. This experiment investigated the effects of milk feeding strategy and milk feeding frequency on calves' feeding behavior, intake, and growth. Sixty-four male Holstein Friesian calves, purchased from conventional dairy farms, were housed in groups of 8 and fed milk replacer (MR) via computer-controlled milk feeders. From 14 d old, calves were assigned to either a conventional flat-rate milk allowance (CON: 6.5 L/d of MR from d 14 to 42; 4 L/d from d 43 to 49; 2 L/d from d 50 to 56 of age) or to stepwise reduction in milk allowance (STEP: 8 L/d of MR from d 14 to 28; 5 L/d from d 29 to 42; 4 L/d from d 43 to 49; 2 L/d from d 50 to 56 of age). Within each group of 8, 4 calves were randomly allocated to each of 2 milk feeding frequencies, either restricted portion size (RES: maximum milk portion size of 2.3 L/portion) or unrestricted portion size (UNRES). Concentrates, hay, and water were available ad libitum. Feeding behavior was recorded via video for 24 h on 26 and 40 d of age. On d 26, where STEP calves were offered 1.5 L/d more milk than CON calves, no difference was seen regarding the time spent eating concentrate (16.9 vs. 20.3 min/d). However, STEP calves spent more time eating concentrate on d 40, where these calves had 1.5 L/d less milk than CON (36.1 vs. 27.2 min/d). Thus, a lower daily milk allowance stimulated feeding on concentrate d 40, but not d 26. As predicted, UNRES calves spent more time eating concentrate (27.6 vs. 21.9 min/d) and more time eating hay (38.4 vs. 30.0 min/d) than RES calves. However, higher appetite for solid feeds by UNRES calves may not be explained by milk intake per se. Rather, this is likely due to their opportunity to have larger milk meals, which appeared to increase their appetite for milk (as indicated by longer time spent in the milk feeder; 64.0 vs. 48.8 min/d) as well as for solid feeds. Among CON calves, the UNRES treatment resulted in higher ADG 2 wk postweaning, but not among STEP calves. These findings partially support that relaxing restriction on milk portions helps calves to transition from milk to solid feeds before weaning off milk. However, more research is needed to determine how restriction on milk portions interacts with milk feeding strategy when a higher total milk allowance is offered.

Housing in hospital pens may be beneficial for lame cows due to soft flooring and less competition for resources. We compared recovery from lameness among dairy cows housed in designated hospital pens, with deep-litter straw, with recovery among cows housed together with the rest of the lactating cows in their home pens, with cubicles and slatted or solid concrete floors. Additionally, we compared lying behavior in the 2 groups of cows. A total of 168 lame dairy cows from 5 herds were included in the study. Each herd was visited once weekly, and lame cows (locomotion score 3 or 4 on a 5-point scale) were examined in a hoof-trimming chute, trimmed, and randomly assigned to 1 of 2 treatments: housing in a hospital pen (termed “treatment”; 72 cows) or housing under the herds’ standard conditions together with the rest of the lactating cows in the herd (termed “control”; 96 cows). Cows were locomotion scored weekly until they were no longer lame or until they had been part of the trial for 3 weeks (i.e., 2, 3, or 4 locomotion scorings per cow). We categorized cows to describe the progression of lameness over time: recovery (divided into fast, medium, or slow), improvement without recovery, constant lameness, or worsening of lameness. Lying behavior was recorded in a sample of 60 of the 168 cows for a period of 5 d. Overall, recovery from lameness was significantly different between treatment and control cows. The proportion of cows included in the study with locomotion score 4 and a subsequent improvement was significantly higher among treatment cows than among control cows. Among cows included with locomotion score 4, 40% of treatment cows also had a locomotion score 4 at the fourth locomotion scoring, 46% had improved to a score 3, and 14% were no longer lame. In comparison, 73% of control cows had a locomotion score 4 at the fourth locomotion scoring, 16% had improved to a score 3, and 11% were no longer lame. We found no differences between treatment and control cows for mean daily lying time, number of steps per day, number of daily lying bouts, or mean duration of lying bouts. Housing of lame dairy cows in a hospital pen with a soft surface, easier access to feed and water, a smaller group size, and reduced waiting time for milking may have positive effects on recovery from lameness. Typically, farmers have housed only severely lame cows in hospital pens. However, our results indicate that less severely lame cows may also benefit from a stay in a hospital pen.

