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10 results on '"Marei, H. E."'

1. Exome sequencing of glioblastoma-derived cancer stem cells reveals rare clinically relevant frameshift deletion in MLLT1 gene

Author

Marei, H. E., Althani, A., Afifi, N., Hasan, A., Caceci, T., Felsani, A., Tringali, Giuseppe, Cifola, I., Pozzoli, Giacomo, Cenciarelli, C., Tringali G. (ORCID:0000-0002-1030-9429), Pozzoli G. (ORCID:0000-0003-0398-7026), Marei, H. E., Althani, A., Afifi, N., Hasan, A., Caceci, T., Felsani, A., Tringali, Giuseppe, Cifola, I., Pozzoli, Giacomo, Cenciarelli, C., Tringali G. (ORCID:0000-0002-1030-9429), and Pozzoli G. (ORCID:0000-0003-0398-7026)

Abstract

Background: Glioblastoma multiforme (GBM) is a heterogeneous CNS neoplasm which causes significant morbidity and mortality. One reason for the poor prognostic outcome of GBM is attributed to the presence of cancer stem cells (CSC) which confer resistance against standard chemo- and radiotherapeutics modalities. Two types of GBM-associated CSC were isolated from the same patient: tumor core- (c-CSC) and peritumor tissue-derived cancer stem cells (p-CSC). Our experiments are focused on glioblastoma–IDH-wild type, and no disease-defining alterations were present in histone, BRAF or other genes. Methods: In the present study, potential differences in genetic variants between c-CSC versus p-CSC derived from four GBM patients were investigated with the aims of (1) comparing the exome sequences between all the c-CSC or p-CSC to identify the common variants; (2) identifying the variants affecting the function of genes known to be involved in cancer origin and development. Results: By comparative analyses, we identified common gene single nucleotide variants (SNV) in all GBM c-CSC and p-CSC, a potentially deleterious variant was a frameshift deletion at Gln461fs in the MLLT1 gene, that was encountered only in p-CSC samples with different allelic frequency. Conclusions: We discovered a potentially harmful frameshift deletion at Gln461fs in the MLLT1 gene. Further investigation is required to confirm the presence of the identified mutations in patient tissue samples, as well as the significance of the frameshift mutation in the MLLT1 gene on GBM biology and response to therapy based on genomic functional experiments.

Published
2022

2. EGFR GLIOBLASTOMA STEM CELLS TARGETING BY CD16158V CHIMERIC RECEPTOR T CELLS AND CETUXIMAB

Author

Cenciarelli, C., primary, Caratelli, S., additional, Arriga, R., additional, Lanzilli, G., additional, Stabile, S., additional, Ottaviani, A., additional, Sconocchia, T., additional, Spagnoli, G. C., additional, Venditti, A., additional, Iezzi, G., additional, Roselli, M., additional, Lauro, D., additional, Marei, H. E., additional, and Sconocchia, G., additional

Published
2021
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3. p53 signaling in cancer progression and therapy

Author

Marei, H. E., Althani, A., Afifi, N., Hasan, A., Caceci, T., Pozzoli, Giacomo, Morrione, A., Giordano, A., Cenciarelli, C., Pozzoli G. (ORCID:0000-0003-0398-7026), Marei, H. E., Althani, A., Afifi, N., Hasan, A., Caceci, T., Pozzoli, Giacomo, Morrione, A., Giordano, A., Cenciarelli, C., and Pozzoli G. (ORCID:0000-0003-0398-7026)

