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Salt marshes are crucial eco-geomorphic features of tidal environments as they provide important ecological functions and deliver a wide range of ecosystem services. Being controlled by the interplay between hydrodynamics, geomorphology, and vegetation, the contribution of both organic matter (OM) and inorganic sediments drives salt marsh vertical accretion. This allows marshes to keep pace with relative sea level rise and likewise capture and store carbon, making them valuable allies in climate mitigation strategies. Thus, soil organic matter (SOM), i.e. the organic component of the soil, plays a key role within salt marsh environments, directly contributing to soil formation and supporting carbon storage. This study aims at inspecting spatial patterns of OM in surface salt marsh soils (top 20 cm), providing further insights into the physical and biological factors driving OM dynamics that affect salt marsh survival and carbon sink potential. Our results reveal two scales of variations in SOM content in marsh environments. At the marsh scale, OM variability is influenced by the interplay between surface elevation and changes in sediment supply linked with the distance from the marsh edge. At the system scale, OM content distribution is dominated by the gradient generated by marine and fluvial influence. The observed variations in SOM are explained by the combination of inorganic and organic input, preservation conditions, and sediment grain size. Our results highlight the importance of marshes as carbon sink environments, further emphasising that environmental conditions within a tidal system may generate strongly variable and site-specific carbon accumulation patterns, enhancing blue carbon assessment complexity.

Abstract Hidradenitis suppurativa (HS) is a debilitating chronic skin disorder characterized by painful inflammatory nodules, abscesses and sinus tracts involving intertriginous areas and has an adverse impact on patient quality of life. Over the past decade, the therapeutic options of HS have increased significantly to comprise multiple modalities, including topical medication, systemic therapies (mainly antibiotics, retinoids, and biologics), surgical approaches, and lifestyle modifications. Biologics alone or in combination with surgery remain the treatment of choice for moderate to severe disease. However, non-biologic therapies (including retinoids) may be used as monotherapy for mild disease and in combination with biologics and surgical treatment in moderate to severe disease. Retinoids, specifically isotretinoin, acitretin, and alitretinoin, are historically used in the management of HS, supported by anecdotal evidence and with variable treatment response. Although the current American and European guidelines offer different recommendations on the use of retinoids in HS, retinoids remain a valuable ally in HS management. This review provides a comprehensive analysis of the current scientific literature on retinoid therapy (topical and systemic) in HS, highlighting disparities in mechanisms of action, efficacy, and safety to clarify their role in HS treatment.

The effect of coating a flat blade surface with titanium nitride nano coatings (TiN), nano tantalum carbide (TaC), Fiberglass (Glass Fiber-Reinforced Polymer) (GFRP), Galvanized Steel (GAS), and St37 (SST37) was investigated in order to decrease the adhesion of soil on tilling tools, external friction and, ultimately, the draft force. The soil tank, which was filled with soil of the desired conditions, was pulled on the bearing on the rail. A S-shaped load cell was used to measure the draft force. Tests were conducted at a distance of 2 m and speeds of 0.1, 0.2, and 0.3 m·s−1 at a depth of 10 cm. A model based on input factors, including blade travel speed, rake angle, and cohesion and adhesion of soil–blade, was developed in an adaptive neuro-fuzzy inference system (ANFIS), and draft force was the output parameter. To verify the performance of the developed model using ANFIS, a relative error(ε) of 6.1% and coefficient of determination (R2) of 0.956 were computed. It was found that blades coated with Nano (TiN-TaC), due to its hydrophobic surface, flatness, and self-cleaning properties, have considerable ability to decrease adhesion in wet soils and showed a linear relationship with draft force reduction.

The Magic project (MArine Geohazard along Italian Coasts), a large coordinated initiative that involved the whole marine geological research community in Italy in 2007–2013, produced a series of maps of the Geohazard features of the Central-Southern Tyrrhenian Seamounts. The features derive from multibeam surveys and therefore mainly rely on the morphological expression of seafloor and shallow sub-surface processes and events. Potential geohazards related to eruption and flank instability of the volcanic seamounts of the Tyrrhenian Sea are discussed and compared.

The MaGIC project (Marine Geohazard along the Italian Coasts) had the aim of mapping the geohazard in the Italian seas and resulted in numerous maps. In this paper, we present the maps: ‘Maps of Geohazard features of the eastern Sardinian Margin’. The Eastern Sardinia margin has mainly a narrow shelf, which reaches a width of 20 km only to the north. At the base-of-slope, a series of intra-slope basins sits in the structural depressions formed during the opening of the Tyrrhenian back-arc system. Submerged, coastal depositional bodies are present in the Olbia area where the continental shelf is large. They most likely represent the remnants of coastal environments formed during the last rise in sea level. They can represent important geo-resources for application in the issue of coastal erosion management. They are also the site of ecosystems hosting a specific biodiversity, which need to be preserved. In the central and southern part of the mapped area, the shelf is very narrow with an average width of about 4 km. Here, many canyon heads are very close to the coastline. They develop through retrogadational processes. Particularly in those canyons, where the canyon heads reach the coast, sediment instability represents an important source of geohazards to the coastal regions.

ABSTRACTThe MaGIC project (Marine Geohazard along the Italian Coasts) had the aim of mapping the geohazard in the Italian seas and resulted in the production of numerous maps covering parts of the Italian Seas. In this paper, we present the maps: ‘The submerged portions of the Aeolian volcanic islands and the north-eastern Sicilian margin’, located in the south-eastern Tyrrhenian Sea. Both areas are affected by active geological processes, which represent important geohazards elements. Inthe submarine parts of the Stromboli volcanoremobilization of volcaniclastic deposits occur along the Sciara del Fuoco, where small-scale instabilities may represent a source of geohazard. Hydrothermal activity occurs on Enarete and Enaretino conical seamounts. The north-eastern Sicilian margin has a narrow continental shelf. Numerous canyon heads indent the shelf and, sometimes, reach close to the coast. Canyons have often a retrogradational trend and further eventual landward shift through sliding can iendangeri coastal or offshore infrastructures. Many of the canyons connect with leveed channels with widespread sediment instability. In the Gioia Basin, some of the channels connect to form the Stromboli slope Valley. Volcanic unrest or local and regional earthquakes are proven to have caused submarine landslides and tsunamis.

