46 results on '"Marchioro L"'
Search Results
2. Comparability study of different analytes, from Wantai Wan200+ instrument using routine laboratory assays
- Author
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Talli, I., primary, Padoan, A., additional, Furlan, G., additional, Cosma, C., additional, Marchioro, L., additional, Zaninotto, M., additional, Basso, D., additional, and Plebani, M., additional
- Published
- 2024
- Full Text
- View/download PDF
3. Evaluation of diagnostic reliability of DCA 2000 for rapid and simple monitoring of HbA1c
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Arsie, M.P., Marchioro, L., Lapolla, A., Giacchetto, G.F., Bordin, M.R., Rizzotti, P., and Fedele, D.
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- 2000
- Full Text
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4. KETAMINE: URINARY DETECTION BY IMMUNOMETRIC TEST ON-SITE. COMPARISON BETWEEN TWO DIFFERENT POINT OF CARE METHODS WITH CONFIRMATION ANALYSIS BY HPLC/MS
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Marchioro, L., Pavan, I., Bologna, L., Artusi, C., Ivanova, M., Liverani, A., Polo, G., Zaninotto, M., and Plebani, M.
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- 2010
5. Radicalar probes to measure the action of energy on granular materials
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Baron, M., Chamayou, A., Marchioro, L, and Raffi, J
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- 2005
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6. Effect of a low glycaemic index diet during pregnancy on maternal and cord blood metabolomic profiles: results from the ROLO randomized controlled trial
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Marchioro, L, Geraghty, AA, Uhl, O, Shokry, E, O'Brien, EC, Koletzko, B, McAuliffe, FM, Marchioro, L, Geraghty, AA, Uhl, O, Shokry, E, O'Brien, EC, Koletzko, B, and McAuliffe, FM
- Abstract
BACKGROUND: Elevated post-prandial blood glucose during pregnancy has been associated with adverse pregnancy and offspring outcomes, such as maternal gestational diabetes and excessive foetal growth. The ROLO Study is a randomized controlled trial (RCT) investigating the effect of a low glycaemic index (GI) diet in pregnancy to prevent foetal macrosomia (birth weight > 4000 g). We described the impact of a low-GI diet on the maternal and feto-placental unit metabolism by studying how the ROLO intervention affected maternal and cord blood metabolomes. METHODS: Fasting maternal plasma samples pre- and post-intervention of 51 pregnant women and 132 cord blood samples were measured with a targeted metabolomics approach using liquid-chromatography coupled to tandem mass spectrometry. The differences between RCT groups were explored via multivariate models with covariates correction. Significance was set at Bonferroni-corrected level of 0.05. RESULTS: A total of 262 metabolites species, sums and ratios were investigated. While no metabolite reached statistical significance after Bonferroni correction, many maternal phospholipids and acylcarnitines were elevated in the intervention group at uncorrected 0.05 alpha level. Most species contained saturated and monounsaturated fatty acid chains with 16 or 18 carbon atoms. In cord blood, no differences were identified between RCT groups. CONCLUSIONS: A low-GI diet in pregnancy was associated with a trend to modest but consistent changes in maternal lipid and fatty acid metabolism. The intervention seemed not to affect foetal metabolism. Our exploratory findings may be used to direct further investigations about low GI diets before and during pregnancy, to improve patient care for pre-conceptional and pregnant women with lipid dysregulations and potentially modulate the offspring's risk for future metabolic diseases. TRIAL REGISTRATION: Current Controlled Trials ISRCTN54392969.
- Published
- 2019
7. Caesarean section, but not induction of labour, is associated with major changes in cord blood metabolome
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Marchioro, L, Shokry, E, Geraghty, AA, O'Brien, EC, Uhl, O, Koletzko, B, McAuliffe, FM, Marchioro, L, Shokry, E, Geraghty, AA, O'Brien, EC, Uhl, O, Koletzko, B, and McAuliffe, FM
- Abstract
The physiology of how prelabour caesarean section (PCS) and induction of labour (IOL) in comparison to spontaneous vaginal delivery (SVD) has not been fully clarified yet. We measured 201 cord blood (CB) phospholipids and energy metabolites via LC/MS-MS in 109 newborns from the ROLO Kids study; metabolites were compared across the three parturition groups via linear mixed models with correction for multiple testing. In comparison to SVD, PCS babies had lower non-esterified fatty acids (NEFA), including sum of NEFA (p < 0.001), and trends for lower acylcarnitines. The lack of hormonal stimuli, especially catecholamines and cortisol, may underlie the metabolic changes involving gluconeogenesis from fatty acid oxidation (FAO) in PCS born infants. IOL and SVD infants showed no significant differences in metabolites, but ratios estimating carnitine palmitoyltrasferase 1 activity (precursor for FAO) were slightly higher in IOL than in SVD. Thus, IOL does not induce metabolic disadvantage when compared to SVD, though post-natal gluconeogenesis might start earlier due to the artificial solicitation in IOL. These data shed light on the physiology of parturition and may contribute to understand how mode of delivery might modulate future metabolic risks.
- Published
- 2019
8. Maternal BMI and gestational diabetes mellitus: Impacts on the maternal and cord blood metabolome
- Author
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Shokry, E.S., primary, Marchioro, L., additional, Uhl, O., additional, Bermúdez, M.G., additional, García-Santos, J.A., additional, Segura, M.T., additional, Campoy, C., additional, and Koletzko, B., additional
- Published
- 2018
- Full Text
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9. Assessment of alcohol consumption in liver transplant candidates and recipients: the best combination of the tools available
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Piano, S, Marchioro, L, Gola, E, Rosi, S, Morando, F, Cavallin, M, Sticca, A, Fasolato, S, Forza, G, Chiara Frigo, A, Plebani, Mario, Zanus, G, Cillo, U, Gatta, A, and Angeli, Paolo
- Subjects
Male ,Alcohol Drinking ,Ethanol ,Transferrin ,Glucuronates ,Middle Aged ,Liver Transplantation ,End Stage Liver Disease ,Alcoholism ,ROC Curve ,Recurrence ,Odds Ratio ,Humans ,Female ,Prospective Studies ,Biomarkers ,Aged - Abstract
The detection of alcohol consumption in liver transplant candidates (LTCs) and liver transplant recipients (LTRs) is required to enable a proper assessment of transplant eligibility and early management of alcohol relapse, respectively. In this clinical setting, urinary ethyl glucuronide (uEtG), the Alcohol Use Disorders Identification Test for Alcohol Consumption (AUDIT-c), serum ethanol, urinary ethanol, carbohydrate-deficient transferrin (CDT), and other indirect markers of alcohol consumption were evaluated and compared prospectively in 121 LTCs and LTRs. Alcohol consumption was diagnosed when AUDIT-c results were positive or it was confirmed by a patient's history in response to abnormal results. Alcohol consumption was found in 30.6% of the patients. uEtG was found to be the strongest marker of alcohol consumption (odds ratio = 414.5, P 0.001) and provided a more accurate prediction rate of alcohol consumption [area under receiving operating characteristic (ROC) curve = 0.94] than CDT (area under ROC curve = 0.63, P 0.001) and AUDIT-c (area under ROC curve = 0.73, P 0.001). The combination of uEtG and AUDIT-c showed higher accuracy in detecting alcohol consumption in comparison with the combination of CDT and AUDIT-c (area under ROC curve = 0.98 versus 0.80, P 0.001). Furthermore, uEtG was the most useful marker for detecting alcohol consumption in patients with negative AUDIT-c results. In conclusion, the combination of AUDIT-c and uEtG improves the detection of alcohol consumption in LTCs and LTRs. Therefore, they should be used routinely for these patients.
