34 results on '"Maohong Zhang"'
Search Results
2. Research on Security and Privacy of Big Data under Cloud Computing Environment.
- Author
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Maohong Zhang, Aihua Yang, and Hui Liu
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- 2018
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3. The Quality Assessment of Student Learning Based on Cloud Model.
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Lizhen Liu, Wentao Wang, and Maohong Zhang
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- 2012
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4. Severity stratification of aplastic anemia
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Cuiai, Ren, Yanxiang, Li, Jingying, Cui, Fengxia, Liu, Zhixin, Sheng, Wenjun, Xu, and Maohong, Zhang
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- 2014
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5. The Construction and Application of Hybrid Teaching Model of Flipping Classroom and Divided Classroom Base on Project-driven in the Teaching of Java programming
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Yuan Liu, Xueyan Gong, Yuhong Sun, Zhenmei Yu, and Maohong Zhang
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History ,Java ,business.industry ,Computer science ,ComputingMilieux_COMPUTERSANDEDUCATION ,Project driven ,Software engineering ,business ,Base (topology) ,computer ,Computer Science Applications ,Education ,computer.programming_language - Abstract
In view of the problems existing in the teaching of Java programming, in order to further improve the teaching quality of Java programming, a hybrid teaching model of flipping classroom and divided classroom based on project-driven was proposed. The problem-oriented, student-cantered teaching concept forms an effective teaching activity for teachers and students. With a complete project throughout the course, online learning is adopted to deeply explore students’ independent learning ability, assist the teaching of course content and group communication learning, combine the flipped classroom with the divided classroom to construct a hybrid teaching model. The experiment proves that under the project-driven teaching mode, the full use of the hybrid teaching of the flipped classroom and the divided classroom can greatly improve learning effect of students at different levels of knowledge, and students’ independent learning ability, innovation and creativity ability can also be improved accordingly.
- Published
- 2020
6. Research on Topic Mining Algorithm Based on Deep Learning Extension
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Xuechen Zhao, Fuqiang Yang, and Maohong Zhang
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History ,Computer science ,business.industry ,Deep learning ,Topic mining ,Artificial intelligence ,Extension (predicate logic) ,Machine learning ,computer.software_genre ,business ,computer ,Computer Science Applications ,Education - Abstract
This paper proposes a new algorithm that can consider both keywords and time of occurrence. Firstly, after the data is preprocessed, the LDA model of the theme event set is established, and the set of subject words is generated as the description mark of the event. The semantic and temporal similarity between the event keywords are calculated by the DTW algorithm to obtain the corresponding similarity matrix. Finally, the collaborative training method is used to iteratively generate the final feature vector and complete the event selection. The simulation results show that the proposed algorithm has higher accuracy and higher efficiency than previous algorithms.
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- 2019
7. Research on the Theory of Multiple Intelligences in Training Mode of College Students’ Innovative Ability
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Hui Liu, Lihua Sun, and Maohong Zhang
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History ,media_common.quotation_subject ,Theory of multiple intelligences ,Interpersonal communication ,Creativity ,Computer Science Applications ,Education ,Scientific literacy ,ComputingMilieux_COMPUTERSANDEDUCATION ,Mathematics education ,Introspection ,Quality (business) ,Meaning (existential) ,Psychology ,media_common ,Computer technology - Abstract
In today’s pluralistic era, the cultivation of comprehensive quality education is being paid more and more attention. With the development of computer technology and artificial intelligence, especially with the advent of the theory of multiple intelligences, the meaning of intelligence is being understood again, provide the feasible thought guidance for the implementation of comprehensive quality education. Applying the theory of multiple intelligences to the subject teaching, the teaching design is aimed at the students’ individual intelligence advantage. In the course of teaching, we should pay attention to the use of weak intelligence such as interpersonal communication intelligence and introspection intelligence, in order to promote the all-round, active and individual development of students’ multiple intelligences. Through the preliminary experiment exploration on the improvement of teaching quality, we obtain the cultivation mode of college students’ innovative ability based on the theory of multiple intelligences after theoretical analysis and experimental verification. It is conducive to create suitable environment to develop multiple intelligence for students, so that students’ comprehensive intelligence, innovation consciousness and personality potential can be fully developed, so as to promote the cultivation of College Students’ creativity, stimulation of creativity and speed of scientific literacy.
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- 2019
8. Research on Security and Privacy of Big Data under Cloud Computing Environment
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Maohong, Zhang, primary, Aihua, Yang, additional, and Hui, Liu, additional
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- 2018
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- View/download PDF
9. Research on Security and Privacy of Big Data under Cloud Computing Environment.
- Author
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Maohong, Zhang, Aihua, Yang, and Hui, Liu
- Published
- 2018
- Full Text
- View/download PDF
10. Expression of survivin and bax/bcl-2 in peroxisome proliferator activated receptor-γ ligands induces apoptosis on human myeloid leukemia cells in vitro
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Yong Song, Feng Chen, Jia-Jun Liu, Ming Hou, Ren-Wei Huang, Jun Peng, Maohong Zhang, Dong-Jun Lin, X. Wu, Qu Lin, and Xinliang Pan
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Programmed cell death ,Survivin ,Down-Regulation ,Antineoplastic Agents ,Apoptosis ,HL-60 Cells ,Cysteine Proteinase Inhibitors ,Biology ,Ligands ,Inhibitor of Apoptosis Proteins ,Troglitazone ,Downregulation and upregulation ,medicine ,Humans ,Immunologic Factors ,Chromans ,Fragmentation (cell biology) ,bcl-2-Associated X Protein ,Prostaglandin D2 ,Reverse Transcriptase Polymerase Chain Reaction ,Myeloid leukemia ,Hematology ,medicine.disease ,Neoplasm Proteins ,Up-Regulation ,Gene Expression Regulation, Neoplastic ,PPAR gamma ,Leukemia ,Proto-Oncogene Proteins c-bcl-2 ,Oncology ,Cancer research ,Thiazolidinediones ,K562 Cells ,Microtubule-Associated Proteins ,K562 cells - Abstract
The present study was undertaken to investigate the mechanisms of peroxisome proliferator activated receptor-gamma (PPAR-gamma) ligand-induced apoptosis on human myeloid leukemia K562 and HL-60 cell lines. The results revealed that both 15-deoxy-delta(12,14)-prostaglandin J2 (15d-PGJ2) and troglitazone (TGZ) have significant anti-proliferation- and apoptosis-inducing effects on these two kinds of leukemia cells. Marked morphological changes of cell apoptosis including condensation of chromatin and nuclear fragmentation were observed clearly using Wright's and Hoechst 33258 staining. Reverse transcription-PCR and western blot analyses demonstrated that both survivin and bcl-2 expression were downregulated markedly, while bax expression was upregulated concurrently when apoptosis occurred. We therefore conclude that 15d-PGJ2 and TGZ have significant apoptosis effects on K562 and HL-60 cells in vitro, and that upregulation of bax as well as downregulation of survivin and bcl-2 expression may be the important apoptosis-inducing mechanisms. The results suggest that PPAR-gamma ligands may serve as potential therapeutic agents for both acute and chronic myeloid leukemia.
