22 results on '"Marchiol-Fournigault C"'
Search Results
2. A member of the tetra spans transmembrane protein superfamily is recognized by a monoclonal antibody raised against an HLA class I-deficient, lymphokine-activated killer-susceptible, B lymphocyte line. Cloning and preliminary functional studies
- Author
-
María Luisa Gil, Vita, N., Lebel-Binay, S., Miloux, B., Chalon, P., Kaghad, M., Marchiol-Fournigault, C., Conjeaud, H., Caput, D., Ferrara, P., Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Matière et Systèmes Complexes (MSC (UMR_7057)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), and QUILLET-MARY, Anne
- Subjects
[SDV.IMM] Life Sciences [q-bio]/Immunology ,Immunology ,Molecular Sequence Data ,Dose-Response Relationship, Immunologic ,Fluorescent Antibody Technique ,Lymphocyte Activation ,Cell Line ,Sequence Homology, Nucleic Acid ,Immunology and Allergy ,Humans ,Amino Acid Sequence ,Killer Cells, Lymphokine-Activated ,B-Lymphocytes ,Base Sequence ,Tumor Necrosis Factor-alpha ,Histocompatibility Antigens Class II ,Antibodies, Monoclonal ,Flow Cytometry ,Burkitt Lymphoma ,Up-Regulation ,Gene Expression Regulation ,Antigens, Surface ,[SDV.IMM]Life Sciences [q-bio]/Immunology ,Calcium ,Interleukin-4 ,Signal Transduction - Abstract
The IA4 mAb was identified among a series of antibodies raised in BALB/c mice after immunization against a HLA class I-deficient, lymphokine-activated killer (LAK)-susceptible EBV-B lymphocyte line. The IA4 antibody was selected because of its high expression, in the range of 10(5) to 25 x 10(5) sites/cell, on several B lymphocyte lines (EBV-transformed or Burkitt) and monocytic lines such as HL60 and U937, and because its expression was correlated with both target susceptibility to LAK lysis and reduced expression of HLA class I surface Ag on two pairs of EBV-B-transformed cell lines (721/721.134 and MM/10F2). Despite the strategy followed to raise the mAb and the correlation mentioned above, no direct role of the IA4 molecules in LAK susceptibility has been established, since the IA4 molecule is poorly expressed on the sensitive targets Daudi and K562; moreover, the IA4 antibody did not affect reproducibly the in vitro killing of positive target cells by LAK effectors. The IA4 antibody was poorly immunoprecipitating and the surface molecule recognized was identified by gene cloning following an expression strategy using a U937 cDNA library transfected in COS cells, and a screening strategy based on membrane expression of IA4 molecule. The IA4 cDNA is virtually identical to "R2," a mRNA species previously identified in activated human T cells by subtractive hybridization. The IA4 cDNA contains an open reading frame coding for a protein 267 amino acids long with four potential transmembrane domains and one large external hydrophilic domain of about 110 amino acids, possibly glycosylated. The encoded protein belongs to a family of surface molecules, the tetra spans transmembrane protein superfamily, all displaying the four transmembrane domains, expressed on various cell types including lymphocytes (CD9, CD37, CD53, TAPA-1), melanoma cells (ME491), and intestinal cells (CO-029). These molecules have been reported to be involved in cell activation and cell death. Surprisingly, the Schistosoma mansoni Ag Sm23 displays significant homologies with this family. The IA4 molecule is a widely distributed surface marker expressed on circulating lymphocytes and monocytes, newborn thymocytes, and the cell lines mentioned above. The IA4 molecule expression is up-regulated upon cell activation. Weakly expressed on resting peripheral T and B lymphocytes and large granular lymphocytes (NK), its expression roughly doubles after activation by PHA, staphylococcus aureus Cowan I, and IL-2, respectively.(ABSTRACT TRUNCATED AT 400 WORDS)
- Published
- 1992
3. Évaluation des arthrites expérimentales par échographie haute résolution et Doppler chez la souris
- Author
-
Clavel, G, primary, Bessis, N, additional, Marchiol-Fournigault, C, additional, Renault, G, additional, Fradelizi, D, additional, and Boissier, MC, additional
- Published
- 2007
- Full Text
- View/download PDF
4. G.P.6.14 Cardiac characterization of mdx mice using high-resolution echocardiography
- Author
-
Fayssoil, A., primary, Renault, G., additional, Marchiol-Fournigault, C., additional, Fradelizi, D., additional, Rosier-Montus, M., additional, Richard, I., additional, and Fougerousse, F., additional
- Published
- 2007
- Full Text
- View/download PDF
5. L’échographie haute résolution de la souris
- Author
-
Renault, G., primary, Bonnin, P., additional, Marchiol-Fournigault, C., additional, Gregoire, J.M., additional, Serrière, S., additional, Richard, B., additional, and Fradelizi, D., additional
- Published
- 2006
- Full Text
- View/download PDF
6. A member of the tetra spans transmembrane protein superfamily is recognized by a monoclonal antibody raised against an HLA class I-deficient, lymphokine-activated killer-susceptible, B lymphocyte line. Cloning and preliminary functional studies.