- Published
- 2013
10. Determinazione di Etil-Glucuronide in gruppi selezionati di soggetti: proposta di cut-off in ragione dei risultati ottenuti
- Author
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Marchioro, L., Trombin, A., Toffolon, S., Angeli, Paolo, Miolo, Giorgia, and Plebani, Mario
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Ethylglucuronide ,DRI-EtG EIA ,Ethylglucuronide, DRI-EtG EIA - Published
- 2009
11. Quantitative macronutrient intakes in Thai infants exclusively fed human milk at 2 and 4 months of age.
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Vyllioti, A. T., Patro-Golab, B., Demmelmair, H., Horak, J., Marchioro, L., Shokry, E., Grote, V., Jakubowicz, C., Flemmer, A. W., Koletzko, B., and Dumrongwongsiri, O.
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- 2022
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12. Quantification de processus radiculaires induits dans diverses poudres
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Piccerelle, P., Jacques Raffi, Prinderre, P., alain chamayou, Marchioro, L., Michel Baron, Poudres et procédés - Ecole des Mines Albi-Carmaux, IMT École nationale supérieure des Mines d'Albi-Carmaux (IMT Mines Albi), and Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)
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[SPI]Engineering Sciences [physics] ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
- Published
- 2003
13. Ruolo degli anticorpi anti-ox-LDL nella malattia diabetica
- Author
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Rossetti, C., Piarulli, F., Lapolla, Annunziata, Marolla, M. E., Sartore, G., Marchioro, L., Fiore, C., and Fedele, D.
- Published
- 2002
14. Evaluation of diagnostic reability of DCA 2000 for rapid and simple monitoring of HbA1c
- Author
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Arsie, M. P., Marchioro, L., Lapolla, Annunziata, Giachetto, G. F., Bordin, M. R., Rizzotti, P., and Fedele, Domenico
- Published
- 2000
15. 1368 URINARY ETHYL GLUCURONIDE IMPROVES THE DETECTION OF ALCOHOL CONSUMPTION IN LIVER TRANSPLANT CANDIDATES AND RECIPIENTS
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Piano, S., primary, Cardillo, S., additional, Marchioro, L., additional, Morando, F., additional, Rosi, S., additional, Romano, A., additional, Cavallin, M., additional, Plebani, M., additional, Gatta, A., additional, and Angeli, P., additional
- Published
- 2012
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16. OC-23 Urinary ethyl glucuronide improves the detection of alcohol consumption in liver transplant candidates and recipients
- Author
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Piano, S., primary, Cardillo, S., additional, Marchioro, L., additional, Morando, F., additional, Rosi, S., additional, Romano, A., additional, Cavallin, M., additional, Plebani, M., additional, Gatta, A., additional, and Angeli, P., additional
- Published
- 2012
- Full Text
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17. Pancreolauryl test in chronic pancreatitis
- Author
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Panucci, A., Angonese, C., Del Favero, G., Fabris, C., Marchioro, L., Basso, D., Di Mario, F., and Naccarato, R.
- Published
- 1986
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18. Enlargement of perihepatic lymph nodes in relation to biochemical data and liver histology in patients with chronic hepatitis C.Preliminary results
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Carlotto, A., primary, Marchioro, L., additional, Ferretto, R., additional, Stevan, D., additional, Visona, A., additional, and Marranconi, F., additional
- Published
- 2002
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19. Tissue polypeptide antigen (TPA) in pancreatic cancer diagnosis.
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Panucci, A, Fabris, C, Del Favero, G, Basso, D, Marchioro, L, Piccoli, A, Burlina, A, and Naccarato, R
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- 1985
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20. Is tissue polypeptide antigen more accurate than serum CEA for diagnosing pancreatic cancer?
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Panucci, A, Fabris, C, Del Favero, G, Basso, D, Di Mario, F, Marchioro, L, Piccoli, A, Lise, M, Burlina, A, and Naccarato, R
- Abstract
Tissue polypeptide antigen (TPA) and carcinoembryonic antigen (CEA) were determined in the sera of 36 control subjects, 30 patients with pancreatic cancer, 35 with chronic pancreatitis and 25 with non-pancreatic digestive disease to evaluate their role in detecting pancreatic malignancy. Abnormal values of TPA and CEA were found in 28 and 19 of 30 patients with pancreatic cancer, and in four and seven of 35 patients with chronic pancreatitis, respectively. Raised titres of TPA were observed more often than equivalent serum CEA in simulated pancreatic diseases. The receiver-operating (ROC) characteristic curves showed that TPA was more discriminating than CEA in detecting pancreatic cancer. Specificity was enhanced when both titres were abnormally high and sensitivity when one titre was raised, but the diagnostic accuracy of TPA alone has not improved, which satisfactorily discriminates pancreatic cancer from chronic pancreatitis. [ABSTRACT FROM PUBLISHER]
- Published
- 1986
21. Slow-like electrostimulation switches on slow myosin in denervated fast muscle
- Author
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Carraro, U., primary, Catani, C., additional, Belluco, S., additional, Cantini, M., additional, and Marchioro, L., additional
- Published
- 1986
- Full Text
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22. The ULK1 effector BAG2 regulates autophagy initiation by modulating AMBRA1 localization.
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Sankar DS, Kaeser-Pebernard S, Vionnet C, Favre S, de Oliveira Marchioro L, Pillet B, Zhou J, Stumpe M, Kovacs WJ, Kressler D, Antonioli M, Fimia GM, and Dengjel J
- Subjects
- Humans, HEK293 Cells, Phosphorylation, Autophagy-Related Proteins metabolism, Protein Binding, Class III Phosphatidylinositol 3-Kinases metabolism, HeLa Cells, Vesicular Transport Proteins metabolism, Vesicular Transport Proteins genetics, Molecular Chaperones, Autophagy, Autophagy-Related Protein-1 Homolog metabolism, Adaptor Proteins, Signal Transducing metabolism, Intracellular Signaling Peptides and Proteins metabolism
- Abstract
Autophagy initiation is regulated by the ULK1 kinase complex. To gain insights into functions of the holo-complex, we generated a deep interactome by combining affinity purification- and proximity labeling-mass spectrometry of all four complex members: ULK1, ATG13, ATG101, and RB1CC1/FIP200. Under starvation conditions, the ULK1 complex interacts with several protein and lipid kinases and phosphatases, implying the formation of a signalosome. Interestingly, several selective autophagy receptors also interact with ULK1, indicating the activation of selective autophagy pathways by nutrient starvation. One effector of the ULK1 complex is the HSC/HSP70 co-chaperone BAG2, which regulates the subcellular localization of the VPS34 lipid kinase complex member AMBRA1. Depending on the nutritional status, BAG2 has opposing roles. In growth conditions, the unphosphorylated form of BAG2 sequesters AMBRA1, attenuating autophagy induction. In starvation conditions, ULK1 phosphorylates BAG2 on Ser31, which supports the recruitment of AMBRA1 to the ER membrane, positively affecting autophagy., Competing Interests: Declaration of interests The authors declare no competing interests., (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
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- 2024
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23. Evaluation of the Comparability of Wantai Wan200+ Instrument with Routine Laboratory Assays for 21 Different Analytes.