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- 2005
11. The TNF2 allele is a risk factor to severe aplastic anemia independent of HLA-DR
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Conggao Xu, Jun Peng, Chuanfang Liu, Yuan-yuan Zhu, Yuan Yu, Jie Li, Kunli Zhu, Xueliang Chen, Ming Hou, and Maohong Zhang
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Adult ,Male ,Adolescent ,Immunology ,Locus (genetics) ,Human leukocyte antigen ,Major histocompatibility complex ,Pathogenesis ,hemic and lymphatic diseases ,HLA-DR ,Humans ,Immunology and Allergy ,Medicine ,Genetic Predisposition to Disease ,Allele ,Aplastic anemia ,Alleles ,biology ,Tumor Necrosis Factor-alpha ,business.industry ,Anemia, Aplastic ,Promoter ,HLA-DR Antigens ,General Medicine ,Middle Aged ,medicine.disease ,stomatognathic diseases ,biology.protein ,Female ,business - Abstract
Severe aplastic anemia (SAA) is a disease with an autoimmune component. The susceptibility to the development of SAA is strongly associated with genes in the major histocompatibility complex (MHC). The gene for tumor necrosis factor-alpha (TNF-alpha) is encoded in the MHC locus and TNF-alpha is involved in the pathogenesis of SAA. A TNF-alpha variant with a polymorphism at position -308 in its promoter region (-308A), which is designated TNF2, has been demonstrated to be linked to a number of autoimmune diseases. In this study, the TNF-alpha -308 promoter polymorphism and HLA-DRB1 alleles were analyzed in 75 SAA patients, 55 mild aplastic anemia patients (MAA), and 128 controls. In SAA the phenotype frequencies of TNF2, HLA-DR3, and -DR2 were significantly higher in comparison to controls. Stratification analysis confirmed that the TNF2 allele contributes to the susceptibility to SAA independently of HLA-DR3 or -DR2. The results indicated that TNF2 might act as an independent risk factor for SAA.
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- 2003
12. Mycophenolate mofetil (MMF) for the treatment of steroid-resistant idiopathic thrombocytopenic purpura
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Ming Hou, Ping Qin, Yan Shi, Jun Peng, Chuanli Zhao, Xueyong Wang, Chun-qing Zhang, Xue-bin Ji, and Maohong Zhang
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medicine.medical_specialty ,Chemotherapy ,Nausea ,business.industry ,medicine.medical_treatment ,Splenectomy ,Immunosuppression ,Hematology ,General Medicine ,medicine.disease ,Gastroenterology ,Thrombocytopenic purpura ,Mycophenolic acid ,Refractory ,Prednisone ,Internal medicine ,Immunology ,medicine ,medicine.symptom ,business ,medicine.drug - Abstract
The treatment of chronic idiopathic thrombocytopenic purpura (ITP) is difficult in those unresponsive to corticosteroids and/or splenectomy. We attempted to induce durable response in 21 patients with refractory ITP by applying mycophenolate mofetil (MMF) (1.5-2.0 g/d), a novel immunosuppressive agent. Overall response rate was 62% (13 of 21), including 24% (five of 21) in complete response (CR), 29% (six of 21) in partial response (PR), and 10% (two of 21) in minor response (MR). The response rates for non-splenectomized and splenectomized ITP patients were 64% (nine of 14) and 57% (four of seven), respectively (P > 0.05). 39% (five of 13) responders relapsed as a result of dose reduction or withdraw of MMF, and 61% (eight of 13) responders maintained their effectiveness for a median of 24 wk. Sustained response was observed in three patients in whom MMF was withdrawn. MMF was well tolerated with only slight nausea and diarrhea recorded in 3 of 21 cases. No premature withdrawal was found in this study. CD3+ peripheral blood mononuclear cells (PBMC) and CD19+ PBMC were significantly reduced 12 wk after MMF administration in the responders. Platelet-associated antibodies against glycoproteins GPIIb/IIIa were detected in 13 of 21 (62%) patients before MMF treatment, and antibody levels were significantly decreased in responders 12 wk after MMF administration. This suggested that MMF might correct the immunologic abnormalities underlying the destruction of circulating platelets in ITP. We conclude that MMF could be used as a second-line agent for the treatment of steroid-resistant ITP before or after splenectomy and thereby is worth of further evaluation in randomized studies.