Abstract

The p53 protein is a transcription factor known as the "guardian of the genome" because of its critical function in preserving genomic integrity. The TP53 gene is mutated in approximately half of all human malignancies, including those of the breast, colon, lung, liver, prostate, bladder, and skin. When DNA damage occurs, the TP53 gene on human chromosome 17 stops the cell cycle. If p53 protein is mutated, the cell cycle is unrestricted and the damaged DNA is replicated, resulting in uncontrolled cell proliferation and cancer tumours. Tumor-associated p53 mutations are usually associated with phenotypes distinct from those caused by the loss of the tumor-suppressing function exerted by wild-type p53protein. Many of these mutant p53 proteins have oncogenic characteristics, and therefore modulate the ability of cancer cells to proliferate, escape apoptosis, invade and metastasize. Because p53 deficiency is so common in human cancer, this protein is an excellent option for cancer treatment. In this review, we will discuss some of the molecular pathways by which mutant p53 proteins might perform their oncogenic activities, as well as prospective treatment methods based on restoring tumor suppressive p53 functions.

Published
2021

4. Erratum: Antimicrobials: A global alliance for optimizing their rational use in intra-abdominal infections (AGORA). [World J Emerg Surg. 11, (2016) (33)] DOI: 10.1186/s13017-016-0089-y

