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2. Delta-5 elongase knockout reduces DHA and TAG synthesis coupled with an increase of heat sensitivity in a marine diatom

Author

Zhu, Junkai, primary, Li, Shuangqing, additional, Chen, Weizhong, additional, Xu, Xinde, additional, Wang, Xiaoping, additional, Wang, Xinwei, additional, Han, Jichang, additional, Jouhet, Juliette, additional, Amato, Alberto, additional, Marechal, Eric, additional, Hu, Hanhua, additional, Allen, Andrew E, additional, Gong, Yangmin, additional, and Jiang, Haibo, additional

Published
2024
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9. Nitric Oxide Mediates Nitrite-Sensing and Acclimation and Triggers a Remodeling of Lipids

Author

Dolch, Lina-Juana, Lupette, Josselin, Tourcier, Guillaume, Bedhomme, Mariette, Collin, Séverine, Magneschi, Leonardo, Conte, Melissa, Seddiki, Khawla, Richard, Christelle, Corre, Erwan, Fourage, Laurent, Laeuffer, Frédéric, Richards, Robert, Reith, Michael, Rébeillé, Fabrice, Jouhet, Juliette, McGinn, Patrick, and Maréchal, Eric

Published
2017

10. Investigating mixotrophic metabolism in the model diatom Phaeodactylum tricornutum

Author

Villanova, Valeria, Fortunato, Antonio Emidio, Singh, Dipali, Dal Bo, Davide, Conte, Melissa, Obata, Toshihiro, Jouhet, Juliette, Fernie, Alisdair R., Marechal, Eric, Falciatore, Angela, Pagliardini, Julien, Le Monnier, Adeline, Poolman, Mark, Curien, Gilles, Petroutsos, Dimitris, and Finazzi, Giovanni

Published
2017

13. Adaptive traits of cysts of the snow alga Sanguina nivaloides unveiled by 3D subcellular imaging

Author

Marechal, Eric, primary, Ezzedine, Jade, additional, Uwizeye, Clarisse, additional, Larbi, Gregory Si, additional, Villain, Gaelle, additional, Louwagie, Mathilde, additional, Schilling, Marion, additional, Hagenmuller, Pascal, additional, Gallet, Benoit, additional, Stewart, Adeline, additional, Petroutsos, Dimitris, additional, Devime, Fabienne, additional, Salze, Pascal, additional, Liger, Lucie, additional, Jouhet, Juliette, additional, Dumont, Marie, additional, Ravanel, Stéphane, additional, Amato, Alberto, additional, Valay, Jean-Gabriel, additional, Jouneau, Pierre-Henri, additional, and Falconet, Denis, additional

Published
2023
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26. In silico Discovery of Chemotherapeutic Agents

Author

Birkholtz, Lyn-Marie, Burger, Peter, Aci, Samia, Valadié, Hélène, Costa, Ana Lucia da, Brillet, Loraine, de Beer, Tjaart, Joubert, Fourie, Wells, Gordon, Breton, Vincent, Roy, Sylvaine, Louw, Abraham, Maréchal, Eric, and Sintchenko, Vitali, editor

Published
2010
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29. Role of the Plastid Envelope in the Biogenesis of Chloroplast Lipids

Author

Block, Maryse A., Maréchal, Eric, Joyard, Jacques, Govindjee, editor, Aro, Eva-Mari, editor, Foyer, Christine, editor, Gantt, Elisabeth, editor, Golbeck, John H., editor, Golden, Susan S., editor, Junge, Wolfgang, editor, Michel, Hartmut, editor, Satoh, Kirmiyuki, editor, and Andersson, Bertil, editor

Published
2001
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30. Lipids in Plants and Algae: From Fundamental Science to Industrial Applications

Author

Rebeille, Fabrice, Marechal, Eric, Rebeille, Fabrice, Rebeille, Fabrice, Marechal, Eric, and Rebeille, Fabrice