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- 2003
13. Effects of B7-blocking agent and/or CsA on induction of platelet-specific T-cell anergy in chronic autoimmune thrombocytopenic purpura
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Zhen-Guang Wang, Ming Hou, Wei Li, Xueliang Chen, Nianzeng Xing, Maohong Zhang, Chuanfang Liu, Cui-ai Ren, Conggao Xu, Chunyan Ji, Jun Peng, and Dai Liu
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Adult ,Blood Platelets ,Male ,Interleukin 2 ,Immunoconjugates ,Adolescent ,T-Lymphocytes ,Immunology ,Lymphocyte Activation ,medicine.disease_cause ,Biochemistry ,Autoimmunity ,Immune tolerance ,Abatacept ,Immune system ,Antigen ,Cyclosporin a ,Immune Tolerance ,medicine ,Humans ,Antigens, Human Platelet ,Prospective Studies ,Child ,Clonal Anergy ,Autoimmune disease ,Purpura, Thrombocytopenic, Idiopathic ,business.industry ,Cell Biology ,Hematology ,Middle Aged ,Ciclosporin ,medicine.disease ,Treatment Outcome ,Case-Control Studies ,Chronic Disease ,B7-1 Antigen ,Cyclosporine ,Drug Therapy, Combination ,Female ,business ,medicine.drug - Abstract
Chronic autoimmune thrombocytopenic purpura (AITP) is characterized by platelet-specific autoantibody production that is influenced by enhanced antiplatelet T-helper cell reactivity. Costimulatory signals are absolutely required for T-cell activation and play key roles in the decision between tolerance and immunity. In this study we cultured T cells isolated from patients with chronic AITP to investigate the effects of the B7-blocking agent cytologic T-lymphocyte-associated antigen 4-immunoglobulin (CTLA4-Ig), and cyclosporin A (CsA), alone or in combination, on induction of platelet-specific T-cell anergy. The data showed that in most cases CTLA4-Ig and/or CsA could induce tolerance toward platelet antigens based on anergy. It could be overcome by stimulation with unrelated antigens, demonstrating its platelet specificity. The anergy is associated with lack of interleukin 2 (IL-2) and withheld by exogenous IL-2, emphasizing the pivotal role of IL-2 suppression in the induction of platelet-specific anergy. We also prospectively evaluated the efficacy of CsA therapy in patients with refractory AITP and observed that the response to CsA treatment in vivo was associated with the inhibiting sensitivity of platelet-reactive T cells to CsA in vitro. This suggests that the sensitivity of T cells to CsA in vitro could serve as a reliable parameter in predicting the efficacy of CsA for patients with refractory AITP. CTLA4-Ig may become a promising new therapeutic agent for the treatment of chronic AITP, and the combination of CTLA4-Ig and CsA would be a more powerful strategy for the management of refractory AITP.
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- 2002
14. Imatinib achieved complete cytogenetic response in a CML patient received 32-year indirubin and its derivative treatment
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Chunyan Ji, Feng Chen, Lizhen Li, Daoxin Ma, Ming Hou, Maohong Zhang, Shuxin Yan, and Jian-zhi Sun
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Cancer Research ,business.industry ,Imatinib ,Hematology ,Philadelphia chromosome ,medicine.disease ,chemistry.chemical_compound ,Myelogenous ,Leukemia ,Imatinib mesylate ,Oncology ,chemistry ,medicine ,Cancer research ,Neoplasm ,Indirubin ,business ,Derivative (chemistry) ,medicine.drug - Published
- 2010
15. A case of Philadelphia-chromosome positive chronic idiopathic myelofibrosis
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Feng Chen, Jian-zhi Sun, Chun-qing Zhang, Shuxin Yan, Lizhen Li, Chunyan Ji, Wen Wang, Maohong Zhang, and Ming Hou
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Male ,Cancer Research ,medicine.medical_specialty ,Idiopathic myelofibrosis ,Philadelphia positive ,Philadelphia chromosome ,Gastroenterology ,Piperazines ,hemic and lymphatic diseases ,Internal medicine ,mental disorders ,medicine ,Humans ,Philadelphia Chromosome ,Myeloproliferative Disorders ,Philadelphia Chromosome Positive ,business.industry ,Myeloid leukemia ,Imatinib ,Hematology ,Middle Aged ,medicine.disease ,humanities ,Peripheral blood ,Pyrimidines ,Imatinib mesylate ,Oncology ,Primary Myelofibrosis ,Benzamides ,Chronic Disease ,Imatinib Mesylate ,business ,psychological phenomena and processes ,medicine.drug - Abstract
Philadelphia-chromosome positive thrombocythemia without features of chronic myeloid leukemia in peripheral blood has been described before. However, there are few reports on Philadelphia-chromosome positive chronic idiopathic myelofibrosis. We present a case of Philadelphia positive chronic idiopathic myelofibrosis that had no obvious response to imatinib.
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- 2008
16. Research of mobile learning mode based on WeChat public platform
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Maohong, Zhang, primary, Hui, Liu, additional, Xingzhi, Zhao, additional, Li, Zang, additional, and Xiaoli, Zhu, additional
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- 2016
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17. The Quality Assessment of Student Learning Based on Cloud Model
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Wentao Wang, Maohong Zhang, and Lizhen Liu
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Multimedia ,business.industry ,Process (engineering) ,Computer science ,Quality assessment ,media_common.quotation_subject ,Stability (learning theory) ,Cloud computing ,Division (mathematics) ,computer.software_genre ,Intelligent tutoring system ,Human-Computer Interaction ,Artificial Intelligence ,Test score ,ComputingMilieux_COMPUTERSANDEDUCATION ,Quality (business) ,Artificial intelligence ,Student learning ,business ,computer ,Software ,media_common - Abstract
In traditional tutoring system, the quality assessment of student learning is identified by hard division regardless of the uncertainty of results. In the paper, Cloud M odel T heory was applied to build student model in Intelligent Tutoring System. And a quality assessment approach of student learning based on cloud m odel was proposed. S tudents’ test score s are regarded as cloud drop let s, and data is discret ized according to contribution of the cloud concept in the research . Further more , cloud transform algorithm is introduced to compute membership cloud. Finally, maximum determination algorithm is used to obtain more actual grade division of learning quality. Experimental results show that membership concept can reflect not only t he mastery level of knowledge points but also the stability and psychology in the process of student learning . The study will help improve the efficiency of Intelligent Tutoring System.
