1. Abolishing Retro-Transduction of Producer Cells in Lentiviral Vector Manufacturing
- Author
-
Soledad Banos-Mateos, Carlos Lopez-Robles, María Eugenia Yubero, Aroa Jurado, Ane Arbelaiz-Sarasola, Andrés Lamsfus-Calle, Ane Arrasate, Carmen Albo, Juan Carlos Ramírez, and Marie J. Fertin
- Subjects
lentiviral vector ,VSV-G pseudotyping ,LDL receptor ,retro-transduction ,gene therapy ,lentiviral vector manufacturing ,Microbiology ,QR1-502 - Abstract
Transduction of producer cells during lentiviral vector (LVV) production causes the loss of 70–90% of viable particles. This process is called retro-transduction and it is a consequence of the interaction between the LVV envelope protein, VSV-G, and the LDL receptor located on the producer cell membrane, allowing lentiviral vector transduction. Avoiding retro-transduction in LVV manufacturing is crucial to improve net production and, therefore, the efficiency of the production process. Here, we describe a method for quantifying the transduction of producer cells and three different strategies that, focused on the interaction between VSV-G and the LDLR, aim to reduce retro-transduction.
- Published
- 2024
- Full Text
- View/download PDF