Summary: Despite extensive global research into genetic predisposition for severe COVID-19, knowledge on the role of rare host genetic variants and their relation to other risk factors remains limited. Here, 52 genes with prior etiological evidence were sequenced in 1,772 severe COVID-19 cases and 5,347 population-based controls from Spain/Italy. Rare deleterious TLR7 variants were present in 2.4% of young (

Buscamos reconhecer como conhecimentos feministas contribuem para (re)interpretações teórico-metodológicas, a partir da produção de pesquisas/intervenções relacionadas à gênero no contexto da cultura física. Alicerçados numa abordagem qualitativa, selecionamos textos feministas dos Estudos Culturais Físicos. Como resultado, argumentamos que os textos exploram formas de teorizar gênero e as múltiplas operações do poder social a partir da radicalidade do corpo como local central dessas relações; apontam para a autorreflexidade exercida pelas pesquisadoras ao narrarem suas experiências em pesquisas; e, acenam para o imperativo político como base para a mudança social progressiva, engajando-se com questões que transcendem a categoria “gênero”.

Seagrass meadows are a highly productive and economically important shallow coastal habitat. Their sensitivity to natural and anthropogenic disturbances, combined with their importance for local biodiversity, carbon stocks, and sediment dynamics, motivate a frequent monitoring of their distribution. However, generating time series of seagrass cover from field observations is costly, and mapping methods based on remote sensing require restrictive conditions on seabed visibility, limiting the frequency of observations. In this contribution, we examine the effect of accounting for environmental factors, such as the bathymetry and median grain size (D50) of the substrate as well as the coordinates of known seagrass patches, on the performance of a random forest (RF) classifier used to determine seagrass cover. Using 148 Landsat images of the Venice Lagoon (Italy) between 1999 and 2020, we trained an RF classifier with only spectral features from Landsat images and seagrass surveys from 2002 and 2017. Then, by adding the features above and applying a time-based correction to predictions, we created multiple RF models with different feature combinations. We tested the quality of the resulting seagrass cover predictions from each model against field surveys, showing that bathymetry, D50, and coordinates of known patches exert an influence that is dependent on the training Landsat image and seagrass survey chosen. In models trained on a survey from 2017, where using only spectral features causes predictions to overestimate seagrass surface area, no significant change in model performance was observed. Conversely, in models trained on a survey from 2002, the addition of the out-of-image features and particularly coordinates of known vegetated patches greatly improves the predictive capacity of the model, while still allowing the detection of seagrass beds absent in the reference field survey. Applying a time-based correction eliminates small temporal variations in predictions, improving predictions that performed well before correction. We conclude that accounting for the coordinates of known seagrass patches, together with applying a time-based correction, has the most potential to produce reliable frequent predictions of seagrass cover. While this case study alone is insufficient to explain how geographic location information influences the classification process, we suggest that it is linked to the inherent spatial auto-correlation of seagrass meadow distribution. In the interest of improving remote-sensing classification and particularly to develop our capacity to map vegetation across time, we identify this phenomenon as warranting further research.

In this letter we present a current mode gate based on differential pair as an application of carbon nanotube field effect transistors (CNTFETs). The proposed circuit has two output logic gates: one is NAND, and the other is AND. To simplify the circuit realization we use all CNTFETs of the same type, all with the same lengths and carbon nanotube symmetry indices (n, m). Complex circuits could be obtained in current mode replicating the differential pair CNTFET along the current path. The proposed procedure allows simulation of transfer characteristics from voltage input to current output but also from voltage input to voltage output. Moreover, we can measure simulated power dissipation and delay times.

BackgroundThis study provides a global overview of the management of patients with acute cholecystitis during the initial phase of the COVID-19 pandemic.MethodsCHOLECOVID is an international, multicentre, observational comparative study of patients admitted to hospital with acute cholecystitis during the COVID-19 pandemic. Data on management were collected for a 2-month study interval coincident with the WHO declaration of the SARS-CoV-2 pandemic and compared with an equivalent pre-pandemic time interval. Mediation analysis examined the influence of SARS-COV-2 infection on 30-day mortality.ResultsThis study collected data on 9783 patients with acute cholecystitis admitted to 247 hospitals across the world. The pandemic was associated with reduced availability of surgical workforce and operating facilities globally, a significant shift to worse severity of disease, and increased use of conservative management. There was a reduction (both absolute and proportionate) in the number of patients undergoing cholecystectomy from 3095 patients (56.2 per cent) pre-pandemic to 1998 patients (46.2 per cent) during the pandemic but there was no difference in 30-day all-cause mortality after cholecystectomy comparing the pre-pandemic interval with the pandemic (13 patients (0.4 per cent) pre-pandemic to 13 patients (0.6 per cent) pandemic; P = 0.355). In mediation analysis, an admission with acute cholecystitis during the pandemic was associated with a non-significant increased risk of death (OR 1.29, 95 per cent c.i. 0.93 to 1.79, P = 0.121).ConclusionCHOLECOVID provides a unique overview of the treatment of patients with cholecystitis across the globe during the first months of the SARS-CoV-2 pandemic. The study highlights the need for system resilience in retention of elective surgical activity. Cholecystectomy was associated with a low risk of mortality and deferral of treatment results in an increase in avoidable morbidity that represents the non-COVID cost of this pandemic.