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- 2012
18. Non-leukemic myeloid sarcoma involving the vulva, vagina, and cervix: a case report and literature review
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Xuemei Qin, Yanlin Sun, Wenxia Wang, Yuan Yu, Maohong Zhang, and Shuxin Yan
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medicine.medical_specialty ,Pathology ,Myeloid ,medicine.diagnostic_test ,business.industry ,leukemia ,Case Report ,genital tract ,Gene mutation ,chemotherapy ,medicine.disease ,Gastroenterology ,Leukemia ,medicine.anatomical_structure ,Oncology ,Internal medicine ,Biopsy ,myeloid sarcoma ,Myeloid sarcoma ,Medicine ,Pharmacology (medical) ,Bone marrow ,business ,Cervix ,Rare disease - Abstract
Myeloid sarcoma (MS) is defined as a tumor mass consisting of myeloid blast with or without maturation occurring at an anatomical site other than bone marrow with normal architectural effacement. It can also precede the onset of leukemia which is called non-leukemic MS. Non-leukemic MS is a kind of rare disease and easy to be misdiagnosed as other common malignancies due to the rarity and nonspecific manifestation. We herein report an unusual case of non-leukemic MS involving the vulva, vagina, and cervix in a female patient. The bone marrow aspiration and biopsy of the patient revealed no hematological abnormality. Immunohistochemical staining of the biopsies was strongly positive for myeloperoxidase, CD68, leukocyte common antigen (LCA), CD117, CD34, CD38, CD79a, and negative for cytokeratin (CK), epithelial memberane antigen (EMA), CD2, CD3, CD20, CD5, CD138. Then a diagnosis of non-leukemic MS was made. Unfortunately, our patient received only one cycle of chemotherapy consisting of cytosine arabinoside and daunorubicin, then refused any further treatment and died 4 months after diagnosis. Although systemic chemotherapy is widely accepted to be a promising strategy, its benefit still needs to be further assessed. Certain questions still need to be answered for this disease: 1) Why can approximately 20% of the patients with non-leukemic MS remain disease-free after local therapy alone? 2) How many cycles of chemotherapy are needed for these patients after achievement of complete remission? 3) What are the prognostic or risk factors in these patients who have no abnormality of karyotype, fusion genes, or gene mutation to predict responsiveness to chemotherapy and outcome? 4) What is the risk factor for relapse? The rarity of non-leukemic MS makes it almost impossible to conduct large-scale randomized trials, but judicious study for each patient with MS is helpful for a further understanding of the nature of the disease.
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- 2015
19. Notice of Retraction: The research of city community safety management system
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Lizhen Liu, Lei Chen, Maohong Zhang, and Chao Du
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Notice ,business.industry ,Computer science ,media_common.quotation_subject ,Community organization ,Community management ,Public relations ,Democracy ,Politics ,Promotion (rank) ,Work (electrical) ,Management system ,business ,media_common - Abstract
With the development of society, more and more emphasis put on community safety, Community Safety Manager plays an important positive role in maintaining social safety and stability and the promotion of social harmony. Therefore, How to implement the innovative model of community management system, strengthening grass-roots development and democratic self-government building and improve the city economic, political and cultural development needs of communities in public safety management level has become an urgent research issue. In this paper, development and application of safety management system in city communities at home and abroad are summed up, which is based on the city community through the work of safety management system characteristics analyzed, and the establishment of an intelligence-based decision-making process of city community safety management system.
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- 2010
20. Ponicidin, an ent-kaurane diterpenoid derived from a constituent of the herbal supplement PC-SPES, Rabdosia rubescens, induces apoptosis by activation of caspase-3 and mitochondrial events in lung cancer cells in vitro
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Maohong Zhang, Jia-Jun Liu, Feng Chen, Dong Jun Lin, Xiang Yuan Wu, Qu Lin, Xianglin Pan, Jun Peng, Ming Hou, and Ren Wei Huang
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Cancer Research ,Lung Neoplasms ,Survivin ,Blotting, Western ,Caspase 3 ,Apoptosis ,Mitochondrion ,In Vitro Techniques ,Caspase 8 ,Inhibitor of Apoptosis Proteins ,Membrane Potentials ,Downregulation and upregulation ,Cell Line, Tumor ,Animals ,Humans ,RNA, Messenger ,Enzyme Inhibitors ,Caspase-9 ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,Cytochrome c ,General Medicine ,Flow Cytometry ,Caspase 9 ,Mitochondria ,Neoplasm Proteins ,Enzyme Activation ,Oncology ,Biochemistry ,Proto-Oncogene Proteins c-bcl-2 ,Caspases ,Isodon ,biology.protein ,Cancer research ,Diterpenes ,Microtubule-Associated Proteins ,Drugs, Chinese Herbal ,Phytotherapy - Abstract
Ponicidin, an ent-kaurane diterpenoid derived from a constituent of the herbal supplement PC-SPES, Rabdosia rubescens, is recently reported to have anti-tumor effects on a large variety of cancers. In this study, we demonstrate that ponicidin exhibits cytotoxicity, induces apoptosis, disrupts the mitochondrial membrane potential, and triggers the activation of caspase-3, -8 and -9 in lung cancer A549 and GLC-82 cells. Ponicidin treatment of lung cancer cells caused downregulation of anti-apoptotic protein Bcl-2 and survivin as well as upregulaton of pro-apoptotic protein Bax in a time dependent manner when apoptosis ocurred. Ponicidin induced activation of caspase-3 can be blocked by a caspase-3-specific inhibitor z-DEVD-FMK Furthermore, the caspase-8-specific inhibitor z-IETD-FMK could block the ponicidin-induced activation of caspase-3, PARP cleavage, and prevented the release of cytochrome c from mitochondria into the cytoplasm. This indicate that activated caspase-8 initiates the release of cytochrome c during ponicidin-induced apoptosis. We therefore conclude that ponicidin has significant apoptosis-inducing effects by activation of caspase-3 -8, and -9 as well as downregulation of anti-apoptotic protein Bcl-2, survivin and upregulation of pro-apoptotic protein Bax, with caspase-8 acting as an upstream activator. The data offer a potential mechanism for ponicidin-induced apoptosis in lung cancer cells, suggesting that ponicidin may severve as an effective reagent for the treatment of lung cancer, and that in vivo anti-cancer effects as well as its potential clinical effectiveness need further investigation.