- Author
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Talli I, Padoan A, Cosma C, Furlan G, Zaninotto M, Marchioro L, Galozzi P, Basso D, and Plebani M
- Abstract
Background : We compared the performance of 21 different assays performed by the Wantai Wan200+ (Wantai BioPharm, Beijing, China) with respect to other methods in use at the University Hospital of Padova (AOPD), Italy. Methods : The plasma (P) or serum (S) of 5027 leftover samples, collected from May to Sept 2023, was either analyzed or frozen at -20 °C. Beckman DXI800 (DXI), Roche Cobas 8000 e801 (RC), Snibe Maglumi 4000 plus (SM), DiaSorin Liaison XL (DL) and Binding Site Optilite (BS) equipment were used at the AOPD. P-procalcitonin (PCT), DXI; P-Troponin I (TnI), DXI; S-CA125, DXI; S-free PSA (f-PSA), DXI; S-total PSA (t-PSA), DXI; S-IL6, SM; P-Troponin T (TnT), RC; P-NT-proBNP, RC; P-Neuron-Specific Enolase (NSE), RC; S-CA15-3, DL; S-CA19-9, DL; S-AFP, DL; and S-CEA, DL were tested in fresh samples. P-Myoglobin (Myo), DXI; P-Cyfra21-1, RC; S-β2 microglobulin (B2MIC), BS; S-HE4, SM; S-PGI, SM; S-PGII, SM; S-CA72-4, SM; and S-CA50, SM were analyzed in frozen and thawed samples. Bland-Altman (BA), Passing-Bablok (PB) and Cohen's Kappa (CKa) metrics were used as statistics. Results : An excellent comparability profile was found for 11 analytes. For example, the t-PSA CKa was 0.94 (95%CI: 0.90 to 0.98), and the PB slope and intercept were 1.02 (95%CI: 0.99 to 1.03) and 0.02 (95%CI: 0.01 to 0.03), respectively; the BA bias was 2.25 (95%CI: -0.43 to 4.93). Ten tested measurands demonstrated a suboptimal comparability profile. Biological variation in EFLM (EuBIVAS) performance specifications was evaluated to assess the clinical relevance of measured biases. Conclusions : Evaluation of the Wantai Wan200+'s performance suggests that between-method differences did not exceed the calculated bias. Metrological traceability may influence the comparisons obtained for some measurands.
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- 2024
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24. Analytical and clinical evaluations of SNIBE Maglumi chemiluminescent immunoassay for the detection of SARS-CoV-2 antigen in salivary samples.
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Padoan A, Talli I, Cosma C, Moz S, Furlan G, Navaglia F, Marchioro L, Zaninotto M, Basso D, and Plebani M
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- Humans, SARS-CoV-2, Immunologic Tests, Antigens, Viral, Biological Assay, Sensitivity and Specificity, COVID-19 diagnosis
- Abstract
Objectives: In this study, we describe the analytical and clinical performances of the SNIBE Maglumi SARS-CoV-2 antigen fully-automated chemiluminescent immunoassay (MAG-CLIA) on salivary samples., Methods: Limit of detection (LOD), linearity and precision were tested for values close to or below the declared LOD. Clinical performance of MAG-CLIA was evaluated on leftover salivary samples from the healthcare workers (HCW) surveillance program, at the University-Hospital of Padova. Salivary samples were analyzed by Lumipulse G SARS-CoV-2 Ag, and in case where the values exceeded 0.41 ng/L, further testing was conducted using TaqPathTM COVID-19 RT-PCR (Applied Biosystems, Thermo Fisher Scientific)., Results: The estimated MAG-CLIA LOD was 3 ng/L, with repeatability of 7.5 %. Good linearity was demonstrated by diluting two samples at 52.7 ng/L and 211.4 ng/L. Of the 228 HCW samples, 59/228 (25.9 %) were positive, 169/228 (74.1 %) were negative. MAG-CLIA SARS-CoV-2 sAg median level (and interquartile range [IQR]) was 5.03 ng/L (<0.001-35.8 ng/L) for positive and <0.001 ng/L (<0.001 ng/L) for negative samples. MAG-CLIA AUC was 0.795 (95 % CI: 0.720-0.871). Using the best cut-off, 3.5 ng/L, sensitivity and specificity were 57.1 % (95 % CI: 42.2-71.2 %) and 97.0 % (95 % CI: 93.2-99.0 %), respectively. The agreement with the molecular assay was 88.1 % (Cohen's kappa 0.606 [SE=0.066, p<0.001])., Conclusions: The analytical performances of MAG-CLIA are satisfactory, also when values below LOD were tested. In saliva samples, although specificity was elevated, clinical performance was not comparable with that on nasopharyngeal swabs (NPS)., (© 2023 the author(s), published by De Gruyter, Berlin/Boston.)
- Published
- 2023
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25. SARS-CoV-2 specific T-cell humoral response assessment after COVID-19 vaccination using a rapid direct real-time PCR amplification.
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Cosma C, Galla L, Padoan A, Furlan G, Marchioro L, Zaninotto M, Basso D, and Plebani M
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- Humans, COVID-19 Vaccines, Real-Time Polymerase Chain Reaction, SARS-CoV-2, Vaccination, Lithium, Immunoglobulin G, Antibodies, Viral, COVID-19 Testing, T-Lymphocytes, COVID-19 diagnosis, COVID-19 prevention & control
- Abstract
Objectives: The SARS-CoV-2 immune response is mediated by both humoral and cellular immunity. In this study, SARS-CoV-2 specific cellular immunity was tested by a novel direct real-time PCR (dRT-PCR) assay, targeting mRNA of CXCL10 , and compared with respect to an ELISA measuring interferon gamma (IFN-γ) release., Methods: Whole blood (Li-He) and serum samples were collected from 92 healthcare workers (HCW), with three doses of homologous (Pfizer/BioNTech, n=74) or heterologous (Pfizer/BioNTech and Vaxzevria or Moderna, n=18) vaccinations. Li-He samples were incubated with SCV2 PANEL-1-T-ACTIVATION (Hyris srl, Lodi, Italy), or CoV-2 IGRA TUBE ELISA (Euroimmune, Lubeck, Germany). CXCL10 mRNA expression was analyzed by bCube/bApp (Hyris), while IFN-γ was evaluated by quant-T-Cell SARS-CoV-2 ELISA (Euroimmune). Anti-SARS-CoV-2 S-RBD IgG levels were measured in sera using a CLIA assay (Snibe, Shenzen, China)., Results: Imprecision of dRT-PCR assay was found to be satisfactory, and the two methods for measuring T cell immunity to SARS-CoV-2 peptides agreed in 82/87 (94.2%) of results. At qualitative dRT-PCR analyses, 81 subjects (93.2%) resulted as reactive to SARS-CoV-2 peptides, 3 (3.4%) were borderline and 3 were negative (3.4%). At univariate and multivariate analyses of quantitative dRT-PCR mRNA of CXCL10 and IFN-γ release results showed no difference between HCW with previous infection, homologous/heterologous vaccination, or demographical features. Anti-SARS-CoV-2 S-RBD IgG was associated with the previous infection and the time between the last vaccination or positivity., Conclusions: Direct RT-PCR appeared accurate for determining the presence or absence of immunoreactivity of SARS-CoV-2 specific T cells, especially when rapid analyses are required, such as for organ transplantation., (© 2023 Walter de Gruyter GmbH, Berlin/Boston.)
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- 2023
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26. Tube shaking and pneumatic transportation: impact on presepsin concentrations measured by both fully automated and POCT analyzers.
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Cosma C, Marchioro L, Zaninotto M, Mion MM, and Plebani M
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- Humans, Lipopolysaccharide Receptors, Biomarkers, Blood Specimen Collection, Peptide Fragments
- Published
- 2022
- Full Text
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27. Maternal Body Mass Index, Early-Pregnancy Metabolite Profile, and Birthweight.