- Author
-
Gil, M L, primary, Vita, N, additional, Lebel-Binay, S, additional, Miloux, B, additional, Chalon, P, additional, Kaghad, M, additional, Marchiol-Fournigault, C, additional, Conjeaud, H, additional, Caput, D, additional, and Ferrara, P, additional
- Published
- 1992
- Full Text
- View/download PDF
7. Generation of lymphokine-activated killer cells: synergy between tumor necrosis factor and interleukin 2.
- Author
-
Chouaib, S, Bertoglio, J, Blay, J Y, Marchiol-Fournigault, C, and Fradelizi, D
- Abstract
Large granular lymphocytes (LGL) can be activated by interleukin 2 (IL-2) to lymphokine-activated killers (LAK). The effect of tumor necrosis factor (TNF) on LAK generation was investigated. TNF was found to act synergistically with low concentrations of IL-2 (0.10-0.25 ng/ml), which were ineffective by themselves in inducing LAK activity, to promote the differentiation of LGL into non-major histocompatibility complex-restricted killers. When IL-2 was used at concentrations optimal for LAK generation, TNF did not further enhance this phenomenon. Specific binding of 125I-labeled TNF to LGL was increased by IL-2 stimulation. Scatchard analysis of TNF binding revealed the existence of two classes of binding sites with markedly different affinities (Kd values of 57 and 600 pM). We also demonstrated that the IL-2/TNF synergistic induction of LAK activity did not involve either IL-1 or interferon-gamma. This IL-2/TNF synergistic effect was blocked by anti-Tac antibodies. Immunofluorescence analysis revealed that IL-2/TNF selectively up-regulated Tac antigen expression on LAK precursors. Our results suggest a functional interaction between IL-2 and TNF on LAK precursors, which results in a reduction of the IL-2 concentration required for differentiation of LGL into LAK killers.
- Published
- 1988
- Full Text
- View/download PDF
8. Susceptibility of human target cells to NK/LAK cytotoxicity is inversely correlated with HLA A, -B expression
- Author
-
Quillet, A., Marchiol-Fournigault, C., Harel-Bellan, A., and didier fradelizi
9. Allogeneic T cell activation triggering by MHC class I antigens
- Author
-
Chouaib S, Armand Bensussan, Am, Termijtelen, Andreeff M, Marchiol-Fournigault C, Fradelizi D, and Dupont B
- Subjects
Cytotoxicity, Immunologic ,Male ,Immunology ,Antibodies, Monoclonal ,Lymphocyte Activation ,Tumor Necrosis Factor Receptor Superfamily, Member 7 ,HLA Antigens ,Antigens, Surface ,Immunology and Allergy ,Humans ,Interleukin-2 ,Female ,Interphase ,Interleukin-1 ,T-Lymphocytes, Cytotoxic - Abstract
The role of MHC-encoded class I molecules in allogeneic activation and proliferation of human T lymphocytes was investigated. The study was performed by using primary mixed culture of lymphocytes from MHC recombinant siblings identical for MHC class II Ag (DR, DP, DQ) and displaying MHC class I disparity. The results indicate that such allogeneic combination is sufficient to trigger early activation steps within responder T cells without promoting a significant proliferation. After MHC class I allosensitization, a significant proportion of cells entered the cell cycle (G0----G1). The stimulatory potential of MHC class I Ag was further stressed by the specific induction on responder cells of IL-2R (22% T cell activation Ag positive). Under the same experimental conditions, transferrin receptor expression and IL-2 activity were not detectable. This is consistent with the low T cell proliferation. Exogenous rIL-1 did not improve IL-2 production and the subsequent T cell proliferation indicating that these two events were not associated with a defective accessory cell function involving IL-1 release. MHC class I disparity can also prime precursor CTL to differentiate into IL-2-dependent functional MHC restricted cytotoxic T cells. Conversely IFN-gamma had no effect. Addition to the culture of W6/32, a mAb specifically directed against a monomorphic determinant on human class I HLA-A, -B, and -C Ag was able to block all these activation events. These data clearly indicate a role of HLA class I Ag involvement in the early events triggering allogeneic T cell activation.