- Published
- 2006
21. Antiproliferation effects of oridonin on HPB-ALL cells and its mechanisms of action
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Xiangyuan Wu, Feng Chen, Qu Lin, Dong-Jun Lin, Jia-Jun Liu, Jun Peng, Ren-Wei Huang, Maohong Zhang, Ming Hou, and Xianglin Pan
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Programmed cell death ,Cell Survival ,Antineoplastic Agents ,Apoptosis ,Biology ,Membrane Potentials ,Cell Line, Tumor ,Humans ,MTT assay ,Viability assay ,Cell Proliferation ,Cell growth ,Caspase 3 ,Hematology ,Molecular biology ,Biochemistry ,Gene Expression Regulation ,Cell culture ,Caspases ,Cancer cell ,Mitochondrial Membranes ,DNA fragmentation ,Diterpenes ,Drug Screening Assays, Antitumor ,Apoptosis Regulatory Proteins ,Diterpenes, Kaurane - Abstract
Oridonin, an ent-kaurane diterpenoid derived from the herbal Rabdosia rubescens, has been recently reported to have antitumor effects on a large variety of cancer cells. The present study was undertaken to investigate the in vitro antiproliferation and apoptosis inducing effects of oridonin on HPB-ALL cell lines and its mechanisms of action. HPB-ALL cells in culture medium in vitro were treated with different concentrations of oridonin (16-56 micromol/L). MTT assay was used to detect the cell growth inhibitory rate, and the cell viability was assessed by the trypan blue dye-exclusion method. Cell apoptosis and the mitochondrial membrane potential (delta psi m) were investigated by flow cytometry (FCM), Hoechst 33258 staining, and DNA fragmentation analysis. The expression of caspase-3 and different apoptosis modulators, including Fas and Bcl-2 family members, was analyzed by Western blotting. The results revealed that oridonin could significantly inhibit the growth of HPB-ALL cells and cause apoptosis, and the suppression was both time- and dose-dependent. After treatment with oridonin for 48 hr, the percentage of disruption of delta psi m gradually increased in a dose-dependent manner along with marked changes of cell apoptosis, and necrotic cells increased remarkably after the cells were treated with oridonin for 72 hr; Western blotting showed cleavage of the caspase-3 zymogen protein (32 kDa) with the appearance of its 20-kDa subunit when apoptosis occurred; expression of Bcl-2 and Bcl-XL was downregulated remarkably while expression of Bax and Bid was upregulated concurrently after the cells were treated with oridonin for 24 hr. Of note, the expressions of Fas and other Bcl-2 family members including Bak and Bad remained constant before and after apoptosis occurred. We therefore conclude that oridonin has significant antiproliferation effects on HPB-ALL cells by induction of apoptosis as well as directly causing cell necrosis and that oridonin-induced apoptosis on HPB-ALL cells is mainly related to the disruption of delta psi m and activation of caspase-3 as well as downregulation of anti-apoptotic protein Bcl-2, Bcl-XL, and upregulation of pro-apoptotic proteins Bax and Bid. The results indicate that oridonin may serve as a potential antileukemia reagent.
- Published
- 2006
22. Apoptotic effect of oridonin on NB4 cells and its mechanism
- Author
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Ming Hou, Xiang-Yuan Wu, Feng Chen, Qu Lin, Xianglin Pan, Jun Peng, Maohong Zhang, Jia-Jun Liu, Dong-Jun Lin, and Ren-Wei Huang
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Cancer Research ,Blotting, Western ,Antineoplastic Agents ,Apoptosis ,Biology ,Flow cytometry ,chemistry.chemical_compound ,Leukemia, Promyelocytic, Acute ,Cell Line, Tumor ,medicine ,Humans ,MTT assay ,Cell Proliferation ,medicine.diagnostic_test ,Dose-Response Relationship, Drug ,Molecular Structure ,Cell growth ,Caspase 3 ,Hematology ,Molecular biology ,Blot ,Oncology ,chemistry ,Proto-Oncogene Proteins c-bcl-2 ,Cell culture ,Caspases ,DNA fragmentation ,Trypan blue ,Diterpenes ,Drug Screening Assays, Antitumor ,Diterpenes, Kaurane - Abstract
The anti-proliferation effects of oridonin on acute promyelocytic leukemia (APL) cells and its mechanisms were studied in vitro. NB4 cells as well as fresh leukemia cells obtained from APL patients in culture medium were treated with different concentrations of oridonin. Cell growth inhibition, apoptosis and related pathways were assessed by MTT assay as well as flow cytometry (FCM) and western blot analysis. The data revealed that oridonin (over 16 micromol/L) could inhibit the growth of NB4 cells by induction of apoptosis. Marked changes of cell apoptosis were observed very clearly by using electron microscopy and DNA fragmentation analysis after the cells exposed to oridonin for 48 h; Western blotting showed cleavage of the caspase-3 zymogen protein (32-kDa) with the appearance of its 20-kDa subunit as well as a cleaved 89-kDa fragment of 116-kDa PARP when apoptosis occurred. The expression of Bcl-2 was down-regulated remarkably accompanied by the disruption of the mitochondrial membrane potential (delta(psi)m). The anti-proliferative and apoptosis-inducing effects by oridonin in fresh APL cells were also found remarkably using Trypan Blue dye exclusion method and Wright's staining. We concluded that oridoning has significant anti-proliferative and apoptosis-inducing effects on NB4 cells by activation of caspase-3 and cleavage of PARP as well as by down regulation of Bcl-2 and disruption of the delta(psi)m. Furthermore, oridonin demonstrated apparent cell growth inhibition effects on fresh APL cells in vitro. The results indicated that oridonin may serve as a potential anti-leukemia reagent.