Dalam upaya tata laksana fibriasi atrium (FA) untuk mencegah risiko stroke, antikoagulan seperti warfarin dapat digunakan. Indeks terapi yang sempit mendukung bahwa pengawasan selama penggunaan warfarin diperlukan untuk mencegah risiko perdarahan. Penelitian ini bertujuan untuk menggambarkan status risiko perdarahan pada sekelompok pasien FA yang menggunakan warfarin berdasarkan nilai HAS-BLED di RS ABC Gianyar, Bali. Penelitian observasional deskriptif dilakukan dengan pendekatan retrospective case study terhadap data pasien rawat jalan di RS ABC Gianyar. Penelitian ini mengikutsertakan seluruh pasien FA penerima warfarin berdasarkan riwayat selama periode Januari 2022-Mei 2023. Pencatatan dan asesmen risiko perdarahan mencakup komponen penilaian HAS-BLED yang diilustrasikan dalam bentuk tabel dan gambar sesuai profil pasien keseluruhan. Dari sejumlah delapan pasien FA, lima pasien (62,5%) memiliki risiko perdarahan sedang (nilai 1-2), dan sisanya berada dalam kategori risiko rendah (nilai 0). Mayoritas proporsi pada stratifikasi risiko perdarahan sedang dipengaruhi oleh parameter usia lanjut >65 tahun (50,00%), penggunaan antiplatelet atau antiinflamasi nonsteroid (NSAID) (25,00%), dan nilai INR yang tinggi (12,50%). Meskipun tidak ditemukan risiko perdarahan tinggi, pemantauan selama penggunaan warfarin diperlukan dengan berfokus pada faktor risiko yang dapat dimodifikasi. Keterbatasan jumlah sampel dan beberapa parameter laboratorium pada penelitian ini dapat menjadi masukan untuk kemajuan praktik klinis maupun pengembangan penelitian selanjutnya

Summary: Background: Pre-exposure vaccination with MVA-BN has been widely used against mpox to contain the 2022 outbreak. Many countries have defined prioritized strategies, administering a single dose to those historically vaccinated for smallpox, to achieve quickly adequate coverage in front of low supplies. Using epidemiological models, real-life effectiveness was estimated at approximately 36%–86%, but no clinical trials were performed. Few data on MVA-BN immunogenicity are currently available, and there are no established correlates of protection. Immunological response in PLWH in the context of the 2022 outbreak was also poorly described. Methods: Blood samples were collected from participants eligible for pre-exposure MVA-BN vaccination before (T1) receiving a full course of vaccine (single-dose for vaccine-experienced or smallpox-primed and two-dose for smallpox vaccine-naïve or smallpox non-primed) and one month after the last dose (T2 and T3, respectively). MPXV-specific IgGs were measured by in-house immunofluorescence assay, using 1:20 as screening dilution, MPXV-specific nAbs by 50% plaque reduction neutralization test (PRNT50, starting dilution 1:10), and IFN-γ-producing specific T cells to MVA-BN vaccine, by ELISpot assay. Paired or unpaired t-test and Wilcoxon or Mann–Whitney test were used to analyse IgG and nAbs, and T-cell response, as appropriate. The probability of IgG and nAb response in vaccine-experienced vs. vaccine-naïve was estimated in participants not reactive at T1. The McNemar test was used to evaluate vaccination's effect on humoral response both overall and by smallpox vaccination history. In participants who were not reactive at T1, the proportion of becoming responders one month after full-cycle completion by exposure groups was compared by logistic regression and then analysed by HIV status strata (interaction test). The response was also examined in continuous, and the Average Treatment Effect (ATE) of the difference from baseline to schedule completion according to previous smallpox vaccination was estimated after weighting for HIV using a linear regression model. Self-reports of adverse effects following immunization (AEFIs) were prospectively collected after the first MVA-BN dose (T1). Systemic (S-AEFIs: fatigue, myalgia, headache, GI effects, chills) and local (L-AEFIs: redness, swelling, pain) AEFIs were graded as absent (grade 0), mild (1), moderate (2), or severe (3). The maximum level of severity for S-AEFIs and L-AEFIs ever experienced over the 30 days post-dose by vaccination exposure groups were analysed using a univariable multinomial logistic regression model and after adjusting for HIV status; for each of the symptoms, we also compared the mean duration by exposure group using an unpaired t-test. Findings: Among the 164 participants included, 90 (54.8%) were smallpox vaccine-experienced. Median age was 49 years (IQR 41–55). Among the 76 (46%) PLWH, 76% had a CD4 count >500 cells/μL. There was evidence that both the IgG and nAbs titers increased after administration of the MVA-BN vaccine. However, there was no evidence for a difference in the potential mean change in humoral response from baseline to the completion of a full cycle when comparing primed vs. non-primed participants. Similarly, there was no evidence for a difference in the seroconversion rate after full cycle vaccination in the subset of participants not reactive for nAbs at T1 (p = 1.00 by Fisher's exact test). In this same analysis and for the nAbs outcome, there was some evidence of negative effect modification by HIV (interaction p-value = 0.17) as primed people living with HIV (PLWH) showed a lower probability of seroconversion vs. non-primed, and the opposite was seen in PLWoH. When evaluating the response in continuous, we observed an increase in T-cell response after MVA-BN vaccination in both primed and non-primed. There was evidence for a larger increase when using the 2-dose vs. one-dose strategy with a mean difference of −2.01 log2 (p ≤ 0.0001), after controlling for HIV. No evidence for a difference in the risk of developing any AEFIs of any grade were observed by exposure group, except for the lower risk of grade 2 (moderate) fatigue, induration and local pain which was lower in primed vs. non-primed [OR 0.26 (0.08–0.92), p = 0.037; OR 0.30 (0.10–0.88), p = 0.029 and OR 0.19 (0.05–0.73), p = 0.015, respectively]. No evidence for a difference in symptom duration was also detected between the groups. Interpretation: The evaluation of the humoral and cellular response one month after the completion of the vaccination cycle suggested that MVA-BN is immunogenic and that the administration of a two-dose schedule is preferable regardless of the previous smallpox vaccination history, especially in PLWH, to maximize nAbs response. MVA-BN was safe as well tolerated, with grade 2 reactogenicity higher after the first administration in vaccine-naïve than in vaccine-experienced individuals, but with no evidence for a difference in the duration of these adverse effects. Further studies are needed to evaluate the long-term duration of immunity and to establish specific correlates of protection. Funding: The study was supported by the National Institute for Infectious Disease Lazzaro Spallanzani IRCCS “Advanced grant 5 × 1000, 2021” and by the Italian Ministry of Health “Ricerca Corrente Linea 2”.