10. Allogeneic T cell activation triggering by MHC class I antigens.
- Author
-
Chouaib, S, primary, Bensussan, A, additional, Termijtelen, A M, additional, Andreeff, M, additional, Marchiol-Fournigault, C, additional, Fradelizi, D, additional, and Dupont, B, additional
- Published
- 1988
- Full Text
- View/download PDF
11. High-protein-low-carbohydrate diet: deleterious metabolic and cardiovascular effects depend on age.
- Author
-
Bedarida T, Baron S, Vessieres E, Vibert F, Ayer A, Marchiol-Fournigault C, Henrion D, Paul JL, Noble F, Golmard JL, Beaudeux JL, Cottart CH, and Nivet-Antoine V
- Subjects
- Age Factors, Animals, Aorta pathology, Blood Glucose metabolism, Dietary Proteins adverse effects, Echocardiography, Glucose Intolerance metabolism, Lipid Metabolism, Mesenteric Arteries metabolism, Mesenteric Arteries pathology, Mice, Mice, Inbred C57BL, Myocardium pathology, Resistin blood, Triglycerides blood, Ventricular Dysfunction, Left metabolism, Aorta metabolism, Diet, Carbohydrate-Restricted adverse effects, Dietary Proteins administration & dosage, Glucose Intolerance etiology, Myocardium metabolism, Ventricular Dysfunction, Left etiology
- Abstract
High-protein-low-carbohydrate (HP-LC) diets have become widespread. Yet their deleterious consequences, especially on glucose metabolism and arteries, have already been underlined. Our previous study (2) has already shown glucose intolerance with major arterial dysfunction in very old mice subjected to an HP-LC diet. The hypothesis of this work was that this diet had an age-dependent deleterious metabolic and cardiovascular outcome. Two groups of mice, young and adult (3 and 6 mo old), were subjected for 12 wk to a standard or to an HP-LC diet. Glucose and lipid metabolism was studied. The cardiovascular system was explored from the functional stage with Doppler-echography to the molecular stage (arterial reactivity, mRNA, immunohistochemistry). Young mice did not exhibit any significant metabolic modification, whereas adult mice presented marked glucose intolerance associated with an increase in resistin and triglyceride levels. These metabolic disturbances were responsible for cardiovascular damages only in adult mice, with decreased aortic distensibility and left ventricle dysfunction. These seemed to be the consequence of arterial dysfunctions. Mesenteric arteries were the worst affected with a major oxidative stress, whereas aorta function seemed to be maintained with an appreciable role of cyclooxygenase-2 to preserve endothelial function. This study highlights for the first time the age-dependent deleterious effects of an HP-LC diet on metabolism, with glucose intolerance and lipid disorders and vascular (especially microvessels) and cardiac functions. This work shows that HP-LC lead to equivalent cardiovascular alterations, as observed in very old age, and underlines the danger of such diet., (Copyright © 2014 the American Physiological Society.)
- Published
- 2014
- Full Text
- View/download PDF
12. Cardiac Characterization of sgca-Null Mice Using High Resolution Echocardiography.
- Author
-
Fayssoil A, Renault G, Guerchet N, Marchiol-Fournigault C, Fougerousse F, and Richard I
- Abstract
Limb-girdle muscular dystrophy 2D (LGMD2D) is an inherited myogenic disorder belonging to the group of muscular dystrophies. Sgca-null mouse is a knock-out model of LGMD2D. Little is known about cardiac phenotype characterization in this model at different ages. We conducted a prospective study to characterize cardiac sgca-null mice phenotype using high resolution Doppler echocardiography at different ages. Conventional echocardiography was performed on anesthetised mice using a Vevo 770 (Visualsonics) with 30 MHz cardiac probe. Wild Type (WT) and sgca-null mice were scanned at 13, 15 and 17 months. From M-mode, we measured interventricular septal (IVS) wall thickness, posterior wall (PW) thickness, and end-left ventricular diameter in systolic and diastolic. From the above parameters, we calculated left ventricular (LV) shortening fraction (SF), LV ejection fraction (EF) and LV mass. At age 13 months, PW diastolic thickness was increased in sgca-null mice (0.89±0.14 mm vs 0.73±0.2 mm; P=0.020) and LV mass was higher in sgca-null mice (LV mass 205.2 mg vs 143 mg; P=0.001). We found also dilation of the LV (LVEDD: 4.84 mm vs 4.29 mm; P=0.019) in sgca-null mice. At age 15 months, dilation of the LV (LVEDD: 4.86 mm vs 4 mm; P=0.05) with an increase of the LV mass (165.7 mg vs 127.12; P=0.03) are found in sgca-null mice. At age 17 months, we found a decrease of the PW thickening (17% vs 30%; P=0.036). This work provides echocardiographic insights for the assessment of pharmaceutical therapies in sgca-null mice.