The importance of drinking water for production and animal welfare is widely recognized, but surveys and animal welfare assessment schemes suggest that many dairy calves and dairy cows do not have sufficient access. Limit milk-fed calves drink more water than calves fed milk ad libitum, but ad libitum milk-fed calves also require access to drinking water, as milk does not meet the animal's requirement for water. At hot ambient temperatures and when calves are sick, access to water is especially important and should be provided at all times. Many young calves do not have access to water throughout 24 h, and whether healthy young calves require free access to water at all times, or from which age, is not clear and requires further study. Dairy cow free water intake (FWI) is largely determined by milk yield, and high-yielding dairy cows may drink up 100 L of water per day. Dry matter, crude protein, and salt content of feed, as well as ambient temperature, have considerable effects on dairy cow water intake. Deprivation of water affects meal patterning for the cow, as well as increased subsequent rate of drinking and compensatory water intake. Although dairy cow ad libitum water intake may exceed the water provision necessary to maintain production, offering water for ad libitum intake may be necessary to safe guard animal welfare. Cattle are suction drinkers that prefer to drink from large open water surfaces, and Holstein dairy cows can drink at a rate of up to 24 L/min. Research on the effect of design and placement of water troughs for indoor-housed dairy cows on their drinking behavior and water intake is limited. Access to a water source at pasture increases the time cows spend there, and access to shade reduces water requirements during periods of warm weather. In both indoor and pastured cattle, there is a lack of knowledge about the effect of stocking of water troughs on competition, drinking behavior, and intake in dairy cows. Studies on the effect of available water trough length and placement, and of the number of cows being able to drink from the same trough of a given dimension, are needed to evaluate current recommendations.

Abrupt and gradual dry-off strategies by reducing feeding level (normal vs. reduced energy density), reducing milking frequency (twice vs. once daily), and administration of a dopamine agonist after last milking (i.m. saline vs. cabergoline injection) were investigated (2 × 2 × 2 factorial arrangement) for their effects on feed intake, milk yield, energy balance, milk leakage, and clinical udder characteristics in 119 Holstein cows. In the last week before dry-off, cows were assigned to 1 of 4 combinations of feeding level and milking frequency. Within 3 h after last milking, cows were injected with either saline or a dopamine agonist (cabergoline; Velactis, Ceva Santé Animale; labeled for use only with abrupt dry-off, i.e., no preceding reduction in feeding level or milking frequency before last milking). After dry-off, all cows were fed the same diet for dry cows, and data collection continued for a week. Dry matter intake (DMI) was recorded in automated feed bins and milk yield in an automatic milking system where additional concentrate was fed. Clinical udder characteristics and milk leakage were scored 10 times during the week before and the week after dry-off. Before dry-off, total DMI decreased with reduced feeding level compared with normal feeding level, but did not differ between milking frequencies. The combined effect of reduced DMI and diet energy concentration resulted in a 47% lower net energy intake with reduced feeding level compared with normal feeding level during the week before dry-off. Milk yield was approximately 30% lower during the week before dry-off when either feeding level or milking frequency was reduced compared with no change in feeding level or milking frequency, whereas milk yield was 45% lower when both feeding level and milking frequency were reduced. The net energy balance during the week before dry-off was negative with reduced feeding level and more negative when combined with twice-daily milking. After dry-off, udder engorgement was reduced in the 3 gradual dry-off treatments compared with abrupt dry-off. Cabergoline injection after last milking resulted in least udder engorgement and signs of milk leakage for 48 h, but also resulted in abrupt reduction of DMI lasting approximately 24 h irrespective of treatment before dry-off. In conclusion, gradual cessation of lactation by reducing milking frequency to once daily without reducing the feeding level decreased milk yield before dry-off in high-yielding dairy cows and reduced udder engorgement after dry-off without inducing negative energy balance during the period of dry-off. In contrast, reduced feeding level induced negative energy balance, which may compromise welfare due to metabolic stress and hunger. No clear differences in risk of milk leakage after dry-off were observed between abrupt and gradual dry-off management strategies. Use of cabergoline led to fewer signs of milk leakage and reduced udder engorgement during the first days after dry-off, which may positively affect welfare at dry-off. However, the mechanism behind and the welfare consequences of the concomitant abrupt decrease in DMI lasting approximately 24 h needs further investigation to complete our understanding of dopamine agonist use for dry-off. ispartof: JOURNAL OF DAIRY SCIENCE vol:104 issue:10 pages:11108-11125 ispartof: location:United States status: published