The discovery of the interactome of cannabidiol (CBD), a non-psychoactive cannabinoid from Cannabis sativa L., has been here performed on chronic myelogenous leukemia cancer cells, using an optimized chemo-proteomic stage, which links Drug Affinity Responsive Target Stability with Limited Proteolysis Multiple Reaction Monitoring approaches. The obtained results showed the ability of CBD to target simultaneously some potential protein partners, corroborating its well-known poly-pharmacology activity. In human chronic myelogenous leukemia K562 cancer cells, the most fascinating protein partner was identified as the 116 kDa U5 small nuclear ribonucleoprotein element called EFTUD2, which fits with the spliceosome complex. The binding mode of this oncogenic protein with CBD was clarified using mass spectrometry-based and in silico analysis.

Klebsiella pneumoniae strains that are resistant to multiple drugs (KPMDRs), which are often acquired in hospital settings and lead to healthcare-associated infections, pose a serious public health threat, as does hypervirulent K. pneumoniae (hvKp), which can also cause serious infections in otherwise healthy individuals. The widespread and often unnecessary use of antibiotics seen during the recent COVID-19 pandemic has exacerbated the challenges posed by antibiotic resistance in clinical settings. There is growing concern that hypervirulent (hvKp) strains may acquire genes that confer antimicrobial resistance, thus combining an MDR profile with their increased ability to spread to multiple body sites, causing difficult-to-treat infections. This study aimed to compare resistance and virulence profiles in KPC-3-producing K. pneumoniae isolates collected over four years (2020–2023). A genome-based surveillance of all MDR CRE-K. pneumoniae was used to identify genetic differences and to characterize the virulence and resistance profiles. Our results provide a picture of the evolution of resistance and virulence genes and contribute to avoiding the possible spread of isolates with characteristics of multi-drug resistance and increased virulence, which are thought to be one of the main global challenges to public health, within our hospital.

Tobacco use is one of the main risk factors for non-communicable diseases. Avoiding youth initiation and treating addiction are fundamental public health issues to ensure better health. Among tobacco control policies, increasing tobacco price is the single most effective intervention. It reduces tobacco consumption, especially among youths, while representing a government financing source. This study aimed to assess the agreement with the proposal of a one-euro increase in tobacco price earmarked to health issues among students at Sapienza University. Two convenience samples were surveyed, five years apart, on World No Tobacco Days. Smoking habits, agreement with the proposal and reasons for it were collected. Results from the 208 questionnaires (107 in 2014, 101 in 2019) showed 46.6% of agreement with the proposal (53.3% in 2014, 39.2% in 2019, p = 0.044). Main predictive factor for agreement was being a non-smoker (OR = 6.33 p < 0.001), main reason (64.8%) was it could trigger smokers to quit or reduce consumption. Several factors might have influenced this finding, including the introduction of novel tobacco products and their increased advertisement on social media. In 2024, European Union is planning to update the Tobacco Taxation Directive which could greatly contribute to the reduction of non-communicable diseases and premature deaths.

This study aims to determine the effect of learning models and IQ on knowledge of a healthy balanced nutritious diet. Used an experimental research method with a treatment design by level 2x2. The results of study (1) there is a difference in the increase in knowledge of students who are given a jigsaw type cooperative learning model and guided inquiry learning model based on the value of F(OA) = 50.109 and p-value = 0,000 < 0,05. (2) There is an interaction between the learning model (factor A) and IQ (factor B) towards increasing knowledge of a healthy, nutritious and balanced diet based on the value of F(OAB) = 18.12 and p-value = 0,000 < 0,05. (3) there are differences in the knowledge of students who have a high IQ with a balanced, healthy, nutritious diet using the jigsaw cooperative learning model and the guided inquiry learning model. Based on Qhitung = 3,10 > Qtabel (0,05;4:30) = 2,89. (4) there is no difference in the knowledge of students who have a low IQ with the cooperative learning type of jigsaw and guided inquiry learning models based on the value of Qcount = 0.47 < Qtabel (0,05;4:30) = 2,89

As valuable and vulnerable blue carbon ecosystems, salt marshes require adaptable and robust monitoring methods that span a range of spatiotemporal scales. The application of unmanned aerial vehicle (UAV) based remote sensing is a key tool in achieving this goal. Due to the particular characteristics of tidal wetlands, however, there are challenges in obtaining research and management relevant data with the requisite level of accuracy. In this study, the spatial patterns in uncertainty stemming from scan angle, binning method, vegetation structure and platform surface morphology are examined in the context of UAV light detection and ranging (LiDAR) derived digital elevation models (DEM). The results demonstrate that overlapping the UAV flight paths sufficiently to avoid sole reliance on LIDAR data with scan angles exceeding 15 degrees is advisable. Furthermore, the spatial arrangement of halophyte species and marsh morphology has a clear influence on DEM accuracy. The largest errors were associated with sudden structural transitions at the marsh channel boundaries. The DEMmean was found to be the most accurate for bare ground, while the DEMmin was the most accurate for channels and the middle to high marsh vegetation (MAEs = −0.01m). For the low to middle vegetation, all the trialled DEMs returned a similar magnitude of mean error (MAE = ± 0.03m). The accuracy difference between the two vegetation associations examined appears to be connected to variations in coverage, height and biomass. Overall, these findings reinforce the link between salt marsh biogeomorphic complexity and the spatial distribution and magnitude of LiDAR DEM error.

ABSTRACTIn this article, the time to calculate the optimal height of the UAV is investigated as an important factor in determining the total flight time, energy consumption and total delay. In particular, in this paper, the calculation of the optimal height of the UAV, reducing the time of calculating the optimal height, reducing the energy consumption, reducing the total flight time and reducing the total delay is done. First, using the average path loss and UAV transmitted power functions, we present the optimal height of the UAV in the form of an optimization problem with a convex altitude range. Then, using the golden section search (GSS) algorithm and based on the condition of the function being unimodal, we calculate the optimization problem and obtain the optimal height value, which is the minimum of the average functions of the path loss and the transmitted power of the UAV. Also, using the convexity principle, we show that the average path loss function is convex in the mentioned interval. Next, using the relationship between the time to calculate the optimal height of the drone and the total flight time, we calculate the amount of energy consumed and the total delay. The simulation results using MATLAB show that the time to calculate the optimal height with the proposed algorithm is much faster than other methods. The time to calculate the optimal height in the proposed method is 0.03 s. The energy consumption using the proposed method is 53 kJ, and the flight time is 37 s, considering the stop on the way, which is the lowest value compared to other methods. Also, the total delay in the proposed method is less than in other methods.