Author

Sartelli, M., Weber, D. G., Ruppé, E., Bassetti, M., Wright, B. J., Ansaloni, L., Catena, F., Coccolini, F., Abu-Zidan, F. M., Coimbra, R., Moore, E. E., Moore, F. A., Maier, R. V., De Waele, J. J., Kirkpatrick, A. W., Griffiths, E. A., Eckmann, C., Brink, A. J., Mazuski, J. E., May, A. K., Sawyer, R. G., Mertz, D., Montravers, P., Kumar, A., Roberts, J. A., Vincent, J. L., Watkins, R. R., Lowman, W., Spellberg, B., Abbott, I. J., Adesunkanmi, A. K., Al-Dahir, S., Al-Hasan, M. N., Agresta, F., Althani, A. A., Ansari, S., Ansumana, R., Augustin, G., Bala, M., Balogh, Z. J., Baraket, O., Bhangu, A., Beltrán, M. A., Bernhard, M., Biffl, W. L., Boermeester, M. A., Brecher, S. M., Cherry-Bukowiec, J. R., Buyne, O. R., Cainzos, M. A., Cairns, K. A., Camacho-Ortiz, A., Chandy, S. J., Che Jusoh, A., Chichom-Mefire, A., Colijn, C., Corcione, F., Cui, Y., Curcio, D., Delibegovic, S., Demetrashvili, Z., De Simone, B., Dhingra, S., Diaz, J. J., Di Carlo, I., Dillip, A., Di Saverio, S., Doyle, M. P., Dorj, G., Dogjani, A., Dupont, H., Eachempati, S. R., Enani, M. A., Egiev, V. N., Elmangory, M. M., Ferrada, P., Fitchett, J. R., Fraga, G. P., Guessennd, N., Giamarellou, H., Ghnnam, W., Gkiokas, G., Goldberg, S. R., Gomes, C. A., Gomi, H., Guzmán-Blanco, M., Haque, M., Hansen, S., Hecker, A., Heizmann, W. R., Herzog, T., Hodonou, A. M., Hong, S. K., Kafka-Ritsch, R., Kaplan, L. J., Kapoor, G., Karamarkovic, A., Kees, M. G., Kenig, J., Kiguba, R., Kim, P. K., Kluger, Y., Khokha, V., Koike, K., Kok, K. Y., Kong, V., Knox, M. C., Inaba, K., Isik, A., Iskandar, K., Ivatury, R. R., Labbate, M., Labricciosa, F. M., Laterre, P. F., Latifi, R., Lee, J. G., Lee, Y. R., Leone, M., Leppaniemi, A., Li, Y., Liang, S. Y., Loho, T., Maegele, M., Malama, S., Marei, H. E., Martin-Loeches, I., Marwah, S., Massele, A., Mcfarlane, M., Melo, R. B., Negoi, I., Nicolau, D. P., Nord, C. E., Ofori-Asenso, R., Omari, A. H., Ordonez, C. A., Ouadii, M., Pereira Júnior, G. A., Piazza, D., Pupelis, G., Rawson, T. M., Rems, M., Rizoli, S., Rocha, C., Sakakushev, B., Sanchez-Garcia, M., Sato, N., Segovia Lohse, H. A., Sganga, G., Siribumrungwong, B., Shelat, V. G., Soreide, K., Soto, R., Talving, P., Tilsed, J. V., Timsit, J. F., Trueba, G., Trung, N. T., Ulrych, J., van Goor, H., Vereczkei, A., Vohra, R. S., Wani, I., Uhl, W., Xiao, Y., Yuan, K. C., Zachariah, S. K., Zahar, J. R., Zakrison, T. L., Corcione, A., Melotti, R. M., Viscoli, C., Viale, P., Sartelli, M., Weber, D. G., Ruppé, E., Bassetti, M., Wright, B. J., Ansaloni, L., Catena, F., Coccolini, F., Abu-Zidan, F. M., Coimbra, R., Moore, E. E., Moore, F. A., Maier, R. V., De Waele, J. J., Kirkpatrick, A. W., Griffiths, E. A., Eckmann, C., Brink, A. J., Mazuski, J. E., May, A. K., Sawyer, R. G., Mertz, D., Montravers, P., Kumar, A., Roberts, J. A., Vincent, J. L., Watkins, R. R., Lowman, W., Spellberg, B., Abbott, I. J., Adesunkanmi, A. K., Al-Dahir, S., Al-Hasan, M. N., Agresta, F., Althani, A. A., Ansari, S., Ansumana, R., Augustin, G., Bala, M., Balogh, Z. J., Baraket, O., Bhangu, A., Beltrán, M. A., Bernhard, M., Biffl, W. L., Boermeester, M. A., Brecher, S. M., Cherry-Bukowiec, J. R., Buyne, O. R., Cainzos, M. A., Cairns, K. A., Camacho-Ortiz, A., Chandy, S. J., Che Jusoh, A., Chichom-Mefire, A., Colijn, C., Corcione, F., Cui, Y., Curcio, D., Delibegovic, S., Demetrashvili, Z., De Simone, B., Dhingra, S., Diaz, J. J., Di Carlo, I., Dillip, A., Di Saverio, S., Doyle, M. P., Dorj, G., Dogjani, A., Dupont, H., Eachempati, S. R., Enani, M. A., Egiev, V. N., Elmangory, M. M., Ferrada, P., Fitchett, J. R., Fraga, G. P., Guessennd, N., Giamarellou, H., Ghnnam, W., Gkiokas, G., Goldberg, S. R., Gomes, C. A., Gomi, H., Guzmán-Blanco, M., Haque, M., Hansen, S., Hecker, A., Heizmann, W. R., Herzog, T., Hodonou, A. M., Hong, S. K., Kafka-Ritsch, R., Kaplan, L. J., Kapoor, G., Karamarkovic, A., Kees, M. G., Kenig, J., Kiguba, R., Kim, P. K., Kluger, Y., Khokha, V., Koike, K., Kok, K. Y., Kong, V., Knox, M. C., Inaba, K., Isik, A., Iskandar, K., Ivatury, R. R., Labbate, M., Labricciosa, F. M., Laterre, P. F., Latifi, R., Lee, J. G., Lee, Y. R., Leone, M., Leppaniemi, A., Li, Y., Liang, S. Y., Loho, T., Maegele, M., Malama, S., Marei, H. E., Martin-Loeches, I., Marwah, S., Massele, A., Mcfarlane, M., Melo, R. B., Negoi, I., Nicolau, D. P., Nord, C. E., Ofori-Asenso, R., Omari, A. H., Ordonez, C. A., Ouadii, M., Pereira Júnior, G. A., Piazza, D., Pupelis, G., Rawson, T. M., Rems, M., Rizoli, S., Rocha, C., Sakakushev, B., Sanchez-Garcia, M., Sato, N., Segovia Lohse, H. A., Sganga, G., Siribumrungwong, B., Shelat, V. G., Soreide, K., Soto, R., Talving, P., Tilsed, J. V., Timsit, J. F., Trueba, G., Trung, N. T., Ulrych, J., van Goor, H., Vereczkei, A., Vohra, R. S., Wani, I., Uhl, W., Xiao, Y., Yuan, K. C., Zachariah, S. K., Zahar, J. R., Zakrison, T. L., Corcione, A., Melotti, R. M., Viscoli, C., and Viale, P.

