Search

We performed an integrated phylogeographical and palaeoclimatic study of an early-diverging member of Asteraceae, Duseniella patagonica, endemic to Argentina. Chloroplast and nuclear markers were sequenced from 106 individuals belonging to 20 populations throughout the species range. We analysed genetic spatial distribution, diversity and structure, tested for range expansion, estimated divergence times, reconstructed ancestral areas and modelled present and past species distributions based on climatic data. Duseniella diverged from its sister genera during the Late/Middle Miocene. Its ancestral area included southern Monte plus eastern and central Patagonia. A vicariant event separated Monte and Patagonian clades during the Plio-Pleistocene. This would have involved unfavourable climate, soil, elevation, volcanism and/or other geomorphological processes between 40 and 43.5°S, in the sourroundings of the Somuncura plateau. Each clade possesses its own haplotypes and nucleotypes. Two populations, one in southern Monte and the other in eastern Patagonia, contain the highest diversity and exclusive haplotypes, representing hypothetical ancestral refugia. Northern Monte and southern Patagonian populations show low to null genetic diversity, being the most recently colonized areas. Climatic models indicate that winter temperature influenced the distribution of Duseniella, with an increase in probability of occurrence during colder periods, thus enabling diversification during glacial episodes.

Congruence among different sources of data is highly desirable in phylogenetic analyses. However, plastid and nuclear DNA may record different evolutionary processes such that incongruence among results from these sources can help unravel complex evolutionary histories. That is the case of Nassauvia subgenus Strongyloma (Asteraceae), a taxon with five putative species distributed in the southern Andes and Patagonian steppe. Morphometric and phylogeographic information cast doubt on the integrity of its species, and previous molecular data even questioned the monophyly of the subgenus. We tested those questions using plastid and nuclear DNA sequences by the application of different methods such as phylogenetic trees, networks, a test of genealogical sorting, an analysis of population structure, calibration of the trees, and hybridization test, assembling non-synchronous incongruent results at subgenus and species levels in a single reconstruction. The integration of our molecular analyses and previous taxonomic, morphological, and molecular studies support subgenus Strongyloma as a monophyletic group. However, the topology of the nuclear trees and the evidence of polyploids within subgenus Nassauvia, suggest a hypothetical origin and initial radiation of Nassauvia related to an ancient hybridization event that occurred around 17–6.3 Myr ago near the Andes in west-central Patagonia. Plastid data suggest a recent diversification within subgenus Strongyloma, at most 9.8 Myr ago, towards the Patagonian steppe east of the Andes. These processes cause phylogenies to deviate from the species tree since each putative species lack exclusive ancestry. The non-monophyly of its species using both plastid and nuclear data is caused mainly by incomplete lineage sorting occurred since the Miocene. The final uplift of the Andes and PliocenePleistocene glacial-interglacial and its consequences on the landscape and climate structured the genetic composition of this group of plants in the Patagonian steppe. The molecular data presented here agree with previous morphological studies, in that the five putative species typically accepted in this subgenus are not independent taxa. This study emphasizes that adding more than one sequence per species, not combining data with dissimilar inheritance patterns without first performed incongruence tests, exploring data through different methodologies, considering the timing of events, and searching for the causes of poorly resolved and/or incongruent phylogenies help to reveal complex biological underlying processes, which might otherwise remain hidden. Fil: Nicola, Marcela Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; Argentina Fil: Johnson, Leigh. University Brigham Young; Estados Unidos Fil: Pozner, Raúl Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; Argentina

We describe and illustrate Mastigostyla coronata, a new species of Iridaceae from the high Andes of Jujuy (Argentina), morphologically similar to Mastigostyla vargasii. The inclusion of this new species in Mastigostyla is supported by a phylogenetic analysis based on plastid markers. We also present a morphological comparison among the sub-acaulescent species of Mastigostyla (including Cardenanthus), and a key to those species.

We asked young scientists this question: How do broad interests benefit your science? Scientists with a variety of hobbies responded that their extracurricular activities have enhanced a wide range of skills, from creativity to communication to resilience. Many also mentioned the value of clearing their minds and relaxing. Follow NextGen and share your own hobbies on Twitter with #NextGenSci. As a rock climber, you have to risk falling in order to become better ; the same principle applies in science. Realizing this has made me look at research failures in a more positive light—less as a demonstration of incompetence, and more of an opportunity to learn something and build resilience. I've learned that reminding yourself of your own competence (on the wall or in the lab) is a useful way to build up the courage to take more risks.