Use of individual maternity pens for dairy cows is rec- ommended to ensure undisturbed calving and postpar- tum transfer of colostrum to calves, but management of these pens is a challenge due to difficulty predicting the time of calving. If group-housed cows, however, have the opportunity to calve in a secluded area of a group pen, this may limit disturbance of the dam and her newborn, especially if this area is fitted with a gate preventing other cows from entering. The aims of this study were to assess effects of cows calving inside a secluded area versus cows calving in the group area on maternal behavior and social interaction between dam and herd members during the first postpartum hour, calf’s latency to stand, number of calves suckling an alien cow, and the dam’s acute response to separation from the calf. This was investigated in pens consisting of a central group area with access to 6 secluded areas with or without a mechanical push gate allowing only 1 cow access at a time. At least 2 wk before expected calving, 60 Danish Holstein multiparous cows were as- signed alternately, in groups of 6, to a group pen with 6 gated secluded areas or a group pen with 6 ungated se- cluded areas. The effects of treatment (gated, ungated) and calving place chosen within treatment (secluded area, group area) on maternal behavior, interactions with herd members, maternal response to separation, and calf latency to stand were investigated. Irrespective of whether cows calved in a secluded area or not and whether the secluded area was gated or not, cows spent, on average, 39 (±7.9) min of the first hour after calving licking their calves. When calving in a gated secluded area, the cows interacted less with herd members than when calving in the group area (0.5, 28, 18, and 33 min/h for gated/secluded, gated/group, ungated/secluded, and ungated/group areas, respectively), and no calves suckled an alien cow within the first hour after birth. In addition, the longer the calf stayed with the dam, the longer the dam stood with her head out of the pen after removal of the calf. Calf’s latency to stand after calving was unaffected by treatment and calving place (mean = 39 min; range = 8–243 min). The results show that dam and calf can be protected from distur- bances when cows calve in a secluded maternity area, especially if the secluded maternity area is fitted with a gate preventing other cows form entering.

Dairy calves are often housed in individual pens, which limits play behaviour. This study investigates whether the motivation for social play builds up during periods of deprivation hereof. Sixteen mixed-sex pairs of calves (33 ± 3 days of age) were housed in pair pens for 1 week and then separated into adjacent single pens. In each of two successive weeks, in a cross-over design, the pairs of calves were given access to an arena (3.6 m × 7.10 m) for 45 min on two consecutive days followed by deprivation of arena access for 4 days or 0 days before being tested on the seventh day. On test days, health was scored for all calves. The bull calf of each pair was chosen as focal calf. The behaviour of the focal calf was recorded during the 20-min test. When deprived of arena access for 4 days, calves performed more parallel locomotor play than when deprived for 0 days (P < 0.001). Calves performed more frontal pushing in the second week of the study (P < 0.001). Calves performed less frontal pushing (social play) (P < 0.05) and tended to perform more individual locomotor play (P = 0.05) with increasing (worse) companion calf health score. The results suggest that the motivation for some, but not all, elements of calf play behaviour builds up over time. Thus, differentiating between elements of play may be important in the use of calf play behaviour to assess animal welfare. The association between the health of the companion calf and frontal pushing suggests that a healthier calf stimulates more social play.

The present observational study assessed the effect of deep straw bedding compared to cubicles during the dry period on lying-, feeding- and agonistic behaviour in Holstein cows. A total of 74 primi- (heifers) and multiparous cows were moved either onto deep straw bedding (DS) or into cubicle housing (CS) 4 wk before the expected calving date and were observed for 21 consecutive days. Throughout the 21 d lying time and the number of lying bouts were recorded using automated accelerometers while feeding time, the number of feeder visits and feed intake was recorded using automatic feed bins. All variables were averaged by week. The transition time from lying to standing was obtained by averaging five randomly selected standing up events on d 7 prior to the actual calving date while the number of failed standing attempts was obtained by following the cows standing up bouts throughout all of d 7 prior to the actual calving date. The frequency of agonistic behaviour was obtained from 7 h of continuous video recordings on d 21 and 7 (or 8) before the actual calving date. Lying time was not affected by treatment but DS cows had a higher number of lying bouts and a shorter transition time between lying and standing compared to CS cows. Primiparous cows had a higher number of lying bouts compared to multiparous cows. There were no treatment differences in feeding behaviour but multiparous cows spent more time feeding and increased their feed intake more across weeks compared to primiparous cows. Cows housed in DS initiated more agonistic behaviour compared to CS cows at d 7 (or 8) before calving while no difference between treatments was seen at 21 d before calving. These results suggest that deep straw bedding facilitates the transition between lying and standing but may increase agonistic behaviour.