- Published
- 2005
23. Antiproliferation effects of ponicidin on human myeloid leukemia cells in vitro
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Ren-Wei Huang, Xianglin Pan, Feng Chen, Dong-Jun Lin, Maohong Zhang, Qu Lin, Yu-Qin Song, Jun Peng, Xiang-Yuan Wu, Jia-Jun Liu, and Ming Hou
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Cancer Research ,Time Factors ,Cell Survival ,Blotting, Western ,Down-Regulation ,Antineoplastic Agents ,Apoptosis ,HL-60 Cells ,DNA Fragmentation ,Biology ,In Vitro Techniques ,Humans ,MTT assay ,Viability assay ,Cell Proliferation ,bcl-2-Associated X Protein ,Dose-Response Relationship, Drug ,Caspase 3 ,Plant Extracts ,Myeloid leukemia ,General Medicine ,Cell cycle ,Flow Cytometry ,Up-Regulation ,Blot ,Oncology ,Models, Chemical ,Proto-Oncogene Proteins c-bcl-2 ,Leukemia, Myeloid ,Caspases ,Cancer research ,Bisbenzimidazole ,DNA fragmentation ,Diterpenes ,Poly(ADP-ribose) Polymerases ,K562 Cells ,K562 cells ,Drugs, Chinese Herbal - Abstract
Ponicidin, an extract from the Chinese herb Rabdosia rubescens, is currently one of the most important traditional Chinese herbal medicines. Ponicidin has been reported to have anti-tumor effects on a large variety of malignant diseases. In this study, we investigated the anti-proliferation effects of ponicidin on human myeloid K562 and HL-60 cells. Cell viability was measured by MTT assay; cell apoptosis was assessed by flow cytometry, DNA fragmentation analysis and Hoechst 33258 staining. Caspase-3 and poly(ADP-ribose) polymerase (PARP) activation and Bax and Bcl-2 expression were detected by Western blot analysis. The results revealed that ponicidin could significantly inhibit the growth of K562 and HL-60 cells by induction of apoptosis. The suppression was both time- and dose-dependent. Cell apoptosis was observed clearly after the cells were treated with ponicidin for 48-72 h. Western blotting showed cleavage of the caspase-3 zymogen protein (32 kDa) with the appearance of its 17 kDa subunit, together with a cleaved 89-kDa fragment of 116 kDa PARP when apoptosis occurred. Bcl-2 expression was down-regulated while Bax expression up-regulated concurrently when the cells were treated with ponicidin for 24-48 h. Therefore, we conclude that ponicidin has significant anti-proliferation effects by induction of apoptosis on myeloid leukemia cells in vitro, down-regulation of Bcl-2, and up-regulation of Bax, and that activation of caspase-3 and PARP may be an important apoptosis-inducing mechanism. The results suggest that ponicidin may serve as a potential therapeutic agent for leukemia.
- Published
- 2005
24. Peroxisome proliferator activated receptor-gamma ligands induced cell growth inhibition and its influence on matrix metalloproteinase activity in human myeloid leukemia cells
- Author
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Ren-Wei Huang, Huiling Lu, Yuqin Song, Jun Peng, Dong-Jun Lin, Jing Zheng, Maohong Zhang, Ming Hou, X. Wu, Xiangyuan Wu, Feng Chen, Qu Lin, Jia-Jun Liu, and Xianglin Pan
- Subjects
Cancer Research ,Antineoplastic Agents ,Biology ,Toxicology ,Extracellular matrix ,chemistry.chemical_compound ,Troglitazone ,medicine ,Tumor Cells, Cultured ,Humans ,Pharmacology (medical) ,Chromans ,Cell adhesion ,Pharmacology ,Matrigel ,Cell growth ,Prostaglandin D2 ,Myeloid leukemia ,medicine.disease ,Extracellular Matrix ,PPAR gamma ,Leukemia ,Oncology ,chemistry ,Leukemia, Myeloid ,Cancer research ,Metalloproteases ,Thiazolidinediones ,Growth inhibition ,K562 cells - Abstract
Peroxisome proliferator-activated receptor-gamma (PPAR-gamma) is one of the best characterized nuclear hormone receptors (NHRs) in the superfamily of ligand-activated transcriptional factors. PPAR-gamma ligands have recently been demonstrated to affect proliferation, differentiation and apoptosis of different cell types. The present study was undertaken to investigate PPAR-gamma ligands induced cell growth inhibition and its influence on matrix metalloproteinase MMP-9 and MMP-2 activities on leukemia K562 and HL-60 cells in vitro. The results revealed that PPAR-gamma expression was detectable in the two kinds of leukemia cells; Both 15-deoxy-delta(12,14)-prostaglandin J2(15d-PGJ2) and troglitazone (TGZ) have significant growth inhibition effects on these two kinds of leukemia cells. These two PPAR-gamma ligands could inhibit the leukemic cell adhesion to the extracellular matrix (ECM) proteins and the invasion through matrigel matrix. The expressions of MMP-9 and MMP-2 as well as their gelatinolytic activities in both HL-60 and K562 cells were inhibited by 15d-PGJ2 and TGZ significantly. We therefore conclude that PPAR-gamma ligands 15d-PGJ2 and TGZ have significant growth inhibition effects on myeloid leukemia cells in vitro, and that PPAR-gamma ligands can inhibit K562 and HL-60 cell adhesion to and invasion through ECM as well as downregulate MMP-9 and MMP-2 expressions. The data suggest that PPAR-gamma ligands may serve as potential anti-leukemia reagents.