Abstract

Lipids in Plants and Algae: From Fundamental Science to Industrial Applications, Volume 101 provides in-depth reviews on the most important aspects of the field. Topics in this volume encompass the most recent data about the physical properties of membrane lipids, lipid biosynthesis and metabolism (including glycerolipids, fatty acids, sterols, N-acylethanolamines, prostaglandins, phytoprostane), lipid storage, acyl flux, the dynamic and transport of glycerolipids, and the conversion of fatty acids into hydrocarbons. Lipid metabolism and lipidomics in plants and algae are one of the most challenging areas in biology, not only for fundamental research but also for the sustainable production of valuable molecules for green chemistry, including biofuel and health. Includes sections on fatty acid synthesis, lipid storage and hydrocarbon production Covers biophysics, biochemistry, metabolism and the bioengineering of plant and algae lipids Provides readers with a comprehensive resource on lipid dynamics and fluxes in plants and algae

Published
2022

31. Lipids in Plants and Algae: From Fundamental Science to Industrial Applications Preface

Author

Rebeille, Fabrice, Marechal, Eric, Université Grenoble Alpes (UGA), Physiologie cellulaire et végétale (LPCV), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)

Subjects
Lipide, Algues, Plante, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Abstract

International audience

Published
2022

37. Biofuels from oleaginous algae Principles, industry sectors, challenges

Author

Marechal, Eric, LIPID, Physiologie cellulaire et végétale (LPCV), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Commissariat à l’Energie Atomique et aux Energies Alternatives (programme Fermeture du Cycle du Carbone), Institut Carnot 3BCAR, ANR-11-BTBR-0008,OCEANOMICS,Biotechnologies et bioressources pour la valorisation des écosystèmes marins planctoniques(2011), ANR-10-LABX-0004,CeMEB,Labex(2010), ANR-17-EURE-0003,CBH-EUR-GS,CBH-EUR-GS(2017), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA), and ANR-10-LABX-0004,CeMEB,Mediterranean Center for Environment and Biodiversity(2010)

Subjects
[SDV.EE]Life Sciences [q-bio]/Ecology, environment, [SDV.BIO]Life Sciences [q-bio]/Biotechnology
Abstract

International audience; (~500-800 signes) : Résumé du texte-Résumé du texte Certains organismes photosynthétiques sont capables de capturer le CO2 atmosphérique et de produire une biomasse riche en huile. Cette huile est considérée de ce fait comme une ressource renouvelable, qui pourrait devenir une alternative aux hydrocarbures fossiles. Cet article fournit une définition détaillée de ce qu'on entend par microalgue, huile, biocarburant, et donne un état de l'art des technologies de culture, de récolte, d'extraction d'huile et de conversion en biodiesel, du laboratoire à l'échelle pilote, soulignant les verrous biotechnologiques et technologiques à lever dans l'avenir.

Published
2021

39. Plastidial ACP Δ9‐desaturase modulates EPA biosynthesis and TAG accumulation in Phaeodactylum tricornutum

Author

Richard, Smith, Jouhet, Juliette, Gandini, Chiara, Nekrasova, Vladimir, Marechal, Eric, Napier, Johnathan A., Sayanova, Olga, Rothamsted Research, LIPID, Physiologie cellulaire et végétale (LPCV), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS), Biotechnology and Biological Sciences Research Council (BBSRC), UK (Grant BB/L002957/1), Biotechnology and Biological Sciences Research Council (BBSRC), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)

Subjects
lipids, eicosapentaenoic acid, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, omega-3 PUFA biosynthesis, lipids (amino acids, peptides, and proteins), [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics, Phaeodactylum tricornutum, Δ9-desaturase, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
Abstract