We used spontaneous behaviours to assess response to dry-off involving abrupt dietary and milking frequency changes, followed by regrouping, after the last milking in 15 clinically healthy Holstein-Frisian cows kept outdoors. Moreover, we explored the potential of infrared thermography to detect eye temperature variations possibly induced by dry-off. On days − 1, 0, 1 and 2 relative to dry-off, we recorded whether cows vocalised during feed delivery; ate fresh feed within 5 min; and mean maximum eye temperature at approximately 1 h after feed delivery. On days 1 and 2, cows were more likely to eat fresh feed compared to days − 1 and 0. No difference in likelihood of vocalising was found. Compared to day − 1, eye temperature was substantially higher on days 0 and 2. Collectively, the results suggest that cows responded, both behaviourally and physiologically, to the abrupt dry-off management. The interpretation of the current findings deserves further investigation using larger sample sizes, more controlled environments and further behavioural, physiological, cognitive and clinical measures.

We used spontaneous behaviours to assess response to dry-off involving abrupt dietary and milking frequency changes, followed by regrouping, after the last milking in 15 clinically healthy Holstein-Frisian cows kept outdoors. Moreover, we explored the potential of infrared thermography to detect eye temperature variations possibly induced by dry-off. On days - 1, 0, 1 and 2 relative to dry-off, we recorded whether cows vocalised during feed delivery; ate fresh feed within 5 min; and mean maximum eye temperature at approximately 1 h after feed delivery. On days 1 and 2, cows were more likely to eat fresh feed compared to days - 1 and 0. No difference in likelihood of vocalising was found. Compared to day - 1, eye temperature was substantially higher on days 0 and 2. Collectively, the results suggest that cows responded, both behaviourally and physiologically, to the abrupt dry-off management. The interpretation of the current findings deserves further investigation using larger sample sizes, more controlled environments and further behavioural, physiological, cognitive and clinical measures.

Due to increasing public concern regarding separation of the dairy cow and calf within the first days after birth, alternative systems, where cows and calves stay in contact for an extended period, are receiving increasing interest from a broad array of researchers and other stakeholders. With more research in the area, there is a risk of inconsistencies emerging in the use of terminology. To create a better consensus in further discussions, the aim of this Research Reflection is to provide definitions and propose a common terminology for cow-calf contact in dairy production. We also suggest definitions for various systems allowing cow-calf contact and describe the distinct phases of cow-calf contact systems.

In this Research Reflection we describe a common standpoint on suitable methodology for controlled and observational studies in cow-calf contact systems in dairy production. Different methods to assess behaviour, health and production in cow-calf contact systems are outlined. Knowledge and experience from researchers working in this field supplement scientific literature whenever relevant. Specific methods including study design, early behaviour of cow and calf, social behaviour relevant to cow-calf contact systems, human-animal relationships and aspects related to management (milking, weaning and separation, health) are reviewed, and recommendations formed. We expect that this paper can contribute to a better understanding of the complexity of cow-calf contact systems and help to advance research in this area of dairy production.

The provision of straw to pigs kept in conventional pens with concrete floor improves animal welfare, but the effects of straw on pigs' performance are unclear. In two steps, we investigated the relationship between amount of straw provided to pigs and measures of performance in a set-up maintaining constant space allowance and controlled room temperature. From approximately 30- to 85-kg BW, pigs were housed in groups of 18 in pens (5.48 m × 2.48 m) with concrete floor (1/3 solid, 1/3 drained and 1/3 slatted). The pens were cleaned manually twice a week, and the designated amount of fresh uncut wheat straw was provided daily onto the solid part of the floor. In the first step, 48 pens were assigned to 10-, 500- or 1000-g straw per pig per day, while in the second step, 90 pens were assigned to 10-, 80-, 150-, 220-, 290-, 360-, 430- or 500-g straw per pig per day. Pigs were weighed at the start of the experimental period at approximately 30 kg and again at approximately 85-kg BW. The average daily gain increased 8.1 g (SEM 17) for every extra 100-g straw added daily (P < 0.001) resulting in 40 g higher average daily gain with 500 compared to 10-g straw per pig per day. The feed conversion ratio was not affected by the amount of straw provided, as the feed intake tended to be higher with increasing amounts of straw. Thus, between 10 and 500 g, the more straw provided, the higher the daily weight gain. As the nutritional value of straw is considered minimal, this result is likely due to improved gut health from the increasing amounts of straw ingested and increased feed intake due to increased stimulation of exploratory behaviour with increasing amounts of straw available, or a combination of these. The observed tendency for a higher feed intake supports this suggestion, but studies are needed to establish the impact of these two contributing factors.