- Published
- 2013
- Full Text
- View/download PDF
13. Cardiac characterization of mdx mice using high-resolution doppler echocardiography.
- Author
-
Fayssoil A, Renault G, Guerchet N, Marchiol-Fournigault C, Fougerousse F, and Richard I
- Subjects
- Animals, Image Enhancement methods, Mice, Mice, Inbred C57BL, Mice, Inbred mdx, Reproducibility of Results, Sensitivity and Specificity, Algorithms, Cardiomyopathies diagnostic imaging, Echocardiography, Doppler methods, Image Interpretation, Computer-Assisted methods, Muscular Dystrophy, Duchenne diagnostic imaging, Ventricular Dysfunction, Left
- Abstract
Objectives: Duchenne muscular dystrophy is an X-linked neuromuscular disorder. The heart is traditionally involved, leading to heart failure. The mdx mouse is a natural animal model of the disease. We conducted a prospective study to analyze left ventricular (LV) function in mdx mice at different ages using high-resolution Doppler echocardiography., Methods: Echocardiography was performed with a 30-MHz cardiac probe. Wild-type and mdx mice were scanned at 10 and 12 months. We measured the interventricular septal wall thickness, posterior wall thickness, and LV diameter in systole and diastole. The LV shortening fraction, LV ejection fraction, and LV mass were then calculated., Results: At 10 months, the shortening fractions in mdx and wild-type mice were relatively close (29% ± 5% versus 25% ± 4%). We found a significant difference in the posterior wall thickness change (40% ± 12% in mdx versus 28% ± 10% in wild-type; P = .048). The LV mass/body weight ratio was higher in mdx than wild-type mice (3.67 ± 0.25 versus 3.39 ± 0.26; P = .05). At 12 months, the LV mass was elevated in mdx mice compared to wild-type (152 ±16 versus 135 ± 3.7 mg; P = .04). The diastolic posterior wall thickness change was decreased in mdx mice at 12 months compared to wild-type (21% ± 7% versus 33% ± 4%; P = .01). The LV ejection fraction was not statistically different between mdx and wild-type mice (50% ± 6% versus 54% ± 2%)., Conclusions: Ten-month-old mdx mice had a significantly higher posterior wall thickness than wild-type mice, but it was not significant at 12 months. In 12-month-old mdx mice, the posterior wall thickness change was significantly lower, and the LV mass was significantly higher. These findings indicate the role of LV function in the early stages of Duchenne muscular dystrophy.
- Published
- 2013
- Full Text
- View/download PDF
14. Dramatic efficacy improvement of a DC-based vaccine against AML by CD25 T cell depletion allowing the induction of a long-lasting T cell response.
- Author
-
Delluc S, Hachem P, Rusakiewicz S, Gaston A, Marchiol-Fournigault C, Tourneur L, Babchia N, Fradelizi D, Regnault A, Sang KH, Chiocchia G, and Buzyn A
- Subjects
- Animals, Antibodies, Monoclonal immunology, Female, Interleukin-2 Receptor alpha Subunit metabolism, Leukemia, Myeloid, Acute pathology, Lymphocyte Depletion, Mice, Mice, Inbred C57BL, Survival Rate, T-Lymphocytes pathology, Vaccination, Cancer Vaccines immunology, Dendritic Cells immunology, Leukemia, Myeloid, Acute immunology, Leukemia, Myeloid, Acute therapy, T-Lymphocytes immunology
- Abstract
Dendritic cell (DC)-based vaccination is a promising approach to enhance anti-tumor immunity that could be considered for acute myeloid leukemia (AML) patients with high-risk of relapse. Our purpose was to study the efficiency and to optimize the immunogenicity of a DC-based vaccine in a preclinical AML murine model. In this report, C57BL6 mice were vaccinated with DC pulsed with peptides eluted (EP) from the syngeneic C1498 myelomonocytic leukemic cell line in a prophylactic setting. In this model, a natural antileukemic immunity mediated by NK cells was observed in the control unloaded DC-vaccinated group. On the other hand, we showed that the cytotoxic antileukemic immune response induced by vaccination with eluted peptides pulsed-DC (DC/EP), in vitro and in vivo, was mainly mediated by CD4(+) T cells. Treatment with anti-CD25 antibody to deplete CD4(+) CD25(+) regulatory T cells before DC-vaccination dramatically improved the antileukemic immune response induced by immunization, and allowed the development of long-lasting immune responses that were tumor protective after a re-challenge with leukemic cells. Our results suggest that this approach could be successful against weakly immunogenic tumors such as AML, and could be translated in human.
