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Abstract [Objective] This study aims to identify and analyze the members of the tonoplast intrinsic protein (TIP) subfamily in apple, and to investigate the expression patterns under drought stress. It provides information for further research on utilization of drought resistance gene resources in apple. [Methods] The MdTIPs in apple genome was identified by bioinformatics methods. The physicochemical properties, gene structures, conserved motifs, cis-regulatory elements, and phylogenetic trees, etc. of the subfamily members were analyzed. The expression patterns of MdTIPs in different organs under drought stress were analyzed by qRT-PCR. [Results] A total of 13 MdTIP genes were identified in the apple genome, and most members were localized at the plasma membrane. Chromosome localization analysis suggested that all members were distributed on 10 chromosomes, with 1-3 members on each chromosome. Besides, the promoter regions of the genes contained response elements for hormonal and adversity stresses. qRTPCR showed that MdTIP members were up-regulated in roots except MdTIP1;1, of which MdTIP1;3 and MdTIP1;4 were up-regulated 5.27 times and 5.69 times, respectively, compared with the control, suggesting that these two genes might be critical in response to drought stress. [Conclusion] Identification of MdTIP subfamily members is provided in this study. 10 MdTIP members are differentially expressed in roots, stems, and leaves, and 12 members are highly expressed in roots.

The water extract contents, polyphenol compositions, total free amino acid contents, caffeine (CAF) contents, amino acid compositions and catechin compositions of spring and summer tea made from six varieties planted in Motuo, namely Tezao 213, Zhuyeqi, Zhongcha 302, Tieguanyin, Yinghong 9 and Fenghuang Dancong were determined and compared. The results indicated significant differences between spring and summer tea in all quality components except tea polyphenols. The average phenol/ammonia ratio of spring and summer tea was 4.60 and 7.99, respectively, indicating summer tea to be suitable for processing into green and black tea. Altogether 18 amino acids were detected in the six cultivars, and the contents of different groups of amino acid were in the decreasing order of umami amino acids > sweet amino acids > bitter amino acids > aromatic amino acids. The contents of umami and sweet amino acids in spring tea were significantly higher than those in summer tea. Correlation analysis revealed that the synthesis of various metabolites were highly correlated. Principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) showed that season had a more significant effect on tea metabolites than variety. A total of 16 compounds were identified as differential metabolites between spring and summer tea, including 7 amino acids, 8 catechins and CAF, which could be used as potential markers to distinguish spring and summer tea. This study provides a valuable reference for tea processing.

[Objective] The study aims to explore the effects of different foliar fertilizers on the photosyn thetic characteristics, fruit quality, and yield of greenhouse strawberries, and to screen suitable foliar fer tilizers. [Methods] The strawberry variety “Monterey” planted in the greenhouse of Moujiatai Strawberry in Xigu District of Lanzhou City, Gansu Province was used as test materials. Under sufficient fertilization, five types of foliar fertilizers, including classic 750×liquid (T1), Huapu 1 500×liquid (T2), and Runliang 750×liquid (T3), 500×Adela fruit (T4), and 1500×potassium dihydrogen phosphate (T5), were ap plied topdressing at four growth stages, with water as control. The photosynthetic parameters, fruit qual ity, biomass, and yield were measured. [Results] (1) After spraying different foliar fertilizers, the bio mass and chlorophyll content of strawberry plants were significantly increased, and the net photosynthetic rate and transpiration rate of leaves were significantly increased, while the intercellular carbon dioxide con centration was higher than that of CK. (2) For fruit quality, T2 and T3 treatments significantly improved the strawberry fruit shape index, while T1 and T5 treatments significantly increased fruit volume and weight. Among them, the average single fruit mass of T1 was increased by 35.04% compared with CK, while the transverse and longitudinal diameters of T5 fruits were increased by 49.39% and 40.64%, re spectively. (3) The quality of strawberry fruits was effectively improved by various foliar fertilizer treat ments, and T1 treatment was the best. The content of vitamin C, soluble solids, soluble proteins, reduc ing sugars, and soluble sugars were increased by 30.84%, 33.5%, 38.5%, 27.9%, and 36.2%, respec tively, compared with CK. The content of titratable acid was the lowest, and the sugar acid ratio was the highest, with significant difference compared with CK. (4) Spraying appropriate fertilizer significantly in creased plant biomass, max single fruit weight, and fruit yield; treatment T1 had the highest yield, an in crease of 13.65% compared with CK. [Conclusion] The application of appropriate foliar fertilizer effective ly improves the nutritional growth status of strawberries, enhances the quality of strawberry fruits, and significantly increases yield. The comprehensive effect is best when applying classic 750×liquid.