In dairy production, mastitis is a major problem affecting animal welfare, productivity, and economy. Hospital pens are typically not used for cows with mastitis, except for severe cases involving recumbency. This field trial included 47 cows from three Danish herds followed for 8 d, of which days 1–5 involved the experimental housing. After day 5, all cows were kept with the lactating group. We examined lying behavior in dairy cows with naturally occurring, mild-moderate mastitis in hospital pens [single or group (depending on conditions on the farm), all with deep straw bedding] vs. sick cows kept in the group of healthy herd mates. Within a herd, every other cow fulfilling the inclusion criteria regarding mastitis was allocated to each of the two experimental treatments. Clinical data from involved cases were collected. No significant differences between housing treatments were found in the clinical variables or the daily lying time. During the period of experimental housing, cows kept in hospital pens showed a higher frequency of lying bouts compared with control cows. This difference did not persist after reintroduction to the lactating herd mates. These results suggest that aspects of lying behavior of dairy cows with mastitis are sensitive to the environment as the frequency of lying bouts differed between cows kept in hospital pens and cows kept in control treatment. More controlled studies are needed to examine underlying motivations and evaluate consequences in terms of animal welfare. For such studies, the inclusion of healthy cows for comparison will be valuable.

Today, we see a growing concern for the quality of life of nonhuman animals and an accompanying call for viable means of assessing how well animals thrive. Past research focused on minimizing negatives such as stress, while more recent endeavors strive to promote positives such as happiness. But what is animal happiness? Although often mentioned, the term lacks a clear definition. With recent advances in the study of animal emotion, current interest into positive rather than negative experiences, and the call for captive and domesticated animals to have good lives, the time is ripe to examine the concept of animal happiness. We draw from the human and animal literature to delineate a concept of animal happiness and propose how to assess it. We argue that animal happiness depends on how an individual feels generally—that is, a typical level of affect.

Under natural conditions, cows seek isolation and visual cover when calving becomes imminent, but the degree of visual cover necessary to provide an attractive calving site is not known. When calving indoors, preparturient cows are attracted to other cows' birth fluids, and this may influence their isolation seeking. Therefore, we aimed to investigate the effect of degree of visual cover of secluded areas and the location of birth fluids on dairy cows' calving site selection. One hundred twenty-four Danish Holstein cows were moved in groups of 8 to 12 to a group pen with access to 6 areas secluded by a barrier of either 3 m (wide barrier) or 1.5 m (narrow barrier). Sixty cows calved within 14 d of having been moved to the group pen and were included in the study. From 3 h before partition and until the cow and calf were removed from the pen within 3 h after parturition, the location and posture of cows were recorded, along with the location of where their birth fluids were discharged. In addition, location and posture of calves were recorded during and up to 3 h after calving. All data were collected from continuous video recordings. Continuous data for location and behavior were analyzed by mixed models, binary data were analyzed by chi-squared analysis, and location of calving relative to where the cow's own or alien birth fluids were discharged was analyzed using logistic regression models. Ten percent of the cows calved in a secluded area, with no effect of barrier width. However, before calving, cows with access to secluded areas with a wide barrier spent more time in a secluded area (22 vs. 7 min/3 h), and more cows were observed lying there (58 vs. 28%) than cows with access to secluded areas with a narrow barrier. Secluded areas with a wide barrier were used by more cows and calves during the first hour after calving (36 vs. 10%). Among the cows that entered a secluded area after calving, the latency to enter correlated with their calf's latency to enter, suggesting that the 2 sought seclusion together. Seventy-nine percent of the cows calved in proximity (within a 1.25-m radius) to their own or an alien cow's birth fluids. There was no effect of barrier width on the probability of calving in proximity to their own or alien birth fluids. However, the probability of calving in proximity to their own or alien birth fluids was higher than the probability of not doing so. The greater use of secluded areas with a 3-m-wide barrier before and after parturition suggests that these are preferred over areas with a 1.5-m-wide barrier. Only a few cows calved in a secluded area, and more research is needed to explore the environmental and social factors affecting parturient cows' use of designated calving areas. The majority of cows calved close to where their own or alien birth fluids were discharged, suggesting that cows are attracted to these before calving. This may have affected the use of secluded areas as calving sites.