- Published
- 2004
25. [Effects of Notch and its ligands on the differentiation of 32D cell]
- Author
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Chunyan, Ji, Daoxin, Ma, Jianqiang, Zhao, and Maohong, Zhang
- Subjects
Receptors, Notch ,Calcium-Binding Proteins ,Membrane Proteins ,Cell Differentiation ,CHO Cells ,Transfection ,Cricetulus ,Cricetinae ,Animals ,Intercellular Signaling Peptides and Proteins ,Serrate-Jagged Proteins ,Receptor, Notch2 ,Receptor, Notch1 ,Signal Transduction - Abstract
To explore the mechanism of Notch signaling transduction system and its effects on hematopoietic system.Notch ligands transfected CHO cells were added into Notch1 and Notch2 transfected CHO cells, which were transiently transfected with reporter gene TP1. PGL-100 was used as substrate to test the interaction between Notch and Notch ligands. CHO, Jagged2-CHO and Delta 4-CHO cells were seeded in the petri dish containing G-CSF, and then Notch 1-32D cells were added in it to observe the differentiation of Notch1-32D cell after incubation and staining.All of the five Notch ligands binding to Notch1 could induce TP1 activity, it increased significantly the Jagged2-CHO, Delta 4-CHO1-4 and Delta 4-CHO1-5 cells. For Notch2, the TP1 activity induced by the five ligands in these cells was much higher than that of CHO. At the presence of G-CSF, Notch1-32D could differentiate to mature granulocyte. Jagged2 could inhibit G-CSF induced Notch1-32D cell differentiation, but Delta 4 could not.Jagged2 and Delta 4 are the ligands of Notch1. Jagged2 can inhibit G-CSF induced Notch1-32D cell differentiation, but Delta 4 can not.
- Published
- 2003
26. [Participation of bonth splenic CD(5)(+) and CD(5)(-) B lymphocytes in production of platelet glycoprotein-specific autoantibodies in chronic ITP]
- Author
-
Baojun, Lu, Ming, Hou, Lu, Lu, Yan, Shi, Qingsi, He, Daoxin, Ma, and Maohong, Zhang
- Subjects
Adult ,Male ,B-Lymphocytes ,Purpura, Thrombocytopenic, Idiopathic ,Adolescent ,Platelet Glycoprotein GPIIb-IIIa Complex ,Middle Aged ,CD5 Antigens ,Platelet Glycoprotein GPIb-IX Complex ,Chronic Disease ,Humans ,Female ,Spleen ,Autoantibodies - Abstract
To investigate the percentage of splenic CD(5)(+) B lymphocytes in chronic idiopathic thrombocytopenic purpura (IT) and the impact of splenic CD(5)(+) and CD(5)(-) B lymphocytes on the production of platelet glycoprotein (GP)-specific autoantibodies.Splenic CD(5)(+) B lymphocytes were identified by two-color flow cytometric analysis in eight patients. Four of the eight patients displayed plasma autoantibodies against both GPIIb/IIIa and GPIb/IX, and their splenic B lymphocytes were separated by Ficoll-Hypaque density gradient and sheep erythrocyte, and further purified by magnetic activate cell separation (MACS). Purified CD(5)(+) and CD(5)(-) B lymphocytes were cultured separately with or without staphylococcus aureus cowan I (SAC). GP specific autoantibodies in culture supernatants were measured by modified monoclonal antibody immobilization of platelet antigen assay (MAIPA).The percentage of splenic CD(5)(+) B lymphocytes in ITP patients was slightly higher than that in control with no statistical significance. MACS purified splenic CD(5)(+) and CD(5)(-) B lymphocytes from three out of four ITP patients produced high levels of anti-GPIIb/IIIa and anti-GPIb/IX antibodies. Culture supernatants of CD(5)(+) B lymphocytes from the other patient showed positive reaction only in GPIb/IX MAIPA. Culture supernatant of CD(5)(-)B lymphocytes from the same patient were double positive in both GPIIb/IIIa and GPIb/IX MAIPA.Both splenic CD(5)(+) and CD(5)(-) B lymphocytes produce platelet GP-specific autoantibodies in chronic ITP with similar antibody spectrum and titer, and may all play a role in the autoimmune pathogenesis of ITP.
- Published
- 2002
27. Non-leukemic myeloid sarcoma involving the vulva, vagina, and cervix: a case report and literature review.
- Author
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Yuan Yu, Xuemei Qin, Shuxin Yan, Wenxia Wang, Yanlin Sun, and Maohong Zhang
- Subjects
MYELOID leukemia ,CANCER chemotherapy ,GENITALIA ,BONE marrow ,ANTIGENS ,CANCER patients - Abstract
Abstract: Myeloid sarcoma (MS) is defined as a tumor mass consisting of myeloid blast with or without maturation occurring at an anatomical site other than bone marrow with normal architectural effacement. It can also precede the onset of leukemia which is called non-leukemic MS. Non-leukemic MS is a kind of rare disease and easy to be misdiagnosed as other common malignancies due to the rarity and nonspecific manifestation. We herein report an unusual case of non-leukemic MS involving the vulva, vagina, and cervix in a female patient. The bone marrow aspiration and biopsy of the patient revealed no hematological abnormality. Immunohistochemical staining of the biopsies was strongly positive for myeloperoxidase, CD68, leukocyte common antigen (LCA), CD117, CD34, CD38, CD79a, and negative for cytokeratin (CK), epithelial memberane antigen (EMA), CD2, CD3, CD20, CD5, CD138. Then a diagnosis of non-leukemic MS was made. Unfortunately, our patient received only one cycle of chemotherapy consisting of cytosine arabinoside and daunorubicin, then refused any further treatment and died 4 months after diagnosis. Although systemic chemotherapy is widely accepted to be a promising strategy, its benefit still needs to be further assessed. Certain questions still need to be answered for this disease: 1) Why can approximately 20% of the patients with non-leukemic MS remain disease-free after local therapy alone? 2) How many cycles of chemotherapy are needed for these patients after achievement of complete remission? 3) What are the prognostic or risk factors in these patients who have no abnormality of karyotype, fusion genes, or gene mutation to predict responsiveness to chemotherapy and outcome? 4) What is the risk factor for relapse? The rarity of non-leukemic MS makes it almost impossible to conduct large-scale randomized trials, but judicious study for each patient with MS is helpful for a further understanding of the nature of the disease. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
28. Antithrombin III activity in Chinese women with preeclampsia
- Author
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Maohong Zhang, Shukun Wang, Conggao Xu, Lin Zhou, Shuqin Shong, and Carl P. Weiner
- Subjects
Antithrombin III Activity ,medicine.medical_specialty ,Pregnancy ,China ,biology ,business.industry ,Antithrombin III ,Hematology ,medicine.disease ,Preeclampsia ,Text mining ,Endocrinology ,Pre-Eclampsia ,Enzyme inhibitor ,Internal medicine ,medicine ,biology.protein ,Gestation ,Humans ,Female ,Prospective Studies ,business - Published