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Wahab RJ, Jaddoe VWV, Voerman E, Ruijter GJG, Felix JF, Marchioro L, Uhl O, Shokry E, Koletzko B, and Gaillard R
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- Adult, Amino Acids blood, Amino Acids metabolism, Carnitine blood, Carnitine metabolism, Fatty Acids, Nonesterified blood, Fatty Acids, Nonesterified metabolism, Female, Humans, Maternal Age, Metabolomics, Obesity, Maternal blood, Obesity, Maternal diagnosis, Phospholipids blood, Phospholipids metabolism, Pregnancy, Pregnancy Trimester, Second blood, Pregnancy Trimester, Second metabolism, Pregnancy Trimester, Third blood, Pregnancy Trimester, Third metabolism, Prospective Studies, Risk Factors, Birth Weight, Body Mass Index, Obesity, Maternal metabolism
- Abstract
Context: Maternal prepregnancy body mass index (BMI) has a strong influence on gestational metabolism, but detailed metabolic alterations are unknown., Objective: First, to examine the associations of maternal prepregnancy BMI with maternal early-pregnancy metabolite alterations. Second, to identify an early-pregnancy metabolite profile associated with birthweight in women with a higher prepregnancy BMI that improved prediction of birthweight compared to glucose and lipid concentrations., Design, Setting, and Participants: Prepregnancy BMI was obtained in a subgroup of 682 Dutch pregnant women from the Generation R prospective cohort study., Main Outcome Measures: Maternal nonfasting targeted amino acids, nonesterified fatty acid, phospholipid, and carnitine concentrations measured in blood serum at mean gestational age of 12.8 weeks. Birthweight was obtained from medical records., Results: A higher prepregnancy BMI was associated with 72 altered amino acids, nonesterified fatty acid, phospholipid and carnitine concentrations, and 6 metabolite ratios reflecting Krebs cycle, inflammatory, oxidative stress, and lipid metabolic processes (P-values < 0.05). Using penalized regression models, a metabolite profile was selected including 15 metabolites and 4 metabolite ratios based on its association with birthweight in addition to prepregnancy BMI. The adjusted R2 of birthweight was 6.1% for prepregnancy BMI alone, 6.2% after addition of glucose and lipid concentrations, and 12.9% after addition of the metabolite profile., Conclusions: A higher maternal prepregnancy BMI was associated with altered maternal early-pregnancy amino acids, nonesterified fatty acids, phospholipids, and carnitines. Using these metabolites, we identified a maternal metabolite profile that improved prediction of birthweight in women with a higher prepregnancy BMI compared to glucose and lipid concentrations., (© The Author(s) 2021. Published by Oxford University Press on behalf of the Endocrine Society.)
- Published
- 2022
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28. Endothelial dysfunction and Mid-Regional proAdrenomedullin: What role in SARS-CoV-2 infected Patients?
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Zaninotto M, Mion MM, Marchioro L, Padoan A, and Plebani M
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- Biomarkers, Endothelium virology, Humans, Prognosis, SARS-CoV-2, Adrenomedullin blood, COVID-19 diagnosis, Endothelium physiopathology, Protein Precursors blood
- Abstract
Background: Endothelial dysfunction, a major complication of SARS-CoV-2 infectionplaying a key-role in multi-organ damage, carries high risk of mortality., Aim: To investigate the potential role of Mid-Regional pro-Adrenomedullin (MR-proADM) in detecting endothelial damage with a view to stratifying the risk of adverse events (length of stay, death, admission in Intensive Care Unit) and/or disease resolution., Materials and Methods: In 135 consecutive patients with SARS-CoV-2 infection, MR-proADM was measured in EDTA-K2 plasma samples using B.R.A.H.M.S. KRYPTOR® COMPACT Plus method (Thermo Fisher Scientific, Hennigsdorf, Germany) RESULTS: Patients were subdivided into three groups based on their MR-proADM value (nmol/L): 1 (n = 20, MR-proADM ≤ 0.55); 2 (n = 82, 0.55 < MR-proADM ≤ 1.50); 3 (n = 33, MR-proADM > 1.50). The higher the MR-proADM value, the greater the patients' age, the more frequent the occurrence of pneumonia, the requiring of more aggressive treatment, the longer the hospitalization and the more frequent a fatal event. Significant differences were found between the three groups for MR-proADM, White-blood cell count, Neutrophil count, D-dimer, C-reactive Protein, Procalcitonin and hs-Troponin I. At logistic regression,it was found that MR-proADM and Log
10 D-dimer were the most significant predictors of adverse events., Conclusion: The findings made in the present study highlight the relevance of MR-proADM values in providing clinically useful information, particularly for stratifying COVID-19 patients according to the risk of a more severe form of disease and to the development of adverse events., (Copyright © 2021 Elsevier B.V. All rights reserved.)- Published
- 2021
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29. Associations of maternal bisphenol urine concentrations during pregnancy with neonatal metabolomic profiles.
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Blaauwendraad SM, Voerman E, Trasande L, Kannan K, Santos S, Ruijter GJG, Sol CM, Marchioro L, Shokry E, Koletzko B, Jaddoe VWV, and Gaillard R
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- Benzhydryl Compounds, Child, Chromatography, Liquid, Female, Humans, Metabolomics, Phenols, Phosphatidylcholines, Pregnancy, Prospective Studies, Tandem Mass Spectrometry, Fatty Acids, Nonesterified, Lysophosphatidylcholines
- Abstract
Background: Fetal exposure to bisphenols is associated with altered fetal growth, adverse birth outcomes and childhood cardio-metabolic risk factors. Metabolomics may serve as a tool to identify the mechanisms underlying these associations. We examined the associations of maternal bisphenol urinary concentrations in pregnancy with neonatal metabolite profiles from cord blood., Methods: In a population-based prospective cohort study among 225 mother-child pairs, maternal urinary bisphenol A, S and F concentrations in first, second and third trimester were measured. LC-MS/MS was used to determine neonatal concentrations of amino acids, non-esterified fatty acids (NEFA), phospholipids (PL), and carnitines in cord blood., Results: No associations of maternal total bisphenol concentrations with neonatal metabolite profiles were present. Higher maternal average BPA concentrations were associated with higher neonatal mono-unsaturated alkyl-lysophosphatidylcholine concentrations, whereas higher maternal average BPS was associated with lower neonatal overall and saturated alkyl-lysophosphatidylcholine (p-values < 0.05).Trimester-specific analyses showed that higher maternal BPA, BPS and BPF were associated with alterations in neonatal NEFA, diacyl-phosphatidylcholines, acyl-alkyl-phosphatidylcholines, alkyl-lysophosphatidylcholine, sphingomyelines and acyl-carnitines, with the strongest effects for third trimester maternal bisphenol and neonatal diacyl-phosphatidylcholine, sphingomyeline and acyl-carnitine metabolites (p-values < 0.05). Associations were not explained by maternal socio-demographic and lifestyle characteristics or birth characteristics., Discussion: Higher maternal bisphenol A, F and S concentrations in pregnancy are associated with alterations in neonatal metabolite profile, mainly in NEFA, PL and carnitines concentrations. These findings provide novel insight into potential mechanisms underlying associations of maternal bisphenol exposure during pregnancy with adverse offspring outcomes but need to be replicated among larger, diverse populations., (© 2021. The Author(s).)
- Published
- 2021
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30. Associations of maternal and fetal SCD-1 markers with infant anthropometry and maternal diet: Findings from the ROLO study.