Adequate time lying down is often considered an important aspect of dairy cow welfare. We examine what is known about cows' motivation to lie down and the consequences for health and other indicators of biological function when this behavior is thwarted. We review the environmental and animal-based factors that affect lying time in the context of animal welfare. Our objective is to review the research into the time that dairy cows spend lying down and to critically examine the evidence for the link with animal welfare. Cows can be highly motivated to lie down. They show rebound lying behavior after periods of forced standing and will sacrifice other activities, such as feeding, to lie down for an adequate amount of time. They will work, by pushing levers or weighted gates, to lie down and show possible indicators of frustration when lying behavior is thwarted. Some evidence suggests that risk of lameness is increased in environments that provide unfavorable conditions for cows to lie down and where cows are forced to stand. Lameness itself can result in longer lying times, whereas mastitis reduces it. Cow-based factors such as reproductive status, age, and milk production influence lying time, but the welfare implications of these differences are unknown. Lower lying times are reported in pasture-based systems, dry lots, and bedded packs (9 h/d) compared with tiestalls and freestalls (10 to 12 h/d) in cross-farm research. Unfavorable conditions, including too few lying stalls for the number of cows, hard or wet lying surfaces, inadequate bedding, stalls that are too small or poorly designed, heat, and rain all reduce lying time. Time constraints, such as feeding or milking, can influence lying time. However, more information is needed about the implications of mediating factors such as the effect of the standing surface (concrete, pasture, or other surfaces) and cow behavior while standing (e.g., being restrained, walking, grazing) to understand the effect of low lying times on animal welfare. Many factors contribute to the difficulty of finding a valid threshold for daily lying time to use in the assessment of animal welfare. Although higher lying times often correspond with cow comfort, and lower lying times are seen in unfavorable conditions, exceptions occur, namely when cows lie down for longer because of disease or when they spend more time standing because of estrus or parturition, or to engage in other behaviors. In conclusion, lying behavior is important to dairy cattle, but caution and a full understanding of the context and the character of the animals in question is needed before drawing firm conclusions about animal welfare from measures of lying time.

In modern calving facilities, dairy cows either calve in a group pen or are moved to a separate individual pen when calving is imminent. In practice, cows are often moved too close to calving, which poses a health risk to cow and calf. Thus, a need exists for new calving facility designs and management practices that better align with the motivations of the cow. This study examined dairy cow preferences for individual calving pens by offering 3 different levels of isolation (tall and narrow, low and wide, and tall and wide) by analyzing the association between precalving behavior, choice of degree of isolation, and the progress of calving. The hypotheses were that cows would prefer the highest level of isolation when giving birth, and that calving in a high level of isolation would be associated with less restlessness and a shorter calving duration. Contrary to these hypotheses, no specific preference between degrees of isolation or difference in calving behavior in the different calving pens was found. However, cows experiencing a longer calving duration chose to calve in the most secluded calving pen (tall and wide). These results cannot determine cause and effect, but may suggest that interactions between motivation for isolation seeking and calving behavior exist.

Drying-off is one important management step in commercial dairy farms and consists of ceasing milk production artificially at a specific point in time, generally 2 months before the next calving. Drying-off typically comprises dietary changes as well as gradual or abrupt changes in daily milking frequency, which may challenge the welfare of high-yielding cows. This study investigated the isolated and combined effects of different feed energy densities (normal lactation diet versus energy-reduced diet, both offered ad libitum) and daily milking frequencies (twice versus once) on the feeding motivation of dairy cows on two separate days prior to dry-off (i.e. the day of last milking) using a push-gate feeder. During both days, cows on the energy-reduced diet pushed more than five times more weight to earn the final feed reward and were nearly ten times faster to feed on the first reward than cows on the normal lactation diet. Illustrating the importance of developing more animal welfare-friendly dry-off management, these results illustrate that cows show signs of hunger prior to dry-off when provided a diet with reduced energy density, although offered for ad libitum intake.

The present study investigated the effect of straw yard housing during the dry period and 2 d of additional maternity pen housing postcalving on lying and feeding behavior and calving difficulty in Holstein dairy cows. In this study, 122 multiparous cows were moved to either a straw yard or into freestall housing 4 wk before their expected calving date. Cows that had been housed in straw yards stayed in the maternity pen for an additional 2 d after their calving day, but cows that had been housed in freestalls were moved to the general lactation group the morning after calving. Lying time, lying bouts, feeding time, number of feeder visits, feed intake, feeding rate, and assisted calvings were recorded. Observations were divided into 2 periods: precalving (the 4-wk dry period before calving) and postcalving (the day of calving and the 2 d after). During the precalving period, cows housed in straw yards showed a higher number of lying bouts but no difference in lying time compared with cows housed in freestalls. Cows that were housed longer in the straw-bedded maternity pen postcalving spent more time lying during the 2 d postcalving and had a higher number of lying bouts on the day of calving than cows moved to the freestall area on the day postcalving. Additionally, cows that were housed longer in the maternity pen had a slower feeding rate and longer total feeding time during the 2 d after calving than cows with a shorter stay in the maternity pen. We found no difference in the number of assisted calvings. This study suggests that straw yard housing during the dry period may facilitate the transition between standing and lying. Furthermore, the extended stay in the maternity pen postcalving increased lying time, the number of lying bouts, and feeding time, but decreased feeding rate compared with cows that were moved to the general lactation group on the day postcalving. These results suggest potential recovery benefits with an extended stay in a maternity pen postcalving. However, further studies are needed to separate the effects of housing in the dry period and the effects of an extended housing in individual maternity pens.