- Published
- 2009
- Full Text
- View/download PDF
15. Identification of Quantitative Trait Loci responsible for embryonic lethality in mice assessed by ultrasonography.
- Author
-
Laissue P, Burgio G, l'Hôte D, Renault G, Marchiol-Fournigault C, Fradelizi D, Fellous M, Serres C, Montagutelli X, Monget P, and Vaiman D
- Subjects
- Animals, Female, Genetic Markers genetics, Male, Mice, Phenotype, Pregnancy, Ultrasonography, Embryo Loss diagnostic imaging, Embryo Loss genetics, Quantitative Trait Loci genetics
- Abstract
Recurrent Spontaneous Abortion (RSA) is a frequent pathology affecting 1 to 5% of couples. In approximately 50 % of cases, the aetiology is unknown suggesting a subtle interaction between genetic and environmental factors. Previous attempts to describe genetic factors using the candidate gene approach have been relatively unsuccessful due to the physiological, cellular and genetic complexity of mammalian reproduction. Indeed, fertility can be considered as a quantitative feature resulting from the interaction of genetic, epigenetic and environmental factors. Herein, we identified Quantitative Trait Loci (QTL) associated with diverse embryonic lethality phenotypes and the subsequent embryonic resorption in 39 inter-specific recombinant congenic mice strains, using in vivo ultrasound bio-microscopy. The short chromosomal intervals related to the phenotypes will facilitate the study of a restricted number of candidate genes which are potentially dysregulated in patients affected by RSA.
- Published
- 2009
- Full Text
- View/download PDF
16. DC-based vaccine loaded with acid-eluted peptides in acute myeloid leukemia: the importance of choosing the best elution method.
- Author
-
Delluc S, Tourneur L, Fradelizi D, Rubio MT, Marchiol-Fournigault C, Chiocchia G, and Buzyn A
- Subjects
- Acids chemistry, Animals, Bone Marrow immunology, Bone Marrow metabolism, Chromatography, High Pressure Liquid, Citrates chemistry, Female, Humans, Leukemia, Monocytic, Acute blood, Leukemia, Myeloid, Acute blood, Lymphocyte Activation, Mice, Mice, Inbred C57BL, Middle Aged, Peptide Fragments isolation & purification, Phosphates chemistry, T-Lymphocytes, Cytotoxic immunology, Trifluoroacetic Acid chemistry, Cancer Vaccines immunology, Dendritic Cells immunology, Leukemia, Monocytic, Acute immunology, Leukemia, Myeloid, Acute immunology, Neoplasm Proteins immunology, Peptide Fragments immunology
- Abstract
Tumor-associated peptides isolated by acid elution are frequently used for therapeutic immunization against various tumors both in mice and in humans. In acute myeloid leukemia (AML), the frequent accessibility of a large tumor burden allows for extraction of peptides from leukemia cells by using either citrate-phosphate (CP) or trifluoroacetic acid (TFA) buffer. To develop an optimal immunotherapeutic protocol for AML patients, we evaluated both in mice and in humans, the immunogenicity of peptides eluted from leukemia cells with the two acids (TFA or CP). Although ex vivo studies in mice showed that both prophylactic immunizations with mature dendritic cells (DC) loaded with TFA-peptides (DC/TFA), or CP-peptides (DC/CP), were able to stimulate specific antileukemia immune responses, only vaccination with DC/TFA was able to prevent leukemia outgrowth. Moreover, in humans, only DC/TFA generated significant antileukemia CD4(+) and cytotoxic CD8(+) T cell responses in vitro. In summary, these data demonstrate that the choice of the acid elution procedure to isolate immunogenic peptides strongly influences the efficacy of the antileukemia immune responses. These finding raise essential considerations for the development of immunotherapeutic protocols for cancer patients. In our model, our results argue for the use of the TFA elution method to extract immunogenic AML-associated peptides.