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Marchioro L, Hellmuth C, Uhl O, Geraghty AA, O'Brien EC, Horan MK, Donnelly JM, Kirchberg FF, Koletzko B, and McAuliffe FM
- Subjects
- Adiposity, Adult, Age Factors, Anthropometry, Biomarkers blood, Child, Preschool, Female, Humans, Infant, Newborn, Longitudinal Studies, Pediatric Obesity blood, Pediatric Obesity enzymology, Pediatric Obesity physiopathology, Pregnancy, Randomized Controlled Trials as Topic, Risk Assessment, Risk Factors, Child Development, Diet, Fatty Acids blood, Fetal Blood enzymology, Maternal Nutritional Physiological Phenomena, Pediatric Obesity etiology, Prenatal Exposure Delayed Effects, Stearoyl-CoA Desaturase blood
- Abstract
Background: Elevated stearoyl-CoA desaturase 1 (SCD-1) activity showed associations with obesity in cross-sectional studies. In non-pregnant populations, nutrition regulates SCD-1 transcription and activity., Objective: To investigate the longitudinal associations of maternal and fetal SCD-1 activity markers with infant anthropometry up to 2 years of age, and to explore how selected dietary intakes modulate SCD-1 activity in pregnancy., Methods: As a secondary analysis from the ROLO intervention study, which was conducted in a population at risk for macrosomia, non-esterified fatty acids (NEFA) from maternal plasma at 13 and 28 weeks' gestation and in cord blood were measured via liquid-chromatography-mass-spectrometry. Fatty acid ratios 18:1/18:0 and 16:1/16:0 were used as markers for SCD-1 activity ('desaturation indices', DIs). Relationships of DIs with infant anthropometry up to 2 years of age and maternal dietary parameters during pregnancy were investigated using adjusted linear regression models and p-values correction for multiple testing., Results: 18:1/18:0, but not 16:1/16:0, was associated with measures of infant anthropometry at birth (maternal and fetal markers) and up to 2 years of age (maternal markers only). Dietary intakes did not show strong associations with 18:1/18:0, but 16:1/16:0 was associated with absolute and relative dietary intakes., Conclusions: In a population at risk for macrosomia, maternal SCD-1 activity measured via 18:1/18:0 was involved in the fetal programming of infant obesity, but could not be substantially modulated by short-term diet in pregnancy., Clinical Trial Registration: ISRCTN Registration number: ISRCTN54392969 (http://www.isrctn.com/ISRCTN54392969)., (Copyright © 2019. Published by Elsevier Ltd.)
- Published
- 2020
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31. How short is too short? A randomised controlled trial evaluating short-term existential behavioural therapy for informal caregivers of palliative patients.
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Kühnel MB, Marchioro L, Deffner V, Bausewein C, Seidl H, Siebert S, and Fegg M
- Subjects
- Female, Humans, Male, Middle Aged, Palliative Care, Psychological Distress, Time, Behavior Therapy methods, Behavior Therapy standards, Behavior Therapy statistics & numerical data, Caregivers psychology, Caregivers statistics & numerical data, Depression therapy, Quality of Life
- Abstract
Background: Informal caregivers of palliative patients show higher levels of depression and distress compared with the general population. Fegg's (2013) existential behavioural therapy was shortened to two individual 1-h sessions (short-term existential behavioural therapy)., Aim: Testing the effectiveness of sEBT on psychological symptoms of informal caregivers in comparison with active control., Design: Randomised controlled trial., Setting/participants: Informal caregivers of palliative in-patients., Methods: The primary outcome was depression; secondary outcomes were anxiety, subjective distress and minor mental disorders, positive and negative affect, satisfaction with life, quality of life and direct health care costs. General linear mixed models allow several measurements per participant and change over time. Reasons for declining the intervention were investigated by Rosenstock's Health Belief Model., Results: Overall inclusion rate was 41.0%. Data of 157 caregivers were available (63.1% females; mean age: 54.6 years, standard deviation (SD): 14.1); 127 participants were included in the main analysis. Participation in sEBT or active control was not significantly associated with post-treatment depression. Outcomes showed prevailingly significant association with time of investigation. Self-efficacy, scepticism of benefit of the intervention, belief of better coping alone and support by family and friends were significant factors in declining participation in the randomised controlled trial., Conclusion: Inclusion rate was tripled compared with a previously evaluated longer EBT group intervention. By shortening the intervention, inclusion rate was traded for effectiveness and the intervention could not impact caregivers' psychological state. Early integration of sEBT and combination of individual and group setting and further study of the optimal length for caregiver interventions are suggested.
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- 2020
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32. Caesarean section, but not induction of labour, is associated with major changes in cord blood metabolome.
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Marchioro L, Shokry E, Geraghty AA, O'Brien EC, Uhl O, Koletzko B, and McAuliffe FM
- Subjects
- Adult, Child Development, Child, Preschool, Fatty Acids, Nonesterified blood, Female, Gas Chromatography-Mass Spectrometry, Humans, Infant, Infant, Newborn, Male, Metabolic Networks and Pathways, Phospholipids blood, Pregnancy, Cesarean Section adverse effects, Fetal Blood chemistry, Labor, Induced adverse effects, Metabolome
- Abstract
The physiology of how prelabour caesarean section (PCS) and induction of labour (IOL) in comparison to spontaneous vaginal delivery (SVD) has not been fully clarified yet. We measured 201 cord blood (CB) phospholipids and energy metabolites via LC/MS-MS in 109 newborns from the ROLO Kids study; metabolites were compared across the three parturition groups via linear mixed models with correction for multiple testing. In comparison to SVD, PCS babies had lower non-esterified fatty acids (NEFA), including sum of NEFA (p < 0.001), and trends for lower acylcarnitines. The lack of hormonal stimuli, especially catecholamines and cortisol, may underlie the metabolic changes involving gluconeogenesis from fatty acid oxidation (FAO) in PCS born infants. IOL and SVD infants showed no significant differences in metabolites, but ratios estimating carnitine palmitoyltrasferase 1 activity (precursor for FAO) were slightly higher in IOL than in SVD. Thus, IOL does not induce metabolic disadvantage when compared to SVD, though post-natal gluconeogenesis might start earlier due to the artificial solicitation in IOL. These data shed light on the physiology of parturition and may contribute to understand how mode of delivery might modulate future metabolic risks.
- Published
- 2019
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33. Transgenerational cycle of obesity and diabetes: investigating possible metabolic precursors in cord blood from the PREOBE study.
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Shokry E, Marchioro L, Uhl O, Bermúdez MG, García-Santos JA, Segura MT, Campoy C, and Koletzko B
- Subjects
- Adult, Birth Weight physiology, Body Mass Index, Case-Control Studies, Cohort Effect, Family Characteristics, Fatty Acids, Nonesterified analysis, Fatty Acids, Nonesterified blood, Female, Fetal Blood chemistry, Humans, Infant, Infant, Newborn, Male, Metabolomics instrumentation, Metabolomics methods, Middle Aged, Overweight complications, Pregnancy, Prenatal Exposure Delayed Effects blood, Prenatal Exposure Delayed Effects diagnosis, Adult Children statistics & numerical data, Child of Impaired Parents statistics & numerical data, Diabetes, Gestational blood, Diabetes, Gestational metabolism, Fetal Blood metabolism, Metabolome, Obesity blood, Obesity complications, Obesity diagnosis, Prenatal Exposure Delayed Effects metabolism
- Abstract
Aims: Offspring of mothers suffering from obesity and/or gestational diabetes mellitus (GDM) were reported to be at risk of higher birth weight (BW), later obesity and diabetes. We hypothesize that infant anthropometry changes related to maternal pathological status are due to dysregulated infant metabolism., Methods: First, we inspected differences in BMI z-scores (z-BMI) between three infant groups: born to normal weight (NW; n = 49), overweight/obese (OV/OB; n = 40) and GDM mothers (n = 27) at birth and 1 year. Then, we inspected associations between cord blood metabolites and 1-year Δ z-BMI in the three infant groups at birth and 1 year., Results: No statistically significant difference was detected in z-BMI between the study groups at birth; however, GDM was associated with heavier infants at 1 year. Regarding the associations between the metabolites and z-BMI, phospholipids, especially those containing polyunsaturated fatty acids, were the species most impacted by the maternal metabolic status, since numerous phosphatidylcholines-PUFA were positively associated with z-BMI in NW but negatively in OV/OB and GDM groups at birth. Conversely, the sum of lysophosphatidylcholines was only positively associated with z-BMI in NW at birth but of no relation in the other two groups. At 1 year, most of the associations seen at birth were reversed in NW and lost in OV/OB and GDM groups. In the NW group, PC-PUFA were found to be negatively associated with Δ z-BMI at 1 year in addition to some medium-chain acylcarnitines, tricarboxylic acid metabolites, Asp and Asn-to-Asp ratio. In OV/OB and GDM groups, the non-esterified fatty acid (NEFA26:0) and His correlated with Δ z-BMI at 1 year in negative and positive directions, respectively., Conclusions: GDM was associated with overweight in offspring at 1 year, independent of the BW with lack of evidence on existing correlation of this finding with metabolic alterations detected in cord blood metabolome. Associations were found between cord blood metabolites and infant anthropometry at birth and were influenced by maternal OB and GDM. However, an extension of the findings monitored at birth among the three groups was not detected longitudinally showing a lack of predictive power of cord blood metabolome for later development at least 1 year.