Este artigo tem como objetivo principal compreender a trajetória de construção dos Estudos Culturais Físicos (ECF) antes de sua institucionalização no meio acadêmico. A problemática central reside na escassez de informações disponíveis sobre os Estudos Culturais Físicos (ECF) em seu estágio inicial, antes de serem formalmente reconhecidos no contexto acadêmico. A metodologia adotada neste estudo consistiu em uma abordagem de pesquisa bibliográfica, conduzida por meio de leituras exploratórias. A partir dessas análises, considera-se que a concepção dos Estudos Culturais Físicos (ECF), advém de diversos acontecimentos históricos e sociais que possibilitaram a criação de uma nova abordagem na produção de conhecimento da Sociologia do Esporte norte-americana e que compreende a subjetividade do corpo/sujeito além da sua capacidade de performance no esporte/cultura física.

Resumo Este artigo explora o Physical Cultural Studies (PCS) como potente campo de investigação relacionado ao corpo e à cultura física, desenvolvido no Brasil por pesquisadores/as em contextos locais. O texto aborda o surgimento do PCS como desdobramento do Cultural Studies, as lutas disciplinares nos departamentos de cinesiologia norte-americanos e a virada para a cultura física no interior da sociologia do esporte. Ao mesmo tempo, o estudo discute o PCS a partir das dimensões empírica, contextual, transdisciplinar, teórica, política, qualitativa, autorreflexiva e pedagógica, articulando-as a práticas locais de pesquisa e de intervenção de pesquisadores/as brasileiros/as. Por fim, o estudo lança desafios à construção de um PCS com tons brasileiros, quiçá, latino-americanos.

Human motion tracking is a valuable task for many medical applications. Marker-based optoelectronic systems are considered the gold standard in human motion tracking. However, their use is not always feasible in clinics and industrial environments. On the other hand, marker-less sensors became valuable tools, as they are inexpensive, noninvasive and easy to use. However, their accuracy can depend on many factors including sensor positioning, light conditions and body occlusions. In this study, following previous works on the feasibility of marker-less systems for human motion monitoring, we investigate the performance of the Microsoft Azure Kinect sensor in computing kinematic and dynamic measurements of static postures and dynamic movements. According to our knowledge, it is the first time that this sensor is compared with a Vicon marker-based system to assess the best camera positioning while observing the upper body part movements of people performing several tasks. Twenty-five healthy volunteers were monitored to evaluate the effects of the several testing conditions, including the Azure Kinect positions, the light conditions, and lower limbs occlusions, on the tracking accuracy of kinematic, dynamic, and motor control parameters. From the statistical analysis of the performed measurements, the camera in the frontal position was the most reliable, the lighting conditions had almost no effects on the tracking accuracy, while the lower limbs occlusion worsened the accuracy of the upper limbs. The assessment of human static postures and dynamic movements based on experimental data proves the feasibility of applying the Azure Kinect to the biomechanical monitoring of human motion in several fields.

Globally, systems have invested in a variety of dementia care programs in response to the aging population and those who have been diagnosed with dementia. This study is a qualitative secondary analysis of interview data from a larger study investigating stakeholder perceptions of programs that support caregivers and people living with an Alzheimer's Disease or Alzheimer's Disease-related dementia (AD/ADRD) in five North American jurisdictions. This study analyzed interviews with individuals living with an AD/ADRD and caregivers of individuals living with an AD/ADRD (n = 11). Thematic analysis was conducted to understand how the perception of dementia may have shaped their engagement and experience with healthcare systems. Our analysis resulted in three main themes of care users’ experience: (i) undesirable experience owing to the overarching negative shared understanding and stereotyping of dementia; (ii) dismissal throughout disease progression when seeking health and social care support; and (iii) dehumanization during care interactions. The findings carry critical social and clinical implications, for example, in informing person-centered approaches to care, and communication tools clinicians can use to enhance provider, patient, and caregiver well-being.

Na Leucemia Mieloide Crônica (LMC), pacientes que alcançam resposta molecular profunda sustentada (RMP) com inibidor de tirosinoquinase (ITQ) podem ter o tratamento descontinuado. A remissão livre de tratamento (RLT) ocorre na minoria dos pacientes e não há biomarcadores preditores de manutenção ou perda de resposta. A imunidade antitumoral mediada por Linfócitos T (LT) e Natural Killer (NK) pode contribuir para manutenção e resultar em respostas distintas durante e após a descontinuação. Correlacionamos o fenótipo T e NK com a cinética de doença residual mensurável (uso de ITQ) e com a perda da RLT (descontinuação-DES). Foram incluídos 10 controles saudáveis (CT) e 23 pacientes com LMC: 8 ao diagnóstico (DX), 4 tratados por mais de 3 anos com RM menor que log 3 (RM4,5) em DES, divididos e acompanhados em meses (m), no qual, 3m (n = 13), 6m (n = 8) e 9m (n = 7), e 8 que recaíram (R) no DES. Até o ponto 6m, os pacientes estavam com 50% da dose do ITQ e, após este ponto, tiveram o ITQ suspenso. Foram avaliados frequência, subtipos e maturação de NK e LT por citometria de fluxo do sangue periférico. Os subtipos NK (CD45hiCD19−CD3−) foram definidos como secretório (CD56brightCD16−) ou citotóxico (CD56dimCD16+), e a maturação funcional das células CD19−CD3−CD56+ foi classificada: CD11b−CD27− (tolerante); CD27+CD11b− (secretória imatura); CD27+CD11b+(secretória madura) e CD11b+CD27− (citotóxica), além dos marcadores de ativação CD57 e NKp80. A frequência destas subpopulações foi avaliada em subgrupos de pacientes de acordo com o nível de RM por RT-qPCR para o gene BCR::ABL1. Em comparação aos CT, amostras de LMC em atividade (DX, RM0,05). Em relação aos subtipos de NK, DX, RM 0,05). Na maturação funcional de NK, DX, RM 0,05). Na ativação de NK, DX, RM4,5 e DES, possuem perfil mais maduro de NK, no qual a atividade citotóxica é semelhante aos CT. Entretanto, pacientes maus respondedores (RM