Subjects
Settore MED/18 - CHIRURGIA GENERALE, Surgery, Emergency Medicine
Published
2017

5. Antimicrobials: a global alliance for optimizing their rational use in intra-abdominal infections (AGORA) (vol 11, 33, 2016)

Author

Sartelli, M., Weber, D. G., Ruppe, E., Bassetti, M., Wright, B. J., Ansaloni, L., Catena, F., Coccolini, F., Abu-Zidan, F. M., Coimbra, R., Moore, E. E., Moore, F. A., Maier, R. V., De Waele, J. J., Kirkpatrick, A. W., Griffiths, E. A., Eckmann, C., Brink, A. J., Mazuski, J. E., May, A. K., Sawyer, R. G., Mertz, D., Montravers, P., Kumar, A., Roberts, J. A., Vincent, L., Watkins, R. R., Lowman, W., Spellberg, B., Abbott, I. J., Adesunkanmi, A. K., Al-Dahir, S., Al-Hasan, M. N., Agresta, F., Althani, A. A., Ansari, S., Ansumana, R., Augustin, G., Bala, M., Balogh, Z. J., Baraket, O., Bhangu, A., Beltrán, Anna Maria, Bernhard, M., Biffl, W. L., Boermeester, M. A., Brecher, S. M., Cherry-Bukowiec, J. R., Buyne, O. R., Cainzos, M. A., Cairns, K. A., Camacho-Ortiz, A., Chandy, S. J., Jusoh, A. Che, Chichom-Mefire, A., Colijn, C., Corcione, F., Cui, Y., Curcio, D., Delibegovic, S., Demetrashvili, Z., De Simone, B., Dhingra, S., Diaz, J. J., Di Carlo, I., Dillip, A., Di Saverio, S., Doyle, M. P., Dorj, G., Dogjani, A., Dupont, H., Eachempati, S. R., Enani, M. A., Egiev, V. N., Elmangory, M. M., Ferrada, P., Fitchett, J. R., Fraga, G. P., Guessennd, N., Giamarellou, H., Ghnnam, W., Gkiokas, G., Goldberg, S. R., Gomes, C. A., Gomi, H., Guzman-Blanco, M., Haque, M., Hansen, S., Hecker, A., Heizmann, W. R., Herzog, T., Hodonou, A. M., Hong, S. K., Kafka-Ritsch, R., Kaplan, L. J., Kapoor, G., Karamarkovic, A., Kees, M. G., Kenig, J., Kiguba, R., Kim, P. K., Kluger, Y., Khokha, V., Koike, K., Kok, K. Y., Kong, V., Knox, M. C., Inaba, K., Isik, A., Iskandar, K., Ivatury, R. R., Labbate, M., Labricciosa, F. M., Laterre, P. F., Latifi, R., Lee, J. G., Lee, Y. R., Leone, M., Leppaniemi, A., Li, Y., Liang, S. Y., Loho, T., Maegele, M., Malama, S., Marei, H. E., Martin-Loeches, I., Marwah, S., Massele, A., McFarlane, M., Melo, R. B., Negoi, I., Nicolau, D. P., Nord, C. E., Ofori-Asenso, R., Omari, A. H., Ordonez, C. A., Ouadii, M., Pereira Junior, G. A., Piazza, D., Pupelis, G., Rawson, T. M., Rems, M., Rizoli, S., Rocha, C., Sakakushev, B., Sanchez-Garcia, M., Sato, N., Segovia Lohse, H. A., Sganga, G., Siribumrungwong, B., Shelat, V. G., Soreide, K., Soto, R., Talving, P., Tilsed, J. V., Timsit, J. F., Trueba, G., Trung, N. T., Ulrych, J., Van Goor, H., Vereczkei, A., Vohra, R. S., Wani, I., Uhl, W., Xiao, Y., Yuan, K. C., Zachariah, S. K., Zahar, J. R., Zakrison, T. L., Corcione, A., Melotti, R. M., Viscoli, C., Viale, P., Universita 'La Sapienza' Roma (Istituto CNR), Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome] (UNIROMA), Universidade de Aveiro, Laboratoire matériaux et microélectronique de Provence (L2MP), Université Paul Cézanne - Aix-Marseille 3-Université de Provence - Aix-Marseille 1-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur de Côte d'Ivoire, Réseau International des Instituts Pasteur (RIIP), National Defence University of Malaysia [Kuala Lumpur], Max Planck Institute for the Physics of Complex Systems (MPI-PKS), Max-Planck-Gesellschaft, Center for Plant Molecular Biology, Plant Physiology, and Biophysical Chemistry, University of Tübingen, Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR48, Institut des sciences biologiques (INSB-CNRS)-Institut des sciences biologiques (INSB-CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de l'Accélérateur Linéaire (LAL), Université Paris-Sud - Paris 11 (UP11)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Dipartimento di Fisica 'Giuseppe Occhialini' = Department of Physics 'Giuseppe Occhialini' [Milano-Bicocca], Università degli Studi di Milano-Bicocca = University of Milano-Bicocca (UNIMIB), Atmospheric and Environmental Research, Inc. (AER), Zhejiang University, Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome], INSB-INSB-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Paris-Sud - Paris 11 (UP11), Università degli Studi di Milano-Bicocca [Milano] (UNIMIB), and COMBE, Isabelle