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- 2019
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34. Investigation of the impact of birth by cesarean section on fetal and maternal metabolism.
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Shokry E, Marchioro L, Uhl O, Bermúdez MG, García-Santos JA, Segura MT, Campoy C, and Koletzko B
- Subjects
- Adult, Cesarean Section statistics & numerical data, Chromatography, Liquid, Delivery, Obstetric methods, Fatty Acids, Nonesterified blood, Fatty Acids, Nonesterified metabolism, Female, Humans, Lipoproteins, HDL blood, Parturition, Phospholipids blood, Pregnancy, Prenatal Care, Spain, Tandem Mass Spectrometry, Cesarean Section adverse effects, Delivery, Obstetric statistics & numerical data, Fetal Blood metabolism, Fetus blood supply, Lipoproteins blood, Metabolomics
- Abstract
Purpose: Elective cesarean section (CS) was related to long-term adverse health effects in the offspring, but little is known about underlying mechanisms. Our study investigates the metabolic changes in both maternal and cord blood associated with CS in comparison to vaginal delivery (VD) to explore potential causal pathways., Methods: Samples obtained from PREOBE study participants were subjected to LC-MS/MS-targeted metabolomics comprising > 200 metabolites., Results: Elective CS showed an impact on both maternal and cord blood metabolomes. In maternal blood, the CS group showed lower levels of phospholipids (PL), principally ether-linked phosphatidylcholines (aaPC), pyruvic acid, branched chain keto-acids (BCKA), and other gluconeogenic substrates, but since the CS group showed different HDL levels in comparison to the VD group, we could not exclude contribution of the latter in the findings. In cord blood, the most remarkable finding in the CS group was the high levels of Cys; conversely, the lower levels of non-esterified fatty acids (NEFA), some tricarboxylic acid (TCA) cycle metabolites, gluconeogenic substrates, markers of β-oxidation, and the sum of hexoses were lower in CS-born babies in addition to tendentially lower levels of PL., Conclusions: We speculate that lower levels of maternal and fetal corticosteroids in CS, due to less stressful condition, cause metabolic perturbations at birth initiating future negative health outcomes. This further supports the early programming hypothesis.
- Published
- 2019
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35. Effect of a low glycaemic index diet during pregnancy on maternal and cord blood metabolomic profiles: results from the ROLO randomized controlled trial.
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Marchioro L, Geraghty AA, Uhl O, Shokry E, O'Brien EC, Koletzko B, and McAuliffe FM
- Abstract
Background: Elevated post-prandial blood glucose during pregnancy has been associated with adverse pregnancy and offspring outcomes, such as maternal gestational diabetes and excessive foetal growth. The ROLO Study is a randomized controlled trial (RCT) investigating the effect of a low glycaemic index (GI) diet in pregnancy to prevent foetal macrosomia (birth weight > 4000 g). We described the impact of a low-GI diet on the maternal and feto-placental unit metabolism by studying how the ROLO intervention affected maternal and cord blood metabolomes., Methods: Fasting maternal plasma samples pre- and post-intervention of 51 pregnant women and 132 cord blood samples were measured with a targeted metabolomics approach using liquid-chromatography coupled to tandem mass spectrometry. The differences between RCT groups were explored via multivariate models with covariates correction. Significance was set at Bonferroni-corrected level of 0.05., Results: A total of 262 metabolites species, sums and ratios were investigated. While no metabolite reached statistical significance after Bonferroni correction, many maternal phospholipids and acylcarnitines were elevated in the intervention group at uncorrected 0.05 alpha level. Most species contained saturated and monounsaturated fatty acid chains with 16 or 18 carbon atoms. In cord blood, no differences were identified between RCT groups., Conclusions: A low-GI diet in pregnancy was associated with a trend to modest but consistent changes in maternal lipid and fatty acid metabolism. The intervention seemed not to affect foetal metabolism. Our exploratory findings may be used to direct further investigations about low GI diets before and during pregnancy, to improve patient care for pre-conceptional and pregnant women with lipid dysregulations and potentially modulate the offspring's risk for future metabolic diseases., Trial Registration: Current Controlled Trials ISRCTN54392969., Competing Interests: Competing interestsThe authors declare no conflicts of interest i.e. circumstances that involve the risk that the professional judgment or acts of primary interest may be unduly influenced by a secondary interest.
- Published
- 2019
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36. Impact of maternal BMI and gestational diabetes mellitus on maternal and cord blood metabolome: results from the PREOBE cohort study.
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Shokry E, Marchioro L, Uhl O, Bermúdez MG, García-Santos JA, Segura MT, Campoy C, and Koletzko B
- Subjects
- Adult, Chromatography, Liquid, Cohort Studies, Fatty Acids metabolism, Fatty Acids, Nonesterified metabolism, Female, Humans, Infant, Newborn, Male, Obesity complications, Obesity metabolism, Phospholipids metabolism, Pregnancy, Pregnancy Complications metabolism, Prenatal Exposure Delayed Effects metabolism, Risk Factors, Tandem Mass Spectrometry, Body Mass Index, Diabetes, Gestational metabolism, Fetal Blood metabolism, Metabolome
- Abstract
Aims: Maternal obesity and gestational diabetes mellitus (GDM) were frequently reported to be risk factors for obesity and diabetes in offspring. Our goal was to study the impact of maternal prepregnancy BMI (pBMI) and GDM on both maternal and cord blood metabolic profiles., Methods: We used LC-MS/MS to measure 201 metabolites comprising phospholipids (PL), amino acids, non-esterified fatty acids (NEFA), organic acids, acyl carnitines (AC), and Krebs cycle metabolites in maternal plasma at delivery and cord plasma obtained from 325 PREOBE study participants., Results: Several metabolites were associated with pBMI/GDM in both maternal and cord blood (p < 0.05), while others were specific to either blood sources. BMI was positively associated with leucine, isoleucine, and inflammation markers in both mother and offspring, while β-hydroxybutyric acid was positively associated only in cord blood. GDM showed elevated levels of sum of hexoses, a characteristic finding in both maternal and cord blood. Uniquely in cord blood of offspring born to GDM mothers, free carnitine was significantly lower with the same tendency observed for AC, long-chain NEFA, PL, specific Krebs cycle metabolites, and β-oxidation markers., Conclusions: Maternal BMI and GDM are associated with maternal and cord blood metabolites supporting the hypothesis of transgenerational cycle of obesity and diabetes.
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- 2019
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37. Early Programming of Obesity Throughout the Life Course: A Metabolomics Perspective.