O monitoramento da Leucemia Mieloide Aguda (LMA) é uma estratégia de avaliação prognóstica para identificar Doença Residual Mensurável (DRM). Indica-se o estudo de DRM por métodos moleculares em casos com mutações NPM1 /translocações tipo core-binding fator; mas a citometria de fluxo multiparamétrica (CFMP) é aplicável a mais de 90% das LMAs e preditora do sucesso terapêutico. Diante da escassez no acesso a métodos moleculares em países em desenvolvimento, um protocolo de determinação de DRM na rotina laboratorial é uma necessidade não atendida. Objetivamos implantar um protocolo de DRM por CFMP para LMA e avaliá-lo quanto à sua eficiência e como fator prognóstico adicional à estratificação de risco clássica. Foram incluídos 191 pacientes com LMA de novo ou secundária à síndrome mielodisplásica, entre 18 e 65 anos (09/2015 a 02/2023), com remissão morfológica completa após tratamento com 1 ou 2 ciclos de indução (ciclo 1:3 dias de daunorrubicina 60 mg/m2 e 7 dias de citarabina 200 mg/m2; ciclo 2:3 dias de daunorrubicina 60 mg/m2 e 6 dias de citarabina 1 g/m2 2x/dia). Na Consolidação foi administrada no ciclo1:citarabina 1 g/m2 2x/dia, 6 dias e no ciclo2: idem 1 ou transplante de Medula Óssea (MO) autólogo com condicionamento BuCy. Os pacientes foram estratificados (ELN2017*-exceto mutações TP53 ou ASXL1) de acordo com cariótipo, testes moleculares e coleta de dados clínicos. As DRMs 1, 2 (Pós-indução) e 4 (Pós consolidação), foram avaliadas com: CD45, CD34, CD117, CD33, CD38, CD19, CD123, CD7, CD56, CD13, CD11b, CD14, CD64, CD300e, CD15, CD133 e HLA-DR. Utilizamos a abordagem de LAIPs e DfN, comparando dados de MO saudável e diagnóstico e sendo DRM+ a frequência de células em CD45+ ≥ 0,1% (mínimo 1 fenótipo anormal). Analisamos Sobrevida Global e Livre de Recaída (SG e SLR) e Incidência Cumulativa de Recaída (ICR) - software R (α = 5%). Não foram encontradas associações entre DRM+ e idade, leucócitos, % de blastos, subtipo morfológico e alterações citogenéticas. Observou-se 21% de DRM+ na DRM1,13% na DRM2 e 12,5 % na DRM4. Na DRM1, a frequência de DRM+ para pacientes de risco baixo, intermediário e alto foi: 46,5%, 33,4%, 20,1% e nas DRMs 2 e 4, 33,4%/ 38,9%/ 27,7% e 48,8%/ 48,8%/ 14,4%, respectivamente. Globalmente as DRMs foram definidas como LAIPs ou DfN em 72,3% e 27,7% das amostras. A expressão de CD56 foi a mais frequente (22%), seguida por alterações em CD34/CD13 (21%) e CD33 (17%). DRMsnegativas foram mais frequentes em pacientes com NPM1mut (DRM1, 69,7%) do que em FLT3-ITD (15,2%). A DRM+ após o 2ºciclo de Indução implicou em maior risco de óbito (HR = 1329 (IC;75,16-235022), p = 0,002), maior ICR (44,5% em 30 meses, p = 0,033) e menor SG (4,4 meses, p = 0,018) no risco intermediário. A detecção de DRM+ não se associou aos riscos favorável e alto. Nossos achados demonstram a padronização da DRM por CFMP em uma população brasileira com tratamento único reprodutível usando painel racional com número limitado de marcadores. A detecção de fenótipos ao diagnóstico (LAIP) é mais útil do que os fenótipos semelhantes a DfN, embora sua combinação tenha validado o tubo proposto como “consenso”, aplicável a todos os casos a despeito do fenótipo inicial. Em pacientes de risco intermediário, o resultado da DRM por CFMP se mostrou fator de risco independente para SG. Sendo este grupo correspondente à cerca 1/3 dos casos, comumente com cariótipo normal e desfechos variáveis, a identificação de fatores prognósticos adicionais e dinâmicos tem grande utilidade e pode guiar as decisões terapêuticas. Por fim, a identificação dos fenótipos de DRM definidos neste protocolo pode contribuir na busca por alvos terapêuticos para erradicação das células leucêmicas resistentes.