Dairy cows typically have their milk production artificially ceased around 60 d before calving. This process, termed drying-off, often comprises changes of diet and milking frequency. Evidence suggest drying-off challenges the welfare of high-yielding cows. For instance, a nutrient-reduced diet can impair energy balance and cause hunger even when offered ad libitum. This ongoing work proposes a feed frustration test, inducing motivation conflict, to assess isolated and combined effects of daily milking frequency (twice; once) and diet (lactation diet or energy-reduced lactation diet with forage:concentrate ratios of 51:49 and 65:35, respectively, ad libitum) on the degree of hunger. Biweekly, groups of 2-6 healthy loose-housed Holstein cows were allocated to one of four treatments from 7 d (D-7) prior to the last day of milking (DO). In total, 92 cows (mean±SD) 754±80 kg in body weight and yielding 25±6 kg of milk/d were included in this study. From D-7 to DO, each cow had access to one computerised feed bin providing access to the respective diet. Tests were conducted on D-6 and D-1. First, barn staff remotely locked the feed bins and initiated the second daily feed delivery (at approx. 10.30 h). Duration of feed delivery ranged from 15-76 min and constituted the routine phase (phase A). When the feed delivery ended, all feed bins were remotely unlocked, except the experimental ones, which remained locked for additional 35 min, constituting the extra feed deprivation phase (phase B). During B, experimental cows could hear other feed bins opening and see cows in neighbouring pens feeding. During both phases, an experimenter standing in front of the feed bins approx. 4 m away recorded attempts to feed (i.e. focal cow puts the head over any locked feed bin, placing her collar past the feed bin gate) and high-pitched vocalisations (≥3 s). Following unlocking of experimental feed bins the latency to feed was recorded for 5 min. Data analyses were performed in R for each day separately. Attempts to feed in A were treated as counts/min due to a wide time range, while attempts to feed in B were treated as counts. Due to a low representation of vocalisations in the data set, this variable was analysed as binary (yes; no). Latency to feed was also analysed as binary (yes; no). All variables were analysed using GLMM including diet and milking frequency as fixed effects and block as random effect. On D-6, no significant differences were found between treatments, except the odds of vocalising being higher in cows on energy-reduced diet than normal diet [OR (95% CI)] [A: 5.4 (2.1 to 15.5), χ2=11.4, DF=1, P

Pigs are highly motivated to perform exploratory behaviour and must have permanent access to manipulable material according to the EU Directive 2008/120/EC. A commonly used type of manipulable material is wooden beams. The beams are placed in vertical racks and touch the floor. According to Danish implementation of the directive, at least two wooden beams should be provided to a pen of 10–18 finisher pigs. However, it has not been investigated whether access to a higher number of wooden beams benefits pigs by stimulating more oral manipulation. The aim of the current study was to investigate the effect of increasing the number of wooden beams from two to four in pens of 18 finisher pigs. Response variables were manipulation of wooden beams including bout frequency, bout duration, hourly duration, proportion of pigs manipulating within the hour and proportion of pigs manipulating simultaneously. Behaviour was observed from video on a fixed weekday in weeks 2, 4, 6 and 8 of the finisher period. The effects of number of beams, beam location, observation week and pigs’ introduction weight were analysed. Increasing the number of beams from two to four increased the bout frequency, average bout duration, hourly duration of manipulating the beams and the proportion of pigs manipulating within the hour; however, it did not increase the proportion of pigs manipulating simultaneously. Only bout frequency decreased with time, and this decrease was seen later in pens with four beams. Thus, four compared to two beams increased the exploratory behaviour of the pigs, the number of pigs engaged in the manipulation and kept the pigs interested in the beams for longer. In pens with four beams, beam location also affected beam manipulation with a higher bout frequency on beams located on the opposite side of the pen as the feeder. Pigs’ introduction weight did not affect pigs’ manipulation of the beams. The results suggest that the pigs benefit from being provided with a higher number of beams than currently demanded by the Danish implementation of the EU Directive.