- Author
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Rauschert S, Kirchberg FF, Marchioro L, Koletzko B, Hellmuth C, and Uhl O
- Subjects
- Amino Acids, Aromatic metabolism, Amino Acids, Branched-Chain metabolism, Biomarkers metabolism, Fatty Acids, Unsaturated metabolism, Female, Humans, Lysophosphatidylcholines metabolism, Male, Risk Factors, Sex Factors, Sexual Maturation, Sphingomyelins metabolism, Metabolome physiology, Metabolomics, Obesity etiology
- Abstract
Background: Over the last decades, research on early life risk factors for obesity and its comorbidities in early life has gained attention within the field of developmental origins of health and diseases. Metabolomics studies that are trying to find early life biomarker and intervention targets for the early development of obesity and associated cardiovascular diseases could help break the inter-generational cycle of obesity., Summary: Metabolomics studies in the field of early programming are scarce and causality is lacking at this stage, as most of the studies are cross-sectional. The main metabolites in the focus of obesity are branched-chain and aromatic amino acids, long-chain polyunsaturated fatty acids, lysophosphatidylcholines, and sphingomyelins. Sex and puberty have not been considered in most of the biomarker studies, but show differences in the metabolite associations to obesity. Key Messages: There is still a lot unknown about the associations between early programming exposures, metabolite concentrations, and the development of obesity. The few studies focusing on this topic find similar metabolite classes in the same age groups being associated with rapid early growth or obesity; but due to differences in the methodological and statistical approaches, the single species often differ. Therefore, more research, preferably with standardized approaches, is needed., (© 2017 S. Karger AG, Basel.)
- Published
- 2017
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38. Sleep-deprivation reduces NK cell number and function mediated by β-adrenergic signalling.
- Author
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De Lorenzo BH, de Oliveira Marchioro L, Greco CR, and Suchecki D
- Subjects
- Animals, Disease Models, Animal, Killer Cells, Natural immunology, Killer Cells, Natural metabolism, Male, Melanoma, Experimental immunology, Melanoma, Experimental metabolism, Melanoma, Experimental pathology, Mice, Mice, Inbred C57BL, Natural Killer T-Cells immunology, Natural Killer T-Cells pathology, Stress, Physiological immunology, Killer Cells, Natural pathology, Receptors, Adrenergic, beta immunology, Sleep Deprivation immunology, Sleep Deprivation pathology
- Abstract
Reduction of sleep time triggers a stress response, leading to augmented levels of glucocorticoids and adrenaline. These hormones regulate components of the innate immune system such as natural killer (NK) and NKT cells. In the present study, we sought to investigate whether and how stress hormones could alter the population and function of NK and NKT cells of mice submitted to different lengths of paradoxical sleep deprivation (PSD, from 24 to 72 h). Results showed that 72h of PSD decreased not only NK and NKT cell counts, but also their cytotoxic activity against B16F10 melanoma cells in vitro. Propranolol treatment during PSD reversed these effects, indicating a major inhibitory role of beta-adrenergic receptors (β-AR) on NK cells function. Moreover, both corticosterone plasma levels and expression of beta 2-adrenergic receptors (β2-AR) in NK cells increased by 48 h of PSD. In vitro incubation of NK cells with dexamethasone augmented the level of β2-AR in the cell surface, suggesting that glucocorticoids could induce β2-AR expression. In summary, we propose that reduction of NK and NKT cell number and cytotoxic activity appears to be mediated by glucocorticoids-induced increased expression of β2-AR in these cells., (Copyright © 2015 Elsevier Ltd. All rights reserved.)
- Published
- 2015
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39. Assessment of alcohol consumption in liver transplant candidates and recipients: the best combination of the tools available.
- Author
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Piano S, Marchioro L, Gola E, Rosi S, Morando F, Cavallin M, Sticca A, Fasolato S, Forza G, Chiara Frigo A, Plebani M, Zanus G, Cillo U, Gatta A, and Angeli P
- Subjects
- Aged, Alcoholism complications, Alcoholism diagnosis, Biomarkers blood, Biomarkers urine, Ethanol blood, Ethanol urine, Female, Glucuronates urine, Humans, Male, Middle Aged, Odds Ratio, Prospective Studies, ROC Curve, Recurrence, Transferrin analogs & derivatives, Transferrin analysis, Alcohol Drinking, End Stage Liver Disease complications, End Stage Liver Disease therapy, Liver Transplantation
- Abstract
The detection of alcohol consumption in liver transplant candidates (LTCs) and liver transplant recipients (LTRs) is required to enable a proper assessment of transplant eligibility and early management of alcohol relapse, respectively. In this clinical setting, urinary ethyl glucuronide (uEtG), the Alcohol Use Disorders Identification Test for Alcohol Consumption (AUDIT-c), serum ethanol, urinary ethanol, carbohydrate-deficient transferrin (CDT), and other indirect markers of alcohol consumption were evaluated and compared prospectively in 121 LTCs and LTRs. Alcohol consumption was diagnosed when AUDIT-c results were positive or it was confirmed by a patient's history in response to abnormal results. Alcohol consumption was found in 30.6% of the patients. uEtG was found to be the strongest marker of alcohol consumption (odds ratio = 414.5, P < 0.001) and provided a more accurate prediction rate of alcohol consumption [area under receiving operating characteristic (ROC) curve = 0.94] than CDT (area under ROC curve = 0.63, P < 0.001) and AUDIT-c (area under ROC curve = 0.73, P < 0.001). The combination of uEtG and AUDIT-c showed higher accuracy in detecting alcohol consumption in comparison with the combination of CDT and AUDIT-c (area under ROC curve = 0.98 versus 0.80, P < 0.001). Furthermore, uEtG was the most useful marker for detecting alcohol consumption in patients with negative AUDIT-c results. In conclusion, the combination of AUDIT-c and uEtG improves the detection of alcohol consumption in LTCs and LTRs. Therefore, they should be used routinely for these patients., (© 2014 American Association for the Study of Liver Diseases.)
- Published
- 2014
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40. Laboratory medicine and sports: between Scylla and Charybdis.
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Lippi G, Banfi G, Botrè F, de la Torre X, De Vita F, Gomez-Cabrera MC, Maffulli N, Marchioro L, Pacifici R, Sanchis-Gomar F, Schena F, and Plebani M
- Subjects
- Doping in Sports, Humans, Exercise, Medical Laboratory Science methods, Sports, Sports Medicine methods
- Abstract
Laboratory medicine is complex and contributes to the diagnosis, therapeutic monitoring and follow-up of acquired and inherited human disorders. The regular practice of physical exercise provides important benefits in heath and disease and sports medicine is thereby receiving growing focus from almost each and every clinical discipline, including laboratory medicine. Sport-laboratory medicine is a relatively innovative branch of laboratory science, which can provide valuable contributions to the diagnosis and follow-up of athletic injuries, and which is acquiring a growing clinical significance to support biomechanics and identify novel genomics and "exercisenomics" patterns that can help identify specific athlete's tendency towards certain types of sport traumas and injuries. Laboratory medicine can also provide sport physicians and coaches with valuable clues about personal inclination towards a certain sport, health status, fitness and nutritional deficiencies of professional, elite and recreational athletes in order to enable a better and earlier prediction of sport injuries, overreaching and overtraining. Finally, the wide armamentarium of laboratory tests represents the milestone for identifying cheating athletes in the strenuous fight against doping in sports.
- Published
- 2012
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41. Autoantibodies against oxidized LDLs and atherosclerosis in type 2 diabetes.
