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Land surface temperature (LST) serves as a pivotal component within the surface energy cycle, offering fundamental insights for the investigation of agricultural water environment, urban thermal environment, and land planning. However, LST monitoring at a point scale entails substantial costs and poses implementation challenges. Moreover, the existing LST products are constrained by their low spatiotemporal resolution, limiting their broader applicability. The fusion of multi-source remote sensing data offers a viable solution to enhance spatiotemporal resolution. In this study, the Enhanced Spatial and Temporal Adaptive Reflectance Fusion Model (ESTARFM) was used to estimate time series LST utilizing multi-temporal Landsat 8 (L8) and MOD21A2 within the Haihe basin in 2021. Validation of ESTARFM LST was conducted against L8 LST and in situ LST. The results can be summarized as follows: (1) ESTARFM was found to be effective in heterogeneous regions within the Haihe basin, yielding LST with a spatiotemporal resolution of 30 m and 8 d while retaining clear texture information; (2) the comparison between ESTARFM LST and L8 LST shows a coefficient determination (R2) exceeding 0.59, a mean absolute error (MAE) lower than 2.43 K, and a root mean square error (RMSE) lower than 2.63 K for most dates; (3) comparison between ESTARFM LST and in situ LST showcased high validation accuracy, revealing a R2 of 0.87, a MAE of 2.27 K, and a RMSE of 4.12 K. The estimated time series LST exhibited notable reliability and robustness. This study introduced ESTARFM for LST estimation, achieving satisfactory outcomes. The findings offer a valuable reference for other regions to generate LST data with a spatiotemporal resolution of 8 d and 30 m, thereby enhancing the application of data products in agriculture and hydrology contexts.