International audience; The unicellular marine diatom Phaeodactylum tricornutum accumulates up to 35% eicosapentaenoic acid (EPA, 20:5n3) and has been used as a model organism to study long chain polyunsaturated fatty acids (LC-PUFA) biosynthesis due to an excellent annotated genome sequence and established transformation system. In P. tricornutum, the majority of EPA accumulates in polar lipids, especially in galactolipids such as mono- and di-galactosyldiacylglycerol (MGDG and DGDG). LC-PUFA biosynthesis is considered to start from oleic acid (18:1n9). EPA can be synthesized via a series of desaturation and elongation steps occurring at the endoplasmic reticulum and newly synthesized EPA is then imported into the plastids for incorporation into galactolipids via an unknown route. The basis for the flux of EPA is fundamental to understanding LC-PUFA biosynthesis in diatoms. We used P. tricornutum to study acyl modifying activities, upstream of 18:1n9, on subsequent LC-PUFA biosynthesis. We identified the gene coding for the plastidial acyl carrier protein Δ9-desaturase, a key enzyme in fatty acid modification and analysed the impact of overexpression and knock out of this gene on glycerolipid metabolism. This revealed a previously unknown role of this soluble desaturase in EPA synthesis and production of TAG. This study provides further insight into the distinctive nature of lipid metabolism in the marine diatom P. tricornutum and suggests additional approaches for tailoring oil composition in microalgae.

Published
2021

41. Phosphate deprivation induces transfer of DGDG galactolipid from chloroplast to mitochondria

Author

Jouhet, Juliette, Marechal, Eric, Baldan, Barbara, Bligny, Richard, Joyard, Jacques, and Block, Maryse A.

Subjects
Plants -- Research, Biological sciences
Abstract

In many soils plants have to grow in a shortage of phosphate, leading to development of phosphate-saving mechanisms. At the cellular level, these mechanisms include conversion of phospholipids into glycolipids, mainly digalactosyldiacylglycerol (DGDG). The lipid changes are not restricted to plastid membranes where DGDG is synthesized and resides under normal conditions. In plant cells deprived of phosphate, mitochondria contain a high concentration of DGDG, whereas mitochondria have no glycolipids in control cells. Mitochondria do not synthesize this pool of DGDG, which structure is shown to be characteristic of a DGD type enzyme present in plastid envelope. The transfer of DGDG between plastid and mitochondria is investigated and detected between mitochondria-closely associated envelope vesicles and mitochondria. This transfer does not apparently involve the endomembrane system and would rather be dependent upon contacts between plastids and mitochondria. Contacts sites are favored at early stages of phosphate deprivation when DGDG cell content is just starting to respond to phosphate deprivation.

Published
2004

43. Biosynthesis of Long Chain Alkyl Diols and Long Chain Alkenols in Nannochloropsis spp. (Eustigmatophyceae)

Author

Balzano, Sergio, Villanueva, Laura, de Bar, Marijke, Sahonero Canavesi, Diana X., Yildiz, Caglar, Engelmann, Julia C., Marechal, Eric, Lupette, Josselin, Sinninghe Damsté, Jaap S., Schouten, Stefan, Organic geochemistry & molecular biogeology, Organic geochemistry, Royal Netherlands Institute for Sea Research (NIOZ), Faculty of Geosciences, Utrecht University, Faculty of Geosciences, LIPID, Physiologie cellulaire et végétale (LPCV), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), European Project: 339206,EC:FP7:ERC,ERC-2013-ADG,DIOLS(2014), Utrecht University [Utrecht], Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), European Research Council (ERC) 339206Netherlands Earth System Science Centre (NESSC), ANR-11-BTBR-0008,OCEANOMICS,Biotechnologies et bioressources pour la valorisation des écosystèmes marins planctoniques(2011), ANR-17-EURE-0003,CBH-EUR-GS,CBH-EUR-GS(2017), Organic geochemistry & molecular biogeology, Organic geochemistry, Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG)