Subjects
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Carbapenems, Antimicrobial Resistance, Invasive Candidiasis, Methicillin Resistant Staphylococcus Aureus, Tigecycline, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

International audience; Intra-abdominal infections (IAI) are an important cause of morbidity and are frequently associated with poor prognosis, particularly in high-risk patients.The cornerstones in the management of complicated IAIs are timely effective source control with appropriate antimicrobial therapy. Empiric antimicrobial therapy is important in the management of intra-abdominal infections and must be broad enough to cover all likely organisms because inappropriate initial antimicrobial therapy is associated with poor patient outcomes and the development of bacterial resistance.The overuse of antimicrobials is widely accepted as a major driver of some emerging infections (such as C. difficile), the selection of resistant pathogens in individual patients, and for the continued development of antimicrobial resistance globally. The growing emergence of multi-drug resistant organisms and the limited development of new agents available to counteract them have caused an impending crisis with alarming implications, especially with regards to Gram-negative bacteria.An international task force from 79 different countries has joined this project by sharing a document on the rational use of antimicrobials for patients with IAIs. The project has been termed AGORA (Antimicrobials: A Global Alliance for Optimizing their Rational Use in Intra-Abdominal Infections). The authors hope that AGORA, involving many of the world's leading experts, can actively raise awareness in health workers and can improve prescribing behavior in treating IAIs.

Published
2017
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6. Erratum to: Antimicrobials: a global alliance for optimizing their rational use in intra-abdominal infections (AGORA)