- Published
- 2007
- Full Text
- View/download PDF
17. [High-resolution ultrasound imaging of the mouse].
- Author
-
Renault G, Bonnin P, Marchiol-Fournigault C, Gregoire JM, Serrière S, Richard B, and Fradelizi D
- Subjects
- Animals, Embryo, Mammalian diagnostic imaging, Female, Forecasting, Intestinal Neoplasms diagnostic imaging, Kidney diagnostic imaging, Liver diagnostic imaging, Mice, Nude, Neoplasms diagnostic imaging, Ovary diagnostic imaging, Posture, Spleen diagnostic imaging, Ultrasonography, Doppler methods, Ultrasonography, Doppler statistics & numerical data, Uterus diagnostic imaging, Disease Models, Animal, Mice anatomy & histology, Mice embryology, Ultrasonography methods, Ultrasonography statistics & numerical data
- Abstract
Small-animal ultrasound imaging has been made possible using high-resolution imaging devices. The spatial resolution is therefore sufficient to accurately measure anatomical parameters in mice. This paper reviews some of the main applications of high-resolution ultrasound imaging of the mouse and highlights what could be the forthcoming advances.
- Published
- 2006
- Full Text
- View/download PDF
18. Inhibition of transforming growth factor-beta signaling accelerates atherosclerosis and induces an unstable plaque phenotype in mice.
- Author
-
Mallat Z, Gojova A, Marchiol-Fournigault C, Esposito B, Kamaté C, Merval R, Fradelizi D, and Tedgui A
- Subjects
- Animals, Antibodies, Monoclonal pharmacology, Aorta metabolism, Aorta pathology, Apolipoproteins E deficiency, Apolipoproteins E genetics, Arteriosclerosis pathology, Body Weight drug effects, Cholesterol, HDL blood, Collagen metabolism, Disease Progression, Immunohistochemistry, Inflammation pathology, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Phenotype, Signal Transduction drug effects, Transforming Growth Factor beta antagonists & inhibitors, Transforming Growth Factor beta1, Transforming Growth Factor beta2, Transforming Growth Factor beta3, Arteriosclerosis etiology, Arteriosclerosis metabolism, Signal Transduction physiology, Transforming Growth Factor beta metabolism
- Abstract
Atherosclerosis is a disease of the arterial wall that seems to be tightly modulated by the local inflammatory balance. Whereas a large body of evidence supports a role for proinflammatory mediators in disease progression, the understanding of the role of the antiinflammatory component in the modulation of plaque progression is only at its beginning. TGF-beta1, -beta2, and -beta3 are cytokines/growth factors with broad activities on cells and tissues in the cardiovascular system and have been proposed to play a role in the pathogenesis of atherosclerosis. However, no study has examined the direct role of TGF-beta in the development and composition of advanced atherosclerotic lesions. In the present study, we show that inhibition of TGF-beta signaling using a neutralizing anti-TGF-beta1, -beta2, and -beta3 antibody accelerates the development of atherosclerotic lesions in apoE-deficient mice. Moreover, inhibition of TGF-beta signaling favors the development of lesions with increased inflammatory component and decreased collagen content. These results identify a major protective role for TGF-beta in atherosclerosis.
- Published
- 2001
- Full Text
- View/download PDF
19. Further characterization of CD82/IA4 antigen (type III surface protein): an activation/differentiation marker of mononuclear cells.