The new genus Kellochloa is proposed, on the basis of morphological and molecular characters, to include two North American species of Panicum s.l., previously classified in sect. Verrucosa: P.brachyanthum and P. verrucosum. Both species are annual, with decumbent culms branching divaricately at the lower nodes, and erect culms with terminal and axillary inflorescences. The spikelets are pilose, with lower glume 1/6 to 1/4 the length of the spikelet, upper glume and lower lemma subequal, 3–5-nerved, the lower anthecium reduced to the lower lemma, and upper anthecium indurate, with bicellular microhairs and simple papillae evenly distributed all over the lemma and palea. Molecular phylogenetic studies, analyzing plastid ndhF and rpoA sequences, confirmed that these morphological characters are differential. The phylogenetic position and taxonomic features of the new genus are established and compared with Panicum s.str. and other taxa of the Sacciolepis–Trichanthecium clade. Full synonymies and descriptions, a distribution map, illustrations of both species, and a key are provided. Two new combinations are proposed. In addition, four African ungrouped species of Panicum s.l. were, for the first time, included in the phylogenetic analysis to analyze their relationship with the new genus and its positions in the Sacciolepis–Trichanthecium clade. Fil: Nicola, Marcela Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; Argentina Fil: Lizarazu, Mabel Angela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; Argentina Fil: Scataglini, María Amalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; Argentina

During the revision of Siphocampylus for the Flora of Argentina, we detected names which need lectotypification. As a result of our analysis of original materials and protologues, lectotypes are designated for the following names: S. fiebrigii var. intermedius, S. foliosus, S. foliosus var. minor, S. lorentzii, S. nemoralis, S. nemoralis var. grisebachii, S. nemoralis fo. hieronymi, S. orbignianus, and S. verticillatus. Fil: Salomón, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; Argentina Fil: Nicola, Marcela Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; Argentina Fil: Nomdedeu, Soledad María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; Argentina

The Patagonian steppe is an immense, cold, arid region, yet phylogeographically understudied. Nassauvia subgen. Strongyloma is a characteristic element of the steppe, exhibiting a continuum of morphological variation. This taxon provides a relevant phylogeographical model not only to understand how past environmental changes shaped the genetic structure of its populations, but also to explore phylogeographical scenarios at the large geographical scale of the Patagonian steppe. Here, we (1) assess demographic processes and historical events that shaped current geographic patterns of haplotypic diversity; (2) analyze hypotheses of isolation in refugia, fragmentation of populations, and/or colonization of available areas during Pleistocene glaciations; and (3) model extant and palaeoclimatic distributions to support inferred phylogeographical patterns. Chloroplast intergenic spacers, rpl32–trnL and trnQ–5′rps16, were sequenced for 372 individuals from 63 populations. Nested clade analysis, analyses of molecular variance, and neutrality tests were performed to assess genetic structure and range expansion. The present potential distribution was modelled and projected onto a last glacial maximum (LGM) model. Of 41 haplotypes observed, ten were shared among populations associated with different morphological variants. Populations with highest haplotype diversity and private haplotypes were found in central-western and south-eastern Patagonia, consistent with long-term persistence in refugia during Pleistocene. Palaeomodelling suggested a shift toward the palaeoseashore during LGM; new available areas over the exposed Atlantic submarine platform were colonized during glaciations with postglacial retraction of populations. A scenario of fragmentation and posterior range expansion may explain the observed patterns in the center of the steppe, which is supported by palaeomodelling. Northern Patagonian populations were isolated from southern populations by the Chubut and the Deseado river basins during glaciations. Pleistocene glaciations indirectly impacted the distribution, demography, and diversification of subgen. Strongyloma through decreased winter temperatures and water availability in different areas of its range. Fil: Nicola, Marcela Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; Argentina Fil: Nicola, Marcela Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; Argentina Fil: Pozner, Raul Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; Argentina Fil: Johnson, Leigh. University Brigham Young; Estados Unidos

Species of Paspalum group Gardneriana are revised as a whole for the first time. Seven species are treated in this work, being the group characterized by including annual or perennial species, with spikelets usually obovoid, upper glume absent or reduced, and upper anthecium indurate and strongly papillose, with verrucose papillae all over its surface. A key to the species is provided, together with a discussion of previous infrageneric classifications and relationships of Gardneriana; morphological descriptions, and illustrations of all species are also presented. The following species are included in Paspalum group Gardneriana: Paspalum burchellii, P. delicatum, P. gardnerianum, P. nudatum, P. pictum, P. reduncum, and P. subsesquiglume. Also, lectotypes are designated for P. burchellii, P. connectens, P. reduncum, and P. subsesquiglume; P. gemmosum is defined as a new synonym of P. subsesquiglume.