Subjects
0106 biological sciences, Lipid biosynthesis, Physiology, polyketide synthase, Plant Science, 01 natural sciences, Substrate Specificity, Hydroxylation, Fatty Acids, Monounsaturated, chemistry.chemical_compound, Diols, Microalgae, Nannochloropsis, Enoyl-CoA Hydratase, chemistry.chemical_classification, 0303 health sciences, biology, Hydroxylated fatty acids, General Medicine, [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics, alkenols, Alkenols, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], Enzymes, Biochemistry, hydroxylated fatty acids, Metabolic pathway, Fatty Acid Elongases, diols, Alkenes, 03 medical and health sciences, Polyketide, Biosynthesis, Acetyltransferases, Polyketide synthase, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], 030304 developmental biology, Regular Papers, Fatty acid, Cell Biology, transcriptomic, bioproduct, biology.organism_classification, chemistry, Alcohols, biology.protein, gene expression, Bioproduct, Polyketide Synthases, 010606 plant biology & botany
Abstract

We investigated potential biosynthetic pathways of long chain alkenols (LCAs), long chain alkyl diols (LCDs), and long chain hydroxy fatty acids (LCHFAs) in Nannochloropsis oceanica and Nannochloropsis gaditana, by combining culturing experiments with genomic and transcriptomic analyses. Incubation of Nannochloropsis spp. in the dark for 1 week led to significant increases in the cellular concentrations of LCAs and LCDs in both species. Consistently, 13C-labelled substrate experiments confirmed that both LCA and LCD were actively produced in the dark from C14–18 fatty acids by either condensation or elongation/hydroxylation, although no enzymatic evidence was found for the former pathway. Nannochloropsis spp. did, however, contain (i) multiple polyketide synthases (PKSs) including one type (PKS-Clade II) that might catalyze incomplete fatty acid elongations leading to the formation of 3-OH-fatty acids, (ii) 3-hydroxyacyl dehydratases (HADs), which can possibly form Δ2/Δ3 monounsaturated fatty acids, and (iii) fatty acid elongases (FAEs) that could elongate 3-OH-fatty acids and Δ2/Δ3 monounsaturated fatty acids to longer products. The enzymes responsible for reduction of the long chain fatty acids to LCDs and LCAs are, however, unclear. A putative wax ester synthase/acyl coenzyme A (acyl-CoA): diacylglycerol acyltransferase is likely to be involved in the esterification of LCAs and LCDs in the cell wall. Our data thus provide useful insights in predicting the biosynthetic pathways of LCAs and LCDs in phytoplankton suggesting a key role of FAE and PKS enzymes.

Published
2019

44. Abscisic acid signaling through cyclic ADP-ribose in plants

Author

Wu, Yan, Kuzma, Jennifer, Marechal, Eric, Graeff, Richard, Lee, Hon Cheung, Foster, Randy, and Chua, Nam-Hai

Subjects
Plant hormones -- Research -- Physiological aspects, Abscisic acid -- Research -- Physiological aspects, Science and technology, Physiological aspects, Research
Abstract

Abscisic acid (ABA) is the primary hormone that mediates plant responses to stresses such as cold, drought, and salinity. Single-cell microinjection experiments in tomato were used to identify possible intermediates involved in ABA signal transduction. Cyclic ADP-ribose (cADPR) was identified as a signaling molecule in the ABA response and was shown to exert its effects by way of calcium. Bioassay experiments showed that the amounts of cADPR in Arabidopsis thaliana plants increased in response to ABA treatment and before ABA-induced gene expression., Plants endure environmental challenges such as drought, salinity, or cold by adjusting rather than escaping. These responses are mediated by ABA (1), which through unknown signals affects the regulation of [...]