- Author
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Piarulli F, Lapolla A, Sartore G, Rossetti C, Bax G, Noale M, Minicuci N, Fiore C, Marchioro L, Manzato E, and Fedele D
- Subjects
- Age of Onset, Arteriosclerosis blood, Biomarkers blood, Carotid Artery, Common pathology, Carotid Stenosis blood, Carotid Stenosis pathology, Cholesterol blood, Diabetes Mellitus, Type 2 blood, Diabetic Angiopathies immunology, Diabetic Angiopathies pathology, Female, Humans, Lipoproteins blood, Male, Middle Aged, Smoking, Time Factors, Tunica Intima pathology, Tunica Media pathology, Arteriosclerosis immunology, Autoantibodies blood, Carotid Stenosis immunology, Diabetes Mellitus, Type 2 immunology, Lipoproteins, LDL immunology
- Abstract
Objective: The aim of our study was to examine, in type 2 diabetic patients, the relationship between autoantibodies against oxidatively modified LDL (oxLDL Ab) and two indexes of atherosclerosis, intimal-medial thickness of the common carotid artery (CCA-IMT), which reflects early atherosclerosis, and the ankle-brachial index (ABI), which reflects advanced atherosclerosis., Research Design and Methods: Thirty newly diagnosed type 2 diabetic patients, 30 type 2 diabetic patients with long duration of disease, and 56 control subjects were studied. To detect oxLDL Ab, the ImmunoLisa Anti-oxLDL Antibody ELISA was used. ABI was estimated at rest by strain-gauge plethysmography. Carotid B-mode imaging was performed on a high-resolution imaging system (ATL HDI 5000)., Results: In patients with long duration of disease, IgG oxLDL Ab were significantly higher and ABI significantly lower compared with the other two groups. We found a correlation between IgG oxLDL Ab and CCA-IMT in all diabetic patients. A significant inverse correlation between IgG oxLDL Ab and ABI only in patients with long duration of disease was seen, demonstrating a close relationship between these autoantibodies and advanced atherosclerosis., Conclusions: IgG OxLDL Ab may be markers of the advanced phase of the atherosclerotic process and the response of the immunological system to the oxLDL, which are present within atherosclerotic lesions.
- Published
- 2005
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42. Alcohol is an important co-factor for both steatosis and fibrosis in Northern Italian patients with chronic hepatitis C.
- Author
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Fabris P, Floreani A, Carlotto A, Giordani MT, Baldo V, Stecca C, Marchioro L, Tramarin A, Bertin T, Negro F, and de Lalla F
- Subjects
- Adolescent, Adult, Aged, Alcoholism complications, Fatty Liver pathology, Fatty Liver virology, Female, Genotype, Hepacivirus genetics, Hepatitis C, Chronic pathology, Hepatitis C, Chronic virology, Humans, Italy, Liver Cirrhosis virology, Logistic Models, Male, Medical Records, Middle Aged, Multivariate Analysis, Alcohol Drinking adverse effects, Fatty Liver etiology, Hepatitis C, Chronic complications, Liver Cirrhosis etiology
- Abstract
Background/aims: Steatosis in patients with chronic hepatitis C (CHC) may be the result of both viral and host factors. To evaluate: (1) the relationship between steatosis and either host or viral factors; (2) the correlation between steatosis and fibrosis in patients with CHC., Methods: A consecutive series of 349 patients were evaluated for steatosis. At liver biopsy, patients were tested for virological, and laboratory analysis and questioned for alcohol consumption., Results: Logistic regression analysis demonstrated that steatosis was independently associated with genotype 3a (odds ratio, OR 3.5), alcohol intake at the time of biopsy (OR 2.6) and age >35 years (OR 2.7). In multivariate analysis the presence of fibrosis was associated with past alcohol abuse (OR 3.7), and age older than 44 years (OR 2.2). Overall, a weak correlation was found between grade of steatosis and fibrosis score (r=0.861, P=0.05), which disappeared excluding patients without past or current alcohol intake. A direct correlation emerged between grade of steatosis and both 'grading' and 'staging' only in patients with genotypes other than 3a., Conclusions: Genotype 3a is the main risk factor for steatosis in patients with CHC. The grade of steatosis correlated with both grading and staging only in patients with genotypes other than 3a and this relationship is strictly linked to alcohol consumption.
- Published
- 2004
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43. Amphetamines and 3,4-methylendioxymetamphetamine (MDMA): evaluation of KIMS (kinetic interaction of microparticles in solution) assay at two cut-off levels.
- Author
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Marchioro L, Bassetto F, Dall'Olio G, Tedeschi L, Castagna F, and Plebani M
- Subjects
- 3,4-Methylenedioxyamphetamine urine, Adolescent, Adult, Chromatography, High Pressure Liquid, Clinical Chemistry Tests statistics & numerical data, Enzyme Multiplied Immunoassay Technique statistics & numerical data, Female, Gas Chromatography-Mass Spectrometry, Humans, Immunoassay statistics & numerical data, Male, Methamphetamine urine, Middle Aged, Nanostructures, Predictive Value of Tests, Sensitivity and Specificity, Substance-Related Disorders diagnosis, Substance-Related Disorders urine, Amphetamines urine, Clinical Chemistry Tests methods, Immunoassay methods, N-Methyl-3,4-methylenedioxyamphetamine urine
- Abstract
Two screening methods for the assay of amphetamines and their derivatives have been applied to the same analytical instrument for their evaluation. In addition to an assay at a cut-off of 1000 microg/l, a new specific reagent was evaluated for an ultra-sensitive assay of amphetamines and 3,4-methylendioxymetamphetamine with a cut-off of 300 microg/l. The assay confirmation was performed using high-performance liquid chromatography and gas chromatography/ mass spectrometry techniques. The results were positive for both screening methods, confirming the efficacy of two simultaneous methods with different cut-off levels.
- Published
- 2004
- Full Text
- View/download PDF
44. Meningeal melanocytoma: a rare lesion of the central nervous system.
- Author
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Gardiman M, Altavilla G, Marchioro L, Boscolo L, Alessio L, and Piazza M
- Subjects
- Adult, Humans, Immunohistochemistry, Male, Melanoma pathology, Meningeal Neoplasms pathology
- Abstract
Two cases of meningeal melanocytoma, both localized in the cerebellopontine angle, were studied. The patients underwent surgical excision, and in one case there was a recurrence. All the pathologic criteria of melanocytoma were fulfilled by the 2 reported cases. Immunohistochemical stainings were very important ancillary tools in the differential diagnosis with other pigmented meningeal tumors.
- Published
- 1996
- Full Text
- View/download PDF
45. Metastasis to intracranial meningioma as first clinical manifestation of occult primary lung carcinoma.
- Author
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Gardiman M, Altavilla G, Marchioro L, Alessio L, Parenti A, and Piazza M
- Subjects
- Humans, Immunohistochemistry, Male, Meningeal Neoplasms pathology, Meningioma pathology, Middle Aged, Lung Neoplasms pathology, Meningeal Neoplasms secondary, Meningioma secondary
- Abstract
Metastasis to an intracranial meningioma was the first clinical manifestation of an occult primary bronchogenic carcinoma. The principles of diagnosis on morphologic and immunohistochemical findings are discussed, particularly as regards the differential diagnosis between a metastasis to meningioma and microcystic or secretory meningioma.
- Published
- 1996
46. [Biohumeral markers in the diagnosis of pancreatic carcinoma].
- Author
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Panucci A, Del Favero G, Falmis C, Brosolo P, Basso D, Pedrazzoli S, Baccaglini U, Marchioro L, Bonvilini P, and Naccarato R
- Subjects
- Antigens, Neoplasm analysis, Antigens, Tumor-Associated, Carbohydrate, Carcinoembryonic Antigen analysis, Diagnosis, Differential, Ferritins blood, Humans, Pancreatic Juice analysis, Pancreatitis diagnosis, Peptides analysis, Ribonucleases blood, Tissue Polypeptide Antigen, Trypsin metabolism, Pancreatic Neoplasms diagnosis
- Published
- 1985
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