Author

Sartelli, M., primary, Weber, D. G., additional, Ruppé, E., additional, Bassetti, M., additional, Wright, B. J., additional, Ansaloni, L., additional, Catena, F., additional, Coccolini, F., additional, Abu-Zidan, F. M., additional, Coimbra, R., additional, Moore, E. E., additional, Moore, F. A., additional, Maier, R. V., additional, De Waele, J. J., additional, Kirkpatrick, A. W., additional, Griffiths, E. A., additional, Eckmann, C., additional, Brink, A. J., additional, Mazuski, J. E., additional, May, A. K., additional, Sawyer, R. G., additional, Mertz, D., additional, Montravers, P., additional, Kumar, A., additional, Roberts, J. A., additional, Vincent, J. L., additional, Watkins, R. R., additional, Lowman, W., additional, Spellberg, B., additional, Abbott, I. J., additional, Adesunkanmi, A. K., additional, Al-Dahir, S., additional, Al-Hasan, M. N., additional, Agresta, F., additional, Althani, A. A., additional, Ansari, S., additional, Ansumana, R., additional, Augustin, G., additional, Bala, M., additional, Balogh, Z. J., additional, Baraket, O., additional, Bhangu, A., additional, Beltrán, M. A., additional, Bernhard, M., additional, Biffl, W. L., additional, Boermeester, M. A., additional, Brecher, S. M., additional, Cherry-Bukowiec, J. R., additional, Buyne, O. R., additional, Cainzos, M. A., additional, Cairns, K. A, additional, Camacho-Ortiz, A., additional, Chandy, S. J., additional, Che Jusoh, A., additional, Chichom-Mefire, A., additional, Colijn, C., additional, Corcione, F., additional, Cui, Y., additional, Curcio, D., additional, Delibegovic, S., additional, Demetrashvili, Z., additional, De Simone, B., additional, Dhingra, S., additional, Diaz, J. J., additional, Di Carlo, I., additional, Dillip, A., additional, Di Saverio, S., additional, Doyle, M. P., additional, Dorj, G., additional, Dogjani, A., additional, Dupont, H., additional, Eachempati, S. R., additional, Enani, M. A., additional, Egiev, V. N., additional, Elmangory, M. M., additional, Ferrada, P., additional, Fitchett, J. R., additional, Fraga, G. P., additional, Guessennd, N., additional, Giamarellou, H., additional, Ghnnam, W., additional, Gkiokas, G., additional, Goldberg, S. R., additional, Gomes, C. A., additional, Gomi, H., additional, Guzmán-Blanco, M., additional, Haque, M., additional, Hansen, S., additional, Hecker, A., additional, Heizmann, W. R., additional, Herzog, T., additional, Hodonou, A. M., additional, Hong, S. K., additional, Kafka-Ritsch, R., additional, Kaplan, L. J., additional, Kapoor, G., additional, Karamarkovic, A., additional, Kees, M. G., additional, Kenig, J., additional, Kiguba, R., additional, Kim, P. K., additional, Kluger, Y., additional, Khokha, V., additional, Koike, K., additional, Kok, K. Y., additional, Kong, V., additional, Knox, M. C., additional, Inaba, K., additional, Isik, A., additional, Iskandar, K., additional, Ivatury, R. R., additional, Labbate, M., additional, Labricciosa, F. M., additional, Laterre, P. F., additional, Latifi, R., additional, Lee, J. G., additional, Lee, Y. R., additional, Leone, M., additional, Leppaniemi, A., additional, Li, Y., additional, Liang, S. Y., additional, Loho, T., additional, Maegele, M., additional, Malama, S., additional, Marei, H. E., additional, Martin-Loeches, I., additional, Marwah, S., additional, Massele, A., additional, McFarlane, M., additional, Melo, R. B., additional, Negoi, I., additional, Nicolau, D. P., additional, Nord, C. E., additional, Ofori-Asenso, R., additional, Omari, A. H., additional, Ordonez, C. A., additional, Ouadii, M., additional, Pereira Júnior, G. A., additional, Piazza, D., additional, Pupelis, G., additional, Rawson, T. M., additional, Rems, M., additional, Rizoli, S., additional, Rocha, C., additional, Sakakushev, B., additional, Sanchez-Garcia, M., additional, Sato, N., additional, Segovia Lohse, H. A., additional, Sganga, G., additional, Siribumrungwong, B., additional, Shelat, V. G., additional, Soreide, K., additional, Soto, R., additional, Talving, P., additional, Tilsed, J. V., additional, Timsit, J. F., additional, Trueba, G., additional, Trung, N. T., additional, Ulrych, J., additional, van Goor, H., additional, Vereczkei, A., additional, Vohra, R. S., additional, Wani, I., additional, Uhl, W., additional, Xiao, Y., additional, Yuan, K. C., additional, Zachariah, S. K., additional, Zahar, J. R., additional, Zakrison, T. L., additional, Corcione, A., additional, Melotti, R. M., additional, Viscoli, C., additional, and Viale, P., additional