Behaviour is commonly used to detect sickness in animals, but the impact of sickness on lying and maternal behaviours around parturition is not well understood. The objective was to assess the effects of sickness on the lying and grooming behaviours of dairy cows in the first 24 h after giving birth. Cows were categorized as &lsquo, sick&rsquo, (n = 8) if they had at least one rectal temperature &ge, 39.1 &deg, C and one clinical sign of illness (mastitis, pneumonia or an unknown infection) within 24 h of calving. These cows were match-paired for parity with cows that had no rectal temperature &ge, C and no clinical signs of illness up to 3 d after calving (n = 8, &lsquo, not sick&rsquo, ). The duration and latency of cow behaviours (standing, lying, lying bouts, lying close to calf, and grooming of the calf) and calf behaviours (standing and lying) were recorded for 24 h post-partum. We found no differences in the behaviour of sick and not sick cows and their calves post-calving, except that sick cows took longer to lie down near their calf after calving compared to those without illness. Cows may be more motivated to groom and spend time with their calf than to express sickness behaviours immediately after giving birth.

Rosé veal farmers using TMR want to feed calves high-energy TMR already from weaning, either alone or in combination with a pelleted concentrate. The objective was to test how weaned dairy bull calves perform from 10 to 18 wk of age when offered either free choice between pelleted concentrate and TMR (FREE) or only TMR (TMR). The experiment used 32 Holstein bull calves purchased at 11 ± 1 d of age and 47 ± 1 kg LW (Mean±SE). From 2 wk until weaning at 8 wk of age, calves were offered a total of 224 L milk replacer (23% CP, 19% fat). From 2 to 10 wk of age, calves had free access to a pelleted concentrate (20% CP) and chopped hay (14% CP). At 10 wk (97 ± 2 kg LW) calves were assigned to FREE or TMR. The pelleted concentrate and the TMR had (kg DM basis) similar NE (8.8 MJ), CP (213 g), NDF (195 g), and starch contents (328 g), but varied in DM (88 vs. 70%), feed composition, and physical form. The TMR included 31% first-cut grass silage. Hay comprised 2.5% of NEI for both FREE and TMR. TMR intake comprised 61% and concentrate pellet intake 36.5% of NEI for FREE calves. LW at 18 wk was 178 and 173 kg (n.s.) and ADG from 10 to 18 wk was 1.46 and 1.36 kg/d (P

Access to drinking water is essential for animal welfare, but it is unclear if temporary water restriction during the night represents a welfare problem. The aim of the present study was to investigate the effect of various durations of nightly restriction of water on thirst in loose housed lactating sows from day 10 to 28 of lactation. A total of 48 sows were deprived of water for either 0 h (n = 12; control), 3 h (n = 12; 0500 to 0800 h), 6 h (n = 12; 0200 to 0800 h) or 12 h (n = 12; 2000 to 0800 h). Control sows consumed 22% of their water intake during the night (2000 to 0800 h), whereas water consumption during this time was reduced to 13%, 7% and 0% in sows restricted for 3, 6 and 12 h. With increased duration of nightly water restriction a reduced latency to drink (26.8, 18.0, 5.3 and 6.7 min for 0, 3, 6 and 12 h sows; P

A widespread management practice in commercial dairy farms is to stop milk production artificially commonly 2 months before expected calving, in a process termed drying-off. Dry-off management normally encompasses dietary changes as well as abrupt or gradual changes in daily milking frequency, which can compromise the welfare of modern cows. This study investigated the effects of various dry-off strategies comprising feed energy density (normal lactation diet versus energy-reduced diet, both fed for ad libitum intake) and daily milking frequency (twice versus once) from seven days prior to the day of last milking (i.e. dry-off day - D0) on the feeding motivation, feed-related attention bias and visual lateralisation towards feed of dairy cows. During two separate days (D-5 and D-2) prior to dry-off, cows on the energy-reduced diet started feeding much earlier than cows on the normal diet. No effect of daily milking frequency or interaction with feed energy density on feeding motivation were found. The increased feeding motivation induced by a low energy feed did not reflect changes in neither feed-related attention bias nor visual lateralisation towards feed. In agreement with the literature, the majority of the cows showed right eye dominance towards a feed source when feeding, corresponding to the left-brain hemisphere. Our study supports the suggestion that cows offered low energy diets show signs of hunger prior to dry-off, even when fed for ad libitum intake. The current study sheds light on the relevance of developing more animal-friendly dry-off management, as well as the importance of developing existing feed-related attention bias tests for nonhuman animals.