A Leucemia Mieloide Aguda (LMA) se origina de anormalidades genéticas ao nível das células-tronco hematopoéticas (CTH), que adquirem propriedades de células-tronco leucêmicas (CTL) com capacidade aumentada de sobrevivência e bloqueio de maturação. Hipotetizamos que a parada de maturação leucêmica por meio de definição fenotípica das células de origem possa acrescentar informações importantes sobre a biologia da LMA e contribuir para a predição de desfechos. Assim, analisamos retrospectivamente em coorte brasileira de pacientes com LMA o perfil fenotípico de maturação de blastos leucêmicos e sua associação com achados clássicos de morfologia, risco genético e informações clínicas. Foram incluídos dados de imunofenotipagem de 359 indivíduos com LMA encaminhados para o estudo ICAML2015, entre 18 e 70 anos, acompanhados durante oito anos, exceto os casos de Leucemia Promielocítica Aguda (LPA) que só foram incluídos após 2020. A expressão de CD34, CD117, CD13, CD33, CD38, (c)MPO e HLA-DR foi avaliada para a categorização nos subtipos HSC: Hematopoietic stem cell; MPP: Multipotent progenitors; CMP: Common myeloid progenitors; GMP: Granulocyte-monocyte progenitors; MP: Monocyte progenitors; GP: Granulocyte progenitors. LMAs tipo HSC foram definidas por CD34+, CD117−/+, HLA-DR+, CD13/33−, MPO−. A expressão de CD13 e 33 definiu o subtipo MPP. A expressão de cMPO entre 10-70% definiu o subtipo CMP e > 70% GMP. Os subtipos MP/GP foram diferenciados pela expressão de HLA-DR no subtipo GP. A reclassificação dos casos demonstrou a seguinte distribuição: HSC (n = 31, 8.64%); MPP (n = 77, 21.45%); CMP (n = 37, 10.31%); GMP (n = 75, 20.89%); MP (n = 50, 13.93%); GP (n = 89, 24.79%). De acordo com a estratificação de risco da European-Leukemia Net 2017, dentre os subtipos HSC, MPP, CMP e GMP predominaram o risco favorável, seguidos de intermediário e adverso. Nos subtipos MP e GP, o risco intermediário foi mais prevalente em relação ao favorável e adverso. A correlação com a classificação morfológica demonstrou que LMA sem maturação, com maturação, mielomonocítica e monocítica se distribuíram de forma equivalente nos subtipos MPP, CMP, GMP, MP e GP; no subtipo HSC não se encontrou leucemia monocítica ou promielocítica, sendo esta caracteristicamente do tipo GP e raramente GMP. Para 325 pacientes, os dados dos genes NPM1, FLT3 -ITD, BAALC, EVI1 e CEBPA estavam disponíveis, sendo encontrada com maior frequência a mutação FLT3 -ITD no subtipo MPP (26/325) e NPM1 em GP (44/325). Em algum ponto de seu seguimento 57/359 pacientes recaíram, representando nos subtipos: HSC (5/31, 16%), MPP (15/77, 19%), CMP (6/37, 16%), GMP (16/75, 21%), MP (6/50, 12%) e GP (9/89, 10%). A sobrevida global foi avaliada em 309/359 pacientes, tendo-se observado maior taxa para o subtipo GP (66%) e menor para CMP (11%) aos 24 meses de seguimento (p = 0.03). Os achados apontam que as correlações entre o estado de parada de maturação da LMA e dados clínicos de estratificação de risco e sobrevida merecem atenção e melhor exploração. É possível que a combinação dos dados genéticos com a classificação imunofenotípica de origem leucêmica seja útil para aferir prognóstico na LMA, pois é plausível que as leucemias mais maduras tenham um microambiente inflamatorio exuberante, capaz de modificar a imunidade antitumoral e contribuir para os desfechos diversos.

A Leucemia Linfoblástica Aguda (LLA) é uma neoplasia de células linfoides imaturas T ou B, sendo a segunda leucemia aguda mais comum no adulto. Acomete cerca de 1 a 5 /100.000 indivíduos por ano nos Estados Unidos e cerca de 66% dos casos são LLA-B. O tratamento da LLA-B consiste em quimio/imunoterapia de alta intensidade, com objetivo primário de restaurar a hematopoese normal. A monitorização de remissão de doença (Doença Residual Mensurável - DRM) é realizada em diferentes etapas do tratamento por citometria de fluxo multiparamétrica (CFMP), sendo a técnica eficaz na detecção de clones leucêmicos pós-tratamento com quimioterapia de alta intensidade. Para pacientes recaídos/refratários a terapia de resgate mais indicada consiste em imunoterapia com agentes específicos, chamados de terapias-alvo (ex: anti-CD3 + anti-CD19 -Blinatumomabe, anti-CD22 -Inotuzumabe + Ozogamicin), e a terapia com células T geneticamente modificadas - CAR-T (do inglês, Chimeric antigen receptor T Cell). Com o surgimento de tais tratamentos surge também a demanda por padronização da monitorização de DRM nos pacientes, visto que terapia pode resultar em perda de expressão de marcadores, o que inviabiliza a análise pelos métodos anteriormente padronizados, enfatizando a possibilidade de recaídas com clones emergentes negativos para a expressão de CD19, por exemplo. Assim, foi objetivo do presente estudo determinar a viabilidade de implantação de novo protocolo para detecção de DRM com base em seleção alternativa de antígenos de células B para pacientes submetidos à terapia convencional ou anti-CD19 por CFMP. Foram analisadas as medulas ósseas de 2 doadores saudáveis, e 2 pacientes LLA-B tratados no Hospital das Clínicas de Ribeirão Preto - FMRP-USP, sendo um submetido à terapia HyperCVAD e 1 paciente refratário, tratado com CAR-T anti-CD19. As amostras foram preparadas de acordo com protocolo Euroflow e avaliadas quanto à expressão dos marcadores (CD45, CD34, CD38, CD81, CD10, CD20, CD19, CD22, CD24, CD66b, CD66c + CD123, CD73) por CFMP em citômetro BD Canto II e analisadas em software Infinicyt por dois observadores diferentes. Os resultados da análise DRM foram expressos como a porcentagem de células nucleadas viáveis que abrigam o fenótipo anormal, sendo a positividade definida quando igual ou maior do que 0,01%. No presente estudo de viabilidade a detecção de células B foi concordante entre os diferentes tubos, independente de marcação de CD19, não havendo diferença nas frequências observadas tanto para amostras de referência como no paciente analisado (p = 0,4478, Kruskal-Wallis). Não foi observada diferença entre as frequências de células anormais detectadas pelos observadores (p = 0,4, Kruskal Wallis) e nossos resultados demonstram reprodutibilidade entre as análises realizadas, com Correlação de Pearson calculada de 0,9995 (IC: 0,9949-0,999, r2 = 0,9989). A análise de Bland-Altman demonstrou não haver divergência entre as metodologias utilizadas. Não houve diferença nas frequências obtidas dos fenótipos caracterizados como DRM+, independente da combinação de marcadores para a gate de células B (CD19xCD22, CD22xCD24, e CD24xCD66b) p = 0,667, Friedman, α=0,05). Em conclusão, nossos dados indicam que o monitoramento da DRM para pacientes com LLA-B tratados com imunoterapia anti-CD19 é possível por CFMP resultando em dados altamente concordantes. O desempenho do resultado preliminar exposto deve garantir resultados comparáveis ao serem avaliados em múltiplos centros, etapa em fase de planejamento, para garantir o avanço da padronização da resposta de DRM por CFMP.