Published
2017
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9. Morphological and glycohistochemical studies on the epididymal region of the Sudani duck (Cairina moschata).

Author

Abd-Elmaksoud A, Sayed-Ahmed A, Mohamed SE, Mohamed K, and Marei HE

Subjects
Animals, Epididymis ultrastructure, Histocytochemistry veterinary, Male, Microscopy, Fluorescence veterinary, Carbohydrates chemistry, Ducks anatomy & histology, Ducks metabolism, Epididymis anatomy & histology, Epididymis metabolism, Lectins chemistry
Abstract

In this study, the epididymal region of the Sudani duck was investigated using histological and lectin histochemical methods. Morphologically, the epididymal region of the Sudani duck is composed of extratesticular rete testis, proximal and distal efferent ductules, a short connecting duct, and epididymal ducts. Morphometric analysis of the epididymal region of Sudani duck revealed that the efferent ductules predominate in relation to the epididymal ducts. The distribution of sugar moieties within the epididymal region of the Sudani duck was investigated using ten different fluorescein isothiocyanate (FITC) conjugated lectins. In the rete testis epithelium, only PHA-L showed a positive reaction. Efferent ductules in contrary exhibited a wide range of lectin affinity whereas six positive lectins (Con A, LCA, PNA, WGA, PHA-L, PHA-E) were observed. In the connecting and epididymal ducts, four lectins (Con A, WGA, PHA-L, PHA-E) were also detected. GSA-I, UEA-I, and LTA were at all not evident in the epididymal region of the Sudani duck. In conclusion, the correlation between the large areas of the epididymal region occupied by the efferent ductules and the wide range of sugar affinity of this portion may confirm the speculation that efferent ductules might be the primary site of fluid reabsorption in the epididymal region of Sudani duck.

Published
2009
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10. Differentiation of ciliated cells in the terminal bronchioles of neonatal calves.

Author

Marei HE and Abd el-Gawad M

Subjects
Animals, Animals, Newborn, Cattle, Male, Bronchi cytology, Cell Differentiation, Cilia, Epithelial Cells ultrastructure, Respiratory Mucosa cytology
Abstract

The bronchiolar ciliated cells are exquisitely sensitive to injury caused by infection or irritation of the airways. The mechanism by which bronchiolar ciliated cells are renewed following injury or during the normal course of differentiation is still debated. The present study aimed at recognizing the progenitor cell population for bronchiolar ciliated cells during early neonatal life of calves and to demonstrate the course of events occurs during its differentiation into ciliated cells. Scanning electron microscopy of the terminal bronchiolar epithelium revealed two distinct cell types namely ciliated and non-ciliated cells. Transmission electron microscopy revealed ciliated, non-ciliated (Clara), intermediate and basal cells. At least two categories of intermediate cells could be distinguished: intermediate cells with abundant glycogen and variable numbers of organelles; intermediate cells with little glycogen, large numbers of polyribosomes, and variable numbers of basal bodies. We conclude that: (1) both bronchiolar non-ciliated and basal cells serve as progenitors for the bronchiolar ciliated cells; (2) differentiation of ciliated cell from the non-ciliated one involves a transitional cell in which glycogen is lost, polyribosomes are synthesized before the synthesis of basal bodies and cilia.

Published
2001
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