Published
1997

45. Biosynthesis of Long Chain Alkyl Diols and Long Chain Alkenols in Nannochloropsis spp. (Eustigmatophyceae)

Author

Organic geochemistry & molecular biogeology, Organic geochemistry, Balzano, Sergio, Villanueva, Laura, de Bar, Marijke, Sahonero Canavesi, Diana X., Yildiz, Caglar, Engelmann, Julia C., Marechal, Eric, Lupette, Josselin, Sinninghe Damsté, Jaap S., Schouten, Stefan, Organic geochemistry & molecular biogeology, Organic geochemistry, Balzano, Sergio, Villanueva, Laura, de Bar, Marijke, Sahonero Canavesi, Diana X., Yildiz, Caglar, Engelmann, Julia C., Marechal, Eric, Lupette, Josselin, Sinninghe Damsté, Jaap S., and Schouten, Stefan

Published
2019

47. Plastids: Methods and Protocol. Series Method in Molecular Biology, Vol.1829

Author

Marechal, Eric, Physiologie cellulaire et végétale (LPCV), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Martin-Laffon, Jacqueline, and Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG)

Subjects
[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

49. Nuclear genome sequence of the plastid-lacking cryptomonad Goniomonas avonlea provides insights into the evolution of secondary plastids

Author

Cenci, Ugo, Sibbald, Shannon J., Curtis, Bruce A., Kamikawa, Ryoma, Eme, Laura, Moog, Daniel, Henrissat, Bernard, Marechal, Eric, Chabi, Malika, Djemiel, Christophe, Roger, Andrew J., Kim, Eunsoo, Archibald, John M., Cenci, Ugo, Sibbald, Shannon J., Curtis, Bruce A., Kamikawa, Ryoma, Eme, Laura, Moog, Daniel, Henrissat, Bernard, Marechal, Eric, Chabi, Malika, Djemiel, Christophe, Roger, Andrew J., Kim, Eunsoo, and Archibald, John M.

Abstract

Background: The evolution of photosynthesis has been a major driver in eukaryotic diversification. Eukaryotes have acquired plastids (chloroplasts) either directly via the engulfment and integration of a photosynthetic cyanobacterium (primary endosymbiosis) or indirectly by engulfing a photosynthetic eukaryote (secondary or tertiary endosymbiosis). The timing and frequency of secondary endosymbiosis during eukaryotic evolution is currently unclear but may be resolved in part by studying cryptomonads, a group of single-celled eukaryotes comprised of both photosynthetic and non-photosynthetic species. While cryptomonads such as Guillardia theta harbor a red algal-derived plastid of secondary endosymbiotic origin, members of the sister group Goniomonadea lack plastids. Here, we present the genome of Goniomonas avonlea-the first for any goniomonad-to address whether Goniomonadea are ancestrally non-photosynthetic or whether they lost a plastid secondarily. Results: We sequenced the nuclear and mitochondrial genomes of Goniomonas avonlea and carried out a comparative analysis of Go. avonlea, Gu. theta, and other cryptomonads. The Go. avonlea genome assembly is similar to 92 Mbp in size, with 33,470 predicted protein-coding genes. Interestingly, some metabolic pathways (e.g., fatty acid biosynthesis) predicted to occur in the plastid and periplastidal compartment of Gu. theta appear to operate in the cytoplasm of Go. avonlea, suggesting that metabolic redundancies were generated during the course of secondary plastid integration. Other cytosolic pathways found in Go. avonlea are not found in Gu. theta, suggesting secondary loss in Gu. theta and other plastid-bearing cryptomonads. Phylogenetic analyses revealed no evidence for algal endosymbiont-derived genes in the Go. avonlea genome. Phylogenomic analyses point to a specific relationship between Cryptista (to which cryptomonads belong) and Archaeplastida. Conclusion: We found no convincing genomic or phylogenomic evidenc

Published
2018
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50. Cinquième chapitre: De la curiosité à l’application (Collectif incluant Eric Maréchal)

Author

Marechal, Eric, Physiologie cellulaire et végétale (LPCV), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Catherine Jessus, Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), and Martin-Laffon, Jacqueline

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], Ouvrage collectif, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017

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