- 1990
29. The research of city community safety management system.
- Author
-
Lizhen Liu, Maohong Zhang, Lei Chen, and Chao Du
- Published
- 2010
- Full Text
- View/download PDF
30. Peroxisome proliferator activated receptor-γ ligands induced cell growth inhibition and its influence on matrix metalloproteinase activity in human myeloid leukemia cells.
- Author
-
Jiajun Liu, Huiling Lu, Renwei Huang, Dongjun Lin, Xiangyuan Wu, Qu Lin, Xinyao Wu, Jing Zheng, Xianglin Pan, Jun Peng, Yuqin Song, Maohong Zhang, Ming Hou, and Feng Chen
- Subjects
ORGANELLE formation ,PEROXISOMES ,LIGANDS (Biochemistry) ,CELL growth ,METALLOPROTEINASES ,MYELOID leukemia ,CANCER cells ,APOPTOSIS - Abstract
Peroxisome proliferator-activated receptor-gamma (PPAR-γ) is one of the best characterized nuclear hormone receptors (NHRs) in the superfamily of ligand-activated transcriptional factors. PPAR-γ ligands have recently been demonstrated to affect proliferation, differentiation and apoptosis of different cell types. The present study was undertaken to investigate PPAR-γ ligands induced cell growth inhibition and its influence on matrix metalloproteinase MMP-9 and MMP-2 activities on leukemia K562 and HL-60 cells in vitro. The results revealed that PPAR-γ expression was detectable in the two kinds of leukemia cells; Both 15-deoxy-delta(12,14)-prostaglandin J2(15d-PGJ2) and troglitazone (TGZ) have significant growth inhibition effects on these two kinds of leukemia cells. These two PPAR-γ ligands could inhibit the leukemic cell adhesion to the extracellular matrix (ECM) proteins and the invasion through matrigel matrix. The expressions of MMP-9 and MMP-2 as well as their gelatinolytic activities in both HL-60 and K562 cells were inhibited by 15d-PGJ2 and TGZ significantly. We therefore conclude that PPAR-γ ligands 15d-PGJ2 and TGZ have significant growth inhibition effects on myeloid leukemia cells in vitro, and that PPAR-γ ligands can inhibit K562 and HL-60 cell adhesion to and invasion through ECM as well as downregulate MMP-9 and MMP-2 expressions. The data suggest that PPAR-γ ligands may serve as potential anti-leukemia reagents. [ABSTRACT FROM AUTHOR]
- Published
- 2005
- Full Text
- View/download PDF
31. Apoptotic effect of oridonin on NB4 cells and its mechanism.
- Author
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Jiajun Liu, Renwei Huang, Dongjun Lin, Xiangyuan Wu, Jun Peng, Qu Lin, Xianglin Pan, Maohong Zhang, Ming Hou, and Feng Chen
- Subjects
CELLULAR mechanics ,APOPTOSIS ,LEUKEMIA ,CANCER ,ELECTRON microscopy ,CELL membranes - Abstract
The anti-proliferation effects of oridonin on acute promyelocytic leukemia (APL) cells and its mechanisms were studied in vitro. NB4 cells as well as fresh leukemia cells obtained from APL patients in culture medium were treated with different concentrations of oridonin. Cell growth inhibition, apoptosis and related pathways were assessed by MTT assay as well as flow cytometry (FCM) and western blot analysis. The data revealed that oridonin (over 16?µmol/L) could inhibit the growth of NB4 cells by induction of apoptosis. Marked changes of cell apoptosis were observed very clearly by using electron microscopy and DNA fragmentation analysis after the cells exposed to oridonin for 48?h; Western blotting showed cleavage of the caspase-3 zymogen protein (32-kDa) with the appearance of its 20-kDa subunit as well as a cleaved 89-kDa fragment of 116-kDa PARP when apoptosis occurred. The expression of Bcl-2 was down-regulated remarkably accompanied by the disruption of the mitochondrial membrane potential (??m). The anti-proliferative and apoptosis-inducing effects by oridonin in fresh APL cells were also found remarkably using Trypan Blue dye exclusion method and Wright's staining. We concluded that oridoning has significant anti-proliferative and apoptosis-inducing effects on NB4 cells by activation of caspase-3 and cleavage of PARP as well as by down regulation of Bcl-2 and disruption of the ??m. Furthermore, oridonin demonstrated apparent cell growth inhibition effects on fresh APL cells in vitro. The results indicated that oridonin may serve as a potential anti-leukemia reagent. [ABSTRACT FROM AUTHOR]
- Published
- 2005
- Full Text
- View/download PDF
32. The Construction and Application of Hybrid Teaching Model of Flipping Classroom and Divided Classroom Base on Project-driven in the Teaching of Java programming.
- Author
-
Maohong Zhang, Zhenmei Yu, Yuhong Sun, Xueyan Gong, and Yuan Liu
- Published
- 2020
- Full Text
- View/download PDF
33. Research on Topic Mining Algorithm Based on Deep Learning Extension.
- Author
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Fuqiang Yang, Xuechen Zhao, and Maohong Zhang
- Published
- 2019
- Full Text
- View/download PDF
34. Research on the Theory of Multiple Intelligences in Training Mode of College Students’ Innovative Ability.
- Author
-
Maohong Zhang, Hui Liu, and Lihua Sun
- Published
- 2019
- Full Text
- View/download PDF
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