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13 results on '"Mao, Lanfang"'

5. Pingwei capsules improve gastrointestinal motility in rats with functional dyspepsia

Author

Liu Junhong, Li Hongfang, Du Xiaojuan, Liang Jingjing, Li Yunxia, Wang Long-de, Liang Qiankun, Yan Yuan, and Mao Lanfang

Subjects
0301 basic medicine, medicine.medical_specialty, medicine.medical_treatment, Motility, digestive system, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, medicine, Saline, Antrum, Cholecystokinin, business.industry, Leptin, digestive, oral, and skin physiology, General Medicine, Domperidone, 030104 developmental biology, medicine.anatomical_structure, Endocrinology, Hypothalamus, 030220 oncology & carcinogenesis, Duodenum, business, hormones, hormone substitutes, and hormone antagonists, medicine.drug
Abstract

OBJECTIVE To investigate the mechanism of Pingwei capsules (PWC) in improving gastrointestinal motility in rats with functional dyspepsia (FD). METHODS We established an FD model by stimulating semi-starvation rats via tail damping, provocation, and forced exercise fatigue. The FD model group was further divided into five groups according to the treatment received: normal saline, domperidone, low-dose PWC, mid-dose PWC, or high-dose PWC. The effect of PWC on FD rat was evaluated by measuring gastrointestinal motility. Changes of leptin and cholecystokinin (CCK) were detected through enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, and immunohistochemistry. RESULTS PWC significantly increased gastrointestinal motility in FD rats. Furthermore, PWC significantly increased CCK mRNA and protein concentrations in the duodenum and antrum, decreased leptin protein concentrations in the duodenum, antrum, and hypothalamus, and decreased CCK protein concentration in the hypothalamus. CONCLUSION PWC improve gastrointestinal motor function in FD rats by decreasing the leptin concentration in serum and the brain-gut axis, and by increasing the CCK concentration in gastrointestinal tissue. Our findings help to elucidate the mechanism of FD and provide further insight into the pharmacokinetics of PWC.

Published
2020

13. Evaluation of a modified rat model for functional dyspepsia.

Author

Liang Q, Yan Y, Mao L, Du X, Liang J, Liu J, Wang L, and Li H

Subjects
Animals, Cholecystokinin blood, Disease Models, Animal, Gastric Emptying physiology, Hypothalamus metabolism, Leptin blood, Male, Motilin blood, Rats, Rats, Wistar, Vasoactive Intestinal Peptide blood, Dyspepsia blood, Dyspepsia physiopathology, Gastric Mucosa metabolism, Gastrointestinal Hormones blood, Gastrointestinal Motility physiology, Stomach physiopathology
Abstract

Background/aim: The purpose of this study was to establish a modified rat model with functional dyspepsia (FD) and analyze the changes in gastrointestinal motility and brain-gut peptide levels in serum and brain-gut axis., Materials and Methods: Male Wistar rats were divided into control group (Con) and FD model group. FD model was established by stimulating semi-starvation rats via tail damping, provocation, and forced exercise fatigue until gastrointestinal motility disorder appeared, and then levels of motilin, leptin, cholecystokinin (CCK), and vasoactive intestinal peptide (VIP) were detected in serum by enzyme linked immunosorbent assay and in duodenum, antrum, and hypothalamus by immunohistochemistry, reverse transcriptase-polymerase chain reaction, and Western blot., Results: The results showed rates of intestinal propulsion and gastric emptying slowed down markedly compared to Con (P < 0.05), the gastrointestinal electric activity attenuated, and migrating motor complex (MMC) interrupted in the model group. The levels of leptin and VIP markedly increased, but motilin decreased as compared to the Con (P < 0.05) in serum and in the above tissues. It is interesting that the level of CCK decreased in the antrum and duodenum but increased in the hypothalamus as compared to Con (P < 0.05)., Conclusions: The modified rat model meets the diagnostic criteria of FD and can be used as a method for studying FD in animals., Competing Interests: There are no conflicts of interest

Published
2018
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