- Author
-
Lebel-Binay S, Gil ML, Lagaudriere C, Miloux B, Marchiol-Fournigault C, Quillet-Mary A, Lopez M, Fradelizi D, and Conjeaud H
- Subjects
- Animals, Antigens, CD genetics, Antigens, CD immunology, Antigens, Surface genetics, Antigens, Surface immunology, Cell Communication, Cell Line, Chromosome Mapping, Epitopes, Humans, Immunoblotting, Kangai-1 Protein, Killer Cells, Lymphokine-Activated immunology, Lymphocyte Activation, Macrophages immunology, Mice, Mice, Inbred DBA, T-Lymphocytes immunology, Antigens, CD analysis, Antigens, Surface analysis, Leukocytes, Mononuclear immunology, Membrane Glycoproteins, Proto-Oncogene Proteins
- Abstract
The mononuclear cell surface protein IA4 was originally identified in our lab using a mAb selected because of its strong reactivity with three lymphoblastoid variant cell lines which are HLA class I deficient, are LAK susceptible, and form a high number of conjugates with LAK effectors. We previously cloned the cDNA of the IA4 protein, coding for a 267-amino-acid type III integral membrane protein, with four transmembrane domains and three possible N-glycosylation sites. The IA4 protein belongs to the tetra span transmembrane (TST) new family of surface molecules, which also includes CD9, CD37, CD53, CD63, and TAPA-1. IA4 antigen was recently recognized as belonging to a new cluster of differentiation CD82 (International CD Workshop, Boston 1993). The IA4 antigen expression pattern at the surface of immune cells from normal donors was studied. On T lymphocytes, IA4 was barely detectable on resting cells and increased 3.5- to 7-fold following PHA or PHA+PMA stimulation. This IA4 increased expression is correlated with the morphologic change in blast cells and with the expression of activation markers such as CD2 and MHC class II antigens, therefore suggesting that IA4 is an activation marker on T lymphocytes. The expression of IA4 was low on circulating resting monocytes collected by elutriation. However, these monocytes, cultured in medium alone or with GM-CSF, acquired the morphology of macrophage and simultaneously overexpressed MHC Class II, CD14, and IA4 antigens, suggesting that IA4 is a differentiation marker for macrophages, whatever the culture conditions, either adherent (plastic culture dishes) or nonadherent (Teflon culture bags). IA4 stable transfectants of the murine mastocytoma cell line P815 were obtained and used to generate a new mAb. Competitive epitope binding studies have shown that IA4 antigen presents a dominant epitope recognized by most of the mAb prepared either in our lab or elsewhere. This dominant epitope is not shared by any of the other antigens of the TST family. Using this new mAb we were able to biochemically characterize the IA4 antigen as a 28-kDa protein, highly N-glycosylated with different patterns on various cells.
- Published
- 1994
- Full Text
- View/download PDF
20. Target lysis by human LAK cells is critically dependent upon target binding properties, but LFA-1, LFA-3 and ICAM-1 are not the major adhesion ligands on targets.
- Author
-
Quillet-Mary A, Cavarec L, Kermarrec N, Marchiol-Fournigault C, Gil ML, Conjeaud H, and Fradelizi D
- Subjects
- Antibodies, Monoclonal immunology, Antigens, Differentiation, T-Lymphocyte immunology, Antigens, Differentiation, T-Lymphocyte metabolism, Antigens, Surface immunology, Burkitt Lymphoma immunology, Burkitt Lymphoma pathology, CD3 Complex, CD58 Antigens, Cell Adhesion Molecules immunology, Cell Survival physiology, Flow Cytometry methods, Fluorescence, Humans, Intercellular Adhesion Molecule-1, Killer Cells, Lymphokine-Activated immunology, Killer Cells, Lymphokine-Activated physiology, Lymphocytes immunology, Lymphocytes physiology, Lymphoma, B-Cell immunology, Lymphoma, B-Cell pathology, Membrane Glycoproteins immunology, Receptors, Antigen, T-Cell immunology, Receptors, Antigen, T-Cell metabolism, Antigens, Surface metabolism, Burkitt Lymphoma metabolism, Cell Adhesion Molecules metabolism, Killer Cells, Lymphokine-Activated metabolism, Lymphocyte Function-Associated Antigen-1 metabolism, Lymphoma, B-Cell metabolism, Membrane Glycoproteins metabolism
- Abstract
The cytotoxicity mediated by the CD2+ CD3- lymphocyte subset, either NK or LAK, is puzzling since no specific antigen recognition structures, equivalent to the CD3-associated heterodimer T-cell receptor, have been recognized on these cells so far. The possibility exists that the CD3- cytotoxic effectors recognize their targets through non-specific adhesion mechanisms. The goal of this study was: (a) to examine the correlation between binding properties and susceptibility to lysis of 6 informative target cell lines; (b) to evaluate the role, as ligands on these targets, of adhesion molecules such as LFA-1, LFA-3 and ICAM-1. The effectors used in this study were IL-2-activated LGL, predominantly CD3-, or highly purified CD3- lymphocytes from