This research aims to obtain facts, data, and information about the results of the evaluation of the achievement coaching program for the women's softball sports of DKI Jakarta Province. This study used evaluative methods with the CIPP model. Data collection techniques use questionnaires, documentation, observations, and interviews. The subjects of this study are The Core Board, Coach, and Athlete. The results revealed that the context component of the Pengprov Perbasasi DKI Jakarta already has a long-term, medium, and short-term plan. However, the DKI Jakarta Provincial Women's Softball Team was only able to achieve the goal of the achievement coaching program at the 2018 Softball National Tournament. The input components, facilities, equipment, and equipment provided are enough to meet the needs of the DKI Jakarta Provincial Women's Softball Team. However, the need for supplements and vitamins for new athletes ahead of the implementation of PON. In the process component, the coaching staff implements a walking selection system and promotes and degrades athletes based on batting average data, left on base, running base in, accumulation of errors, & success in sacrifice. The training process was carried out face-to-face and virtually during the Covid-19 pandemic. In the product component, the results obtained by the DKI Jakarta Provincial Women's Softball Team during the period 2018 to 2022 were finalists in the 2018 Softball National Tournament. However, in the 2019 PON Qualification and PON XX Papua in 2021, the DKI Jakarta Provincial Women's Softball Team was only able to rank fifth

Abstract How does urban expansion interact with pastoral climate change adaptation? This article explores pastoral adaptation strategies at the rural-urban interface. It examines how Maasai pastoralists in peri-urban Nairobi, Kenya, respond to climate hazards in the context of urban expansion, land use change, and land privatisation. Using mixed-method research, the study is informed by a household survey (n = 72), 38 qualitative interviews, and 12 focus group discussions. Drawing on the literature on climate change adaptation, pastoral change, and peri-urban dynamics, we find that while urban expansion provides significant challenges for pastoral livelihoods in the study area, pastoralists also engage new opportunities in the peri-urban context and employ them in their adaptation strategies. We show how adaptation strategies related to mobility, diversification, market exchange, and storage are employed through a variety of efforts including engagement with urban land markets and demand for livestock products, and by capitalising on proximity to transport, trading facilities, water, and commercial fodder. Communal pooling, another adaptation strategy, is less used and perceived to be in decline. We further find that historical land ownership patterns play a significant role in adaptation strategies, as pastoralists who have benefitted from rangeland privatisation are able to convert high peri-urban land values into private rural land access and investments in, e.g. diversification. Poor households are in a more precarious position but draw on informal agreements to access land as part of their adaptation strategies. Our findings highlight how pastoral households at the rural-urban interface may draw actively on peri-urban opportunities in their adaptation strategies as part of their efforts to enhance livelihoods, and in so doing bridge peri-urban and rural space. More broadly, our study highlights the importance of understanding pastoral climate change adaptation in the context of wider changes in livelihoods, land use, and land rights, rather than as isolated actions. Graphical Abstract

Estimating future short-duration extreme precipitation in mountainous regions is fundamental for risk management. High-resolution convection-permitting models (CPMs) represent the state of the art for these projections, as they resolve convective processes that are key to short-duration extremes. Recent observational studies reported a decrease in the intensity of extreme hourly precipitation with elevation. This “reverse orographic effect” could be related to processes which are subgrid even for CPMs. To quantify the reliability of future projections of extreme short-duration precipitation in mountainous regions, it is thus crucial to understand to what extent CPMs can reproduce this effect. Due to the computational demands however, CPM simulations are still too short for analyzing extremes using conventional methods. We use a non-asymptotic statistical approach (Simplified Metastatistical Extreme Value: SMEV) for the analysis of extremes from short time periods, such as the ones of CPM simulations. We analyze an ERA-Interim-driven Consortium for Small-Scale Modeling (COSMO-crCLIM, convection-resolving Climate Modelling) simulation (2000–2009; 2.2 km resolution), and we use hourly precipitation from 174 rain gauges in an orographically complex area in northeastern Italy as a benchmark. We investigate the ability of the model to simulate the orographic effect on short-duration precipitation extremes, as compared to observational data. We focus on extremes as high as the 20-year return levels. While overall good agreement is reported at daily and hourly duration, the CPM tends to increasingly overestimate hourly extremes with increasing elevation, implying that the reverse orographic effect is not fully captured. These findings suggest that CPM bias-correction approaches should account for orography. SMEV's capability of estimating reliable rare extremes from short periods promises further applications on short-time-period CPM projections and model ensembles.

This study aims to determine the influence of learning media and learning motivation on the learning outcomes of short-distance running. This research was conducted at SMPN 15 Depok class VII. This research method uses experimental research with a treatment design by level 2x2. The sample in this study consisted of 60 people. Sample collection using simple random sampling technique. The data analysis technique is two-path variance analysis (ANAVA) and is continued with the Tukey test at a significant level ɑ = 0.05. To test the normality of the data, use the kolmogorv-smirnov test and the homogeneity test using the levene test. The results of this study show that (1) Overall visual learning media is better than the use of audio learning media based on the value of F (OA) = 21.11 with p-value = 0.000 < 0.05. (2) There is an influence of interaction between learning media and motivation on short-distance running learning outcomes based on F (OAB) value = 11.128 with p-value = 0.002 < 0.05. (3) Learning outcomes of short-distance running with visual learning media that have higher learning motivation than children learning with audio learning media who have high learning motivation based on Qcount = 15.01 > Qtabel (0.05;4:30) = 3.85. (4) Learning outcomes of short-distance running with visual media and audio media on low learning motivation there was no noticeable or significant difference based on the value of Qcount = 2.39 < Qtabel (0.05;4:30) = 3.85.