Morphological variation among the five species of Nassauvia subgenus Strongyloma was assessed through statistical analyses of morphometric traits in populations throughout the southern Andean-Patagonian region. Uni- and multivariate analyses were used to identify patterns of morphological variation in relation to geography. Additionally, species distribution modeling was implemented to relate these patterns to climatic conditions. No well-defined groups could be recovered through multivariate analyses, although we observed some geographic structure. Latitudinal variation was found in leaves, phyllaries and cypselas, with a cline towards the south, where these structures become shorter and wider. Towards the east, the number of flowers per capitulum decreases, and abaxial corolla lips and cypselas become narrower and shorter. Distribution modeling showed several areas of contact and a large overlap of suitable conditions for more than one species, which is mainly related to the mean temperature of winter. Despite an association between morphological variation with geography and climate, actual geographic distributions of the putative species did not entirely match the clinal pattern of morphology, geography, and climate. Further genetic analyses are still needed to identify the probable processes that led to the complex patterns of observed variation.

Aim An integrative study of the endemic, yet ubiquitous, Patagonian shrub Mulinum spinosum (Apiaceae) was performed: (1) to assess the historical processes that influenced its geographical pattern of genetic variation; (2) to test hypotheses of its survival in situ or in glacial refugia during glacial cycles; and (3) to model its extant and palaeoclimatic distributions to assess support for the phylogeographical patterns recovered. Location Chilean and Argentinian Andean region and Patagonian steppe. Methods Chloroplast DNA sequences, trnH–psbA, trnS–trnG and 3′trnV–ndhC, were obtained for 314 individuals of M. spinosum from 71 populations. The haplotype data matrix was analysed using nested clade analysis (NCA) to construct a network. Analysis of molecular variance (AMOVA), spatial analysis of molecular variance (SAMOVA) and neutrality tests were also used to test for genetic structure and range expansion in the species. The present potential geographical distribution of M. spinosum was modelled and projected onto a Last Glacial Maximum (LGM) model. Results Amongst the 29 haplotypes observed, one was widely distributed, but most were restricted to either northern or southern regions. The populations with highest haplotype diversity were found in southern Patagonia, the high Andean region, and northern Patagonia. AMOVA and SAMOVA showed latitudinal structure for Argentinian populations. NCA implied patterns of restricted gene flow or dispersal but with some long-distance dispersal and also long-distance colonization and/or past fragmentation. Neutrality tests did not support range expansions. The current distribution model was a fairly good representation of the extant geographical distribution of the species, and the distribution model for the LGM did not show important shifts of the extant range to lower latitudes, except for a shift towards the palaeoseashore. Main conclusions Based on agreement amongst phylogeographical patterns, distribution of genetic variability, equivocal evidence of putative refugia and palaeodistribution modelling, it is probable that glaciations did not greatly affect the distribution of Mulinum spinosum. Our results are consistent with the in situ survival hypothesis, and not with the latitudinal migration of plant communities to avoid adverse climate conditions during Pleistocene glaciations. It is possible that populations of northern Patagonia may have been isolated from the southern ones by the Chubut and Deseado basins.

We describe a new species of Sicydium endemic to Argentina. Relationships and differences with morphologically and geographically closest species are discussed, including a key for material with staminate flowers to all species of Sicydium, and an illustration of the new species. Fil: Nicola, Marcela Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; Argentina Fil: Donadío, Sabina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; Argentina Fil: Pozner, Raul Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; Argentina

A new apetalous species of Minuartia from the high Andes of northwestern Argentina, M. altoandina, is here described and illustrated. At first sight, M. altoandina is morphologically very similar to the European M. sedoides because of the absence of petals and ciliate leaf margin with narrow hyaline teeth; however, from a biogeographical point of view, it will probably be related to the North American M. rossii complex through the morphology of M. austromontana.