normal human donors. The 6 target lines studied included 2 pairs of EBV-transformed B-cell lines (721 LCL vs. 721.134, and MM vs. MM-10F2) in which the parental lines were resistant to lysis while HLA variants were susceptible. A third pair was the Daudi Burkitt cell line, susceptible to LAK lysis, and an HLA-positive transfected Daudi line which was more resistant to lysis. The binding properties of these targets to LAK effectors (conjugate formation) were evaluated using a sensitive double fluorescence flow cytometry method. In each pair examined, the susceptible targets formed more conjugates and were surrounded by more cytotoxic LAK effectors than their resistant counterparts, indicating that the conjugation properties of targets are closely correlated with their susceptibility to LAK lysis. The expression of adhesion molecules on the informative targets was examined by indirect immunofluorescence and their role was evaluated by inhibition of lysis after pre-coating the targets with the relevant antibodies. The differences in the expression of the classical cell-cell adhesion molecules LFA-1, LFA-3 and ICAM-1 on the target surfaces were only marginal, insufficient to explain the striking differences in susceptibility to lysis and in binding properties. Coating the target cells with antibodies directed against these adhesion determinants had no effects on the lysis of susceptible target cells. The same antibodies reacting with the LAK effectors did inhibit lysis. Taken together, these results suggest that, on the targets, presently undefined membrane adhesion structures may have a major role in conjugate formation between target and CD3- effectors and determine the susceptibility of the targets to lysis.
- Published
- 1991
- Full Text
- View/download PDF
21. An improved electrotransfection method using square shaped electric impulsions.
- Author
-
Presse F, Quillet A, Mir L, Marchiol-Fournigault C, Feunteun J, and Fradelizi D
- Subjects
- Cell Line, Cell Membrane Permeability, Cell Survival, Electric Stimulation, Humans, Isoquinolines, Lymphocytes cytology, Simian virus 40 genetics, beta 2-Microglobulin genetics, DNA analysis, Transfection methods
- Abstract
Transfection of DNA into non adherent cells can be achieved by electropermeation. Previously published results, partially successful, were obtained using exponential decaying electric impulsions. However, one limitation of this technique has been the damaging effect of this type of impulsions resulting in poor cell recovery. We report hereby the electropermeation of human lymphoblastoid cell lines using a commercially available electropulsator delivering repeated, short, high voltage, square shaped, electric pulses. The parameters of transfection have been optimized using the "Lucifer Yellow Permeation Assay". With the optimum electric parameters, virtually all the cells were permeated and at least 70% survived the shocking conditions. Both transient expression and permanent integration and expression of DNA was observed.
- Published
- 1988
- Full Text
- View/download PDF
22. Increased resistance to non-MHC-restricted cytotoxicity related to HLA A, B expression. Direct demonstration using beta 2-microglobulin-transfected Daudi cells.
- Author
-
Quillet A, Presse F, Marchiol-Fournigault C, Harel-Bellan A, Benbunan M, Ploegh H, and Fradelizi D
- Subjects
- Cytotoxicity, Immunologic, Gene Conversion, HLA Antigens immunology, HLA-A Antigens, HLA-B Antigens, Humans, Immunity, Innate, Killer Cells, Natural immunology, Tumor Cells, Cultured metabolism, beta 2-Microglobulin immunology, HLA Antigens genetics, Transfection, Tumor Cells, Cultured immunology, beta 2-Microglobulin genetics
- Abstract
Experiments in several laboratories have shown that target susceptibility to NK and lymphokine-activated killer (LAK) cytotoxicity is inversely correlated with the target expression of HLA Class I molecules. We present the first direct evidence, obtained by gene transfection, that target cell HLA, A, B expression increases the resistance to the "so-called" non-MHC-restricted cytotoxicity. We have co-transfected, by electroporation, the human beta 2-microglobulin gene and the gene carrying the resistance to geneticin into Daudi cell line. Geneticin selection in culture followed by FACS sorting on the basis of strong positivity with the mAb W6/32 (which is specific for the HLA class I H chain associated to beta 2-microglobulin) have led to the establishment of a HLA+ Daudi cell line permanently expressing HLA A10, A11, and B17 molecules. Studies were performed in vitro to evaluate the susceptibility of these cells to either NK and LAK cytotoxicity. The HLA class I+ Daudi cells exhibit an increased resistance to killing by non-MHC-restricted killer cells (both NK and LAK) as compared with their HLA-Daudi counterpart.
- Published
- 1988
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.