Objective To investigate the rehabilitation effects of vestibular rehabilitation in acute brainstem infarction patients with subjective visual vertical (SVV) tilting, and the correlation between the SVV, balance confidence, dizziness/vertigo and walking function. Methods All 45 acute brainstem infarction patients with SVV tilting who were hospitalized in Tianjin Huanhu Hospital from July 2022 to July 2023 were collected. Patients were divided into vestibular rehabilitation group (n = 25) and general rehabilitation group (n = 20), Bucket Test was used to test SVV tilting angle, Activities-Specific Balance Confidence Scale (ABC) was used to evaluate balance confidence, Visual Analog Scales (VAS) was used to evaluate dizziness/vertigo and Functional Ambulation Category Scale (FAC) was used to evaluate walking function before and after 2 weeks of treatment. Results The SVV tilting angle (F = 4.356, P = 0.043), ABC score (F = 4.389, P = 0.042), dizziness/vertigo VAS score (F = 4.138, P = 0.048) were significantly different between vestibular rehabilitation group and general rehabilitation group. After treatment, the SVV tilting angle (t = - 2.139, P = 0.038) and the dizziness/vertigo VAS score (t = - 2.952, P = 0.005) in vestibular rehabilitation group were lower than those in general rehabilitation group, and the ABC score was higher than that in general rehabilitation group (t = 2.920, P = 0.006). SVV tilting angle (F = 196.923, P = 0.000), ABC score (F = 89.050, P = 0.000), dizziness/vertigo VAS score (F = 81.803, P = 0.000), FAC grade (F = 72.866, P = 0.000) were statistically significant. The SVV tilting angle (t = 0.763, P = 0.000; t = 0.972, P = 0.000) and dizziness/vertigo VAS score (t = 8.815, P = 0.000; t = 5.107, P = 0.000) after treatment were lower than those before treatment of 2 groups, and the ABC score (t = 0.689, P = 0.001; t = 0.703, P = 0.001) and FAC grade (t = - 6.721, P = 0.000; t = - 5.772, P = 0.000) were higher than those before treatment of 2 groups. Correlation analysis showed a positive correlation between SVV tilting angle and dizziness/vertigo VAS score in acute brainstem infarction patients with SVV tilting (r = 0.627, P = 0.000). Conclusions Vestibular rehabilitation can effectively improve the SVV tilting, balance confidence and dizziness/vertigo symptoms of acute brainstem infarction patients with SVV tilting, and the SVV tilting angle is closely related to the degree of dizziness/vertigo.

The PIN‐FORMED (PIN) proteins mediate the auxin flow throughout the plant and have been identified in many species. However, evolution differences in the PIN gene families have not been systematically analyzed, and their functions under abiotic stresses in grape are largely unexplored. In this study, 373 PIN genes were identified from 25 species and divided into 3 subgroups. Physicochemical properties analysis indicated that most of the PIN proteins were unstable alkaline hydrophobic proteins in nature. The synteny analysis showed that the PINs contained strong gene duplication. Motif composition revealed that PIN gene sequence differences between monocotyledons and dicotyledons were due to evolutionary‐induced base loss, and the loss was more common in dicotyledonous. Meanwhile, the codon usage bias showed that the PINs showed stronger codon preference in monocotyledons, monocotyledons biased towards C3s and G3s, and dicotyledons biased towards A3s and T3s. In addition, the VvPIN1 can interact with VvCSN5. Significantly, under freezing treatment, the ion leakage, O2·−$$ \left({O}_2^{\cdotp -}\right) $$, H2O2, and malondialdehyde (MDA) were obviously increased, while the proline (Pro) content, peroxidase (POD) activity, and glutathione (GSH) content were decreased in VvPIN1‐overexpressing Arabidopsis compared to the wild type (WT). And quantitative real‐time PCR (qRT‐PCR) showed that AtICE1, AtICE2, AtCBF1, AtCBF2, and AtCBF3 were down‐regulated in overexpression lines. These results demonstrated that VvPIN1 negatively regulated the freezing tolerance in transgenic Arabidopsis. Collectively, this study provides a novel insight into the evolution and a basis for further studies on the biological functions of PIN genes in monocotyledons and dicotyledons. [ABSTRACT FROM AUTHOR]