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Spontaneous invasive non-inflammatory canine mammary carcinomas (CMC) and their regional lymph nodes (LN) were analysed (n = 136). Histological grade (HG) and vascular invasion (VI) in the tumours and lymph node status were recorded. Proliferation index (PI), microvessel density (MVD) and vascular endothelial growth factor receptor 2 (VEGFR2) expression were estimated using anti-proliferating cell nuclear antigen (PCNA), anti-von Willebrand factor and anti-Flk-1, respectively. Eighteen months follow-up was performed (34 bitches). Tumours of different grades showed differences regarding PI, Flk-1/integrated optical density (Flk-1/IOD) and MVD. Every feature showed significant association with LN status through bivariate analyses. From multivariate analyses, VI and Flk-1/IOD were selected to predict LN status. Data revealed that the probability of a CMC-bearing bitch to remain alive at 1, 4, 5 and 14–18 months was 0.91, 0.87, 0.81 and 0.77, respectively. Besides LN status, VI was the only feature positively correlated with survival time, although a trend to shorter survival of animal patients bearing high expressing VEGFR2 CMC was noted. Fil: Diessler, Mónica Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas; Argentina Fil: Castellano, M.C.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina Fil: Portiansky, Enrique Leo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología General Veterinaria; Argentina Fil: Burns, Sarah. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Laboratorio de Investigación en Sistemas Ecológicos y Ambientales; Argentina Fil: Idiart, Julio Roberto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología Especial; Argentina

A 10-year-old male Briard dog was presented because of listlessness, abnormal gait, fever, inappetence, and seizures. A non-pigmented growth was observed in the ventral quadrant of the left iris. Thoracic radiographs revealed multiple pulmonary metastases and the owner opted for killing. On necropsy, lung masses and nodules in left iris, right adrenal medulla, and brain were detected. Histologically the primary tumour was diagnosed as pulmonary adenocarcinoma with predominant solid pattern. Metastases to regional lymph nodes, uvea, adrenal medulla, and brain were recognized. The metastatic behaviour resembled that occurring in humans. To the authors' knowledge, this is the first report of a pulmonary adenocarcinoma with metastasis to the uvea in a dog.

Lymphangiosarcoma is a rare tumour in domestic animals arising from lymphatic endothelial cells. Occasionally, microscopic differentiation with haemangiosarcoma may be difficult. The aim of the present study was to describe a lymphangiosarcoma in a 1-year-old female Doberman Pinscher dog and to characterize its lectinhistochemical binding pattern as compared with that of haemangiosarcoma. The dog was presented because of a cutaneous painful swelling located in the left axilla. Histological diagnosis confirmed lymphangiosarcoma. The dog was killed. Necropsy revealed mediastinal lymph nodes' involvement. Twenty lectins were tested in tissue sections of this case as well as in four haemangiosarcomas from other dogs. Staining intensity was issued upon optical density determinations. Percentage of lectinhistochemical staining area was also conducted. RCA-I showed the most intense and wide distributed labelling pattern for lymphangiosarcoma. PHA-E was the counterpart for haemangiosarcoma. Should similar results be obtained in further studies, such differences could aid in the differential diagnosis between lymphangiosarcoma and haemangiosarcoma when histological pictures were not conclusive.

Se Utilizaron 100 sueros de gatos provenientes de la ciudad de La Plata y alrededores con el fin de determinar la relación entre la infección con FIV y la infección con T. gondii. Los sueros se analizaron para determinar anticuerpos anti-FIV y anti-T.gondii por las pruebas de <> e inmunofluorescencia indirecta, respectivamente. Para el análisis de los resultados los sueros se clasificaron en cuatro grupos según presentaran o no anticuerpos anti-FIV y anti-T. gondii (Tox): Grupo 1) FIV+ Tox+; Grupo 2) FIV+ Tox-; Grupo 3) FIV- Tox+; Grupo 4) FIV- Tox-. Se establecieron tres categorías por grupo con respecto a los signos clínicos de los gatos estudiados. El por- centaje de sueros positivas a FIV y a T. gondii (34,5%, 19,) fue significativamente mayor que el porcentaje de sueros positivos a FIV y nagativos a T. gondii (17,7%, 8/45; p < 0,05, prueba exacta de Fisher). Los títulos de anticuerpos anti-T. gondii fueron siqnificativamente mayores en el Grupo 1 con respecto al Grupo 3. La distribución de los signos clínicos fue significativamente diferente entre los 4 grupos. El porcentaje de animales con signos clínicos potencialmente compatibles con FIV y/o toxoplasmosis fue: Grupo 1: 57,9%, , Grupo 2: 75%; Grupo 3: 48,6% y Grupo 4: 27%. Los resultados del presente trabajo indican la asociación entre la infección con el FIV y la infección con T.gondii Serum samples from 100 cats from La Plata and surroundings were studied to determine the relation between FIV infection and Toxoplasma gondii infection. Serum samples were analized to determine anti-FIV and anti-T. gondii antibodies, using immunoblotting test and immunofluorescence test, respectively. To analize the results, serum samples were classified into 4 groups according to the presence or not of anti-FIV and anti-T. gondii antibodies (Tox): Group 1) FIV+; Tox+ Group 2) FIV+ Tox-; Group 3) FIV- Tox+; Group 4) FIV- Tox-. Each group was further divided into 3 categories according clinical signs. The percentage of sera positive for FIV and T. gondii (34.5%, 19/55) was significantly higher than the percentage of sera positive for FIV and negative for T. gondii (17.7%, 8/45; p < 0.05, Fisher exact test). Antibodies tires anti-T. gondii were significantly higher in group 1 than in group 3. Distribution of clinical signs demonstrated significant differences between groups. The percentage of animals with clinical signs consistent with FIV and/or. T. gondii infections were as follows: Group 1: 57.9%; Group 2; 75%, Group 3: 48.6% and Graup 4: 27%. Results the present study indicate the association between FIV infection and T. gondii infection

Conventional methods used in laboratory animals for collection of resident macrophages (MAC) proved suitable for isolation of hepatic, splenic, dermic, peritoneal and alveolar MAC of healthy dogs. Phagocytic assays of IgG opsonised SRBC were performed with resident MAC obtained from these organs. Results showed the existence of statistically significant differences among phagocytic capacity of the investigated MAC. According to this, three groups of MAC could be defined: a first group comprising dermic, splenic and alveolar MAC; a second group comprising hepatic MAC and a third group comprising peritoneal MAC.